Mass spectrometry (MS) as an analytical technique enables the identification and quantitative determination of proteins, metabolites, or lipids in a studied sample. However, this method has limitations regarding the number of molecules that can be identified at a given time. To increase the number of identifications, the application of ion mobility spectrometry (IMS) can be employed. This technique allows the separation of ions based on their mobility while traversing the analyser in a gradient of an electromagnetic field and opposing gas pressure. The separation is performed in conjunction with MS analysis, adding another dimension to the analysis, resulting in a significant improvement in the number of identified compounds and a reduction in noise. Alternatively, while maintaining the same number of identifications, analysis can be performed in a shorter time period. It is crucial to pay special attention to the type of IMS analyser used, as its specific implementation dictates further stages of analysis and ion detection capabilities.