Quantitative Composition Of Microflora Research Articles

Functional activity of the oral endothelium in persons, with chronic periodontitis during treatment with plasmolifting

Chronic periodontitis as an osteoimmune disease of the oral cavity is accompanied by a change in the functional activity of endotheliocytes. Moreover, abnormal vascularization exacerbates periodontal inflammation, as it promotes the transmigration of a larger number of immunocompetent cells, the influx of inflammatory mediators and cytokines.The aim of our work was to study the functional activity of the endothelium of the vessels of the oral cavity in persons suffering from chronic periodontitis in the treatment of plasmolifting.Materials and methods. Under observation were 30 patients diagnosed with chronic generalized periodontitis of moderate severity at the age of 35 (32.50; 40.00) years, with no severe somatic pathology (main group). The comparison group included 20 people aged 38 (34.00; 45.00) years with no inflammatory diseases in the oral cavity. All patients underwent local anti-inflammatory therapy and sanitation of periodontal pockets, correction of occlusal contacts, curettage, plasma lifting. Oral fluid concentration of soluble adhesion molecules ICAM-1 and VCAM-1, endothelin-1, qualitative and quantitative composition of microflora were determined.Results. After the treatment with plasmolifting, a noticeable relief of the activity of the inflammatory process was observed. In patients with chronic periodontitis, Porphyromonas gingivalis was found in 100 % of cases in a titer of 5.73 (4.9; 6.7) lg (gEq/sample), in 62.5 % – Prevotella intermedia in a titer of 4.5 (3.0; 5.5) lg (gEq/sample). Against the background of therapy, decrease of the occurrence of the microorganism and of the number of microorganisms was observed. The concentration of the soluble form of VCAM-1 in the oral fluid of patients with chronic periodontitis exceeded the values of the control group by 38.3 times (p = 0.000001), and ICAM-1 – by 18.1 times (p = 0.00001). Against the background of plasmolifting therapy, the level of the studied substances decreased, but exceeded the control values by 25.2 and 6.4 times, respectively. The content of endothelin in the oral fluid in patients with periodontitis exceeded the values of healthy individuals by 40.7 % (p = 0.003), during therapy its values decreased, but did not reach the level of healthy volunteers (p = 0.04).

VARIETY OF DIAGNOSED MICROORGANISMS IN PATIENTS WITH ERYSIPELAS

Purpose. The purpose is to find out the frequency of surgical forms of erysipelas among the general population of the disease.
 Materials and methods. Traditionally, there is a widespread point of view about streptococcal etiology of erysipelas. However, recently, staphylococcus or microbial associations are increasingly sown from the focus of inflammation in patients.
 The relevance of studying the course of erysipelas is determined by the tendency to relapse, a significant increase in complications of the disease in recent years and the final phenomena.
 The existing clinical situation is largely explained by changes in the spectrum of pathogens of purulent surgical infection of soft tissues. Its leading pathogens are mainly staphylococci and streptococci, as well as gram - negative aerobic bacteria.
 The nature of the clinical course of the disease, including the nature of the local inflammatory focus, depends on the totality of the pathogenic properties of microbes that form the microbial landscape.
 It was found that most often the local focus was localized on the lower extremities.
 114 case histories of patients who were hospitalized in the center of purulent-septic surgery in the Non-profit municipal enterprise “City Hospital № 3” in Zaporizhzhia for the period 2019-2020 were analyzed retrospectively and prospectively. All patients were diagnosed with erysipelas on admission, according to the forms: erythematous - 24 (21.0%), bullous - 28 (24.6%), phlegmonous form - 48 (42.1%), necrotic - 14 (12.3%). %). Disease relapses were noted in 21 patients. In terms of gender: men - 47 (41.2%), women - 67 (58.8%). The patients' age was 62.1 ± 2.6 years. Automated devices "Vitek-2" or "BaCT ALERT" (France) were used to determine the sensitivity of microorganisms in a purulent focus to antibacterial drugs. Statistical analysis was performed using the “Statgraphics Plus for Windows 7.0” software package.
 Research results and their analysis. It was found that most often the local focus was localized on the lower extremities. A total of 26 types of bacteria were identified from the wound contents of patients with destructive forms of erysipelas. No bacterial growth was found in 3 (4.8%) patients, and mixed flora was isolated in 27 (45.8%) patients. Gram-positive flora accounted for 61.0%, gram-negative flora - 35.6%, anaerobes - were not found, fungi - 3.4%.
 Conclusions. The number of destructive forms of erysipelas among the general population of the disease is more than 50%. The study of purulent-necrotic foci in patients with destructive forms of erysipelas makes it possible to determine the qualitative and quantitative composition of microflora, sensitivity to antibiotics, and make adjustments to the conduction of antibiotic therapy.

Quantification of the health-improving action of phyto modules in the rooms of child care preschool facilities

The effect of three phyto modules – different combinations of indoor plants with the pronounced phytoncide activity – on the qualitative and quantitative composition of microflora in the air and on a decrease in the risk of acute respiratory diseases (ARD) in children was studied during autumn and winter in 2018-2020 in child care preschool institutions. Sanitary descriptive, epidemiological, sanitary bacteriological and statistical methods were involved. Observation revealed higher attendance rate in the observation group, in combination with lower recorded incidence of disease in comparison with the reference group. The fraction of facultative microflora (FM) with respect to the total microbial count (TMC) was about 30 % in the rooms equipped with phyto modules, while the ratio was 60% in the rooms without plants. The degree of antimicrobial activity depended on the assortment of plants and on the total leaf area per unit room volume. The phytoncide effect of the plants was traced to the most remote point of the room under investigation – 3 m. A favorable factor was an increase in the relative air humidity from 26 to 40%.

The state of the intestinal microbiota and antimicrobial protection: obtaining and analyzing information

Despite significant progress in the study of the qualitative and quantitative composition of microflora, there are many controversial issues related not only to diagnosis, but also to the treatment of dysbiotic disorders of the intestine. Dysbiotic disorders, characterized by a decrease in the qualitative and quantitative indicators of obligate and facultative microflora, can have a negative impact on human health. The doctor’s task is to restore the microbiocenosis of the gastrointestinal tract and the density of colonization of indigenous microflora, taking into account modern knowledge on this problem, presented in on-line training seminars.

БАКТЕРИОЛОГИЧЕСКАЯ ХАРАКТЕРИСТИКА ЭФФЕКТИВНОСТИ РАЗЛИЧНЫХ СПОСОБОВ ДРЕНИРОВАНИЯ СЛУЧАЙНЫХ РАН

The article presents data on the bacteriological control of the effectiveness of various methods of draining fresh accidental wounds with a wide area of damage in different species of animals: semipermeable membranes and tubular perforated drains. Semi-permeable cellulose membranes (experimental group) were inserted into the wound of animals with absolute indications for drainage at the final stage of surgical treatment.These membranes were filled with a hyperosmolar dialysis solution containing antibacterial preparations. The comparison group used the introduction of antiseptics through perforated tubular drains. In order to study the quantitative and qualitative composition of microflora in the wound process, bacteriological studies of swabbings from the wound surface and from the wound canal were performed on 1-3-7-9 days. As the result, it was established that the degree of tissue contamination depends on the duration of surgical debridement ,type and conditions of animals. The study of quantitative composition of micro flora in 1 g of tissues, in farm animals, with accidental wounds was up to 105 . The appliance of wound dialysis gave rapid destruction of associations of 2 and 3 types of microorganisms, in contrast to the control group of animals. The effect of applying membrane dialysis devices, in case of accidental wounds, allows to achieve positive results for 3-4 days. Bacteriological studies confirm the high antibacterial activity of dialysate, which was provided by the introduction of antibiotics and antiseptics into its composition. The introduction of antibiotics and antiseptics into the membrane drainage cavity once a day in the amount of a single therapeutic dose, allows for 7.1±1.3 days (P>0.001) to suppress the microflora in the wound. Higher antiseptic effectiveness of dialysates from semipermeable membranes is connected with their ability to create a constant high concentration of antibacterial substances in the wound focus. The mechanism of therapeutic effect is in a gradual and constant flow of antiseptics into the paravulnar tissues, by diffusion. This improves the conditions for the development of the body’s own phylactic power against infection and raises regenerative processes in the wound.

Assessment of antibacterial effectiveness of ultrasonic scaling in combination with calcium hydroxyappatite suspension among patients with type-1 diabetes

Relevance. Type-1 diabetes mellitus affects usual oral microbiome for active growth of periodontal pathogenic bacteria concentration in geometric progression, which leads to decrease in function of local immunity of oral cavity, inflammation of periodontal tissues and resorption of alveolar bone – the characteristics of chronic generalized periodontitis. This article provides analysis of qualitative and quantitative composition of microflora of periodontal pocket among type-1 diabetes patients before and after conservative periodontal therapy using ultrasonic treatment technique with a polishing suspension based on calcium hydroxide («Vector Paro», Durr Dental).Purpose. To evaluate the antibacterial effectiveness of professional oral hygiene complex with the additional treatment of periodontal pockets with «Vector Paro» device among patients with type-1 diabetes and chronic generalized mild periodontitis.Materials and methods. 30 patients with chronic generalized mild periodontitis and type-1 diabetes were examined with using analyses of periodontal pockets microflora contents. All patients underwent a standard complex of professional oral hygiene and gentle periodontal therapy with the «Vector Paro» device. Re-examination was in 4 weeks.Results. The performed complex of conservative periodontal therapy improved periodontal status in both groups, but in control group changes were more significant.Conclusion. Dynamic monitoring of qualitative and quantitative composition of periodontal pockets in combination with conservative periodontal therapy using the «Vector Paro» apparatus improves hygienic and periodontal status among patients with type-1 diabetes.

Experience of investigation the microflora and ejaculate proteins with different ultrasound picture of prostate

At present, there are practically no studies deciphering the mechanisms of the possible influence of opportunistic pathogens on the morphological changes in the prostate gland. Many authors doubt that bacteria are the cause of chronic prostatitis. In clinical practice with prostate diseases preference is given to ultrasound examination as a reliable and time-consuming diagnostic test. At the same time, many authors note that for the diagnosis of inflammatory diseases of reproductive organs, the study of relevant secrets is an actual and promising scientific direction.The aim of the study was the investigation of qualitative and quantitative composition of microflora, a number of ejaculate proteins against the background of a different echoscopic picture of prostate.Materials and methods. The study included 18 men was in infertile couples for more than 3 years with a previously diagnosed chronic prostatitis (N41.1, observation group), and 28 healthy volunteers (comparison group). All participants in the study performed transrectal ultrasound. In the ejaculate, the concentrations of immunoglobulins of the main classes, total protein and albumin, as well as the level of oxidative modification of proteins were determined. Microbiological examination of the ejaculate was performed according to generally accepted methods. For statistical evaluation of the data obtained, an unpaired version of Student's t-test was used. Differences were considered statistically significant at p0.05.Results. Microflora of the ejaculate in observation group was represented mainly by gram-positive cocci. In 39% of cases, the growth of Gram-negative microflora was established, from which Escherichia coli was isolated in 71% of the samples. In most samples, microorganisms were in the association, which is more typical for diffuse changes in prostate tissue. In cases with fibrosis, a noticeable narrowing of the spectrum of the released microflora was recorded: from Gram-negative bacteria only E. coli was isolated, and Gram-positive microorganisms were absent altogether. It was shown that the development of fibrosis in the prostate gland tissue is accompanied by an increase in the concentration of total protein and albumin in the ejaculate. When the process goes to the calcification stage, the levels of these indicators decrease. It was found that in all cases, with the exception of severe fibrosis, the concentration of IgG increases and IgA decreases. The development of fibrosis is accompanied by a decrease in the level of IgG. In the present study, in determining the level of oxidative modification of proteins, a decrease in the concentration of such molecules was found in patients of observation group.Discussion. In general, chronic inflammation in prostate of men of reproductive age in more than 60% of cases was characterized by fibrosis of the organ tissue and/or the formation of calcifications. In such situation, conditions are created for the persistence of opportunistic pathogenic microflora in fibro-modified tissue beyond the reach of immune system factors. In addition, there are phenomena of secondary immunodeficiency at the local level, manifested in a decrease in the number of leukocytes and their radical-producing function. That is why we consider the detection of conditionally pathogenic microorganisms as an essential fact of the pathogenesis of prostatitis, especially in the case of fibrosis and the formation of calcifications. Thus, the qualitative and quantitative study of microflora, the presence and degree of modification of the main protein components of the ejaculate, to a greater or lesser extent reacting to infectious inflammation, is primarily an asymptomatic one, broadens the concept of the pathogenesis of chronic prostatitis. As an addition to the ultrasound, its results allow us to clarify the duration of the inflammatory process, and to significantly decipher the echographic changes recorded at different stages of the development of the disease.

МОНИТОРИНГ МИКРОБИОЛОГИЧЕСКОЙ БЕЗОПАСНОСТИ И УСТАНОВЛЕНИЕ КОРРЕКТИРУЮЩИХ МЕР ПРИ ВЕТЕРИНАРНО-САНИТАРНОЙ ОЦЕНКЕ МЯСНОЙ ПРОДУКЦИИ В ООО «МОРОЗКО»

Currently, an urgent task for food enterprises of the Russian Federation is to improve the quality and safety of their products through the introduction of a system for analyzing critical control points (HACCP). This is important not only for the health of the population, but also for enhancing the advantages of products. The article presents materials concerning implementation of the principles of HASSP on the enterprise JSC «MOROZKO». Theoretical aspects of system of HASSP are given, problems are analyzed and recommendations about their minimization in the conditions of implementation of technical regulations of the Customs union 021/2011 «About safety of food products» are developed. Data of sanitary and microbiological control at veterinary and sanitary examination of the cooled and frozen meat of different software suppliers are provided to the normalized indicators in compliance with normative requirements of meat and meat products. Need of carrying out monitoring of microbiological indicators for critical control points is proved. On the basis of monitoring of microbiological indicators of raw materials, water, air, the equipment, overalls, hands of workers the assessment of risk of microbial contamination of finished goods by pathogenic microorganisms and microorganisms - activators of damage of raw materials and products is carried out. The quantitative composition of microflora was determined during air monitoring in the workshops of the enterprise. Comparative analysis of the influence of disinfection of different types of the equipment on its sanitary states by washout method from the equipment is carried out.

Obtaining and application of increased food and biological value iodinated products from lentils sprouted grain

The choice of research direction is related to the actual problem of production and distribution of functional purpose food products due to the spread of nutritional diseases and the lack of micronutrients in ordinary people and athletes diet. As an object for enrichment with iodine, it was suggested to use lentils, which is famous for its high protein content, low lipid and oligosaccharide content, and low inhibitory effect. The iodine accumulation occurs during germination, due to the use of a nutrient solution of the iodine inorganic form. In addition, the biochemical composition of the grain and the biological value of lentils are significantly improved: an increase in the content of total amino acids and vitamins is found to be 1.5-2.0 times, a mass fraction of the oligosaccharide fraction is observed. To determine the effect of technological processing on the degree of iodine conservation in lentils the grains were exposed to the following impact: grinding, extrusion, frying. An insignificant decrease in the amount of iodine during extrusion was noted and more significant one - during grinding. The obtained results of the determination of biological safety by the method of studying the effect of the investigated product on the growth response of ciliates allowed to confirm the safety of both fresh and dried sprouted grain of lentils. When studying the microbiology of grain by sowing on agarized selective diagnostic environments with subsequent identification of the qualitative and quantitative composition of microflora, including colony-forming units, deviations from the normative indices were not revealed. Experimental production of the extrudate was carried out, possible ways of its use in meat systems for improving the functional and technological properties of minced meat, as well as for independent use as snacks for the nutrition of athletes were suggested.

PREBIOTIC CORRECTION IN BACTERIAL VAGINOSIS

Increased interest to the vaginal microecosystems is largely due to the fact that the role of evolutionary formed microbial populations in this habitat lies not only in the maintenance of vaginal colonization resistance, but also in formation of microecological health of newborns, and consequently, the human population in general.The formation of the qualitative and quantitative composition of microflora is regulated by a complex mechanism of microbial interactions inside each of microecosystems and is controlled by physiological factors of the host organism in the dynamics of his life.

Structure and Activity of Microbial Community at Dung Aerobiotic Processing for Biofertilizer

Practicability of aerobiotic microbiological dung fermentation at poultry farms for production of biofertilizer with multi-functional action spectrum and solving of environmental issues in the field of industrial poultry farming is shown. Ways of the fermentation processes optimization are discussed, qualitative and quantitative composition of microflora during organic matter decay are analyzed.

Effect of Crops on the Humus Accumulation Process in the Grey Forest Soils of Priangarye

The influence of a number of agricultural plants in the crop rotation on the grey forest soils in the Irkutsk Region upon the intensity of the humification process was investigated by means of microbiological and biochemical tests. The synthesis, decomposition, and the accumulation ratio of humus were concluded from the phenoloxidase activity. The cellulose-decomposing microflora and algaeflora were taken into consideration as well. The humus synthesis processes were most active in the wheat and lucerne plots, they were less effective in the fallow and virgin soils. The lack of organic matter in the permanent fallow soil cuts down the humus accumulation ratio and the counts of microorganisms. The soil cultivation intensifies the humus synthesis processes and changes the composition of microorganisms in the soil. Algae which are mainly represented by green and blue-green species are an additional source of organic substance in the soil. Decomposition of organic substances in the soil proceeds with an active participation of cellulose decomposers which are mainly represented by fungal and bacterial flora. In rare cases actinomycetes can be found. Application of mineral fertilizers intensifies the humus accumulation process and improves the qualitative and quantitative composition of microflora in all the plots under investigation.