Quantification Of Phytochemicals Research Articles

Phytochemical quantification and optimization of synergistic antioxidant potency of Moroccan medicinal plants using constrained mixture design

Phytochemical quantification and optimization of synergistic antioxidant potency of Moroccan medicinal plants using constrained mixture design

Techno-functional characteristics, mineral composition and antioxidant potential of dietary fiber extracted by sonication from different oat cultivars (Avena sativa)

Metabolic disorders including hypertension, diabetes and cardiovascular disease etc. are the major threats to public health and economy. Management and prevention of alarmingly increased aforementioned disorders has attracted researchers to explore their natural remedies. The objective of this study was to explore ultrasonically extracted dietary fibers from indigenous oat cultivars (SGD2011, Avon, SGD81, PD2LV65, and S2000) for their techno-functional characteristics, mineral composition and phytochemicals. These characteristics will find the way to ultimately use oats in food and feed industries to address consumer demand. Extraction of dietary fibers from oat cultivars was optimized by response surface methodology. These cultivars varied significantly (p < 0.05) for oil holding capacity, water saturation and water holding capacity. The SGD81 cultivar showed the highest oil holding capacity, water holding capacity and water saturation due to its highest fraction of dietary fiber. The highest values of total phenolic contents, total flavonoid contents, total flavonol contents, 2,2-Diphenyl-1-picrylhydrazyl radical scavenging activity, and anthocyanins were shown by SGD81, and SGD2011. All cultivars varied significantly (p < 0.05) with respect to phytochemical quantification. The cultivars SGD81 and SGD2011 showed the best phenolic acid profile and can be effectively used as source of nutraceutical. Beyond well-established nutritional properties of oat, these may also contribute dietary fibers for their nutraceutical properties. This approach can protect people from metabolic disorders. Moreover, extraction of dietary fibers through sonication can be considered an eco-friendly strategy.

Unveiling the phytochemical profiles, selective bioactivity potential, and molecular docking study of bioactive compounds with target proteins using optimized bark extracts of Grewia asiatica L.

The current study aimed to explore the phytochemicals, estimate the anti-inflammatory, antioxidant, antimicrobial, and anticancer potentials of polyphenol enriched methanolic bark extract of Grewia asiatica (PEMGa), and perform molecular docking studies with identified phytocompounds. Here, we utilized response surface methodology (RSM) with a Box Behnken design (BBD) to enhance the phenolic content of the methanolic extract from G. asiatica bark by optimizing three crucial parameters like extraction time (h), temperature of extraction (°C), and solvent ratio (% v/v of methanol: water). Phytochemical quantification of PEMGa illustrated that the bark part contains a good amount of therapeutically potent phytochemical groups like phenolics, flavonoids, and tannins. Additionally, through HPLC analysis, eight compounds belonging to the phenolic and flavonoid groups have been quantified from PEMGa. By GC-MS (Gas chromatography–mass spectrometry) analysis of PEMGa, eleven phytochemicals were identified. Moreover, employing in-vitro bioactivity studies, PEMGa has unveiled its remarkable potential as an anti-inflammatory, antimicrobial, antioxidant, and anticancer agent. During the in-silico molecular docking study, out of eleven phytocompounds identified by the GC-MS study, three compounds, namely, γ-sitosterol, stigmasterol, and campesterol, formed the most stable docked complex with anti-inflammatory, antimicrobial, and antioxidant target proteins. The present study provides justification for the bark part of G. asiatica as a rich source of potent phytochemicals, and its potential against oxidative stresses, inflammations, cancer, and microbial infections.

Non-destructive optical indices to estimate isoprenoids with nutritional value in packed rocket and spinach

The quantification of phytochemicals with nutritional benefits relies on accuracy yet time-consuming, expensive and destructive methodologies. In contrast, optical indices widely employed in disciplines such as ecology can serve as fast, low-cost, and non-destructive tools for tracking pigment changes. However, their potential application in postharvest phases and commercialization (i.e., supermarkets) remains underexplored. In this context, this work aimed to investigate the feasibility of using the normalized difference vegetation index (NDVI), the chlorophyll content index (CCI) and the photochemical reflectance index (PRI) to estimate phytochemicals of nutraceutical interest (chlorophylls, carotenoids and tocopherols). A fully factorial and randomized experimental design (combining different illumination, packaging, and collecting time) was conducted in two green leafy packaged vegetables (rocket and spinach). With this design that induced differences in physiological parameters a database with variable content of isoprenoids (carotenoids, chlorophylls, and tocopherols) based on treatments was created. By establishing correlations and models between isoprenoids and optical indices, the NDVI was revealed as a valuable tool to estimate chlorophyll content in both species, while the PRI tracked both tocopherol and carotenoid (except for β-carotene) content. Taking this approach, estimation of the sum of total carotenoids and tocopherols was achieved using the PRI. This study confirms the possibility of extending the use of the NDVI and PRI to real-time nutritional monitoring in different stages of production and commercialization. A future application envisions integrating this technology into refrigerators for enhanced consumer access to nutritional information.

African pear (Dacryodes edulis) pulp antioxidants and bioactive compounds: Outcome of heat treatment

The study was aimed at evaluating the effect of heat treatment (boiling and roasting) on the antioxidant properties and phytochemical profile of African pear pulp. African pear was subjected to boiling and roasting treatment at 100 °C for 5 mins and 150 °C for 10 mins respectively. The raw, boiled, and roasted pears were analysed for antioxidants and phytochemicals were profiled using HPLC. The results of antioxidants showed that total phenols (mg GAE/g) decreased by 34 % in the boiled pears and by 18 % in the roasted pears; total flavonoids (Rutin equivalent) decreased by 50 % in the boiled pear while it was not significantly affected by roasting; DPPH radical scavenging abilities decreased from 84.15 % to 50.82 % and from 84.15 % to 57.04 % in the boiled and roasted pears. Beta-carotene was found to decrease by 17 % and 64 % in the boiled and roasted pears. HPLC quantification of phytochemicals revealed Beta Caryophyllene as the most abundant in the raw and heat-treated pears though heat treatment increased all the phytochemicals quantified. Roasting was found to significantly increase all the bioactive compounds quantified. This suggests that the roasted pear will contribute to better nutrition and health security when consumed compared to the boiled pear.

Influence and distribution of lead nitrate on growth and secondary metabolite accumulation in Withania somnifera (L.) Dunal in vitro shoots

Withania somnifera is a medicinal ayurvedic plant native to India. Lead (Pb) is a toxic heavy metal which has no biological function in plants. The aim of the present study is to investigate the influence of heavy metal Pb on W. somnifera at cellular level under controlled conditions. The role of Pb on shoots of W. somnifera was determined in terms of biomass, secondary metabolite production (High Performance Thin Layer Chromatography) and phytochemical quantification. Influence and accumulation of Pb at the cellular level were recognized using UV visible spectroscopy, field emission scanning electron microscope and x-ray diffraction analysis. The current result showed that the concentration up to 2.4 mM Pb for 7 days of exposure showed higher biomass and withaferin A yield compared to control shoots. On the other hand, other secondary metabolites such as flavonoids, phytosterols and phenols levels were reduced compared to control shoots. In the morphological study, Pb concentration in the analysed sample was determined as 2%. The probability of the nanoparticle nature of the bioaccumulated lead also verified using spectroscopy and x-ray diffraction analysis. To date, we are the first to report on the influence of Pb on in vitro shoot cultures of W. somnifera.

Phytochemical Quantification and Characterization of Anti- Diabetic Potential of Polyherbal Formulation by FT-IR and GC-MS Analysis

The main objective of the present study was to develop and evaluate anti-diabetic potential of polyherbal formulation (PHF) using Nigella sativa, Cinnamomumverum, Allium sativum, Zingiberofficinale, Curcuma longa and Trigonellafoenumgraecum for management of diabetes. The PHF was investigated by advanced analytical techniques. The proximate analysis of PHF revealed all parameters were within the limits indicating no adulteration and contamination. In addition, gas chromatography-mass spectrometry (GC-MS) and Fourier transform-infrared (FT- IR) spectroscopy analysis showed the presence of bio-active phytochemicals including phenolic compounds, antioxidants, anti-inflammatory and anti-diabetic constituents that are good therapeutic potential for prevention and management of diabetes. The efficacy of PHF was evaluated by dividing into four groups (PHF 1.5 g, PHF 3.0 g, metformin 500 mg and placebo) of newly diagnosed type 2 diabetic patients for 90 consecutive days and monitored on a monthly basis. PHF 3.0 g dose showed a significantly higher anti-diabetic effect as compared to PHF 1.5 g while comparable results in relation to metformin 500 mg. The phytochemical characterization of PHF will ensure its quality and safety. Moreover, the anti-diabetic efficacy of PHF is comparable with anti-diabetic efficacy of metformin. PHF has the potential to achieve glycemic control in type 2 diabetic patients with a diabetic diet prescribed.

Qualitative and Quantitative Phytochemical profiling of Digera muricata (L.) Mart Leaves

Plants possess an extensive variety of therapeutic compounds that exhibit significant potential for application within the pharmaceutical industry. In order to identify the diverse phytochemical composition present in the plant, range of solvents including alcoholic, aqueous, hexane, petroleum ether and hydroalcoholic were employed these extracts were used and the preliminary qualitative analysis reveals the presence of phytochemicals in higher concentrations in the hydro ethanolic extract compared to other solvent. The powdered plant sample was further investigated for the quantification of phytochemicals which revealed the presence of high total phenolic content, total flavonoid content, saponin content, total tannin content and steroids content of 246.82 ± 17.27 GAE equivalents per gram, 193.18 ± 13.52 milligrams of quercetin equivalents per gram, 134.76 ± 9.43 milligrams of saponin equivalents per gram, of 61.80 ± 4.32 milligrams of Tannic acid equivalents per gram and 117.66 ± 8.23 milligrams of Cholesterol equivalents per gram respectively. The present study elucidates the potential for isolating specific compounds possessing economically valuable bioactive properties through the utilization of suitable plants and careful selection of an appropriate solvent for extraction.

Artemisia brevifolia Wall. Ex DC Enhances Cefixime Susceptibility by Reforming Antimicrobial Resistance.

(1) Background: A possible solution to antimicrobial resistance (AMR) is synergism with plants like Artemisia brevifolia Wall. ex DC. (2) Methods: Phytochemical quantification of extracts (n-hexane (NH), ethyl acetate (EA), methanol (M), and aqueous (Aq)) was performed using RP-HPLC and chromogenic assays. Extracts were screened against resistant clinical isolates via disc diffusion, broth dilution, the checkerboard method, time-kill, and protein quantification assays. (3) Results: M extract had the maximum phenolic (15.98 ± 0.1 μg GAE/mgE) and flavonoid contents (9.93 ± 0.5 μg QE/mgE). RP-HPLC displayed the maximum polyphenols in the M extract. Secondary metabolite determination showed M extract to have the highest glycosides, alkaloids, and tannins. Preliminary resistance profiling indicated that selected isolates were resistant to cefixime (MIC 20-40 µg/mL). Extracts showed moderate antibacterial activity (MIC 60-100 µg/mL). The checkerboard method revealed a total synergy between EA extract and cefixime with 10-fold reductions in cefixime dose against resistant P. aeruginosa and MRSA. Moreover, A. brevifolia extracts potentiated the antibacterial effect of cefixime after 6 and 9 h. The synergistic combination was non- to slightly hemolytic and could inhibit bacterial protein in addition to cefixime disrupting the cell wall, thus making it difficult for bacteria to survive. (4) Conclusion: A. brevifolia in combination with cefixime has the potential to inhibit AMR.

Quantification of Phytochemicals and Metal Ions as well as the Determination of Volatile Compounds, Antioxidant, Antimicrobial and Antacid Activities of the Mimosa pudica L. Leaf: Exploration of Neglected and Under-Utilized Part.

Mimosa pudica L. (MP) is well-known plant in traditional medicinal system, especially in India. Unfortunately, leaves of MP are less explored. To determine the food and nutritional value of the neglected part of Mimosa pudica L. (MP), that is MP leaves, phytochemicals and metal ions of MP were quantified by newly developed HPLC and ICPOES-based methods. The content of phytochemicals observed using HPLC analysis for chlorogenic acid, catechin, and epicatechin was 141.823 (±8.171), 666.621 (±11.432), and 293.175 (±12.743) μg/g, respectively. Using GC/MS/MS analysis, fatty acid like oleic acid were identified. In ICP-OES analysis, a significant content of Na, K, Ca, Cu, Fe, Mg, Mn, and Zn was observed. The observed TPC and TFC for MP leaf extracts was 44.327 (±1.041) mg GAE/ g of wt. and 214.217 (±4.372) mg QCE/ g of wt., respectively. The DPPH assay depicted a strong antioxidant activity of MP leaf extracts with IC50 values of 0.796 (±0.081) mg/mL and a TEAC value of 0.0356 (±0.0003). A significant antacid activity (666 mg MP+400 mg CaCO3 >400 mg CaCO3 ≫666 mg Gelusil) of MP leaves was noticed. The methanolic extract of MP leaves demonstrated anti-microbial activity against Staphylococcus aureus (15±2mm), Pseudomonas aeruginosa (12±2mm) and Escherichia coli (10±2mm). In silico studies confirmed the in vitro results obtained for antioxidant, antiacid, and anti-microbial activities. In addition, in silico studies revealed the anti-cancerous and anti-inflammatory potential of the MP leaves. In summary, this study demonstrated the medicinal significance of MP leaves and the conversion of agro-waste or the under-utilized part of MP into pharmaceutical potent materials. Consequently, the present study highlighted that MP leaves alone have medicinal importance with good nutritional utility and possess large promise in the pharma industry along with improving bio-valorization and the environment.

Estimation of phytochemical constituents and analysis of antioxidant activity in different parts of plant

Oxidative stress is defined as a condition in which the balance between the production of reactive oxygen species (ROS) and the antioxidant defense system gets disturbed and causes free radical induced diseases. The current research was aimed to carrying out quantification of phytochemicals in different parts of and explores the antioxidant activities of ethanolic extracts of leaf (CFL), seed (CFS), and bark (CFB) to show their therapeutic importance. The antioxidant potential of plant parts was assessed by free radical scavenging activity (FRSA), superoxide anion radical scavenging activity (SARSA), reducing power (RP), lipid peroxidation (LPO), hydroxyl radical scavenging activity (HRSA), and ferric thiocyanate activity (FTC). Quercetin was used as a standard to measure the antioxidant activity. Among the tested plant extracts, CFB had the highest total phenolics and protein content than those of CFS and CFL, whereas CFL had the highest ascorbic acid, carotenoids and carbohydrate content than CFB and CFS plant parts. Antioxidant assay showed CFB had the lowest ICvalue for FRSA (35.48 μg/ml), LPO (91.91 μg/ml), HRSA (106.87 μg/ml) and FTC (53.21 μg/ml). CFS showed the lowest IC for SARSA (24.54 μg/ml) and the highest RP (2.14 ASE/ml). Overall, the bark of could be a potential natural antioxidant source for food as well as pharmaceutical applications.

The Ethnobotanical Investigation, Phytochemistry and Antioxidant Activity of a Medicinal Plant Recipe Directed against Candida albicans and Enterobacteria Stains Producing ESBL CTX-M-15 Type in Burkina Faso

Aims: The objectives are to carry out an ethnobotanical survey among traditional healers in order to choose the most recipe used in the treatment of vaginal candidiasis in cities of Burkina Faso followed by phytochemical quantification, antioxidant activity and antimicrobial of the best recipe.&#x0D; Methodology: Ethnobotanical surveys were conducted in Bobo-Dioulasso and Dédougou by semi-structured interview amount traditional healers. The extracts were obtained by ethanolic and hydroethanolic maceration. Characterization of the secondary metabolites was revealed by the tests in tubes. Polyphenolic compounds contents quantification was done by spectrophotometry using Follin-Ciocalteu reagent, aluminum trichloride and vanilic acid. The antioxidant activity was evaluated by two methods: DPPH• and FRAP. The Minimal inhibitory concentration on Enterobacteria strains was determined by the dilution method and the minimal bactericidal concentration was determined by inoculation on agar. The susceptibility test of Candida albicans strains was carried out using the disc diffusion assay.&#x0D; Results: In total, 52 traditional healers were surveyed with a predominance of men (75%) in the both cities. 38 recipes of medicinal plants have been obtained. Decoction is the most frequent method of preparation in Bobo-Dioulasso (87.5%) and in Dédougou (27.27%). The leaves (44%) and the bark of the trunk (31.82%) are respectively the most parts used of the plants. Colorimetric tests revealed the presence of compounds such as flavonoids, alkaloids, tannins and saponosides. The best results of quantification tests of total phenolics, flavonoids and tannins, were obtained with the hydroethanolic extract (80.27±20.27(mgEAG/10 mg); 0.27±0.01(mgEQ/10 mg) and 73.31±4.65 (mgEC/10 mg)). With antioxidant activity, the highest value was obtained with the DPPH radical inhibition method (85.59±0.001%). Minimal inhibitory concentration revelead that the highest value was 0.781 mg/ml and the best minimal bactericidal concentration was 3.123 mg/ml.&#x0D; Conclusion: Phytochemical and biological analysis realized could be partially justified by the recipes used in the treatment of vaginal candidiasis.

Comparative bioactive compounds and fourier transform infrared spectroscopic evaluation of Azadirachta indica extracts and its potential as bio-fungicides against plant pathogenic fungi

New antifungal agrochemicals compounds discovering from natural products are a vital goal for the management of plant pathogenic fungi. As present synthetic fungicide used for the treatment of plant pathogenic fungi have negative effects on environment and human health. Therefore, it is need of the day to develop and discover new antifungal substances from natural products. The objective of the current study was to evaluate the extracts of leaves, seeds and twigs of Azadirachta indica using infusion, decoction and microwave extraction techniques. These extracts were subjected to phytochemical quantification, Fourier Transform Infrared (FTIR) spectroscopic analysis and antifungal assays against pathogenic fungi by well diffusion technique. The quantitative of phytochemicals screening showed that leaves had showed high concentrations of bioactive compounds, seeds showed a moderate and twigs observed the least phyto-compounds. The infusion extracts system showed high amount of active compounds, followed by microwave and decoction. Extracts of leaves and seeds showed evidence of inhibition growth of fungi. The spectra of FTIR verified the occurrence of different functional groups such as aromatic compounds, alkanes, alkyl halides, amines, carboxylic acids, phenols, alcohol and amino acids, in the neem extracts. Relevant stakeholders along with the help of policy makers and researchers should build extra understanding regarding the requirement to clinch products of organic fungicides like secure fungus management system.

Microscopic evaluation, ethnobotanical and phytochemical profiling of a traditional drug Viola odorata L. from Pakistan

Background: The present study encompassed a highly traded medicinal plant Viola odorata L. (Violaceae) for detailed Light and Scanning Electron Microscopy, ethnobotany and phytochemical evaluation. Phytochemical evaluation included ascorbic acid, nutritional, and phytochemical profile, and essential and fixed oil study. Even though each feature has its own limited taxonomic value but collectively these characteristics may be systematically important especially for the discrimination and identification of complex and problematic taxa. Methods: The aim was to study microscopy, histology and phytochemical composition of Viola odorata on the basis of ethnobotanical information cited in the literature. Methods: The microscopy, and phytochemical composition of V. odorata was studied using standard methods. Results: Anatomy of the plant parts depicted dicot histology. Stomatal study under LM and SEM, revealed the presence of diacytic and anisocytic type of stomata. Stomata were numerous on the lower epidermis of the leaf. SEM of the powder drug showed the presence of trichomes, calcium-oxalate crystals, pitted vessels, fibers, trichomes, pollen grains, parenchyma cells, pith cells and root hair, but some unknown tissues were also seen. Ascorbic acid, nutritional, and phytochemical profile was investigated according to the standard methods. Different parts of the plant contained various chemical constituents such as alkaloids, mucilage, anthraquninone, saponins, tannins, fats and oil, protein and starch. Quantification of phytochemicals revealed mucilage and tannins to be the highest as compared to saponins and alkaloids. Leaves had 0.00143 % essential oil and 0.396 % fixed oil. Ascorbic acid, nutritional, and phytochemical profile, and oil study revealed vitamin C, proximate and phytochemical composition of V. odorata. Conclusion: Overall, this study can be helpful for plant taxonomists to further analyze the species for phytochemical isolation. This will improve the regulatory process and reduce the risk of a quality breach. Keywords: Ethnobotany, Histology, Morphology, Phytochemical evaluation, SEM, Viola odorata L.

Quantification of phytochemicals, and compounds' identification in functional tea from Ficus capensis and Justicia secunda.

This work evaluated the development of beverage from Ficus capensis and Justicia secunda leaves composite using response surface methodology three-level factorial design gas chromatography-mass spectrophotometry. Fresh leaves of F. capensis and J. secunda were processed into tea. The results indicated the optimal extracts blends of 48% of F. capensis and 52% of J. secunda. The iron content was 489.11mg/g; flavonoid content, 83.64mg QE/100g; vitamin C content, 1003.14mg/100g; and pyridoxine content, 357.31mg/100g. Alkaloid profile showed tetrahydrorhombiofoline (35%), columbamine (20.10%), laudanosine (11.50%), salsoline (8.22%), methylcobalamin (7.98%), quinine (5.89%), and quinidine (3.21%). Other phytocompounds with bioactive characteristics include 11-octadecenoic acid, methyl ester (32.43g/100g); hexadecanoic acid, methyl ester (16.69g/100g); 9,12-octadecadienoic acid (Z,Z)-, methyl ester (12.81g/100g); (E)-9-octadecenoic acid, ethyl ester (9.71g/100g); hexadecanoic acid, ethyl ester (5.86g/100g); methyl stearate (4.62); linoleic acid, ethyl ester (4.62g/100g), octadecanoic acid, ethyl ester (1.84g/100g); and octasiloxane, 1,1,3,3,5,5,7,7,9,9,11,11,13,13,15,15-hexadecamethyl (0.19g/100g) with good sensory attributes. The optimized tea from F. capensis and J. secunda is rich in health-supporting substances and could be employed in promoting healthy living.

Physicochemical, antioxidant and antimicrobial activities of green teas manufactured from common tea clones of different gardens in Bangladesh

Green tea's health-promoting actions are mostly due to its phytochemical content and antioxidant property. Due to the toxicity and cancer-causing properties, synthetic antioxidants are injurious to human health. Therefore, natural products such as teas might be chosen as antioxidants and antibacterial agents instead of synthetic ones. This investigation was performed to analyze the phytochemical constituents, antioxidant and antibacterial characteristics of green teas produced from some commonly cultivated tea clones of different gardens in Bangladesh. pH, total soluble solids, vitamin B (B 1 , B 2 ) and C, caffeine and tannin of green teas were estimated for the quantification of phytochemicals. DPPH free radical scavenging, total phenolic content, and total flavonoid content were quantified to determine antioxidant activity. Gram-positive Bacillus spp. and Gram-negative Escherichia coli were used for antibacterial activity. BT 2 revealed the greatest level of caffeine and tannin content of 2.033 and 4.846 g/100g sample respectively. TV 9 showed the highest amount of vitamin B 1 , B 2 , and C which were 1450.02, 2707.12, and 2983.04 mg/100g sample respectively. TV 9 also exhibited the highest antioxidant capacity of 78.23%, the highest amount of phenolics (2.25 g GAE/100g sample), and the highest levels of flavonoids (918.27 mg QE/100g sample). In the case of antimicrobial activity, TV 9 exhibited the highest zone of inhibition (ZOI) of 15.61 mm against Escherichia coli which was moderately susceptible. BT 2 showed the highest ZOI of 17.35 mm against Bacillus spp. which was susceptible. These tea extracts can be employed in medicine and food sector as an alternative to synthetic antioxidants and antibacterial agents. • Green tea's health-promoting actions are due to its phytochemical content. • BT 2 exhibited the maximum level of caffeine and tannin content. • TV 9 showed highest vitamin B 1 , B 2 , C, antioxidant and antimicrobial activity. • These can be used in medicine and food sector as an alternative to synthetic agent.

Biochemical, nutraceutical and phytochemical characterization of chia and basil seeds: A comparative study

ABSTRACT Chia and basil seeds have comparable nutritional profiles, bioactive components, and phytochemistry, which make it difficult for consumers and researchers to choose between the two. Thus, an in-depth study was conducted to compare the physiochemical, nutritional and bioactive profile of these seeds in order to obtain complete database regarding nutritional and phytochemical profiling of both seeds. The wonder seeds were analyzed for physico-chemical composition, fatty acid constituents, amino-acid profile and phytochemical screening. Fatty acid constituents were determined using GC-MS, while amino acids profile and identification and quantification of phytochemicals were performed using HPLC procedure. Physico-chemical evaluation of basil and chia seed revealed that basil was found superior to chia in terms of dietary fiber (40.85 g/100 g), fat content (33.10 g/100 g), vitamin A (1583 µg/100 g) and E (779 µg/100 g) while chia seed was rich in protein (21.54 g/100 g), iron (7.7mg/100 g), magnesium (335 mg/100 g) and phosphorus (860 mg/100 g). Amino acid profiling revealed the presence of high-quality protein in chia seeds comprising of almost all essential and non-essential amino acids in significant (p < .05) amounts as compared to basil seeds. Furthermore, higher proportion of α-linoleic acid (ALA) was detected in basil seeds (24.4g/100 g) as compared to chia seeds (5.25 g/100 g) as determined by GC-MS. Phytochemical screening showed that basil contained significantly (p < .05) higher amounts of total phenolic (17.66mgGAE/g of dry sample) and flavonoid content (0.57mgQE/100 g of dry sample) in comparison to chia seeds, which contributed to its higher anti-oxidant activity. Phenolic characterization revealed that basil seeds contained higher concentration of caffeic acid (4780.01 µg/g) followed by gallic acid (2330 µg/g). The inference drawn from the study concludes that both seeds can be put forth for enrichment of various food items or can be directly consumed as functional foods.

Determination and Quantification of Phytochemicals from the Leaf Extract of Parthenium hysterophorus L. and Their Physio-Biochemical Responses to Several Crop and Weed Species

This current investigation was undertaken both in laboratory and glasshouse for documentation and quantification of phytochemicals from different parts of the parthenium (Parthenium hysterophorus L.) plant through LC-MS and HPLC to study their effect on two crops namely, Bambara groundnut (Vigna subterranean L.) and maize (Zea mays L.), and six different types of weed e.g., Digitaria sanguinalis, Eleusine indica, Ageratum conyzoides, Cyperus iria, Euphorbia hirta, and Cyperus difformis. The parthenium methanolic leaf extracts at 25, 50, 75, and 100 g L-1 were sprayed in the test crops and weeds to assess their physiological and biochemical reactions after 6, 24, 48, and 72 h of spraying these compounds (HAS). The LC-MS analysis confirmed seven types of phytochemicals (caffeic acid, ferulic acid, vanillic acid, parthenin, chlorogenic acid, quinic acid, and p-anisic acid) in the parthenium leaf extract that were responsible for the inhibition of tested crops and weeds. From the HPLC analysis, higher amounts in leaf methanol extracts (40,752.52 ppm) than those of the stem (2664.09 ppm) and flower extracts (30,454.33 ppm) were recorded. Parthenium leaf extract at 100 g L-1 had observed higher phytotoxicity on all weed species except C. difformis. However, all crops were found safe under this dose of extraction. Although both crops were also affected to some extent, they could recover from the stress after a few days. The photosynthetic rate, transpiration rate, stomatal conductance, carotenoid and chlorophyll content were decreased due to the application of parthenium leaf extract. However, when parthenium leaf extract was applied at 100 g L-1 for 72 h, the malondialdehyde (MDA) and proline content were increased in all weeds. Enzymatic antioxidant activity (e.g., superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) contents) were also elevated as a result of the sprayed parthenium leaf extract. The negative impact of physiological and biochemical responses as a consequence of the parthenium leaf extract led the weed species to be stressed and finally killed. The current findings show the feasibility of developing bioherbicide from the methanolic extract of parthenium leaf for controlling weeds, which will be cost-effective, sustainable, and environment friendly for crop production during the future changing climate.

Antioxidant, Anti-Cancer Activity and Phytochemicals Profiling of Kigelia pinnata Fruits

Over the past few years, research studies on the therapeutic benefits of medicinal plants with potent antioxidant activity and few side effects have grown significantly. This has sparked interest in determining whether naturally occurring antioxidants could take the place of synthetic antioxidants, which are currently being constricted because of their toxic and carcinogenic properties. The identification and quantification of phytochemicals in the methanolic extract of Kigelia pinnata fruits was measured using gas chromatography–mass spectrometry (GC-MS) and ultra-high-performance liquid chromatography–mass spectrometry (UPLC-MS/MS) techniques. Additionally, the methanolic extract of fruits was used to determine antioxidant activity. Free radical-scavenging (DPPH) and ferric ion-reducing antioxidant power were measured using spectrophotometry, and total antioxidant capacity (TAC) was compared with two common antioxidants, vitamin C and α-tocopherol. Moreover, mature fruits have high DDPH, ferric ion-reducing antioxidant power and total antioxidant capacity. Furthermore, mature fruits have high levels of total phenolic, flavonoid, and tannin content; these compounds are thought to be the sources of the antioxidant activity. The major constituents of the methanolic extracts from the mature fruits of K. pinnata were found to be larixinic acid, 3,5-Dihydroxy-6-methyl-2,3-dihydro-4H-pyran-4-one (DMDP), and 5-Hydrxoymethylfurfural. We performed the elemental analysis of the whole fruit. Huh-7 (liver cancer), PANC-1 (pancreatic cancer), Colo-205 (colorectal cancer), HT-29 (colorectal cancer), SNU-16 (gastric carcinoma), SW620 (colorectal adenocarcinoma) and HCT116 (colon carcinoma) were tested in vitro for anticancer activity. Both methanolic and ethyl acetate extracts of mature fruits had a positive effect on all cancer cell lines as compared to the doxorubicin drug. In addition, the methanolic extracts of mature fruits showed more potent cytotoxic effects than the ethyl acetate extracts. Moreover, the most pronounced cytotoxic effects of the methanolic extract were detected in SW620 (colorectal adenocarcinoma), with an IC50 value of 6.79 μg/mL, SNU-16 (gastric carcinoma), with and IC50 value of 8.69 μg/ ml, and in PANC-1 (pancreatic cancer) with an IC50 value of 10.34 μg/mL. Moreover, the results show that the water, ethyl acetate and methanolic extracts of mature fruits have antioxidant capacity, ferric ion-reducing antioxidant power, DPPH scavenging activity and also anticancer activity. Therefore, the present study suggests that the phytochemical profiles of mature fruits of K. pinnata may be used as potential natural antioxidants and anti-cancer cell lines.

Phytochemical Screening, Total Flavonoid, Phenolic content assays and Antioxidant activity of selected Unani Formulations

In the present study, unani formulations Habb-e-Suranjan, Habb-e-Azaraqui are evaluated for the presence and quantification of phytochemicals, total antioxidant capacity and ferric reducing antioxidant capacity. The total phenolic and flavonoid content were estimated according to folin-ciocalteau and aluminum chloride colorimetric methods respectively. The total antioxidant capacity and ferric reducing antioxidant capacity were conducted according to standard methods. The results of phytochemical screening revealed the presence of phenols, flavonoids, saponins, phytosterols and tannins in Habb-e-Suranjan and Habb-e-Azaraqui. The quantitative study of total phenols and flavonoids in these formulations were expressed in terms of Gallic acid and Quercetin equivalents. The total antioxidant capacities of the formulations were less compared to standard ascorbic acid. Hence concluded that the studied unani formulations shown to possess phyto-therapeutic constituents which may be responsible for antioxidant and reducing power activities. Overall, the formulations are proving the claimed therapeutic activities by antioxidant mechanism.