Quantification Of Nicotine Research Articles

Online quantification of nicotine in e-cigarette aerosols by vacuum ultraviolet photoionization mass spectrometry.

The growing popularity of e-cigarettes and the associated risks of nicotine addiction present a new challenge to global public health security. Measuring the nicotine levels in e-cigarette aerosols is essential to assess the safety of e-cigarettes. In this study, a rapid in situ method was developed for online quantification of nicotine in e-cigarette aerosols by using a homemade vacuum ultraviolet photoionization aerosol mass spectrometer (VUV-AMS). E-cigarette liquids with different nicotine concentrations were prepared to generate aerosols containing different levels of nicotine, which were employed as the calibration sources for nicotine quantification by VUV-AMS. The results showed that the mass concentration of nicotine in e-cigarette aerosols has a good linear relationship with its signal intensity in the mass spectrum, and the limits of detection and quantitation of nicotine by VUV-AMS were found to be 2.0 and 6.2 μg per puff respectively. Then the online method was utilized to measure five commercial e-cigarettes, and their nicotine yields were determined to be between 31 and 188 μg per puff with the nicotine fluxes from 7.7 to 70 μg s-1, agreeing with the results of the gas chromatography with a flame ionization detector (GC-FID). This study demonstrated the feasibility and advantages of VUV-AMS for quick quantification of nicotine in e-cigarette aerosols within seconds.

Chromatography and Spectroscopy Methods for the Analysis of Nicotine and Other Chemical Ingredients in E-Liquid Formulation: A Review

Spectroscopy and chromatography methods have become the most preferred techniques for detecting ingredients in e-liquid analysis. Both methods are widely used for separating and quantifying volatile compounds in a sample, providing individual chemical information in complex mixtures. This paper aims to review the detection and quantification of nicotine and other chemical ingredients in e-liquid used in e-cigarettes. E-cigarettes use the evaporation-condensation principle of aerosolization to produce an inhaled vapor containing nicotine, excipients, and flavoring agents. This review covers sample preparation, identification, and quantification of nicotine and other ingredients using chromatography and spectroscopy analysis. The spectroscopy methods are useful for quickly identifying and quantifying volatile compounds, including propylene glycol (PG), vegetable glycol (VG), and nicotine, while spectroscopic methods, particularly the Attenuated Total Reflectance-Fourier Transform Infrared Spectroscopy (ATR-FTIR) method, have lower analytical performance compared to chromatography methods in detecting nicotine and other chemical ingredients. Based on the review, chromatographic methods are the primary option for analyzing nicotine in all e-liquid samples, offering a better alternative for a future study with the presence of multiple detectors.

Investigating the efficiency of mechanical agitation on the quantification of nicotine in e-cigarettes, using a novel method

Electronic cigarettes (ECs) have gained popularity among the general public. ECs have varying nicotine levels from one e-liquid to another. Thus, a standardised method for analysing the nicotine content in e-liquids is needed. Here, a method was developed for quantitative analysis using high-performance liquid chromatography (HPLC) with mechanical agitation (MA). The same elution conditions were applied without mechanical agitation (WMA) to investigate the effect of processing on nicotine separation efficiency. The proposed method resulted in increased linearity in a concentration range of 25–500 ppm. This method also resulted in an increase in the correlation coefficient from 0.2415 to 0.9991, with a limit of detection (LOD) and limit of quantification (LOQ) of 0.09 ppm and 0.29 ppm, respectively, making it more applicable to a wide range of e-liquids in domestic and foreign markets. The analysis of seven e-liquids with concentrations of 0–50 mg/mL revealed that 67% of the samples had a lower nicotine content than that stated on the label. The opposite was true for 33% of samples. No sample matched the nicotine content stated on the label. A sample that was claimed to contain 3 mg/mL actually contained 17 mg/mL. Moreover, a sample labelled as nicotine-free contained 7 mg/mL of nicotine. Another sample was claimed to contain 50 mg/mL, but the measured value was 24 mg/mL. This value exceeds the Saudi Food and Drug Administration (SFDA) standard, which specifies that e-liquids should not contain more than 20 mg/mL of nicotine.

Evaluation & Comparison of Nicotine quantification in smokeless tobacco products

Introduction: Smokeless tobacco products (STPS) represent a significant health risk and have been associated with oral and pancreatic cancers, oral lesions, coronary artery and peripheral vascular disease and adverse pregnancy outcomes. So, the aim of the present study is to perform quantitative determination of nicotine, the main alkaloid of smokeless product (Gutkha) available in Vadodara, Gujarat. Method: Collection of sample was done from local tobacco selling shopkeeper from Vadodara i.e. vimal, RMD, pan vilas, rajnigandha and raag. All the samples included in the study the same products available everywhere in Gujarat. The quantification of nicotine was done by High Performance Thin Layer Chromatography (HPTLC) using the mobile phase toluene: ethyl acetate: Diethylamine (6:4:0.5). Spectrodensitometric measurement was carried out at absorption maximum 254 nm. Results: Five different smokeless tobacco samples were estimated using HPTLC method. Nicotine content was found to be 2.45% in Vimal, 3.11% in RMD, 2.60%- Pan Vilas, 3.06%- Rajigandha,3.32%- Raag. Conclusion: A considerable variation of nicotine content was found among the five investigated smokeless tobacco product where sample raag revealed the highest amount of nicotine than the other samples. The nicotine concentration of commercially available chewing tobacco products was found to be much lower than that of the smoking form of tobacco, but the higher average daily consumption made it comparable to the smoking form.

Simple HPLC method for simultaneous quantification of nicotine and cotinine levels in rat plasma after exposure to two different tobacco products

AbstractPurposeDevelopment and validation of a selective analytical method to accurately and precisely quantify nicotine and cotinine levels in rat's plasma after exposure to tobacco cigarettes and tobacco water-pipe.MethodsAn easy HPLC-Photodiode-Array Detection (PDA) method was developed and validated for simultaneous determination of nicotine and cotinine levels in plasma of 15 rats (10 rats after tobacco products exposure and 5 control rats). Nicotine and cotinine were extracted in one step from plasma using acetonitrile and concentrated to lowest volume using nitrogen stream.ResultsThe developed method offered a rapid analysis time of 14 min with single step of analytes extraction from rat's plasma with recovery percentage range between 93 and 95% and excellent linearity with correlation factor more than 0.994 with analytical range between 50 and 1000 ng mL−1 and LOD of 25 ng mL−1 and 23 ng mL−1 for nicotine and cotinine, respectively. The analysis of rat's plasma after 28 days of exposure to tobacco cigarettes and tobacco water-pipe revealed that the average concentrations of 376 ng mL−1 for cotinine and 223 ng mL−1 for nicotine were obtained after tobacco cigarettes exposure, and 220 ng mL−1 for cotinine and 192 ng mL−1 for nicotine after tobacco water-pipe exposure.ConclusionHigher nicotine and cotinine levels were found in plasma after tobacco cigarettes exposure than water-pipe exposure which may have potential undesirable effects on passive smokers in both cases.

Chemical analysis of electronic cigarette liquids (e-liquids) and direct nicotine quantitation using surface-assisted flowing atmospheric-pressure afterglow desorption/ionization mass spectrometry (SA-FAPA-MS).

Ambient desorption/ionization mass spectrometry (ADI-MS) has been widely used for direct analysis of real samples without sample preparation or separation. Studies on the quantification of low molecular weight compounds in complex matrices with ADI-MS remain scarce. In this paper, we report the application of surface-assisted flowing atmospheric-pressure afterglow mass spectrometry (SA-FAPA-MS) for fast qualitative screening of electronic cigarette liquid (e-liquids) ingredients and direct quantification of nicotine. The quantification approach is rapid, uses a deuterated D4-nicotine standard spike, and does not require a preceding chromatography step or other methods to remove the complex sample matrix. Selected e-liquids were directly applied on thin-layer chromatography (TLC) plate surfaces (normal phase (NP) silica, reversed phase (RP) modified silica, cyano (CN) modified silica, and dimethyl (RP2) modified silica) after dilution and internal standard spiking. The plates served purely as sample carriers and no analyte separation was performed. Promising qualitative results were obtained, demonstrating the ability to detect nicotine alkaloids using this approach and the ability to differentiate e-liquids based on their flavor variations. In addition, dimethyl- (RP2-) and cyano-modified (CN-) silica surfaces were selected for quantification based on performance results of previous studies. It was shown that results were in high accordance with high-performance liquid chromatography (HPLC) experiments with lowest deviations <3% on dimethyl surfaces. Additional quantitative experiments including a certified reference material achieved equally satisfying results with lowest deviations of -1.1% from the certified nicotine content. For nicotine, detection limits down to the fmol range (96 fmol on CN and 20 fmol on RP2) were obtained. A detailed comparison of glass surfaces with functionalized surfaces showed that the functionalized surfaces were superior in terms of sample application reproducibility, mass spectra quality, sensitivity, and information density. Thus, functionalized thin-layer surfaces are considered promising tools for both qualitative and quantitative ADI-MS analysis of complex samples.

Determination of Nicotine in Cigarette Tobacco Smuggled to Brazil by Modified QuEChERS Methodology

The study of cigarette authenticity in Brazil is important due to increasing consumption of contraband cigarettes. Nicotine concentration is an important parameter reflecting the quality of tobacco used in the production of these cigarettes. Simple methods for this determination, which produce reduced waste, are environmentally and industrially important. The nicotine concentration of smuggled cigarette tobacco was determined by the QuEChERS method, requiring some modifications, such as decreasing the volume of the extractor solvent, changes in pH, and removal of the sample hydration step. Quantification was performed by gas chromatography using a flame ionization detector. The Doehlert matrix design was used to optimize the method. The extraction recoveries ranged from 97.5% to 99.6%, with relative standard deviation (RSD) ≤ 2.5% and limits of detection and quantification of 0.6 mg L-1 and 2.5 mg L-1, respectively. The method was sensitive and accurate for the detection and quantification of nicotine. The nicotine concentration in contraband cigarettes was found to be lower than that observed in legal cigarettes. The method was successfully applied to real samples of smuggled and legal cigarettes, providing a robust method for routine analysis and proving the need for more studies on quality control of smuggled cigarettes in Brazil.

A rapid LC-MS/MS method for simultaneous determination of nicotine and its key derivatives including hydroxylation isomers

A rapid high pressure liquid chromatography coupled with tandem mass spectrometry method (LC-MS/MS) was developed for simultaneously determining nicotine (NT) and its derivatives including cotinine (CT), trans-3-hydroxycotinine (HC), nornicotine (NNT), anatabine (AT), anabasine (AB) and amino ketone (4-(methylamino)-1-(3-pyridyl)1-butanone) (AK) as well as two important metabolites isomers 2-hydroxynicotine (2-HN) and 6-hydroxynicotine (6-HN) in tobacco leaves. Different from other methods, 2-HN and 6-HN isomers can be successfully separated and quantified through a combination of amide and C18 columns and optimized multiple reaction monitoring mode of tandem mass spectrometry. The limits of detections (LOD) and quantification (LOQ) of these compounds were 0.02 and 0.06 ng/mL for CT and HC, 0.05 and 0.15 ng/mL for AK and 6-HN, and 0.1 and 0.3 ng/mL for AB, NNT, AT, 2-HN and NT, respectively. Their recoveries ranged from 86.1% for 2-HN to 102.2% for AK by using solvent extraction assisted with ultrasonication. The developed method was validated through determination of the compounds in different regular and genetically modified (for lowering nicotine) tobacco leaves. Conclusively, it is a robust and rapid method for simultaneous quantification of nicotine and its derivatives in tobacco products. The protocol of the method could also be applied in developing analysis method for tracking the toxic substances synthesis and metabolism in tobacco leaves or other herbal plants.

Sensitive Quantification of Nicotine in Bronchoalveolar Lavage Fluid by Acetone Precipitation Combined With Isotope-Dilution Liquid Chromatography-Tandem Mass Spectrometry.

The United States experienced an outbreak of e-cigarette, or vaping, product use-associated lung injury (EVALI) that began in August 2019. Patient diagnosis and treatment sometimes involved bronchoscopy and collection of the bronchoalveolar lavage (BAL) fluid. Although this matrix has been useful for understanding some chemical exposures in the lungs, no methods existed for measuring the nicotine content. Therefore, we developed a simple and sensitive method for measuring nicotine in the BAL fluid. Nicotine was extracted from the BAL fluid using acetone precipitation in a 96-well plate format to increase the sample throughput (200 samples/day). We optimized liquid chromatography column conditions (e.g., mobile phase, column temperature) and mass spectrometry parameters to improve the signal-to-noise ratio and lower limits of detection (LOD) for measuring nicotine in the BAL fluid. The LOD for nicotine in the BAL fluid was 0.050 ng/mL at a sample volume of 40 μL of the BAL fluid. The within-day and between-day imprecision and bias were less than 10%. This method detected nicotine in 15 of 43 BAL fluids from EVALI case patients. This method is useful for understanding recent inhalational exposure to nicotine as part of characterizing EVALI or similar illnesses.

Validation and measurement uncertainty of GC-MS quantification method for nicotine in dried mushrooms using QuEChERS extraction

ABSTRACT Nicotine is an alkaloid and a secondary plant metabolite that has been used as an insecticide. Despite their widespread application, the EU banned the use of nicotine-containing pesticides in December 2008. However, studies in Europe have found nicotine in mushrooms. Nicotine has also been detected in wild mushrooms, so there are other causes of contamination as well as pesticide. This study reports the development of GC-MS method for quantitatively analysing nicotine in mushrooms. This method provides recoveries of 89.5–92.5%, intra-day precisions of 0.32–0.85%, and inter-day precisions of 0.73–2.36%, with limits of detection and quantification of 0.38 and 1.15 μg kg-1, respectively. The relative expanded uncertainty result of 2.8–4.0% complies with CODEX requirements. The method was successfully applied to eleven mushroom samples in which nicotine was detected at levels of 0.033–1.713 mg kg-1. Therefore, this method is suitable for the quantification of nicotine in dried mushrooms to ensure pre-emptive food safety.

Electrochemical determination of nicotine in smokers’ sweat

In this work, we report the demonstration of the capability of electrochemical sensors to provide measurements directly on the sweat of volunteers to successfully discriminate heavy smokers by light ones. Since its importance as pharmaceutical treatment-agent for nicotine replacement therapy for purposes of tobacco cessation, and since that is the key compound for the development of reduced-risk cigarettes by tobacco industry, nicotine is one of those markers that are of interest for monitoring in human sweat. Especially in therapies for purposes of smoking cessation, easy methods for an easy measure of nicotine on the patients is highly required for a fine adjustment of the nicotine dosage as release, e.g., by through therapeutic patches. Therefore, we have checked the use of a simple, inexpensive and very sensitive electrochemical sensor for quantification of nicotine in smokers’ sweat. Proposed sensor is capable for fast, label-free and pretreatment-free detection of nicotine in human sweat as collected directly from the skin of heavy and light smokers. Our finding demonstrates as we can detect nicotine from human skin with sweat-collecting patches thanks to its lower detection limit (0.59 µM) as compared to the physiological limit of nicotine in human blood (12.33 µM), while keeping under control the selectivity with respect various interfering compounds find in sweat too.

Menthol, Nicotine, and Flavoring Content of Capsule Cigarettes in the US.

Characterize physical design features of cigarette brands sold in the US according to the delivery method of menthol that may affect sensory perception among users. Twelve cigarette brands, mentholated and non-mentholated, were purchased for analyses of the physical design characteristics, quantification of nicotine and menthol, and identification of flavor additives. Physical design characteristics did not differ significantly between the various cigarette brands. However, significant differences were seen in levels of menthol. Menthol levels were greatest in products that had dual delivery methods of menthol (6.7mg/cigarette; SE=0.27) followed by products mentholated in a filter capsule only (5.7mg/cigarette; SE=0.25), and those mentholated in the tobacco only (3.8mg/cigarette; SE=0.12); products that were not mentholated had the least (0.38mg/cigarette; SE=0.31). Finally, flavor additives with a mint flavor profile other than menthol were identified, such as pulegone and limonene, and differed between cigarette brands, which are likely contributing to the menthol flavor experience associated with use of these products. The regulation of menthol delivery method, flavorings added to the capsule, and/or menthol concentration may be beneficial for the public health as these factors are likely creating unique sensory experiences.

ANALYSIS OF ILLICIT DRUGS AND NICOTINE IN A BUCCAL TOBACCO BRAND MARKETED IN YEMEN

Objectives: The objective of the present study was to check the potential presence of illicit drugs and to quantify the amount of nicotine in a buccal tobacco brand that had been observed to be increasingly used by Yemeni youths, since 2014, causing narcosis resembling states among them.&#x0D; Methods: Thin-layer chromatography (TLC) described by the United Nations Office on Drugs and Crime (UNODC) was used to screen illicit drugs in the tested brand. The illicit drugs investigated included opiates, heroin, amphetamines, and cocaine. The TLC results were confirmed as recommended by the UNODC using color chemical tests. Identification and quantification of nicotine in the brand was carried out using an appropriate high-performance liquid chromatography (HPLC) system.&#x0D; Results: No illicit drug was found in the tested tobacco brand. On the other hand, it was found that the amount of nicotine in just a single dose (sachet) of the buccal brand was 17.67±0.901 mg, which was 3.53-fold greater than usual buccal dose of nicotine (5 mg).&#x0D; Conclusion: With the exception of cannabis, opioids, and hallucinogens that were not investigated in this study due to technical obstacles, other major illicit narcotic drugs are not found in the brand. The brand contains high amount of nicotine/sachet. However, knowing that the user may use more than one sachet of the brands a day, there is a great potential of nicotine overdosing due to intake of the brand. This may cause a narcosis resembling state called “Nesbitt’s paradox,” characterized by reducing neuronal activity of the user.

Quantification of Nicotine and Cotinine in Plasma, Saliva, and Urine by HPLC Method in Chewing Tobacco Users.

Background:Nicotine acts as major alkaloid of all tobacco products including smokeless tobacco (SLT) forms. The mode of SLT consumption is in the form of chewing under the cheek or lip and induced biochemical alterations in the plasma, saliva, and urine. Materials and Methods:The smokeless tobacco products like Raja or blue bull tobacco brands are widely consumed by human male volunteers under the age of 18-30 years for the period of 3 years consisting of 30g per day. The concentrations of nicotine and cotinine in samples of plasma, saliva, and urine are quantified by the method of HPLC. The remaining variables of plasma are evaluated by auto analyzer and spectrophotometric methods. Results:The analysis of results presented that significant increase in the levels of nicotine and cotinine in plasma, saliva, and urine of chewing tobacco users. The lipid profile (Cholesterol, triglycerides, HDL-C, and LDL-C), liver marker enzymes (SGOT, SGPT, and ALP), kidney markers (Creatinine, urea, and uric acid), glucose, and the remaining variables are present within normal range observed in SLT users. The lipid peroxidation (LPO), nitric oxide (NO) (NO2 and NO3), protein carbonyls (PCO), and peroxynitrites (ONOO-) are reported to be higher levels in the plasma of experimental subjects in comparison with normal controls. The various brands of tobacco varieties (Raja, madhu chhap, hans chhap, miraj, badshah, blue bull, and swagat gold tobacco) are presented. Conclusion:The chewing tobacco users exhibited greater amounts of nicotine and cotinine are at risk of cardiovascular due to nicotine has cardiovascular effects, and oral cancer disease complications in the future for chronic consumption of smokeless tobacco products due to the presence of carcinogens of tobacco-specific N-nitrosamines.

Efficiency Improvement of a Gas Chromatographic-Mass Spectrometric Method for Quantification of Nicotine in Hookah (Water Pipe) Tobacco Products

Efficiency Improvement of a Gas Chromatographic-Mass Spectrometric Method for Quantification of Nicotine in Hookah (Water Pipe) Tobacco Products

A simple dilute-and-shoot method for screening and simultaneous quantification of nicotine and alkaloid impurities in electronic cigarette refills (e-liquids) by UHPLC-DAD

The electronic cigarette (e-cigarette) has emerged as a popular alternative to the traditional hazardous tobacco cigarette. The substantial increase in e-cigarette use also urgently calls for controlling the quality of e-cigarette refill liquid products (e-liquids). Currently, the most important quality indicator of e-liquid products is the quantification of nicotine and its related impurities. Although different methods have been published to measure nicotine and impurity levels, the majority of them use a targeted LC-MS/MS approach. There is, however, a need for more robust quantification methods that are easy to implement in most control (industrial and governmental) laboratories. Therefore, in this study, a simple dilute-and-shoot UHPLC-DAD method has been developed and validated for the simultaneous quantification of nicotine and its alkaloid impurities in electronic cigarette refills. An optimal separation of the alkaloids was achieved in a runtime of 11 min. The method was successfully validated using the “total error” approach in accordance with the validation requirements of ISO-17025. During this validation, interference between the target components and a number of popular flavouring compounds such as vanillin, maltol, ethylacetate, etc. could be excluded. In addition, small changes to the column temperature, pH and molar concentration of the mobile phase buffer were deliberately introduced in order to assess the robustness of the method. Only a slightly different outcome between the newly developed UV-detection method and the targeted MS approach was found, due to the sensitivity of the different detection techniques. However, in the context of quality control of nicotine related impurities, for which the European Pharmacopoeia limits are currently applied, the sensitivity of the UHPLC-DAD method was found to be within the acceptable range. Despite the somewhat lower selectivity of the newly developed UV-detection technique versus a targeted LC-MS/MS approach, it may be concluded that this method is a suitable alternative for quality control purposes.

Quantification of nicotine and major solvents in retail electronic cigarette fluids and vaped aerosols

The main ingredients used in e-liquid formulations (propylene glycol (PG), vegetable glycerol (VG), and nicotine (Nic)) in a total of 16 retail e-liquids (half containing nicotine)) were analyzed by an analytical method developed recently by our research group. The measured nicotine concentrations in e-liquids varied in a range of 5.7 to 14.7 mg g−1 and differed up to 9.0% from the declared label concentrations (two tailed P = 0.5424 at a 0.05 confidence level). The concentrations of Nic and PG in vaped aerosol samples were lower (by an average of 12.7% and 17.4%, respectively) than those in e-liquid samples, while the opposite was true for VG (1.95% higher on average). The composition (w/w) of 16 retail e-liquids varied: 82.6 to 94.9% PG + VG (n = 16), 0.68 to 1.63% nicotine (n = 8), and 5.1 to 16.2% unidentified compounds (water, flavors, etc., n = 16). For the corresponding vaped aerosols, the results were: 75.4 to 92.1% PG + VG (n = 16), 0.57 to 1.47% nicotine (n = 8), and 7.86 to 24.0% unidentified compounds (water, flavors, etc., n = 16). The relative proportion of unidentified compounds in vaped aerosols was two times higher than those in e-liquids. The mean nicotine vaping emission factor (39.6 μg puff−1) was about two times lower than that of a Kentucky 2R4F reference cigarette (75 μg puff−1). The measured e-cigarette vaping emission factors varied in a range of 22.5–61.5 μg Nic puff−1 (n = 8), 1.04–2.45 mg PG puff−1 (n = 16), and 1.35–2.09 mg VG puff−1 (n = 16).

Determination of nicotine content in teeth submitted to prophylaxis and in-office bleaching by gas chromatography-mass spectrometry (GC-MS).

The objective of this study was to evaluate the dental color exposed to acute cigarette smoke treatment and quantify the amount of nicotine in samples exposed to cigarette smoke, after dental prophylaxis and after in-office bleaching. Sixty-nine healthy human molars were subjected to cigarette smoke in a cigarette machine. The teeth were divided into three groups: positive control, prophylaxis, and bleaching. Forty cycles of smoke exposition with duration of 15min each were performed using 10 cigarettes (positive control). Dental prophylaxis was performed with a rotating brush and prophylaxis paste; in-office bleaching was performed with 35% hydrogen peroxide, in two sessions of three 15-min applications, with a 1-week interval between sessions. The color was evaluated at the baseline, after exposure to cigarette smoke, after dental prophylaxis, and after in-office bleaching. Teeth from each group were powdered and analyzed by gas chromatography-mass spectrometry in order to measure the amount of nicotine present in each group. Data from quantification of nicotine and color change were analyzed by one-way ANOVA and Tukey's test (α = 0.05). Data for subjective and objective color evaluation, a perceptible dental darkening occurred in teeth after exposure to cigarette smoke. Dental prophylaxis was able to recover the original color of teeth however, only after bleaching teeth became whiter than at the baseline (p< 0.001). The amount of nicotine was significantly different and higher in positive control group (3.3 ± 1.3μg/g of tooth), followed by the prophylaxis group (2.1 ± 1.4μg/g) and the bleaching group (0.8 ± 0.3μg/g) (p< 0.001). Cigarette smoke penetrates into the dental structure. Dental prophylaxis and bleaching with 35% hydrogen peroxide can partially remove the nicotine from tobacco smoke. However, when in-office bleaching was applied, a more significant nicotine removal was achieved. Dental prophylaxis could remove most of the external nicotine-staining on the tooth surfaces while bleaching could further reduce the external and internal nicotine-staining of teeth.

Paper Spray Mass Spectrometry for High-Throughput Quantification of Nicotine and Cotinine.

The rapid release of new tobacco products requires high-throughput quantitative methods to support tobacco research. Sample preparation for LC-MS and GC-MS is time consuming and limits throughput. Paper spray tandem mass spectrometry (PS-MS/MS) is proposed and validated as a simple and rapid method for quantification of nicotine and cotinine in complex matrices to support tobacco-related research. Air liquid interface (ALI) human tracheobronchial epithelial cell (HTBEC) cultures were exposed to tobacco smoke using a Vitrocell VC-10 smoking machine. Apical culture washes (phosphate buffered saline, PBS) and basolateral media were analyzed with the PS-MS/MS method. GC-MS/MS was used as a comparative quantitative technique. The PS-MS/MS approach allowed for direct spotting of samples on the paper substrate, whereas the GC-MS/MS method required additional sample preparation in the form of solvent-solvent extraction. Limits of quantitation (LOQs) were higher with the PS-MS/MS approach than GC-MS/MS, but still below the relevant concentrations found in HTBEC smoke exposure experiments as well as most clinical applications. PS-MS/MS is readily achieved on mass spectrometers that include atmospheric pressure inlets, and allows for convenient quantification from complex matrices that would otherwise require additional sample preparation and chromatographic separation.

Paper spray mass spectrometry for high-throughput quantification of nicotine and cotinine

Paper spray mass spectrometry for high-throughput quantification of nicotine and cotinine