Quantification Of Methyl Esters Research Articles

Quantification of methyl esters of fatty acids in the oil of Physalis minima by GC-MS

Objective: To investigate quantification of methyl esters of fatty acids in the oil extracted from Physalis minima (P. minima) using gas chromatography-mass spectrometer. Methods: Oil was extracted from the shade dried plant with n-hexane through Soxhlet extraction. Fatty acids that present in the oil were derivatized to fatty acid methyl esters and analysed through gas chromatography-mass spectrometer. Results: A total of nine fatty acids were detected in quantifiable amount in the oil. Both the saturated fatty acids and unsaturated fatty acids were identified. Palmitic acid was found in the highest concentration as 46.83%. Linoleic acid (ω-6) and linolenic acid (ω-3) were obtained in appreciable amount as 16.98% and 14.80% respectively among the unsaturated fatty acids in the oil under study. From the literature review, it appeared that fatty acids were determined for the first time in the oil of P. minima. Conclusions: Presence of these important fatty acids in high amount makes P. minima oil beneficial for health, which can be used in the preparation of phytopharmaceutical or pharmaceutical preparations. Moreover, the results of this study are useful for the phytopharmaceutical industries to establish their quality control profile.

Two-stage processes of homogenous catalysed transesterification of high free fatty acid crude oil of rubber seed

Biodiesel is a diesel replacement and renewable fuel that is manufactured from vegetable oils, animal fats or waste cooking oils. The production of biodiesel from edible oil is currently much more expensive than hydrocarbon-based fuel, due to the relatively high cost of edible oils. The cost of biodiesel can be reduced by using non-edible oils instead of edible oils. The purpose of the present study was to develop a method of esterification of non-edible oil like rubber seed oil (Hevea brasiliensis). The high free fatty acid content oil reacts quickly with alkaline catalysts to form soap, which prevents the separation of biodiesel and glycerol. A two-step process was used instead of the simple alkaline catalysed transesterification process. It consisted of an acid catalysed pre-processing followed by the usual alkaline catalysed process. The physical and chemical properties of biodiesel were analysed. The quantification of methyl esters were done by high-performance liquid chromatography.

Study on Effect of Hydroxyl Group on Lubrication Properties of Palm Based Trimethylolpropane Esters: Development of Synthesis Method

Some concerns have been raised regarding the oxidative stability of vegetable oil-base fluids. Thus, wide ranges of palm-based trimethylolpropane esters, which contain different percentages of partial esters, were synthesized. The palm-based TMP were esterified from Palm Oil Methyl Esters (POME) with trimethylolpropane [2-ethyl-2-(hydroxymethyl)-1,3-propanediol;TMP] and sodium methoxide (CH3ONa) as catalyst. Quantification of methyl esters, mono, di- and tri-TMP esters were performed using a gas chromatography (GC), with a high temperature capillary column (SGE HT5), operated at a temperature gradient of 6°C min-1 starting from 80 to 340°C. The influence of operating variables (temperature, pressure, molar ratio of palm methyl esters to TMP and catalyst amount) on diesters formation was studied and analyzed. Palm oil TMP ester containing 10-30% partial esters (monoesters and diesters) was successfully synthesized.