Quality Of Laboratory Testing Research Articles

Strategic Framework for Strengthening Health Laboratory Services in the COVID-19 Pandemic in Iran.

Providing equitable access to good quality, timely, and affordable laboratory testing has always been a top priority for the Ministry of Health and Medical Education (MoH-ME) and the Reference Health Laboratory (RHL). Considering the significant role of medical laboratories in disease surveillance, RHL developed a strategic plan to manage laboratory services during the COVID-19 pandemic based on the "Strategic Framework for strengthening health laboratory services, 2016-2020" proposed by the World Health Organization (WHO). This article describes the steps taken to establish the strategic framework in Iran. Firstly, a National Laboratory Committee was formed in MoH-ME and a situation analysis was conducted to explore the strengths, weaknesses, opportunities, and threats in different components of our laboratory system. Gaps and resources needed to address those gaps were determined; then, RHL outlined operational processes and mechanisms for monitoring the activities. The WHO strategic roadmap and its six strategic goals concerning leadership, quality, human resources, safety and security, laboratory networking, and rational use of laboratory testing, helped us to promote national laboratory services in accordance with health system requirements in the COVID-19 pandemic. The establishment of a national molecular laboratory network with more than 500 laboratories from different sectors may result in timely access to countrywide laboratory services and would be beneficial for future COVID-19 and/or other viral outbreaks. Continual evaluation of the COVID-19 laboratories' performance, production of PCR test kits by the local manufacturers, and development of a platform for virtual training would be other accomplishments that Iran achieved in coping with the recent pandemic.

Scientific substantiation to updating drinking water quality monitoring programs

Introduction. The results of systematic drinking water quality laboratory tests are the most informative sources. Recommendations on the managing of drinking water quality monitoring were developed in 2020. 
 The purpose of the study was to substantiate methodological approaches to updating drinking water quality monitoring programs.
 Materials and methods. The results of drinking water quality laboratory tests in the Murmansk region, conducted in 2021 in accordance with the monitoring program, were used. The results were analyzed for compliance with the recommendations of the drinking water quality monitoring program. An assessment of the compliance of the results of laboratory studies with hygienic standards and public health risk.
 Results. In Monchegorsk, the drinking water quality was studied at nine control points according to sanitary-chemical, microbiological, and parasitological indicators. The multiplicity of studies per year ranged from 1 to 12 studies. The quality of drinking water in Monchegorsk corresponded to hygienic standards, with the exception of single excesses in the content of chlorine residual free and colour.
 Limitations. When conducting the study, the results of the monitoring were used only for 2021, the results of drinking water quality production control were not taken into account.
 Conclusion. To carry out an objective drinking water quality assessment, it is necessary to update the existing programs of monitoring its quality in the settlements of the Murmansk region carried out within the framework of the social hygienic monitoring (SHM). The authors propose an algorithm for updating monitoring programs

The Development of Germination Tests and Breaking Dormancy Techniques of Bitter Seeds (Andrographis paniculata (Burm. f.) Wall. ex Nees)

Bitter seed (Andrographis paniculata (Burm. f.) Wall. ex Nees) is a medicinal species used in phyto pharmacy and known as ‘the king of bitterness’. Bitter seeds contain andrographolide,
 a diterpenoid lactone which is widely used in Indian medicine for snake bites and for the treatment of hepatitis. The research was conducted at the Seed Management Unit experimental field of the Indonesian Spice and Medicinal Plants Research Institute (IMACRI) and at the Seed Quality Testing Laboratory of IPB University, Indonesia, from January until May 2021. The objectives of the study is to determine the best germination test and effective breaking dormancy techniques to increase the germination percentage of bitter seeds. The study was arranged in a completely randomized design with two-factors. The first factor was seed treatments, i.e., control/untreated, chemical scarification with 5% H2SO4 for 10 minutes, and 5% H2SO4 followed by a 100 ppm GA3 soaking treatment. The second factor conducted was germination test methods according to International Seed Testing Association, i.e., germinating the seeds on the top of paper and in pleated paper. Our study demonstrated that seed treatment with H2SO4 can break seed dormancy and speed up seed germination. Bitter seed germination on the pleated paper was higher than with the top-of-paper method. Bitter seed germination with the top-of-paper method was at 65 DAS and the final germination was at 80 days after sowing (DAS), and the final germination was 55.2%. With the pleated paper method, the seeds germinated at 30 DAS and the final germination was at 70 DAS, and the final the germination of 59.2%.

Preparation and evaluation of certified reference materials for crude protein, crude fat, and crude ash in feed

Three new certified reference materials (CRMs) for crude protein, crude fat, and crude ash in animal feed were developed and characterized according to ISO 17034:2016 and ISO Guide 35:2017. The Kjeldahl, Soxhlet extraction, and ashing methods were employed to quantify these three components in candidate CRMs for swine feed, poultry feed, and fish feed. To improve the precision, the measurement methods were optimized on the basis of Directive 93/28/EEC, Directive 98/64/EC, and Directive 71/250/EEC and further validated using two existing CRMs (BCR-708 and BCR-709). The homogeneity, short- and long-term stability, and moisture content were evaluated. The certified values and their expanded uncertainties (k = 2.00) were determined through nine laboratories. The results for crude protein, crude fat, and crude ash were 14.7 ± 0.4, 5.19 ± 0.24, and 6.23 ± 0.14% for swine feed CRM; 22.7 ± 0.7, 5.16 ± 0.39, and 5.74 ± 0.18% for poultry feed CRM; and 35.6 ± 0.9, 9.29 ± 0.40, and 8.70 ± 0.25% for fish feed CRM, respectively. These three CRMs have been used in the comparison of Chinese feed quality testing laboratory systems and proved suitable for evaluating quality control in laboratories.

Synbiotic Yogurt Fortified with Bawang Dayak (Eleutherine palmifolia) Extract

Aims: The purpose of this research was to study the quality of yogurt symbiotic fortified with Bawang Dayak (Eleutherine palmifolia) extract.
 Study Design: This research used Completely Randomized Design (CRD) experimental method and consist of 4 treatments.
 Place and Duration of Study: Sample: Animal Product Technology Laboratory Faculty of Animal Science Universitas Brawijaya, Food Quality and Safety Testing Laboratory Department of Agricultural Product Technology, Faculty of Agricultural Technology Universitas Brawijaya, between June 2022 – December 2022.
 Methodology: The material used are skim milk, fresh milk, yogurt starter bacteria (Lactobacillus bulgaricus, Streptococcus thermophilus, Lactobacillus acidophillus), and bawang dayak extract. Bawang Dayak were extracted using Microwave-Asisted Extraction (MAE) with temperature 60-700C. This research which consist of 4 treatments and 3 replications with various concentration of Bawang Dayak extract of T0 (0%), T1 (1%), T2 (4%), and T3 (6%), respectively. The samples of synbiotic yogurt with fortification of Bawang Dayak extract were analyzed for their total lactic acid bacteria, antioxidant activity, viscosity and color analysis.
 Results: Fortification of Bawang dayak extract in synbiotic yogurt results in highly significant different effects on the quality parameters of antioxidant and color (lightness, redness and yellowness) (P<0.01), but had no significant effect (P>0.05) on the total quality of lactic acid bacteria. Fortification of 6% Bawang dayak extract (T3) in yogurt produced the best quality in terms of antioxidant content and color (lightness, redness, and yellowness).
 Conclusion: The best treatment of synbiotic yogurt was achieved in T3 with the 6% of Bawang Dayak extract.

Modelling the Structure and Anisotropy of London Clay Using the SA_BRICK Model

Several constitutive models had been developed by other researchers to cover the main features of mechanical behaviour of natural overconsolidated clays, such as the nonlinear stress–strain response at small and large strains, and the recent stress history effect. Kinematic hardening models include these features to facilitate realistic predictions of soil–structure interaction. This paper presents the further development of a kinematic hardening model BRICK that includes anisotropy and the influence of soil structure on the mechanical behaviour of a natural clay. High quality laboratory tests were used to calibrate the input parameters of the model in a single element configuration, and a documented boundary value problem of tunnel excavation was used to validate the model in finite element calculations. A comprehensive comparative study between the predictions of different kinematic hardening models, using two different software packages, was carried out. It was observed that the SA_BRICK model is in fair agreement with the observed data and gives improved predictions in comparison to other kinematic hardening models, particularly in terms of narrowness of the settlement trough above the tunnel. Advanced predictions of ground deformations caused by tunnel excavations can be effectively used to mitigate possible damage of existing structures affected by tunnelling in an urban environment.

水道水中農薬のGC/MSスクリーニング分析におけるクロマトグラム解析の誤差要因の分析

In order to adopt "screening analysis" in drinking water quality testing, which performs qualitative and quantitative analysis based on information registered in a database without measuring standard materials at the time of each testing, a validation test was conducted to evaluate the difference on the chromatogram data analysis by analysts. In the validation test, chromatograms of 6 water samples were analyzed using the same database and software by 16 analysts in 13 laboratories. Peak identification was performed based on Retention time (RT), (qualifier and target ion) QT ratio, and degree of matching of mass spectra, and if necessary, peak shapes in water samples were compared with those in calibration curve samples. Large differences were observed in the detection of pesticides among the analysts. There were two main reasons for the disagreement. The first is that the judgement of peak detection at low concentrations near the quantification limit of each pesticide, particularly below the concentration at three times of quantification limit, differed depending on the analyst. The other reason was that some analysts made clear misidentifications and identified wrong peaks close to the predicted retention time, despite the QT ratio and mass spectrum didn't match that in the database. In addition, there were some cases where the quantified values were reported by misidentifying the peaks of degradation products and metabolites, even when the peak detection was correctly judged. In order to use screening analysis practically by drinking water quality testing laboratories, it is important to conduct a validation test like this study in advance to use screening analysis and to coordinate criteria for peak identification and quantification among data analysts.

Model Pendugaan Energi Metabolis Pakan dan Bahan Pakan Ayam Broiler Berdasarkan Analisis Proksimat dan Energi Bruto

This study aimed to make estimation model of metabolizable energy (ME) of broiler chicken ration and feedstuffs based on proximate analysis and gross energy (GE). The estimation model used multiple and simple linear regression analysis with in vivo data approach. A total of 34 starter commercial ration data, 33 finisher commercial ration data, 17 experimental ration data, 14 corn data, 8 palm kernel cake (PKC) data from Center for Quality Testing and Feed Certification Laboratory and other research while the moisture content (MC), ash, crude protein (CP), ether extract (EE), crude fiber (CF), GE and ME were used as database and analyzed using SPSS version 20. The results showed that ME estimation using multiple regression equation for starter commercial ration ME = 2,444.89 + 201.34x(MC) - 415.85x(ash) - 0.87x(CP) + 282.58x(EE) 284.96x(CF), finisher commercial ration ME = 6,451.05 - 244.99x(MC) - 226.72x(ash) - 101.40x(EE) + 363.12x(CF), experimental ration ME = 3,001.07 + 41.39x(MC) - 19.49x(ash) - 12.82 x(CP) + 87.31x(EE) - 160.99x(CF), corn ME= 8,504.07- 301.32x(MC) + 196.41x(ash) -252.58x(CP) + 114.52x(EE) + 47.99x(CF) and PKC ME = 6,197.28 - 111.45x(CF) while linear equation for starter commercial ration ME= 3,821.79 - 0.15GE, finisher commercial ration ME = 12,266.23 - 2.19 (GE), research ration ME= 1,416.83 + 0.398x(GE), corn ME= -2,958.80 + 1.68x(GE), PKC ME = -5,769.41 + 2.09x(GE). These two models could be used to estimate ME content in commercial finisher ration, experimental ration, corn, and PKC whereas both models could not be used for commercial feed starter.
 Key words: chicken feed, corn, metabolizable energy, palm kernel cake, regression analysis

Comparison Of Local And Import Sands Quality Laboratory Tests Results For Used In Hydraulic Fracturing Operations

Sand quality laboratory tests have been carried out on local and import uncoated sands. Based on results of sand sieve, roundness, sphericity, turbidity, acid solubility and crush resistance at 3000 psi quality tests of import uncoated sand has better quality than local sand. The import sand fulfills API – RP 56 specification requirements and will be able used in hydraulic fracturing operation. Whereas, the local uncoated sand does not achieve API - RP 56 specification requirements.

The Quality of Yellow Fin Tuna Powder (Thunnus Albacores) as a Natural Flavoring Alternative

Flavoring is already commonly used by the community, but on the market, flavoring generally contains monosodium glutamate (MSG). Alternative processed yellow fin tuna (Thunnus albacores) into natural flavoring fish powder without MSG becomes interesting and very useful for reducing MSG intake in the people of North Maluku. This type of research is experimental research conducted in September - November 2018 in the SIG Laboratory, Bogor for chemical quality testing (sodium, water, protein, and total fat) and Food Organizing Laboratory for Nutrition Department of Health Ministry of Ternate for making fish powder and organoleptic quality tests (color, texture, aroma, and taste). Chemical test results obtained sodium content of 3204.79 mg / 100 g of sample, moisture content of 6.08%, protein of 48.76%, and total fat of 3.28%; and the organoleptic test results obtained a mean ± SD of the color aspect of 4.0 ± 0.7, texture of 3.5 ± 0.9, aroma of 4.1 ± 0.8, and taste of 3.7 ± 1, 0. It was concluded that the heating process (roasted and oven-dried) affected the chemical quality of yellow fin tuna powder, and obtained sodium 3204.79 mg / 100 g, Water content 6.08%, protein content 48.76%, and total fat 3.28%; and in terms of organoleptics, the average panelist likes color and aroma, while aspects of taste and texture are only part of the panelists who say they like.

Maize seed quality with fungicide treatment during the storage period

In some areas, farmers sometimes delay planting due to several factors, including: unfavorable climate, unprepared land and insufficient manpower so that planting takes a long time. As a result, seeds that have been given a fungicide cannot be planted and stored under uncontrolled conditions, both at the place and temperature of the storage room. Therefore, the purpose of this study was to determine the storage resistance of seeds that have been given a fungicide (saromyl) so that they can be used as a reference for storing seeds of saromyl. The research was carried out in June - December 2020 at the seed quality testing laboratory of the Cereal Crops Research Institute (Balitsereal), Maros, South Sulawesi Province. The corn seeds used in the study were Bisma 2018 and 2019, Sukmaraga 2017 and 2019, Srikandi Kuning 2018 and 2019, Lamuru 2017 and 2019 varieties. Observations were made on 1000 grain weight, moisture content, electrical conductivity, maximum growth potential, seed growth speed, length of primary root, length of shoot and number of secondary roots. This study used a completely randomized design with 4 replications. The results showed that at room temperature storage (25-26°C and relative humidity 50-58%) the seeds that had been given a fungicide (saromil) with a storage period of 21 days still had high seed quality. Even at low temperature storage (18-18.9°C and Rh 50-55%) during the 6-month storage period, the quality of the seeds was very good. The shelf life of seeds that have been given a fungicide (saromil), is influenced by the shelf life of the seeds, the temperature and relative humidity of the storage room.

Liquid chromatography-tandem mass spectrometric method for trace quantification of ethyl methanesulfonate: a genotoxic impurity in dapoxetine hydrochloride

BackgroundDapoxetine hydrochloride is a selective serotonin reuptake inhibitor drug for treating premature ejaculation. This study was designed to develop and validate a sensitive and selective LC–MS/MS method for trace analysis of genotoxic impurity ethyl methanesulfonate in Dapoxetine hydrochloride.ResultsChromatographic separation was achieved on the Shodex RSpak DS-413 column, 150 × 4.6 mm, 3.0 µm using eluent containing a equal volumes of acetonitrile and 0.1% v/v formic acid in water was used in the isocratic elution mode at a pump flow of 1.0 mL/min. No interference was observed at the retention time of ethyl methanesulfonate, indicating that the developed method is specific and selective for trace level quantification.The developed method was found to be linear in the concentration range of 1–50 ppm with coefficient of regression of 0.9997. Detection limit and quantification limit were determined to be 0.6 ppm and 1.0 ppm respectively. Acceptable RSD values (< 10.0%) and recovery results (> 90%) obtained from the accuracy and precison experiments indicate that the developed method is precise and accurate in the concentration range of 1–50 ppm. Ethyl methanesulfonate solutions were stable for two days when stored at room and refrigerated temperatures.ConclusionThe developed method has the ability to quantify ethyl methanesulfonate in dapoxetine hydrochloride. Thus, the anticipated method has high probability to adopt in the quality testing laboratories of pharmaceutical industry.

Kualitas Fisik Biskuit Pakan yang Berbeda Bahan Penyusunya

This study aims to determine the physical quality (water content, water absorption, and density) of corn leaf biscuits and corn husk biscuits compared with field grass biscuits conducted in the Animal Feed laboratory and the Feed Quality Testing Laboratory of the Polytechnic of Agriculture Development Yogyakarta-Magelang. The study was conducted using a Completely Randomized Design (CRD) with 3 treatments and 6 replications, namely biscuits from field grass and 15% molasses as a control (P0), corn leaf biscuits and 15% molasses (P1) and biscuits from corn husk and molases 15% (P2). The results of P0, P1 and P2 were equally good, in the physical quality test of the absorption capacity and density of feed biscuits. The lowest water content (P<0.05) was found in corn husk biscuits (P2). It was concluded that the biscuits, corn leaves and corn husk produced the same physical quality of water absorption and feed biscuits density, except for the water content, namely corn husk biscuits (P2) had the lowest water content values ​​compared to corn leaf biscuits and field grass biscuits.

Effect of Differences in Salt Concentration on the Quality of Rebon Shrimp Paste (Acetes Sp) in Tegal District

Rebon Shrimp Paste (RSP) in Indonesia uses different percentages of salt addition, ranging from 2 to 20% or not at all. This study aims to determine the influence of different salt concentration (5%, 10%, 15% and without salt) on the quality of RSP organoleptic, microbiological and chemical. This research was conducted in Munjung Agung, Tegal and Cirebon Fisheries Product Quality Testing and Application Laboratory. The results showed that the addition of different salt concentration (5%, 10%,15% and without salt) affected the quality of organoleptics, microbiology, and chemistry. Organoleptic quality with salt concentration of 5% and 10% favored panelists with an average value of 6.8 (not yet meeting Indonesian National Standards). The highest water content value is found in RSP that are not added salt (40,19%-43,22%) and lowest at 15% salt concentration (31,12%-34,82%) in accordance with the SNI. E.Coli and Salmonella growth was negative in all four samples and didn’t different materially, according to SNI standard 2716.1:2009. The best increase in salt concentration in the study was 5%, with an average organoleptic value of 6.8 in the coliform contamination sample A2 APM/g < 3, negative E.Coli and Salmonela with a water content value of 39.15.

Consolidation parameters in silts from varied rate CPTU tests

&lt;abstract&gt; &lt;p&gt;The interpretation of dissipation tests from cone penetration tests (CPTU) in silt is often considered challenging due to the occurrence of an unknown degree of partial consolidation during penetration which may influence the results significantly. The main objective of the present study is to investigate the influence of penetration rate and hence partial consolidation in silt deposits on the interpretation of consolidation parameters. Rate dependency studies have been carried out so as to give recommendations on how to establish design consolidation parameters in silts and consider the effect of partial consolidation on the development of design parameters. A comprehensive field and laboratory research program has been conducted on a silt deposit in Halsen-Stj?rdal, Norway. Alongside performing various rate penetration CPTU tests with rates varying between 0.5 mm/s and 200 mm/s, dissipation tests were executed to analyze the consolidation behaviour of the soil deposit. Furthermore, a series of soil samples have been taken at the site to carry out high quality laboratory tests. Correction methods developed for non-standard dissipation tests could be successfully applied to the silt deposit indicating partial consolidation. The results revealed an underestimation of the coefficient of consolidation if partial consolidation is neglected in the analysis, emphasizing the importance of considering the drainage conditions at a silt site thoroughly. To study the drainage conditions of a soil deposit a recently proposed approach has been applied introducing a normalized penetration rate to differentiate between drained and undrained behaviour during penetration. It is suggested that a normalized penetration rate of less than 0.1–0.2 indicate drained behaviour while a normalized penetration rate above 40–50 indicate undrained behaviour. Finally, available dissipation test data from a Norwegian Geo-Test Site (NGTS) in Halden, Norway have been used to successfully verify the recommendations made for silts.&lt;/p&gt; &lt;/abstract&gt;

APPLICATION OF MACHINE LEARNING MODELS IN ENROLLMENT AND STUDENT TRAINING AT VIETNAMESE UNIVERSITIES

In Vietnam, since 2015, the Ministry of Education and Training of Vietnam has decided to abolish university entrance exams and advocates the use of high school graduation exam results of candidates for admission to go to universities. The 2015 and 2016 exam questions for the Math exam are the essay questions. From 2017 up to now, the Ministry of Education and Training of Viet nam has applied the form of multiple-choice exams for Mathematics in the high school graduation exam. There are many mixed opinions about the impact of this form of examination and admission on the quality of university students. In particular, the switch from the form of essay examination to multiple-choice exams led the entire Vietnam Mathematical Association at that time to send recommendations on continuing to maintain the form of essay examination for mathematics. The purposes of this article are analysisand evaluation the effects of relevant factors on the academic performance of advanced math students of university students, and offer solutions to optimize university entrance exam. The data set was provided by Training Management Department and Training Quality Control and Testing Laboratory of the University of Finance – Marketing. This dataset includes information about math high school graduation test scores, learning process scores (scores assessed by direct instructors), and advanced math course end test scores of 2834 students in courses from 2015 to 2019. Linear and non-linear regression machine learning models were used to solve the tasks given in this article. An analysis of the data was conducted to reveal the advantages and disadvantages of the change in university enrollment of the Vietnamese Ministry of Education and Training. Tools from the Python libraries have been supported and used effectively in the process of solving problems. Through building and surveying the model, there are suggestions and solutions to problems in enrollment and input quality assurance. Specifically, in the preparation of entrance exams, the entrance exam questions should not exceed 61-66 % of multiple choice questions.

Methodological Approaches to Organization of Drinking Water Quality Monitoring Programs

Summary. Introduction: Drinking water is one of the most important environmental factors sustaining life and determining human health. The goal of the Russian Federal Clean Water Project is to improve drinking water quality through upgrading of water treatment and supply systems using advanced technologies, including those developed by the military-industrial complex. The most informative and reliable sources of information for assessing drinking water quality are the results of systematic laboratory testing obtained within the framework of socio-hygienic monitoring (SGM) and production control carried out by water supply organizations. The objective of our study was to formulate approaches to organizing quality monitoring programs for centralized cold water supply systems. Materials and methods: We reviewed programs and results of drinking water quality laboratory tests performed by Rospotrebnadzor bodies and institutions within the framework of SGM in 2017–2018. Results: We established that drinking water quality monitoring in the constituent entities of the Russian Federation differs significantly in the number of monitoring points (566 in the Krasnoyarsk Krai vs 10 in Sevastopol) and measured indicators, especially sanitary and chemical ones (53 inorganic and organic substances in the Kemerovo Region vs one indicator in the Amur Region). Discussion: For a more complete and objective assessment of drinking water quality in centralized cold water supply systems, monitoring points should be organized at all stages of water supply with account for the coverage of the maximum number of people supplied with water from a particular network. Thus, the number of points in the distribution network should depend, inter alia, on the size of population served. In urban settlements with up to 10,000 inhabitants, for example, at least 4 points should be organized while in the cities with more than 3,000,000 inhabitants at least 80 points are necessary. We developed minimum mandatory lists of indicators and approaches to selecting priority indices to be monitored at all stages of drinking water supply.

A MASS COMPATIBLE UPLC METHOD FOR THE QUANTIFICATION OF IMPURITIES IN FLUTICASONE PROPIONATE NASAL SPRAY

Objective: The objectives of the present study were to develop and validate a mass compatible ultra-performance liquid chromatography (UPLC) method to quantify the impurities in fluticasone nasal spray, and to establish a suitable container-closure system for the formulation.&#x0D; Methods: A gradient method was optimized with a flow rate of 0.5 ml/min, detector wavelength-240 nm, run time-25 min and 0.1% Trifluoroacetic acid (TFA) in water as solvent A and Methanol as solvent B.&#x0D; Results: The developed method was linear over the range of 0.07-1.10 µg/ml for impurity-I, 0.16-2.47 µg/ml for impurity-II, 0.67-10.0 µg/ml for impurity-III, and 1.29-19.3 µg/ml for impurity-IV. The limit of quantification (LOQ) and limit of detection (LOD) were established as 0.07 and 0.02 µg/ml, 0.14 and 0.05 µg/ml, 0.59 and 0.19 µg/ml, 1.06 and 0.35 µg/ml for impurities I-IV respectively. The percent relative standard deviation (%RSD) of the replicate analysis for impurities I-IV, was within the acceptance criteria (0.4, 0.2, 0.3, and 0.1% respectively) that proved the precision of the method. The accuracy of the method was studied from 50%-150% of test concentration and the results ranged from 100.3% to 109.4%. The container-closure compatibility study revealed that the solution stored in the glass container system did not generate any additional peaks in the chromatogram.&#x0D; Conclusion: Hence, the developed method can be employed by quality testing laboratories to quantify impurities in fluticasone propionate nasal spray. The study also suggests that glass containers could serve as a compatible system for the storage of fluticasone propionate nasal solution.

Analysis Of Protein Levels With Giving Kelor Leaf Extract (Moringa Oleifera) On Soy Milk

Soy milk is a beverage product that is currently being favored by the people of Indonesia, to increase the protein content in soy milk needs to be substituted for other ingredients using Moringa Oleifera leaf extract. The purpose of this study was to analyze the organoleptic acceptability of soy milk by giving Moringa Oleifera leaf extract.&#x0D; This research is quantitative research with experimental research design using the True Experiment research method. This research was conducted in December 2018 until February 2019 at the Food Quality and Safety Testing Laboratory of the Agricultural Product Technology Department of the Faculty of Agriculture, University of Brawijaya Malang to test the protein content while the Organoleptic Test was carried out at STIKeS Satria Bhakti Nganjuk. The sample size is 20 panelists. The independent variable in this study was Moringa leaf extract while the protein content of soy milk. Data was collected using an organoleptic sensory test assessment table and a protein content test sheet using the Kjeldahl method. Data were analyzed using ANNOVA test with the results of H0 rejected and H1 accepted with a significant value &lt;0.05&#x0D; The results of ANOVA analysis get F count value of 105,405 and F table for testing 20 samples to get F table 4.35. So that it can be concluded that the addition of Moringa leaf extract 0% to 10% Significant and simultaneous organoleptic.&#x0D; Addition of Moringa Oleifera leaf extract to soy milk affected organoleptic acceptability and increased protein content of soy milk in accordance with the percentage of Moringa leaf extract levels added.

Effect of Physiological Maturity and Germinating Media on Zanzibar Clove Seeds Viability

&lt;em&gt;Low seeds quality of the clove tree may attribute to the low national productivity of cloves in Indonesia. The study aimed to determine the level of physiological fruit maturity and the effect of germination media on the viability of clove seeds. The study was performed at the Leuwikopo Seeds Storage and Quality Testing Laboratory and Leuwikopo Research Installation, Bogor, in October 2017 to December 2018. The material used was the Zanzibar clove seeds from ≥ 10 years old trees. The clove seeds obtained from a farmer’s plantation in Tolitoli District, Central Sulawesi. The study arranged in a split-plot design, with four replications, with the main plot was four levels of seed viability based on fruit colors: reddish-green, pink, red, and dark red. The subplots were three types of germination media, namely sand, cocopeat, and zeolite. Thus, there were twelve treatments. Each plot consisted of 40 seeds for germination test and five seeds for water content determination. The results showed that the percentage of clove seeds viability negatively influenced by the level of fruit maturity based on the color of the fruit but was affected by the germination media. Good physiological quality of clove seeds characterized by dry seed weight of 2.70 g, 100 % germination rate, vigor index of &lt;em&gt;73.75 %, and growth rate of 1.19 % etmal&lt;sup&gt;-1&lt;/sup&gt;. Among the three types of germination media tested (sand, cocopeat, and zeolite), sand was the best germination media for clove seeds. Hence it is recommended to seeds producer.&lt;/em&gt;&lt;/em&gt;