Shoot Quality Research Articles

Expansion of Pleioblastus amarus in tea plantations significantly enhances the appearance and nutritional composition of bamboo shoots but adversely affects palatability

The expansion of Pleioblastus amarus into tea plantations introduces environmental heterogeneity, significantly influencing the growth and quality of bamboo shoots. This study examined the effects of bamboo expansion on the appearance, nutrition, and palatability of bamboo shoots, utilizing partial least squares structural equation modeling (PLS-SEM) to identify key influencing factors. Results revealed that bamboo expansion increased shoot diameter, length, and fresh weight, enhancing overall size and edibility, particularly in the tea-bamboo mixed forest center zone (TBC), where appearance quality peaked. Nutritional analysis revealed substantial increases in protein, fat, starch, and vitamin C content after bamboo expansion, along with the improvements in amino acid score (AAS), essential amino acid index (EAAI), and nutritional index (NI), indicating elevated nutritional value. However, despite the rise in soluble sugars and flavor-enhancing amino acids, higher levels of total acids, oxalic acid, tannins, and cellulose diminished the palatability, notably in TBC site. PLS-SEM further indicated that while bamboo expansion positively influenced shoot appearance and nutrition, soil factors predominantly drove these changes and concurrently detracted from overall palatability. These findings provide a framework for enhancing bamboo shoot quality and optimizing management practices in tea plantation ecosystems.

Response of GA3, NAA, and KNO3 treatments on in vitro seedgermination and shoot performance of highland bamboo(Oldeania alpina L.)

Oldeania alpina (K. Schum) Stapleton is an African alpine bamboo, native of Ethiopia to Zambia. The untreated seeds of O. alpina do not give good germination. Therefore, the effects of different seed pretreatments on germination and shoot quality were evaluated. The pre-sowing treatments were highly significant (P ≤ 0.001) for all studied parameters. The seeds subjected to 8% Gibberellic acid (GA3) treatment produced the highest germination percent (30%) while 6% NAA naphthalene acetic acid produced 23% germination. The values of MGT (mean germination time), PV (peak value) and VI (vigorous index) were also analyzed and interpreted to evaluate the germination of seed and shoot quality of the emerging plants. Moreover, the highest values for morphological parameters were recorded for 8% GA3 and 6 % NAA. Hence, the use of 8% GA3 or 6% NAA pretreatment is recommended to enhance the germination and shoot quality of O. alpina.

Multifaceted analyses reveal sugar and organic acid metabolism affecting the palatability in mulched Phyllostachys violascens shoots with varied sheath colors

The color of bamboo shoot sheaths influences both appearance and palatability in mulched Phyllostachys violascens shoots, but the mechanisms linking sheath color to flavor and texture are unclear. This study combined metabolomics and transcriptomics to investigate the relationship between sheath color and palatability, focusing on sugar and organic acid metabolism. We identified 5698 metabolites and 33 differentially expressed genes across bamboo shoots with different sheath colors. Darker shoots (black-brown, BSD) exhibited enhanced organic acids and flavor amino acid synthesis, contributing to moderate sweetness. In contrast, as the sheath color lightens (bright-red, BSM; yellow-green, BSL), sugar degradation and cell wall metabolism (e.g., lignin, pectin, cellulose) became more pronounced. BSL shoots, with the lightest sheath color, demonstrated reduced sugar degradation and increased synthesis of pectin and cellulose, resulting in greater sweetness and improved texture. These findings demonstrate that variations in sheath color are closely linked to metabolic shifts that influence both flavor and texture, offering valuable insights for targeted breeding programs aimed at enhancing bamboo shoot quality.

Efficient in-vitro regeneration protocol for large-scale propagation of Aloe vera (L.) Burm.f.

Aloe vera a medicinal shrub, is used in pharmaceuticals and cosmetics due to its therapeutic properties, but its male sterility and self-incompatibility make seed-based reproduction challenging. The current investigation provides a detailed account of a refined in-vitro regeneration process, discussing direct and indirect organogenesis techniques. Shoot multiplication, callus formation, organogenesis in callus culture, and rooting were all evaluated in the Murashige and Skoog (MS) medium employing several plant growth regulators (PGRs). BAP (N6-benzyl amino purine) alone at 3.5 mg/l was the most effective treatment for rapid shoot multiplication and the healthiest shoot quality in direct shoot proliferation. It was shown that a combination of 2.0 mg/l Kn (Kinetin) and 1.0 mg/l NAA (1-naphthalene acetic acid) was most responsive for regeneration in callus culture, whereas 2.5 mg/l 2,4-D (2,4-dichloro phenoxy acetic acid) produced the most profuse regenerative callusing. In addition, plantlets treated with 1.5 mg/l IBA (indole-3-butyric acid) in-vitro produced the highest and longest roots, contributing to a 94 % survival rate during the subsequent acclimatization process. Moreover, regeneration efficiency via direct and indirect channels is also briefly discussed. This research has the potential to enhance the efficiency, precise application of appropriate PGRs needed for the mass production of Aloe vera at various stages of in-vitro culture.

Effect of Ancymidol and Phloroglucinol on the Number and the Quality of Shoots in the Micropropagation of Date Palm (Phoenix dactylifera L.)

Effect of Ancymidol and Phloroglucinol on the Number and the Quality of Shoots in the Micropropagation of Date Palm (<i>Phoenix dactylifera</i> L.)

Integrated Transcriptome and Metabolome Analyses Provided Insight into the Variation in Quality of Green Bamboo (Bambusa oldhamii) Shoots at Different Growth Stages

Green bamboo (Bambusa oldhamii) shoots are not only delicious but also highly nutritious. However, their palatability and quality changed significantly upon being unearthed, making them unsuitable for commercial sale and subsequently diminishing their market value. To clarify the mechanisms that regulate the quality of green bamboo shoots at different growth stages, we conducted a comprehensive analysis of the metabolome and transcriptome at the unearthed height of 0 cm (H0), 5 cm (H5), and 10 cm (H10). Metabolome analysis identified 149 differentially accumulated metabolites (DAMs) among H0, H5, and H10, primarily comprising phenolic acids, lipids and flavonoids. Metabolic pathways enriched by these DAMs included phenylpropanoid biosynthesis, phenylalanine metabolism, flavonoid biosynthesis, linoleic acid metabolism and alpha-linolenic acid metabolism. Further transcriptome analysis identified 2976 differentially expressed genes (DEGs) among H0, H5, and H10. Additionally, KEGG analysis indicated that these DEGs mainly enriched pathways associated with metabolic pathways, biosynthesis of secondary metabolites, and phenylalanine metabolism. We screened out 10 DEGs and 16 intermediate metabolites of these pathways. Furthermore, we identified six DEGs with expression patterns highly correlated with the content of lignin and the total flavonoids from H0 to H5 and H10. Finally, RT-qPCR analysis of six genes validated the transcriptome sequencing data. Our findings indicate significant quality variations in green bamboo shoots from H0 to H5 and H10. These variations are closely related to key genes involved in the synthesis of lignin and flavonoids, which result in the flavor and quality changes of green bamboo shoots from the belowground stage to unearthed stage.

Foliar spraying KH2PO4 promotes shoot development by inducing stomatal opening and sugar transport in tea leaves

Tea plant is a perennial woody plant that is mostly harvested for its new shoots. Photoassimilates made from mature leaves are the material basis for the formation of yield and quality of new shoots. Potassium dihydrogen phosphate (KH2PO4) as a common foliar fertilizer has been widely used to improve crop yield and quality formation. However, the effect of KH2PO4 on photosynthesis, photoassimilates formation and transportation in tea plants and underlying molecular mechanism remain unclear. Therefore, in the present study, physiological, metabolomics, and transcriptomics analysis between tea plants spraying KH2PO4 and control were performed. The results showed that spraying KH2PO4 could significantly enhance the growth of new shoots. Further, spraying KH2PO4 could effectively increase the proportion of stomatal opening of mature leaves, and significantly increase net photosynthetic rate (Pn), transpiration rate (Tr), and stomatal conductance (Gs). The targeted metabolomics analysis of phloem exudates revealed a significant elevation of sorbitol, arabinitol, d-glucuronic acid, and d-arabinose in mature leaves spraying KH2PO4. Combined with the transcriptome sequencing results, it was found that spraying KH2PO4 significantly induced the up-regulation of genes controlling stomatal opening including HAK17, POT2, ALMT2, ALMT14, PYL4 and PYL2. And the expression levels of photosynthetic antenna proteins such as RA, LHCII type III CAB-13, Lhca3 and LHCI type III LHCA6 were also significantly up-regulated. At last, we found that two polyol transporters, PLT4 and PLT5 were up-regulated. The results revealed the molecular mechanism of KH2PO4 in regulating stomatal opening and the transportation of photoassimilates, laying a theoretical foundation for the application of KH2PO4 in tea plants.

Identification of the Key Gene DfCCoAOMT1 through Comparative Analysis of Lignification in Dendrocalamus farinosus XK4 and ZPX Bamboo Shoots during Cold Storage.

Dendrocalamus farinosus bamboo shoots, a species with rich nutritional value, are important in Southwest China. Lignin is an important factor affecting the postharvest flavor quality of bamboo shoots; however, the underlying mechanism of lignin deposition in D. farinosus bamboo shoots during cold storage is still not fully understood. In this study, the mutant D. farinosus XK4 with low lignin content at 3.11% and the cultivated variety ZPX at 4.47% were used as experimental materials. The lignin content of D. farinosus XK4 and ZPX, as well as the gene expression differences between them, were compared and analyzed during cold storage using transcriptomic and physiological methods. Our analysis revealed several key genes and found that D. farinosus CCoAOMT1 plays a key role in the regulatory network of bamboo shoots during cold storage. Tobacco heterologous transformation experiments demonstrated that overexpression of DfCCoAOMT1 significantly increases lignin content. This study provides a novel foundation for future research aimed at improving the postharvest quality and flavor of D. farinosus bamboo shoots through targeted genetic manipulation during cold storage.

Effects of melatonin on inhibiting quality deterioration of postharvest water bamboo shoots

Water bamboo shoots (Zizania latifolia) is prone to quality deterioration during cold storage after harvest, which causes the decline of commodity value. Chlorophyll synthesis and lignin deposition are the major reasons for quality degradation. This paper studied the influence of exogenous melatonin (MT) on the cold storage quality of water bamboo shoots. MT treatment could delay the increase in skin browning, hardness and weight loss rate, inhibit chlorophyll synthesis and color change of water bamboo shoots, while maintain the content of total phenols and flavonoids, and inhibit lignin deposition by inhibiting the activity and gene expression of phenylpropanoid metabolism related enzymes as PAL, C4H, 4CL, CAD, and POD. The results indicate that exogenous MT treatment can effectively inhibit the quality degradation of cold stored water bamboo shoots.

Growing Salicornia europaea L. with Saline Hydroponic or Aquaculture Wastewater

Among halophyte plants, Salicornia species (also known as glasswort or sea asparagus) are increasingly grown in open fields and greenhouses for edible or non-edible purposes. Their salinity tolerance makes it possible to irrigate Salicornia plants with saline waters and even seawater, which cannot be used by other crop species. In this work, S. europaea (L.) was cultivated in pots under the typical climatic conditions of the fall season in the Mediterranean region and irrigated with non-saline standard nutrient solution (SNS) or saline wastewater discharged from a greenhouse semi-closed hydroponic (substrate) culture of tomato or a saltwater recirculating aquaculture system (RAS) with Gilthead sea bream (Spaurus aurata L., which was used as such or after dilution (50:50) with SNS. Plant growth was not significantly affected by the composition of irrigation water, while higher antioxidant capacity (measured using the DPPH assay) and concentration of photosynthetic pigments, phenols, flavonoids, and ascorbic acid were found in the shoots of SNS plants than in those of plants irrigated with wastewater. The level of lipid peroxidation and H2O2 production significantly increased in the SNS plants, which also showed higher activity of superoxide dismutase and lower activity of catalase. These results suggest that S. europaea can be cultivated using wastewater with moderate to high salinity discharged from greenhouse hydroponic crops or RASs, and that salt is not strictly required for the growth of this species. Using non-saline nutrient solution can result in moderate oxidative stress that improves the shoot quality of S. europaea.

Chromosomal-level genome and metabolome analyses of highly heterozygous allohexaploid Dendrocalamus brandisii elucidate shoot quality and developmental characteristics.

Dendrocalamus brandisii (Munro) Kurz is a sympodial bamboo species with inimitable taste and flavorful shoots. Its rapid growth and use as high-quality material make this bamboo species highly valued for both food processing and wood applications. However, genome information for D. brandisii is lacking, primarily due to its polyploidy and large genome size. Here, we assembled a high-quality genome for hexaploid D. brandisii, which comprises 70 chromosomes with a total size of 2,756 Mb, using long-read HiFi sequencing. Furthermore, we accurately separated the genome into its three constituent subgenomes. We used Oxford Nanopore Technologies long reads to construct a transcriptomic dataset covering 15 tissues for gene annotation to complement our genome assembly, revealing differential gene expression and post-transcriptional regulation. By integrating metabolome analysis, we unveiled that well-balanced lignin formation, as well as abundant flavonoid and fructose contents, contribute to the superior quality of D. brandisii shoots. Integrating genomic, transcriptomic, and metabolomic datasets provided a solid foundation for enhancing bamboo shoot quality and developing efficient gene-editing techniques. This study should facilitate research on D. brandisii and enhance its use as a food source and wood material by providing crucial genomic resources.

Effect of liquid nitrogen spray quick-freezing technology on the quality of bamboo shoots, Dendrocalamus brandisii from Yunnan Province, China

This study explored the effects of liquid nitrogen spray quick-freezing (LNF) on the quality of fresh bamboo shoots. The results showed that the low temperature damaged bamboo shoots and caused a loss of nutrients after freezing. Notably, liquid nitrogen spray quick-freezing technology significantly improved the freezing efficiency and quality of the sample compared with the refrigerator freezing at −20 °C. The bamboo shoots that underwent LNF at −80 °C (LNF-80 °C) suggested the best results, with a 92.73% reduction in passing the zone of maximum ice crystal formation time, 60.23% reduction in thawing loss, 79.32% increase in hardness and 33.61% decrease in peroxidase activity compared that of refrigerator freezing at −20 °C. On the nutrients, the total amino acid content in the LNF-80 °C bamboo shoots (133.31 mg/100 g) increased by 25.50% compared with that in bamboo shoots that were slowly frozen (835.24 mg/100 g). Furthermore, the LNF-80 °C group samples had the most uniform water distribution, and the microstructure of this sample was the least damaged by ice crystals. These results show that LNF technology has great potential to improve the quality of frozen fruits and vegetables.

Forest Canopy Structures and Bamboo Rhizome Internodes Impact the Appearance Quality of Bamboo Shoots

Bamboo shoots are a healthy vegetable with significant commercial value, and their appearance quality is a key factor influencing consumer preference and market pricing. Their growth characteristics—after being unearthed, they maintain basal diameter while rapidly growing in height—affect the taste and nutritional quality. However, little attention has been given to the impact of bamboo forest management on shoot appearance. Therefore, this study addressed this research gap through a comprehensive investigation across three types of bamboo forests: evergreen broad-leaved forest (EBF), evergreen deciduous broad-leaved mixed forest (MBF), and pure bamboo forest (PBF). In addition, we further assessed factors that potentially affect the appearance quality of bamboo shoots, including canopy structures, understory light factors and understory soil factors, mother bamboo factors, and shoot internal factors (pigments and cells). The basal diameters of shoots in PBF and MBF were 1.89 cm and 1.97 cm, respectively, which were significantly larger than those in EBF by 0.27 cm and 0.35 cm, respectively. The linear mixed effect model identified the number of bamboo rhizome internodes and the chlorophyll a content as primary factors influencing basal diameter thickening and elongation growth of shoots, respectively. In addition, increasing the bamboo canopy and mean leaf angle reduced the chlorophyll a content and increased the carotenoid content, thereby benefiting the improvement in or maintenance of the taste and quality of shoots. This study highlighted that increasing the number of bamboo rhizome internodes, bamboo canopy, and mean leaf angle is helpful to improve the appearance quality of shoots. These findings offer a scientific foundation for bamboo forest management, contributing to both ecological sustainability and economic benefits.

Using IoT Smart Basketball and Wristband Motion Data to Quantitatively Evaluate Action Indicators for Basketball Shooting

Traditional approaches to improving basketball players’ shooting skills rely on coaches’ experience in adjusting players’ biomechanical motions. However, such an approach cannot provide specific instructions or facilitate immediate feedback for improvement of the shooting motion. In this article, a method is presented to quantitatively evaluate four key action indicators of shooting basketballs using a machine‐learning model based on Bayesian optimization of a light gradient boosting machine (LightGBM). Important motion data for the model are collected by micro‐inertial measurement units embedded in a wrist motion sensor and an internet of things (IoT) smart basketball. Basketball shooting motion data are collected from 16 subjects and used for model training and data testing, and four important action indicators that influence the shot quality are selected for quantitative assessment. The LightGBM model is then developed for the regression prediction of the four action indicators of shooting. In the results, it is indicated that for an individual player, the highest correlation scores of the four indexes range from 97.6% to 99.3%. The proposed approach for quantitatively assessing shooting indexes can provide objective and data‐based guidance to improve players’ shooting performance. Foreseeably, the prediction model can be embedded into a chip of a wearable device to evaluate the real‐time shot quality quantitatively.

Correlation and Path Analysis for Growth, Yield, Quality and Incidence of Shoot and Fruit Borer in Brinjal (Solanum melongena L.)

The study was conducted using sixty brinjal genotypes to evaluate yield quality and incidence of shoot and fruit borer with an objective to study the correlation and path coefficient analysis. The majority of the time, genotypic correlations were higher than phenotypic correlations, indicating that the attribute is highly heritable. At the genotypic level, fruit yield per plant significantly correlated positively with the number of fruits per plant, the weight of infested fruit, the girth of fruit, the height of the plant, the number of primary branches per plant, the proportion of medium-styled flowers, the proportion of long-styled flowers, and the length of fruits. It an exhibited significant negative correlation with SFB fruit infestation as well as SFB shoot infestation both at phenotypic and genotypic levels. Path coefficient revealed that fruits per plant showed a high and positive direct effect on yield followed by fruit weight, long -styled flowers, medium -styled flowers, and days to first harvest. Direct selection for these traits would be rewarding for improvement in the fruit yield per plant.

Integrative analysis of metabolome and transcriptome reveals the different metabolite biosynthesis profiles related to palatability in winter and spring shoot in moso bamboo

Moso bamboo winter shoot has good taste and rich nutritional value. To reveal the causes and regulatory mechanism of palatability deterioration from winter to spring shoot, a conjoint analysis of metabolome and transcriptome was conducted on winter and spring shoots of moso bamboo. Totally 909 metabolites were characterized for the first time. The significant increase of hydrolyzed tannin content intensified the bitterness of spring shoot, which was positively regulated by key metabolite (gallic acid) and genes (DAHPS, DHQS, DHQ, SDH) in the biosynthesis pathway of hydrolyzed tannin. The accumulation of lignified components enhanced the roughness of spring shoot, which was closely connected with the significant changes of important metabolites (cinnamic acid, ferulic acid, UDP-glucose and UDP-xylose) and up-regulation of most enzyme genes involved in the biosynthesis pathways of lignin, cellulose and hemicellulose. The present study provides theoretical support for understanding palatability transition and directional improvement of edible quality of moso bamboo shoots.

Comparative Study of Bolting Adaptability between 60Co-Induced Rape and Its Original Material

In southern China, the fresh shoots of rape are used as a high-quality seasonal vegetable owing to their pleasant taste. In this study, we investigated the taste and quality of fresh shoots of Fanmingyoutai, which was derived from WH23 by 60Co mutation. WH23 was used as a control (CK). Physiological indexes, transcriptome analyses, and metabolomics analyses between Fanmingyoutai and CK were studied and the related key differential genes were identified. The results showed that the glucosinolate content of Fanmingyoutai seeds was 51.14% lower than that of CK, and the contents of soluble sugar and vitamin C in the fresh shoots of Fanmingyoutai were 2.1 times and 1.4 times higher, respectively, than CK. Using transcriptome analyses, we identified that the differential genes were involved in glycan biosynthesis and metabolism, energy metabolism, carbohydrate metabolism, and the metabolism of cofactors and vitamins. Metabolomics analyses demonstrated that the contents of sucrose and D-fructose in the fresh shoots of Fanmingyoutai were 1.22 times and 1.15 times higher, respectively, than those in CK. Using qRT-PCR analyses, the expression of SWEET17, STP5, and GSL in the fresh shoots and leaves of Fanmingyoutai was two times higher than that in CK. SWEET17 (involved in sugar production and transport), STP5 (involved in monosaccharide transport), and GSL (involved in glucosinolate accumulation) may be the key functional genes. We concluded that the low glucosinolate content and high sucrose and D-fructose contents may be the main factors affecting the taste of fresh shoots of Fanmingyoutai and CK; SWEET17, STP5, and GSL may be the key related genes. This research provides a reference for the breeding and molecular mechanisms of new edible rape varieties.

Effects of cold plasma treatment on maintaining the quality of fresh-cut bamboo shoots during shelf-life storage

Cold plasma (CP) is an emerging preservation technology that involves minimal processing and retains the freshness of fresh-cut products. In this study, we applied CP (50 kV, 30 s) to evaluate the potential use on the freshly cut Ma bamboo (Dendrocalamus latiflorus Munro) shoots using physicochemical, headspace-solid phase microextraction-gas chromatography-mass spectrometry (HS-SPME/GC-MS), 16S rRNA gene sequencing, and metabolomic analyses. The results showed that the firmness of CP treated bamboo was 57% higher than control groups, and CP treatment could effectively inhibit yellowing for up to 5 day at 25 °C. Six key flavor compounds (odor activity value (OAV) > 1) were detected. 4-Hydroxy-benzaldehyde was the most abundant compound, accounting for 45.53%–78.46% in bamboo shoots. Aldehyde, ketone, and ester contents increased significantly, whereas alcohols and alkenes were reduced by CP treatment. CP induced higher microorganism community diversity within four days. Freshly cut bamboo shoots had a high concentration of metabolites that were closely related to the lignin biosynthesis. Through the accumulating of stress-related hormones, fatty acids, and flavonoids, CP boosted the response to external stress. This method offers a new approach to preserve the quality of freshly cut bamboo shoots.

Comparative characteristics of the young football players of different game roles technical and physical fitness at the specialized basic training stage

Rationale and purpose&#x0D; Differentiations of physical loads for athletes of different game roles in football are very impotent. The purpose of this research is to reveal the peculiarities of the technical and physical fitness of young football players of 15 years of age in various playing roles.&#x0D; Material and Methods &#x0D; 51 young football players were examined according to the level of physical and technical preparation, including 9 goalkeepers, 12 defenders, 15 midfielders, and 15 attackers. The following indicators were measured: body length, body weight, running time for 15 m, 30 m, 60 m, shuttle running time 5X20 m, heart rate at rest, heart rate after shuttle running, ball juggling (number of kicks with the ball before losing it), time of the ball in a certain area of the goal. For the comparative analysis of the indicators of football players of different playing roles, the dispersion analysis of the comparison of several unrelated samples according to Duncan’s multiple comparisons criterion was used.&#x0D; Results &#x0D; Based on the results of variance analysis using Duncan’s multiple comparisons criterion, it was established that goalkeepers have the smallest body length (p&lt;0.05) and the highest indicator of technical readiness “Ball hit per range, m” (p&lt;0.05). Defenders and midfielders showed the best indicators of speed endurance according to the indicators of the test “Time of execution of the shuttle run 5X20 m, s” (p&lt;0.05). At the same time, their heart rate after performing this test is significantly lower than that of attackers (p&lt;0.001). Also, attackers and defenders have significantly higher indicators of speed abilities compared to representatives of other playing roles according to the results of the “30 m, s” test (p&lt;0.001). In addition, defenders and midfielders, and forwards have significantly higher speed abilities according to the results of the “Run 15 m, s” and “Run 60 m, s” tests compared to goalkeepers (p&lt;0.001). Defenders can keep the ball on their feet the longest (p&lt;0.001) and forwards can perform the most kicks (juggling the ball) (p&lt;0.01). Attackers and midfielders have the highest quality of shots on goal compared to goalkeepers and defenders (p&lt;0.05).&#x0D; Conclusions &#x0D; As a result of the cluster analysis, the functions of volleyball players were clarified and the players most similar to each other in terms of their preparation structure were identified, which sometimes causes some difficulties for coaches when working with athletes with implicitly expressed game functions. Individual profiles of players were compiled and individual recommendations for training volleyball players were developed based on the individual values of readiness factors and the results of cluster analysis.

Use of Liquid Culture with the ElecTIS Bioreactor for Faster Recovery of Blackberry (Rubus fruticosus L.) Shoots from Conservation at 4 °C

ElecTIS is a new single container bioreactor which does not require forced air blowing, instead making the culture material mobile and the liquid medium stationary. The timed up-and-down movement of the basket containing the shoot culture ensures periodic contact with the liquid medium positioned at the base of the container. In this study we tested for the first time its use in the recovery of blackberry shoot cultures (Rubus fruticosus L., cvs Thornfree and Chester), coming from 5 months of slow growth storage (SGS), at 4 °C and in the dark. The shoot recovery at standard culture conditions was performed on two different types of ElecTIS, i.e., one with a smaller basket (ElecTISS, 234 cm2 of culture area), and one with a large basket (ElecTISL, 336 cm2), comparing the culture in TIS (cycle of 8 min every 6 h, equal to 32 min/day) with the traditional one in a gelled medium in glass jars (500 cc). After each one of the three 4-week subcultures, the shoot growth parameters and the relative growth rate highlighted a clear superiority of ElecTIS in promoting the recovery of shoot cultures coming from SGS. The analyses of chlorophyll content and stoma functionality confirmed the superior quality of shoots cultured in the ElecTIS bioreactor, and these shoots were afterwards easily rooted and acclimatized ex vitro.