An investigation was carried out at farm of College of Agriculture Nagpur Maharashtra during, 2011-2012 to evaluate the soil fertility and quality of safflower. The experiment was laid out in Randomized Block Design (RBD) with three replication and nine treatments combination of FYM in Kharif season, biofertilizer and inorganic fertilizer at the time of sowing in rabi season using variety AKS/S-41. The result indicated that various physical properties as bulk density were decreases with increasing level of RDF with seed treatment Azospirillum + PSB and maximum water holding capacity increased with increasing level of RDF alone or in combination with Azospirillum + PSB. The changes in pH due to fertilizer application were significant. The changes in EC due to fertilizer (organic and inorganic) application were non-significant. The highest organic carbon, available NPK and S was recorded in the treatment of 100% RDF + Azospirillum + PSB and available Fe, Mn, Zn and Cu was recorded significantly highest in same treatment.