Rooster Sperm Quality Research Articles

Unveiling the Potential of Aloe vera Gel Supplementation in a Cooling Extender: A Breakthrough in Enhancing Rooster Sperm Quality and Fertility Ability.

The cooling of semen storage at 5 °C from a Thai native rooster (Pradu Hang Dum), supplemented with herbs possessing antioxidant properties, provided limited research. This study was conducted to evaluate the efficiency of Aloe vera (AV) gel supplementation at various levels on the quality of cooled semen and subsequent fertility after artificial insemination. Sixty-four chickens had semen pooled, diluted, and supplemented with different levels of AV gel (0% as control, 0.25%, 0.50%, 1.0%, 2.5%, 5.0%, 10%, and 20%), and then stored for 72 h. In Experiment 1, semen quality, malondialdehyde (MDA) levels, and pH values were assessed at 0, 24, 48, and 72 h after storage. Experiment 2 assessed fertility potential using the most effective cooled storage semen from Experiment 1. Results showed a decrease in semen quality with prolonged storage time (p < 0.001). The highest semen quality was observed in the group supplemented with 1.0% AV gel (p < 0.001), whereas the lowest was noted in the 20% AV gel group (p < 0.001). Furthermore, the 1.0% AV gel group exhibited the highest semen quality at 24, 48, and 72 h of storage. The evaluation of fertility and hatchability rates revealed a statistically significant improvement in fertility potential (p < 0.05) in the group supplemented with 1.0% AV gel. In summary, this study represents the first investigation of stored Thai native rooster semen using a semen extender supplemented with Aloe vera gel at 5 °C, demonstrating its efficacy for storage up to 72 h. The addition of 1% AV gel was recommended as an antioxidant supplementation during the semen storage process at 5 °C to enhance semen quality and fertility rates.

Characteristics of production, reproduction, and sperm quality in Tukong, a rumpless chicken breed from Indonesia

Information on the production and reproductive performances of local chickens is crucial for the development of genetic improvement as well as for fostering poultry industry resilience. With limited data available, this study aimed to analyse the production and reproduction of Tukong chickens, a variety of chicken known for its rumpless, originating from West Kalimantan, Indonesia. Two hundred five Tukong chickens, comprising 153 hens and 52 roosters, were raised under intensive management for the study. The collected data included egg production, reproduction traits, and rooster sperm quality. The average body weight of the hens was 1140.55 ± 260.78 g, while the roosters weighed 1434.16 ± 387.43 g. Other production traits, such as first egg weight, number of eggs per clutch, number of clutches per year, average egg weight, and hatching weight, were lower compared to other local chicken breeds in Indonesia. The female chicken had its first egg laid at 241.64 ± 24.65 d, with a fertility rate of 75.34 ± 1.43% and a hatchability rate of 83.05 ± 1.28%. All parameters related to sperm quality in Tukong chickens fell within normal ranges. The findings of this study suggest opportunities for furthering breeding improvements for the Tukong chicken.

Effects of hydroxytyrosol on post-thaw quality of rooster sperm.

Semen cryopreservation is one of the most important reproduction techniques in the livestock and poultry industry. Cryopreservation induces cold stress, generating reactive oxygen species (ROS) and oxidative stress causing structural and biochemical damages in sperm. In this study, we evaluated the effects of the hydroxytyrosol (HT), as an antioxidant, at the concentrations of 0, 25, 50, and 100 μg/mL on post-thaw semen quality metrics in rooster. Semen samples were collected twice a week from 10 roosters (29 weeks), processed and frozen according to experimental groups. Different quality parameters, including total motility, progressive motility, viability, morphology, membrane integrity, and malondialdehyde were measured after thawing. Results showed that 25 and 50 μg/mL of HT produced the highest percentage of total motility (51.01 ± 2.19 and 50.15 ± 2.19, respectively) and progressive motility (35.74 ± 1.34 and 35.15 ± 1.34, respectively), membrane integrity (48.00 ± 2.18 and 46.75 ± 2.18, respectively) as well as viability (53.00 ± 2.17 and 52.50 ± 2.17, respectively) compared with the other groups (p < .05). The group with 25 μg/mL of HT showed the lowest significant (p < .05) MDA concentration (1.81 ± 0.25). Our results showed that the effect of HT was not dose-dependent and optimum concentration of HT could improve functional parameters of rooster sperm after freezing-thawing. These findings suggest that HT may have protective effects on the rooster sperm during the freezing-thawing process.

Protective Effect of N-Acetylcysteine on Rooster Semen Cryopreservation.

Cryopreservation of avian semen is a useful reproductive technique in the poultry industry. However, during cooling, elevated reactive oxygen species (ROS) levels have destructive effects on both quality and function of thawed sperm. The aim of the current study is to investigate the antioxidant effects of N-acetylcysteine (NAC) during rooster semen cryopreservation. Semen samples were collected from ten Ross 308 broiler breeder roosters (32 weeks) and mixed. The mixed samples were divided into five equal parts and cryopreserved in Lake Buffer extender that contained different concentrations (0, 0.01, 0.1, 1, and 10 mM) of NAC. The optimum concentration of NAC was determined based on quality parameters of mobility, viability, membrane integrity, acrosome integrity, lipid peroxidation, and mitochondrial membrane potential after the freeze-thaw process. There was a higher percentage (p < 0.05) of total motility (TM) (60.9 ± 2.4%) and progressive motility (PM) (35.6 ± 1.9%) observed with the NAC-0.1 group compared to the other groups. Significantly higher percentages of viability (74.4 ± 2.3% and 71 ± 2.3%), membrane integrity (76.4 ± 1.5% and 74.7 ± 1.5%) and mitochondrial membrane potential (67.1 ± 1.6% and 66.3 ± 1.6%) were observed in the NAC-0.1 and NAC-1 groups compared to the other frozen groups (p < 0.05). The lowest percentage of lipid peroxidation and nonviable sperm was found in the NAC-0.1 and NAC-1 groups compared to the other groups (p < 0.05). The average path velocity (VAP), straight line velocity (VSL), curvilinear velocity (VCL), and acrosome integrity, were not affected by different concentrations of NAC in the thawed sperm (p > 0.05). Both NAC-0.1 and NAC-1 appear to be beneficial for maintaining the quality of rooster sperm after thawing.

Zinc oxide nanoparticles preserve the quality and fertility potential of rooster sperm during the cryopreservation process.

Sperm cryopreservation is one of the main methods for preserving rooster sperm for artificial insemination (AI) in commercial flocks. Yet, rooster sperm is extremely susceptible to reactive oxygen species (ROS) produced during the freezing process. Oxidative stress could be prevented by using nanoparticles containing antioxidants. The present study was conducted to investigate the effect of zinc oxide nanoparticles (ZnONP) in rooster semen freezing extender on quality parameters and fertility potential. For this aim, semen samples were collected and diluted in Lake extenders as follows: control: Lake without ZnONP, ZnO100: Lake with 100-μg zinc oxide (ZnO), ZnONP50: Lake with 50-μg ZnONP, ZnONP100: Lake with 100-μg ZnONP and ZnONP200: Lake with 200-μg ZnONP. After freezing and thawing, sperm motility, viability, membrane integrity, morphology, mitochondrial activity, acrosome integrity, DNA fragmentation, lipid peroxidation and ROS, as well as fertility and hatchability were assessed. According to the current results, higher rates of motility, membrane integrity, mitochondrial activity, acrosome integrity and live cells were detected in the ZnO100, ZnONP50 and ZnONP100 groups compared to other groups (p ≤ .05). Yet, the percentage of dead cells, DNA fragmentation, lipid peroxidation and ROS levels were lower in the mentioned groups (p ≤ .05). Furthermore, a higher percentage of fertility was observed in the ZnO100 and ZnONP100 groups than in the control group (p ≤ .05). In conclusion, the use of 100-μg ZnO and 50- to 100-μg ZnONP represents a valuable and safe additive material that could be used to improve the quality and fertility potential of rooster sperm under cryopreservation conditions.

The Quality of KUB Rooster Sperm During Cryopreservation in Extender with Genistein and Glutathione Supplementation

The Quality of KUB Rooster Sperm During Cryopreservation in Extender with Genistein and Glutathione Supplementation

The effect of the mitochondrial-directed antioxidant Mito-TEMPO on the quality of rooster sperm in the cryopreservation and lyophilization cycle

In reproductive technologies applied in animal husbandry, using lyophilized semen is only possible with ICSI technology, due to complete loss sperm motility. However, the anatomical structure’s peculiarities and development of avian oocyte not allows use this technology. Strategies aimed at preserving kinetic apparatus of lyophilized sperm are the most relevant for birds. Increasing mitochondrial activity of cells due to its connection with energy metabolism and sperm motility may be one of the ways to solve this problem. However, similar studies on freeze-dried bird semen haven’t been conducted on this moment. The present study assessed effect of mitochondria-targeted antioxidant Mito-TEMPO on viability, mitochondria’s membrane potential and generation of intracellular hydrogen peroxide by rooster spermatozoa in a cryopreservation and lyophilization cycle. Proportion of cells with intact plasma membrane in lyophilized semen was higher using extenders containing an antioxidant. Addition of Mito-TEMPO at concentration of 40 µM to diluents T-20 and T-30 for the purpose of cryopreservation of semen with subsequent freezedrying allowed to increase proportion of cells with intact plasma membrane in lyophilized semen in comparison to control diluents (6.12 ± 0.11 % versus 10.19±0.46% and 13.19±1.30% versus 15.23±1.13%, respectively, p˂0.05). Effective concentration of Mito-TEMPO in diluents intended only for semen cryopreservation was significantly lower - 5 µM. Proportion of cells with intact plasma membrane was 47,97±0,64% versus 55,85±1,69% and 47,25±2,97% versus 63,21±1,78%, respectively, p˂0, 05. Dose-dependent effect of trehalose in cryoprotective diluents has been proven.

Supplementation of Freezing Medium with Ginseng Improves Rooster Sperm Quality and Fertility Relative to Free Radicals and Antioxidant Enzymes.

To the best of our knowledge, this study is the first to determine the effect of ginseng as an antioxidant supplement in freezing extenders on the quality of cryopreserved rooster semen. Semen samples were collected from 40 Thai native roosters (Pradu Hang Dum) using the dorso-abdominal massage method and then pooled and divided into five groups according to the concentrations of ginseng supplementation (0, 0.25, 0.50, 0.75, and 1 mg/mL) in a freezing extender. The semen suspensions were loaded into a medium straw and cryopreserved using the liquid nitrogen vapor method. The post-thaw semen was evaluated for sperm quality (sperm motility and membrane integrity), seminal plasma characteristics (lipid peroxidation, superoxide dismutase [SOD], catalase [CAT], and glutathione peroxidase [GPx]), and fertility. The results showed that ginseng extract supplementation at 0.25 mg/mL yielded the highest total motility, progressive motility, and membrane integrity (59.47%, 30.82%, and 48.30%, respectively; p < 0.05) in cryopreserved rooster semen. Higher malondialdehyde concentrations were observed in the control group than in the other groups (p < 0.05). SOD, CAT, and GPx increased compared with those in the control group (p < 0.05). The results showed that the fertility rate with 0.25 mg/mL of ginseng was higher than that of the control group (62.80% vs. 46.28%: p < 0.05). In conclusion, supplementation with 0.25 mg/mL of ginseng is recommended as an alternative component to the freezing extender to improve rooster semen cryopreservation.

Increasing sperm production and improving cryosurvival of semen in aged roosters after diet-supplemented with selenium in Thai native chickens.

Aging roosters typically exhibit subfertility with decreasing semen quality, whereas Thai native roosters reared in rural areas were raised for a longer duration than their usual lifespan. The present study therefore aimed to assess the effect of selenium supplementation as an antioxidative substance in diets to improve the semen cryopreservation of aged roosters. Semen samples were collected from young (n=20) and aged (n=20) Thai native roosters (Pradu Hang Dum) at 36 and 105 weeks of age when starting the experiment, respectively. They were fed diets in non-supplemented or supplemented selenium (0.75 ppm). Fresh semen quality and lipid peroxidation of fresh semen was evaluated before cryopreservation using the traditional liquid nitrogen vapor method. Post-thaw sperm quality and fertility potential were determined. Advancing age is unrelated to decreasing fresh semen quality (p > 0.05). However, lipid peroxidation in rooster semen depended on age, and the malondialdehyde (MDA) concentration increased in aged roosters (p < 0.05). Selenium supplementation in diets significantly decreased the MDA concentration and increased the sperm concentration (p < 0.05). In contrast, cryopreserved semen was affected by advancing rooster age, and selenium influenced sperm quality (p < 0.05). Younger roosters had higher post-thaw sperm quality and fertility potential than aged roosters (p < 0.05). Likewise, diet selenium supplements improved post-thaw sperm quality and fertility compared with the non-supplement group. Rooster's age does not influence the rooster sperm quality of fresh semen, while sperm cryotolerance and fertility were greater in young roosters than in aged roosters. However, those of aged roosters could be improved by dietary selenium supplementation.

Preservation of the quality and fertility potential of post-thawed rooster sperm using MitoQ

Cryopreservation of rooster spermatozoa is an efficient procedure to spread qualified semen samples for reproductive goals in commercial flocks, but the freeze-thawing process reduces the quality and fertility potential of post-thawed sperm cells. This study was aimed to investigate the effect of the mitochondria-targeted antioxidant MitoQ on rooster sperm quality and fertility potential preservation during freeze-thawing process. Semen samples were collected and diluted in the Lake medium, assigned into five equal aliquots, supplemented with 0, 1, 10, 100 and 1000 nM MitoQ, and cryopreserved in liquid nitrogen. After thawing, sperm motility, membrane functionality, abnormal morphology, mitochondria active potential, acrosome integrity, viability, apoptosis status, lipid peroxidation, DNA fragmentation, ROS concentration and fertility potential were evaluated. According to the results, freezing extender supplementation with 10 and 100 nM MitoQ presented higher (P ≤ 0.05) total motility, progressive motility, average path velocity, membrane functionality, mitochondria active potential, acrosome integrity and viability compared to the other groups. On the other hand, lipid peroxidation, apoptosis rate, DNA fragmentation and ROS concentration were lower (P ≤ 0.05) in groups received 10 and 100 nM MitoQ compared to other groups. Moreover, fertility rate was higher in groups received 10 and 100 nM MitoQ compared to control group. Therefore, MitoQ is able to preserve quality parameters and fertility potential of post-thawed spermatozoa in rooster and it could be an effective additive for supplementation of rooster's semen cryopreservation medium during reproductive programs.

The Quality of KUB Rooster Sperm with Vitamin E and C Supplementation During Room Temperature Storage

Antioxidant supplementation to semen extenders can help to maintain rooster sperm quality. This study determined how vitamins E and C affected the quality of KUB sperm at room temperature. Semen collection from 12 KUB roosters used dorso abdominal massage method, twice a week. Semen samples were then diluted using LR-EY extender with three treatments: LR-EY without antioxidant as the control, LR-EY supplemented 0.05 mg/ml vitamin E, and 0.2 mg/ml vitamin C. The diluted semen samples were then stored at room temperature, then motility and viability were examined at 0, 1st, 2nd, and 4th hour. The results showed that the percentage of motility between treatment groups and time points differed not significantly (P>0.05). However, vitamin E supplementation had a significant (P<0,05) effect on the percentage of viability in the 2nd and 4th hour. Supplementation of vitamin E 0.05 mg/ml was able to maintain the viability of KUB rooster sperm at 2nd hour (88.75 ± 1.50%) and 4th hour (84.75 ± 2.63%). It can be concluded that vitamin C cannot maintain sperm quality significantly, but vitamin E can preserve the viability of KUB rooster sperm significantly at room temperature.

Curcumin Attenuates Damage to Rooster Spermatozoa Exposed to Selected Uropathogens.

Artificial insemination, as an essential pillar of the modern poultry industry, primarily depends on the quality of semen collected from stud roosters. Since the collection and storage of ejaculates is not a sterile process, antimicrobial agents have become essential supplements to semen extenders. While the use of traditional antibiotics has been challenged because of rising bacterial resistance, natural biomolecules represent an appealing alternative because of their antibacterial and antioxidant properties. As such, this study strived to compare the effects of 50 μmol/L curcumin (CUR) with 31.2 µg/mL kanamycin (KAN) as a conventional antibiotic on rooster sperm quality in the presence of Salmonella enterica, Escherichia coli and Pseudomonas aeruginosa. Changes in sperm structural integrity and functional activity were monitored at 2 and 24 h of culture. Computer-assisted semen analysis revealed significant sperm motility preservation following treatment with KAN, particularly in the case of Salmonella enterica and Pseudomonas aeruginosa (p &lt; 0.001) after 24 h. On the other hand, CUR was more effective in opposing ROS overproduction by all bacteria (p &lt; 0.05), as determined by luminol-based luminometry, and maintained sperm mitochondrial activity (p &lt; 0.001 in the case of Salmonella enterica; p &lt; 0.05 with respect to Escherichia coli and Pseudomonas aeruginosa), as assessed by the fluorometric JC-1 assay. The TUNEL assay revealed that CUR readily preserved the DNA integrity of rooster sperm exposed to Salmonella enterica (p &lt; 0.01) and Escherichia coli (p &lt; 0.001). The bacteriological analysis showed higher efficiency of KAN in preventing the growth of all selected bacterial species (p &lt; 0.0001) as opposed to CUR. In conclusion, CUR provided protection to rooster spermatozoa against alterations caused by uropathogens, most likely through its antioxidant activity. Hence, CUR supplementation to poultry semen extenders in combination with properly selected antibacterial substances may become an interesting strategy in the management of bacterial contamination during semen storage.

APPLICATION OF MULTIVARIATE ANALYSIS: TOWARD IMPROVEMENT OF ROOSTER SEMEN QUALITY ASSESSMENT

Sperm cryopreservation is an important tool for storage of genetic material of animal species. Therefore, the aim of this study was to assess the association between extender and cryoprotective agents (CPAs) used in the term of sperm quality parameters. In our study, the comparison of two extenders (Kobidil+ (K+) and saline (S)) and their combinations with CPAs such as dimethyl sulfoxide (DMSO) or glycerol (GL) on the sperm motility and viability was analyzed. For detailed assessment of association between extenders with CPAs and sperm quality parameters of frozen/thawed semen, multivariate analysis was used. The obtained data showed that the both extenders with specific CPAs significantly affect the motility as well as the viability parameters. GL/K+ (p-value=0.0168) had effect on the progressive motility, whereas GL/S (p-value=0.0174) influenced the total motility and velocity curved line parameters. Moreover, DMSO/K+ (p-value=0.0346) had higher effect on the proportion of dead and apoptotic sperm compared to DMSO/S (p-value=0.036), which had higher impact on the proportion of acrosome damaged sperm. Future studies might benefit from these findings to establish the associations of these subpopulations with other rooster sperm quality parameters and thus improve the sperm quality. Association between the values can help us to better understand the effect of extenders and CPAs on the semen quality parameters.

Effect of tempol and straw size on rooster sperm quality and fertility after post-thawing

The aim of the present study was to investigate the effects of tempol and straw size on rooster sperm post-thaw quality and fertility. Rooster semen was cryopreserved in Lake extender containing 0 (control), 5, 10, 15 and 20 μM tempol (in two different straw size, 0.25 and 0.5). The percentage of total and progressive sperm motility, VAP and VSL increased in the 10 µM tempol group. Moreover, 10 µM tempol led to lower ROS compared to other groups. The lowest percentage of apoptotic-like changes was detected when the extender was treated with 10 µM of tempol. The minimum ROS was observed in the group treated with 0.5 straw size. Straw size did not have any significantly effect on GPx and SOD activities and TAC of frozen-thawed sperm. The highest significant percentage of fertility and hatching rate were observed in 10 µM of tempol. The results of the present study showed that supplementation of the Lake cryopreservation medium with 10 µM tempol improved cryo-survival. Also, the results of the present study suggested that Lake cryopreservation medium with 0.5-ml straw may perhaps be an appropriate method to improve the quality and fertility post-thawed rooster sperm.

Improving the Quality of Rooster Sperm during Storage at 4 °C by adding Quercetin in the form of nano-Liposomes and NLC in a Diluting Medium

Improving the Quality of Rooster Sperm during Storage at 4 °C by adding Quercetin in the form of nano-Liposomes and NLC in a Diluting Medium

Supplementation of sperm cooling medium with Zinc and Zinc oxide nanoparticles preserves rooster sperm quality and fertility potential

Supplementation of sperm cooling medium with helpful additives is a reasonable method to conserve sperm fertility potential during cooling storage process. This study was aimed to determine the effect of sperm cooling medium supplementation with Zinc and Zinc oxide nanoparticles (NZn and NZnO) on rooster semen quality and fertility efficiency during storage periods. Semen samples were diluted in the Lake medium and assigned into five equal aliquots. The first was Control group and the other groups received 50 μg/ml NZn, 50 μg/ml NZnO, 100 μg/ml NZn and 100 μg/ml NZnO. Then, the samples were cooled at 5 °C and conserved up to 45 h. Total motility, progressive motility, mitochondrial activity, viability, membrane integrity and lipid peroxidation of samples were analyzed during 0, 22 and 45 h post-cooling. Artificial insemination was also performed using 22- hrs cooled semen. No difference was found among groups during quality evaluations at 0 h storage. Extender supplementation with 100 μg/ml NZn and NZnO presented higher (P ≤ 0.05) total motility, progressive motility, mitochondrial activity, viability, membrane integrity and lower lipid peroxidation compared to other groups during 22 and 45 h cooling storage. Fertility rate of 22-h cooled-stored semen samples was higher (P ≤ 0.05) in groups contained 100 μg/ml NZn and NZnO compared to the Control group. In conclusion, addition of 100 μg/ml NZn and NZnO to the sperm storage medium could be introduced as an effective method to preserve rooster semen quality during cooling storage period.

Effect of vitamin E supplementation on chicken sperm quality: A meta-analysis.

Background and Aim:Among several factors, the sperm quality of poultry is affected by the rooster’s body size and the availability of antioxidants like vitamin E. This study aimed to determine the effect of dietary vitamin E supplementation on rooster sperm quality through a meta-analysis.Materials and Methods:After verification and evaluation, a total of 19 articles were included in this study. Data, including dietary vitamin E, semen volume, concentration, total sperm cells, pH, motility, viability, percentage of dead and abnormal sperm, vitamin E sperm content, malondialdehyde (MDA) content, and testosterone levels, were tabulated in a database; these were subsequently analyzed using mixed modeling with vitamin E dose as a fixed effect and study identity as a random effect.Results:Dietary supplementation level of vitamin E significantly (p<0.001) affected sperm concentration, significantly affected motility (p<0.001), significantly affected sperm vitamin E (p<0.001), significantly affected viability (p<0.001), and significantly affected chicken sperm fertility (p=0.001). Vitamin E administration also significantly reduced the number of sperm cell deaths (p<0.001); however, increased dietary levels of vitamin E did not affect semen volume (p=0.853), pH (p=0.951), MDA (p=0.542), the percentage of abnormal sperm cells (p=0.343), nor testosterone levels (p=0.063).Conclusion:Dietary vitamin E supplementation is recommended for male chickens since it generally enhances the quality of their sperm.

Comparing the effect of rooster semen extender supplemented with gamma-oryzanol and its nano form on post-thaw sperm quality and fertility

Antioxidant nanoparticles include the potential for improving sperm cryopreservation. The aim of performing this study was to evaluate the effects of gamma-oryzanol (GO) at 0 (C) (control group), 20 (GO20), 40 (GO40), 60 (GO60), 80 (GO80), and 100 (GO100) µM and gamma-oryzanol nanoparticles (GON) at 0 (CN), 20 (GON20), 40 (GON40), 60 (GO60), 80 (GON80), and 100 (GON100) µM on post-thawed sperm quality and fertility of rooster sperm. Sperm motility, plasma membrane integrity, total abnormality, mitochondrial activity (Rhodamine 123), apoptotic features (Annexin V/Propidium iodide), reactive oxygen species (ROS) production, ATP content and the fertility and hatchability were evaluated after thawing. Total motility in GON60 and GON80 were significantly higher compared to control groups (C and CN). GON80 showed the greatest percentages of progressive motilities. When GO80, GON60, and GON80 were added to the cryopreservation medium, the plasma membrane functionality of the semen samples improved. The minimum abnormality of spermatozoa is observed in the group treated with GON80. The groups treated with GON60 and GON80 had greater (P < 0.05) mitochondrial activity. The level of sperm ROS after cryopreservation was significantly lower in GON60 and GON80 groups. Live sperm was significantly higher (P < 0.05) in GON60 and GON80 group compared to other groups. GON60 and GON80 groups also led to the lowest significant percentage of apoptosis-like change sperm. Greater fertility percentages were observed (P < 0.05) when sperm were stored in extenders treated with GON60 and GON80. GON80 resulted in significantly improved hatched eggs compared to C, GO60, GO180 and CN. In conclusion, supplementation of Lake extender with 60 and 80 µM gamma-oryzanol nanoparticles could be a proper process to improve freeze-thawing rooster sperm quality leading to better freeze/thaw characteristics.

Effects of Rutin On The Quality of Rooster Sperm During Cryopreservation

Effects of Rutin On The Quality of Rooster Sperm During Cryopreservation

Effects of Melatonin On The Rooster Sperm Quality During Cryopreservation

Effects of Melatonin On The Rooster Sperm Quality During Cryopreservation