Current processes in the field of public healthcare — optimizing public healthcare institutions, formation of the market of paid medical services, changes in the legislation and in the requirements for qualification of healthcare workers — require new approaches in management. Today is needed not only redistribution of the volumes of medical assistance between different organizations, but also a review of the healthcare infrastructure, which should meet the needs of the population and ensure the quality of medical assistance. Introduction of Professional standards this year and transition to accreditation of all doctors and nurses directly affect the requirements for qualifications of medical workers. All this requires development of targeted training system in higher education and internship, creating a nationwide database of vacancies in healthcare organizations. The formats of work themselves and the requirements for employees are changing. The main task is to eliminate the imbalance in the distribution of medical workers by levels and conditions of medical care. Based on these needs, a new labor behavior of medical workers is being formed, which depends on many factors and needs a high-quality and in-depth study, not only in theory, but also in practice. This article presents the results of studies of the factors affecting labor behavior in the gender aspect. Labor attitudes and values, attitude toward work, labor activity, and job satisfaction are analyzed. There are presented the tools for both quantitative and qualitative analysis of the raised issues and formation of personnel reserve. The purpose of the article is to identify the gender aspects of the labor behavior of doctors at healthcare institutions using the example of Moscow. On the basis of the conducted study were identified qualitative gender differences and developed tools that could be used in healthcare organizations for analysis of personnel’s work, internal staff rotation, formation of personnel reserve.