The Salang Road, a critical transportation corridor, serves as a vital link connecting the northern and southern parts of Afghanistan. In response to the government's decision to upgrade this road, assessing and documenting the plant species in the region is crucial. This study was conducted to catalog the plant species along the Salang Road, aiming to establish a comprehensive understanding of the area's botanical diversity. Plants were recorded using the quadrate sampling method at 35 sampling points. A transect line was set up at each sampling point, 5 quadrats were established along each line, and the occurrence of plants within the quadrat frame was recorded. Quadrat sizes, determined using the minimal area method, were 10×10 m for trees, 2×5 m for shrubs, and 1 m2 for herbs. Plants were identified with the help of literature and by comparing with the deposited specimen of Kabul University Faculty of Sciences Herbarium (KUFS). Data were analyzed using Microsoft Excel. 135 diverse species, including trees, shrubs, and herbs, representing various ecological niches were documented. These species belong to 46 plant families, and the most abundant ones were Asteraceae, Apiaceae, Rosaceae, Lamiaceae, Fabaceae, and Polygonaceae. The survey additionally examined road construction's impact on plant species' distribution and diversity. The survey provides valuable insights into the Salang corridor area's botanical diversity and ecological significance, serving as a foundational dataset for future environmental conservation and land management efforts. The information obtained from this study can support qualified decision-making and sustainable development practices in the region.