Qinghai-Tibet Plateau Of China Research Articles

Genomic Characterization and Comparative Analysis of Streptococcus zhangguiae sp. nov. Isolated from the Respiratory Tract of Marmota Himalayana.

Two Gram-stain-positive, oxidase-negative, non-motile, facultative anaerobic, α-hemolytic, coccus-shaped bacteria (zg-86T and zg-70) were isolated from the respiratory tracts of marmots (Marmota Himalayana) on the Qinghai-Tibet Plateau of China. Phylogenetic analysis of the 16S rRNA gene and 545 core genes revealed that these two strains belong to the Streptococcus genus. These strains were most closely related to Streptococcus respiraculi HTS25T, Streptococcus cuniculi CCUG 65085T, and Streptococcus marmotae HTS5T. The average nucleotide identity (ANI) and digital DNA‒DNA hybridization (dDDH) were below the threshold for species delineation. The predominant cellular fatty acids (CFAs) in this novel species were C16:0, C18:0, and C18:1ω9c, whereas the primary polar lipids were phosphatidylglycerol (PG), phosphatidylethanolamine (PE) and an unknown phosphoglycolipid (PGL). The optimal growth conditions for the strains were 37°C, pH 7.0, and 0.5% (w/v) NaCl on brain-heart infusion (BHI) agar supplemented with 5% defibrinated sheep blood. Comparative genomics analyses revealed the potential pathogenicity of strain zg-86T through comparisons with suis subclade strains in terms of virulence factors, pathogen-host interactions (PHIs) and mobile genetic factors (MGEs). Based on the phenotypic characteristics and phylogenetic analyses, we propose that these two isolates represent novel species in the genus Streptococcus, for which the names Streptococcus zhangguiae sp. nov. (the type strain zg-86T=GDMCC 1.1758T=JCM 34273T) is proposed.

The Response of Grassland Production to N and P Fertilizer Management Mediated soil Nematode Community in Qinghai-Tibet Plateau of China

The Response of Grassland Production to N and P Fertilizer Management Mediated soil Nematode Community in Qinghai-Tibet Plateau of China

Impact of temperatures on grassland transfer strategy: Evidence from the Qinghai-Tibet plateau in China

The increasing severity and complexity climate change present significant challenges to agricultural and social activities. This study investigates the effects of extreme temperatures on grassland transfer strategies, utilizing micro-level survey datasets obtained from pastoral areas of the Qinghai-Tibet Plateau in China, which are then matched with weather data. Employing the piecewise linear method and the temperature bins approach, the results reveal that extreme high temperature weather has a positive and significant effect on grassland transfer decisions. Specifically, each additional day of high-temperature weather throughout the year increases the overall probability of grassland transfer by 0.52%. Additionally, the analysis reveals a U-shaped relationship between temperature and grassland transfer-in decision, with the turning point occurring in the of 5–8 °C range. Furthermore, the findings indicate that grassland transfer decisions are predominantly influenced by moderating effects of factors such as the grassland quality and non-pastoral income. Moreover, herder households characterized by high income levels and younger demographics exhibit a notable capability in rapidly applying adaptive strategies to cope with temperature variations. Projections for future climate change scenarios indicate that extreme temperatures will significantly negatively impact grassland transfer-out, while positively influencing transfer-in.

Combined Multi-Omics Analysis Reveals the Potential Role of ACADS in Yak Intramuscular Fat Deposition.

The Yak (Bos grunniens) is a special breed of livestock predominantly distributed in the Qinghai-Tibet Plateau of China. Intramuscular fat (IMF) content in beef cattle is a vital indicator of meat quality. In this study, RNA-Seq and Protein-Seq were respectively employed to sequence the transcriptome and proteome of the longissimus dorsi (LD) tissue from 4-year-old yaks with significant differences in IMF content under the same fattening conditions. Five overlapping genes (MYL3, ACADS, L2HGDH, IGFN1, and ENSBGRG00000000-926) were screened using combined analysis. Functional verification tests demonstrated that the key gene ACADS inhibited yak intramuscular preadipocyte (YIMA) differentiation and proliferation, promoted mitochondrial biogenesis gene expression, and increased the mitochondrial membrane potential (MMP). Furthermore, co-transfection experiments further demonstrated that interfering with ACADS reversed the effect of PPARα agonists in promoting lipid differentiation. In conclusion, ACADS potentially inhibits lipid deposition in YIAMs by regulating the PPARα signalling pathway. These findings offer insights into the molecular mechanisms underlying yak meat quality.

Characterization of Co-bearing pyrite from the SEDEX Tuolugou Co(Au) deposit, northern Qinghai-Tibet Plateau, China

The Tuolugou sedimentary-exhalative (SEDEX) Co-Au deposit is located in the eastern Kunlun metallogenic belt of the northern Qinghai-Tibet Plateau in China. Pyrite is the prevalent Co-hosting sulfide mineral that occurs in the Tuolugou deposit. In this study, we investigated mineral characteristics of Co-bearing pyrite and effect of Co on crystal structure of the pyrite to obtain a better understanding of Co mineralization. Three pyrite generations were identified, including Co-barren pyrite (Py-1, formed during the early mineralization stage I, with Co content of 1-3844 ppm), Co-rich pyrite (Py-2, formed during the sedimentary-exhalative stage II, Co content 15–36522 ppm), and Co-rich pyrite (Py-3, formed during metamorphic stage III, with Co contents of 448–10505 ppm in Py-3A, and 1371–14904 ppm in Py-3B). As Co enters the pyrite structure, it is discovered that the octahedrons in pyrite is twisted and tilted, the lattice parameters (a0) rise, and the sulfur atom positions alter. The Co-rich Py-2 shows a similar REE and trace element distribution as those in quartz albitite, which indicates that Co mineralization is related to SEDEX genesis. Subsequent metamorphism resulted in additional Co enrichment. This detailed study of Co-bearing pyrite provides insights on the details of isomorphism Co in pyrite similar to the situation of this deposit as medium–low Co grade.

Coupled effects of UV radiation and freeze–thaw cycles on the fracture behavior of asphalt concrete

The Qinghai-Tibet Plateau in China is characterized by extreme climate conditions, including intense ultraviolet (UV) radiation, frequent freeze–thaw cycles (FTCs), and significant temperature fluctuations. These conditions contribute to severe cracking in asphalt pavements. This study aims to investigate the impact of coupled damage from UV radiation and FTCs on the fracture behavior of asphalt concrete. To simulate the coupled environmental damage (CED) experienced by asphalt pavement in the field, an alternating two-factor laboratory procedure was developed. This procedure reflects the climatic conditions of the Qinghai-Tibet Plateau. The semi-circular bending test, combined with digital image correlation technology, was employed to evaluate the effects of temperature and CED cycles on fracture-related parameters of asphalt concrete. These parameters include the load–displacement curve, fracture toughness, and fracture energy. Fracture behavioral characteristics such as horizontal strain, crack length, crack propagation path, and crack mouth opening displacement were also analyzed. The results indicate that coupled damage from UV radiation and FTCs significantly weakened the fracture resistance of asphalt concrete. Compared with other fracture-related indicators, the most significant reduction observed was the fracture energy of 68.1%. The two environmental factors exhibited a mutually reinforcing effect on the fracture properties of asphalt concrete, with low temperatures further intensifying their influence. CED cycles delayed the initiation of cracks in asphalt concrete but accelerated crack propagation. This led to a reduction in time to failure by up to 57.6% and a maximum reduction in horizontal strain of 24%. Additionally, the cracking propagation path of asphalt concrete became more linear with increased crack mouth opening displacement. Future research should focus on the evolution of damage accumulation in asphalt concrete under CED cycles, with the goal of establishing predictive models for long-term service life.

Diffusion characteristics of indoor pollutants from incense sticks combustion in Tibetan Plateau buildings: An experimental study

Incense burning, a common practice among ethnic Tibetans in Qinghai-Tibet Plateau of China, generates pollutants that can impact indoor air quality and, subsequently, human health. In this paper, we investigate the diffusion characteristics of four prevalent pollutants emitted during the burning of local Tibetan incense under low-pressure conditions specific to the plateau region. The diffusion behavior of these incense burning pollutants deviates from that observed under standard atmospheric pressure due to the unique conditions prevailing at high altitudes. The research results reveal that the primary pollutants released during Tibetan incense combustion are carbon dioxide, inhalable PM2.5, and PM10. Furthermore, ambient temperature exerts a substantial influence on the concentration of pollutants emitted during incense burning. A 6 °C increase in ambient temperature results in a 1.17-fold rise in the combustion rate. Consequently, higher temperatures correspond to larger diffusion coefficients, increased gas diffusion rates, and elevated pollutant concentrations at equivalent combustion times. Simultaneously, the low oxygen content characteristic of plateau regions has a notable impact on the diffusion rate of gaseous pollutants. This study of pollutant concentration and diffusion characteristics during incense burning offers valuable insights for determining the minimum ventilation rate in plateau areas.

Predicting potential and quality distribution of Anisodus tanguticus (Maxim.) Pascher under different climatic conditions in the Qinghai-Tibet plateau.

Anisodus tanguticus (Maxim.) Pascher, a distinctive medicinal plant native to the Qinghai-Tibet Plateau, China, has garnered attention due to increasing market demand. This study explores the impact of environmental factors on the distribution and levels of active compounds namely anisodamine, anisodine, and atropine within A. tanguticus. Our goal was to identify suitable cultivation areas for this plant. This study employs the maximum entropy model to simulate the suitable area of A. tanguticus under current conditions and three climate change scenarios during the 2050s and 2070s. The finding revealed that altitude, precipitation in the warmest season (Bio 18), the average annual temperature (Bio 1) exerted significant influences on the distribution of A. tanguticus. Among the environmental factors considered, temperature difference between day and night (Bio 2) had the most substantial impact on the distribution of anisodamine, temperature seasonal variation variance (Bio 4) predominantly influenced anisodine distribution, and Bio 1 had the greatest effected on the distribution of atropine. The suitable areas primarily exist in the eastern Qinghai-Tibet Plateau in China, encompassing a total area of 30.78 × 104 km2. Under the climate scenarios for the future, the suitable areas exhibit increasing trends of approximately 30.2%, 30.3%, and 39.8% by the 2050s, and 25.1%, 48.8%, and 60.1% by the 2070s. This research would provide theoretical suggestions for the protection, and cultivation management of A. tanguticus resources to face the challenge of global climate change.

Insights on droplet drift and effective utilization of pesticide in “Third Pole”：Qinghai-Tibet Plateau of China

Crop protection pesticide spraying aims to greatly improve the utilization rate of pesticides. Controlling pesticides deposition requires a thorough understanding of the spatial behaviour of spray droplets.The Qinghai-Tibet Plateau, which is the headwaters of three largest water resources (Yangtze, Yellow and Lancang) in China, has exceptionally unique climatic characteristics. The goal is to reduce the amount of pesticides entering water resources. The wind tunnel experiment was used to validate the discrete phase method for tracking the trajectories. Cooperation between the smaller and larger droplets (greater than 150 μm) in the dense area around the nozzle can undoubtedly enhance the initial dispersion of droplet sizes. Droplet coalescence, which lowers the proportion of readily dispersed droplets, can greatly boost droplet deposition onto the target location.The crucial drift height is presented and clarified when droplets gradually disperse by identifying the descending length at which efficiency of mass transit starts towards decrease off quickly. The pesticide transport efficiency will not be enhanced by reducing the initial relative spread of droplets if the actual spray height surpasses the crucial drift height, and may even worsen drift loss. The temperature and relative humidity of the air have a greater influence on the evaporation losses of droplets smaller than 150 μm. In addition to providing information about pesticide spraying, the results of studies on droplet drift behaviors also suggest a method for controlling drift.

Soil, Plant, and Microorganism Interactions Drive Secondary Succession in Alpine Grassland Restoration.

Plant secondary succession has been explored extensively in restoring degraded grasslands in semiarid or dry environments. However, the dynamics of soil microbial communities and their interactions with plant succession following restoration efforts remain understudied, particularly in alpine ecosystems. This study investigates the interplay between soil properties, plant communities, and microbial populations across a chronosequence of grassland restoration on the Qinghai-Tibet Plateau in China. We examined five succession stages representing artificial grasslands of varying recovery durations from 0 to 19. We characterized soil microbial compositions using high-throughput sequencing, enzymatic activity assessments, and biomass analyses. Our findings reveal distinct plant and microbial secondary succession patterns, marked by increased soil organic carbon, total phosphorus, and NH4+-N contents. Soil microbial biomass, enzymatic activities, and microbial community diversity increased as recovery time progressed, attributed to increased plant aboveground biomass, cover, and diversity. The observed patterns in biomass and diversity dynamics of plant, bacterial, and fungal communities suggest parallel plant and fungal succession occurrences. Indicators of bacterial and fungal communities, including biomass, enzymatic activities, and community composition, exhibited sensitivity to variations in plant biomass and diversity. Fungal succession, in particular, exhibited susceptibility to changes in the soil C: N ratio. Our results underscore the significant roles of plant biomass, cover, and diversity in shaping microbial community composition attributed to vegetation-induced alterations in soil nutrients and soil microclimates. This study contributes valuable insights into the intricate relationships driving secondary succession in alpine grassland restoration.

A Study on the Anti-inflammatory Activity of Clematis tangutica Using Molecular Networking

Background:: Clematis tangutica, an indigenous medicinal herb native to the Qinghai- Tibet Plateau of China, has traditionally been associated with treating various inflammation-related diseases. While its therapeutic potential is recognized, a comprehensive characterization of its metabolite molecules and their anti-inflammatory properties has not been undertaken. Objective:: This study aimed to comprehensively profile the metabolite molecules of Clematis tangutica and identify potential anti-inflammatory active molecules using the Activity Labelled Molecular Networking (ALMN) approach. Methods:: The ALMN approach was employed to visually label activity to the feature-based molecular network, allowing for the profiling of potential anti-inflammatory active molecules in Clematis tangutica. Through correlating activity levels with the respective molecules, a detailed profiling was achieved. Results:: Out of the 8,644 metabolite molecules in Clematis tangutica, ten were identified as the most potent anti-inflammatory molecules. Among these, Spiraeoside was notably annotated along with its structure. Conclusion:: This research successfully identified ten potent anti-inflammatory molecules from the vast metabolite profile of Clematis tangutica, including a detailed annotation of Spiraeoside. This marked a significant step in bridging traditional therapeutic knowledge with modern molecular profiling techniques.

Chromosome-level genome assembly of Hippophae gyantsensis

Hippophae gyantsensis, which is a native tree species in China, is ideal for windbreak and sand-fixing forests. It is an economically and ecologically valuable tree species distributed exclusively in the Qinghai-Tibet Plateau in China. In our study, we assembled a chromosome-level genome of H. gyantsensis using Illumina sequencing, Nanopore sequencing and chromosome structure capture technique. The genome was 716.32 Mb in size with scaffold N50 length of 64.84 Mb. A total of 716.25 Mb genome data was anchored and orientated onto 12 chromosomes with a mounting rate of up to 99.99%. Additionally, the genome was found to comprise approximately 56.84% repeat sequences, of which long terminal repeats(LTRs) that accounted for 33.19% of the entire genome. Meanwhile, a total of 32,316 protein-coding genes were predicted, and 91.07% of these genes were functionally annotated. We also completed a series of comparative genomic analyses to provide researchers with useful reference material for future studies on seabuckthorn.

Multi-isotopic composition (Li and B isotopes) and hydrochemistry characterization of the Lakko Co Li-rich salt lake in Tibet, China: Origin and hydrological processes

A comprehensive comprehension of the origin and ore-forming process of lithium brine deposits is imperative prior to engaging in lithium resource exploration, development and utilization. Nevertheless, the provenance of lithium in salt lakes has always been controversial. The lithium and boron (Li and B) isotopes, which serve as discerning geochemical tracers, can facilitate the monitoring of geological processes and sources. In this study, we investigated the hydrochemistry, Li and B isotope characteristics of surface brine and recharge river samples collected from the Lakko Co on the Qinghai-Tibet Plateau in China. The dissolved Li concentrations range from 0.11 to 590.8 mg/L, δ7Li and δ11B values exhibit a range of 0.75∼8.11 ‰ and -18.29∼-4.01 ‰, respectively. Concentrations of elements such as Rb, and Cu are relatively high and exhibit a strong correlation with Li. Ions composition and content ratios indicate the deep origin of Li and B. The analysis of δ7Li and δ11B suggests that, apart from hot spring, weathering of surrounding rocks also contributes to the presence of Li and B. Besides, variations in δ11B values within the lake brine analysis may be attributed to the deposition of water Magnesite at the lake bottom. The findings suggest that the recharge water system and hot springs serve as the primary source of Li and B in Lakko Co. Additionally, the interaction between water and rock in the deep crust as well as chemical weathering in the shallow environment, significantly contribute to formation of Li-rich fluids. The transportation process is characterized by the precipitation of secondary mineral, resulting in an increase of δ7Li and δ11B. Furthermore, the presence of water Magnesite in the lake brine leads to fluctuations in δ11B. This investigation of the lake contributes to a more comprehensive understanding of the origins and mechanisms involved in the transportation and storage of lithium in lithium-rich salt lakes.

Pasteurella multocida strains of a novel capsular serotype and lethal to Marmota himalayana on Qinghai-Tibet plateau in China

Pasteurella multocida is a zoonotic pathogen causing serious diseases in humans and animals. Here, we report P. multocida from wildlife on China's Qinghai-Tibet plateau with a novel capsular serotype, forming a single branch on the core-genome phylogenetic tree: four strains isolated from dead Himalayan marmot (Marmota himalayana) and one genome assembled from metagenomic sequencing of a dead Woolly hare (Lepus oiostolus). Four of the strains were identified as subspecies multocida and one was septica. The mouse model showed that the challenge strain killed mice within 24 h at an infectious dose of less than 300 bacteria. The short disease course is comparable to septicemic plague: the host has died before more severe pathological changes could take place. Though pathological changes were relatively mild, cytokine storm was obvious with a significant rise of IL-12p70, IL-6, TNF-αand IL-10 (P < 0.05). Our findings suggested P. multocida is a lethal pathogen for wildlife on Qinghai-Tibet plateau, in addition to Yersinia pestis. Individuals residing within the M. himalayana plague focus are at risk for P. multocida infection, and public health warnings are necessitated.

Research on Ecological Restoration Evaluation Index System for Construction Projects in Qinghai Tibet Plateau

In the “Ecological Protection Law of the People’s Republic of China on the Qinghai Tibet Plateau” released in April 2023, it is stipulated that “the state strengthens the ecological protection and restoration of the Qinghai Tibet Plateau, adheres to the integrated protection and restoration of mountains, rivers, forests, fields, lakes, grasses, sands, and ice, and implements a systematic governance that focuses on natural restoration and combines natural restoration with artificial restoration.” Currently, there is relatively little research on the ecological restoration evaluation index system in high-altitude areas represented by the Qinghai Tibet Plateau in China. This article studies the climate and soil characteristics of the Qinghai Tibet Plateau region and selects ecological restoration evaluation index systems suitable for high-altitude areas, including biological and community evaluation, soil and water conservation function evaluation, soil and water physical and chemical property evaluation, and landscape pattern evaluation.

Tibetan medicine Bang Jian: a comprehensive review on botanical characterization, traditional use, phytochemistry, and pharmacology.

Tibetan medicine Bang Jian refers to a range of botanical drugs within the Gentiana genus. It serves as a prominent traditional Tibetan botanical drug primarily found in the ethnic minority regions of the Qinghai-Tibet Plateau in China. Traditionally, the dried flowers of Bang Jian, known as "Longdanhua" have been employed in Tibetan medicine to address detoxification, pharyngeal relief, acute and chronic bronchitis, bronchiectasis, lung infections, pulmonary fibrosis, and throat disorders. Surprisingly, there has been no comprehensive review published to date on Tibetan medicine Bang Jian. This passage systematically presents and critically assesses recent advancements in botanical characterization, traditional applications, phytochemistry, pharmacology, and clinical uses of Bang Jian, aiming to provide a scientific foundation for its reasonable use and further exploration. To date, researchers have isolated and identified 92 structurally diverse compounds, with a predominant presence of iridoids, flavonoids, xanthones, and triterpenoids. The crude extracts and metabolites derived from Bang Jian have been found to exhibit a wide range of pharmacological effects, encompassing anti-inflammatory, anti-tumor, anti-bacterial, antiviral, antioxidant, hepatoprotective properties, and protect the respiratory system. Nevertheless, detailed data on the biological effects, metabolic activities, and mechanistic research concerning active monomer metabolites remain insufficient. Consequently, there is a pressing need for comprehensive and in-depth research to guide rational clinical drug usage and evaluate the medicinal attributes of Bang Jian.

Common pool resource governance in strong-government context: A case study of caterpillar fungus (Ophiocordyceps sinensis) on the Qinghai-Tibet Plateau

The common pool resource (CPR) theory has inspired the recognition of community self-governance in natural resource management. However, many CPR literature treated government as an external variable to community, without sufficiently considering the close interaction between the two, especially in strong-government context. In this paper, we applied two case villages on the Qinghai-Tibet Plateau of China with different governance systems for caterpillar fungus (Ophiocordyceps sinensis), a CPR with lucrative values, to study the effects of the different government interventions on the local social conditions and rangeland ecosystem. One village interacted with facilitative local government that helped to develop a pro-community self-governance institution, while the other faced a forceful government that substituted the community self-governance. Furthermore, we explored the underlying mechanism from the view of the congruence between the government actions to achieve administrative objectives and the community demands for self-governance. We found that the better outcomes occurred in the village with the facilitative government involvement, because the government actions were congruent with the community demands. The model with facilitative government involvement presents a possible approach, when CPR appropriator boundary became semi-opened to outsiders, by which the community self-governance institution could function well with government help in institution design, monitoring and sanction. Our findings enrich the applications of CPR governance theory, particularly in the aspect that how government-community interacts to develop a self-governance institution in strong-government context.

Molecular Cloning and Functional Characterization of the 5′ Regulatory Region of the SLC11A1 Gene from Yaks

The solute transport protein family 11 A1 (SLC11A1), also recognized as natural resistance-associated macrophage protein 1 (NRAMP1), represents a transmembrane protein encoded by the SLC11A1 gene. A variety of prior investigations have illuminated its involvement in conferring resistance or susceptibility to bacterial agents, positioning it as a promising candidate gene for breeding disease-resistant animals. Yaks (Bos grunniens), renowned inhabitants of the Qinghai-Tibet Plateau in China, stand as robust ruminants distinguished by their adaptability and formidable disease resistance. Notwithstanding these unique traits, there is scant literature on the SLC11A1 gene in the yak population. Our inquiry commences with the cloning of the 5′ regulatory region sequence of the Zhongdian yak SLC11A1 gene. We employ bioinformatics tools to identify transcription factor binding sites, delineating pivotal elements like enhancers and cis-acting elements. To ascertain the promoter activity of this region, we amplify four distinct promoter fragments within the 5′ regulatory region of the yak SLC11A1 gene. Subsequently, we design a luciferase reporter gene vector containing four site-specific deletion mutations and perform transient transfection experiments. Through these experiments, we measure and compare the activity of disparate gene fragments located within the 5′ regulatory region, revealing regions bearing promoter functionality and discerning key regulatory elements. Our findings validate the promoter functionality of the 5′ regulatory region, offering preliminary insights into the core and principal regulatory segments of this promoter. Notably, we identified single nucleotide polymorphisms (SNPs) that may be associated with important regulatory elements such as NF-1 and NF-1/L. This study provides a theoretical framework for in-depth research on the function and expression regulation mechanism of the yak SLC11A1 gene.

Decoding the DNA of scat and the application of genetic methodologies to understanding carnivore diet

Carnivore species are vital to ecosystem function and maintenance. One key component to understanding carnivore ecology and the most effective means of management is knowledge of dietary resource use. Traditional methods used to study carnivore diet, such as microhistology, have several technical and logistical shortcomings. These have hindered the quality and quantity of data that shape understanding of how carnivores exploit prey. Advances in genetic methodologies and their application to wildlife biology has transformed the manner in which information about species can be gained. DNA metabarcoding is one such example. With this approach, genetic sequences present in scat can be determined via next-generation sequencing and matched to reference databases, revealing the carnivore that deposited the scat and the prey it consumed. DNA metabarcoding has the ability to overcome many of the previous challenges associated with dietary analysis and works to advance and inform current knowledge surrounding carnivore ecology, predatorprey relationships, conflicts between carnivores and humans, and potential adaptability to large-scale landscape shifts. Its use has provided novel insights on numerous carnivore species to help inform research priorities and wildlife policies, including those living in unique fragile environments such as the Qinghai-Tibetan Plateau of China. The continued development and increased capacity of molecular dietary analysis via DNA metabarcoding has the promise to grossly improve carnivore conservation management strategies on a global scale.

An integrative methodology framework for assessing regional ecological risk by land degradation using the case of the Qinghai–Tibet Plateau

Land degradation poses significant threats to the sustainability of ecosystem structures, functions, and services, leading to increasing ecological risks. However, integrative assessment of the ecological risk driven by land degradation remains a challenge. In this study, we established a methodological framework for assessing regional ecological risk by integrating degenerative land use transitions with multiple ecological indicators. Specifically, 11 degradation modes of land use transitions and 7 indicators on ecosystem structures, functions, and services are quantitatively integrated using remote sensing data from 2000 to 2020 in the Qinghai-Tibet Plateau of China. Results revealed that the comprehensive ecological risk of the plateau is higher in the southern and northern regions. Furthermore, we found that land development from forests towards cropland and degradation towards grasslands lead to higher ecological risks than grassland development and degradation. The ecological risk tends to show a significant distance decay pattern around the patches with land degradation. Our research framework provides an efficient, explicit, and transferable means of exploring spatiotemporal changes in ecological risk caused by degenerative land use transitions at the regional scale. It presents a constructive tool for facilitating regional-scale land use and ecosystem management planning and assessment.