Qingdao Coast Research Articles

All-Weather Monitoring of Ulva prolifera in the Yellow Sea Based on Sentinel-1, Sentinel-3, and NPP Satellite Data

Ulva prolifera (U. prolifera), a global eco-environmental issue, has been recurring annually in the Yellow Sea of China since 2007, leading to significant impacts on the coastal ecosystem and the economies of coastal cities. To enhance the frequency of daily monitoring for U. prolifera and to advance the multi-source remote sensing monitoring system, a combination of the Sentinel-1 SAR remote sensing satellite and the Sentinel-3 OLCI and NPP VIIRS optical remote sensing satellites was employed. This comprehensive analysis encompassed the examination of Sentinel-1 C band characteristics, the range of influence of U. prolifera, and the migration trajectory of its enrichment zones. On 6 June 2021, three satellite images depicted the northwest drift of U. prolifera, followed by a southward movement after making contact with the coast of Qingdao, China, on 12 June. The most extensive impact area caused by U. prolifera was observed on 18 June. Subsequently, the images revealed a contraction and enrichment of U. prolifera in an eas–-west direction. The amalgamation of radar and optical remote sensing satellites in a multi-frequency monitoring approach allows for a continuous all-weather surveillance mechanism for U. prolifera. This mechanism serves to provide timely alerts for the prevention and management of U. prolifera outbreaks.

Occurrence, dietary influence and risks of selected trace metals in different coastal predatory species

The global issue of ongoing trace metal emissions and legacy accumulation from diverse sources is posing threats to coastal wildlife. This study characterized the distribution of five metals in relation to dietary ecology (carbon and nitrogen stable isotopes: δ15N and δ13C) in representative predatory species (starfish, fish, and seabird) collected from the coast of Qingdao, northeastern China. Zinc (Zn) was the most abundant metal across species, followed by copper (Cu), chromium (Cr), cadmium (Cd), total and methylated mercury (THg and MeHg). Among the studied species, black-tailed gulls (Larus crassirostris) occupied the highest trophic position, followed by three predatory fish species, whereas the northern Pacific seastar (Asterias amurensis) had the lowest trophic position. The starfish exhibited high capacity to accumulate Cd, Cr and Cu. Conversely, black-tailed gulls exhibited high levels of Zn, while Hg was highest in predatory fishes. Across species, Cr, MeHg, THg and MeHg:THg showed significant positive correlations with δ13C, suggesting the influence of inshore food sources on their accumulation. Both MeHg and THg were significantly and positively correlated with δ15N, with MeHg demonstrating a greater slope, indicating their potential trophic magnification. We assessed health risks from the studied metals using established toxicity reference thresholds. Elevated risks of Hg were identified in three predatory fish species, while other metals and species remain within safe limits. These findings emphasize the significance of foraging patterns in influencing trace metal accumulation in coastal predators and highlight the importance of further monitoring.

Photobacterium obscurum sp. nov., a marine bacterium isolated from the coast of Qingdao.

A Gram-stain-negative, facultative anaerobic, rod-shaped strain, named SDRW27T, was isolated from offshore seawater collected near Qingdao. Strain SDRW27T was able to grow at 16-37 °C (optimum, 28 °C), pH 6.0-9.0 (optimum, pH 6.0) and in the presence of 1-7 % (w/v) NaCl (optimum, 3 %). Phylogenetic analysis using 16S rRNA gene sequences indicated that strain SDRW27T was most closely related to Photobacterium toruni H01100410BT (97.89 % sequence similarity), Photobacterium andalusiense H01100409BT (97.89 %) and Photobacterium leiognathi ATCC 25521T (97.82 %). The predominant fatty acids were summed feature 3 (C16 : 1 ω7c and/or iso-C15 : 0 2-OH), summed feature 8 (C18 : 1 ω7c and/or C18 : 1 ω6c) and C16 : 0. The polar lipids of strain SDRW27T comprised phosphatidylglycerol, phosphatidylinositol dimannoside, phosphatidylcholine, phosphatidylethanolamine and three unidentified lipids. The major respiratory quinone was ubiquinone-8. The G+C content was 47.71 mol%. The genome size was 5.84 Mbp, including 85 contigs with an N50 value of 223 542. The average nucleotide identity (ANI) values of SDRW27T with its three most similar strains, P. toruni H01100410BT, P. andalusiense H01100409BT and P. leiognathi ATCC 25521T, were 71.36, 71.58 and 72.23 %, respectively (all lower than the 95-96 % ANI threshold), and the DNA-DNA hybridization (DDH) values were 20.4, 20.8 and 20.4 % (all lower than the 70 % DDH threshold). The obtained results of polyphasic analysis demonstrate that strain SDRW27T represents a novel species, for which the name Photobacterium obscurum sp. nov. is proposed. The type strain is SDRW27T (=MCCC 1K06286T=KCTC 82892T).

Spartinivicinus marinus sp. nov., isolated from intertidal sediment.

A Gram-stain-negative, aerobic, flagellated, and long rod-shaped bacterium, designated strain SM1973T, was isolated from an intertidal sediment sample collected from the coast of Qingdao, PR China. Strain SM1973T grew at 15-37 °C and with 0-5.5 % NaCl. It reduced nitrate to nitrite and hydrolysed aesculin but did not hydrolyse casein and gelatin. The strain showed the highest 16S rRNA gene sequence similarity (98.2 %) to the type strain of Spartinivicinus ruber. The phylogenetic trees based on the 16S rRNA genes and single-copy orthologous clusters showed that strain SM1973T clustered with S. ruber, forming a separate lineage within the family Zooshikellaceae. The major cellular fatty acids were summed feature 3 (C16 : 1 ω7с and/or C16 : 1 ω6с) and C16 : 0. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The main respiratory quinone was ubiquinone-9. The genomic DNA G+C content of strain SM1973T was 40.4 mol%. Based on the polyphasic evidence presented in this paper, strain SM1973T is considered to represent a novel species within the genus Spartinivicinus, for which the name Spartinivicinus marinus sp. nov. is proposed. The type strain is SM1973T (=MCCC 1K04833T=KCTC 72846T).

Experimental investigation on the wave-oscillating buoy interaction and wave run-up on the buoy

The interaction between a wave and an oscillating buoy is complex, and it directly affects the stability, working state, and efficiency of the buoy. In this study, an experimental investigation of the interaction between a wave and an oscillating buoy (including wave breaking, wave run-up, and the relative motion between waves and buoys) is conducted under constant power take-off (PTO) values. The wave conditions selected in the experiment are all obtained from measured data on the Qingdao coast, China. The experimental results show that when the wave steepness is greater than 0.0597, there is an obvious wave breaking in front of the oscillating buoy and that the steepness of the critical wave decreases with an increase in PTO. When the relative damping force is 0.101. In addition, an empirical formula based on the wave steepness, the PTO value, and the wave run-up is proposed in accordance with the M5’ model tree. The results obtained in this study contribute to an increased understanding of the development of wave energy from buoys. In turn, this improvement in understanding will inform the design and deployment of buoys in real-life scenarios.

Robust ocean zoning for conservation, fishery and marine renewable energy with co-location strategy

With the increasing human-dominated seascape, maritime exploitation like fishery and marine renewable energy (MRE) is soaring in both abundance and intensity, which brings forward the ocean zoning problem to balance conservation, fishery, and MRE. What complicates the ocean zoning problem is the ambiguity of values (conservation, fishery, and MRE) and opportunity cost due to the scarce data and the complexity of the eco-economy system. To address the ocean zoning problem with ambiguity, we characterize these ambiguous values/cost as ambiguity sets containing all possible distributions from the robust optimization perspective and formulate the values of conservation, fishery, and MRE as three objectives in the setting of risk-averse decision-makers. Furthermore, we adopt the co-location strategy of fishery and MRE as constraints and assign a higher priority to the conservation objective. After deriving and solving an ambiguous multi-objective risk-averse ocean zoning model, we perform the model analysis of the conflict, ambiguity, and Pareto optimality. A case study of the Huanghai sea under the jurisdiction of the West Coast of Qingdao justifies several significant insights. First, the MRE is fully willing to co-locate with the fishery under the pressure of economy and ocean use intensity. Second, the robust strategies incorporating the decision-dependent expectation and lower covariance can ensure the objectivity and rationality of ocean zoning for conservation, fishery, and MRE. Third, the risk-averse degree from ambiguous value plays a pivotal role in the fulfillment of aspiration levels and the zoning strategies. Overall, our method can improve the space allocation performance and provide a coordinated-development strategy for conservation and development.

Molecular Response of Ulva prolifera to Short-Term High Light Stress Revealed by a Multi-Omics Approach.

The main algal species of Ulva prolifera green tide in the coastal areas of China are four species, but after reaching the coast of Qingdao, U. prolifera becomes the dominant species, where the light intensity is one of the most important influencing factors. In order to explore the effects of short-term high light stress on the internal molecular level of cells and its coping mechanism, the transcriptome, proteome, metabolome, and lipid data of U. prolifera were collected. The algae were cultivated in high light environment conditions (400 μmol·m-2·s-1) for 12 h and measured, and the data with greater relative difference (p < 0.05) were selected, then analyzed with the KEGG pathway. The results showed that the high light stress inhibited the assimilation of U. prolifera, destroyed the cell structure, and arrested its growth and development. Cells entered the emergency defense state, the TCA cycle was weakened, and the energy consumption processes such as DNA activation, RNA transcription, protein synthesis and degradation, and lipid alienation were inhibited. A gradual increase in the proportion of the C4 pathway was recorded. This study showed that U. prolifera can reduce the reactive oxygen species produced by high light stress, inhibit respiration, and reduce the generation of NADPH. At the same time, the C3 pathway began to change to the C4 pathway which consumed more energy. Moreover, this research provides the basis for the study of algae coping with high light stress.

Salinimicrobium sediminilitoris sp. nov., Isolated from a Tidal Flat.

A Gram-negative, facultatively anaerobic, motile, and rod-shaped bacterium, designated ASW11-47T, was isolated from a tidal flat sediment taken from the coast of Qingdao, PR China. Phylogenetic analysis of 16S rRNA gene sequence showed that strain ASW11-47T belongs to the genus Salinimicrobium and is most closely related to Salinimicrobium terrae YIM-C338T (98.68% similarity). The length of draft genome is 3,594,457bp, and DNA G + C content is 40.8mol%. The values of average nucleotide identity and digital DNA-DNA hybridization between strain ASW11-47T and closely related strains were in ranges of 75.9-85.9 and 19.7-31.5%, respectively. The major fatty acids (> 10%) were iso-C15:0 and iso-C17:0 3-OH. The predominant respiratory quinone was menaquinone-6 and the major polar lipid was phosphatidylethanolamine. On the basis of genotypic, phenotypic, and chemotaxonomic analysis, strain ASW11-47T represents a novel species within the genus Salinimicrobium, for which the name Salinimicrobium sediminilitoris sp. nov. is proposed. The type strain is ASW11-47T (= KCTC 82501T = MCCC 1K05586T).

Development and trials of a novel deep-sea multi-joint autonomous underwater vehicle

To realize fine exploration in the deep sea, this paper designed a highly maneuverable multi-joint autonomous underwater vehicle (MJ-AUV). MJ-AUV is composed of three cabins in series, which are connected mechanically and electrically through two joint systems. The rotation of the joints drives the deformation of MJ-AUV, thereby realizing a variety of maneuvering modes. Each connecting joint is driven by a two-stage bevel gear, which is compact in structure and has good strength and rigidity, and can drive the cabins to achieve two degrees of freedom (i.e., pitch and yaw) motion. Computational fluid dynamics (CFD) technology was used to build a numerical pool and calculate the added mass coefficient and drag coefficient of each cabin. The kinematics model of MJ-AUV was established and the force analysis was carried out. On this basis, the simulation environment was built through computational multi-body dynamics (MBD) software MSC Adams, which was used to systematically analyze the multiple motion states of MJ-AUV. Lake and sea trials conducted in Thousand Islet Lake, the South China Sea, Qingdao Coast, and other places have validated the actual performance of the designed MJ-AUV, including cruising mode, vertical profile motion, deep diving and water surface motion.

Distribution of Dinoflagellate Cysts in Surface Sediments From the Qingdao Coast, the Yellow Sea, China: The Potential Risk of Harmful Algal Blooms

Surface sediments were collected from three sea areas of the Qingdao coast, the Yellow Sea, China, namely, the inner Jiaozhou Bay, the Laoshan coast, and the Amphioxus Reserve area in November to December 2017. Dinoflagellate cysts were observed in the sediments, focusing on the distribution of toxic and harmful species. Contents of biogenic elements were analyzed to reveal their relationships to cysts. A total of 32 cyst taxa were identified, including 23 autotrophic and 9 heterotrophic taxa. Cyst concentrations ranged from 83.3 to 346.5 cysts/g D Wt with an average of 210.7 cysts/g D Wt. Generally, cysts of autotrophic dinoflagellates dominated in sediments from the Qingdao coast with proportions of 41.05%–90.25%. There were no dominant group in cyst assemblages; cysts of Protoperidiniaceae, Suessiales, and Calciodinelloideae showed similar contributions. Cyst assemblages were quite different in the inner Jiaozhou Bay reflected by the lower species richness, diversity, and cyst concentration. Results from the redundancy analysis (RDA) demonstrated the influence of biogenic elements on cyst assemblages, which explained well why the three sea areas with different degrees of human activities showed different dinocyst storages. Notably, 17 harmful algal bloom (HAB) dinoflagellate cysts were identified in this study, including cysts of those producing toxins that may damage human health and marine animals. Some of these cysts occurred widely and dominantly in this study, such as cysts of Gonyaulax spinifera, Azadinium trinitatum, Scrippsiella acuminata, and Biecheleria halophila, suggesting the potential risk of HABs in the Qingdao coastal area.

Investigating Spatial Distribution of Green-Tide in the Yellow Sea in 2021 Using Combined Optical and SAR Images

Optical remote sensing is limited to clouds and rain. It is difficult to obtain ground object images in severe weather. Microwave remote sensing can penetrate clouds and rain to obtain ground object images. Therefore, this paper combines optical and microwave data to analyze the time and space of the green-tide in the Yellow Sea in 2021. Compared with a single data source, the distribution characteristics increase the frequency of time observation and show the green-tide changes in more detail. The continuous remote sensing observation time is 80 days. Ulva prolifera has experienced discovery (mid-late May), development (mid-late May to early June), outbreak (early June to mid-late June), decline (late June to mid-July), and extinction (late July to mid-August) in five stages; the development period drifts along the northeast direction, the outbreak period drifts along the northwest direction, the decline and extinction periods are mainly in the Rizhao and Qingdao waters. Ulva prolifera has a tendency to drift northward as a whole, drifting through Yancheng, Lianyungang, Linyi, Rizhao and Qingdao waters eventually landing on the coast of Qingdao and gradually disappearing.

Molecular phylogeny and taxonomy of four Remanella species (Protozoa, Ciliophora): A flagship genus of karyorelictean ciliates, with descriptions of two new species.

During faunal studies of psammophilic ciliates along the coast of Qingdao, China, several marine karyorelictean species were isolated. Among them, four species within the genus Remanella were investigated, including two species new to science: i.e., R. rugosa, Remanella elongata sp. nov., Remanella aposinica sp. nov., and R. unicorpusculata. Remanella rugosa has been reported several times, but this study is the first to provide detailed morphological characters and phylogenetics. Remanella elongata sp. nov. can be distinguished from its congeners by the presence of complex cortical granules, fewer macronuclei, and longer body size. Remanella aposinica sp. nov. differs from its congeners by having 14–17 right lateral ciliary rows and 24–37 dikinetids of intrabuccal kinety. Poorly known Remanella rugosa var. unicorpusculata (Kahl, 1933) Foissner, 1996 should be elevated from subspecies to species level, Remanella unicorpusculata (Foissner, 1996) stat. nov., based on detailed redescriptions with statistical data, living morphology, infraciliature, and species definitions. Small subunit (SSU) rDNA was sequenced for the four species, and phylogenetic analysis revealed that all known taxa in Remanella formed the outline branch to the genus Loxodes with moderate to high bootstrap support among Remanella lineages.

Ulva macroalgae within local aquaculture ponds along the estuary of Dagu River, Jiaozhou Bay, Qingdao

Green macroalgal blooms caused by Ulva species have influenced the Shandong Province for 15 consecutive years since 2007, leading to serious damage to the marine environment. Great biomasses of attached Ulva prolifera on Neopyropia aquaculture rafts in the Yellow Sea were considered as sources of blooms. However, it is still unclear whether U. prolifera could survive and settle in the Qingdao coastal environment, Shandong, thus leading to local Ulva blooms in the future. In this research, sampling of Ulva macroalgae in seven Portunus trituberculatus aquaculture ponds along the coast of Qingdao was conducted on August 21, 2019. In total, 24 samples collected from the ponds were analyzed through methods of molecular biological identification (ITS, 5S, and rps2-trnL sequences) and genetic analysis. All the aquaculture ponds contained large amounts of floating Ulva macroalgae, which consisted of three species: U. prolifera, Ulva meridionalis, and Ulva pertusa. Among these species, U. meridionalis, which is usually found in southern Japan, also causes the green tide. In addition, all Ulva macroalgae floated on the surfaces of aquaculture ponds, and were discarded into the local coastal area by aquaculturist. This research raised our awareness of the importance of controlling the spread of the green tide related macroalgae.

Comparison in diversity of eukaryotic algae in surface sediments from different functional sea areas of Qingdao coast, the Yellow Sea, China: a metabarcoding approach

Comparison in diversity of eukaryotic algae in surface sediments from different functional sea areas of Qingdao coast, the Yellow Sea, China: a metabarcoding approach

Comparing performance of inter-sensor NDVI for the detection of floating macroalgal blooms in the Yellow Sea

There are many complains against macroalgal bloom (MAB) from all over the world for its high negative economic impacts. Early and precise detection of MAB occurance can reduce huge economic loss in tourism and marine business. Thus, this study aims to detect the MAB in the Yellow Sea (YS) by comparing performance of Normalized Difference Vegetation Index (NDVI) between Geostationary Ocean Color Imager (GOCI) and Landsat-8 sensors from 2014 to 2019. The largest MAB during this period was identified from Landsat-8 sensors along the coast of Sheyang and Qingdao of China in 2018. The locations of MABs were almost nearby areas during the begining phase of MABs in consecutive years. In successive years huge and dense MABs reached the brink of shoreline of Qingdao. In Landsat-8 images in most cases larger total areas of MABs were detected including very small pathches which could not be detected from GOCI due to low spatial resolution. The findings of this study will be helpful for scientists to choose between GOCI and Landsat-8 based on the objectives of their study.

Utilization and release of biogenic elements by macroalgae Ulva prolifera: A mesocosm experiment off the coast of Qingdao, China

A mesocosm experiment was conducted on the Qingdao coast to study the effects of nutrient enrichment on the physiological responses of Ulva prolifera and the changes in the entire culture system during decomposition of algal blooms. The decomposition trend of U. prolifera off the Qingdao coast was slightly retarded by nutrient enrichment, and could not be reversed to positive growth. The decomposition of U. prolifera was divided into two stages: the early-stage (0–9 d) and the late-stage (9–19 d). High nutrient levels enhanced the amounts of carbon and nitrogen fixed by U. prolifera and accelerated the subsequent degradation of organic matter. The concentration and composition of amino acids in solution also changed, reducing the bioavailability of dissolved organic matter in seawater. This study evaluated the actual decomposition process of green tides, which will help to assess the impact of green tides on coastal ecosystems and the circulation of biogenic elements.

The seasonal distribution characteristics of microplastics on bathing beaches along the coast of Qingdao, China

Microplastics pollution in nearshore marine environment has become increasingly prominent and has received widespread attention. As a major leisure and entertainment place in coastal cities, bathing beaches not only provide an environment for microplastics to enter the ocean, but also provide a place for the retention of microplastics. In this paper, the distribution, composition and seasonal variation of microplastics in seawater and sediments of six bathing beaches along the coast of Qingdao were studied. The results showed that the average abundance of microplastics in the sediments of the Qingdao bathing beach was 91.11 ± 26.76 items/m2 (13.77 ± 4.39 items/kg) in the winter and 147.78 ± 34.80 items/m2 (21.98 ± 5.97 items/kg) in the summer. The microplastic abundance in the seawater in the summer (average: 567.50 ± 101.06 items/m3) was significantly higher than that in the winter (average: 326.11 ± 49.03 items/m3, p < 0.05). The abundance of microplastics at the six bathing beaches increased from east to west, consistent with the transport direction of coastal currents. The microplastic abundance in the sediment of non-swimming zone of the bathing beach was significantly higher than that in swimming zone (p < 0.05), and the microplastic abundance in the seawater of the swimming zone was slightly higher than that of the non-swimming zone but with no significant difference (p > 0.05). To an extent, it can reflect the correlation between the abundance of microplastics and human recreational activities. The microplastics detected at the bathing beaches were mainly lines (80.5%) and fragments (7.9%) with a size range of 100 to 1000 μm. Rayon (41.8%) and polyethylene terephthalate (PET, 16.9%) were the main polymer types. The microplastic characteristics showed significant seasonal differences, more diversity in summer than in winter. These results emphasized that the distribution and movement of microplastics in bathing beaches were affected by seasons, human activities and coastal currents.

A Spatiotemporal Change Detection Analysis of Coastline Data in Qingdao, East China

This study focuses on the coastal features, environments, and dynamics to accurately describe and regularly monitor the Qingdao shoreline in eastern China. It collects categorical ETM+ and OLI data from 2000, 2010, and 2019 on the mainland coastline and explores the characteristics and spatiotemporal differences across the past 19 years by using remote sensing and geographic information system (GIS) technologies. The results show that the length of the Qingdao coastline has increased continuously over the last two decades, for a total increase of 18.14 km. There are different natural and artificial coastlines that have undergone major changes. The human-induced deterioration of coastlines has gradually and substantially risen from 53.63% in 2000 to 68.40% in 2019, while the length of the natural coastlines has decreased dramatically. Jiaozhou Bay focuses on areas with significantly changing coastlines, and major changes have occurred in the west and east of the Qingdao coast. The coastline has largely expanded seaward because of the comprehensive impact of natural and anthropogenic factors. The leading factor in coastal evolution is coastal engineering constructions. In addition, the top three other construction activities are the restoration of the aquaculture pond, salt field, and harbor edifices. The driving force that triggered the shift in the coastline reveals significant temporal heterogeneity.

Marine biomass-derived composite aerogels for efficient and durable solar-driven interfacial evaporation and desalination

• Construction of an interfacial steam generator by using nanocellulose extracted from marine biomass. • Rational fabrication of nanocellulose-based aerogels as structural elements. • Realization of a 3D monolithic interfacial steam generator for efficient and durable solar evaporation. Solar-driven interfacial evaporation is an emerging and sustainable technology with growing potential for applications in water distillation and desalination. Despite the ongoing progress in clean water production, the high cost, delicate structures, leaching and disposal of synthetic materials remain the major roadblocks toward large-scale and real-world applications. Herein, nanocellulose (NC) is successfully extracted from abundant, inexhaustible and biodegradable biomass Ulva (Enteromorpha) prolifera that collected from the coast of Qingdao, China. Incorporation of polyvinyl alcohol (PVA) into the NC scaffolds and subsequent cross-linking endow the composite aerogels with efficient water diffusion, enhanced mechanical strength and good deformation resistance. The cross-linked composite aerogels can serve as main structural elements and integrate a monolithic, self-floating and durable steam generator. Under one sun, the good water evaporation rate of 1.4 kg m −2 h −1 is among the best-performing interfacial steam generators constructed by using cellulose-based materials as structural components. This study demonstrates a new concept of using marine (blue) biomass-derived NC as crude material and building block to construct high-performance and durable interfacial steam generators, synergistically considering clean water production and sustainability of marine ecosystems.

Emissions of biogenic sulfur compounds and their regulation by nutrients during an Ulva prolifera bloom in the Yellow Sea

Three cruises were conducted during an Ulva prolifera bloom in 2018 off the Qingdao coast to observe early-, late-, and after-bloom characteristics. Spatio-temporal changes in dimethylsulfide (DMS), dimethylsulphoniopropionate (DMSP), and acrylic acid (AA) and their relationships with environmental parameters were examined. Mesocosm experiments tested the effect of nutrient on the release of biogenic sulfur compounds during the decline period. Biogenic sulfur concentrations contrasted with those in non-bloom regions and reached their highest values during the late-bloom period. The average sea-to-air DMS flux was about 5 times higher than those of non-bloom regions. The mean concentrations of DMS and DMSP in the enclosures were 3–5 times those of in-situ seawater. Biogenic sulfur release was promoted by the addition of high concentrations of nutrients as U. prolifera was declining. The contribution of the U. prolifera bloom to atmospheric DMS was estimated to be 125 kg S d−1.