Metal isotopes are versatile pollutant source trackers, but biogeochemical processes can overprint or alter the original source isotopic fingerprint and thus hinder contamination tracing. Here, we explore Fe isotope systematics for the complete range of natural and metallurgical processes related to Ni lateritic ores from Barro Alto, Brazil, to assess its potential as a tracer in polluted lateritic soil contexts developed in an ultramafic system.The homogeneous δ57Fe values from protolith to soil confirmed that no significant Fe isotopic variation occurred during the formation of the deep lateritic profile. In addition, no Fe isotopic fractionation was found during the smelting process. Although the δ57Fe values resulting from mining activities fall within the range of terrestrial sample signatures, the conservation of the δ57Fe values from the ores to the by-products is an advantage for tracing anthropogenic sources when (i) the pyrometallurgical plant uses feeding material with Fe ores imported from other geological formations exhibiting different δ57Fe values and/or (ii) the by-products are transported or dispersed to other locations with different δ57Fe signatures in the topsoil.