Pylori IgM Research Articles

THE EFFECT OF HELICOBACTER PYLORI ON INTERLEUKIN-6 IN PATIENTS WITH ISCHEMIC HEART DISEASE

Several studies have shown that the presence of Helicobacter pylori (H. pylori) may be a risk factor for Ischemic Heart Diseases (IHD). Therefore, it is needed to look into the relation between IHD and H. pylori infection. One hundred Iraqi patients with IHD and 60 healthy Iraqis as a control group participated in the current study. The ELISA assay was used for the measurement of the sera levels for anti- H. pylori IgG, IgM, and Interleukin-6 (IL-6). Also, H. pylori DNA in the human venous blood was investigated by the polymerase chain reaction (PCR) using 16S rDNA primers. Between IHD patients and the control group, H. pylori-IgG and IL-6 mean ± SD were (Patient =1.814±0.08, Control = 0.823±0.09, Patient = 49.73±6.60, Control = 0.21±0.02, respectively), and this results showed a highly significant variation (p < 0.05) in anti-H. pylori IgG and IL-6, while no significant variations (p > 0.05) were found in anti- H. pylori IgM. It was also found that PCR amplification of Helicobacter pylori DNA using 16S rDNA primers was positive in the venous blood of 81 (81%) IHD patients. The current study results proposed that there was a relation between H. pylori and Ischemic Heart Disease.

The impact of oral ciprofloxacin on the structure and functions of rat gastric mucosa

Ciprofloxacin (CPX), is a fluoroquinolone antibiotic used to treat a number of gram-negative and gram-positive bacterial infections. Ciprofloxacin can cause severe side effects, ranging from tendon problems, nerve damage, to serious mood or behavior changes.The purpose of this study was to investigate how ciprofloxacin affects gastric cell lines in rats with a distinctive emphasis on physiological, histopathological, and bacteriological changes. Male albino rats (n = 21) were distributed into three groups; control, CPX, and CPX-withdrawal groups. The treated rats were given CPX tablets (12.5 mg/kg) dissolved in carboxymethyl cellulose (CMC) 0.5% orally once daily via gavage for sixty consecutive days. Control rats received only the vehicle. The withdrawal group was treated for 60 days and the drug was withdrawn for another sixty days. After completion of the experiment, all rats were sacrificed and gastric tissues were treated for light, immunohistochemical, and scanning electron microscopic examination. Image J software was used to measure immune-labeled gastric epithelial cells. Blood samples were also collected for H. Pylori immunoglobulins IgM, IgA, and IgG. Results showed that treated rats acquired significantly strongly positive tumor necrosis factor (TNFα) and significant reduction of serum level of H. pylori IgM, IgA, and IgG in all the study groups. It could be concluded that prolonged oral CPX administration to albino rats changes the gastric mucosal architecture and bacteriology.

The value of two onset determination of anti-H.pylori IgM antibody in patients with dyspepsia in Iraq

The value of two onset determination of anti-H.pylori IgM antibody in patients with dyspepsia in Iraq

The prevalence of Helicobacter pylori cagA (+ve) among patients with gastric cancer: an Egyptian study

Introduction and aimsGastric cancer is currently the second most common cause of cancer-related death in the world and the fifth most common cancer and the fourth leading cause of cancer-related death in Europe. It has been evident for more than the past 20 years that Helicobacter pylori is involved in the development of gastric adenocarcinoma. The cagA gene of H. pylori is the main virulence factor that leads to the development of gastric adenocarcinoma through the derangement of cellular architecture and signaling. The objective of our work is to study the prevalence of cagA among patients with gastric cancer.Patients and methodsThis descriptive study was done on 60 patients with gastric cancer underwent serum anti-H. pylori IgM and anti-cagA IgG assessment, computed tomography, upper endoscopy, and biopsy taking, and if needed, computed tomography-guided biopsy, followed by histopathological examination.ResultsA total of 34 (56.67%) patients were cagA +ve and 26 (43.33%) patients were cagA -ve, with no statistically significant difference regarding sex or age.ConclusionH. pylori cagA plays a significant role in development of gastric cancer, so we recommend not only H. pylori screening but also cagA virulence strain.

Seroprevalence of Helicobacter Pylori Infection in Iranian Adolescents: the CASPIAN- III Study

Background: Helicobacter pylori (H.pylori) is a common bacterial infection, with considerably high morbidity and mortality worldwide. This bacterium represents a key factor in the etiology of various chronic infections ranging from gastritis, peptic ulcer disease to gastric cancer; but the prevalence has large variations in different communities. The aim of this study was to estimate the prevalence H. pylori infection in a nationally representative sample of Iranian adolescents.Materials and Methods: In this cross-sectional study, serum samples of 882 Iranian adolescents, aged 10-18 years, were examined for seroprevalence of H.pylori. They were randomly selected from the samples obtained in the third survey of a national surveillance program (the CASPIAN III study). Seroprevalence of H. pylori was examined by detection of H. pylori immunoglobulinA (IgA), immunoglobulinG (IgG) and immunoglobulinM (IgM) in sera by using Enzyme Linked Immunosorbant Assay (ELISA).Results: The study participants had a mean age of 14.82 + 2.77 years. Overall, 51.7% of students were boys and 61.52% were urban residents. The H. pylori IGM and IGA seropositivity had no significant association with demographic characteristics (p>0.05). The H. pylori IgG seropositivity were significantly different in boys and girls (69.7%, 95% confidence interval [CI] = 66.7-72.7 vs. 76.3%, 95%CI= 73.5-79. 1, respectively, P=0.03).Conclusion: The seroprevalence of H. pylori IgG in Iranian adolescents is high, and girls had greater risk of H. pylori IgG seropositivity compared to boys. Preventive strategies and health education are recommended to reduce the prevalence of this infection in Iranian adolescent.

High Levels of IgA Antibodies to Helicobacter Pylori among Omani Women during Pregnancy and after Delivery.

Helicobacter pylori (H. pylori), is a common infection in pregnant women accompanied by variations in the levels of the IgM, IgA and IgG antibody isotypes. The variations of anti-H. pylori antibodies during and after pregnancy, and the extent of protection they provide to the mother and the fetus are not completely understood. To investigate the changes of the anti-H. pylori IgM, IgA and IgG levels in healthy Omani pregnant women during pregnancy and 3 months after delivery. Serum samples obtained from 70 Omani healthy pregnant women, with no history of autoimmune diseases, were tested for anti-H. pylori IgM, IgA and IgG in the first trimester of pregnancy and 3 months after delivery. In parallel and as a control group, sera obtained from a group of 70 healthy non-pregnant Omani women were tested. The levels of anti-H. pylori IgM, IgA and IgG were measured using standard Enzyme Linked Immunosorbent Assays (ELISAs). Anti-H. pylori IgA levels were found to be significantly higher during pregnancy (p=0.046) and after delivery (p=0.02) when compared to the control group. Moreover, a significant increase in the levels of anti-H. pylori IgM, IgA and IgG was detected after delivery (p=0.002) when compared to the levels during pregnancy. Pregnancy is associated with an increase in the levels of anti-H. pylori IgA antibodies. In addition, anti-H. pylori IgM, IgG and IgA antibody levels increase after delivery.

Detection of Helicobacter pylori IgG and IgM Antibodies in Iraqi Dyspeptic Patients

This study is conducted to detect H. pylori IgG and IgM antibodies by different serologicaltechniques (Immunochromatography test for rapid H. pylori IgG antibodies detection andenzyme linked immunosorbant assay test for H. pylori IgM antibodies detection). One hundredand twenty five dyspeptic patients are subjected to esophageal gastroduodenoscopy at theEndoscopy Unit at Baghdad Teaching Hospital from 10/1/2013 to 8/1/2014. Venous bloodsamples are collected from each patient. This study recorded, (89%) sensitivity for each of IgMTest by ELISA kit and Rapid IgG Test by Immunochromatography kit, but Rapid IgG Test hasmore specificity (85.64%) than IgM Test (80.8%).The higher prevalence 31.2% of H. pylori IgMantibodies observed among age group (21-40) years, whereas the IgM showed a slight rise 2.4%from the (10-20) years age group as acute H. pylori infection, although statistically there is a nonsignificant (P ≥0.05) association between H. pylori IgM antibodies and gender, but the females(32%) were more affected than males (27.2%). Also recorded significant (P ≤0.05) associationbetween chronic H. pylori infection and gender (male 45% and female 55%), and highlysignificant association of blood groups phenotypes and Rh factor with H. pylori infectionrecorded by rapid IgG test (P ≤0.01), as the seropositivity of H. pylori according to rapid IgG testwas (35, 15, 0 and 50) % for (A, B, AB and O) patients respectively, and (85and 15) % for Rh+and Rh- patients, respectively.

The Role of Serological Tests in Diagnosis of Helicobacter pylori Infection in Children

Background: Helicobacter pylori infection is a common infection that affects human beings. This infection also affects children. Different diagnostic methods such as serology, stool antigen detection, rapid urease test and histology are used to detect this microorganism. Objectives: The aim of this study was to determine the correlation between serology and histology/rapid urease test. Patients and Methods: In this study, two groups (case and control) were selected and matched for age and sex. The case group comprised of 77 children with confirmed H. pylori infection, as they had positive rapid urease test and histology results. The control group included 77 healthy children. Both case and control groups were checked serologically for detection of anti H. pylori IgM, IgG and IgA antibody titers. Receiver operating characteristic (ROC)-analysis software was used for data analysis. Results: Amongst the case group 54.6% were female and 45.4% were male. The most common complaints were abdominal pain (96%) and anorexia (82%). Using ROC-analysis method three cut-off points for IgG, IgM and IgA were obtained. These points were 3.3 U/ML for IgA, 6.4 U/ML for IgM and 9.9 U/ML for IgG. Considering cut-off points for each antibody, higher levels were considered as positive and lower levels as negative. Antibody titers were compared with gold standard methods including histologic and rapid urease tests. IgA level had a sensitivity of 64%, specificity of 58%, accuracy of 59.3%, positive predictive value of 31.5% and negative predictive value of 76.9%. IgM level had a sensitivity of 76%, specificity of 36.1%, accuracy of 74.2%, positive predictive value of 31.5% and negative predictive value of 76.9%. IgG level had a sensitivity of 58.6%, specificity of 61.3%, accuracy of 60.6%, positive predictive value of 36.9% and negative predictive value of 79.3%. Conclusions: Therefore, these antibodies have a relatively high negative predictive value and a low positive predictive value. Thus, their negative results are more valuable. The most sensitive antibody is IgM and most specific antibody is IgG. However, the performances of all serological tests for H. pylori are poor in children and these tests should not be used for diagnosis of H. pylori infection and treatment decisions for the pediatric age group.

Frequency of H. pylori Antibodies among Patients with Gastrointestinal Symptoms Attending Khartoum Teaching Hospital- Sudan

A total of 90 patients with gastrointestinal symptoms, and who attended Khartoum Teaching Hospital during the period from April to October 2012, were enrolled in this study. Out of them 35 (38.9%) were males and 55 (61.1%) were females, their age ranges from 15-60 years with mean (33.2). The aim of this study was to detect frequency of Helicobacter pylori IgM, IgG antibodies, and to determine the relationship between the presence of antibodies and certain factors such as (sex, age and genetic susceptibility). 90 serum specimens were collected from patients, and analyzed by ELISA technique. The results showed that 19 (21.1%), 57 (63.3%) were positive for IgM antibody and IgG respectively, while 15 (16.7%) were positive for both. Females were more affected than males, high frequency of positive results was observed among 15-30 age groups. Statistical analysis showed that there was insignificant correlation between age, gender, genetic susceptibility and presence of Helicobacter pylori antibodies (P >0.05).

Seroprevalence of anti-Helicobacter pylori antibodies in population of Sulaimani governorate/Kurdistan Region/Iraq

Helicobacter pylori (H. pylori) infection is one of the most common chronic bacterial infection in the world. Objective: This study was done to assess the extent of H. pylori infection in Suleimani governorate by noninvasive ELISA technique for quantitative detection of anti-H. pylori IgM, IgG and IgA antibodies in serum. Methods: The study included 335 adult and children volunteers from Chamchamal and Sulaimani cities in Kurdistan region / Iraq. The participants answered a structured questionnaire and a blood sample was taken for serological analysis from each participant. The level of serum antibodies for IgM, IgG and IgA were detected using ELISA technique. Results: In the total sample 20.4% had positive IgM, 32.3% positive IgG and 58.2% positive IgA. Two hundred thirty four (74%) subjects had one or more of the antibodies, 138 subjects (41.1%) had at least one antibody positive (IgM 11 (3.2%), IgG 35 (10.4%), IgA 92 (27.4%). In this study 20% had at least two antibodies positive {IgM 25 (7.4%): IgG 45 (13.4%): IgA 64 (19.1%)}. Conclusions: H. pylori infection is common in population of Sulaimani governorate. IgA anti-H. pylori is the most prevalent antibody of population in Sulaimani governorate. The age group of 20-30 years old has the most seroprevalence of antibodies specific to H. pylori.

717 Correlation Between Helicobacter Pylori Serologic Tests with Rapid Urase and Histology in Children

BackgroundHelicobacter pylori infection is a common infection that affects the human being. This infection also affects the children. Different diagnostic methods such as serology, stool antigen detection, rapid urease test...

Is There Any Relationship Between Asthma and Asthma Attack in Children and Atypical Bacterial Infections; Chlamydia Pneumoniae, Mycoplasma Pneumoniae and Helicobacter Pylori

Asthma is a chronic inflammatory airway disease characterized by variable airway obstruction and bronchial hyperresponsiveness. There are many factors affecting the development and severity of childhood asthma such as genetic predisposition, atopy, environmental factors, obesity, diet, socioeconomic status, and infectious triggers. In the present study we aimed to investigate the frequency of Mycdoplasma pneumoniae, Chlamydia pneumoniae, and Helicobacter pylori infections in asthmatic children. We investigated also whether there is a relationship between these agents and asthma attacks. Seventy-nine asthmatic children (46 males, aged 5-15 years) were included in study. The study group was divided into two groups: group 1 consisted of 37 children with asthma attacks and group 2 consisted of 42 children with stable asthma. As a control group we studied 36 healthy children. Pulmonary function tests, skin prick tests for common allergens were performed; serum total IgE, phadiatop, specific IgM and IgG antibody levels (ELISA) for M. pneumoniae, C. pneumoniae and H. pylori were measured in all patients. Mycoplasma IgM and Chlamidia IgM were positive in 8.1% (3 patients) and 18.9% (7 patients) of group 1 patients, respectively. There was a statistically significant difference for Mycoplasma IgM (p = 0.031) and Chlamidia IgM (p = 0.03) between group1 and other two groups. We have not found significant difference for M. pneumoniae IgG, C. pneumoniae IgG and H. pylori IgM and IgG among groups. M. Pneumoniae and C. Pneumoniae may play a role in development of asthma exacerbations in childhood. We could not find a relationship between H. Pylori and asthma.

Helicobacter pylori infection in epileptic patients

It is well known that there might be an epidemiological association between Helicobacter pylori infection and extraintestinal diseases. This study aimed at determining H. pylori infection in epileptic patients. Forty-seven cryptogenic epileptic patients (Group 1) and 35 healthy people (Group 2) participated in this study. Presence of H. pylori infection was examined by H. pylori stool antigen (HpSA), H. pylori IgG, and IgM. HpSA was detected in 21 participants (44.6%) in Group 1 and in 3 participants (8.5%) in Group 2. H. pylori IgM was positive in 27 participants (57.4%) in Group 1 and in 8 participants (22.8%) in Group 2. H. pylori IgG was positive in 37 participants (78.7%) in Group 1 and in 13 participants (38%) in Group 2. The difference of rates of HpSA, H. pylori IgM and IgG in Groups 1 and 2 were found statistically significant (chi2=4.18, p=0.04; chi2=9.18, p=0.0017; chi2=14.58, p<0.001, respectively). We also compared presence of H. pylori infection between the epileptic patients with poor and good prognosis; HpSA positivity was detected in 15 (62.5%) of 24 and 6 (26%) of 23, respectively, and the differences were statistically significant (chi2=6.30, p=0.012). H. pylori IgM positivity was detected in 16 (66%) of 24 patients with poor prognosis and 11 (47.8%) of 23 patients with good prognosis (p>0.05). H. pylori IgG positivity was detected in 18 (75%) of 24 patients with poor prognosis and 19 (82.6%) of 23 patients with good prognosis. The differences of H. pylori IgM and IgG positivity rates in epileptic patients with poor and good prognosis were not found statistically significant (p>0.05). These results suggest a probable association between the acute H. pylori infection and epilepsy, especially with poor prognosis.