Purification Efforts Research Articles

Highly enhanced fluoride removal from aqueous systems via solvent extraction utilizing trialkyl phosphine oxide as an efficient extractant

Solvent extraction (SX) has been recognized as an efficient approach for the large-scale removal of impurities, demonstrating considerable potential for fluoride extraction in industrial applications. However, existing fluoride extraction methods typically require the incorporation of additives to form complexes with fluoride for its removal. Due to the excessive additive requirements for efficient fluoride removal, these methods inevitably result in secondary contamination of feed solution, necessitating additional purification efforts to remove them. In this study, four extractants (trioctyl amine, tributyl phosphate, trioctyl phosphate, and trialkyl phosphine oxide (TRPO)) were investigated for fluoride removal via hydrogen bonding with hydrofluoric acid (HF). Various parameters, including the concentrations of extractants and stripping agents, pH values, extraction time, and temperature were initially optimized. It was found that TRPO exhibits the highest extraction efficiency for HF, with a single-stage extraction rate exceeding 70 %. Complete fluoride removal (> 99.9 %) was achieved through a consecutive four-stage extraction process. Further experiments, including maximum loading and slope analysis, combined with FT-IR and 19F NMR characterization, and DFT calculations elucidate the mechanism of the extraction. The transfer of HF into the organic phase is accomplished by the formation of a 1:1 hydrogen-bonded complex with TRPO. The loaded fluoride in the organic phase can be easily stripped by a base to get a fluoride salt. This study paves the way for the construction of novel SX systems to remove fluoride as HF.

Detection of Avian Leukosis Virus Subgroup J (ALV-J) Using RAA and CRISPR-Cas13a Combined with Fluorescence and Lateral Flow Assay.

Avian Leukosis Virus (ALV) is a retrovirus that induces immunosuppression and tumor formation in poultry, posing a significant threat to the poultry industry. Currently, there are no effective vaccines or treatments for ALV. Therefore, the early diagnosis of infected flocks and farm sanitation are crucial for controlling outbreaks of this disease. To address the limitations of traditional diagnostic methods, which require sophisticated equipment and skilled personnel, a dual-tube detection method for ALV-J based on reverse transcription isothermal amplification (RAA) and the CRISPR-Cas13a system has been developed. This method offers the advantages of high sensitivity, specificity, and rapidity; it is capable of detecting virus concentrations as low as 5.4 × 100 copies/μL without cross-reactivity with other avian viruses, with a total testing time not exceeding 85 min. The system was applied to 429 clinical samples, resulting in a positivity rate of 15.2% for CRISPR-Cas13a, which was higher than the 14.7% detected by PCR and 14.2% by ELISA, indicating superior detection capability and consistency. Furthermore, the dual-tube RAA-CRISPR detection system provides visually interpretable results, making it suitable for on-site diagnosis in remote farms lacking laboratory facilities. In conclusion, the proposed ALV-J detection method, characterized by its high sensitivity, specificity, and convenience, is expected to be a vital technology for purification efforts against ALV-J.

Roles of Nitrogen- and Sulphur-Containing Groups in Copper Ion Adsorption by a Modified Chitosan Carboxymethyl Starch Polymer

Owing to the toxicity and widespread use of copper, the pollution caused by copper ions has become a long-standing environmental and industrial challenge. In this study, a new adsorbent was developed to dispose of and remove copper ions from water. The modified chitosan–carboxymethyl starch (MCTS-CMS) polymer was characterised, and FTIR and SEM-EDS confirmed the successful graft modification of the receptor. The adsorption behaviour was investigated through various parameters, and the results showed that the optimal parameters were pH > 4.0, an adsorption time of 30 min, a reaction temperature of 293 K, and an initial concentration of 100–120 mg/L. The experimental data exhibited a good fit with pseudo-second-order models, and the Langmuir isotherm revealed that the polymer was found to be highly suitable for adsorption, with a maximum adsorption capacity of 321.16 mg/g. Thermodynamic analysis revealed that the adsorption process was exothermic and spontaneous. XRD and XPS confirmed the generation of posnjakite after the adsorption and the predominant roles of nitrogen- and sulphur-containing groups in the adsorption. Further analysis confirmed the existence of chemisorption and physical adsorption, with chemisorption mainly facilitating the Cu(II) absorption of the polymer. MCTS-CMS showed an excellent removal efficiency of 98% in acidic solutions. On the basis of these findings, the MCTS-CMS polymer demonstrates excellent performance and high selectivity in the removal of copper ions from industrial wastewater or polluted water bodies. This work recommends expanding the polymer’s practical applications to contribute to water purification efforts.

Bioaerosol Inactivation by a Cold Plasma Ionizer Coupled with an Electrostatic Precipitator.

Despite best efforts in air purification, airborne infectious diseases will continue to spread due to the continuous emission of bioaerosols by the host/infected person. Hence, a shift in focus from air purification to bioaerosol inactivation is urgently needed. To explore the potential of the cold plasma technology for preventing rapid spread of airborne infectious diseases, we studied a cold plasma ionizer (CPI) device and an electrostatic precipitator (ESP)-coupled CPI (CPI-ESP) device for the inactivation and cleaning of surface-spread microorganisms and bioaerosols, using porcine respiratory coronavirus (PRCV), Escherichia coli (E. coli), and aerosolized E. coli as representatives. We firstly demonstrated that CPI coupled with ESP is an effective technology for inactivating virus and bacteria spread on surfaces in an in-house test chamber. We then demonstrated the efficacy of CPI-coupled ESP for the inactivation of aerosolized E. coli in the same chamber. Furthermore, we have demonstrated the efficiency of a CPI-ESP coupled device for the inactivation of naturally occurring airborne microbials in a few indoor settings (i.e., a living room, a discussion room, a schoolroom, and an office) to determine the treatment duration- and human activity-dependent efficacy. To understand the disinfection mechanism, we conducted a fluorescence microscopy study to reveal different degrees of E. coli bacteria cell membrane damage under CPI treatment.

Synthesis of dicholesteryl organogelator as a green sorbent nanomaterial for oil spill remediation

In the field of global water purification, the issue of marine oil spills represents a significant challenge. The use of phase-selective organogelators (PSOGs) as sorbent materials in oil spill remediation is a promising solution due to their environmental adaptability and high absorption capacity. However, there are limited reports on PSOGs that can be used in powder form for rapid phase-selective gelation of crude oils. In this context, the development of innovative dicholesteryl derivatives as low-molecular-weight organogelators (LMOGs) offers a promising solution in powder form. These gelators are synthesized through a one-pot multi-component reaction as green synthesis method, which ensures high purity and eliminates the need for harsh conditions. The incorporation of cholesterol into the gelator structure demonstrate environmental adaptability. The exceptional sorption capacity was attributed to the structured 2D/3D networks observed through scanning electron microscopy (SEM). The hydrophobic properties of these gelators, as evidenced by a water contact angle of 118 degrees, enable them to efficiently gel various organic solvents at low concentrations (1% w/v) at ambient temperatures, without the need for heating–cooling cycles or co-solvents. The eco-friendly nature and efficient oil–water separation capabilities of these gelators in powder form represent a significant advancement in global water purification efforts.

Quantitative prediction of water quality in Dongjiang Lake watershed based on LUCC

Land Use/ Cover Change (LUCC) plays a crucial role in influencing hydrological processes, nutrient cycling, and sediment transport in watersheds, ultimately impacting water quality on both spatial and temporal scales. Accurately predicting changes in watershed water quality is beneficial for the sustainable management of water resources. Current models often lack the ability to effectively predict water quality changes in a dynamic spatio-temporal context, particularly in complex watershed environments. The overall purpose of the study is to establish a comprehensive and dynamic modeling framework that links LUCC with water quality, allowing for accurate predictions of future water quality under varying land use scenarios. The model, which uses water quality as the dependent variable and LUCC as the independent variable, was developed to quantitatively predict changes in watershed water quality. To achieve this, annual multi-period remote sensing images from Landsat-5, Landsat-8 or Sentinel-2 satellites spanning from 1992 to 2022 were analyzed. Random Forest (achieving a Kappa coefficient of 0.9468) were employed to classify land use within the watershed. Based on classification results, a Cellular Automata-Markov chain model (CA-Markov) was constructed to simulate and predict the spatio-temporal patterns of land use, incorporating driving factors such as proximity to water systems, roads, elevation, and slope. Validation of the model using LUCC data from 2020 yielded a high prediction accuracy with a Kappa coefficient of 0.9505. The CA-Markov model was further utilized to project LUCC under three different scenarios—natural development, ecological protection, and arable land protection—between 2023 and 2033. Based on these projections, the coupled water quality and LUCC model was employed to predict water quality changes in the watershed over the same period. Key findings indicate that water quality is likely to improve under ecological protection scenario, while deterioration is expected under natural development scenario and cropland protection scenario due to urban expansion, agricultural practices, and water diversion for irrigation. This study provides a robust framework for watershed management, offering scientific guidance for source management and water purification efforts, thereby contributing significantly to the sustainable development of water resources.

Investigation of cellulosic fines removal in dissolving pulp production with small-holed screen plates

This study investigates the influence of hole diameter and hole spacing on the fractionation performance using an industrial scale pressure screen. The objective is to increase the quality of the long fiber fraction during dissolving pulp production by separating cellulosic fines from unbleached softwood sulfite pulp containing 8% (w/w) primary fines (fibrous particles shorter than 0.200 mm fiber length). Different screen basket designs were applied, using the same hole spacing while varying hole diameter, and vice versa. This allowed a comparison of the separate influence of hole diameter and hole spacing on the separation efficiency. Production parameters of screening devices are always a compromise between separation efficiency, runnability/plugging, and throughput. High aperture velocity, high feed consistency and low reject rate were identified as the crucial operating parameters for high cellulosic fines removal efficiency, which turned out to be positive in terms of throughput. Considering the different hole diameters, the industrial trials showed that smaller holes yielded better fractionation results. Whereas reduced hole spacing resulted in a denser fiber network on the screen, reducing the amount of fines and short fibers passing, thereby leading to worse fractionation results. By choosing suitable screening conditions, we found that the weight-weighted fines content was reduced more than 50 percent using a one stage fiber fractionation screen set up under industrial conditions. The lowered fines content in the long fiber fraction is expected to allow a reduced purification effort and a lower chemical demand in the subsequent processes of dissolving pulp production. By reducing the fines fraction, consisting of short fibers, fiber fines, as well as mainly short-chain alkali-soluble carbohydrates (hemicelluloses), improvements in process efficiency and dissolving pulp quality can be achieved.

Ibuprofen removal from water using the IB-COF covalent organic framework

The rising presence of ibuprofen (IBP) in natural water bodies, restricting from its widespread pharmaceutical usage, necessitates effective remediation strategies. This study introduces IB-COF, a novel covalent organic framework synthesized via a solvothermal method, specifically engineered for IBP extraction from aqueous solutions. IB-COF showcases remarkable adsorption performance, achieving equilibrium within 60 min with a capacity of 512 mg/g, outperforming conventional adsorbents. Its adsorption kinetics align with pseudo-second-order and Langmuir models, indicating efficient monolayer adsorption. Significantly, IB-COF exhibits robust recyclability over five cycles. Among the prevalence of IBP contamination, IB-COF demonstrates promise in selectively extracting IBP even in the presence of competing pharmaceuticals. Overall, our findings underscore the potential of IB-COF as an advanced adsorbent for mitigating IBP pollution in water sources, contributing significantly to environmental purification efforts and water pollution mitigation strategies.

Decarboxylative Iodination of Coumarin‐3‐carboxylic Acids: A Facile Access to 3‐Iodocoumarins

A straightforward protocol for the direct conversion of readily available coumarin‐3‐carboxylic acids to the corresponding 3‐iodocoumarins is described herein. Heating acetonitrile solution of coumarin‐3‐carboxylic acids, molecular iodine and potassium hydrogen phosphate resulted in the decarboxylative iodination of coumarin‐3‐carboxylic acids via radical mechanism. Various electron rich and electron poor coumarin‐3‐carboxylic acids were compatible with the established reaction conditions and consequently afforded the corresponding 3‐iodocoumarins in (46‐93%) reaction yields with minimum purification efforts. The formed products serve as valuable precursors to streamline more complex organic compounds incorporating coumarin core.

Utilizing Pometia Pinnata leaf extract in microwave synthesis of ZnO nanoparticles: Investigation into photocatalytic properties

In this work, ZnO photocatalyst has been synthesized using matoa (Pometia pinnata) leaf extract under various microwave irradiation powers at 360, 540, and 720 Watts for 3 minutes on each. The UV-Vis absorption spectra of ZnO exhibited a peak in the ultraviolet region 300-360 nm. UV-Vis absorption analysis revealed a decrease in the band gap energy from 3.15 eV to 3.10 eV as the irradiation power increased. Field emission scanning electron microscopy (FESEM) images displayed spherical and nanoplatelet morphology with a decrease in particle size observed from 773 to 709 nm with increasing irradiation power. X-ray diffraction (XRD) analysis confirmed the hexagonal wurtzite structure of ZnO with crystallite sizes in the range of ~18-20 nm. The synthesized ZnO nanoparticles was successfully employed as a photocatalyst in 4-nitrophenol degradation, achieving the highest degradation percentage of 82.7% at 540 Watts with a corresponding reaction rate constant of 0.0126/min. In conclusion, the microwave-assisted synthesis of ZnO using on matoa leaf extract demonstrated significant potential for the degradation of organic pollutants, thereby contributing to water purification efforts.

Eco-Friendly Bio-Based Solvents for the Acetylation of the Amino Group of Amino Acids.

Nature-derived products, like juices and peel extracts of fruits and vegetables, have emerged in recent years as interesting and sustainable alternatives to traditional solvents in several synthetic applications. Herein, we present a green and fast method for the N-acetylation of amino acids, using several bio-based solvents (vinegar, tomato/kiwi/apple peel extracts, lemon juice, etc.). The high reactivity of the amino group is often a limitation in synthetic processes, making its protection a necessary step to achieve pure products and limit side reactions. Therefore, versatile, time-efficient procedures, minimal purification efforts, and good yields are desirable features for these transformations. Our new method meets all these criteria, offering a valuable and eco-friendly alternative to traditional approaches. In detail, we managed to obtain comparable yields to established setups, while improving safety and reducing the environmental impact of the overall process. Most notably, the milder conditions made it possible to avoid the use of running water (saving about 250 L/reaction) and electric-powered cooling devices.

The beneficial impact of kosmotropic salts on the resolution and selectivity of Protein A chromatography

During the manufacturing of therapeutic antibodies, effective Protein A chromatography as initial column step is crucial to simplify the remaining purification effort for subsequent polishing steps. This is particularly relevant for molecules with high impurity content so that desired product purity can be attained. The present study demonstrates beneficial effects on impurity removal when applying kosmotropic salts, e.g., sodium sulfate or sodium chloride, in the elution phase. Initially, a screen using negative linear pH gradient elution evaluated the impact of the kosmotropic salts in comparison to no additive and chaotropic urea using three mAbs and three common resins. Retaining acceptable yield, the kosmotropic salts improved resolution of monomer and impurities and reduced the contents of process-related host cell proteins and DNA as well as of product-related low and high molecular weight forms, despite some resin- and mAb-dependent variations. Moreover, a decrease in hydrolytic activity measured by a new assay for polysorbase activity was observed. In contrast, urea was hardly effective. The findings served to establish optimized step elution conditions with 0.25 M of sodium sulfate for a challenging mAb with complex format (bispecific 2 + 1 CrossMab) displaying high relative hydrophobicity and impurity levels. With yield and purity both in the range of 90 %, the contents of all impurity components were reduced, e.g., low molecular weight forms by two-fold and polysorbase activity by four-fold. The study indicates the potential of kosmotropic salts to establish efficient and comprehensive impurity separation by Protein A for facilitated downstream processing and economic manufacturing of complex antibodies.

Electrochemical Nitrate Reduction to Ammonia – Recent Progress

AbstractThe presence of nitrate in industrial, domestic and agricultural wastewater has a detrimental impact on both ecosystem and human health, as remediation and purification efforts are slow, challenging and difficult, given the high solubility and stability of this pollutant. Taking nitrate into high value‐added ammonia by electrochemical reduction can overcome high pollution and energy consumption limit of Haber‐Bosch process with long‐term significance of environmental protection and energy saving. This review summarized the research progress on the electrocatalytic reduction of nitrate to ammonia, with a focus on the reaction mechanisms, influencing factors, product detection methods, performance evaluation methods and the research status of electrocatalysts up to now. The review reported the latest strategies employed to design efficient electrocatalysts, such as pore structure regulation, alloying, heterostructure construction, defect and interface engineering, crystal surface regulation and microenvironment modulation of single‐atom catalysts. It highlights critical factors that determin the performance in terms of nitrate adsorption, exposed active sites, mass transfer rate, intermediates barrier and side reactions, as well as the stability of electrocatalysts and recovery of ammonia. In addition, the future direction of technology for electrocatalytic reduction of nitrate to ammonia has also been proposed.

Seed purity assessment of two local upland rice cultivars of Southeast Sulawesi based on agronomic characteristics

This research intended to purify upland rice seeds that have presumably been unintentionally mixed with various types of rice seeds, causing decreased quality or nature of the superior upland rice cultivars. Seed purification effort is needed to restore the purity of the seeds of promising local upland rice cultivars. The second long term goal of this research was to produce basic pure seeds, that are required for further breeding programs, and/or for the purpose of releasing new varieties. This study was the second generation of purification effort, aimed to further select the same or uniform agronomic characteristics of the local upland rice cultivars in each seed lot. Similar with the first generation, the experiment used a Randomized Block Design (RBD) consisting of 15 seed lots as treatment and each seed lot consisted of 4 “grids” (replications). This research was conducted at the Field Laboratory, Experimental Garden II, Faculty of Agriculture, Halu Oleo University. The results showed several characters (i.e., plant height, leaf number, and tiller number etc.) of the two rice cultivars were seem more uniform as compared to the first generation of purification. This indicates that the seeds are gradually purer.

Calculating Required Purification Effort to Turn Source Water Into Drinking Water Using an Adapted CCME Water Quality Index

AbstractThe 2000 European Union Water Framework Directive (WFD) states that “Member States shall ensure the necessary protection for the bodies of water identified with the aim of avoiding deterioration in their quality in order to reduce the level of purification treatment required in the production of drinking water.” However, it does not specify how to evaluate or quantify this level of purification treatment. We propose a novel water quality index (WQI) to quantify the level of purification treatment required to prepare drinking water from source water. It is based on the WQI of the Canadian Council of Ministers of the Environment (CCME WQI). Our WQI compares measured contaminant concentrations in source water to drinking water guidelines and any exceedance is weighted by a measure of the resilience of that contaminant to the treatment processes, which is not possible in the CCME WQI. Furthermore, it accommodates for varying sampling frequencies that are characteristic of the ongoing monitoring program. These adaptations make our index robust and sensitive to relevant changes in source water quality. We calculated index scores for source water from the Rhine and the Meuse and found no general decrease in required purification treatment levels since the introduction of the WFD.

A Polyketide Synthetase Gene Cluster Is Responsible for Antibacterial Activity of Burkholderia contaminans MS14.

Burkholderia contaminans MS14, isolated from a soil sample in Mississippi, is known for producing the novel antifungal compound occidiofungin. In addition, MS14 exhibits a broad range of antibacterial activities against common plant pathogens. Random mutagenesis and gene complementation indicate that four genes are required for antibacterial activity of strain MS14 against the fire blight pathogen Erwinia amylovora. With the aim of finding the biosynthetic gene cluster for the unknown antibacterial compound, we used RNA-seq to analyze the transcriptome of MS14 wild type and mutants lacking antibacterial activity. The twofold lower expressed genes in all mutants were studied, and a polyketide synthase (PKS) gene cluster was predicted to be directly involved in MS14 antibacterial activities. The nptII-resistance cassette and CRISPR-Cas9 systems were used to mutate the PKS gene cluster. Plate bioassays showed that either insertion or frame-shifting one of the PKS genes resulted in a loss of antibacterial activity. Considering that the antibacterial-defective mutants maintain the same antifungal activities as the wild-type strain, the results suggest that this PKS gene cluster is highly likely to be involved in or directly responsible for the production of MS14 antibacterial activity. Purification efforts revealed that the antibacterial activity of the compound synthesized by the gene cluster is sensitive to UV radiation. Nevertheless, these findings have provided more insights to understand the antibacterial activity of strain MS14.

Pengaruh Penyemaian yang Berbeda Terhadap Pertumbuhan Tanaman Kelor (Moringa oleifera)

Moringa (Moringa oleifera Lam.) is a type of tropical plant that is easy to grow in Indonesia. This plant is starting to be in great demand by the public and one of the types of plants that can be used as potential food. Its high nutritional status and benefits have earned it the nickname Mother's Best friend and Miracle tree. Other benefits of the Moringa plant are as an antimicrobial found in the seeds of the Moringa plant, coagulants in surface water purification efforts (pond water, river water, lake water) and a potential source of biodiesel raw material after jatropha curcas. Moringa cultivation can be done vegetatively and generatively. In this research, we will observe the generative reproduction of Moringa plants, namely through seeds. This study aims to find out how to sow moringa seeds that can produce the best plants. The treatment of soaking seeds in warm water showed a higher percentage of germination compared to immersion in ordinary water. The treatment of soaking the seeds in warm water followed by planting the sprouts in soil media added with manure had a very significant effect on plant height, number of leaves and root length.

Oil Palm Empty Bunches Adsorber Membrane for CO<sub>2</sub> Reduction of Biogas TPA Gunung Kupang in South Borneo

Biogas is one of the renewable energy alternatives. The heat of burning biogas depends on the percentage of CH4 (methane) gas and CO2 (carbon dioxide) gas. Biogas with high impurity gas components, such as CO2 and H2S (hydrogen sulfide), can cause a decrease in heating value. Therefore, purification efforts are needed. The adsorber membrane was successfully made from oil palm empty fruit bunches (OPEFB) which were pyrolyzed as physical activation. The adsorber membrane is activated to form a porous structure and a usable surface area for gas separation. This study aims to see the effect of variations in the carbon weight of OPEFB on reducing CO2 levels in the Gunung Kupang landfill biogas. Preparation of OPEFB adsorber membrane by mixing PVA (polyvinyl alcohol) and PEG (polyethylene glycol) polymers into OPEFB with varying weights, namely 12.5, 15, and 17.5 g (grams). Membrane performance is determined based on the results of the flame test and gas chromatography test results. Based on the results of the flame test of the biogas that has been passed on the adsorber membrane, overall all samples showed a predominantly bright blue flame color. Meanwhile, the chromatographic test results showed a decrease in CO2 levels in samples of 12.5, 15, and 17.5 g, respectively 19.6%, 32.02%, and 61.58%. The results of the flame test and gas chromatography test prove that the difference in mass weight affects the performance of the samples tested where the greater the mass of adsorber membrane used, the greater the increase in absorbed CO2 gas content.

Da’wah Thoughts of Hasan Al Banna and Said Nursi: A Comparative Research in Islamic Education Perspective

Islamic education has been strongly emphasized in Muslim communities. The quality of education and adherence to the Islamic worldview will shape Muslim personalities towards the true meaning of excellence in this world and the hereafter. This article discusses and introduces the efforts of Tawhid purification in Islamic Education perpsective undertaken by Hassan Al Banna (1906-1949 AD) and Badiuzzaman Said Nursi (1877–1960 AD) to address emergent problems within the Egyptian and Turkish Muslim community in early 20th century whereby the absorption of values and thought outside the authenticity of Islam had successfully influenced society thinking in terms of religious beliefs and practices. Both Hassan Al Banna and Said Nursi therefore offered a solution to this problem by implementing measures to purify tawhid in society. This article analyses their efforts in tawhid purification in the implementation of da’wah dictates in Egypt and Turkey. To achieve this goal, the historical analysis method is utilised to discuss the efforts of Hassan Al Banna and Said Nursi and present critical examination of his efforts to save the tawhid of Muslims and ensure they return to the true teachings of Islam. The results of this analysis highlight Hassan Al Banna and Badiuzzaman Said Nursi as a da’wah figure who contributed to tawhid purification in the Islamic world. The findings at the same time could be used as guidelines for the religious, preachers and researchers to propose tawhid purification as an important da’wah effort in society today.

Primary amines from lignocellulose by direct amination of alcohol intermediates, catalyzed by RANEY® Ni.

Primary amines are crucially important building blocks for the synthesis of a wide range of industrially relevant products. Our comprehensive catalytic strategy presented here allows diverse primary amines from lignocellulosic biomass to be sourced in a straightforward manner and with minimal purification effort. The core of the methodology is the efficient RANEY® Ni-catalyzed hydrogen-borrowing amination (with ammonia) of the alcohol intermediates, namely alkyl-phenol derivatives as well as aliphatic alcohols, obtained through the two-stage LignoFlex process. Hereby the first stage entails the copper-doped porous metal oxide (Cu20PMO) catalyzed reductive catalytic fractionation (RCF) of pine lignocellulose into a crude bio-oil, rich in dihydroconiferyl alcohol (1G), which could be converted into dihydroconiferyl amine (1G amine) in high selectivity using ammonia gas, by applying our selective amination protocol. Notably also, the crude RCF-oil directly afforded 1G amine in a high 4.6 wt% isolated yield (based on lignin content). Finally it was also shown that the here developed Ni-catalysed heterogeneous catalytic procedure was equally capable of transforming a range of aliphatic linear/cyclic primary/secondary alcohols - available from the second stage of the LignoFlex procedure - into their respective primary amines.