Pure Plantations Research Articles

Mixed Eucalyptus plantations enhance soil organic carbon accumulation and chemical stability through soil microbial community and multifunctionality

Forest conversion can impact soil organic carbon (SOC) fixation and ecosystem functions. The newly proposed “evenness of SOC chemical components” index can indicate the permanence of stored SOC during environmental disturbances. However, how the mixing of tree species impacts SOC accumulation and persistence and changes in soil microbial communities and multifunctionality remain unknown. Thus, we conducted a field experiment (11 years) of monospecific Eucalyptus urophylla × E. grandis plantation for evaluating the influences of the mixed planting of a non-nitrogen (N)-fixing tree, Castanopsis hystrix, and an N-fixing tree, Dalbergia odorifera, on SOC accumulation and chemical stability. Compared with pure plantations, mixed Eucalyptus plantations (particularly those mixed with D. odorifera) exhibited significantly increased SOC concentrations by 28.60–33.71 % and increased SOC chemical component evenness by 3.96–4.67 % (P < 0.05). We also found that the concentrations and chemical component evenness of SOC were strongly linked with litter quality, soil N, phosphorus and their availability, multifunctionality, microbial activity, carbon use efficiency (CUE), biomass, diversity, and network complexity. Furthermore, tree species mixing increased litter quality, soil nutrient availability, microbial biomass, activity, diversity, CUE, and network complexity. Soil microbial anabolism, community characteristics and multifunctionality influenced the SOC concentrations and SOC chemical components evenness. Specifically, microbial diversity enhanced the concentrations and chemical component evenness of SOC by increasing network complexity and soil multifunctionality. These findings revealed differential responses and mechanistic controls of SOC accumulation and chemical stability in different Eucalyptus planting patterns. Overall, mixed plantations, particularly those in which N-fixing species were introduced, effectively improved SOC accumulation and stabilization by positively altering soil quality and microbial properties, potentially representing a sustainable forestry management strategy to improve soil C benefits and multifunctionality in forest ecosystems.

Litter decomposition in pure and mixed plantations on the Loess Plateau, China: Lack of home-field advantage

Litter decomposition plays a critical role in the nutrient cycling of terrestrial ecosystems. According to the home-field advantage (HFA) hypothesis, litter decomposes more rapidly in its home habitat (where it originates) compared to other habitats (referred to as away). Although this hypothesis has been widely tested in natural forests, it remains controversial and lacks support for plantations, especially in dry ecosystems where microbial activities are limited. To clarify it, the present study investigated litter mass loss, nutrient dynamics, and decomposer communities in a 549-day reciprocal transplant experiment of litter decomposition in Robinia pseudoacacia (RP) and Hippophae rhamnoides (HR) pure plantations and their mixed plantations (5:5 and 8:2 ratios) on the Loess Plateau, China. The rate of mass, carbon (C), nitrogen (N) and phosphorus (P) loss in litter differed depending on litter type of species component and plantation type they were placed in. HR litter lost mass and released C, N, and P fastest, irrespective of plantation type. The overall mass loss of the four litter types were the fastest under 5:5 mixed plantations. However, litter did not decompose more rapidly in its home soil compared to the away soil. The structural equation modeling revealed that initial litter quality played a significant role in regulating the loss of C and N across decomposition stages, while the climate variations and microbial chemistry also contributed to the process. The similarity in soil microbial communities across plantation types, along with the limited interaction between litter and microbes in arid environments, could help elucidate the lack of HFA, with other factors such as the litter type exerting a minor influence. In summary, this study does not substantiate the hypothesis of home-field advantage but supports its context-dependency. Thus, regional heterogeneity should be taken into account when predicting the carbon cycle of forest ecosystems in future studies.

CONDITIONS OF THE OAK FORESTS GROWTH AND CULTIVATION ON THE TOP SURFACES AND SLOPES OF THE NEAR-VOLGA AND ERGENI UPLANDS AND THE PLAINS OF THE SAL-MANYCH INTERFLUVE

Planted oak forests in the south of European Russia are of great conservation and economic importance. The widespread practice of their creation in dry steppes and semideserts dates back to the 1950-1970s and allows to form oak (Quercus robur L.) plantations with longevity up to 40-60 years. The aim of the study is to investigate the growth features of oak trees under different soil-climatic and biocenotic conditions and to suggest methods to increase their longevity.The research was carried out in 2011-2016 in the south of the Near-Volga Upland, the northern part of the Ergeni Upland and lowland plains of the Sal-Manych interfluve at 28 temporary sample plots using common methods of forest inventory and regression data analysis.It has been established that the viability of oak is limited by unfavorable topographic position of plantations, increased climate dryness (HC 0,4-0,6), high content of soil carbonates and shallow-lying horizon of their accumulation, as well as the presence of shrub layer and other competitors for moisture. Under these conditions, afforestation of depressions on the plains and shady slopes with pure plantations of oak and other species without shrubs should gain advantage. The methods of their creation should be modified depending on the topographic position of particular forest area and the climate dryness.So, the main soil cultivation in depressions could last 1-2 years, applying the system of black fallow with plantation plowing. It is advisable to keep 3,5-4,0-meter row spacing, frequent tending, and intensive clearing of young tree stands. Maturer plantations need careful sanitary-selective cuttings, mainly the low-type ones, and preservation of high canopy density. Poor water availability and deterioration of plantations should be prevented by construction of dams.Reclamation plantations of low marketability are created on flat areas after 2-3 years of deeper basic tillage. The carbonate horizon should be loosened and a large moisture reserve is needed. Row spacing of 2,5-3,0 meters is reasonable, accelerating the canopy closure. The area of tree nutrition is expanded by low-intensive uniformly selective cuttings, forming more open stands. The longevity of oak in already existing plantations could be increased by eliminating its competitors, such as living ground cover, shrubs and associated species.

Tree species mixing effects on the radial growth of Pinus massoniana and Castanopsis hystrix: A comparison between even-aged and uneven-aged stands

The establishment of mixed-species forests with complex structures and high biodiversity has emerged as a key approach for enhancing productivity and maintaining the stability of plantation forests. However, how the mixing effect changes with stand development and climate variation is still unclear. Additionally, little is known about how the mixing effects vary between even- and uneven-aged mixed planting strategies based on the same species combinations and site conditions. In this study, we used dendrochronological methods to reconstruct 37 years of annual growth series for even-aged mixed plantations (EMs) and uneven-aged mixed plantations (UEMs) of Pinus massoniana and Castanopsis hystrix, as well as their respective monocultures at the individual tree (286 trees in total) level. We found that in EMs, the growth of P. massoniana and C. hystrix was 20.8 % lower and 26.2 % higher than those of their monocultures, respectively. However, the growth of P. massoniana remained unchanged during the first 17 years of mixing, after which it was significantly suppressed by C. hystrix. The growth of P. massoniana and C. hystrix in the UEMs was 20.8 % and 26.2 % lower than that in their monocultures, respectively. Remarkably, the mixing effect on the growth of P. massoniana shifted from suppression to promotion after 30 years of mixing. Furthermore, the relative growth of P. massoniana in the UEMs increased with dry season precipitation. These results indicate that transforming pure plantations into uneven-aged mixed plantations may be a crucial forest management strategy for enhancing tree growth and to improving adaptation to future precipitation pattern changes. However, in EMs, thinning should be conducted before fast-growing, shade-intolerant species experience intense light competition.

Community assembly of ectomycorrhizal fungal communities in pure and mixed Pinus massoniana forests

Ectomycorrhizal (EcM) fungi play an important role in nutrient cycling and community ecological dynamics and are widely acknowledged as important components of forest ecosystems. However, little information is available regarding EcM fungal community structure or the possible relationship between EcM fungi, soil properties, and forestry activities in Pinus massoniana forests. In this study, we evaluated soil properties, extracellular enzyme activities, and fungal diversity and community composition in root and soil samples from pure Pinus massoniana natural forests, pure P. massoniana plantations, and P. massoniana and Liquidambar gracilipes mixed forests. The mixed forest showed the highest EcM fungal diversity in both root and bulk soil samples. Community composition and co-occurrence network structures differed significantly between forest types. Variation in the EcM fungal community was significantly correlated with the activities of β-glucuronidase and β−1,4-N-acetylglucosaminidase, whereas non-EcM fungal community characteristics were significantly correlated with β-1,4-glucosidase and β-glucuronidase activities. Furthermore, stochastic processes predominantly drove the assembly of both EcM and non-EcM fungal communities, while deterministic processes exerted greater influence on soil fungal communities in mixed forests compared to pure forests. Our findings may inform a deeper understanding of how the assembly processes and environmental roles of subterranean fungal communities differ between mixed and pure plantations and may provide insights for how to promote forest sustainability in subtropical areas.

Synergistic variation of rhizosphere soil phosphorus availability and microbial diversity with stand age in plantations of the endangered tree species Parashorea chinensis.

Soil physicochemical properties and nutrient composition play a significant role in shaping microbial communities, and facilitating soil phosphorus (P) transformation. However, studies on the mechanisms of interactions between P transformation characteristics and rhizosphere microbial diversity in P-deficient soils on longer time scales are still limited. In this study, rhizosphere soils were collected from a pure plantation of Parashorea chinensis (P. chinensis) at six stand ages in the subtropical China, and the dynamic transformation characteristics of microbial diversity and P fractions were analyzed to reveal the variation of their interactions with age. Our findings revealed that the rhizosphere soils across stand ages were in a strongly acidic and P-deficient state, with pH values ranging from 3.4 to 4.6, and available P contents ranging from 2.6 to 7.9 mg·kg-1. The adsorption of P by Fe3+ and presence of high levels of steady-state organic P highly restricted the availability of P in soil. On long time scales, acid phosphatase activity and microbial biomass P were the main drivers of P activation. Moreover, pH, available P, and ammonium nitrogen were identified as key factors driving microbial community diversity. As stand age increased, most of the nutrient content indicators firstly increased and then decreased, the conversion of other forms of P to bio-available P became difficult, P availability and soil fertility began to decline. However, bacteria were still able to maintain stable species abundance and diversity. In contrast, stand age had a greater effect on the diversity of the fungal community than on the bacteria. The Shannon and Simpson indices varied by 4.81 and 0.70 for the fungi, respectively, compared to only 1.91 and 0.06 for the bacteria. Microorganisms play a dominant role in the development of their relationship with soil P. In conclusion, rhizosphere microorganisms in P. chinensis plantations gradually adapt to the acidic, low P environment over time. This adaptation is conducive to maintaining P bioeffectiveness and alleviating P limitation.

Litterfall-Associated Carbon Deposition and Vertical Profiles of Soil Organic Carbon in Different Land-Use Systems

Litterfall is one of the major inputs for soil nutrients. Understanding the connection of litterfall and soil organic carbon (SOC) as the part of ecological processes is a key step towards carbon sequestration as a climate change mitigation strategy. Yet, it remains inadequate to support by empirical pieces of evidence particularly in tropical ecosystems. In this study, litter traps were used to monitor the monthly organic carbon deposition over a year through litterfall, and soil samples were collected vertically up to 30 cm depth to define the SOC depth distribution in three different land use types located at Wondo Genet district, southern Ethiopia. The results were interpreted by deploying both the carbon stratification ratio (CSR) and carbon flow balance ratio (CFBR) as ecological indicators. The results revealed that both the annual litterfall amount and associated organic carbon input in plantation forest (958.4 ± 112 g·m−2·yr−1; 391.4 ± 112 g·C·m−2·yr−1) were higher than those in the homegarden (183.5.4 ± 26 g·m−2·yr−1; 67.4 ± 10 g·C·m−2·yr−1), conceivably due to few litter contributors (trees) present in the homegarden. The CSR of the homegarden (1.3 ± 0.01) was found between the ratio obtained for crop (1.2 ± 0.01) and plantation forest (1.4 ± 0.01), indicating that it is definitely a combination of pure plantation forest and crop system. The CFBR was higher in plantation forest (3.4 yr−1) than in soil of homegarden (0.77 yr−1), implying the net accumulation of soil carbon over time in the latter system. Hence, homegardens could be considered as a system of climate-smart practice with multiple-biogeochemistry pathways, which simultaneously address the social-absolute needs. Given the current tendency of transforming homegarden agroforestry to monoculture types owing to economical drivers, such indicators can dictate of making rational decisions related to land use planning and soil fertility management.

Growth and yield of Retrophyllum rospigliosii pure plantations in the Colombian Andes

Most reforestation in Colombia, especially in the Andean region, are monocultures of pines and eucalyptus with defined nursery and silvicultural packages. Planting native species would avoid the widespread controversy over reforestation with exotic species. Nevertheless, there are few studies that evaluate the growth and yield of native species and provide supported data to establish new projects. Here, we modeled the growth and yield of the native coniferous Retrophyllum rospigliosii in different areas of the Colombian Andes in terms of mean diameter (D), height (H), and volume (V) as a function of age, based on longitudinal data from 115 research sample plots from three sites that were remeasured between 4 and 9 times for 20 years. We fitted the von Bertalanffy growth model for D, H, and V through Bayesian generalized nonlinear mixed models to model tree growth. The adjustment of the models for the absolute growth of R. rospigliosii was 0.99, 0.99, and 0.97 for D, H, and V, respectively. All models fulfilled all regression assumptions. Although the growth rates of R. rospigliosii differed between planted sites, R. rospigliosii plantations generally exhibited low growth and barely reached the sizes required for commercialization. These results suggest that the extensive use of R. rospigliosii in pure plantations is unlikely and discourages the massive propagation of R. rospigliosii.

Characteristics of soil nitrogen and phosphorus fractions and microbial traits with increasing stand age in two-layered Cunninghumia lanceolata + Phoebe bournei plantations.

Soil nitrogen and phosphorus are two key elements limiting tree growth in subtropical areas. Understanding the regulation of soil microorganisms on nitrogen and phosphorus nutrition is beneficial to reveal maintenance mechanism of soil fertility in plantations. We analyzed the characteristics of soil nitrogen and phosphorus fractions, soil microbial community composition and function, and their relationship across three stands of two-layered Cunninghumia lanceolata + Phoebe bournei with different ages (4, 7 and 11 a) and the pure C. lanceolata plantation. The results showed that the contents of most soil phosphorus fractions increased with increasing two-layered stand age. The increase in active phosphorus fractions with increasing stand age was dominated by the inorganic phosphorus (9.9%-159.0%), while the stable phosphorus was dominated by the organic phosphorus (7.1%-328.4%). The content of soil inorganic and organic nitrogen also increased with increasing two-layered stand age, with NH4+-N and acid hydrolyzed ammonium N contents showing the strongest enhancement, by 152.9% and 80.2%, respectively. With the increase of stand age, the composition and functional groups of bacterial and fungal communities were significantly different, and the relative abundance of some dominant microbial genera (such as Acidothermus, Saitozyma and Mortierella) increased. The relative abundance of phosphorus solubilization and mineralization function genes, nitrogen nitrification function and aerobic ammonia oxidation function genes tended to increase. The functional taxa of fungi explained 48.9% variation of different phosphorus fractions. The conversion of pure plantations to two-layered mixed plantation affected soil phosphorus fractions transformation via changing the functional groups of saprophytes (litter saprophytes and soil saprophytes). Changes in fungal community composition explained 45.0% variation of different nitrogen fractions. Some key genera (e.g., Saitozyma and Mortierella) play a key role in promoting soil nitrogen transformation and accumulation. Therefore, the conversion of pure C. lanceolata plantation to two-layered C. lanceolata + P. bournei plantation was conducive to improving soil nitrogen and phosphorus availability. Bacteria and fungi played important roles in the transformation process of soil nitrogen and phosphorus forms, with greater contribution of soil fungi.

Under-canopy afforestation after 10 years: assessing the potential of converting monoculture plantations into mixed stands.

Under-canopy afforestation using different tree species is a key approach in close-to-nature management to improve the structural and functional stability of plantation forests. However, current research on understory afforestation mainly focuses on the seedling stage, with limited attention to saplings or young trees. In this study, we evaluated the growth characteristics and leaf traits of 14-year-old Pinus sylvestris var. Mongolica trees under four different upper forest density (UFD) treatments: 0 trees/hm2 (canopy openness 100%, CK), 150 trees/hm2 (canopy openness 51.9%, T1), 225 trees/hm2 (canopy openness 43.2%, T2), and 300 trees/hm2 (canopy openness 28.4%, T3). We found that the survival rate of P. sylvestris in the T3 was significantly lower than in the other treatments, with a decrease of 30.2%, 18.3%, and 19.5% compared to CK, T1, and T2, respectively. The growth of P. sylvestris in the T1 treatment exhibited superior performance. Specifically, T1 showed a significant increase of 18.8%, 5.5%, and 24.1% in tree height, diameter at breast height, and crown width, respectively, compared to the CK. The mean trunk biomass ratio in the understory was significantly higher than that in full light by 15.4%, whereas the mean leaf biomass ratio was significantly lower by 12.3%. Understory P. sylvestris trees tended to allocate more biomass to the trunk at the expense of decreasing leaf biomass, which would facilitate height growth to escape the shading environment, although the promotion was relatively limited. Leaf length, leaf width, leaf area, leaf thickness, mesophyll tissue thickness, epidermis thickness, and leaf carbon content were the highest in the CK and tended to decrease with increasing UFD, indicating that a high-light environment favored leaf growth and enhanced carbon accumulation. In summary, young P. sylvestris trees adapted to moderate shading conditions created by the upper canopy, and the T1 treatment was optimal for the growth of understory P. sylvestris. This study provides insights into different adaptive strategies of young P. sylvestris trees to changes in light environment, providing practical evidence for under-canopy afforestation using light-demanding trees during pure plantation transformation.

Responses of belowground fine root biomass and morphology in Robinia pseudoacacia L. plantations to aboveground environmental factors

The adaptation of fine roots to a variety of environmental conditions is crucial for promoting the growth and long-term survival of forest trees. To achieve sustainable forest ecology management requires a comprehensive understanding of the intricate relationships between belowground and aboveground factors in the forest ecosystem. Equally important is identifying the factors that directly affect the growth and development of fine roots belowground. This study was carried out in 25-year-old pure plantations of Robinia pseudoacacia L. within the Caijiachuan watershed, Jixian County, Shanxi Province, China. Twenty-seven permanent sample plots were established, with each plot measuring 20 × 20 m. These plots covered nine different stand densities ranging from 775 to 2975 trees/ha, with three replicates per density. Through field surveys and multivariate analysis, along with the use of a simplified Generalized Linear Model, this study revealed the alterations in belowground fine root biomass and morphology resulting from variations in stand density. Additionally, it explored the mutual relationships between belowground fine root biomass and morphology and aboveground environmental characteristics. The results show that, under different stand densities, only fine root biomass, specific fine root length, fine root tissue density, and fine root tip density displayed significant differences (P < 0.05), while specific fine root surface area exhibited a decreasing trend with increasing stand density. Moreover, lower densities (1025 and 1175 trees/ha) promoted the growth of specific fine root length and specific fine root surface area, while higher densities (2100 and 2475 trees/ha) supported the development of fine root biomass and fine root tip density. The combined contributions of semi-decomposition layer litter layer thickness (contribution value: 0.1340), mean tree height (contribution value: 0.2710), and neighborhood comparison (contribution value: 0.1840) significantly explain the variations in fine root biomass (P = 0.014) and specific fine root surface area (P = 0.031). Fine root biomass shows statistically significant correlations only with total phosphorus and available phosphorus (P < 0.05). The influence of soil factors (contribution value: 0.0306) on subterranean fine roots is much less pronounced compared to that of stand structure (contribution value: 0.1152) and other environmental factors (contribution value: 0.1128). These factors together account for the variations in subterranean fine roots, with stand structure and other environmental factors making the most significant contributions (contribution value: 0.5840). These research findings offer valuable insights into the connections between subterranean fine roots and aboveground biotic and abiotic factors in Robinia pseudoacacia L. plantations in the northern Loess Plateau region. Furthermore, they provide vital information for the prudent management and operation of low-efficiency Robinia pseudoacacia L. plantations.

Ecological stoichiometry of leaf-litter-fine roots in mixed plantations in mountainous area of Southern Ningxia, China.

The southern mountainous areas in Ningxia are representative regions of the Loess Plateau, with extremely fragile ecological environment. Large area of pure plantations established during the project of Grain for Green has suffered from poor nutrient availability and biodiversity loss, while planting mixed plantations is commonly consi-dered as an effective way to improve the ecological benefits. We selected Robinia pseudoacacia + Picea asperata mixed plantation, R. pseudoacacia + Armeniaca sibirica mixed plantation, A. sibirica pure plantation and R. pseudoa-cacia pure plantation located ina Ningnan mountainous area as test objects. Based on the theory and method of ecological stoichiometry, we measured the C, N and P contents of leaves, litter and fine roots to understand nutrient cycling characteristics of different plantations. The results showed that there was significant difference in foliar stoichiometry of each tree species within the four plantations. P. asperata leaves had the highest C content in the R. pseudoacacia + P. asperata mixed plantation, and R. pseudoacacia leaves had the highest N and P contents in the R. pseudoacacia + A. sibirica mixed plantation. N content of R. pseudoacacia and A. sibirica leaves was significantly higher in mixed plantation compared with that in pure plantation. There was no significant difference in litter biomass, litter C, N, P contents and stoichiometric ratios between the pure and mixed plantations of R. pseudoacacia. Litter biomass in A. sibirica pure plantation was significantly higher than that in R. pseudoacacia + A. sibirica mixed plantation, while litter C content was significantly lower than that in the mixed plantation. Fine root biomass decreased with increasing soil depth in the four plantations, with total fine root biomass being the highest in the R. pseudoacacia + A. sibirica mixed plantation. N content and N:P of fine roots in the R. pseudoacacia + A. sibirica mixed plantation were higher than those in R. pseudoacacia and A. sibirica pure plantations. There was significant negative correlation between N content in leaves and fine roots of R. pseudoacacia + A. sibirica mixed plantation. There were significant negative correlations between the N content of leaves and litter, as well as between the P content of leaves and fine roots in the R. pseudoacacia + P. asperata mixed plantation. P content between litter and fine roots in A. sibirica pure plantation was significantly negatively correlated. Nutrient status of mixed plantations was better than pure plantations in the Ningnan mountainous area, with the mixed plantation of R. pseudoacacia and A. sibirica being the best. Mixed planting reduced nutrient limitation on plant growth to a certain extent.

Impact of Simulated Acid Rain on Soil Base Cations Dissolution between Eucalyptus Pure Plantations and Eucalyptus–Castanopsis fissa Mixed Plantations

The soils of Eucalyptus pure plantations and Eucalyptus–Castanopsis fissa mixed plantations were studied using soil column leaching experiments with acid solutions to mimic the effects of acid rain on the soils. This helped researchers learn more about how soil base ions react to acid deposition and their ability to protect the soil from excessive acidity under pure and mixed-species plantations. The results showed that acid rain leaching increased the leaching loss, desorption, and desorption rate of soil base ions while decreasing the soil pH value, adsorption, and adsorption rate of soil base ions. The soil pH value and the leaching loss ranges of K+, Na+, and Mg2+ were all greater in the pure plantations than in the mixed plantations, while the leaching range of Ca2+ was greater in the mixed plantation than in the pure plantations. In the two types of plantations, the adsorption rates of Ca2+ and Na+ in the mixed plantations were higher than in the pure plantations, while K+ and Mg2+ showed higher adsorption rates in the pure plantations than in the mixed plantations. Therefore, soil pH and base ions were greatly affected by the pH value of acid rain. Compared with the pure plantations, the establishment of Eucalyptus–Castanopsis fissa mixed plantations can slow soil acidification and leaching of K+, Na+, and Mg2+ and contribute to the adsorption of Ca2+ and Na+, which is beneficial for the soil nutrient fixation of Eucalyptus plantations. The mixed plantations were found to increase the exchange reaction between H+ and base ions, thereby improving the acid buffer performance of the soil. This, in turn, helped to mitigate the decline in soil fertility. Therefore, establishment of Eucalyptus–Castanopsis fissa mixed-species plantations can slow down the impact of acid rain on soil acidification in artificial plantation land to a certain extent and play an important role in optimizing the plantation structure of Eucalyptus stands and maintaining their productivity.

Soil Organic Carbon Sequestration after 20-Year Afforestation of Mangrove Plantations on Qi’ao Island, Southern China

Mangrove afforestation is considered an important measure in the “natural-based solution” for mitigating climate warming through sequestering massive carbon (C) into vegetation biomass, yet how the planted mangrove species facilitate soil C sequestration remains unclear. Here, we investigated the stock, source, and fraction of soil organic carbon (SOC) over 1 m depth after 20-year afforestation of five mangrove pure plantations (Acrostichum aureum, Acanthus ilicifolius, Aegiceras corniculatum, Kandelia obovate, and Excoecaria agallocha) on Qi’ao Island, South China. The results showed that SOC stocks did not significantly differ among the five plantations, with an average value of 16.7 kg C m−2. Based on the two-end-member mixing model with plant–soil C stable isotope signatures, the autochthonous (mangrove-derived) C source accounted for 20.2–34.1% of SOC but varied significantly among the plantations. The SOC stock in particulate fraction (1.2–2.0 g C kg−1) and mineral-associated fraction (14.3–16.0 g C kg−1) also significantly differed among the plantations. The similar SOC stock but different source contributions and C fractions among the plantations observed here may have important implications for mangrove afforestation to optimize stand structure and maximize C sequestration.

Determinants of growth and carbon accumulation of common plantation tree species in the three northern regions, China: Responses to climate and management strategies

The Three-North (Northwest, North and Northeast) Shelter Forests Program (TNSFP) in China has effectively promoted vegetation growth and carbon sink in the temperate semi-humid and semi-arid regions. To compare the afforestation benefits of commonly used tree species in the area and explore the effect of environment on growth and carbon accumulation in plantations, backpack LiDAR was used to acquire 3 dimensional lidar point clouds of forests from a total of 480 pure plantation patches consisting of Pinus sylvestris (P.s.), Pinus tabuliformis (P.t.), Populus spp. (Pop.), and Robinia pseudoacacia (R.p.). Then, diameter at breast height (DBH), forest height, canopy coverage, and aboveground carbon accumulation were calculated for each plantation patches, which ranged from 7.0 to 37.3 cm, 1.5–14.5 m, 10–99 % and 4.2–205.9 Mg/ha, respectively. Generalized linear mixed-effect models and ANOVA were applied to account for the environmental constraints on the variations of forest parameters. Results showed that precipitation had a stronger effect on all the above parameters of plantations than temperature, and P.t. was more sensitive to climate than other three species. With regard to forest management in Pop. plantations, thinning could improve afforestation efficiency because carbon accumulation would reduce after the age exceeds 30 years. In contrast, P.s. populations maintained a continuous increase in carbon accumulation at least before 40 years old, while the radial growth of canopy became saturated after 12 years of age. The optimal planting density for P.s. and Pop. are about 1000 trees/ha, beyond which the increase in carbon accumulation will slow down or change rate of canopy coverage will be insignificant. Within the TNSFP area, P.t. and R.p. plantations would be more suitable in southern regions, while P.s. and Pop. plantations grow better in the northeastern regions. Meanwhile, mountains along the “Hu Line” showed high potential for growth and carbon accumulation for all tree species examined.

Effects of species mixtures on soil water storage in the semiarid hilly gully region

Mixed-species plantations are promoted to restore degraded ecosystems and improve soil quality worldwide. However, differences of soil water conditions between pure and mixed plantations are still controversial and how species mixtures affect soil water storage (SWS) was not well quantified. In this study, vegetation characteristics, soil properties and SWS were continuously monitored and quantified in three pure plantations (Armeniaca sibirica (AS), Robinia pseudoacacia (RP) and Hippophae rhamnoides (HR)) and their corresponding mixed plantations (Pinus tabuliformis-Armeniaca sibirica (PT-AS), Robinia pseudoacacia-Pinus tabuliformis-Armeniaca sibirica (RP-PT-AS), Platycladus orientalis-Hippophae rhamnoides plantation (PO-HR), Populus simonii-Hippophae rhamnoides (PS-HR)). The results found that SWS of 0–500 cm in RP (333.60 ± 75.91 mm) and AS (479.52 ± 37.50 mm) pure plantations were higher than those in their corresponding mixed plantations (p > 0.05). SWS in the HR pure plantation (375.81 ± 81.64 mm) was lower than that in its mixed plantation (p > 0.05). It is suggested that the effect of species mixing on SWS was species specific. Additionally, soil properties exerted more contributions (38.05–67.24 %) to SWS than vegetation characteristics (26.80–35.36 %) and slope topography (5.96–29.91 %) at different soil depths and the whole 0–500 cm soil profile. Furthermore, by excluding the effects of soil properties and topographic factors, plant density and height were particularly important to SWS (with standard coefficients 0.787 and 0.690 respectively). The results implied that not all the mixed plantations exhibits the better soil water conditions than the compared pure plantations, which was tightly related to species selected for mixing. Our study provides scientific support for revegetation technique improvement (structural adjustment and species optimization) in this region.

Stand characteristics and ecological benefits of Chinese Fir, Chinese Cedar, and mixed plantations in the mountainous areas of the Sichuan Basin

Many studies of biodiversity-ecosystem functioning (BEF) relationships indicate that mixed plantations can enhance ecological benefits by enabling ecological niche differentiation between species. However, few studies explore multi-dimensional evidence to fully evaluate the integrated ecological benefits of mixed plantations. This paper aims to assess the stand characteristics and ecological benefits of different forest types, drawing on multiple lines of evidence (trees, shrubs, herbs, soil fauna, and soils) to understand the effects of mixed plantations on integrated ecological benefits. Our study focused on pure Cunninghamia lanceolata (Chinese Fir) plantations (PCL), pure Cryptomeria fortunei (Chinese Cedar) plantations (PCF), and mixed C. lanceolata-C. fortunei plantations (MCC), which are common in the mountainous areas of the Sichuan Basin in southwestern China. We analysed the growth status, spatial structure, understory vegetation diversity, soil fauna characteristics, and physical and chemical soil properties found in plots representing these three forest types. The results showed that productivity was significantly higher in the MCC than in PCL or PCF, by 39.5% and 18%, respectively. Mixed plantations showed higher stand growth rates, but this effect was limited by interspecific competition. The results further showed that mixed plantations promote forest growth by allocating spatial locations between different tree species. In addition, the spatial stand structure in the mixed plantations was more stable than that in the two pure plantations, and the management potential of the mixed plantations was greater. Understory vegetation in MCC was significantly more diverse than in PCL and PCF, by 15.8–20% and 13.2–35.7%, respectively. Moreover, mixed plantations performed better in terms of soil water and soil fertility. Interestingly, we found no difference in soil fauna diversity between mixed and pure plantations. We conclude that, overall, planting mixed plantations can enhance the integrated ecological benefits of afforestation and may be an essential strategy for ensuring that plantations can cope with climate extremes.

Correlation between Changes in Soil Properties and Microbial Diversity Driven by Different Management in Artificial Chinese Fir (Cunninghamia lanceolata (Lamb.) Hook.) Plantations

Successive planting is the main pattern for cultivating Chinese fir (Cunninghamia. lanceolata (Lamb.) Hook.). However, the influence of this management has not been totally investigated, especially with respect to the changes in the soil microbial community and the relationship to soil properties. This study investigated the physical and chemical properties of the soil, its enzyme activities, and its microbial diversity in three adjoining plantations managed with different successive planting models (long-term continuous growth without harvest, M1; single harvest cutting followed by the construction of a pure plantation, M2; and double harvest cutting followed by the construction of a mixed plantation, M3) to evaluate the impact of these forest management practices. In most soil layers, M1 was observed to have significantly higher content of Na and Al ions, as well as more polyphenol oxidase (PPO) activity, and M2 had a significantly higher field moisture capacity (FMC) and content of Mg ions, while M3 had significantly higher urease (URE) activity. Changes in the totals of N (TN) and C (TC), alongside the availability of P (AP), C/P, N/P, URE, sucrose (SUC), and PPO values, correlated significantly with bacterial diversity, whereas the dynamics of total K (TK), Na, C/P, N/P, and PPO levels were significantly related to fungal diversity. Among the models, soil bacterial genera, including Burkholderia–Caballeronia–Paraburkholderia, Acidothermus, and Paenibacillus, were mostly affected by TN, TC, AP, organic matter (OM), C/N, C/P, N/P, SUC, and the performance of URE. The distribution of fungal genera in different models showed significant differences. Talaromyces, Trichoderma, and Aspergillus were relatively abundant in M1, while Umbelopsis and Saitozyma exhibited more adaptation in M3. These results illustrated better soil properties and higher abundance of microbial diversity in M1 and M3, and furthermore, demonstrated the strategic benefit of both prolonging the rotation period and of creating mixed artificial plantations to maintain diversity. This study improves the understanding of the impact of a successive planting strategy in C. lanceolata plantation sustainability.

Mixed-species plantations alleviate deep soil water depletion and facilitate hydrological niche partitioning compared to pure plantations

Understanding the effects of different silvicultural patterns on soil water reservoirs and how silvicultural species adjust their water use strategies and plant function traits is critical to guiding future silvicultural activities toward the sustainable development of plantations. We continuously monitored soil gravimetric water content (SWC), soil and plant isotopes, leaf water potentials at predawn and midday (Ψpd and Ψmd), and plant morphological traits in pure Populus simonii tree plantations (PPs), pure Amorpha fruticose shrub plantations (PAf), and mixed P. simonii-A. fruticose plantations (Msf) during the 2021 growing season. The study showed that the average SWC of the deep layer (80–200 cm) was Msf > PAf > PPs. P. simonii in the Msf increased the water use proportion of the deep layer compared to that in the PPs, especially during the vigorous growth period of June-July, and shifted the major water use from shallow (0–40 cm) to deep layers on average. A. fruticose mainly used shallow soil water during the growing season. The leaf-level water use efficiency of the same species did not change in the Msf compared to that in the PPs or PAf. Based on the slope (σ) of the linear equation of Ψpd-Ψmd, P. simonii and A. fruticose exhibited isohydric (σ < 1) and an-isohydric (σ > 1) behavior under varying soil water conditions, respectively. The wood density was higher and specific leaf area (SLA) was lower in the PPs than in the Msf for P. simonii, while the ratio of stem sapwood area to leaf area was higher and SLA was lower in the PAf than in the Msf for A. fruticose. These findings reflected that mixed tree-shrub plantations alleviate deep soil water depletion compared to pure plantations, facilitate hydrological niche segmentation of co-occurring species, and reduce carbon investment in xylem and leaves to resist water stress and possibly devote more resources to growth.

Multi-species plantation intensifies soil water competition and groundwater depletion in a water-limited desert region

Multi-species plantation has long been applied in forestry and landscape restoration to provide better ecosystem services and functions. However, the differences in water-use patterns between mixed and pure plantations are poorly understood, especially in the arid/semi-arid artificial desert ecosystems. The stable isotope compositions (δ2H and δ18O) of rainwater, xylem water, soil water, and groundwater were analyzed over three growing seasons (2019–2021) to identify the water-use patterns of Artemisia desterorum Spreng (A. desterorum), Amorpha fruticosea (A. fruticosa) and Pinus sylvestris var. mongolica (P. sylvestris) in pure and mixed plantations in the Mu Us Sandy Land. Seasonally, there was a transference of water-use pattern from deep (moist layer and groundwater) to shallow water sources (active and stable layers) during the transition period between the dry and rainy season for both pure and mixed A. fruticosa and P. sylvestris, which was attributed to dimorphic root systems of arbor and shrub in the water-limited region. However, A. desterorum with shallower roots predominately absorbed water from the active and stable layers across the whole growing season regardless of planting pattern. Annually, the three plant species in pure stands extracted water predominantly from the stable layer (36.97%–46.16%), followed by the active layer (23.55%–36.98%). In the mixed stand, however, all the plants absorbed water mainly from the active layer (33.04%–39.44%), followed by the stable layer (30.46%–33.68%), suggesting that multi-species plantation intensifies shallow soil water competition. Additionally, the proportional contribution of groundwater to plant water uptake under mixed plantation was higher than the pure plantations, causing faster groundwater decline in the mixed stand (0.29 m yr−1) compared to pure stands (0.11–0.20 m yr−1). Our study demonstrates that multi-species plantation in the water-limited desert region intensifies soil water competition and groundwater depletion and thus adversely affects sustainable development goals.