Pure Dosage Form Research Articles

Method Development and Validation of Salmeterol xinofoate by HPLC

A rapid and precise high performance liquid chromatography method has been developed for the validation of Salmeterol xinofoate in its pure dosage form. The separation was carried out on Agilent Zorbax Bonus RP- (250mm ×4.6mm 5μ) column with a mobile phase consisting of 0.1% Formic acid: Acetonitrile in the ratio of 64:36 v/v as a mobile phase and flow rate is 1ml/min. The detection was carried out at wavelength 234nm. The column thermostatically controlled at 30℃. The retention time of Salmeterol was found to be 1.96 min. The Salmeterol xinofoate followed linearity in the concentration range of 40-60μg/mL with r2= 0.999. The developed method was validated for sensitivity, accuracy and precision. The sample was scanned from 200- 400nm with PDA detector. The % recovery of sample was found to be. The LOD and LOQ of the Salmeterol xinofoate was found to be 2.67μg/ml and 8.08μg/ml respectively. The suitability of this HPLC method for quantitative estimation of Salmeterol xinofoate was proved by validation by the requirements of ICH guidelines.

Development of New Analytical Method for Estimation of Lansoprazole in Bulk and Tablet dosage forms

The present paper reports a simple, accurate and precise spectroscopic technique for estimation of Lansoprazole by first order derivative technique in tablet dosage form. The spectroscopic method for estimation of Lansoprazole by first order derivative technique was carried out using methyl alcohol as solvent. The absorbance maximum was 275nm. Beers law observed in the range of 30 - 150μg/ml concentration. The recovery studies demonstrated the accuracy of the proposed procedure and the results were established as per ICH guidelines. The technique was used successfully for the estimation of Lansoprazole in bulk and tablet and pure dosage form.

Spectrophotometric determination of methoxamine using cerium(IV) in presence of sodium lauryl sulphate and rhodamine-B

A sensitive spectrophotometric assay has been developed for the determination of methoxamine in pure dosage form and in its pharmaceutical preparations. The method is based on the acidic oxidation of methoxamine with cerium(IV) in the micellar medium of sodium lauryl sulphate at 96°C. The reaction yields a water-soluble purple product which can be quantified spectrophotometrically at 505 nm. The calibration curve was linear between 1.0 and 20 μg ml −1 with a limit of detection 0.5 μg ml −1. The molar absorptivity at 505 nm is 8.3 × 10 3 l mol −1 cm −1. The method is simple and rapid since the product is measured directly in solution without extraction.