The commercial-scale DeNOx catalyst cartridge coupled with filter bag have been developed for the removal of fine particulate matter and nitrogen oxides (NOx) in flue gases. The DeNOx catalyst cartridge coupled filter bag is made up of a microporous PTFE (polytetrafluoroethylene) membrane/impregnated polyimide fiber needle-punched pleated filter bag designed for the removal of ultrafine particulates, along with a pellet selective catalytic reduction catalyst-filled cartridge for the removal of NOx. V2O5-MoO3/TiO2 catalyst was used as the DeNOx catalyst. The distribution of cleaning air velocity inside the DeNOx catalyst cartridge-coupled filter bag, which is injected with compressed air from the high-volume low-pressure (HVLP) pulse injector to remove the deposited dust cake from the surface of the filter bags during pulse-jet cleaning, was confirmed using computational fluid dynamics (CFD). The particulate removal and dust cake dislodgement characteristics of the DeNOx catalyst cartridge coupled filter bag were tested in a commercial-scale filter bag test unit under conditions simulating fossil fuel combustion flue gas. The commercial-scale DeNOx catalyst cartridge coupled filter bag was 165 mm in diameter, 2000 mm in total length, and contained approximately 12 kg of DeNOx catalyst- filled cartridges. Nine commercial-scale DeNOx catalyst cartridge coupled filter bags were installed in the commercial-scale filter test unit. The experimental conditions were as follows: flue gas temperature 200 °C, flue gas flow rate 3000Nm3/h, NO, SO2, and particulate concentration in inflow flue gas 200 ppm, 7 ppm, and 5000 mg/Nm3 respectively, resulting in different values of filtration velocities. The efficiency of bag cleaning and intervals, overall collection efficiency, fractional efficiency, and filter quality were investigated. The average bag cleaning efficiency and overall collection efficiency were 95.5%, 91.5%, and 83.3% at filtration velocities of 0.8, 1.0 and 1.2 m/min, respectively. The corresponding values for overall collection efficiency were 99.9%, 99.5%, and 98.2%. So, both bag cleaning efficiency and overall collection efficiency decreased with increased filtration velocity. The present study confirmed that DeNOx catalyst cartridge coupled filter bags have relatively higher bag cleaning efficiency, collection efficiency, and filter quality for challenging fossil fuel combustion flue gas. Particularly, the fractional efficiency for micron-sized particles was considerably higher at lower filtration velocities.