Puff Development Research Articles

Sustainable Strategies for Increasing Legume Consumption: Culinary and Educational Approaches.

Legumes are nutrient-dense crops with health-promoting benefits. However, several barriers are associated with their consumption. Emerging issues including food neophobic tendencies or taboos, unclear dietary guidelines on legume consumption, health concerns, and socio-economic reasons, as well as long cooking procedures, adversely affect legume consumption frequency. Pre-treatment methods, including soaking, sprouting, and pulse electric field technology, are effective in reducing the alpha-oligosaccharides and other anti-nutritional factors, eventually lowering cooking time for legumes. Extrusion technology used for innovative development of legume-enriched products, including snacks, breakfast cereals and puffs, baking and pasta, represents a strategic way to promote legume consumption. Culinary skills such as legume salads, legume sprouts, stews, soups, hummus, and the development of homemade cake recipes using legume flour could represent effective ways to promote legume consumption. This review aims to highlight the nutritional and health effects associated with legume consumption, and strategies to improve their digestibility and nutritional profile. Additionally, proper educational and culinary approaches aimed to improve legumes intake are discussed.

Characterization of aerosols generated by high-power electronic nicotine delivery systems (ENDS): Influence of atomizer, temperature and PG:VG ratios.

The aerosol characteristics of electronic nicotine delivery systems (ENDS) are important parameters in predicting health outcomes since parameters such as aerosol particle size correlate strongly to aerosol delivery and deposition efficiency. However, many studies to date do not account for aerosol aging, which may affect the measurement of ultra-fine particles that typically coagulate or agglomerate during puff development. To reduce aerosol aging, we herein present a unique instrumentation method that combines a) positive pressure ENDS activation and sample collection, b) minimization of both sample tubing length and dilution factors, and c) a high-resolution, electrical low-pressure impactor. This novel approach was applied to systematically investigate the effects of coil design, coil temperature, and propylene glycol to vegetable glycerol ratios on aerosol characteristics including aerosol mass generation, aerosol count generation, and the mass and count size distributions for a high-powered ENDS. Aerosol count measurements revealed high concentrations of ultra-fine particles compared to fine and coarse particles at 200°C, while aerosol mass measurements showed an increase in the overall aerosol mass of fine and coarse particles with increases in temperature and decreases in propylene glycol content. These results provide a better understanding on how various ENDS design parameters affect aerosol characteristics and highlight the need for further research to identify the design parameters that most impact ultra-fine particle generation.

A Microfluidic Experiment on CO2 Injection for Enhanced Oil Recovery in a Shale Oil Reservoir with High Temperature and Pressure

In recent years, CO2 huff and puff has become one of the most important methods developed for unconventional shale oil reservoirs and has been widely used in all major shale oil fields. However, the microscopic mechanism of CO2 contacting with crude oil is complex, and the change law of the residual oil occurrence after CO2 injection is unclear. In this paper, a micro visualization fluid flow simulation experiment (microfluidic experiment) under high temperatures and high pressure of a shale reservoir was conducted to reveal the micro mechanism of CO2 and crude oil after contact at the microscale. This allows conclusion of more precise results than any experiment conducted in a room environment. Combined with gas–oil two-phase micro flow characteristics, the production mechanisms of crude oil by CO2 huff and puff at the pore scale are clarified, and the change characteristics of the remaining oil occurrence state after CO2 injection are quantified. The results show that CO2 mainly produces crude oil in macropores and microfractures in the injection stage of huff and puff, improves the mobility of crude oil through diffusion dissolution in the soaking stage, and that the driving of dissolved gas is dominant in depressurization production. The major micro-mechanisms for CO2 to improve shale oil are extraction and dissolution expansion, accompanied by a variety of secondary mechanisms, such as the miscibility effect, oil expansion, viscosity reduction and other contact effects, as well as the improvement of crude oil properties. The simulation results of huff and puff development show that soaking is an important stage to enhance oil recovery. With increasing soaking time or the soaking pressure, the recovery degree of crude oil will increase positively.

Study on the development options of tight sandstone oil reservoirs and their influencing factors

The research area of tight sandstone oil reservoirs was selected, a numerical model of the oil reservoir was developed, and a study of the development options and influencing factors was carried out to analyze the influence of different development methods, physical and engineering parameters on the development dynamics. Study shows that the two main factors limiting the efficient development of tight sandstone reservoirs are reservoir properties and formation energy. Fractured horizontal well injection huff and puff development can effectively improve reservoir physical properties and timely replenish formation energy, which is suitable for the development of such oil reservoirs. In dense sandstone reservoirs, its impact on production capacity is also relatively small when the permeability ratio is small. Due to both gravity and reservoir physical properties, the permeability ratio increases, the cumulative oil production of positive rhythm reservoirs decreases and that of reverse rhythm reservoirs increases, and the location of high-quality reservoirs in the upper part of producing wells is conducive to increasing the final recovery rate. A lower oil to water viscosity ratio can significantly increase the swept volume and improve development effect. Hydrophilic reservoirs can reduce the injection pressure and increase the spread range, effectively improving the problem of inability to inject, and improving reservoir hydrophilicity through surface activators can increase reservoir recovery. The water injection rate determines the recovery rate of formation energy. Generally, the faster the rate, the higher the cumulative oil production. Therefore, the rate of water injection should be increased as much as possible, taking into account construction conditions and economic evaluation. Additionally, the effect of water injection on the development effect is different at different stages, so the appropriate timing of water injection is very important to the water injection huff and puff development effect, and the use of early water injection in this research area is not conducive. Soaking can promote pressure and fluid redistribution and improve water injection huff and puff development effect, but soaking for a long time can lead to reservoir contamination and reduce crude oil production, so the preferred time for a soaking is about 20 days.

A dynamic model of CO2 diffusion coefficient in shale based on the whole process fitting

CO2 diffusion is of great importance for enhanced oil recovery (EOR), production prediction, and performance forecast of carbon capture and storage (CCS) in shale reservoirs. This work aims to determine the diffusion coefficient of the shale reservoir during CO2 huff and puff development. Firstly, combining pressure decay experiments and mathematical modeling studies, this paper developed a dynamic method to determine the diffusion coefficients of CO2 in cores under shale reservoir conditions. Based on Fick's law, MATLAB software was used to build the fitting chart under different diffusion coefficients. The diffusion coefficient was determined by matching experimental data. During the experiment, the reservoir's pore pressure and confining pressure were simulated, and a saturation-depletion-diffusion experiment method was established to simulate the natural reservoir conditions. The effects of different initial pressures on diffusion were compared in pressure decay experiments. The mathematical model of the diffusion coefficient of CO2 was established, and the fitting chart of diffusion coefficient considering time step was derived. The diffusion distance of different diffusion coefficients and different times was compared. The results show that the diffusion coefficient of CO2 in shale is between 5*10-8 ~ 5*10-7 cm2/s, and the diffusion rate is 0.28 cm/d under the condition of 20 MPa and 319.55 K. In addition, when the diffusion coefficient increases by one order of magnitude, the diffusion distance increases by 200%.

A modified embedded discrete-fracture model to study oil-water two-phase heat and mass transfer in the complex fracture network

The temperature of the fluids injected is usually cooler than the in-situ reservoir temperature in the fractured reservoir's development. It is essential to study thermal changes between matrix and fracture. This paper establishes a fully implicit solution model for the coupling of the oil-water two-phase temperature field and seepage field based on the embedded discrete fracture model (EDFM) method. We deduced the mass and heat transfer between the local matrix grid and the fracture grid by the boundary element method (BEM) instead of a simple linear hypothesis to improve the traditional EDFM method. The model can accurately calculate the seepage field and temperature field in the two-dimensional or three-dimensional problem. Compared with the analytical solution, the discrete fracture model (DFM) and the traditional EDFM method proved that the modified EDFM method could significantly improve the calculation accuracy(especially the temperature field). Furthermore, the modified EDFM method is applied to four examples: waterflooding, huff and puff development, heterogeneous and natural fractured reservoirs, and multilayer enhanced geothermal system (EGS), which verifies the applicability, robustness, and computational convenience, of the new method and illustrates the influence and necessity of considering the coupling effect of temperature field and seepage field on the development effect. It provides an efficient and accurate new method for the study of non-isothermal seepage in fractured reservoirs.

Cooperative Mechanism and Technology Parameters Optimization of Multi-thermal Fluid Huff & Puff Development in Heavy Oil Reservoir

The extra-heavy oil reservoirs in Central Asia have the characteristics of shallow burial, high crude oil viscosity, and strong reservoir heterogeneity. Conventional steam huff&puff have good initial development effects, but because energy cannot be replenished in time, steam huff&puff in the later stages huff&puff production declined fast and the effective production time is short. According to the current situation of oil reservoir development, a development technology combining multi-thermal fluid huff&puff and steam huff&puff is proposed. At the initial stage of production, steam is used to reduce viscosity, and in the later stage, N₂ in the multi-thermal fluid is used to pressurize, expand the steam chamber volume, and dissolved CO₂ to reduce viscosity. For single wells of different modes, using numerical simulation to design reasonable combined development modes and injection parameters, the research shows that multi-thermal fluid huff&puff after 5 cycles of steam huff&puff can achieve the best oil increase effect, and the best steam injection intensity 10t/m, gas injection intensity of 1500m3/m, 29 wells have been implemented in M heavy oil field in Central Asia. after use the multi-thermal fluid and superheated steam huff&puff coordinated development methods, the average well daily oil increment reached 0.6-4t/d, the water cut is reduced by 10-21%, the oil-steam ratio is increased by 0.4-0.6, realizing the efficient development of shallow buried extra-heavy oil reservoirs.

Real-Time Measurement of Electronic Cigarette Aerosol Size Distribution and Metals Content Analysis.

Electronic cigarette (e-cigarette) use is increasing worldwide and is highest among both daily and nondaily smokers. E-cigarettes are perceived as a healthier alternative to combustible tobacco products, but their health risk factors have not yet been established, and one of them is lack of data on aerosol size generated by e-cigarettes. We applied a real-time, high-resolution aerosol differential mobility spectrometer to monitor the evolution of aerosol size and concentration during puff development. Particles generated by e-cigarettes were immediately delivered for analysis with minimal dilution and therefore with minimal sample distortion, which is critically important given the highly dynamic aerosol/vapor mixture inherent to e-cigarette emissions. E-cigarette aerosols normally exhibit a bimodal particle size distribution: nanoparticles (11-25nm count median diameter) and submicron particles (96-175nm count median diameter). Each mode has comparable number concentrations (10(7)-10(8) particles/cm(3)). "Dry puff" tests conducted with no e-cigarette liquid (e-liquid) present in the e-cigarette tank demonstrated that under these conditions only nanoparticles were generated. Analysis of the bulk aerosol collected on the filter showed that e-cigarette emissions contained a variety of metals. E-cigarette aerosol size distribution is different from that of combustible tobacco smoke. E-cigarettes generate high concentrations of nanoparticles and their chemical content requires further investigation. Despite the small mass of nanoparticles, their toxicological impact could be significant. Toxic chemicals that are attached to the small nanoparticles may have greater adverse health effects than when attached to larger submicron particles. The e-cigarette aerosol size distribution is different from that of combustible tobacco smoke and typically exhibits a bimodal behavior with comparable number concentrations of nanoparticles and submicron particles. While vaping the e-cigarette, along with submicron particles the user is also inhaling nano-aerosol that consists of nanoparticles with attached chemicals that has not been fully investigated. The presence of high concentrations of nanoparticles requires nanotoxicological consideration in order to assess the potential health impact of e-cigarettes. The toxicological impact of inhaled nanoparticles could be significant, though not necessarily similar to the biomarkers typical of combustible tobacco smoke.

Methods Study on Gas Channeling of <i>In Situ</i> Combustion Development in Developed Heavy Oil Reservoir

Now in situ combustion became one of the ways for the developed heavy oil reservoirs to convert development pattern and improve recovery. After long time steam huff and puff development in heavy oil reservoir, it was obvious that there was a big change on the oil zones and reservoir properties and the reservoir heterogeneity. When it was converted to in situ combustion, the injection gas often break through along the high permeability layers in the fire drive wells that make the sweep area smaller and decrease the reservoir recovery. By analysis on the gas channeling of in situ combustion occurred in the heavy oil reservoirs, the methods was put forward to develop heavy oil reservoir using in situ combustion reasonably including well space infilling, controlling the gas injection rate, adding roam surfactant agent and using fire drive with horizontal well assisted etc. to prevent the condition of gas channeling occurring. It will greatly improve the success and adaptability of the in situ combustion used in vertical wells of the heavy oil reservoir by using these methods. It also will build on basis of the heavy oil reservoir converted to in situ combustion development.

感触よくきれいに仕上げるファンデーション用パフの開発

感触よくきれいに仕上げるファンデーション用パフの開発

Unusual Dvosophila virilis stress-puff at 20CD: cytological localization of a heat sensitive locus and some peculiarities of the heat shock response

Using a series of chromosome aberrations a heat shock (hs) inducible site of the Drosophila virilis heat shock puff 20CD has been cytologically localized in the distal 20C subdivision: the hs puff was not observable in chromosome 2 carrying the Df(2)ebTG-52 deficiency; the bands in the proximal 20D subdivision could not autonomously be activated by hs in aberrant chromosomes that have this deficiency or the In(2)ebPC-19 inversion. An electron microscopy (EM) analysis of the successive stages of puff development in the 20CD and 20F regions shows that the 20CD puff is intially formed from the right part of the 20C6-7 band. In the maximally developed puff, the neighboring bands of the 20C and 20D regions are only partially decondensed; they are seen as discrete compact chromatin clumps. Specific differences in the ultrastructural organization and in response to hs have been found between the D. virilis "unusual" 20CD and typical 20F hs puffs.

4233 The Development of Puffs and Slugs in Laminar-to-Turbulent Transition in Pipe Flow

Laminar-to-Turbulent transition of pipe flows occurs, for sufficiently high Reynolds numbers, in form of slugs. These form as intermittent disturbances in the entrance region of a pipe, grows in axial direction as they move downstream and join at some distance of the pipe inlet. In this way, the fully development of turbulent flow is formed in the far downstream region. This is shown in the paper and the merging of slugs from the entrance to the exit of pipe flows is quantified. A special flow test rig was developed and built for the described investigations and was employed for detailed investigations of laminar-to-turbulent transition studies. The paper also describes the triggered transition at relatively lower Reynolds numbers yielding the transition of puffs. They form by the introduced disturbances and developed afterwards into slugs with a higher front than back edge velocity. Thereafter the flow development occurs as found for slug flows.

Cytochrome oxidase in the human visual cortex: distribution in the developing and the adult brain.

Cytochrome-oxidase (CO) histochemistry has revealed important functional subdivisions, modules, and processing streams in the macaque visual cortex. The present study is aimed at analyzing the development and characteristics of CO patterns in the human visual cortex by means of histochemistry and immunohistochemistry. At 26 weeks of gestation, both the ventricular and subventricular zones had low levels of CO, while the cortical plate had moderate levels of CO. At birth, supragranular CO-rich zones (puffs) were not clearly organized, indicating that the development of puffs in the neonatal striate cortex lags behind that of the macaque monkey, whose puffs appear weeks before birth. Puffs were more clearly discernible in human cortex at postnatal day 24, and became well organized by the fourth postnatal month. Layer IVc alpha in the neonate exhibited a higher level of activity and amount of CO than the central portion of IVc beta, which contained a dense aggregate of small neurons. The base of IVc beta, however, was often as CO reactive as IVc alpha. In contrast, the majority of specimens available to us from the fourth postnatal month and from adults with no known neurological diseases had significantly greater CO reactivity in layer IVc beta than in IVc alpha. Layer VI was moderately reactive for CO throughout development. In V2, stripes with globular zones of high CO activity were sporadically present at birth, suggesting that their development may parallel or precede that of puffs in V1. These stripes with CO-rich globular zones became more prominent in the adult and radiated orthogonally from the V1/V2 border. They were not, however, clearly organized into alternating thick and thin stripes as they are in the squirrel monkey. Visual cortical areas beyond V2 exhibited high CO activity mainly in layers III and IV and moderate levels in VI, suggesting that sites associated with cortico-cortical pathways may be metabolically most active.

Cytogenetic analysis of region 2B3-4-2B11 of the X-chromosome of Drosophila melanogaster

About 160 kb of DNA were cloned from the 2B region of the X chromosome, where the early ecdysone puff develops and the ecs locus is located. On the physical map of this sequence the positions of 13 chromosome rearrangement breakpoints interfering with both puff development and the ecs locus proximally and distally, were plotted by means of in situ hybridization. The maximal size of the ecs locus is about 100 kb (between the breakpoint of In(1)Hw 49c and the proximal end of Df(1)St472) The DNA sequences essential for normal puffing are located within the ecs locus between the In(1)br lt103 and Df(1)St472 breakpoints and comprise about 65 kb. Thus the puff develops as a result of ecs activation. Since Df(1)P154, which reduces the puff size and removes the proximal part of the ecs locus, does not prevent puff induction by ecdysone, while removing the distal part of the locus by Df(1)St469 completely stops development of the puff, we conclude that the regulatory zone of the locus, which reacts to hormone is located in the distal parts of both the puff and the locus, proximal to the breakpoint of In(1)br lt103 .Since In(1)br lt103 , Df(1)pn7b and Df(1)br R1 damage ecs but do not prevent puffing it is proposed that there is a second regulatory zone for this locus with a minimal size of 15–20 kb (between the breakpoints of Df(1)br R1 and In(1)br lt103). After cytogenetic and electron microscopic analysis of 2B puff formation it seems very likely that the site of puff formation is situated in the proximal part of 2B3-4 and after enhancement of ecs expression by hormone it spreads proximally to the 2B6 band which does not puff. When the puff regresses at puff stages (PS)10-11 its material does not condense completely and a zone of residual puffing joins the condensed material located distal to it. This material can give the impression of a separate band, designated 2B5 in Bridges' map. For convenience we propose to call the site giving rise to the puff as 2B3-5.

Cytogenetic analysis of the X-chromosome region 2B1-2-2B9-10 of Drosophila melanogaster

In salivary glands of yellow control stock the puffing pattern in the ecdysone-added artificial C46P medium was on the whole similar to that observed during larval development in vivo. However, underdevelopment of a series of late puffs and a delay in the regression of early puffs were observed. In addition a set of “medium” puffs not visible in vivo appeared. Late puffs differed from those developing in Grace medium. When salivary glands of homozygotes for the lethal dor lt187, a mutation that causes death in the third instar with no signs of ecdysone induction were incubated with ecdysterone, the development of puffs was restored, i.e., the puffing pattern of mutant cells in vitro practically did not differ from that in cells of the control stock. This implies that the dor lt187 lethal allele belongs to the class of ecdysone-deficient mutations.

Environmental influences on puffing in the salivary gland chromosomes of Drosophila melanogaster.

SUMMARYThe sequence of development of salivary gland chromosome puffs inDrosophila melanogastercultured on live yeast and RNA-deficient medium has been studied in late larval and prepupal stages. Differences in puffing pattern between individuals cultured in the two environments are almost entirely due to shifts in the relative timing of puff development at specific chromosome sites. Detailed studies on the right arm of the second chromosome indicate that, at certain sites, puffing activity spreads to adjacent lettered subdivisions of the chromosome. There are differences in the extent of lateral spreading of activity into adjacent regions in the two environments which may be due to clustering of functionally related genes.

Hydroxyurea-induced inhibition of DNA puff development in the salivary gland chromosomes of Bradysia hygida.

The injection of hydroxyurea at a critical time during the fourth larval instar inhibits the development of all DNA puffs in the salivary gland chromosomes of Bradysia hygida. RNA puff formation is not disturbed and larval development continues. The effect is explained as a result of a selective and general inhibitory action of the drug on DNA synthesis during the time when gene amplification occurs in the salivary glands. The incorporation of uridine into the chromosome regions where DNA puff development has been inhibited is sharply decreased in comparison with the incorporation into non-amplifying parts of the same chromosomes. The interpretation proposed for the cytologic observations seems to offer a better understanding of the nature of the DNA puffs.

Factors involved in the expression of gene activity in polytene chromosomes.

In order to separate some of the factors involved in the formation of puffs the antibiotic actinomycin D was applied at different stages of puff activity. Puffs were induced by temperature shocks or eodysone. Inhibition of RNA synthesis with actinomycin D before application of a puff inducing stimulus prevents neither the appearance of the stimulus specific puffs nor the accumulation of acidic proteins in the puff regions. The puffs attained under these conditions approximately 1/3 of the size normally produced by the stimulus. Indications were obtained that during puff formation acidic protein accumulation precedes the onset of RNA synthesis. Synthesis and storage of newly synthesized RNA within the puff region was studied on the basis of grain distribution in uridine-H3 autoradiographs after various incubation periods. RNA synthesis appears to be restricted to a particular area of the puff region. After a 3 min temperature shock following injection of uridine-H3 silver grains are located only over a particular area of the newly formed puff. The same area becomes labeled during a 1 min pulse of uridine-H3 applied at a stage of maximum puff development. Longer periods of incubation result in a random distribution of the grains over the whole puff region. Grain counts on different areas of experimentally induced puffs and on the same areas at a stage of puff regression indicate that the newly synthesized RNA becomes transferred from the area where it was synthesized and is stored for a certain period within the puff region. Complete release of newly synthesized RNA from puffs in which RNA synthesis was inhibited by actinomycin D at a stage of maximal activity is accomplished within 30 to 35 min.

Tritiated‐histidine incorporation into Drosophila salivary chromosomes

AbstractAn autoradiographic study of H3‐histidine incorporation into nonhistone protein of explanted larval salivary gland chromosomes of D. virilis showed patterns of incorporation that were dependent upon the stage of larval development. The sequence of changes in the development of several puffs in a specific chromosomal region was followed using the appearance of pigment in the anterior spiracles as a means of larval staging. H3‐histidine incorporation into these puffs in prepupae occurred as the puffs were regressing in size and protein staining. Acid extraction of histone and nucleic acid failed to alter the character of the autographs; presumably a non‐histone protein is involved in the H3‐histidine incorporation. Other puff sites in the same prepupal chromosomes showed various patterns of isotopic amino acid incorporation indicating that the pattern reported for a specific region may not be true for all puff sites.

Puff development and DNA synthesis in Sciara salivary gland chromosomes in tissue culture.

AbstractA method for the tissue culture of salivary glands of Sciara coprophila in a chemically defined medium is presented. When the chromosomes of the posterior lobe of the gland have puffs and the chromosomes of the anterior lobe do not have puffs prior to culture, puffs will develop in the anterior lobe chromosomes within a 24 hour culture period. When these chromosome II puffs are at their largest, fully extended state and a bulb on chromosome III is in a larva nearing pupation, the puffs and bulb become condensed in culture more rapidly than in normal development. This condensed state of the chromatin is thought to be heterochromatin. The uptake of tritiated thymidine was compared in homologous glands of 20 larvae in six different stages of development and all glands were labeled before and after culture, indicating that the culture method does not interfere with DNA synthesis.