Goal. To determine the effectiveness of biological and chemical fungicides in the control of fungal pathogens on crops of different varieties of winter barley under irrigation. Methods. Field — to determine the features of fungal diseases of winter barley plants in the years of research; laboratory – to identify pathogens, calculate technical efficiency of fungicides; statistical — to assess the reliability of the obtained experimental data. Results. The most common fungal diseases of winter barley plants in irrigated lands in Southern Ukraine were reticular spot (Drechslera teres Shoem), rhinosporiosis (Rhynchosporium graminicola Heinsen), powdery mildew (Erysiphe graminis f. Speiformis), and yellow rust (Puccinia striiformis West). The application of biological preparations Pseudobacterin 2 (1.0 l/ha) in VVSN31 and Baktofit (2.5 l/ha) in VVSN49 on crops of the Academichnyi variety for the optimal sowing period (10.10) provided technical efficiency against pathogens 31.9–77,8%, and for the late sowing period (20.10) — 24.0–72.3%. In the variety Dostoinyi biofungicides provided slightly lower technical efficiency, which for the optimal sowing date was 31.5–77.8%, and for the late sowing date — 22.8–71.7%. The technical efficiency of the use of chemicals Soligor (0.8 l/ha) in VVSN31 and Adeksar Plus (1.0 l/ha) in VVSN49 against these fungal pathogens was higher and in the varieties Academichnyi and Dostoinyi for optimal and late sowing was 58,4–96.9 and 70.8–98.2%, and 54.2–95.5 and 63.4-94.8%, respectively. Conclusions. In the conditions of irrigation on winter barley varieties Academichnyi and Dostoinyi against diseases of fungal etiology complex application of biological fungicides Pseudobacterin 2 (1.0 l/ha) and Baktofit (2.5 l/ha) provide moderate and medium, and preparations of chemical nature Soligor (0,8 l/ha) and Adeksar Plus (1.0 l/ha) — medium and high technical efficiency. Regardless of the variety and sowing date, the technical efficiency of biological preparations against the pathogen Drechslera teres Shoem was in the range of 22.8–31.9%, Rhynchosporium graminicola Heinsen — 34.1–36.9%, Erysiphe graminis f. Speer — 71.7–77.8 and Puccinia striiformis West — 34.4–63.6%, and chemical fungicides — 62.1–70.8%, 54.2–76.4, 92.1–96, 9 and 75.0–98.2%, respectively.