Pteris Ensiformis Research Articles

Potential of Endophytic Bacteria in Controlling The Red Jabon Leaf Spot Pathogens In Vitro

Plant pathogens pose a significant challenge to the cultivation of red Jabon (Anthocephalus macrophyllus) seedlings. Notably, the fungi Rhizoctonia sp. and Pestalotia sp. are responsible for the destructive red leaf spot disease on Jabon leaves. To combat these fungal pathogens, the use of endophytic bacteria has emerged as a potential alternative. Endophytic bacteria can be isolated from various plant sources, although their effectiveness in controlling forest plant pathogens like Jabon has not been thoroughly explored. This study aimed to assess the potential of endophytic bacterial isolates obtained from Pteris ensiformis (Isolates APE15, APE22, APE33, and APE35) in controlling the growth of Rhizoctonia sp. and Pestalotia sp. through in vitro experiments. The antibiosis activity of the endophytic bacteria against the pathogenic fungi was evaluated using the dual culture method on PDA media. The results revealed that among the four endophytic bacterial isolates, APE35 exhibited the highest inhibitory effect on Rhizoctonia sp. (86.79%) and Pestalotia sp. (67.5%), while isolate APE22 only inhibited the growth of Pestalotia sp. (55%). In contrast, isolates APE15 and APE33 were unable to suppress either fungus. The antibiosis activity of APE35 and APE22 resulted in abnormal hyphal growth of the pathogenic fungi, characterized by shriveled, bent, dark-colored, and coiled hyphae. Physiological characterization of the endophytic bacteria revealed their ability to produce protease, cellulase, catalase, and phosphate-dissolving enzymes. Additionally, these bacteria exhibited a Gram-positive nature. This study provides valuable insights into the potential of endophytic bacterial isolates from P. ensiformis (APE35 and APE22) as biological control agents for Rhizoctonia sp. and Pestalotia sp., the causative agents of red Jabon leaf spot disease.

Accumulation, translocation, and fractionation of rare earth elements (REEs) in fern species of hyperaccumulators and non-hyperaccumulators growing in urban areas

The issue of ion-adsorption type rare earth deposits (IADs) in urban areas of South China has garnered significant attention due to its environmental implications. Hyperaccumulator-based phytoremediation is a potentially effective solution for reducing the environmental impact of IADs in urban areas, particularly using ferns as they are known to be REE hyperaccumulators. However, the ability of different fern species to accumulate REEs in urban areas remains unknown. In this study, four fern species, including known hyperaccumulators (Dicranopteris linearis and Blechnum orientale) and other ferns (Pteris ensiformis and Cibotium barometz), were studied to investigate their REE accumulation abilities in the Guangzhou urban area. The aboveground parts of Dicranopteris linearis (848.7 μg g−1) and Blechum orientale (1046.8 μg g−1) have been found to accumulate high concentrations of REEs, demonstrating they probably can be applied for phytoremediation in the natural environments. Despite having lower REE concentrations than REE hyperaccumulators, Pteris ensiformis and Cibotium barometz still probably have the function as phytostabilizers in urban areas, as REEs can be enriched in their roots beyond the normal levels of plants. The enrichment of REEs in ferns is influenced by the availability of various nutrients (K, Ca, Fe, and P), which probably can be associated with different growth processes. The four fern species show LREE enrichment, moderate Eu anomalies and different Ce anomalies. It is difficult to absorb and transfer Ce to the aboveground parts of Blechnum orientale and Cibotium barometz. The study also identified selective enrichment of Ce in Pteris ensiformis, which has potential for comprehensive extraction of REEs when combined with other REE hyperaccumulators. REE fractionations are probably determined by the specific characteristics of different fern parts. Overall, these findings provide insights for addressing potential environmental problems related to IADs and offer guidelines for phytoremediation technology in addressing high REE levels in urban areas.

Chemical constituents of Pteris ensiformis from Guizhou

The phytochemical investigation of Pteris ensiformis from Guizhou has resulted in the isolation of 15 phenolic compounds (1–15), including kaempferol-3-O-glucoside (1), kaempferol-3-O-rhamnoside (2), kaempferol-4′,7-dimethyl ether-3-O-glucoside (3), apigenin-7-O-glucopyranoside (4), diosmetin-7-O-glucoside (5), esculetin (6), 4-methoxycinnamic acid (7), (+)-pinoresinol (8), bockioside B (9), 7-O-caffeoyl-hydroxymaltol-3-O-glucopyranoside (10), cylindol B (11), salicylic acid (12), vanillin (13), vanillic acid (14) and catechol (15). The structures of these compounds were determined based on the analysis of spectroscopic data and comparison with the data reported in literatures. Compounds 1–9, 11–15 were isolated from P. ensiformis for the first time. In addition, the chemotaxonomic significance of the isolated compounds was discussed.

Comparative assessment of bacterial diversity and composition in arsenic hyperaccumulator, Pteris vittata L. and non-accumulator, Pteris ensiformis Burm

The use of arsenic (As) for various industrial and agricultural applications has led to worldwide environmental contamination. Phytoremediation using hyperaccumulators is a sustainable soil As mitigation strategy. Microbial processes play an important role in the tolerance and uptake of trace elements such as in plants. The rhizospheric and endophytic microbial communities are responsible for accelerating the mobility of trace elements around the roots and the production of plant growth-promoting compounds and enzymes. Several studies have reported that the As hyperaccumulator, Pteris vittata L. (PV) influences the microbial community in its rhizosphere and roots. Deciphering the differences in the microbiomes of hyperaccumulators and non-accumulators is crucial in understanding the mechanism of hyperaccumulation. We hypothesized that there are significant differences in the microbiome of roots, rhizospheric soil, and bulk soil between the hyperaccumulator PV and a non-accumulator of the same genus, Pteris ensiformis Burm. (PE), and that the differential recruitment of bacterial communities provides PV with an advantage in As contaminated soil. We compared root endophytic, rhizospheric, and bulk soil microbial communities between PV and PE species grown in As-contaminated soil in a greenhouse setting. There was a significant difference (p < 0.001) in the microbiome of the three compartments between the ferns. Differential abundance analysis showed 328 Amplicon Sequence Variants (ASVs) enriched in PV compared to 172 in PE. The bulk and rhizospheric soil of both ferns were abundant in As-resistant genera. However, As-tolerant root endophytic genera were present in PV but absent in PE. Our findings show that there is a difference between the bacterial composition of an As hyperaccumulator and a non-accumulator species grown in As-contaminated soil. These differences need to be further explored to develop strategies for improving the efficiency of metal uptake in plants growing in As polluted soil.

The exotic species Pteris ensiformis (Pteridaceae) in South America: an aproach to naturalization process

Abstract Pteris has numerous representatives of Asian and Oceanic origin naturalized in America, some of them recognized as invasive weeds. Pteris ensiformis was introduced in America in the middle of the 19th century and subsequently naturalized in USA and Central America, but the records of its distribution in South America are isolated and the establishment or naturalization of the populations is unclear. As part of a broader study whose objective is to review the diversity of ferns and lycophytes in the Paranaense forest P. ensiformis was found for the first time in Argentina. In addition to describing, illustrating, and distinguishing the species from other species of the genus in the country, we review the distribution of P. ensiformis in South America comparing herbarium collections and iNaturalist observations, a citizen science platform. Additionally, a status for the species is proposed or suggested in each country where it is registered according to its stage of the naturalization process.

Phytate and Arsenic Enhance Each Other's Uptake in As-hyperaccumulator Pteris vittata: Root Exudation of Phytate and Phytase, and Plant Uptake of Phytate-P.

Phytate as a root exudate is rare in plants as it mainly serves as a P storage in the seeds; however, As-hyperaccumulator Pteris vittata effectively secretes phytate and utilizes phytate-P, especially under As exposure. This study investigated the effects of As on its phytate and phytase exudation and the impacts of As and/or phytate on each other's uptake in P. vittata through two hydroponic experiments. Under 10-100 μM arsenate (AsV), the exudation of phytate and phytase by P. vittata was increased by 50-72% to 20.4-23.4 μmol h-1 g-1 and by 28-104% to 18.6-29.5 nmol h-1 plant-1, but they were undetected in non-hyperaccumulator Pteris ensiformis at 10 μM AsV. Furthermore, compared to 500 μM phytate, the phytate concentration in the growth media was reduced by 69% to 155 μM, whereas the P and As contents in P. vittata fronds and roots were enhanced by 68-134% and 44-81% to 2423-2954 and 82-407 mg kg-1 under 500 μM phytate plus 50 μM AsV. The increased P/As uptake in P. vittata was probably attributed to 3.0-4.5-fold increase in expressions of P transporters PvPht1;3-1;4. Besides, under As exposure, plant P may be converted to phytate in P. vittata roots, thereby increasing phytate's contents by 84% to 840 mg kg-1. Overall, our results suggest that As-induced phytate/phytase exudation and phytate-P uptake stimulate its growth and As hyperaccumulation by P. vittata.

Effects of kinetin on arsenic speciation and antioxidative enzymes in fronds of the arsenic hyperaccumulator Pteris cretica var. nervosa and non-hyperaccumulator Pteris ensiformis

Cytokinins (CTKs) enhance plant tolerance to heavy metals, but the associated mechanisms are not yet fully understood. Here, we investigated the effects of kinetin (KT, a type of CTKs) on arsenic (As) speciation and antioxidative enzymes in plant fronds, by comparing Pteris cretica var. nervosa (As hyperaccumulator) and Pteris ensiformis (non-hyperaccumulator). A hydroponic test was carried out and the two plant species were treated with 2 mg L−1 arsenite [As(III)], arsenate [As(V)] or dimethylarsinate (DMA), and then 0–1.0 mg L−1 KT was exogenously applied for 14 days. The maximum As uptake of the two experimental plants was 0.5 mg L−1 KT. Fresh plant biomass was also significantly increased, except in P. cretica var. nervosa under DMA stress. The frond superoxide dismutase (SOD) and catalase (CAT) activity in the two plant species was significantly increased by 0.5 mg L−1 KT. Additionally, a significant decline in frond peroxidase (POD) activity was observed in P. ensiformis at 1.0 mg L−1 KT under As(III) stress. However, POD activity was significantly increased in P. cretica var. nervosa, at 0.1 mg L−1 KT, under As(III) or DMA stress, and at 1.0 mg L−1 KT, under As(V) stress. Therefore, KT promoted As transport and increased frond POD activity in P. cretica var. nervosa, which contributed to its As tolerance under stress from different As species.

Antagonistic activity of volatile organic compounds of endophytic bacteria from sword brake fern (Pteris ensiformis) against soil-borne fungal pathogens

The use of endophytic bacteria in the control of plant diseases, especially soil pathogenic fungi such as showed good results. Endophytic bacteria have various mechanisms to control plant pathogen, but the mechanism of endophytic bacteria in producing volatile organic compounds (VOCs) has not been widely studied. The objective of this research was to evaluated the antagonistic activity of VOCs produced by endophytic bacteria from the nail sword (Pteris ensiformis) aginst three soil borne pathogen fungus, R. solani, S. rolfsii and Fusarium sp. Endophytic bacterial isolates used were APE15, APE22, APE33, and APE35. The study was conducted by cupping method to determine the inhibition of endophytic bacteria VOCs against three soil borne fungal pathogens. GC-MS analysis was conducted to determine the content of volatile compounds produced by endophytic bacteria. The results showed that four endophytic bacterial isolates can inhibited R. solani growth (92.11%-96.05%), S.rolfsii (87.28%-93.10%), and Fusarium sp. (27.73%-57.55%). The GC-MS analysis showed that there were several antifungal compounds detected i.e. octanal, cytronellyl acetate, silane, 9-octacenamide, n-dimetylpalmitamide, and isobutyryl chloride. Octanal was presumed as the most influential compound that inhibited the three soil borne fungal pathogens because it was a dominant compound was detected.

English

Experiments were conducted with arsenic-hyperaccumulator, Pteris vittata, and non-hyperaccumulator, Pteris ensiformis, subjected to various arsenic toxicity levels (0 to 1500 mg As kg-1) in peat-moss or soil for up to 14 weeks, to evaluate phenotypic responses and total lipid with fatty acid profiles, and P. vittata influence on rhizosphere enzyme activities. P. ensiformis exhibited significant phenotypic toxicity response compared to P. vittata. P. vittata sequestered significantly (p&lt;0.5) higher arsenic (5,160.6 mg As kg-1) than P. ensiformis (313.4 mg As kg-1). Cellular damage and physiological death occurred in P. ensiformis with alteration in fatty acids and lipid compositions but no significant changes in P. vittata. Both showed no detectable C16:3 but a decrease in C18:3, which was more pronounced in P. ensiformis (51.2%); hence, are reported as C18:3 plants for the first time. Acid phosphatase, &beta;-glucosidase, alrysulphatase, and N-acetyl-&beta;-D-glucosaminidase activities were inhibited by As and significantly (p&lt;0.5) negatively correlated to As concentration. Enzyme inhibition range from 6% in &beta;-glucosidase to 67% in N-acetyl-&beta;-D-glucosaminidase. These results revealed P. vittata phenotypic tolerance to As stress was mediated through metabolic-readjustment, especially its fatty acid and lipid compositions. Additionally, P. vittata rhizospheric events modulate soil enzyme activities in the As-contaminated soil. Key words: Fern, heavy metal, phenotypic response, fatty acids, acid phosphatase, &beta;-glucosidase, alrysulphatase, N-acetyl-&beta;-D-glucosaminidase, rhizosphere.

Ferns Vegetation in the Lemor Botanical Garden, Suela District, East Lombok Regency

Lemor Botanical Garden is located in Suela Village, East Lombok Regency, West Nusa Tenggara. Various plant exist in the Lemor Botanical Garden. One of them is ferns. Research on the identification of ferns in the Lemor Botanical Garden was carried out from July to September 2020. The purpose of this research was to determine the spesies of ferns in the Lemor Botanical Garden area. This research is a descriptive research. The data was collected by using the sruise method. The results showed that in The Lemor Botanical Garden there were 12 families and 29 spesies of ferns. The spesies of ferns found were: Tectaria angulata, Tectaria melanocaula, Tectaria palmate, Tectaria SP, Pteridrys syrmatica, Nephrolepis cordifolia, Nephrolepis falcate, Stenochlaena palustris, Drynaria quercifolia, Lemmaphyllum carnosum, Adiantum tenerum, Pteris vittata, Pteris ensiformis, Pteris biaurita, Pteris asperula, Cheilanthes tenuifolia, Antrophyum sessilifolium, Elaphoglossum norsii, Diplazium esculentum, Pityrogramma calomelanos, Macrothelypteris torresiana, Spaerostephanos polycarpus, Mesophlebion sp., Pneumatopteris truncate, Amphineuron immersum, Pneumatopteris sp., Microlepia speluncae, dan Asplenium subnormale.

Chemical constituents from Pteris ensiformis Burm. (Pteridaceae)

Phytochemical study of Pteris ensiformis Burm. resulted in the isolation of eleven compounds, including three sesquiterpenoids (1–3), one alkaloid (5), one amino acid (6), one flavonoid (7), four phenolic acids (4, 8, 9 and 10), and one coumarin (11). Structures of these compounds were deduced from the ESI-MS and NMR data. This is the first report on the isolation of compounds 1, 2, 7 and 11 from P. ensiformis, compounds 6 and 9 from the genus Pteris, and compound 5 from the family Pteridaceae. In addition, a detailed discussion of the chemotaxonomic significance of these compounds was presented.

The ferns and lycophytes of Reserva Natural Guaricica, Antonina, Paran&amp;aacute;, Brazil

We found 204 species of pteridophytes in Reserva Natural Guaricica, a private natural heritage reserve (RPPN) in Antonina, Paran&amp;aacute;, Brazil. With approximately 8,600 ha and elevations ranging from sea level to 600 m, RPPN Guaricica has more species of pteridophytes than any other area in Paran&amp;aacute;. Ferns are represented by 194 species in 82 genera and 26 families, whereas lycophytes comprise 10 species in four genera and two families. The RPPN is the type locality of two recently described species: Hypolepis acantha Schwartsb. and Oleandra australis Schwartsb. &amp;amp; J.Prado. It is also the only place of occurrence of Didymoglossum angustifrons F&amp;eacute;e, Diplazium riedelianum (Bong. ex Kuhn) C.Chr., Pteris ensiformis Burm.f., P. tripartita Sw., Saccoloma elegans Kaulf., and Steiropteris polypodioides (Raddi) Salino &amp;amp; T.E.Almeida in Paran&amp;aacute;. Pteris ensiformis and Saccoloma brasiliense (C.Presl) Mett. are new state records. Additional species are expected to occur in the area, in view of their known geographical ranges.

Bakteri Endofit dari Tumbuhan Paku-pakuan sebagai Agens Hayati Rhizoctonia solani dan Pemacu Pertumbuhan Tanaman Padi

Rhizoctonia solani merupakan salah satu patogen penting penyebab penyakit hawar seludang pada tanaman padi. Patogen ini termasuk yang sulit dikendalikan karena bersifat kosmopolit, mampu bertahan di dalam tanah, dan memiliki kisaran inang yang banyak. Pengendalian secara biologi dengan bakteri endofit merupakan salah satu alternatif yang potensial karena kemampuannya hidup di dalam jaringan tanaman tanpa menimbulkan gejala penyakit dan dapat meningkatkan pertumbuhan serta ketahanan tanaman. Tujuan penelitian ini ialah mengeksplorasi bakteri endofit dari bagian batang akar dari tiga jenis tumbuhan paku yang berbeda, yaitu Salvinia molesta (paku air), Pteris ensiformis (paku terestrial), dan Drymoglossum pilosolloides (paku epifit) dan mengevaluasi potensinya sebagai agens hayati R. solani dan pemacu pertumbuhan tanaman. Isolasi bakteri dilakukan pada medium tripsic soy agar (TSA) dan nutrient agar (NA). Isolat bakteri dimurnikan dan diuji keamanan hayati dengan uji hipersensitifitas dan hemolisis. Hasil penelitian diperoleh 178 isolat bakteri endofit dan sebanyak 88 isolat menunjukkan reaksi negatif pada uji hipersensitisfitas dan uji hemolisis. Selanjutnya, isolat tersebut diuji dual test secara in vitro terhadap R. solani dan didapatkan 4 isolat bakteri endofit (APE15, APE22, APE33, dan APE35) dengan daya hambat dari 27–76% pada medium TSA, agar-agar dekstrosa kentang (ADK), dan TSA + ADK. Hasil uji pertumbuhan empat isolat terhadap benih padi varietas Situ Bagendit menunjukkan bahwa bakteri endofit mampu meningkatkan persentase perkecambahan padi 4.9% – 48.8%, meningkat panjang akar padi 68.3%–95.4%, panjang pucuk 53.2%–87.3%, bobot segar 49.07%–90.65%, dan bobot kering 48.1%–87.3%. Hasil ini menunjukkan bahwa tumbuhan paku mengandung beberapa bakteri endofit yang dapat dikulturkan dan memiliki senyawa antimikrob yang efektif, serta dapat digunakan sebagai sumber agens hayati terhadap patogen R. solani dan pemacu pertumbuhan tanaman.

Performance Evaluation of Ferns for Cut Green and Landscape Purpose

Ferns can be used as ground covers, specimen plants and for group, background and border planting in landscape. They can also be used as fillers in bouquets and flower arrangements. Hence, the objective of the study was to evaluate the performance of different fern species and to identify the suitable species for commercial cultivation. Eleven species of ferns belonging to different genera viz., Adiantum tenerum, Asplenium nidus, Asplenium longissimum, Asplenium scolopendrium, Diaplazium acrostichoides, Nephrolepis biserrata ‘furcans’, Nephrolepis exaltata ‘chidisii’, Nephrolepis exaltata, Bostoniensis compacta, Nephrolepis cordifolia, Nephrolepis biserrata miniata and Pteris ensiformis were evaluated for growth pattern and suitability for landscape and commercial uses. Vegetative characters like eplant height and spread were highest in Asplenium nidus and a maximum number of leaves were observed in Adiantum tenerum. Based on growth pattern they were classified under tall, medium and dwarf groups Asplenium nidus and Nephrolepis biserrata miniata were grouped under the tall category. The species which come under medium category are Asplenium longissimum, Diaplazium acrostichoides, Nephrolepis biserrata ‘Furcans’, Nephrolepis exaltata ‘Chidisii’, Nephrolepis exaltata, Bostoniensis compacta, Nephrolepis cordifolia, Pteris ensiformis and Asplenium scolopendrium where as Adiantum tenerum comes under dwarf category. All species evaluated were found to be suitable for pot plants. Nephrolepis biserrata miniata, Nephrolepis biserrata furcans, Nephrolepis cordifolia, Asplenium nidus and Diaplazium acrostichoides can be recommended as houseplants. Nephrolepis biserrata-Miniata, Nephrolepis biserrata furcans and Nephrolepis cordifolia can be used as border plants in landscapes. Nephrolepis exaltata, Bostoniensis compacta, Asplenium longissimum and Pteris ensiformis were observed to be attractive in hanging baskets. Nephrolepis biserrata-Miniata, Nephrolepis exaltata chidisii, Nephrolepis exaltata, Bostoniensis compacta and Diaplazium acrostichoides are suitable for bouquets and flower arrangements.

Performance Evaluation of Ferns for Cut Green and Landscape Purpose

Ferns can be used as ground covers, specimen plants and for group, background and border planting in landscape. They can also be used as fillers in bouquets and flower arrangements. Hence, the objective of the study was to evaluate the performance of different fern species and to identify the suitable species for commercial cultivation. Eleven species of ferns belonging to different genera viz., Adiantum tenerum, Asplenium nidus, Asplenium longissimum, Asplenium scolopendrium, Diaplazium acrostichoides, Nephrolepis biserrata ‘furcans’, Nephrolepis exaltata ‘chidisii’, Nephrolepis exaltata, Bostoniensis compacta, Nephrolepis cordifolia, Nephrolepis biserrata miniata and Pteris ensiformis were evaluated for growth pattern and suitability for landscape and commercial uses. Vegetative characters like eplant height and spread were highest in Asplenium nidus and a maximum number of leaves were observed in Adiantum tenerum. Based on growth pattern they were classified under tall, medium and dwarf groups Asplenium nidus and Nephrolepis biserrata miniata were grouped under the tall category. The species which come under medium category are Asplenium longissimum, Diaplazium acrostichoides, Nephrolepis biserrata ‘Furcans’, Nephrolepis exaltata ‘Chidisii’, Nephrolepis exaltata, Bostoniensis compacta, Nephrolepis cordifolia, Pteris ensiformis and Asplenium scolopendrium where as Adiantum tenerum comes under dwarf category. All species evaluated were found to be suitable for pot plants. Nephrolepis biserrata miniata, Nephrolepis biserrata furcans, Nephrolepis cordifolia, Asplenium nidus and Diaplazium acrostichoides can be recommended as houseplants. Nephrolepis biserrata-Miniata, Nephrolepis biserrata furcans and Nephrolepis cordifolia can be used as border plants in landscapes. Nephrolepis exaltata, Bostoniensis compacta, Asplenium longissimum and Pteris ensiformis were observed to be attractive in hanging baskets. Nephrolepis biserrata-Miniata, Nephrolepis exaltata chidisii, Nephrolepis exaltata, Bostoniensis compacta and Diaplazium acrostichoides are suitable for bouquets and flower arrangements.

Efficient enrichment of total flavonoids from Pteris ensiformis Burm. extracts by macroporous adsorption resins and in vitro evaluation of antioxidant and antiproliferative activities

The aim of this work is to develop an efficient and economical method for the enrichment of total flavonoids from Pteris ensiformis Burm. extracts. Resin screening, adsorption kinetics, adsorption isotherms and thermodynamics were successively researched prior to the dynamic adsorption and desorption tests. NKA-II resin was chosen as the best adsorbent, and the adsorption data were best described by the pseudo-second-order kinetics model and Langmuir isotherm model. The optimum enrichment conditions were as follows: for adsorption the total flavonoids concentration, flow rate and volume of sample were 1.84 mg/mL, 2 BV/h and 5 BV, respectively, and for desorption the flavonoids-loaded NKA-II resin column was desorbed by 7 BV of 50% ethanol at a rate of 2 BV/h. The product had a 6.63-fold higher total flavonoids content than crude extracts, and the recovery yield of total flavonoids was 80.65%. Furthermore, flavonoids-enriched extracts exhibited higher in vitro scavenging activity against superoxide anion radical and hydroxyl radical than crude extracts. In addition, higher antiproliferative activity of flavonoids-enriched extracts against MCF-7 and HepG-2 cell lines was also found as compared to the crude extracts. The developed method is appropriate for large-scale enrichment of total flavonoids from Pteris ensiformis Burm. extracts in the food and pharmaceutical industries.

Identifikasi Tumbuhan Paku di Hutan Penggaron Kecamatan Ungaran Kabupaten Semarang

&lt;p&gt;&lt;em&gt;Indonesia has a strategic geographical area with high biodiversity potential, it is located in the equatorial region which causes Indonesia have a tropical climate. The biodiversity of forests in Indonesia includes ferns. Nail plants can be found with a variety of types in various environments, especially in shady areas such as forests. Ferns are plants that have an important role in the forest ecosystem as a source of germplasm. Besides that, ferns also have the potential as a source of food and medicine for the Penggaron Forest, or who is familiarly called the Penggaron Tourism Area, is one of the natural tourism objects located in Semarang Regency, precisely in Susukan Village, Ungaran District, Semarang Regency. This study aims to identify the species of ferns in the Penggaron Forest. Data collection techniques used in identifying plant ferns are: 1) orientation and exploration of locations; 2) gathering ferns by exploring along the main road to the location of the campsite; 3) record and document the ferns found in the field. The results of the identification of ferns in the Penggaron Forest there are 2 families of ferns consisting of 23 species of plants. The Scizaeaceae family consists of 4 types of plants namely Lygodium flexuosum, Lygodium japonicum, Lygodium palmatum, and Lygodium circinatum. While the Polypodiaceae family consists of 19 types namely Nephrolepis hirsutula, Nephrolepis biserrata, Dryopteris scotii, Adiantum, Adiantum raddianum, Mickelopteris cordata, Pteris ensiformis, Pteris asperula, Pteris biaurita, Pteris vittata, Adiantum, Adiantum raddianum, Mickelopteris cordata, Pteris ensiformis, Pteris asperula, Pteris biaurita, Pteris vittata, Adiantum, Adiantum raddianum, Mickelopteris cordata, Pteris ensiformis, Pteris asperula, Pteris biaurita, Pteris vittata crenata, Tectaria angulata, Tectaria maingayi, Tectaria heracleifolia, Pleocnemia irregularis.&lt;/em&gt;&lt;/p&gt;

Potential of indigenous plant species for phytoremediation of metal(loid)-contaminated soil in the Baoshan mining area, China.

A field survey was conducted to investigate metal(loid) concentration in soils and native plants in the Baoshan mining area for potential application in phytoremediation. Total concentrations of arsenic (As), cadmium (Cd), lead (Pb), and zinc (Zn) in soil varied from 125 to 6656, 5.10 to 1061, 568 to 49294, and 241 to 17296 mg kg-1, respectively, showing severe contamination. Among 20 species native to this area, Pteris ensiformis accumulated 1091 mg kg-1 As in the shoot, and its translocation factor (TF) was greater than 1, suggesting potential capacity for As phytoextraction. Boehmeria nivea, Aster prorerus, and Hydrocotyle sibthorpioides showed potential for phytoextraction of Cd due to their high accumulation of Cd in shoots (490.3, 175.4, and 128.5 mg kg-1, respectively) and high TFs (92.0, 22.1, and 6.7, respectively). Eleusine indica and P. ensiformis were found to contain high concentrations of Pb (7474 mg kg-1) and Zn (1662 mg kg-1) in roots, but with low TFs for Pb (0.4) and Zn (0.2), suggesting potential capability for phytostabilization. There was a positive correlation (p < 0.01, N = 25) of TFs between the metal(loid)s, indicating a synergic interaction in the uptake of metal(loid)s by these plants. According to metal(loid) concentrations in shoots, bioconcentration factors (BFs), and TFs, as well as the botanical features such as wide occurrence, high biomass yield, and rapid growth of the plants, the five native species identified above have the potential for phytoremediation in the Baoshan mining area.

Typification and nomenclature of the ferns described in N.L. Burman's Flora Indica

Mazumdar, J., M.W. Callmander & N. Fumeaux (2019). Typification and nomenclature of the ferns described in N.L. Burman's Flora Indica. Candollea 74: 93-110. In English, English abstract. DOI: http://dx.doi.org/10.15553/c2019v741a10Nicolaas Laurens Burman (1734 – 1793) treated in his Flora Indica 69 pteridophyte names. Most of those names were described earlier by Carl Linnaeus (1707 – 1778) who was a friend of his father Johannes Burman (1706 – 1779). The Flora Indica (1768) is one of those early works that followed Linnaeus' binomial nomenclature formalized in 1753. N.L. Burman described 25 new ferns in this work. His herbarium is now stored as a separate historical collection at Geneva with the acronym G-PREL. We located all original material in G-PREL, with the goal to review the taxonomic identity and nomenclature of those 25 names. We also studied the Paul Hermann herbarium from Ceylon, bound in a folio volume now kept in the library of the Institut de France in Paris with the acronym BIF-CEYL, which was the main base of Johannes Burman's Thesaurus Zeylanicus (1737). Lectotypes are designated for twelve names lacking proper typification: Adiantum chinense Burm. f., Adiantum truncatum Burm. f., Ophioglossum pedatum Burm. f., Polypodium acutum Burm. f., Polypodium adianthoides Burm. f., Polypodium palustre Burm. f., Polypodium radicans Burm. f., Polypodium rostratum Burm. f., Polypodium simplex Burm. f., Polypodium trapezoides Burm. f., Pteris ensiformis Burm. f., and Trichomanes tenuifolium Burm. f.Received: August 19, 2018. Accepted: April 18, 2019. First published online: May 29, 2019.

Effect of arsenate on endogenous levels of cytokinins with different existing forms in two Pteris species

Our previous results showed that content of trans-zeatin (tZ) increases in leaves of heavy metal hyperaccumulators but decreases in non-hyperaccumulators growing in multiple heavy metal polluted soils. However, the relationship between arsenic (As) accumulation and endogenous forms of cytokinins (CTKs) in As hyperaccumulators remains unknown. Here a hydroponic experiment was conducted to compare the CTK forms in the As hyperaccumulator Pteris cretica var. nervosa and non-hyperaccumulator Pteris ensiformis under arsenate stress (0, 2, 5, and 10 mg L−1). A simple and cost-effective procedure for the determination of CTK forms in plants was established, and a stepwise regression analysis was used to study the relationship among total As contents and different forms of endogenous CTKs in fronds of two plants. The results showed that the optimized chromatographic parameters were Zobax SB-C18 column (5 μm × 4.6 mm × 250 mm), UV detection detector at 269 nm, a flow rate of 0.6 mL min−1, constant temperature of 45 °C and gradient elution with methanol-acetonitrile-1% acetic acid. Contents of chlorophylls in the fronds of P. ensiformis were significantly decreased with addition of As compared to P. cretica var. nervosa. Furthermore, the total As content in fronds of P. cretica var. nervosa was positively correlated to the contents of N6-(2-isopentenyl) adenine-7-β-D-glucoside (iP7G) and N6-(2-isopentenyl) adenosine (iPR). However, the total As content in fronds of P. ensiformis was negatively correlated to its trans-zeatin riboside (ZR) content. Therefore, iP7G and iPR could positively improve As accumulation by P. cretica var. nervosa.