Psychiatric Pharmaceuticals Research Articles

Tracking the surge of psychiatric pharmaceuticals in urban rivers of Curitiba amidst and beyond the SARS-CoV-2 pandemic

The heightened contamination of urban rivers with psychiatric drugs poses significant environmental and public health risks due to their persistence and bioaccumulative nature. In this study, we assessed the concentrations of 10 psychiatric medicines in water and sediment samples from three rivers in Curitiba, Brazil, spanning the SARS-CoV-2 pandemic from September 2020 to November 2023. Our analysis revealed substantial variations in drug concentrations across rivers and sampling periods, with fluoxetine and sertraline being the most prevalent in waters (up to 1118 ng L−1) and sediments (up to 70 ng g−1 DW). Sediments emerged as primary repositories for psychiatric drugs. The COVID-19 pandemic notably impacted drug concentrations, with fluoxetine and alprazolam concentrations surging by up to 741 % and 524 %, respectively, compared to pre-pandemic levels. The current concentrations of drugs in rivers pose risks to aquatic life. Despite variations, overall drug concentrations increased by 20 % for fluoxetine and 15 % for alprazolam after the pandemic. These findings underscore the persistent environmental risks associated with heightened drug consumption, emphasizing the urgent need for continued monitoring and intervention strategies to mitigate ecological and public health impacts.

In-stream attenuation and enantioselective fractionation of psychiatric pharmaceuticals in a wastewater effluent-dominated river basin

Wastewater effluent is the main contributor of psychiatric pharmaceuticals (PPs) pollution in surface waters. However, little is known about its spatial evolution dynamics in effluent-dominated rivers. Herein, 10 representative PPs, including 6 chiral pharmaceuticals and 4 achiral pharmaceuticals, were explored in the Beiyun River, a typical wastewater effluent-dominated river, to explore their occurrence, in-stream attenuation and enantioselective fractionation behaviors at a watershed scale. Among the target substances, 8 and 9 drugs were detected in surface water and sediment samples with the ΣPPs concentrations ranging from 78.4 to 260.1 ng/L and 4.8 to 43.4 ng/g dw in surface water and sediments, respectively. Along the mainstream of the Beiyun River, only several PPs detected in surface water, e.g., citalopram, O-demethylvenlafaxine, and fluoxetine, exhibited in-stream attenuation behaviors when reaching rural area, while all PPs detected in sediments displayed in-stream attenuation behavior. Four chiral PPs detected in surface water exhibited an enantioselective attenuation phenomenon, while in sediments, only citalopram displayed an enantioselective fractionation behavior. The differences in the in-stream attenuation and enantioselective environmental behavior of individual PPs caused complex contaminant evolution along the stream reach. This work provides enantiomeric profiles of chiral pollutants for evaluating their in-stream attenuation processes, which would facilitate better understanding of the changing contaminant exposure conditions in complex natural environments.

Photocatalytic Degradation of Psychiatric Pharmaceuticals in Hospital WWTP Secondary Effluents Using g-C3N4 and g-C3N4/MoS2 Catalysts in Laboratory-Scale Pilot

Today, the pollution caused by a multitude of pharmaceuticals used by humans has been recognized as a major environmental problem. The objective of this study was to evaluate and compare the photocatalytic degradation of ten target psychiatric drugs in hospital wastewater effluents using g-C3N4 and 1%MoS2/g-C3N4 (1MSCN) as photocatalytic materials. The experiments were performed using real wastewater samples collected from hospital wastewater treatment plant (WWTP) secondary effluent in spiked and inherent pharmaceutical concentration levels. The photocatalytic experiments were performed in a laboratory-scale pilot plant composed of a stainless-steel lamp reactor (46 L) equipped with ten UVA lamps and quartz filters connected in series with a polypropylene recirculation tank (55–100 L). In addition, experiments were carried out in a solar simulator apparatus Atlas Suntest XLS+ at a 500 Wm−2 irradiation intensity. The analysis of the samples was accomplished by solid-phase extraction, followed by liquid chromatography-Orbitrap high-resolution mass spectrometry. Results showed that the photocatalytic degradation of pharmaceutical compounds followed first-order kinetics. In all cases, 1MSCN presented higher photocatalytic performance than g-C3N4. The removal rates of the pharmaceutical compounds were determined above 30% and 54% using g-C3N4 and 1MSCN, respectively. Parallel to kinetic studies, the transformation products (TPs) generated during the treatment were investigated.

Occurrence and removal of psychiatric pharmaceuticals in the Tehran South Municipal Wastewater Treatment Plant.

Psychiatric drugs released by humans in wastewater have received more attention because of their potential risks for aquatic organisms. In this study, the occurrence of the two most common groups of psychiatric drugs (sedatives-hypnotics-anxiolytics and antidepressants) were evaluated in the Tehran South Municipal Wastewater Treatment Plant. All the target sedatives-hypnotics-anxiolytics (alprazolam, phenobarbital, and thioridazine) and antidepressants (fluoxetine, citalopram, sertraline, and venlafaxine) were observed in influent and secondary clarification (SC) effluent. Thioridazine (164.25 ± 218.74ng/L) and citalopram (672.53 ± 938.56ng/L) had the highest mean concentrations in the influent, while alprazolam (5.09 ± 2.33ng/L) and citalopram (776.97 ± 1088.01ng/L) had the highest concentrations in the SC effluent. The higher concentrations of the psychiatric drugs, except thioridazine, were detected in the SC effluent compared to the concentrations in the influent. The increased drugs concentrations, with negative removal efficiencies, were more distinctive in the cold season samples. Psychiatric drugs processed in the chlorination unit followed a completely different pattern compared to the drugs in the biological treatment unit. All the drugs' concentrations, except thioridazine, decreased in the chlorination unit, ranging between 27 ± 14% for alprazolam and 75 ± 10% for citalopram. However, the mean concentrations of the detected drugs were as follows: sertraline (11.96 ± 11.62ng/L) and venlafaxine (184.94 ± 219.74ng/L) which could cause environmental and ecological concerns.

Development and Validation of a Single Step GC/MS Method for the Determination of 41 Drugs and Drugs of Abuse in Postmortem Blood

A toxicology laboratory often receives a high number of samples from cases (autopsies or clinical) that may require the quick delivery of trustworthy, accurate results. Thus, there is a great need for a fast and reliable method that is capable of identifying and determining a large number of drugs and drugs of abuse in biological matrices, and especially in blood. In the present study, we describe the development of a fast and simple gas chromatography–mass spectrometry (GC-MS) method for the determination of 41 drugs and drugs of abuse (DOA) in blood. Sample pre-treatment by alkaline liquid–liquid extraction (LLE) was studied through the utilization of different solvents and solvent-to-sample ratios (v/v), which aimed to achieve a greater extraction efficiency and detection sensitivity with a decreased need for large sample volumes. Butyl acetate with a sample-to-solvent ratio of 4:1 (1 mL blood: 0.25 mL butyl acetate) was the most efficient. The method was validated for all analytes, and the evaluation parameters were within the acceptance criteria. The coefficient of determination (R2) was between 0.9934 and 1, the limits of detection (LODs) ranged between 1 ng/mL and 113 ng/mL, and the limits of quantification (LOQs) were between 4 ng/mL and 375 ng/mL for all analytes. The determinations were accurate (accuracy% from 84% to 114%) and precise (RSD% from 0.66% to 14.8% for low concentrations). Deconvolution Reporting Software (DRS) for GC-MS was optimized and applied for data analysis to enhance the identification potential, thereby avoiding false identifications (false positives) and increased productivity. The NIST Automated Mass Spectral Deconvolution and Identification Software (AMDIS) and the analytical utility Retention Time Lock (RTL) Database Library assisted in data evaluation. The method was applied to 89 postmortem cases (history of mental disorders and use of psychiatric pharmaceuticals) in which diazepam (0.13 to 4.34 μg/mL), citalopram (0.04 to 0.24 μg/mL), alprazolam (0.01 to 0.12 μg/mL), olanzapine (0.009 to 0.083 μg/mL), mirtazapine (0.01 to 0.33 μg/mL), venlafaxine (0.006 to 0.92 μg/mL), haloperidol (0.007 to 0.13 μg/mL), and zolpidem (0.01 to 0.16 μg/mL) were successfully quantitated.

Fate of selected pharmaceuticals in hospital and municipal wastewater effluent: occurrence, removal, and environmental risk assessment.

The concentrations and distribution of β-blockers, lipid regulators, and psychiatric and cancer drugs in the influent and effluent of the municipal wastewater treatment plant (WWTP) and the effluent of 16 hospitals that discharge into the wastewater treatment plant mentioned in this study at two sampling dates in summer and winter were examined. The pharmaceutical contribution of hospitals to municipal wastewater was determined. The removal of target pharmaceuticals was evaluated in a WWTP consisting of conventional biological treatment using activated sludge. Additionally, the potential environmental risk for the aquatic receiving environments (salt lake) was assessed. Beta-blockers and psychiatric drugs were detected in high concentrations in the wastewater samples. Atenolol (919 ng/L) from β-blockers and carbamazepine (7008 ng/L) from psychiatric pharmaceuticals were detected at the highest concentrations in hospital wastewater. The total pharmaceutical concentration determined at the WWTP influent and effluent was between 335 and 737 ng/L in summer and between 174 and 226 ng/L in winter. The concentrations detected in hospital effluents are higher than the concentrations detected in WWTP. The total pharmaceutical contributions from hospitals to the WWTP in summer and winter were determined to be 2% and 4%, respectively. Total pharmaceutical removal in the WWTP ranged from 23 to 54%. According to the risk ratios, atenolol could pose a high risk (risk quotient > 10) for fish in summer and winter. There are different reasons for the increase in pharmaceutical consumption in recent years. One of these reasons is the COVID-19 pandemic, which has been going on for 2 years. In particular, hospitals were operated at full capacity during the pandemic, and the occurrence and concentration of pharmaceuticals used for the therapy of COVID-19 patients has increased in hospital effluent. Pandemic conditions have increased the tendency of people to use psychiatric drugs. It is thought that beta-blocker consumption has increased due to cardiovascular diseases caused by COVID-19. Therefore, the environmental risk of pharmaceuticals for aquatic organisms in hospital effluent should be monitored and evaluated.Supplementary InformationThe online version contains supplementary material available at 10.1007/s11356-022-21131-y.

Simple and effective removal of psychiatric pharmaceuticals from wastewater treatment plant effluents by magnetite red mud nanoparticles

Psychological disorders due to the COVID-19 pandemic have increased the consumption of psychiatric pharmaceuticals on a global scale in last year. These compounds reach wastewater treatment plants (WWTP) through sewerage system and are not sufficiently removed by using conventional treatment process. Psychiatric drugs released with WWTP effluent may cause possible risks to the receiving aquatic environment. Also, two antidepressants have been included in the “watch list” in 2020. Therefore, simple and relatively cost effective removal of psychiatric drugs from wastewaters becomes important. Magnetite red mud nanoparticles (RM-NPs) synthesized was applied for the removal of psychiatric drugs (fluoxetine, paroxetine, carbamazepine, diazepam, and lorazepam) in WWTP effluent first time in this study. The adsorption of carbamazepine as a model compound was fitted well with pseudo-second-order kinetic model. The Freundlich isotherm model better represented the sorption data than the Langmuir model. High adsorption capacity (90.5 mg/g) was reached at 30 min contact time at pH 6.5–7.0 at 25 °C. The adsorption rate was described by the pseudo second order model and its rate control mechanism was controlled by film diffusion. The magnetite RM-NPs were efficiently used for the removal of carbamazepine from real WWTP effluents. The adsorption capacity and the magnetic separability of the regenerated magnetite RM-NPs were unaffected five cycles. Due to its simple application, low cost and high adsorption capacity, magnetite RM-NPs can be recommended as a better adsorbent comparing to commercial adsorbents to remove psychiatric drugs from WWTP effluents.

Occurrence and Fate of Psychiatric Pharmaceuticals in Wastewater Treatment Plants in Hong Kong: Enantiomeric Profiling and Preliminary Risk Assessment

Psychiatric pharmaceuticals are receiving widespread attention as emerging contaminants. Wastewater treatment plants (WWTPs) represent primary point sources for pharmaceuticals in the aquatic envir...

Drug pollution and pharmacotherapy in psychiatry: A "platypus" in the room.

Preoccupation about potential deleterious effects of pharmaceuticals in the environment is growing fast. Psychiatric pharmaceuticals have received particular attention because of their increasing use and their potential impacts on many living beings due to their effects on phylogenetically highly conserved neuroendocrine systems. Recent studies that have shown that many pharmaceuticals (including psychotropics) bioaccumulate through the web food have raised this concern into new heights. As professionals working in the field of psychiatry and academia, we believe we are about to enter a new era with regard to pharmacotherapy. We estimate drug pollution will have a major impact on our daily practice in a way we are just starting to imagine. So far, this problem has largely been ignored by healthcare professionals, who are the ones prescribing and dispensing pharmaceuticals. We are convinced that increasing awareness among these professionals will be a key element to effectively fight against drug pollution.

Development and Application of the Diffusive Gradients in Thin-Films Technique for Measuring Psychiatric Pharmaceuticals in Natural Waters

Psychiatric pharmaceuticals are widely distributed in the aquatic environment and have attracted recent attention due to their potential for environmental effects. A robust and reliable in situ passive sampling approach, the diffusive gradients in thin-films (DGT) technique, is developed here to measure 14 psychiatric pharmaceuticals. A new binding material, mixed-mode cation exchange resin (Poly-Sery MCX, 40 μm, CNW, Germany), was used for the first time in DGT and compared to XAD and HLB. Reliable elution efficiencies of the pharmaceuticals from the binding gels were obtained in methanol/ammonia, and diffusion coefficients for all the compounds were determined. The influence of diffusive layer thickness (0.515-2.015 mm), deployment time (3-168 h), and important environmental conditions-pH (3.02-9.45), ionic strength (0.0001-0.5 M), and dissolved organic matter (0-20 mg L-1)-were evaluated. The capacity of XAD, HLB, and MCX gels for binding all the test pharmaceuticals was ∼335 μg per disc, meaning that DGT could theoretically be deployed for over 30 months if there are no competitive effects or confounding factors. The uptake kinetics of psychiatric pharmaceuticals onto MCX gel were much faster than those onto XAD and HLB gels in the first hour. DGT measured concentrations of test pharmaceuticals at two sample points in a river (over 6 days) were comparable to those obtained by grab sampling. This study demonstrates the accuracy and reliability of DGT for measuring psychiatric pharmaceuticals across a wide range of freshwater conditions found in the natural environment.

Interactive Effects of Sertraline and Diphenhydramine on Biochemical and Behavioral Responses in Crucian Carp (Carassius auratus).

The ecotoxicity of psychiatric pharmaceuticals to aquatic organisms is being increasingly recognized. However, current ecological studies focus on the effects of individual psychiatric pharmaceuticals, with little attention being given to their combined effects. In this study, the interactive effects of two psychiatric pharmaceuticals, sertraline (SER) and diphenhydramine (DPH), on bioconcentration and biochemical and behavioral responses were investigated in crucian carp (Carassius auratus) after seven days of exposure. DPH was found to increase the accumulation of SER in fish tissues relative to SER-alone exposure. In addition, the mixture of SER and DPH significantly changed the activities of antioxidant enzymes and led to significant increases in malondialdehyde content, relative to SER alone. Concerning the neurotoxicity, relative to SER-alone exposure, brain AChE activity was significantly enhanced in fish following the combined exposure. Regarding behavioral responses, swimming activity and shoaling behavior were significantly altered in co-exposure treatments compared with the SER alone. Moreover, the inhibition effects on the feeding rates were increased in co-exposure treatments compared to SER alone. Collectively, our results suggest that the mixtures of psychiatric pharmaceuticals may pose more severe ecological risks to aquatic organisms compared to these compounds individually.

Psychoactive substances in mussels: Analysis and occurrence assessment

This work presents an analytical methodology based on a “Quick, easy, cheap, effective, rugged, and safe” (QuEChERS) extraction and liquid chromatography-tandem mass spectrometry detection (LC-MS/MS) for the simultaneous determination of 35 psychoactive substances in fresh mussel samples. The compounds investigated include illicit drugs, new psychoactive substances, commonly prescribed psychiatric pharmaceuticals, and caffeine. The methodology was validated in terms of recovery (relative recoveries 77–118%), repeatability (relative standard deviation values <20%), and sensitivity (limits of detection and quantification <2 ng/g fresh weight (f.w.) and <6.7 ng/g f.w., respectively, for most compounds). The method was applied to the analysis of 15 samples, covering both commercially available mussels purchased from local food markets and wild fresh mussels collected in the Northeast coast of Spain in the Mediterranean Sea. Only one sample corresponding to wild mussels was found to contain 2 of the target analytes, namely, sertraline (1.5 ng/g f.w.) and caffeine, (12.8 ng/g f.w.).

Combined effects of NaCl and fluoxetine on the freshwater planarian, Schmidtea mediterranea (Platyhelminthes: Dugesiidae).

Increasing salinity levels in freshwaters due to natural and anthropogenic sources pose risk to exposed aquatic organisms. However, there is a paucity of information on how salinity may influence the effects of other chemical stressors especially psychiatric pharmaceuticals. Freshwater planarians which have been suggested as bioindicator species in aquatic habitats were used in this study to evaluate toxic effects of sodium chloride (NaCl) used here as a surrogate for increasing salinity, and its influence on the effects of the antidepressant, fluoxetine. Effects of NaCl on Schmidtea mediterranea were evaluated using survival, regeneration, locomotion, feeding, and reproduction as endpoints. Subsequently, combined effects of NaCl and fluoxetine on planarians' locomotion and reproduction were also evaluated. Result showed that exposure to increased NaCl concentrations is toxic to planarians with 48 and 96h LC50 of 9.15 and 7.55g NaCl L-1 respectively and exposure to sub-lethal concentrations led to reductions in feeding (LOEC of 0.75g NaCl L-1 or 1906μScm-1 at 20°C) and reproduction (LOEC 3.0g NaCl L-1 or 5530μScm-1 at 20°C), delayed head regeneration (LOEC of 1.5g NaCl L-1 or 3210μScm-1 at 20°C), and also slight decreases in locomotor activity. Moreover, some developmental malformations were observed in regenerating planarians, as well as delayed or inhibition of wound healing and degeneration after fissioning and during head regeneration. A significant interaction between fluoxetine and NaCl was observed for locomotor activity and unlike planarians exposed to fluoxetine alone, fissioned planarians and their pieces from the combined exposure treatments were also unable to regenerate missing portions. Results show that S. mediterranea can be highly sensitive to low NaCl concentrations and that this stressor can alter the effects of fluoxetine. The implication of these effects for planarian populations in the natural habitat is discussed as well as the need for more research on the effects of neuroactive pharmaceuticals under relevant exposure scenarios.

Exposure to dilute concentrations of bupropion affects zebrafish early life stages

Psychiatric pharmaceuticals are one of the most prescribed active substances globally. Bupropion (BPP) is an antidepressant that acts via inhibition of norepinephrine and dopamine reuptake. It has been found in various water matrices, and thus its effects on aquatic organisms must be studied. The present study aimed to evaluate the acute toxic effects of BPP on zebrafish (Danio rerio) early life stages. For developmental analysis, organisms were exposed for 168 h to concentrations ranging from 0 to 82000 μg/L. Two other experiments were performed by exposing embryos to a wide range of concentrations (from 0 to 50000 μg/L) in order to evaluate BPP effects on embryonic behavior, using the Zebrabox and testing at the biochemical level (acetylcholinesterase, glutathione-S-transferase, lactate dehydrogenase and catalase). Developmental analysis indicated that BPP had low acute toxicity with a calculated 168 h-LC50 of 50346 μg/L. Concentrations equal to or above 44800 μg/L elicited several effects such as hatching delay, edemas and tail deformities. However, concentrations from 7300 μg/L upwards elicited equilibrium alteration. Behavioral analysis showed that BPP affected zebrafish locomotor behavior by decreasing activity at 0.6 μg/L, increasing activity at 8.8 and 158 μg/L, and decreasing activity at 50000 μg/L. Biochemical analysis showed an increase of AChE activity at 158 and 2812 μg/L, an increase in GST at the highest concentrations, CAT alteration and increase of LDH at 0.6, 2812 and 50000 μg/L. We can conclude that BPP affects zebrafish early life stages at environmental concentrations.

Psychiatrics and selected metabolites in hospital and urban wastewaters: Occurrence, removal, mass loading, seasonal influence and risk assessment

The occurrence, removal, mass loading, seasonal influence and environmental risk assessment of nine psychiatric pharmaceuticals and four of their selected metabolites, were studied in one hospital and one urban wastewater treatment plant (WWTP) in Ioannina city, in northwestern Greece, providing information about the efficiency of the plants and their contribution into the final receiver's flow. Samples were collected from the influents and the effluents of the plants in different sampling campaigns, from July to December 2016. Analytical methodology was based on ultra-high performance liquid chromatography-Orbitrap high-resolution mass spectrometry, after solid-phase extraction through Oasis HLB cartridges. Concentrations in both WWTPs ranged between <LOQ and 1126.3 ng/L in the influents and between <LOQ and 1127.4 ng/L, in the effluents. Results indicated that venlafaxine and its metabolite O-desmethyl venlafaxine were the most frequently detected compounds in the influents and the effluents of both WWTPs. Metabolite to parent compound ratio ranged in the influents between 0.01 and 87.2 while in the effluents between 0.01 and 47.7. Based on mass loads in the influents, venlafaxine is consumed in high amounts (up to 67.1 mg/day/1000 inhab.), and consequently its metabolite O-desmethyl venlafaxine (up to 139.1 mg/day/1000 inhab.). Similarly to the influents, environmental emissions, were also higher for venlafaxine and O-desmethyl venlafaxine (10.1 and 13.3 mg/d/1000 inhab., respectively). Removal efficiencies in the hospital WWTP ranged from −100% to 98.9%, while in the city WWTP from −49.9% to 99.8%. Furthermore, an important outcome was the evaluation of the potential ecotoxicological risk, by means of risk quotients (RQs), where none of the target psychiatrics or their metabolites showed RQ above 1.

The fate and risk assessment of psychiatric pharmaceuticals from psychiatric hospital effluent

Psychiatric pharmaceuticals are gaining public attention because of increasing reports of their occurrence in environment and their potential impact on ecosystems and human health. This work studied the occurrence and fate of 15 selected psychiatric pharmaceuticals from 3 psychiatric hospitals effluent in Shanghai and investigated the effect of hospitals effluent on surface water, groundwater, soil and plant. Amitriptyline (83.57ngL−1) and lorazepam (22.26ngL−1) showed the highest concentration and were found frequently in hospital effluent. Lorazepam (8.27ngL−1), carbamazepine (83.80ngL−1) and diazepam (79.33ngL−1) showed higher values in surface water. The concentration of lorazepam (46.83ngL−1) in groundwater was higher than other reports. Only six target compounds were detected in all three soil points in accordance with very low concentration. Alkaline pharmaceuticals were more easily adsorbed by soil. Carbamazepine (1.29ngg−1) and lorazepam (2.95ngg−1) were frequently determined in plant tissues. The correlation analyses (Spearman correlations > 0.5) showed the main source of psychiatric pharmaceuticals pollutants might be hospital effluents (from effluent to surface water; from surface water to groundwater). However, hospital effluents were not the only pollution sources from the perspective of the dilution factor analysis. Although the risk assessment indicated that the risk was low to aquatic organism, the continuous discharge of pollution might cause potential environment problem.

Single and multi-component adsorption of psychiatric pharmaceuticals onto alternative and commercial carbons

This work describes the adsorptive removal of three widely consumed psychiatric pharmaceuticals (carbamazepine, paroxetine and oxazepam) from ultrapure water. Two different adsorbents were used: a commercial activated carbon and a non-activated waste-based carbon (PS800-150-HCl), produced by pyrolysis of primary paper mill sludge. These adsorbents were used in single, binary and ternary batch experiments in order to determine the adsorption kinetics and equilibrium isotherms of the considered pharmaceuticals. For the three drugs and both carbons, the equilibrium was quickly attained (with maximum equilibrium times of 15 and 120 min for the waste-based and the commercial carbons, respectively) even in binary and ternary systems. Single component equilibrium data were adequately described by the Langmuir model, with the commercial carbon registering higher maximum adsorption capacities (between 272 ± 10 and 493 ± 12 μmol g−1) than PS800-150-HCl (between 64 ± 2 and 74 ± 1 μmol g−1). Multi-component equilibrium data were also best fitted by the single component Langmuir isotherm, followed by the Langmuir competitive model. Overall, competitive effects did not largely affect the performance of both adsorbents. Binary and ternary systems maintained fast kinetics, the individual maximum adsorption capacities were not lower than half of the single component systems and both carbons presented improved total adsorption capacities for multi-component solutions.

Occurrence and fate of psychiatric pharmaceuticals in the urban water system of Shanghai, China

Psychiatric pharmaceuticals are the most prescribed active substances throughout the world and their presence in the environment raised concerns. The occurrence and fate of 15 selected psychiatric pharmaceuticals, including eight benzodiazepines, four antidepressants, one antiepileptic and two metabolites of benzodiazepines were investigated in wastewater treatment plant (WWTP) influents and effluents, surface water, and final drinking water in Shanghai. Psychiatric pharmaceuticals were in WWTPs influents ranging from low ngL−1 to 68.2ngL−1, dominated by carbamazepine, doxepin, diazepam and lorazepam. Target analytes were still detected in effluents from low ngL−1 range to 47.3ngL−1, with carbamazepine, diazepam, and oxazepam as most prevalent. WWTPs were low effective (<50%) in removing most of them, excluding amitriptyline (mean 60%), doxepin (mean 70%), temazepam (mean 78%) and lorazepam (mean 93%). In addition, carbamazepine, diazepam, oxazepam and lorazepam were detected in low ngL−1 to 75.5ngL−1 in the surface water of Huang Pu Rive. The pattern of contaminants in surface water is similar to the effluent wastewater, which suggested the main source of organic trace pollutants might be WWTPs. Furthermore, carbamazepine (0.8–2.5ngL−1), diazepam (0.5–3.2ngL−1) and alprazolam (2.3ngL−1) were also detected in drinking water and the concentrations were below the health based precautionary value. The investigation was within the range of those results reported in other countries. Our results indicate ubiquity of the investigated compounds in the aquatic system. These pollutants may potentially reach drinking water via WWTP effluents and/or surface waters and require constant attention.

Psychotropic drugs in mixture alter swimming behaviour of Japanese medaka (Oryzias latipes) larvae above environmental concentrations.

Psychiatric pharmaceuticals, such as anxiolytics, sedatives, hypnotics and antidepressors, are among the most prescribed active substances in the world. The occurrence of these compounds in the environment, as well as the adverse effects they can have on non-target organisms, justifies the growing concern about these emerging environmental pollutants. This study aims to analyse the effects of six psychotropic drugs, valproate, cyamemazine, citalopram, sertraline, fluoxetine and oxazepam, on the survival and locomotion of Japanese medaka Oryzias latipes larvae. Newly hatched Japanese medaka were exposed to individual compounds for 72 h, at concentrations ranging from 10 μg L(-1) to 10 mg L(-1). Lethal concentrations 50 % (LC50) were estimated at 840, 841 and 9,136 μg L(-1) for fluoxetine, sertraline and citalopram, respectively, while other compounds did not induce any significant increase in mortality. Analysis of the swimming behaviour of larvae, including total distance moved, mobility and location, provided an estimated lowest observed effect concentration (LOEC) of 10 μg L(-1) for citalopram and oxazepam, 12.2 μg L(-1) for cyamemazine, 100 μg L(-1) for fluoxetine, 1,000 μg L(-1) for sertraline and >10,000 μg L(-1) for valproate. Realistic environmental mixture of the six psychotropic compounds induced disruption of larval locomotor behaviour at concentrations about 10- to 100-fold greater than environmental concentrations.

Simultaneous Determination of 13 Psychiatric Pharmaceuticals in Sewage by Automated Solid Phase Extraction and Liquid Chromatography-Mass Spectrometry

Abstract A method has been developed for the determination of 13 psychiatric pharmaceuticals in wastewater by automated solid phase extraction-high performance liquid chromatography coupled with electrospray ionization tandem mass spectrometry (ASPE-HPLC-ESI-MS/MS) in positive ion mode and multiple reactions monitoring (MRM). The ASPE extracts were further purified using an amino SPE cartridge prior to HPLC analysis. HPLC separation was performed on an Waters X-bridge C18 column utilizing a gradient elution program of the organic mixture (acetonitrile-methanol, 50:50, V/V) and ammonium formate buffer solution (containing 0.2% formic acid, and adjusted to pH 3.5 with ammonium hydroxide) as the mobile phase. The recoveries of all analytes from matrix spiking experiments in the sewage WWTP's influent and effluent at three concentrations ranged from 93.2% to 106.8%. The matrix effects were 77.3%–98.6% except that for citalopram. Method detection limits for the analytes in the sewage WWTP's influent and effluent were