Prunella Modularis Research Articles

First detection and a new avian host of the tick Ixodes ventalloi Gil Collado, 1936, in Slovakia

This study describes the first detection of Ixodes ventalloi in Slovakia. Two engorged females of I. ventalloi were collected from Dunnocks (Prunella modularis) captured in eastern Slovakia. The identification of females was based on morphological and molecular 16S rRNA gene features. Phylogenetic analysis revealed a classification of the females into distinct genogroups. Moreover, comparative morphological analysis highlighted variations between the two females, particularly in the curvature of the auriculae, the shape of coxa I, and the internal spur. These findings suggest the potential for varied phenotypes of I. ventalloi correlated with their genogroups. Nonetheless, I. ventalloi population establishment within Slovakia necessitates further investigation through flagging or drag sampling.

Constitutive immune function is not associated with fuel stores in spring migrating passerine birds.

Migratory birds may either upregulate their immune system during migration as they might encounter novel pathogens or downregulate their immune system as a consequence of trade-offs with the resource costs of migration. Support for the latter comes not least from a study that reports a positive correlation in autumn migrating birds between fuel stores and parameters of innate and acquired immune function, that is, energy-exhausted migrants appear to have lowered immune function. However, to our knowledge, no study has tested whether this pattern exists in spring migrating birds, which may face other trade-offs than autumn migrants. Here, we investigate if in spring there is a relationship between fuel stores and microbial-killing ability, a measure of innate immune function, and total immunoglobulin (IgY), a measure of acquired immune function, in four migrating bird species: chaffinches (Fringilla coelebs), dunnocks (Prunella modularis), song thrushes (Turdus philomelos) and northern wheatears (Oenanthe oenanthe). Our findings indicate no significant correlation between fuel stores and either microbial killing ability or IgY levels when considering all species collectively. When analysing species separately, we found a significant negative correlation between fuel stores and microbial-killing ability in chaffinches and a positive correlation between fuel stores and IgY levels in wheatears. In song thrushes, but not in any of the other species, there was a significant negative correlation between relative arrival date and microbial-killing ability and between arrival date and IgY levels. Sex did not affect immune function in any of the species. Our study suggests that the relationship between immune function and fuel stores may be different during spring migration compared to autumn migration. Differences in the speed of migration or pathogen pressure may result in different outcomes of the resource trade-off between investment in immune function and migration among the seasons.

Effects of habitat heterogeneity on bird communities in the forests of northeastern Germany

Habitat heterogeneity is a key factor for regulating biodiversity in temperate lowland forests. Specifically, stands associated with late forest development phases provide important habitat structures for many rare and threatened forest bird species. However, how forest stands that differ in their structural complexity, canopy conditions and tree species composition affect forest bird assemblages both at the local and landscape scale remains largely unclear. In a young moraine lake landscape of northeastern Germany, we assessed correlations of bird diversity and bird composition with stand properties. We used data from 48 transects (400 m) established in (1) unmanaged, closed-canopy, mature stands that were dominated by Fagus sylvatica (UDS), and (2) diverse managed, mixed coniferous stands with a mosaic of open and closed canopy patches (MCS). We found that bird communities of the UDS strongly differed from those in the MCS, with open habitat species being more frequent in the MCS. By contrast, differences in diversity measures were less distinct. Moreover, we identified nine indicator species for the UDS (Columba oenas, Cyanistes caeruleus, Muscicapa striata, Leiopicus medius, Certhia brachydactyla, Ficedula parva, Dryobates minor, Sturnus vulgaris, Ficedula hypoleuca) and seven indicator species for the MCS (Periparus ater, Pyrrhula pyrrhula, Regulus regulus, Prunella modularis, Lophophanus cristatus, Emberiza citronella, Anthus trivialis). Several famous ancient beech forest patches in Müritz National Park and the UNESCO Biosphere Reserve “Schorfheide-Chorin” were found to harbour the set of the UDS indicator species. The UDS support of bird coenosis is typical for old mature broadleaved forests and can be considered as an effective tool for bird conservation. Our results further indicate that the combination of unmanaged and managed stands associated with different habitat complexities would benefit forest bird assemblages at the landscape scale. Key words: forest specialist birds, bird conservation, non-managed forests, old-growth patches, Ficedula parva, Leiopicus medius, coniferous forest

A preliminary study of the winter roosting behaviour of four woodland passerines

ABSTRACT Capsule Radio-tracking of four woodland passerine species reveals variation in winter roost site selection within and between species. Aims To evaluate methods for studying winter roosting behaviour in woodland passerines and to collect preliminary data on intra- and inter-specific differences in roost site characteristics and spatial arrangement. Methods Ten woodland birds (three Eurasian Blackbirds Turdus merula, three Dunnocks Prunella modularis, two Great Tits Parus major and two European Robins Erithacus rubecula), were fitted with a tail-mounted Lotek PicoPip AG337 VHF radio tag (January to March 2022). Tagged birds were located by triangulation, and nightly winter roost locations identified and characterized. A geographic information system was used to quantify roost site fidelity, roosting height and types of habitats used. Results Manual tracking of birds with radio tags is a cost effective and appropriate method for studying passerine winter roosting behaviour. Sample sizes were too small to allow exploration of significant differences between sites, age and sex. European Robins showed the greatest site fidelity in relation to between-night roost positions, with Eurasian Blackbird, Great Tit and Dunnock being more variable in the sites chosen between nights. Eurasian Blackbird and Great Tit roost sites were generally higher above the ground (up to 6 m), compared to European Robins and Dunnocks (all sites less than 4 m above the ground). Eurasian Blackbirds varied the most in the number of habitats used for roost sites, and European Robins showed the least variation. Only three out of 11 habitat types (bramble Rubus sp, laurel Prunus sp. and Sycamore Acer pseudoplatanus with Ivy Hedera helix) were used by more than one species as a roosting habitat. Conclusion Further research should focus on: (1) habitat use in relation to relative availability; (2) increasing samples sizes to allow comparison of factors such as age, sex and sites; (3) the impact of supplementary feeding on roosting behaviour; (4) quantifying the thermal properties of roost sites.

Bird communities after 37 years in a rural area of NW Spain

In a globalized world, changes in human activities are negatively affecting biodiversity at different scales. Long-term monitoring of bird communities can inform about changes in biodiversity over time. We explored how bird species richness, and abundance changed after a 37-year period at 5 sites in O Courel Mountains, a well conserved region of NW Spain. We also analyzed the abundance changes over time in the 15 most abundant species and whether these changes relate to the phenotypes of these species. Repeated bird surveys were performed in the same locations of five different sites in 1977 and 2014. Our results showed species richness and overall bird abundance did not change after 37 years within the five studied sites. Although, within species analyses showed 8 species changed their abundances. Overall, Cyanistes caeruleus, Garrulus glandarius, Parus major, Prunella modularis and Sylvia atricapilla increased their abundances and Erithacus rubecula, Phylloscopus ibericus, and Troglodytes troglodytes decreased over time. We did not find any relationship between the change in species abundances and bird phenotypes. This study shows that, despite the specific changes in the abundance of eight species over nearly four decades, overall bird species richness and abundance may be maintained in well preserved natural areas.

In the bleak mid-winter: The value of brownfield sites for birds during the winter

Brownfield sites (e.g., wastelands, disused industrial grounds and power stations) are valuable refuges for urban birds during the breeding season. This owes to the presence of vegetation in different stages of succession, which provide a high diversity of food and breeding resources. However, insufficient research has explored their winter value, when temperatures are lower and food is scarcer. Accordingly, 342 bird and habitat surveys were conducted in brownfield sites, and other land-uses, across Greater Manchester. Bird density estimates were then calculated using Distance software and bird-habitat associations were analysed using Canonical Correspondence Analysis (CCA). Moreover, the impact of brownfield site development on winter bird density from 2000 to 2009 in Greater Manchester was explored using the bird density estimates, and historical image function in Google Earth. In brownfield sites, Pica pica had the highest density estimate (142 per km2), although four species had estimated densities > 100 per km2 (Turdus merula; Cyanistes caeruleus; Passer domesticus; Aegithalos caudatus). The CCA indicated that Aegithalos caudatus, Cyanistes caeruleus, Parus major, Prunella modularis were associated with Fagus sylvatica, Betula pendula, and Buddleja davidii on brownfield sites, which during the winter 1) produce seeds 2) support overwintering insects, providing a valuable food source. Nevertheless, across land-uses, most species favoured green space and pre-2000 housing. Brownfield sites were most replaced between 2000 and 2009, with 17.8 km2, 14.9 km2, and 12 km2 replaced by commercial, industrial, and post-2000 housing respectively. This is likely to have had a negative impact on the winter density of most bird species, owing to 1) the removal of brownfield site vegetation 2) increases in impervious land cover. Future declines in winter bird abundance could be mitigated by retaining brownfield site vegetation and setting aside brownfield sites in close proximity to green space and pre-2000 housing.

Godman‐Salvin Prize

Godman‐Salvin Prize

Composition of nests constructed by species in the Motacillidae, Sylviidae and Prunellidae

Bird nests can be complex bio-engineered structures constructed from a range of materials to provide a site for incubation, and in many species chick rearing. Reports of the materials used in nest walls and cup linings are typically qualitative and do not assist in understanding the functional properties of the structure as a whole. This paper provides size and composition data for nests from four species of the Motacillidae family: the meadow pipit ( Anthus pratensis), pied wagtail ( Motacilla alba), grey wagtail ( Motacilla cinerea), and the yellow wagtail ( Motacilla flava), three species of the Sylviidae family: willow warbler ( Phylloscopus trochilus), Eurasian reed warbler ( Acrocephalus scirpaceus) and Eurasian blackcap ( Sylvia atricapilla), and the dunnock ( Prunella modularis) of the Prunellidae family. Three hypotheses were tested: do nest dimensions correlate with body mass? Is it possible to distinguish among species based on their nest composition; and for individual species? Is it possible to distinguish between the cup lining and outer nest wall based on the materials used in construction? Nest composition and size varied among species although the degree of intra-specific variation was different. Size of a nest was unrelated to average female adult body mass. Composition relied on similar types of materials but in differing quantities between species. It was possible to distinguish among species in terms of nest construction, and between the cup lining and the outer nest, on the basis of at least one of the component materials. By providing quantitative data on composition, it may be possible in the future to understand better the structural, insulative and hydrological properties of the nest. Such information will be invaluable as we begin to develop an understanding of the factors that have driven evolution of nest architecture.

The association between personalities, alternative breeding strategies and reproductive success in dunnocks.

Although consistent between-individual differences in behaviour (i.e. animal personality) are ubiquitous in natural populations, relatively few studies have examined how personalities influence the formation of social relationships. Yet, behavioural characteristics of both sexes might be key when it comes to pair-bond formation, and cooperation with partners to successfully rear offspring. We here use a wild population of dunnocks (Prunella modularis) to first investigate whether individuals mate nonrandomly (i.e. assortative mating) with regard to four behavioural traits-flight-initiation distance (FID), provisioning, activity and vigilance-that differ in repeatability and have previously been associated with mating patterns and fitness in other species. Second, we test whether an individual's FID is associated with variability in the dunnocks' mating system (i.e. monogamous pairs vs. polygamous groups). Finally, we determine whether FID and provisioning of males and females associate with their reproductive success. We found no statistical support for assortative mating in FID between males and females. Interestingly, in polygamous groups, co-breeding males differed in their FIDs with dominant alpha males having significantly shorter FIDs compared with subordinate beta-males. Moreover, there was evidence for assortative mating in provisioning for alpha males and females in polygamous groups. We also found that male provisioning influenced reproductive success of both sexes, whereas female provisioning rates only positively correlated with her own but not their partner(s) reproductive output. Our results suggest that personality differences may have important implications for social relationships, the emergence of different mating patterns and ultimately reproductive success within populations.

Elevation of two subspecies of Dunnock Prunella modularis to species rank

The Western Palearctic endemic Dunnock Prunella modularis was recently revealed to comprise three distinct genetic lineages, each distributed in different Pleistocene refugia. Specifically, one is isolated in the Iberian refugium, another is confined to the Caucasus refugium, and the third is distributed in both the Italian and Balkan refugia, as well as across broader Europe. There is a probable absence of gene flow between the refugia. Analysis of plumage and song characteristics reveals robust differences between the Iberian subspecies P. m. mabbotti, Caucasian P. m. obscura and nominate P. m. modularis. Our assessments, in conjunction with genetic isolation, support species recognition under the Phylogenetic, Biological and Comprehensive Biological Species Concepts, via qualitative and quantitative criteria, and diagnosability. We thus propose the elevation of Iberian Dunnock P. mabbotti and Caucasian Dunnock P. obscura to species level.

Atypical Knemidokoptosis in Two Dunnocks (Prunella modularis) in Southern England.

Avian knemidokoptosis, caused by knemidokoptid mites (Knemidokoptinae: Epidermoptidae), has been reported in wild and domestic birds globally. We report two cases of severe knemidokoptosis in Dunnocks (Prunella modularis) from separate sites in Great Britain, where the disease has previously been reported predominantly in finches and, less frequently, in corvids.

Tongue spots of Dunnock (Prunella modularis) nestlings reflect body condition but exert only conditional influence on parental allocation

The nestlings of many bird species have ornaments in their mouths, yet the within‐species function of these ornaments remains relatively little studied compared with adult ornaments. Here, we describe the pattern of variation in the tongue spots of Dunnock Prunella modularis nestlings and evaluate their potential influence on parental allocation. We observed that some nestlings hatch with a third tongue spot at the tip of the tongue, which then fades as nestlings age. The number of tongue spots present is inversely related to nestling weight, but the number of tongue spots present does not appear consistently to influence parental allocation. This suggests the need for further investigations into within‐species variation of mouth marks in nestling birds to pave the way to a better understanding of these intriguing ornaments.

Species turnover in forest bird communities on Fiordland islands following predator eradications

Recent advances in the control of mammalian predators have begun to reveal interspecific competition as a key driver in the structure of New Zealand forest bird communities once predation pressure is reduced. We present evidence that, when at high densities, South Island robins (Petroica australis) may be responsible for declines in a suite of smaller native and introduced songbird species. Bird surveys undertaken on 47 islands in Breaksea Sound and Dusky Sound, Fiordland, during 1974 to 1986, were repeated on the same islands in 2016 or 2019. During the first block of surveys, Norway rats (Rattus norvegicus) were present on two islands, and stoats (Mustela erminea) were known or presumed to regularly reach 43 of the remaining islands. The rats were eradicated in 1986 and 1988, and stoats have been controlled to zero density since 2001 on all 28 of the islands surveyed in Dusky Sound, and since 2008 on 12 of 19 islands surveyed in Breaksea Sound. Bird species that apparently benefited from pest mammal eradications included South Island robin (Petroica australis) and kākā (Nestor meridionalis), both of which are endemic. Species recorded less often after the eradications included tomtit (Petroica macrocephala), grey warbler (Gerygone igata), silvereye (Zosterops lateralis), dunnock (Prunella modularis), and chaffinch (Fringilla coelebs) – a mixture of endemic, native, and introduced species. We hypothesise that these five species have been outcompeted or displaced by the now widespread and abundant South Island robin, probably through aggressive interactions.

Exposure of passerine birds to brodifacoum during management of Norway rats on farms

The exposure of non-target wildlife to anticoagulant compounds used for rodent control is a well-known phenomenon. Exposure can be primary when non-target species consume bait or secondary via uptake of poisoned animals by mammalian and avian predators. However, nothing is known about the exposure patterns in passerine birds that are commonly present on farms where rodent control is conducted.We used liquid chromatography coupled with tandem mass spectrometry to screen for residues of anticoagulant rodenticides (ARs) in liver tissue of passerine birds that were present during rodent control with a product containing brodifacoum (BR). The 222 birds of 13 species were bycatch of rodent snap trapping in 2011–2013 on 11 livestock farms run synchronously with baiting.During baiting, ARs were detected in about 30% of birds; 28% carried BR. In liver tissue of 54 birds that carried BR, concentrations ranged from 4 to 7809 ng/g (mean 490 ± 169 ng/g). Among common bird species with AR residues, BR was most prevalent in robins (Erithacus rubecula) (44%) and dunnocks (Prunella modularis) (41%). Mean BR concentration was highest in great tits (Parus major) (902 ± 405 ng/g). The occurrence and concentrations of BR residues were about 30% higher in birds collected close to bait stations compared to birds collected further away.The results demonstrate that several ground feeding songbird species are exposed to ARs used on farms. If BR was present in liver tissue, concentrations were variable, which may imply a combination of primary and secondary exposure of songbirds. Exposure was mostly restricted to the immediate surroundings of farms where bait was used, which might limit the transfer to the wider environment. Efforts should be made to reduce the access for birds to AR bait to prevent high exposure.

Assessment of the dunnocks’ introduction to New Zealand using innate immune-gene diversity

Immunity genes are proposed to be informative about evolutionary processes acting upon introduced populations (e.g., showing signatures of selection). This is because immunity genes are expected to be under pathogen-mediated selection, and this type of selection can be more pronounced when individuals are exposed to new environmental conditions. Here we assessed innate immune genetic diversity, via Toll-like receptors (TLRs), to quantify genetic differentiation between a deliberately introduced population of dunnocks (Prunella modularis) in New Zealand and its source population in the United Kingdom. We also asked whether the introduced population shows signatures of intergenerational and current selection in TLR. We expect intergenerational and current selection patterns because New Zealand dunnocks have been exposed to new environmental conditions following their introduction around the late 1800s, which may have driven pathogen-mediated selection. Counter to our expectation, we found only weak and non-significant genetic differentiation between the introduced and source populations. Further, the levels of genetic differentiation (G ST ′ ) found in TLRs were similar to those found in microsatellites across the populations. Dunnocks, in general, have been under strong purifying selection over evolutionary time, but we found little evidence to support signatures of contemporary selection in TLR in the introduced population. Notably, however, we found a statistically significant heterozygosity advantage for males in TLR3, which lends support to possible current selection acting upon the introduced population. Overall, it appears that New Zealand dunnocks have retained a high proportion of the immunogenetic diversity of the source population, and that such diversity has probably been sufficient to defend against the potential pathogens found in New Zealand. Our results may explain—at least in part—why the introduction of dunnocks has been so successful; dunnocks have become one of the most common birds in New Zealand.

Dunnock social status correlates with sperm speed, but fast sperm does not always equal high fitness.

Sperm competition theory predicts that males should modulate sperm investment according to their social status. Sperm speed, one proxy of sperm quality, also influences the outcome of sperm competition because fast sperm cells may fertilize eggs before slow sperm cells. We evaluated whether the social status of males predicted their sperm speed in a wild population of dunnocks (Prunella modularis). In addition to the traditional analysis of the average speed of sperm cells per sample, we also analysed subsamples of the fastest sperm cells per sample. In other words, we systematically evaluated the effects of including different numbers of the fastest sperm in our analyses, ranging from the 5-fastest sperm cells to the 100-fastest sperm cells in a sample. We further evaluated whether fitness, defined here as the number of chicks sired per male per breeding season, relates to the sperm speed in the same population. We found that males in monogamous pairings (i.e. low levels of sperm competition), produced the slowest sperm cells, whereas subordinate males in polyandrous male-male coalitions (i.e. high levels of sperm competition) produced the fastest sperm cells. This result was consistent regardless of the number of fastest sperm included in our analyses, but statistical support was conditional on the number of sperm cells included in the analysis. Interestingly, we found no significant relationship between fitness and sperm speed, which suggests that it is possible that the differential mating opportunities across social status levelled out any possible difference. Our study also suggests that it is important to identify biologically meaningful subsets of fastest sperm and cut-offs for inclusions for assessing sperm competition via sperm speed.

Responses of New Zealand forest birds to management of introduced mammals.

Over the past 1000 years New Zealand has lost 40–50% of its bird species, and over half of these extinctions are attributable to predation by introduced mammals. Populations of many extant forest bird species continue to be depredated by mammals, especially rats, possums, and mustelids. The management history of New Zealand's forests over the past 50 years presents a unique opportunity because a varied program of mammalian predator control has created a replicated management experiment. We conducted a meta‐analysis of population‐level responses of forest birds to different levels of mammal control recorded across New Zealand. We collected data from 32 uniquely treated sites and 20 extant bird species representing a total of 247 population responses to 3 intensities of invasive mammal control (zero, low, and high). The treatments varied from eradication of invasive mammals via ground‐based techniques to periodic suppression of mammals via aerially sown toxin. We modeled population‐level responses of birds according to key life history attributes to determine the biological processes that influence species’ responses to management. Large endemic species, such as the Kaka (Nestor meridionalis) and New Zealand Pigeon (Hemiphaga novaeseelandiae), responded positively at the population level to mammal control in 61 of 77 cases for species ≥20 g compared with 31 positive responses from 78 cases for species <20 g. The Fantail (Rhipidura fuliginosa) and Grey Warbler (Gerygone igata), both shallow endemic species, and 4 nonendemic species (Blackbird [Turdus merula], Chaffinch [Fringilla coelebs], Dunnock [Prunella modularis], and Silvereye [Zosterops lateralis]) that arrived in New Zealand in the last 200 years tended to have slight negative or neutral responses to mammal control (59 of 77 cases). Our results suggest that large, deeply endemic forest birds, especially cavity nesters, are most at risk of further decline in the absence of mammal control and, conversely suggest that 6 species apparently tolerate the presence of invasive mammals and may be sensitive to competition from larger endemic birds.

A Case Study on Wild Birds: A Human Enteric Pathogens Transmission

Wild birds have been shown to be vectors for enteric pathogens, they may constitute an environmental carrier of pathogens representing a source of human infection. The study aims to evaluate the intestinal carriage of Salmonella spp, Campylobacter spp and Yersinia enterocolitica in wild birds in the Italian Regional Natural Park of “Lagolungo and Ripasottile” lakes. A total of 276 samples from 16 bird species were sampled and tested by classical cultural methods and the presence of Salmonella and Y. enterocolitica was detected, using real time PCR as screening methods. None of the fecal samples were positive for Y. enterocolitica. Campylobacter spp was isolated from 4 fecal specimens and Salmonella positive results were obtained from three avian species. Four different colonies of S. Napoli isolated from Cettia cetti were submitted to molecular typing by PFGE, all showing the same genetic pattern and sharing 70-80% homology with the 3 representative human isolates. One strain of S. Typhimurium from Nycticorax nyctcorax and one strain of S. Livingstone from Sylvia atricapilla were also isolated. Campylobacter spp. were isolated from Phylloscopus collybita, Cyanistes caeruleus, Prunella modularis, Carduelis chloris chloris. Wild birds, might cause the contamination of vegetables crops either directly with faecal material, or indirectly, with pollution of irrigation water. The study demonstrated that the molecular platforms to detect Salmonella and Y. enterocolitica seem to be very appealing as screening methods. Enteric pathogen circulation associated to wild fauna strictly linked to water ecosystems open new scenarios of management agricultural practices.

The effect of urbanization on population densities of forest passerine species in a Central European city

Abstract Typical, but less common, passerine forest species were selected for this study, such as Lullula arborea, Anthus trivialis, Troglodytes troglodytes, Prunella modularis, Turdus philomelos, Turdus viscivorus, Phylloscopus sibilatrix, Regulus regulus, Regulus ignicapillus, Muscicapa striata, Ficedula albicollis, Ficedula hypoleuca, Parus cristatus, Parus palustris, Parus ater, Certhia familiaris, Certhia brachydactyla, Oriolus oriolus, Garrulus glandarius, and Corvus corax. M. striata and T. philomelos were the most numerous among the 20 investigated species, the former one nested in a density of 6.7 pairs per 100 ha of wooded area, while the later one at 5.1 pairs per 100 ha. Density of most other species was below 3 pairs per 100 ha of wooded area. A. trivialis, P. cristatus and P. modularis were unexpectedly rare (&lt; 1 pair per 100 ha). Otherwise, relatively numerous were T. troglodytes (1.8 p./100 ha), R. regulus (1.8 p./100 ha) and P. palustris (1.4 p./100 ha). P. cristatus, L. arborea, and T. viscivorus were the rarest species investigated (below 0.1 p./100 ha). Several bird species nested in wooded areas only in the outer zone of the city. This group included A. trivialis, R. regulus, P. ater, and C. corax. Population density of T. troglodytes, T. philomelos and O. oriolus were significantly higher in outer than in inner zone, while the reverse was true in the case of M. striata and F. hypoleuca.

Domestic Cat Predation on Garden Birds: An Analysis from European Ringing Programmes

Domestic Cat Felis silvestris catus populations have been increasing since the 1970s in Western Europe. This increase is particularly notable in human habitats, such as gardens and parks, where Cats have locally become the most abundant carnivore. In urban contexts, understanding Cat behaviour could be important for biodiversity management. In this study, we analysed Cat predation on garden birds, using existing data from ringing programmes in France and Belgium. These types of data permit us to (1) investigate the bird species killed by Cats, (2) analyse changes in the proportion of birds killed by Cats over the years 2000–2015, and (3) compare the causes of bird mortality as reported by observers. The most affected species were passerines, both granivorous and insectivorous (Europian Robin Erithacus rubecula, Dunnock Prunella modularis, Greenfinch Carduelis chloris), who can frequently be found feeding on the ground or visiting feeders in the winter. Perched birds (Chiffchaff Phylloscopus collybita, Blackcap Sylvia atricapilla) were less represented. Ground-dwelling habits were significantly correlated to predation rates. Between 2000 and 2015, Cat-related mortality in garden birds increased by at least 50%, depending on the ringing programme studied, which coincided with an increase in Cat populations. Cat-related mortality is one of the leading causes of death reported by observers (12.8–26.3% of total dead birds) and occurs at the same order of magnitude as deaths due to collisions with windows. These results underline the need to consider Cats and their potential impacts on urban biodiversity management at garden and urban levels.