Proximate Composition Research Articles

Development of gluten free crackers using water chestnut (Trapa natans) and Pearl Millet (Pennisetum glaucum)

Baked goods are favored by the public due to their widespread availability, convenience, ready-to-eat nature, and excellent storage capabilities. The study focused on developing high-quality gluten-free crackers by incorporating pearl millet (Bajra) and water chestnut flour which enhances nutritional value and is gluten free, catering to individuals with celiac disease.To Formulate Crackers with Pearl Millet and water chestnut flour. To determine the Sensory quality of the developed product. To Estimate the Proximate composition and cost of the developed product.Pearl millet flour, water chestnut flour, whole wheat flour,salt, jaggery, garlic powder, ajwain, mixed herbs, oil, and baking powder were procured from local grocery stores of Mysore and standard A.O.A.C (2005) methods for Proximate analysis and 9-point hedonic scale was used for sensory analysis.: The sensory evaluation revealed that PMC4 was highly acceptable on par with control. Proximate analysis showed that PMC4 had high phosphorous and calcium content.The study successfully developed superior gluten-free crackers with incorporation of Pearl millet and water chestnut flour. Among all the variations PMC4 was rich in phosphorous and calcium and is gluten free which caters to individuals with celiac disease.

Proximate Composition of Some Fish Feed Samples for Small-scale Aquaculture Farmers in Bobasi Sub-County, Kisii County, Kenya

Proximate Composition of Some Fish Feed Samples for Small-scale Aquaculture Farmers in Bobasi Sub-County, Kisii County, Kenya

Physicochemical Properties of Fortified Coconut Milk Based Chocolate - Like Drinks as Influenced by Cocoa Powder and Sugar Levels

The physicochemical, proximate, vitamins and minerals composition of fortified drinks produced from coconut milk, cocoa powder, and sugar was evaluated. Drinks samples were formulated with different concentration of coconut milk, cocoa powder and sugar levels. Samples includes: “k1 (Coconut milk 100 %), k2 (Coconut milk 99.8 % cocoa powder 0.2 %), k3 (Coconut milk 99.6 %, cocoa powder 0.4%), k4 (Coconut milk 98 %, sugar 2%), k5 (Coconut milk 97.8 %, cocoa powder 0.2%, sugar 2%), k6 ( Coconut milk 97.6 %, cocoa powder 0.4%, sugar 2%), k7 (Coconut milk 96 %, sugar 4%), k8 (Coconut milk 95.8 %, cocoa powder 0.2%, sugar 4%), k9 (Coconut milk 95.6 %, cocoa powder 0.4%, sugar 4%)”. The samples were produced and subjected to physicochemical, proximate, vitamins and minerals analysis using standard methods. Physicochemical composition, pH ranged from 6.50 (k1) to 6.72 (k9), TTA from 0.23 (k9) to 0.41 % (k1), specific gravity from 1.03 % (k1) to 1.13 % (k9), total solid from 18.91(k1) to 22.52 g/100g (k9), SNF from 7.23 (k2) to 10.25 g/100g (k8) and energy values from 124.63 (k1) to 148 Kcal/100g (k9). The proximate composition; moisture from 77.48 % (k9) to 81.09 % (k1), protein from 3.42 (k1) to 3.96 % (k9), ash from 0.71(k1) to 0.98 %(k9), crude fiber from 0.02 % (k1) to 0.06 % (k9), fat from 10.23 % (k1) to 12.27% (k9) and carbohydrate from 4.82 to 5.58% for k1 and k9. Vitamin composition, vitamin A from 1.725 to 2.469mg/100g for samples k1 and k2, Vitamin B1 from 0.044 mg/100g (k1) to 0.205 mg/100g (k9), B2 from 0.028 to 0.0114 mg/100g for k1 and k9, vitamin C from 2.981 to 5.751mg/100g for k1 and k9 and vitamin K from 0.141 to 1.137 mg/100g for k1 and k9. Mineral composition of drinks: Calcium ranged from 16. 31 to 27.05mg/100g for k1and k9, Sodium from 15.25 to 16.99 mg/100g for k1 and k9, Magnesium from 37.20 to 72.64 mg/100g for k1 and k9, Zinc from 0.677 to 3.18 mg/100g for k1 and k9, Iron from 2.87 to 3.38 mg/100g for k1 and k2, Iodine from 0.16 to 0.28 mg/100g for k1 and k9.The Cocoa powder and sugar levels improved the physicochemical, proximate, vitamins, minerals and overall nutritional profile of fortified coconut milk based chocolate drinks.

Effects of porcine somatotropin administration on the responses to dietary lysine and a near-ideal blend of amino acids on the amino acid composition of whole-body protein and amino acid accretion rate in growing pigs.

An experiment was conducted to assess the effects of porcine somatotropin (pST) on the responses to a near-ideal blend of AA on the AA composition of empty, whole empty body (WEB) protein and WEB essential AA accretion rate in pigs from 22 to 60kg BW. Forty Hampshire × Yorkshire gilts were individually penned and assigned to a 4 × 2 factorial arrangement of treatments consisting of 4 diets with and without pST injection. A fortified corn-soybean meal basal diet was formulated to contain 1.50% total Lys with Thr, Met, and Trp added to obtain a near-ideal blend of these AA relative to Lys. In 3 additional diets, Lys was reduced to 1.25, 1.00, and 0.75% by diluting the basal diet with cornstarch, cellulose, and sand such that the diets also contained the same ratios of AA. Pigs that received pST were administered a daily i.m. injection of 2mg of pST. At 60kg BW, the WEB (carcass, head, viscera, blood, nails, and hair) was ground and analyzed for proximate and AA composition. Administration of pST increased (P < 0.001) accretion rates of WEB protein and essential AA. Increasing dietary essential AA increased (quadratic, P < 0.03) accretion rate of WEB protein, His, Leu, Trp, and Val in pST-treated pigs, but not in untreated pigs. Lysine composition in the accreted WEB protein was not affected (P > 0.05) by dietary Lys. The efficiency of Lys utilization for WEB Lys accretion was linearly affected (P < 0.01) by dietary Lys. These results indicated that the dietary Lys needed to achieve maximum WEB Lys accretion is markedly increased by pST administration.

Potentially functional lactose-free ice cream with Lacticaseibacillus casei CSL3, ginger, and honey.

To develop and characterize a functional lactose-free ice cream with added ginger and honey, evaluate the survival of Lacticaseibacillus casei CSL3 under frozen storage and the simulated gastrointestinal tract (GIT), as well as antioxidant activity and product acceptability. The survival of Lacticaseibacillus casei CSL3 was evaluated for 180 days, under frozen storage, and GIT at 60 days. At 15 days of storage, proximal composition, antioxidant activity, color, pH, acidity, fusion, density, overrun, and sensory analysis were performed. Ice cream was an effective food matrix for maintaining the viability of CSL3, with concentrations > 7 log CFU g- 1 during storage and GIT. In addition, the analysis showed overrun and prebiotic characteristics through high values of antioxidant activity and phenolic compounds, good acceptability, and purchase intention. The product has satisfactory market potential (acceptance rate of 95.19% and purchase intention rate > 96%), and it could become another means of inserting probiotics in food.

Physicochemical characterisation and impact of Gryllus bimaculatus addition on gluten‐free flour blends

SummaryGryllus bimaculatus (field cricket) powder is a prospect in enriching gluten‐free food products' protein and mineral contents. However, the technological impacts of replacing common gluten‐free cereal flours with G. bimaculatus powder are still incipient. This study aimed to characterise nutritional composition, particle morphology, and particle size distribution of G. bimaculatus powder and the gluten‐free flours (maize, rice, finger millet and proso millet). Additionally, it investigated the effects of substituting these gluten‐free flours with 5%, 10%, 15% and 20% G. bimaculatus powder on proximate composition, hydration and colour properties. G. bimaculatus powder showed significantly (P ≤ 0.05) high protein (44.8 g/100 g) content and essential minerals such as zinc (208.5 mg/kg) and iron (132.2 mg/kg). Micrographs of G. bimaculatus powder revealed compact and heterogenous particles while the flours' particles varied in sizes, and the starch granules had different shapes, sizes and arrangements. Flour particles exhibited bi‐and trimodal size distributions ranging from 1.5 μm to 1100 μm with minor peaks preceding major peaks. Finger millet flour had a greater span of 4.12, while proso millet flour had a lower span of 1.97. Particle size correlated negatively with water absorption capacity (WAC) (r = −0.938) and oil absorption capacity (OAC) (r = −0.955), respectively. G. bimaculatus powder addition significantly increased (P ≤ 0.05) fat, protein and fibre contents, decreased (P ≤ 0.05) nitrogen‐free extracts, but had no effect on ash content. Hydration properties differed significantly (P ≤ 0.05) except for WAC in proso millet blends. Samples exhibited significantly higher (P ≤ 0.05) WAC than OAC in exception of G. bimaculatus powder and showed no emulsifying activities. Addition of G. bimaculatus powder significantly (P ≤ 0.05) increased colour differences, reduced redness (a*), yellowness (b*) and lightness. The use of G. bimaculatus powder demonstrates the potential to enhance the nutritional properties and modify the colour and hydration of gluten‐free flours in the development of novel gluten‐free foods.

The Influence of Organic Manures on Proximate Composition, Minerals, Anti-nutrients and Vitamins of Selected Legume Forage

This study investigated the influence of three organic manure on the nutritional composition of three leguminous forages - Soybeans, Cowpeas, and Groundnuts, cultivated in the coastal Rainforest of Akwa Ibom State, Nigeria. The four different organic manure used were poultry droppings, goat droppings, pig dung and a control (no manure application). Each organic manure represented a treatment group. Each group was replicated three times making a total of 12 experimental units. The data collected were subjected to analysis of variance using the Statistical Package for Social Science (SPSS). Proximate analysis revealed significant (p&lt;0.05) differences in moisture, fat, ash, protein, fiber, carbohydrates, and energy content. Manure type significantly (p&lt;0.05) affected protein, carbohydrate, and moisture content. Poultry Manure had superior performance in Cowpea Forage, while Control excelled in Soybean Forage. The anti-nutrient analysis revealed significant (p&lt;0.05) differences in tannin, oxalate, saponin, and phytate content, impacting nutritional quality. Tannin content varied significantly (Groundnut: 65.78 mg/100g, Soybean: 109.78 mg/100g, Cowpea: 37.62 mg/100g), as did oxalate (Groundnut: 393.18 mg/100g, Soybean: 185.20 mg/100g, Cowpea: 357.13 mg/100g), saponin (Groundnut: 5.71 mg/100g, Soybean: 6.63 mg/100g, Cowpea: 6.63 mg/100g), and phytate (Groundnut: 160.89 mg/100g, Soybean: 30.63 mg/100g, Cowpea: 21.22 mg/100g). Vitamin analysis revealed variations in vitamin C (Groundnut: 57.94 mg/100g, Soybean: 58.31 mg/100g, Cowpea: 63.17 mg/100g) and β-carotene (Groundnut: 9.73 mg/100g, Soybean: 9.46 mg/100g, Cowpea: 7.51 mg/100g), indicating nutritional attributes of the studied leguminous forages. Based on the results, the treatment involving poultry dropping, goat dropping, pig dung and control all demonstrated good performance in terms of contributing to the nutritional composition of cowpea, groundnut and soybean. Thus, the use of poultry dropping and pig dung as organic manure for farmers in the region could be recommended.

EFFECT OF PROCESSING METHODS ON NUTRIENT ANDANTINUTRIENT COMPOSITION OF JUSTICIA SECUNDA LEAVES

Justicia secunda as a medicinal plant belongs to the family of Acanthaceae ,the leaf is an under-utilized medicinal vegetable that is believed and utilized as blood tonic by the local people and especially among the sect of some religious people that refuse blood infusion in medical conditions. This study examined the effect of processing methods on the nutrient and phytochemical composition of justicia secunda leaves. The leaves were subjected to blanching pre-treatment before sun drying for 72 hrs oven drying at 55 0C for 48 hrs and air drying for 120 hrsThe proximate, vitamins, minerals and phytochemical analysis were conducted on the samples using standard procedures. The results varied significantly (p&lt;0.05) in all the parameters measured according to the different processing treatments. Proximate composition of the samples ranged for crude protein (18.20-21.73%), crude fat (7.80-9.10%), crude fibre (12.00-14.90%), ash (15.50-17.40%), moisture (8.00-9.00%) and carbohydrate (27.98-35.50%). Mineral composition ranged for calcium (18.20-34.10 mg/100g), magnesium (47.11-54.29 mg/100g), sodium (126.43-136.47 mg/100g), potassium (425.49-453.03 mg/100g), phosphorus (135.40-145.76 mg/100g) and iron (33.07-38.94 mg/100g). Vitamin composition showed that thiamine (B1) ranged from 0.04-0.31 mg/100g, riboflavin (B2) (0.21-1.7 8mg/100g), niacin (B3) (0.49-1.89 mg/100g), vitamin C (6.31-19.48 mg/100g), while vitamin E ranged from (1.97-6.78 mg/100g). Anti-nutrient contents ranged for tannin (4.82-16.89 mg/100g), phenol (7.41-19.23 mg/100g), alkaloids (2.05-7.07 mg/100g) flavonoids (4.90-9.85 mg/100g) and saponin (3.25-8.35 mg/100g. Overall, the results revealed that air drying methods retained greater concentrations of fibre, minerals, vitamins and anti-nutrients than other processing methods. The high micro-nutrient contents of iron, anti-nutrient compounds and B group of vitamins in this processed vegetable reveals why it is used intuitively by the traditional people to boost health conditions due to the functional roles of iron, phytonutrients and B group of vitamins in heamopoisis in preventing morbidity and mortality especially among children.

Development and Formulation of Millet based Nutri-Bar

Whole grains like millet and rice are rich in nutrients and fiber, making them an excellent ingredient for developing a nutritious snack bar. The nutri bar was formulated using popped pearl millet and black rice flakes as primary ingredient. Three samples (F1, F2 and F3) with different composition are prepared. The proximate composition, chemical and sensory analysis was determined for all the samples. Sensory evaluation was conducted using a nine-point hedonic scale to assess the bar's taste, colour, texture, and overall acceptability. Results showed that the sample F3 has rich content of 12.8 gm of protein, 77.6 gm of carbohydrate, 366 Kcal of calories, 9.4 gm of dietary fiber were recorded per 100 grams of sample. Hence sample F3 is considered as the perfect formulation as it scored 8.5/9 in hedonic scale. Keywords: popped pearl millet, black rice flakes, sensory, nutrition, snack bar.

Proximate, elemental and anti-nutritional composition of &lt;i&gt;Pachycarpus bisacculatus&lt;/i&gt; (bitter butter) roots used locally as anti-snake venom

Proximate, elemental and anti-nutritional composition of &lt;i&gt;Pachycarpus bisacculatus&lt;/i&gt; (bitter butter) roots used locally as anti-snake venom

Edible Paper Sheets from Alternanthera philoxeroides and Hypophthalmichthys molitrix: Smart Biomass Valorization

Alternanthera philoxeroides and Hypophthalmichthys molitrix offer significant nutritional benefits. This study evaluates the proximate composition, amino acid profile, GC-MS analysis, FT-IR spectroscopy, SEM and EDX, and color values of edible paper sheets (EPSs) derived from Alternanthera philoxeroides incorporating different levels of Hypophthalmichthys molitrix flesh. The protein content in the EPSs varied based on fish flesh incorporation, peaking at 52.66% in Ap100/Hm300 (Non-boil). Protein and carbohydrate contents showed an inverse correlation across EPSs, with the highest carbohydrate content of 60.89% in sample Ap400/Hm0 (Boil). Lipid content was also found to correlate with H. molitrix flesh content in EPSs, ranging from 1.59% to 18.41%. Amino acid analysis identified 11 types, with methionine as the most prevalent, followed by leucine, phenylalanine, and lysine. GC-MS analysis revealed 51 bioactive compounds, including carbonic acid, hentriacontane, and various fatty acids. FT-IR analysis showed characteristic bonds, while color analysis displayed L* values ranging from 24.37 to 30.97. SEM analyses depicted the microstructure, surface view, and elemental composition of the EPSs, and EDX showed an abundance of Ca, N, K, O, C, Mg, Na, P, Cl, Mn, and Fe. Therefore, EPSs prepared from A. philoxeroides and H. molitrix could offer a promising approach for effectively utilizing aquatic biomass and providing both plant and animal nutrients to consumers.

Evaluation of lauric acid enhancement of black soldier fly larvae from coconut.

The current study evaluated the potential enhancement of lauric acid (LA) in black soldier fly, Hermetia illucens, (L.) (Diptera: Stratiomyidae) larvae (BSFL), a source of this short-chain fatty acid which has antimicrobial and immunostimulatory properties. Replicate groups of BSFL were reared on either the coconut or Gainesville diet for 7 days. After the rearing period, BSFL were harvested, purged, dried, and subjected to proximate, fatty acid and amino acid compositions, and pepsin digestibility analyses. Results demonstrate changes in proximate composition. BSFL reared on the coconut had significantly (P = 0.002) higher lipid content (47.3% vs. 25.2%) on a dry-matter basis. The LA concentration in BSFL produced on the coconut was 31% greater than those reared on Gainesville, resulting in almost 150% more LA. Furthermore, BSFL-fed coconut had reduced crude protein (29.7% of dry weight) and ash (3.7% of dry weight) relative to those fed Gainesville (43.4% and 7.5% for crude protein and ash, respectively) but higher pepsin digestibility (91.0% vs. 87.0%). The relative amounts of various amino acids in the 2 BSFL meals did not differ extensively, with statistically lower concentrations of only phenylalanine and tryptophan and higher concentrations of alanine, arginine, isoleucine, leucine, and serine in BSFL reared on coconut. Results demonstrate that the nutritional composition of BSFL can be manipulated, and an enhancement of LA concentrations of 150% was achieved with coconut, which has value for BSFL as a feed for various livestock, including aquaculture. Lower protein content is a tradeoff in terms of BSFL value as a feed additive.

Physicochemical properties, the digestible and resistant starch content of chestnut flour with different particle sizes

Physicochemical properties, the digestible and resistant starch content of chestnut flour with different particle sizes

Nutritional, Phytochemical and Antioxidant Characterizations of Wild Food and Medicinal Fruits Indigenous to Sudan: Sarcocephalus latifolius and Vitex doniana

ABSTRACT Sudan is a rich country in wild plants that play significant roles in securing food and medicine in rural areas, especially. This study explored Sarcocephalus latifolius (African peach) and Vitex doniana (black plum) for their fruits’ proximate composition, minerals, total soluble phenols, phenolic acids, total carotenoids, lutein, β–carotene, α–tocopherol, δ–tocopherol and total antioxidant capacity. African peach fruit and black plum pulp showed total soluble phenols amounting to 16.89 mg gallic acid equivalents (GAE)/g of Dry Weight (DW) and 7.56 mg GAE/g DW, respectively. The paramount phenolic acids were cinnamic acid (895 mg/kg DW) in African peach fruit and protocatechuic acid (1200 mg/kg DW) in black plum pulp. Total antioxidant capacities reached 268 µmoles trolox equivalents (TE)/g DW in African peach and 214 µmoles TE/g DW in black plum, when measured from hydrophilic extracts (HPE) using 2, 2-DiPhenyl-1-PicrylHydrazyl (DPPH) assay. The contents of total carotenoid were 47 mg/kg DW and 22.6 mg/kg DW, lutein reached 18.50 mg/kg DW and 15 mg/kg DW whereas β–carotene showed 0.31 mg/kg DW and 0.23 mg/kg DW in African peach and black plum, respectively. African peach showed α–tocopherol (167 mg/kg DW) whereas black plum showed δ–tocopherol (398 mg/kg DW). From this study, we concluded that African peach fruit and black plum pulp could be good sources of nutrition and antioxidants and have high potential for human health.

Hemp Flour as a Functional Ingredient for the Partial Replacement of Nitrites in a Minced Meat Model: Effect on Nutrient Composition, Antioxidant Profile and Sensory Characteristics

Consumers are becoming increasingly concerned about synthetic preservatives like nitrites in meat, prompting the meat industry to explore alternatives in order to lower nitrite levels. This study investigated the effects of incorporating hemp flour on the chemical and shelf-life characteristics of minced meat products with reduced nitrite content. Three types of products were prepared: HF0 (control) (0% hemp flour, 30 mg/kg NaNO2), HF4 (4% hemp flour, 15 mg/kg NaNO2), and HF6 (6% hemp flour, 15 mg/kg NaNO2). Analyses were conducted on proximate composition, fatty acid composition, antioxidant properties, lipid oxidation, colour, texture, and sensory characteristics. The addition of hemp flour at 6% reduced moisture content and influenced ash and sodium chloride levels in minced meat products. Despite the favorable fatty acid profile of hemp flour, its inclusion did not significantly alter the composition of the products. However, it did lead to significantly lower levels of lipid oxidation and modified the antioxidant capacity. Colour attributes were affected, with a higher hemp flour content resulting in colour deterioration. Cooking loss increased with a higher hemp flour content, and the minced meat products were significantly harder. Visual and olfactory sensory evaluation indicated that there were no significant differences in most traits, suggesting consumer acceptance of hemp-flour-enriched minced meat products. Overall, this study highlights the potential of hemp as a functional ingredient in minced meat products, also exhibiting the ability to reduce lipid oxidation.

Provenance and seasonal variations in proximate and cell wall composition of Melia dubia leaf fodder from Satpura and Sahyadri mountain ranges, India

Provenance and seasonal variations in proximate and cell wall composition of Melia dubia leaf fodder from Satpura and Sahyadri mountain ranges, India

Fermentative profile, nutritional composition, and aerobic stability of elephant grass (Pennisetum purpureum Schum) and forage peanut (Arachis pintoi) mixed silages

Objective. Determine the fermentative profile, proximate composition, and aerobic stability of mixed silages of elephant grass combined with levels of forage peanut. Materials and methods. Different levels of forage peanut (0.0, 20.0, 40.0, 60.0, and 80.0% on FM basis) were added to elephant grass silages. A completely randomized design was adopted, with 5 treatments and 3 repetitions, totaling 15 experimental silos that were opened after 30 days of sealing. Fermentative profile, proximate composition, and aerobic stability were evaluated. Results. The increase in the forage peanut levels in the elephant grass silages promoted a increasing on porosity, permeability, density, and pH (p&lt;0.001). A 0.58 reduction in Flieg index for every 1% forage peanut added to the elephant grass silage was observed (p&lt;0.001). The sum of the silage temperature difference compared to the environment (p=0.032) and aerobic stability (p&lt;0.001) showed a quadratic effect. The forage peanut inclusion in elephant grass silages reduced the dry matter, organic matter, neutral and acid detergent fiber, hemicellulose, cellulose, and total carbohydrates (p&lt;0.05) and increased the mineral matter, crude protein, lignin, non-fibrous carbohydrates, and total digestible nutrients (p&lt;0.05). Conclusions. Under the experimental conditions, recommend the inclusion of up to 40% forage peanut combined with elephant grass to compose mixed silages, due to the better fermentative dynamic, nutritional profile, and aerobic stability.

Comparative analysis of the chemical quality of fishmeal produced on the Northwest coast of Mexico

Objective: To evaluate the physico-chemical quality of fishmeal produced by four companies in different states of the Republic (Baja California Sur, Jalisco and Sinaloa). Design/methodology/approach: The analyzed fishmeals were from six batches, sardine meal from California pilchard and Pacific thread herring (S. sagax and O. libertate), and skipjack tuna and (K. pelamis) processed by different Mexican companies. Proximal chemical analysis was carried out at the Centro de Investigaciones Biológicas del Noroeste (CIBNOR). Results: The fishmeal’s quality parameters analyzed in this study showed similar values to those reported in the literature. The variations observed in their proximate chemical composition allow them to be classified according to the results of the analyses. Limitations on study/implications: Considering that four of the six flours were produced from the same raw material, S. sagax, the high variability in their physico-chemical quality parameters indicates a lack of standardization in both production methods and quality controls among the producing companies. Findings/conclusions: K. pelamis by-products can produce meals of equal or better physico-chemical quality than those produced from S. sagax. The development of official regulations establishing quality standards to fishmeal production at national level is desirable for competitiveness.

An approach to optimizing dietary protein to growth and body composition in walking catfish, Clarias batrachus (Linneaeus, 1758).

Clarias batrachus is a commercially important food fish. In the present study, effect of varying dietary protein levels was evaluated on the survival, growth parameters and proximate composition of C. batrachus. Diets comprising 25%, 30%, 35%, 40%, 45%, and 50% crude protein (CP) were supplied to fish in T1, T2, T3, T4, T5, and T6, respectively, at the rate of 5% of fish body weight for the entire 90 days, twice daily. Size of each stocked C. batrachus was recorded after 15 days. Results revealed 100% survival rate of C. batrachus in all treatments. Significantly highest (P<0.001) mean value of weight gain (g/fish), percent weight gain, daily growth rate, specific growth rate and protein efficiency ratio (PER) in C. batrachus were recorded, reared in T4 by feeding 40% CP in diet. The best FCR value (1.90±0.02) for C. batrachus was obtained in T4 by feeding 40%CP in diet. Mean value of water, ash, fat and protein contents (wet mass) were ranged 74.10-79.23%, 3.12-4.68%, 3.90-4.43% and 13.09-16.79% for C. batrachus in the studied treatment groups. Water content (%) was found significantly (P<0.05) higher in the body of C. batrachus for T1, T2, T3 and T6 than for T4 and T5. Ash was found significantly (P<0.05) higher in the fish reared in T4 and T5. Fat content in the wet body mass of C. batrachus was found significantly higher in T4 and T1. While, significant higher (P<0.05) values of mean protein content was noted in C. batrachus reared in T4 and T5. Body composition of C. batrachus was also categorically affected by body size, however, condition factor showed non-significant correlation in most of the relationships in the present study. Overall, results indicated that feeding appropriate diet (containing 40% CP) to the fish resulted good growth performance, lower FCR and higher protein content in the fish. Present study provides valuable knowledge of optimal dietary protein level in C. batrachus which will help in commercial success of aquaculture.

Quality of black pellets of charcoal fines produced with different moisture content and drying time

Charcoal fines are co-products of the wood carbonization process, unwanted because they reduce the gravimetric yield of the main product, charcoal. It can be produced due to the carbonization process, during the storage and transport of charcoal. Pelletizing charcoal fines increases the energy density of this material, producing a homogeneous fuel with lower moisture content, enabling burning in equipment with high energy efficiency, in addition to desirable properties in energy products such as high fixed carbon content and low moisture content. This study evaluated the effect of moisture content and drying time on the production of black pellets from charcoal fines bound with rice starch (Oryza sativa) on the physicochemical and mechanical characteristics. Black pellets were produced using two proportions of water (10 or 20%) and a drying time of 4 or 6 hours. Black pellets were evaluated for their proximate analysis, elemental composition, moisture content, size, apparent density, calorific value and energy density. The addition of water did not affect the content of volatile matter, ash, and fixed carbon, while the treatment with addition of 20% water and four hours of drying showed a higher moisture content (3.34%). The addition of water allowed for better heat conduction and particle arrangement, producing pellets with a durability greater than 98%. The better arrangement promoted by the addition of 20% water also increased the density of the pellets, resulting in greater energy density. Black pellets can be considered an alternative for residential heating, with T3 treatment (20% - 4h) with a high energy density and better performance in terms of desirable properties for use and commercialization according to the standard EN 14961-6.