Provisional Tolerable Weekly Intake Research Articles

An assessment of the potential health hazards associated with metal contamination in a variety of consumable species living along the industrialized coastline of East Java, Indonesia

The massive industrial growth in Gresik, East Java, Indonesia has the potential to result in metal contamination in the nearby coastal waters. The purpose of this study was to analyze the metal concentrations in edible species from the Gresik coastal waters and evaluate the potential health risks linked to this metal contamination. Metal concentrations (Cu, Fe, Pb, Zn, As, Cd, Ni, Hg, and Cr) in fish and shrimp samples mostly met the maximum limits established by national and international regulatory organizations. The concentrations of As in Scatophagus argus exceed both the permissible limit established by Indonesia and the provisional tolerable weekly intake (PTWI). The As concentration in Arius bilineatus is equal to the PTWI. The target cancer risk (TCR) values for both As and Cr in all analyzed species exceed the threshold of 0.0001, suggesting that these two metals possess the potential to provide a cancer risk to humans.

Total mercury in three small tunas from southeastern Brazil: Stable isotope relations and human risk assessment

Mercury (Hg) bioaccumulation can be highly variable in marine species. Its concentration is influenced by several physico-chemical and biotic processes, including size, age, diet composition and foraging habitats. Herein, we addressed total mercury (THg) concentrations in three small tuna species in Brazil: the frigate tuna Auxis thazard, the little tunny Euthynnus alletteratus and the skipjack tuna Katsuwonus pelamis. Samples were collected from commercial landings in the SE Brazilian coast from 2017 to 2019. The influence of trophic ecology and habitat preference on Hg accumulation was assessed through stable isotope [carbon (δ13C) and nitrogen (δ15N)] measurements. Additionally, the human health risk regarding the species consumption was assessed by the estimated weekly intake (EWI) and the provisional tolerable weekly intake (PTWI) for total consumers, adult men and adult women. Variables such as size and age were positively correlated to Hg, due to the bioaccumulation process. Trophic position appears to positively influence the mercury concentrations for A. thazard and K. pelamis, since relationship between δ15N and THg was significant. For E. alletteratus, the lack of relationship suggests that δ15N values do not vary much among adult fish (> 300 mm). Muscle THg concentrations in the three species did not exceed the established tolerable limit for mercury by Brazilian and international organs, as indicated by the %PTWI. This finding suggests that the consumption of those species caught off southeast Brazil at the considered amounts does not present a risk for healthy adults.

Evaluation of biotoxins and toxic metal risks in mussels from the Sea of Marmara following marine mucilage

The mucilage phenomenon observed in the Sea of Marmara in 2021, has raised public concern about seafood safety. Mediterranean mussels serve as a vehicle in food chain, enabling the transfer of pollutants. Farmed and wild mussels were collected from 4 different stations throughout the fishing season. Biotoxins causing amnesic, paralytic, or diarrhetic shellfish poisonings (ASP, PSP, or DSP) were examined during monthly samplings. Potential health risks posed by cadmium, lead and arsenic were assessed. Health risks were evaluated considering 150 g/week mussel consumption, accounting for the different age groups of consumers (50, 60, 70 kg). Estimated Weekly Intake calculations of metals were determined to be lower than Provisional Tolerable Weekly Intake at all age groups throughout the sampling period in all stations. Target Hazard QuotientCd of mussels captured from Istanbul Strait was always determined <1, while it was equal to 1 for 50 kg individuals in Gelibolu samples. All THQAs were >1. Target carcinogenic Risk was evaluated for Pb and iAs, which were found to be negligible and acceptable, respectively. No biotoxins responsible for ASP, PSP, or DSP were detected. Hg levels were under detectable limits. Excluding Cd, the results did not reveal any risks associated with mussel consumption during mucilage.

Chemical characterization of baby food consumed in Italy.

In this study, a total of 30 elements (essential and non-essential or toxic) were determined in 25 foods consumed in Italy by children aged 0-6 months and produced in Europe. Inductively Coupled Plasma-Atomic Emission Spectrometry and Inductively Coupled Plasma-Mass Spectrometry were used as measurement techniques for the elements of interest. The estimated intakes for one-year-old infants were compared with risk estimators and nutritional requirements. Data indicate that commercially available baby food in Italy provides an excellent contribution for Mn, Cu, Fe, Zn, Ca, K, and P, covering up to approximately 70% of the adequate intake (AI) for an infant aged 6-12 months. The intake of detectable toxic elements was always below the safety limit: even the most concentrated toxic elements never exceeded about 86% of the Provisional Tolerable Weekly Intake (PTWI). This result indicates that the analyzed baby food is of good quality and does not pose risks to children's health.

Evaluation and risk assessment of heavy metals in King tuber mushroom in the contest of COVID-19 pandemic lockdown in Sokoto state, Nigeria

The aim of this study was to evaluate the degree of contamination and intake rates, as well as the risk of Lithium (Li), Arsenic (As), Cadmium (Cd), Mercury (Hg), and Lead (Pb) contained in wild species of King Tuber Mushroom (P. tuber-regium) collected pre-, post- and during the COVID-19 lockdown which is widely consumed in northwestern Nigeria; and to also assess their potential to accumulate some heavy metals, which comprises the linked consumer health hazards by assessing the risks to children, adolescents, and adults from cancer-causing and non-cancer-causing substances based on estimated weekly intake (EWI). After mineralization with acid, the concentrations were determined using Optical Emission Spectrometry with Inductively Coupled Plasma (OES-ICP), and Mercury (Hg) using Cold Vapor Atomic Fluorescence Spectrophotometer (CV-AFS). Moreover, the health risk assessment due to the ingestion of these elements through the consumption of P. tuberregium was estimated. The findings showed that concentrations of As, Pb, Cd, Li and Hg varied greatly within between 2018 and 2022, with levels higher in pre- and post- COVID-19, and lower during the COVID-19 lockdown. In descending order, the average content of these substrates and metals, was As > Pb > Cd > Li > Hg. Children, adolescents, and adults all had EWI values below the mushroom's provisional tolerable weekly intake (PTWIs). The consumption of P. tuber-regium harvested from the areas investigated, therefore, poses no toxicological or health risks to the inhabitants.

Non-carcinogenic human risk assessment of the consumption of infant formulas contaminated with total and organic mercury

The quality of food is paramount for the healthy growth and development of children, with nutrients and contaminants like mercury playing significant roles here. In this study, the levels of total mercury (THg) and methylmercury (MeHg) in infant formulas such as the first infant formula, hypoallergenic formula, comfort formula against colic and constipation, and anti-reflux formula were investigated. A validated and optimized cold atomic absorption spectrometry method of direct thermal decomposition with gold amalgamation was used. Analysis revealed notable variations in THg and MeHg levels among products. Basic infant formulas were characterized by the highest average and median THg values (6.14 µg/kg, and 3.27 µg/kg, respectively), while hypoallergenic preparations showed the lowest THg content (1.069 µg/kg on average and 0.922 µg/kg for the median). Methylmercury levels were the lowest in preparations meant to treat constipation and colic, whereas anti-reflux formulas were characterized by the highest mean and median values. Based on the evaluation of the Provisional Tolerable Weekly Intake realization for THg, and MeHg, it was determined that the mercury content in infant formulas was within acceptable consumption limits. However, the target hazard quotient, and the total hazard index revealed elevated mercury levels in certain products, potentially posing health risks.

Metal levels of canned fish sold in Türkiye: health risk assessment.

This study analyzed 34 canned fish products, including 28 tuna specimens, 3 salmon specimens, 1 mackerel specimen, and 1 anchovy specimen, from 13 different brands purchased in Türkiye. The study aimed to determine metal/metalloid levels in canned fish and potential health risks for both children and adult consumers. The metal/metalloid levels in the samples were determined using an Inductively Coupled Plasma-Mass Spectrometer (ICP-MS), with the range of levels found as follows (mg/kg, ww): Fe (12.12-101.4), Cu (2.19-11.68), Zn (4.06-33.56), Se (0.24-10.74), Al (1.41-14.45), Cr (0.06-4.08), Pb (0.10-0.43), Cd (0.001-0.110), and As (0.01-0.13). Estimated weekly intake (EWI) levels were found that the consumption of canned fish products did not pose any risk based on the EWI levels and provisional tolerable weekly intake (PTWI) limits. However, three tuna samples had target hazard quotient (THQ) levels above the threshold (>1). Arsenic levels were found to increase the carcinogenic risk for child consumers if they heavily consumed 18 canned fish products, including 15 tuna, 2 salmon, and 1 mackerel. The maximum allowable consumption rates (CRmm) for each canned fish product were calculated monthly. Consequently, the consumption of canned fish by children can pose health risks.

Assessment of Exposure to Aluminum through Consumption of Noodle Products.

This study aimed to determine aluminum (Al) contents in commonly consumed noodles and estimate the risk of Al exposure through the consumption of noodles in the Thai population. A total of 80 samples, 20 samples each of rice stick noodles, egg noodles, wide rice noodles, and Thai rice noodles was purchased from markets in Bangkok Metropolitan and other four provinces in each region of Thailand. Wet digestion and graphite furnace atomic absorption spectrometry (GFAAS) were used to determine Al contents. Exposure assessment of Al was conducted by applying the consumption amounts of noodles from the national consumption survey and the Al contents of the noodle samples. The hazard quotient (HQ) was calculated to estimate the risk of exposure to Al. Aluminum contents in the noodles ranged from not detected to 630 mg/kg. Exposure to Al from consumption of each noodle product in all age groups was lower than the provisional tolerable weekly intake (PTWI). However, Al exposures in children calculated from the high consumer models and Al exposures in all age groups from the worst-case scenarios were higher than the PTWI, indicating potential adverse health effects. Consumers who regularly consume large amounts of noodle products containing Al may be at risk of having adverse health effects.

Assessment of health risks due to toxic metals in demersal fish captured from Saros and Edremit Bays, Northern Aegean Sea.

Bays are vulnerable ecosystems generally located near densely populated areas where toxic metals tend to accumulate and stay longer, affecting marine life. This study aimed to investigate the age-based health risks arising from Hg, Cd, Pb, and As in demersal fish captured from two major bays in the Aegean Sea. For this purpose, red mullet, whiting, piper gurnard, and tub gurnard, frequently consumed species, were caught from Saros and Edremit Bays. Toxic metal concentrations were determined from the muscle tissue of fish. Health risk assessments were conducted by the estimation of weekly intake (EWI), provisional tolerable weekly intake (PTWI), target hazard quotient (THQ), total THQ (TTHQ), and target carcinogenic risk (TR). Red mullet from Edremit Bay was the species with the highest toxic metal levels, which were 1.597mg/kg, 0.041mg/kg, 0.070mg/kg, and 19.351mg/kg for Hg, Cd, Pb, and As, respectively. Whiting from Edremit Bay had higher mean concentrations of Hg and As than those from Saros Bay. The levels of Hg, Pb, and As (0.328, 0.043, and 0.574mg/kg) in the tub gurnard were higher in comparison with the piper gurnard (0.252, 0.020, and 0.382mg/kg) caught in the same station in Saros. TTHQs of red mullet and whiting from the same bay were found to be > 1, indicating potential health risks for all nine age categories studied. On the other hand, TTHQs of all species from Saros Bay were determined to be > 1 for the first four age categories, which might trigger health risks for children and adolescents. According to the TR index for Pb, no risk was determined for the fish from both bays. However, TR calculations for inorganic As indicated high cancer risk in most of the age categories for red mullet and whiting from Edremit Bay. To sum up, the results revealed that the fish captured from Edremit Bay posed serious health risks in terms of Hg and As concentrations for all nine age categories. Surveillance and monitoring of toxic metal levels in demersal fish and population-based health risk evaluation are vital in heavily populated bays.

Assessing heavy metal levels in pediatric enteral nutrition formulas available in the Turkish market: Implications for consumer health.

In this study, we assessed the levels of lead (Pb), cadmium (Cd), mercury (Hg), and inorganic arsenic (iAs) in 27 pediatric enteral nutrition (EN) formulas from five international brands available in the Turkish market. Analysis was conducted using inductively coupled plasma mass spectrometry (ICP-MS). Non-carcinogenic and carcinogenic risk assessment was performed using hazard quotient (HQ), hazard index (HI), carcinogenic risk (CR), Toxicological contribution % of Provisional Tolerable Weekly Intake (PTWI) models. Our objective was to evaluate heavy metal exposure in EN formulas, specifically focusing on Cd, Pb, iAs, and Hg levels according to recommended amounts for different age groups based on their energy requirements. Average concentrations of iAs in polymeric (PC), oligomeric (OC), and monomeric (MC) EN formulas were as follows: PC: 2.13±0.16 (<LOD-13.86) μg/kg, OC: 4.29±0.10 (0.38-11.98) μg/kg, MC: 8.62±0.19 (1.60-27.20) μg/kg. For Cd levels, average concentrations in PC, OC, and MC formulas were: PC: 0.57±0.03 (<LOD-3.09) μg/kg, OC: 1.31±0.07 (0.40-3.12) μg/kg, MC: 0.93±0.07 (0.07-2.33) μg/kg. Similarly, average concentrations of Hg in PC, OC, and MC formulas were: PC: 0.13±0.01 (<LOD-0.14) μg/kg, OC: 0.18±0.01 (<LOD-0.18) μg/kg, MC: 0.20±0.02 (<LOD-0.34) μg/kg. Lastly, average concentrations of Pb in PC, OC, and MC formulas were: PC: 2.32±0.06 (1.52-2.96) μg/kg, OC: 0.97±0.06 (0.37-2.26) μg/kg, MC: 2.58±0.08 (1.95-3.28) μg/kg. The exposure levels of Cd, Pb, iAs, and Hg calculated in this study did not exceed the PTWI threshold values established by the European Food Safety Authority (EFSA). Average HQ values for each heavy metal exposure in males and females were below 1. However, P95 values for iAs exceeded 1.00 in all age groups. The HI value was greater than 1.00 in all age groups and genders. The presence of heavy metals in EN formulas may pose health risks, particularly for sensitive individuals.

Risk assessment of trace metals in canned fish commercialized for human consumption in Egypt

Fourteen trace elements were examined using ICP-OES in the imported canned tuna fish, sardines, and tuna packaging medium that were sold in Greater Cairo. The leaching into acetic acid (3%) was done to simulate the releasing of trace metals from cans. The association between the trace metal levels in tuna, oil/brine, and the leaching of trace metals into 3% acetic acid solution was investigated. All trace metals were found in all samples, although Cd was not. Eight of 25 canned tuna (32%), and one of 5 canned Sardina pilchardus fish (20%) have Pb concentrations that exceeded the permissible limit (PL), whereas one albacore tuna (1/25; 4%) exceeded Finland's PL for As. Except for aluminum, which had higher concentration in oil/brine than in brine alone, there were no variations in other metal concentrations. Although, there is no considerable release of most metals into acetic acid, Fe and Al did, with a leaching median of 32 and 0.16 mg/L, respectively. The average daily intake of arsenic (0.850 mg) was greater than tolerable daily intake (TDI) for adults advised by WHO. The provisional tolerable weekly intake (PTWI) for As (0.085 mg/kg bw/week) also surpassed the advised PTWI. Only 3 out of 30 samples (10%) had PTWIs for Pb above them. The computed non-carcinogenic hazard index (HI) indicating that canned tuna consumption may have health risk for human. The most significant factor in all samples' higher target hazard quotient (THQ) levels was arsenic. This study comprises popular tuna fish species from Thailand, Vietnam, Italy, and Morocco, including skipjack, longtail, albacore, and sardina fish. Thus, it is pertinent to all nations that import tuna from these nations.

Mercury in honey from the Marche region (central Italy). Risk assessment from human consumption and its use as bioindicator of environmental pollution

Honey is a natural product made by honeybees (Apis mellifera) from nectar or honeydew. It is a very popular and appreciated product all over the world as it represents a rapidly available energy source and exerts several beneficial properties for humans. However, it has been demonstrated that honey can be contaminated by potentially toxic elements (PTEs) of natural or anthropogenic origin. Among them, mercury (Hg) represents one of the most dangerous for its toxicity and its capacity to biomagnify along the trophic web. In the present study, 100 honey samples from the Marche Region (Central Italy) produced in the year 2021, were analyzed by thermal decomposition amalgamation atomic absorption spectrometry to determine the Hg content. The overall mean concentration was 0.2 ± 0.2 μg kg−1. The results showed that no statistically significant differences were found in Hg content among honey from different pollen origin, but honeydew had a significantly higher Hg content with respect to all other honey samples (0.6 ± 0.3 μg kg−1). The Hg content in honey depends mainly on local pollution, while geographical origin did not play a key role. Furthermore, considering the regulatory limits and provisional tolerable weekly intake (PTWIs) identified by FAO/WHO, the Hg Hazard Quotient (HQ) measurement revealed that this product is safe for human consumption.

Mercury and selenium concentrations and their toxicological implications in silky sharks Carcharhinus falciformis (Elasmobranchii: Chondrichthyes) in the northwestern Indian Ocean

Mercury (Hg) and selenium (Se) were assessed in silky sharks, Carcharhinus falciformis, a carnivorous apex predator and an important resource in the Indian Ocean. Muscle from the caudal peduncle was collected from individual sharks and analyzed using ICP-MS. Results showed that 40% of the specimens exceeded the allowed limits (1.00μg/g ww) of Hg. The mean Hg concentration (1.39 ±1.44μg/g ww) was also higher than the limits. The mean Se concentration in shark muscle was 0.324 ±1.44μg/g ww. The mean Se/Hg molar ratio was 2.23 (range: 0.17–24.5), with 30% of individuals exceeding 1.0, showing a protective effect of Se against Hg. However, for individual sharks’ mean Se health benefit value (Se-HBV) was −3.83. The risks of consuming silky sharks by male and female adults were also calculated. Results showed that the estimated weekly intake (EWI) was 2.9 to 3.5 times higher than the provisional tolerable weekly intake (PTWI). Consumption risks based on hazard quotient (HQ), estimated daily intakes (EDI), and EWI exceeded the threshold for fish consumption by adults, posing immediate health concerns.

Investigating metal pollution in the food chain surrounding a lead-zinc mine (Northwestern Iran); an evaluation of health risks to humans and animals.

The current study aims to evaluate the health risk of heavy metals for humans and animals in the Angouran mining complex (northwest of Iran). Twenty-five plant species and their corresponding soils (natural soils) were collected along with mine tailings samples. The carcinogenic and non-carcinogenic risks of heavy metals (Zn, Pb, Cd, Cr, and Co) for humans using the hazard quotient (HQ) and hazard index (HI) were evaluated. Moreover, the health risk caused by forage feeding to grazing ruminants (cow and sheep) and the risk associated with animal products consumption by humans in the soil-plant-animal transfer system were assessed. The value of HI in natural soils (rangeland use) was less than one (HI < 1), while regarding tailings, the HQ via oral ingestion and the HI were greater than one (HI & HQ > 1). The range of total carcinogenesis risk in natural soils exceeded the target risk (Risk < 10-6) and for tailings, it showed the probability of cancer risk, 1 person per 3636 populations, which is much higher than the acceptable or tolerable range (10-4 < Risk < 10-6). Regarding the animal health risk, the content of Pb and Cd in most of the animal organs was higher than the control values. In turn, dietary exposure to Pb and Cd is worrying for residents due to exceeding the provisional tolerable weekly intake (PTWI). This comprehensive study suggests the necessity of risk assessment of mining sites in Iran and immediate control measures to diminish pollutants.

Evaluation of heavy metals contamination in cereals, vegetables and fruits with probabilistic health hazard in a highly polluted megacity.

Heavy metals (HMs) contamination in foodstuffs could pose serious health issues for public health and humans are continually exposed to HMs through the consumption of cereals, fruits, and vegetables. The present study was conducted to assess 11 HMs in foodstuffs to investigate pollution levels and health risks to children and adults. The mean contents of Cd, Cr, Cu, Ni, Zn, Fe, Pb, Co, As, Mn and Ba in foodstuffs were 0.69, 2.73, 10.56, 6.60, 14.50, 9.63, 2.75, 0.50, 0.94, 15.39 and 0.43mg/kg, respectively and the concentration of Cd, Cr, Cu, Ni and Pb were higher than maximum permissible concentrations (MPCs) showing that these foods may be contaminated with metals and constitute a danger to consumers. Vegetables had relatively higher metal contents followed by cereals and fruits. The average value of the Nemerrow composite pollution index (NCPI) for cereals, fruits, and vegetables were 3.99, 6.53, and 11.34, respectively indicating cereal and fruits were moderately contaminated whereas vegetables were heavily contaminated by the studied metals. The total estimated daily and weekly intakes for all studied metals were higher than the maximum tolerable daily intake (MTDI) and provisional tolerance weekly intake (PTWI) recommended by FAO/WHO. The target hazard quotients and hazard index of all studied metals exceeded the standard limit for adults and children suggesting significant non-carcinogenic health hazards. The total cancer risk value of Cd, Cr, Ni, Pb, and As from food intake exceeded the threshold range (1.0E-04), suggesting potential carcinogenic risks. Based on practical and sensible evaluation techniques, the current work will assist policymakers in controlling metal contamination in foodstuffs.

Toxicological risk in individuals exposed to methylmercury and total mercury through daily-consumed foodstuffs in one of the mining regions of Bajo Cauca, Antioquia, Colombia

Mercury (Hg), and its different chemical forms, such as methylmercury (MeHg), are highly toxic compounds that affect the environment as well as human and animal health. Specifically, mining practices may cause these metals to be present in water, air, soil, and food. This study examines the concentration levels of Hg and MeHg in locally-consumed foodstuffs, including fish, pork, chicken, yam, plantain, and cassava, in a specific region of Antioquia (Colombia) by assessing the toxicological risk to the population due to the exploitation of open-pit alluvial mining in the area. Among foods examined, the highest amount of Hg was found in fish > pork > chicken > cassava > plantain > yam (ranging from 207.87 to 0.23 μg/g). Conversely, MeHg was identified in cassava > fish > plantain > chicken > yam > pork in amounts ranging from 1.567 to 0.434 μg/g. The risk exposure factors of the acceptable daily intake (ADI) and the provisional tolerable weekly intake (PTWI) for Hg and MeHg exhibited a trend similar to the amount of metal in the assessed foods. This work contributed to characterizing and preventing public health problems in a mining population in the Bajo Cauca region of Antioquia, which requires special attention due to their potential exposure to Hg and MeHg through the most locally consumed foods.

The Toxicological Analysis and Toxicological Risk Assessment of Chosen Elemental Impurities (Ag, Au, Co, Cr, Cs, Li, Mo, Se, and Sr) in Green Tea (Camellia sinensis (L.)) Infusions

Our study's objective is to evaluate the potential health effects of elemental impurities (Ag, Au, Co, Cr, Cs, Li, Mo, Se, and Sr) found in green tea infusions (Camellia sinensis (L.)). The ICP-MS-based methodology was employed for elemental analysis and a detailed health risk evaluation based on weekly consumption (μg/L of infusion/week). The provisional tolerable weekly intake (PTWI), established by the Joint FAO Expert Committee on infusion/week/month based on existing literature data, was then compared to the subjects with data from the available literature. The exposure of the study items to Co ranged from 0.07904 to 0.85421 μg/day. On the contrary, the ICH (International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use) guidelines state that the established permitted daily exposure PDE (oral exposure) for Co is 50 μg/day. The PDE of lithium is around 560 μg/day, and according to our study, the estimated daily exposure of the evaluated products to Li fell between 0.0185 and 0.7170 μg/day. Our research also revealed modest concentrations of Cs (0.399-2.654 μg/L), Mo (0.0113-0.107 μg/L), and Sr (9.848-22.331 μg/L) in infusions. The recognized PDE for molybdenum is approximately 3400 μg/day. Only two samples contained silver, and when considering daily consumption, the predicted daily exposure to Ag is between 4.4994 and 0.03122 μg/day. The amounts of all evaluated elements in a daily dose of green tea infusions should not harm the consumer's health. Further considerations should take aspects, such as constant change and environmental pollution, into account.

Trace Elements in Soy-Based Beverages: A Comprehensive Study of Total Content and In Vitro Bioaccessibility.

Soy-based beverages are one of the most consumed plant-based beverages, which have been used as a substitute for dairy products. Soy is a source of several nutrients (vitamins, minerals, and phenolic compounds, etc.) and its consumption is usually associated with several benefits, such as the prevention of cardiovascular diseases, cancer, and osteoporosis. However, non-essential trace elements can be found in these beverages. Thus, a comprehensive study concerning trace elements Al, As, Cd, Co, Cr, Cu, Fe, Li, Mn, Ni, Pb, Sb, Se, Sn, Sr, and Zn in soy-based beverages was proposed. In vitro digestion allowed to simulate the gastrointestinal juice (bioaccessibility) and the Caco-2 cells culture model was applied for the bioavailability assay. Trace elements measures were performed by inductively coupled plasma optical emission spectrometry (ICP OES). Multivariate analysis classified soy-based beverages according to their soy source (isolate protein, hydrosoluble extract, and beans); Al, Cu, Fe, Mn, Sr, Se, and Zn bioaccessible fractions corresponded to approximately 40%-80% of their total content, and soy-based beverages were found to be a good Fe, Se, and Zn source. However, our results showed risk exposure assessment from daily consumption of one glass of soy-based beverage can contribute to 3.5% and 0.9% of Al Provisional Tolerable Weekly Intake (PTWI) for children and adults, respectively.

Heavy metal exposure risk associated with ingestion of Oreochromis niloticus and Coptodon kottae harvested from a lacustrine ecosystem

Lacustrine ecosystems have not been widely assessed for heavy metal contamination and associated health risks; yet, they could be accumulating these contaminants to the detriment of aquatic organisms and communities relying on them for various aspects. The water quality index (WQI) and concentrations of heavy metals including As, Cd, Co, Cu, Cr, Fe, Mn, Ni, Pb, and Zn in water, sediment, Oreochromis niloticus, and in the endemic and endangered Coptodon kottae in Lake Barombi Kotto in Cameroon were determined to evaluate fish heavy metal bioaccumulation, and heavy metal exposure risk posed to communities consuming these fish species. The WQI of the lake was found to be excellent with heavy metal concentrations that were lower than what was obtained in the sediments and fish samples. Mean heavy metal concentrations in sediment ranged from 0.86 ± 0.03 mg/kg for Cd to 560.1 ± 11.15 mg/kg for Fe. In both fish species, Fe, Mn, and Cu had the highest concentrations. Though the heavy metal concentrations in the lake water were low, heavy metal bioconcentration factors for both fish species were very high ranging from 1.6 for Fe to 1568 for Mn. The concentration patterns of heavy metals in the organs of both fish species followed the order bones > gut > muscle. Consumption of these two fish species contributes less than 1.0% of the permissible tolerable daily intake (PTDI) and provisional tolerable weekly intake (PTWI) of these metals with lead (Pb) having the potential to exceed permissible exposure levels when high amounts of these fish are consumed by adults.

Accumulation and distribution of mercury in agricultural soils, food crops and associated health risks: A case study of Shenda gold mine-Geita Tanzania

Contamination of the environment and foodstuff by potentially harmful elements (PHEs) has become a serious concern due to the associated health risks to the population. In the present study total mercury (Hg) levels in soil and food crops from farms around Shenda gold mine in Geita Tanzania was determined by Cold Vapor Atomic Fluorescence Spectrometry (CVAFS). Generally, it was found that there was significant different of Hg content in soil and food crops (P < 0.05) among studied sites. The total Hg concentrations determined were in the ranges of; soils (0.003-0.1220 mg/kg), rice (0.0752-0.1587 mg/kg) and vegetables (0.0556-0.3439 mg/kg) all measurement were based on dry weight basis (dw). Total Hg levels in soil were compared with the maximum allowable concentration (MAC) set by Tanzania Bureau of Standards (TBS) and United Kingdom (UK). All studied soil samples were within acceptable range (2 mg/kg) set by TBS and UK (1 mg/kg). Total Hg contents in food crop samples were compared with Chinese MAC of Hg (0. 01 and 0.02 mg/kg for leafy vegetables and grains respectively in a fresh weight basis). It was observed that Hg contents in potato leaves, pumpkin leaves and Chinese cabbage were within the MAC while Hg levels in cassava leaves and rice grains exceeded the MAC. In addition, Hg associated health risks to consumers of contaminated foods for residents around Shenda gold mine were estimated. The estimated weekly intake (EWI) of Hg due to consumption of rice grain was above the provisional tolerable weekly intake (PTWI) set by FAO/WHO (1.6 mg/kg bw/week) while the EWI due to consumption of leafy vegetables were below the PTWI. Target hazard quotient (THQ) was < 1 due to consumption of vegetables while THQ value for rice was >1, indicating a potential non-carcinogenic risk to adult population from the consumption of rice grain from the study area. Therefore, people living near Shenda goldmine might be potential victim of Hg accumulation in soil and food crops, thus necessary management options have to be in place.