Proteins In Blood Of Patients Research Articles

Association of albumin, fibrinogen, and modified proteins with acute coronary syndrome.

Acute coronary syndrome (ACS) comprises a pathophysiological spectrum of cardiovascular diseases related to atherosclerotic coronary plaque erosion. Oxidative stress and inflammation play pivotal roles in the development and progression of atherosclerosis, which affects circulatory proteins, including albumin and fibrinogen, thereby causing an imbalance in albumin to globulin and fibrinogen to albumin ratios. This study aimed to assess the effect of oxidative stress on circulatory proteins, correlate these parameters, and investigate their significance in patients with ACS. In this case-control study, the major blood proteins in patients with ACS and a control group were evaluated using standard methods. Out of 70 ACS cases, 75.7% had ST-elevation myocardial infarction (STEMI), 18.6% had non-STEMI, and 5.7% had unstable angina. The mean cardiac troponin I level in patients was 12.42 ng/mL. The patients demonstrated a significantly reduced level of human serum albumin (HSA), 3.81 ± 0.99 g/dL, compared to controls, 5.33 ± 0.66 g/dL. The albumin to globulin ratio (AGR) was significantly depressed in patients while their mean fibrinogen level and the fibrinogen to albumin ratio (FAR) were significantly higher. Multivariate logistic regression analysis showed that albumin and fibrinogen were significantly associated with the risk of ACS, showing the potential of these parameters to be used for risk assessment of ACS. The ischemia modified albumin (IMA) and protein carbonyls were significantly higher in patients which showed significant positive correlations with FAR. Albumin, IMA and protein carbonyls were found to have high diagnostic sensitivity and specificity for ACS. Overall, these circulatory and modified proteins in ACS patients, particularly lower HSA, AGR, and higher IMA and protein carbonyls may help assess risk.

Shared and differing inflammatory blood proteins in patients with hidradenitis suppurativa and psoriasis

British Journal of DermatologyVolume 186, Issue 4 p. e178-e178 Plain Language Summary Shared and differing inflammatory blood proteins in patients with hidradenitis suppurativa and psoriasis First published: 04 April 2022 https://doi.org/10.1111/bjd.21072AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Linked Article: Navrazhina et al. Br J Dermatol 2022; 186:684–693. Hidradenitis suppurativa (HS) and psoriasis are both inflammatory skin diseases that have a great negative impact on patients’ lives and affect about 2% of the population worldwide. This study, from the USA, set out to look at how multiple blood proteins related with various biological processes change as a result of these two diseases. We recruited patients with moderate-to-severe HS and patients with psoriasis as well as healthy volunteers and analysed their blood samples. Overall, we measured and compared the levels of 1536 different proteins across these three groups. We found that in patients with equally severe disease, the patients with HS had a greater disruption of protein levels (such as tumour necrosis factor and interleukin-1β), even though HS involves a much smaller area of the skin compared with psoriasis. We also identified proteins that were showing similar changes in their levels in both the patients with HS and those with psoriasis (for example, an increase in the inflammatory protein interleukin-17A, that is also a target for a few effective psoriasis treatments). Proteins related with inflammation and proteins related with atherosclerosis (which means they may also be linked with cardiovascular diseases) were connected with the severity of the disease in both patients with psoriasis and those with HS. Our findings present the similarities and differences in blood protein levels in patients with HS and patients with psoriasis and may help to guide the use of specific psoriasis treatments in patients with HS, as well as the development of new treatments, which are needed for patients with moderate-to-severe HS. Volume186, Issue4April 2022Pages e178-e178 RelatedInformation

Circulating Brain Injury Exosomal Proteins following Moderate-To-Severe Traumatic Brain Injury: Temporal Profile, Outcome Prediction and Therapy Implications.

Brain injury exosomal proteins are promising blood biomarker candidates in traumatic brain injury (TBI). A better understanding of their role in the diagnosis, characterization, and management of TBI is essential for upcoming clinical implementation. In the current investigation, we aimed to explore longitudinal trajectories of brain injury exosomal proteins in blood of patients with moderate-to-severe TBI, and to evaluate the relation with the free-circulating counterpart and patient imaging and clinical parameters. Exosomal levels of glial (glial fibrillary acidic protein (GFAP)) and neuronal/axonal (ubiquitin carboxy-terminal hydrolase L1 (UCH-L1), neurofilament light chain (NFL), and total-tau (t-tau)) proteins were measured in serum of 21 patients for up 5 days after injury using single molecule array (Simoa) technology. Group-based trajectory analysis was used to generate distinct temporal exosomal biomarker profiles. We found altered profiles of serum brain injury exosomal proteins following injury. The dynamics and levels of exosomal and related free-circulating markers, although correlated, showed differences. Patients with diffuse injury displayed higher acute exosomal NFL and GFAP concentrations in serum than those with focal lesions. Exosomal UCH-L1 profile characterized by acutely elevated values and a secondary steep rise was associated with early mortality (n = 2) with a sensitivity and specificity of 100%. Serum brain injury exosomal proteins yielded important diagnostic and prognostic information and represent a novel means to unveil underlying pathophysiology in patients with moderate-to-severe TBI. Our findings support their utility as potential tools to improve patient phenotyping in clinical practice and therapeutic trials.

P-27 - Oxidazed proteins in blood of patients with nephropathies of various origins

The main purpose of our study was to investigate the oxidative modifications of proteins in blood of patients with nephropathies of various origins. There were 80 patients divided into 4 groups depending on the type of nephropathy. 1st group was represented by patients with acute alcoholic nephropathy; the 2nd group - by patients with nephropathy caused by acute poisoning with alcohol surrogates; in the 3th group pregnant women with gestosis were included; the 4th group – was represented by patients with acute medication nephropathy (acute drug poisoning). The control group consisted of 15 healthy subjects. The reactive protein carbonyl derivatives were detected in plasma and erythrocytes (Levine et.al, 1990), advanced oxidation protein products (AOPP) were detected in serum (Witko-Sarsat et al., 1996). In blood plasma of patients of 1–4 groups the concentration of reactive protein carbonyl derivatives was lower in comparison with control ones (p

Clinical and biochemical aspects of coagulation control in patients with atrial fibrillation and excessive alcohol consumption

Aim. Reveal the influence of excessive alcohol consumption on blood coagulation system, levels of blood proteins in patients with atrial fibrillation treated with warfarin.
 Methods. The study included 80 patients with a diagnosis of atrial fibrillation, treated with vitamin K antagonists. The study group included 34 patients with excessive alcohol consumption (according to the questionnaire), the comparison group - 46 patients with no excessive alcohol consumption. Transferrin, hemopexin, aspartate transaminase, alanine transaminase, gamma-glutamyl transferase, carbohydrate-deficient transferrin levels were measured in venous blood serum, serum protein electrophoresis was performed. Parameters of plasma hemostasis tests and D-dimer level were also analyzed.
 Results. Gamma-glutamyl transferase activity in patients of study group was 1.5 times higher compared to the comparison group (p=0.021). There was no statically significant differences in aspartate transaminase and alanine transaminase activity, but their levels were increased in alcohol abusers with gamma-glutamyl transferase above normal values (55 U/l). None of the patients had increased level of carbohydrate-deficient transferrin. Absolute and relative serum β1-globulin concentrations were significantly reduced in study group patients, while hemopexin and transferrin levels were at the same level as in the control group. No differences of coagulation parameters were between the groups. In the study group, in patients with β1-globulin levels below the median values, international normalized ratio was significantly closer to the target values, but the same patients had higher D-dimer levels, which apparently indicates low effect of anticoagulants.
 Conclusion. The effect of anticoagulant was low in patients with excessive alcohol consumption and atrial fibrillation, as evidenced by elevated D-dimer levels at international normalized ratio close to the target values. Patients with atrial fibrillation and excessive alcohol consumption with elevated D-dimer levels had serum β1-globulin levels reduced compared to median values.

PP31 - Changing of extracellular nucleic acids and histone like proteins in blood of patients with complications of peptic ulcer disease

Peptic ulcer disease - a chronic relapsing poly etiologic disease is widespread throughout the world. Among the patients who are hospitalized with gastrointestinal diseases, peptic ulcer disease is diagnosed in 35.8% of cases. Implementation of peptic ulcer disease is carried out by the interaction of exogenous and endogenous factors: genetic predisposition, such as the nervous and endocrine systems, psycho-emotional characteristics, the characteristics of metabolic, biochemical reactions. In modern conditions, there is a noticeable increase in the incidence of peptic ulcer caused by stressful situations, bad habits, use of drugs, resulting in impaired balance between “aggressive” factors and “protective” factors of gastroduodenal zone. Aim of research Investigating of extracellular nucleic acid contamination changing and histone like proteins in blood of patients with complications of peptic ulcer disease. Material and methods The object of the study was the blood of patients with ulcer complications, which were determined precursors of nucleic acids and histone like proteins. Results of study A significant increase in the fraction 1.9 times H1, H2A, and H3, H4 fraction in 3.5 times in comparison with the control values and a significant decrease H2B fraction 1.5. The quantity of ASF in the blood of patients with peptic ulcer disease significantly increased by 1.2 times compared with the control, with corresponding reduction of circulating extracellular nucleic acids. Conclusion Changing the content of histone like proteins extracellular nucleic acids, in patients with a complication of gastric ulcer, can be explained by “breakdown” compensatory mechanisms adapted by the action of unfavorable factors that lead to oxidative degradation of histone proteins and metabolic disorders predecessors nucleic acids.

PP46 - Oxidized proteins in blood of patients with very severe chronic obstructive pulmonary disease

PP46 - Oxidized proteins in blood of patients with very severe chronic obstructive pulmonary disease

The carbonyl content in blood proteins in patients with chronic obstructive lung disease

The carbonyl content in blood proteins in patients with chronic obstructive lung disease

Assessment of metabolic and hormonal shifts in patients at risk of diabetes mellitus

Coronary patients and patients with essential hypertension were found to have the highest levels of glycated hemoglobin (gly-Hb), specifically, of its HbAlc fraction, as was shown in screening of 615 subjects. Measurements of blood levels of gly-Hb and gly-albumin, study of pyruvate metabolism, oral glucose tolerance test, and glucose-insulin test in 74 patients with coronary disease and essential hypertension revealed a high prevalence of latent disorders of carbohydrate metabolism in this patient population, with disorders of carbohydrate metabolism manifesting primarily by an increase of blood levels of glycated proteins and by disorders of pyruvate metabolism. Glycation of blood proteins in patients with coronary disease and essential hypertension increases blood viscosity (1.6 ± 0.02), spontaneous platelet aggregation (46.59 + 2.07% vs. 18.1 ± 1.5% in control), reduces fibrinolysis, and is conducive to a parallel increase of the concentration of blood serum lipids. These patients develop disorders of insulin secretion after glucose loading, thyroid function is reduced with the deficit of tri-iodothyronine production (1.46 + 0.07 nmol/liter vs. 1.92 ± 0.1 nmol/liter in health, p < 0.05); hydrocortisone hypersecretion is observed (746.25 ± 49.74 nmol/liter vs. 358.23 + 9.8 nmol/liter in health, p < 0.001); hormonal dysfunction favors the formation of glucose toxicity mechanism in normal glycemia when no clinical signs of impaired glucose tolerance are seen.