Protein In Pregnant Women Research Articles

Different Antigen-Specific CD4+ and CD8+ T-Cell Response against HCMV Proteins in Pregnant Women with Primary Infection and in Control Subjects with Remote Infection.

Background/Objectives: Human cytomegalovirus (HCMV) is the most frequent cause of congenital infections. The HCMV-specific T-cell response in primary infection may help define reliable correlates of immune protection in pregnancy. In this study, the antigen-specific T-cell response against different HCMV proteins (IE-1, pp65, gB, gHgLpUL128L) was investigated in pregnant women with primary infection and in control subjects with remote infection to identify possible components of a vaccine. Methods: Blood samples from 35 pregnant women with HCMV primary infection and 30 HCMV-seropositive healthy adult subjects with remote infection were tested. The antigen-specific T-cell response was measured using cytokine intracellular staining after stimulation with IE-1, pp65, gB and gHgLpUL128L peptides pool. Results: The pp65-specific CD4+ T-cell response was higher in pregnant women with HCMV primary infection at the late time point and in control subjects with remote infection, while the pregnant women at the early time point showed a higher gB-specific CD8+ T-cell response. Regarding the CD4+ and CD8+ T-cell phenotypes, we observed that HCMV-specific CD4+ and CD8+ T cells expressing CD45RA+ remained constant in pregnant women with primary infection at the early and late time points and in subjects with remote infection, while HCMV-specific CD4+ and CD8+ T cells expressing IL-7R+ or producing IL-2 were higher in control subjects with remote infection than in pregnant women with HCMV primary infection. Conclusions: The T-cell response was higher against gB in the early phase of infection and against pp65 in the late phase. Therefore, these proteins should be taken into consideration as candidates for a vaccine.

Altered Levels of Natural Autoantibodies against Heat Shock Proteins in Pregnant Women with Hashimoto's Thyroiditis.

The function of natural autoantibodies (nAAbs) in maintaining immunological tolerance has been comprehensively explained; however, their function in pregnant patients dealing with autoimmune diseases has not been thoroughly investigated. As Hashimoto's thyroiditis (HT) is the predominant organ-specific autoimmune condition of women of childbearing age, this study's objective was to evaluate IgM and IgG nAAbs targeting mitochondrial citrate synthase (CS) and heat shock proteins (Hsp60 and Hsp70) in women diagnosed with HT who were pregnant (HTP). Serum samples collected from HTP and healthy pregnant (HP) women in the first and third trimesters were tested using in-house-developed enzyme-linked immunosorbent assays (ELISAs). Our findings indicate the stability of nAAbs against CS and Hsps throughout the pregnancies of both healthy women and those with HT. However, during both trimesters, HTP patients displayed elevated levels of IgM isotype nAAbs against Hsp60 and Hsp70 compared to HP women, suggesting a regulatory role of IgM nAAbs during the pregnancies of patients with HT. Nonetheless, levels of IgG isotype nAAbs against Hsps were lower solely in the third trimester among HTP patients, resulting in a higher IgM/IgG ratio, which indicates their importance in alterations of the nAAb network during pregnancy in patients with HT.

Імунозапальні зміни важкого перебігу COVID-19 у вагітних

Pregnant women are the most sensitive contingent to the effects of the virus and this is due to adaptive physiological changes in the woman’s bronchopulmonary system and features of the immune system. “Physiological tolerance” is formed during pregnancy and this is accompanied by a decrease in the level of T-helpers and natural killers in pregnant women and leads to a decrease in the resistance of the woman’s body to the action of altering viral and bacterial agents. Purpose - to reveal the peculiarities and clarify the nature of clinical and instrumental changes in pregnant women with a severe course of COVID-19 under dynamic observation. Materials and methods. The study included 40 pregnant women with coronavirus disease with a gestational age of 22 to 41 weeks, who formed the main group. The control group consisted of 40 women whose pregnancies were not complicated by coronavirus disease. The course of the disease and the influence of the coronavirus infection on the course of pregnancy were evaluated according to the results of: general - clinical examination, laboratory examination (general blood test, biochemical blood test, coagulogram, C-reactive protein, D-dimer, procalcitonin and interleukin 6), instrumental examination (cardiotocographic research, pulse oximetry). Results. Peculiarities of changes in clinical and laboratory indicators in pregnant women were studied depending on the stage of development of the coronavirus disease. During the study, it was established: a significant increase in the level of indicators in accordance with the control group, namely an increase in leukocytes by 38.2% to 10.07±1.59×109/l (р<0.05), an increase in thrombocytes by 35% to 314.36±49.70 (р<0.05), an increase in monocytes by 22.5% to 7.08±1.12 and a decrease in lymphocytes to 13.46±2.13, which is 51.2% lower than the indicators of the control group. The level of C-reactive protein in pregnant women with COVID-19 is probably higher than in all groups of examined patients. The level of D-dimer exceeded the indicators of the control group in patients of all groups with the maximum values of indicators in patients at the time of admission to department of anaesthesiology and intense therapy (DAIT) and was 4867.42 ng FEU/ml, which was 6.3 times higher than the level of the control group (p<0.05). It should be noted that the amount of interleukin-6 in patients of all studied groups was statistically higher than that of the control group. Conclusions. Immune-inflammatory blood changes in pregnant women occur in the case of a severe course of COVID-19 and are accompanied by an increase in the level of interleukin-6 at all stages of the severe course of the disease. СRP is a marker of the severe course of the coronavirus disease in pregnant women. The identified changes are accompanied by coagulopathy and hypercoagulation with increased D-dimer content in patients with a severe course of the disease. The research was carried out in accordance with the principles of the Helsinki Declaration. The study protocol was approved by the Local Ethics Committee of all participating institutions. The informed consent of the patient was obtained for conducting the studies. No conflict of interests was declared by the authors.

The Role of Fibrinogen on Abortion Incidence in Pregnant Women with Toxoplasmosis

Toxoplasma gondii is the cause of toxoplasmosis. More than 60% of the world's population is infected with T. gondii. The prevalence of acute toxoplasmosis in pregnant women is 4.8 per 1000 women. The risk of fetal infection until 13 weeks of gestation is about 15% and after that the risk of infection increases and reaches 72% at 36 weeks of gestation. Fibrinogen is one of the most important acute phase proteins in pregnant women. Physiologically, there is an increase in blood procoagulant levels (include fibrinogen), but anticoagulant levels in the blood decrease during pregnancy and the performance of the fibrinolytic system weakens. In this condition, if the woman is infected with toxoplasmosis during pregnancy, the excessive elevation of coagulation factors may increase the risk of thromboembolism. T. gondii infection is known to increase IFN-γ secretion in sheep and rat experimental animals, then secrete fibrinogen-like protein 2 (fgl2) which plays a role in fibrin deposition and thrombosis. Fgl2 is a transmembrane prothrombinase that directly cleaves prothrombin to thrombin. The prothrombinase activity of Fgl2 was observed in abortion. Overall, the coagulation system gets stronger in pregnant women. However, the inflammatory process due to toxoplasmosis results in a prothrombic state situation associated with increased fibrinogen levels. Increased fibrinogen further increases inflammation. Excessive increases in fibrinogen increase the risk of thromboembolism that causes placental infarct and causes abortion.

Biochemical analysis of oxidative stress and purinergic system in pregnant women with gestational diabetes mellitus

Background: Pregnancy is characterized as a physiological period with greater sensitivity to insulin resistance and changes in oxidative stress. Purinergic signaling is directly related to diabetes, as this condition modifies the concentration of extracellular ATP and the level of degradation of ATP to adenosine. Objective: Analyze oxidative stress and the purinergic system in pregnant women with Gestational Diabetes Mellitus (GDM) and compare them with low-risk pregnant women (LR). Materials and methods: The research was of a quantitative approach of an experimental nature. The study was carried out at the Clínica da Mulher, which serves high-risk pregnant women, and at the Family Health Centers, which serves low-risk pregnant women, both located in Chapecó, Santa Catarina, Brazil. Results: From the analysis, it was observed that oxidative stress was increased in pregnant women in LR compared to pregnant women with GDM by increasing the concentration of TBARS and reducing the concentration of Carbonyl Protein in pregnant women with LR. Regarding the purinergic system, there was a significant decrease in the hydrolysis of the nucleotides ATP, ADP, and AMP in pregnant women with GDM, and a significant increase in the hydrolysis of ADA, also in pregnant women with GDM. Conclusion: Therefore, pregnant women with GDM have less oxidative stress compared to pregnant women in LR concerning TBARS and Carbonyl Protein markers, thus allowing a greater antioxidant defense mechanism. Furthermore, concerning the purinergic system, there is an increase in the activity of ADA, which is directly related to the immunosuppression process, a necessary condition for the protection of the fetus during the gestational period.

The Effect of Cassava-Dried Shrimp Crackers on Urine Protein in Maternity Hypertension in Maros District Health Center

Maternal Mortality Rate is a serious world health problem. According to the World Health Organization in 2017 around 295,000 women died and after pregnancy and childbirth in 2018 increased to 830,000. The main cause of maternal death is preeclampsia, therefore hypertension and urine protein as causes of preeclampsia need to be controlled. Research has shown that certain nutrients can improve blood pressure and urine protein. The research objective was to analyze the effect of cassava cracker consumption on urine protein in maternity hypertension. This type of research used quasi-experimental and the populations in this study were all Maternities at Cenrana and Tompobulu Health Center. The sample was 15 pregnant women at Puskesmas Cenrana as an experimental group and 15 pregnant women at Tompobulu health center as a control group. The results of the paired t test showed that the consumption of ebi cassava crackers at fourth’s week had a significant effect on changes in urine protein in pregnant women in intervention group with a value of p> α 0.05 = 0.008, while in the control group there was no effect with a value of p> α 0, 05 = 0.250. It was concluded that consumption of ebi cassava crackers during four weeks changed the urine protein from positive to negative. Hopely, every hypertensive pregnant woman consumes cassava crackers to prevent positive urine protein.

Levels of C-Reactive Proteins in Pregnant Women and its Relation with Preeclampsia in Singleton Pregnancy

Objective: To find the levels of C-reactive protein pregnant women and its relation with preeclampsia in singleton pregnancy. Study Design: Case control study. Place and Duration of Study: Department of Obstetrics & Gynaecology, Mohtarma Benazir Bhutto Shaheed Medical College, Mirpur Azad Kashmir from 1st July 2021 to 30th June 2022. Methodology: One hundred preeclampsia and 50 control women were enrolled. Protein in the urine presenting renal impairment, decrease platelet counts and renal disease were assessed. Cases that had a blood pressure which was chronically high in cases prior to gestation or were chronic renal disease patients were excluded from the study. A biochemical analysis of C-reactive proteins was conducted through ELISA. The results were than interpreted in terms of their association with preeclampsia. Demographic details and clinical parameters as body mass index (kg/m2), familial history, any related comorbidities, clinical signs and symptoms were documented. Results: The mean age of the preeclampsia women was 31.3±3.2 years while that of pregnant women who were normal controls as 25.8±3.8 years. Primigravida was higher in preeclampsia women while multigravida was higher in normal control pregnant women. Hypertension was prevalent in 73% of the preeclampsia cases than 10% of the normal control pregnant cases. Urinary protein was high as 2 gm in 51% cases of preeclampsia The C-reactive protein value was also significantly higher in preeclampsia cases than normal controlled pregnant women. Conclusion: There is a direct significant association between preeclampsia and high levels of C-reactive proteins. Keywords: C-reactive protein, Pre-eclampsia, Pregnancy

Progesterone Enhances the Invasion of Trophoblast Cells by Activating PI3K/AKT Signaling Pathway to Prevent Preeclampsia

We aimed to explore whether the effect of progesterone on preeclampsia via the PI3K/AKT signaling pathway. First, we studied the role of progesterone in preeclampsia patients and HTR-8/Svneo cells by adding progesterone. Then PI3K inhibitor LY294002 was added. The effects of progesterone on preeclampsia were also studied in animals by constructing a preeclampsia rat model. CCK-8 and Transwell assay were applied to measure cell viability and invasion ability. ELISA was performed to measure progesterone, MMP-2, MMP-9, pro-inflammatory factors TNF-α, IL-1β, and anti-inflammatory factors IL-4, IL-10, and IL-13 levels. HE staining was used to detect the pathological changes in uterine spiral artery. Western blot was performed to detect Cyclin D1, PCNA, MMP-2, MMP-9, inflammatory factors TNF-α, IL-1β, IL-4, IL-10, IL-13, and PI3K/AKT signaling pathway related proteins AKT, p-AKT, PI3K, and p-PI3K expressions. Progesterone could reduce blood pressure and urine protein in pregnant women with preeclampsia. TNF-α and IL-1β levels were decreased, but IL-4, IL-10, IL-13, cyclin D1, and PCNA levels were increased in pregnant women with preeclampsia after using progesterone. After the use of progesterone, the symptoms of the PE model group were improved. Among them, the lumen of the placental uterine spiral artery was enlarged, and the fibrinoid necrosis of the uterine wall and acute atherosclerotic lesions were relieved. In addition, progesterone promoted HTR-8/Svneo cells proliferation and invasion. However, high expression of MMP-2, MMP-9, p-AKT, and p-PI3K in Normal and preeclampsia groups caused by progesterone was weakened after adding LY294002, indicating that progesterone could activate PI3K/AKT signaling pathway to regulate HTR-8/Svneo cells. Progesterone decreased urine protein and blood pressure of preeclampsia rats in a concentration-dependent manner. Moreover, progesterone activated the PI3K/AKT signaling pathway and inhibited the inflammatory response in preeclampsia rats.

Gambaran Kadar Hemoglobin Dan Protein Urin Pada Ibu Hamil

Backgrounds of hemoglobin is a metalloprotein in red blood cells that functions as an oxygen booster from the lungs to the rest of the body. To maintain hemoglobin levels in the body, especially in pregnant women, it is necessary to have adequate intake of nutrients, namely iron. If the intake of iron in pregnant women is less, it will increase the risk of anemia. Anemia is a condition or condition that causes low levels of hemoglobin (Hb). Albuminuria or proteinuria is a condition in which urine or urine contains an abnormal amount of albumin. Throughout pregnancy, pregnant women are usually advised to take a urine test. One of the focuses of the urine test is to determine urine protein levels in pregnant women. In pregnant women, there is a phase where urine protein levels increase, namely, lack of fluids, exposure to cold and extreme weather, new strenuous activities, and emotional stress also often causes urine protein levels in pregnant women to increase, and impaired kidney function. or urinary tract infection. The method we used in this research is descriptive research method to describe the levels of hemoglobin (Hb) and urine protein in pregnant women at the Ubud 1 Public Health Center, Gianyar-Bali. With the sampling technique using the probability sampling method, with a simple random sampling technique. The results of 41 respondents, that pregnant women who have normal Hb levels (≥ 11 g/dL) at Puskesmas Ubud 1 were found to be more than pregnant women who have low Hb levels ( 11 g/dL). And from the total sample, as well as 2 pregnant women from the urine protein level examination data were positive. The conclusion after averaging the Hb and urine protein levels of pregnant women at the ubud 1 health center that it can be said that the health condition of pregnant women is quite good, but there are still some respondents who show results that are not in accordance with normal limits, therefore it is important to maintain health and pattern healthy living.Keywords: hemoglobin, urine protein, pregnant women

Ambient fine particulate matter exposures and oxidative protein damage in early pregnant women

The association between oxidative protein damage in early pregnant women and ambient fine particulate matter (PM2.5) is unknown. We estimated the effect of PM2.5 exposures within seven days before blood collection on serum 3-nitrotyrosine (3-NT) and advanced oxidation protein products (AOPP) in 100 women with normal early pregnancy (NEP) and 100 women with clinically recognized early pregnancy loss (CREPL). Temporally-adjusted land use regression model was applied for estimation of maternal daily PM2.5 exposure. Daily nitrogen dioxide (NO2) exposure of each participant was estimated using city-level concentrations of NO2. Single-day lag effect of PM2.5 was analyzed using multivariable linear regression model. Net cumulative effect and distributed lag effect of PM2.5 and NO2 within seven days were analyzed using distributed lag non-linear model. In all 200 subjects, the serum 3-NT were significantly increased with the single-day lag effects (4.72%–8.04% increased at lag 0–2), distributed lag effects (2.32%–3.49% increased at lag 0–2), and cumulative effect within seven days (16.91% increased). The single-day lag effects (7.41%–10.48% increased at lag 0–1), distributed lag effects (3.42%–5.52% increased at lag 0–2), and cumulative effect within seven days (24.51% increased) of PM2.5 significantly increased serum 3-NT in CREPL group but not in NEP group. The distributed lag effects (2.62%–4.54% increased at lag 0–2) and cumulative effect within seven days (20.25% increased) of PM2.5 significantly increased serum AOPP in early pregnant women before the coronavirus disease (COVID-19) pandemic but not after that, similarly to the effects of NO2 exposures. In conclusion, PM2.5 exposures were associated with oxidative stress to protein in pregnant women in the first trimester, especially in CREPL women. Analysis of NO2 exposures suggested that combustion PM2.5 was the crucial PM2.5 component. Wearing masks may be potentially preventive in PM2.5 exposure and its related oxidative protein damage.

Comparative Evaluation of C-reactive Protein In Pregnant Women with or Without Periodontitis: A Randomized Control Trial

Background: Serum C-reactive protein (CRP) is non-specific in nature and is seen to be associated with adverse pregnancy outcomes. Thus we aim to compare serum CRP levels in pregnant women with and without periodontitis. 
 Methods: A total of 222 pregnant women aged between 18-45 years were recruited in study and divided into two groups Group A:- Pregnant patient without periodontitis (n=100) and Group B:- Pregnant patient with periodontitis (n=122). Different clinical parameters such as plaque index (PI), gingival index (GI), probing depth (PD) and clinical attachment loss (CAL) were evaluated. A 2 ml of blood sample was taken from antecubital vein for quantative serum CRP analysis.
 Results: The mean CRP level in Group B was found to be higher (4.22 + 2.247 mg/L) than Group A (1.55 + 0.702 mg/L). In both the groups, statistically significant and positive correlation of PI with GI and CRP level was found (p < 0.05). Similarly, in Group B, PI showed a positive correlation with PD & CAL whereas, PD showed a similar correlation with CAL & CRP level was statistically significant. (p < 0.05).
 Conclusion: Periodontitis influenced the serum CRP levels in pregnant patients representing increased inflammatory burden.

Antibody levels to SARS-CoV-2 spike protein in mothers and children from delivery to six months later.

IntroductionPregnant women are vulnerable to severe acute respiratory syndrome coronavirus (SARS‐CoV‐2) infection. Neutralizing antibodies against the SARS‐CoV‐2 spike (S) protein protect from severe disease. This study analyzes the antibody titers to SARS‐CoV‐2 S protein in pregnant women and their newborns at delivery, and six months later.MethodsWe conducted a prospective study on pregnant women with confirmed SARS‐CoV‐2 infection and newborns. Antibody (IgG, IgM, and IgA) titers were determined using immunoassays in serum and milk samples. An angiotensin‐converting enzyme 2 (ACE2) receptor‐binding inhibition assay to the S protein was performed on the same serum and milk samples.ResultsAt birth, antibodies to SARS‐CoV‐2 spike protein were detected in 81.9% of mothers' sera, 78.9% of cord blood samples, and 63.2% of milk samples. Symptomatic women had higher antibody titers (IgG, IgM, and IgA) than the asymptomatic ones (P < 0.05). At six months postpartum, IgG levels decreased drastically in children's serum (P < 0.001) but remained high in mothers' serum. Antibody titers correlated positively with its capacity to inhibit the ACE2–spike protein interaction at baseline in maternal sera (R 2 = 0.203; P < 0.001), cord sera (R 2 = 0.378; P < 0.001), and milk (R 2 = 0.564; P < 0.001), and at six months in maternal sera (R 2 = 0.600; P < 0.001).ConclusionsHigh antibody levels against SARS‐CoV‐2 spike protein were found in most pregnant women. Due to the efficient transfer of IgG to cord blood and high IgA titers in breast milk, neonates may be passively immunized to SARS‐CoV‐2 infection. Our findings could guide newborn management and maternal vaccination policies.

Evaluation of Reproductive Hormone, Electrolytes and Serum Protein in Pregnant Women with Different Genotypes

Introduction: Genotype is an inward expression of gene or the combination of alleles, situated on corresponding chromosomes that determines a specific trait of an individual. Sickle Cell Disease is associated with higher clinical and obstetric complications compared to the general population.&#x0D; Aim: This study sought to investigate how different genotypes might affect the levels of hormones, electrolytes and serum proteins in pregnancy.&#x0D; Study Design: A case-Control Study.&#x0D; Methodology: Participants were 120 pregnant women recruited from hospitals in Oyo State and Ekiti State. They were grouped into 40each according to their Genotypes: AA,AS and SS. Blood samples were collected and analytes quantified by standard laboratory methods. One way analysis of variance (ANOVA) and Duncan multiple range test were used to compare variables across the three groups.&#x0D; Results: There was a significant reduction in the levels of Estrogen, and Prolactin in Sickle Cell group when compared to AA and AS group, while the levels of progesterone were not statistically different in the three groups. Also, there was a significant reduction in the levels of Na, K ,Total Protein and Albumin in Sickle Cell Group when compared to Group 1 and 2. However, the levels of Ca, Mg, Zn, Cl and Fe, C-reactive protein were not statistically different in the three groups.&#x0D; Conclusion: Low levels of reproductive hormones, total protein and electrolytes imbalance are associated with pregnancy in Sickle Cell Disease.

Association of CRP Levels in 3rd Trimester with Fetal Birth Weight in Normotensive and Preeclamptic Pregnant Females

Aim: To assess the level of C-reactive protein, which is an inflammatory marker, in pregnant women with pre-eclampsia and with normal blood pressure, and to assess its relationship with fetal weight at birth. Study design: An analytical and Cross-sectional study. Place and duration of study: In the Obstetrics and Gynecology department of Chandka Medical Hospital, Larkana and Murshid Hospital, Karachi for nine months duration from January to September 2021. Methodology: A total 70 cases of pre-eclampsia and 70 pregnant women with normal blood pressure participated, all in the 3rd trimester of gestation. All females were in the 20-40 years of age group and had a BMI ranging from 18 to 25. Highly sensitive levels of C-reactive protein (hsCRP) were evaluated using the Enzyme Link immunosorbent assay. Statistical analysis was performed with SPSS program 20.0. The values were taken as significant at the significance level of 0.05. Results: C-reactive protein levels were significantly advanced in the pre-eclampsia group with a mean value of 8.4 (0.4 to 24.2) compared to 5.1 (0.21 to 10.1) mg / L in women with normal blood pressure (p-value &lt;0.001). Spearman's correlation coefficient between birth weight and CRP was -0.405 in the pre-eclampsia group (p = 0.001) and 0.118 in the normal blood pressure group. In the group with pre-eclampsia, the birth weights of children were also significantly lower. High CRP levels in the preeclampsia group were negatively correlated with fetal birth weight. Conclusions: High levels of C-reactive protein in pregnant women with pre-eclampsia are part of the excessive systemic maternal inflammatory response and are associated with low fetal weight. Keywords: Pre-eclampsia. C-reactive protein. Pregnancy. Third trimester

The Role Of Pregnancy Age, Blood Pressure And Vitamin D Deficit On Urine Protein Levels In Pregnant Women: A Study In Rural Population

Preeclampsia is a potentially dangerous pregnancy complication characterized by high blood pressure and protein levels in the urine that are prone to occur in the third trimester. Urine protein levels have been known as a definite indicator of preeclampsia cases and are also determinants of the severity of preeclampsia experienced by pregnant women. The incidence of preeclampsia accounts for 3-8% of all pregnancies and is the cause of the most maternal deaths in Indonesia. The purpose of this study was to determine the risk factors that contribute to the severity of preeclampsia through urine protein levels in pregnant women. The Method used an Analytical observation design with a crossectional approach to analyze data obtained from 76 pregnant women in Muara Enim Regency, Indonesia, who met the inclusion criteria in this study. The results show a significant association of risky gestational age, blood pressure, and deficiency vitamin D with urine protein levels in pregnant women. At the same time, the age and Body Mass Index (BMI) of pregnant women proved not to be significantly related to the urine proteins of pregnant women. The Conclusion is Prevention and treatment can be done by knowing the predictors that cause the occurrence of urine protein in pregnant women. Education about the detection of risk factors becomes essential to be conveyed by health workers to mothers undergoing pregnancy or entering the second trimester of pregnancy.

Associations between the TNMD rs4828038 and ACE2 rs879922 polymorphisms and preeclampsia susceptibility: a case-control study

A case-control study was designed to investigate the association between the angiotensin converting enzyme 2 (ACE2) rs879922, glucose-6-phosphate dehydrogenase (G6PD) rs1050828, and tenomodulin (TNMD) rs4828038 single nucleotide polymorphisms (SNPs), and preeclampsia. A total of 356 Han Chinese pregnant women (170 controls and 186 cases) were recruited into the study. ACE2 rs879922, G6PD rs1050828, and TNMD rs4828038 were tested by the targeted next-generation sequencing technology and the data were analyzed using SPSS version 18. Genotyping of results revealed that patients with the CC/CT genotype in SNP rs4828038 or CC/CG genotype in SNP rs879922 had a significantly decreased susceptibility to late-onset preeclampsia (CC/CT versus TT: OR = 0.543, 95% CI = 0.378 to 0.779, p = .001; CC/CG versus GG: OR = 0.510, 95% CI = 0.038 to 0.860, p = .012). Our study found that the polymorphisms TNMD rs4828038 and ACE2 rs879922 might be associated with late-onset preeclampsia. IMPACT STATEMENT What is already known on this subject? Preeclampsia is associated with multiple SNPs, and ACE2 rs879922, G6PD rs1050828, and TNMD rs4828038 are related to essential hypertension and glucose and lipid metabolism disorders. Essential hypertension, diabetes, and dyslipidemia are risks for preeclampsia. The associations between those three SNPs and preeclampsia have not been reported. What do the results of this study add? The polymorphisms of TNMD rs4828038 and ACE2 rs879922 might be associated with the risk of late-onset preeclampsia. There was no relationship between SNP rs1050828 and preeclampsia. What are the implications of these findings for clinical practice and/or further research? TNMD rs4828038 and ACE2 rs879922 might be target sites for genetic diagnosis and therapy, and the levels of mRNA and protein in pregnant women with preeclampsia should be further tested.

The role of placental proteins in pregnant women during preparation for childbirth

<h3>ЦЕЛЬ ИССЛЕДОВАНИЯ</h3> Определение роли плацентарных белков — трофобластического бета-1-гликопротеина (ТБГ), гликоделина и плацентарного альфа-1-микроглобулина (ПАМГ) в процессе «созревания» шейки матки перед своевременными родами. <h3>МАТЕРИАЛ И МЕТОДЫ</h3> Обследованы 245 беременных в сроки беременности 38—39 нед, в том числе 65 со «зрелой» шейкой матки (1-я группа), 84 с «недостаточно зрелой» шейкой матки (2-я группа) и 96 пациенток с «незрелой» шейкой матки (3-я группа). Беременные 2-й и 3-й групп обследовались дважды в течение одной недели. Материалом для исследования служила периферическая венозная кровь, в сыворотке которой определяли концентрацию ТБГ методом двойной иммунодиффузии с использованием стандартной тест-системы, содержание ПАМГ и гликоделина — методом иммуноферментного анализа. <h3>РЕЗУЛЬТАТЫ</h3> У первородящих женщин с «незрелой» шейкой матки при доношенной беременности отмечается повышенный уровень ТБГ при неизмененных уровнях ПАМГ и гликоделина в периферической крови. В отсутствие динамики подготовки организма к родам повышенное содержание ТБГ в крови матери сохраняется, повышается концентрация гликоделина, уровень ПАМГ не меняется; при «созревании» шейки матки уровень ТБГ снижается. <h3>ЗАКЛЮЧЕНИЕ</h3> Можно предпожить, что ТБГ принимает участие в патогенезе ремоделирования шейки матки, роль гликоделина и ПАМГ не определена. Снижение уровня ТБГ у женщин с доношенной беременностью можно использовать в качестве критерия эффективности подготовки организма к родам.

Systematic proteomics analysis of lysine acetylation reveals critical features of placental proteins in pregnant women with preeclampsia.

Preeclampsia (PE) is a dangerous hypertensive disorder that occurs during pregnancy. The specific aetiology and pathogenesis of PE have yet to be clarified. To better reveal the specific pathogenesis of PE, we characterized the proteome and acetyl proteome (acetylome) profile of placental tissue from PE and normal‐term pregnancy by label‐free quantification proteomics technology and PRM analysis. In this research, 373 differentially expressed proteins (DEPs) were identified by proteome analysis. Functional enrichment analysis revealed significant enrichment of DEPs related to angiogenesis and the immune system. COL12A1, C4BPA and F13A1 may be potential biomarkers for PE diagnosis and new therapeutic targets. Additionally, 700 Kac sites were identified on 585 differentially acetylated proteins (DAPs) by acetylome analyses. These DAPs may participate in the occurrence and development of PE by affecting the complement and coagulation cascades pathway, which may have important implications for better understand the pathogenesis of PE. In conclusion, this study systematically analysed the reveals critical features of placental proteins in pregnant women with PE, providing a resource for exploring the contribution of lysine acetylation modification to PE.

Differences of heparin binding protein levels in preeclampsian and non preeclampsian women

This study aims to the incidence of preeclampsia and determine differences in heparin-binding protein levels in preeclamptic and non-preeclamptic mothers and determine the relationship between urinary tract infections in pregnancy. The research design was cross-sectional on 65 respondents with an accidental sampling technique. The research data were obtained from a questionnaire containing a list of questions given to respondents, measurement of middle urine samples to measure nitrite, leukocytes urine using dipstick method, and to determine heparin binding protein (HBP) levels using the ELISA method in the Hasanuddin University RSP Research Laboratory. The results of the study found there was a difference in the levels of heparin-binding protein in pregnant women without preeclampsia, namely 1.90±0.22 Ng/ml and HBP levels in pregnant women preeclampsia ie 2.39±0.3 Ng/ml. That there was no significant relationship between urinary tract infections (UTI) and the incidence of preeclampsia with p= 0.074. The conclusion in this study is that the levels of heparin-binding protein differ in preeclamptic and non-preeclamptic mothers, the increase in HBP levels in preeclampsia is due to inflammation, the cause is not through UTI. There is no association between urinary tract infections in pregnant women and the incidence of preeclampsia.

Use of data-independent acquisition mass spectrometry for comparative proteomics analyses of sera from pregnant women with intrahepatic cholestasis of pregnancy

We used data-independent acquisition (DIA) proteomics technology followed by ELISAs and automated biochemical analyses to identify and validate protein expression levels in Intrahepatic Cholestasis of Pregnancy (ICP) and healthy pregnant controls. We employed bioinformatics to identify metabolic processes associated with differentially expressed proteins.The expression levels of two proteins (S100-A9 and the L-lactate dehydrogenase A chain) were significantly higher in ICP patients than in controls; the areas under the receiver operating characteristic (ROC) curves (AUCs) were 0.774 and 0.828, respectively. The expression levels of two other proteins (apolipoprotein A-I and cholinesterase) were significantly lower in patients, with values of 0.900 and 0.842, respectively. Multiple logistic regression showed that a combination of the levels of the four proteins optimized the AUC (0.962), thus more reliably diagnosing ICP. The levels of all four proteins were positively associated with that of total bile acids. Bioinformatics analyses indicated that the four proteins principally affected neutrophil activation involved in the immune response, cell adhesion, lipoprotein metabolism, and the PPAR signaling pathway. SignificanceThis preliminary work improves our understanding of changes in serum levels of protein in pregnant women with ICP. The four proteins may serve as novel noninvasive biomarkers for ICP.