Protein Concentration In Blood Serum Research Articles

ELISA and Fourier-transform infrared spectroscopy

ELISA and FTIR assay techniques were used to identify HER2 gene expression in the blood serum of female dogs and to characterise the biochemical composition. ELISA tests assess the stage of primary tumour development and evolution, while FTIR allows for a complete characterisation of biomolecules associated with the tumoral process. Blood serum samples from 30 female dogs were analysed. Concentrations of the HER2/neu protein were detected using ELISA kits specific for canine and human detection. Infrared spectroscopy (IR) was conducted in absorbance mode at a frequency range of 400–4000 cm-1 and a resolution of 4 cm-1 over 50 scans. The ELISA cut-off for HER2 protein concentration in blood serum was determined using the receiver operating characteristic (ROC) curve and by estimating the area under the curve (AUC) at a 95% confidence interval (CI=95%). The ROC curves in the canine and human ELISA tests were 0.75 and 0.45, respectively. The representative IR spectra for HER2 gene expression corresponded to lipids (1161 cm-1, 1452 cm-1, 2851 cm-1). This study contributes to the knowledge of HER2 through the identification of biochemical features associated with the changes in the HER2/neu+ and HER2/neu- states.

Personalized approach to diagnostics and therapy of patients with psoriasis

Establishing causal relationship between allergy and psoriasis and determining appropriate sensitization profile may guide clinicians in terms of specific allergological diagnostics and personalized therapy. Our purpose was to study the occurrence of food allergy and certain sensitization profile to food allergens in patients with psoriasis, in order to establish new approaches to specific diagnosis and treatment of the disorder.The study involved patients with psoriasis vulgaris (n = 51) aged 18 to 66 years. The control group included practically healthy people matched by sex and age (n = 19). All the patients underwent allergological examination: collection of allergic history, determination of total immunoglobulin E (IgE) and eosinophilic cationic protein concentration in blood serum by means of indirect immunofluorescence analysis using Thermo Scientific Multiskan FC semi-automatic analyzer. The sensitization profiles to food and pollen allergens were also studied. Calculation and analysis of these data was carried out using Statistica 8.0 software package.Concentration of total immunoglobulin E in blood serum in the group of patients with psoriasis was 57.9 IU/ml (31.6-135.1) compared to control group, with 45.1 IU/ml (23.4-144.0). The levels of serum eosinophilic cationic protein was 8.6 ng/ml (4.6-20.3) in the patients with psoriasis, being 7.9 ng/ml (4.6-27.1) in controls. When studying the spectrum of sensitization to food allergens in patients with psoriasis, some specific features were revealed. E.g., sensitization to cow’s milk was detected in 33.3% (n = 17); to beef, in 39.2% of cases (n = 20), to whole chicken egg – 29.4% (n = 15), to chicken, in 39.2% (n = 20); to wheat flour, 37.2% (n = 19); to rye flour, in 31.4% (n = 16); to rice and buckwheat, 21.6% (n = 11), to yeast, in 23.5% of the patients (n = 12). Upon administration of individual elimination diet for 1 month, we observed a decrease or complete regression of itching, decreased severity of infiltration, hyperemia and peeling in the foci of psoriatic skin lesions in 68% of the cases. Normal concentration of total immunoglobulin E and eosinophilic cationic protein in blood serum of patients with psoriasis combined with positive results of skin-prick tests and elimination effect presumes a participation of non-atopic immune pathologies when triggering allergy in psoriatic disease.

EFFECT OF SUPPLEMENTAL ZINC AND COPPER ON PERFORMANCE OF GROWING RABBITS

Forty-two New Zealand White male rabbits with average body weight 546.74g were randomly assigned to 7 groups (6 animals in each) in a completely randomized design to study the effect of dietary zinc supplementation through inorganic (ZnO) or organic (Zinc EDTA) sources in combinations with copper (CuSo4) on growth performance. Rabbits of the first group (T1) were given basal diet without any supplementations (control group), while the other 6 groups were given basal diet containing zinc and copper as follows: group 2 (T2) received basal diet + 250 mg Cu/kg diet from CuSo4, group 3 (T3) received basal diet + 500 mg Cu/kg diet, group 4 (T4) received basal diet +20 mg Zn from Zinc EDTA + 250 mg Cu/kg diet, group 5 (T5) received basal diet + 20 mg Zn from Zinc EDTA + 500 mg Cu/kg diet, group 6 (T6) received basal diet +20 mg Zn from ZnO + 250 mg Cu/kg diet and group 7 (T7) received basal diet + 20 mg Zn from ZnO + 500 mg Cu/kg diet, respectively. Live body weight, daily body gain, daily feed intake andfeed conversion ratio were significantly increased by feeding rabbits on diets supplemented with 250 or 500 mg Cu/ kg diet. More enhancements were recorded in rabbits fed with 20 mg zinc as ZnO and 250 mg copper (T6). Total protein concentration in blood serum was significantly increased in rabbits fed 20 mg Zn from Zn EDTA + 500 mg Cu/kg diet (T5). Also the same treatment increased ceriatenine (CREAT) concentration in blood serum. While, Cu supplementations at 250 (T2) or 500 (T3) mg/kg feed increased urea concentration. All supplementation treatments significantly increased pre-slaughter weight comparing to un-supplemented control rabbits. The highest pre-slaughter weight was found in rabbits fed with diet containing 250 mg Cu/kg diet (T2) or with 20 mg Zn from ZnO + 250 mg Cu/kg diet (T6). Weights of carcass, liver and kidney and dressing (%) as well as weights of head and hind part did not significantly affected with the tested supplementations.Economically final margin was increased in all rabbits fed supplemented feed. The highest income from gain and the highest final margin were recorded in rabbit groups fed diet supplemented with 250 mg Cu/kg diet (T2) or 20 mg Zn from ZnO + 250 mg Cu/kg diet (T6).

Effect of Selank on Functional State of Rat Hepatocytes under Conditions of Restraint Stress.

We studied the effect of Selank administered intraperitoneally in doses of 100, 300, and 1000 μg/kg to male Wistar rats 15 min prior to restraint stress on the content of aminotransferases and total protein concentration in blood serum and intensity of free radical oxidation in the liver. Under conditions of acute restraint stress, Selank in doses of 100 and 300 μg/kg decreased catalase and superoxide dismutase activities and malondialdehyde concentration and increased total antioxidant activity in the liver homogenate. Administration of Selank in a dose of 1000 μg/kg reduced the content of aminotransferases in blood serum, decreased superoxide dismutase activity in the liver, and increased total antioxidant activity. Under conditions of chronic stress, Selank in all doses produced similar effects: reduced superoxide dismutase activity and malondialdehyde concentration in the liver tissue and AST activity in the serum. The other parameters remained unchanged.

Using Experimental Data Designs and Multivariate Modeling to Assess the Effect of Glycated Serum Protein Concentration on Glucose Prediction from Near-Infrared Spectra of Human Serum

Near-infrared (NIR) spectra of human blood serum consist of overlapping strong absorption bands of water and serum proteins, which affect the ability of multivariate calibration models to predict glucose. Furthermore, serum proteins such as albumin and globulins undergo a glycation reaction by forming covalent bonds with freely available glucose molecules in the serum. In diabetic individuals with poor glucose control, more and more serum protein molecules react with glucose, resulting in a high glycated protein concentration. The glucose molecules covalently bonded to serum proteins might contribute to the overall glucose signal acquired by NIR spectroscopy. This might affect the prediction ability of multivariate calibration models such as partial least squares regression (PLSR). In this study, we investigated the effect of total protein concentration and the glycated protein concentration in blood serum on the prediction ability of PLSR calibration models. Serum samples were subjected to ultra-filtration, and the PLSR model was built using NIR spectra of filtered serum solutions. Prediction performance was found to improve by 39-42% in absence of serum protein molecules. Various experimental data set designs were generated by carefully varying the glycated serum protein concentration in calibration and test sets of PLSR models. This investigation revealed that the impact of varying glycated protein concentration on the root mean square error of prediction was not drastic. To test the statistical significance of the prediction results, a multiple linear regression model was built. The glycated serum protein concentration was found to be statistically insignificant (p = 0.86) in predicting glucose concentration. Overall, it was concluded that the glycated serum proteins do not affect the glucose prediction accuracy of PLSR models using NIR spectra of human serum.

Efficiency of postoperated microwave therapy of the patients with inflammated tubo-ovarian tumors

The aim of the research is the study of efficiency of early postoperative rehabilitation of patients with purulent inflammatory formations of uterine appendages with microwave therapy. Postoperative treatment of 36 women-patients with the use of combined skin and intravaginal microwave exposure was done. The clinical characteristics of postoperative period and the results of the therapy in 18 month were studied. Dynamical changes of quantitative characteristics of cells at leukemia, of C-reactive protein, content in blood serum, in cervical canal and endometrium of tumor necrosis factor-alpha, interleukin-1 beta and interleukin-6 were estimated. If comparing with the patients who didn’t get physiotherapy exposure the use of microwave therapy at the patients with purulent tubo-ovarian formations was followed by earlier disappearance of inflammation, restoration of quantitative characteristics of leucocytes in blood, normalization of C-reactive protein concentration in blood serum and normal characteristics of erythrocyte sedimentation rate. Diminution of tumor necrosis factor-alpha in blood serum, interleukin-1 beta and interleukin-6, reduction of concentration in cervical canal and endometrium interleukin-6 were observed. In the postoperative period under the microwave therapy womenpatients had pain in pelvis, disturbance of menstrual cycle, 26,5% of women had the reproductive function to be restored.

Indices of collagen metabolism in pregnancy and postpartum period.

A progressive increase in total and free hydroxyproline and collagen-like protein concentration in blood serum was found in pregnancy, and high values of these indices were observed in the postpartum period as compared to nonpregnant women.

HEMOREFRACTOMETRY IN INFECTIOUS DISEASES OF CHILDREN

Refractometry is yet an almost blank chapter in pediatrics. In contrast to the now numerous papers on refractometry in adults, such researches in children are rare. On normal refractometry of blood serum in healthy children we know only the papers of Reiss1and Nast.2According to Reiss the refractometric index of blood serum in nurslings is lower than in adults, and this decrease agrees with about a 2 per cent. lower concentration of serum proteins. From the age of 6 to 10 months, says Reiss, the refractometric index rises and attains a definite degree, with a value varying from 1.3480 to 1.3514, as in adults. Refractometry is perhaps the best and surest means of serum protein estimation; Reiss's researches on the subject are too well known to need any detailed mention here. In order to estimate protein concentration in blood serum, we subtract from the number read 1.33320,