Protected Designation Of Origin Area Research Articles

Quality Evaluation of Bergamot Juice Produced in Different Areas of Calabria Region.

Citrus fruits are extensively cultivated worldwide, with Italy and Spain being major producers. In Southern Italy, particularly in Reggio Calabria, a typical citrus fruit is produced, namely, bergamot (Citrus bergamia Risso et Poiteau), known for its mysterious origins and exceptional quality essential oil protected by the EU's PDO (Protected Designation of Origin) designation. Despite historical challenges, bergamot has regained prominence for its nutraceutical potential, especially its flavonoid-rich juice, offering significant health benefits. However, little attention has been paid to understanding the qualitative and quantitative differences of bergamot juice in Calabrian production areas. For this reason, this work aims to investigate the quality characteristics of bergamot juice produced in different areas of Calabria sites. The results showed the best quality attributes of bergamot fruits harvested in the PDO area. In particular, higher levels of total soluble solids, stable acidity, and higher juice were found. In addition, higher contents of ascorbic and citric acids, which are nutritionally valuable and tasteful, were found. The phenolic profile, characterized by the key compounds of bergamot, highlighted the better nutraceutical potential of the fruit grown in the PDO area.

A Genotyping Method for Detecting Foreign Buffalo Material in Mozzarella di Bufala Campana Cheese Using Allele-Specific- and Single-Tube Heminested-Polymerase Chain Reaction.

Mozzarella di Bufala Campana (MdBC) cheese is a Protected Designation of Origin (PDO) product that is important for the economy and cultural heritage of the Campania region. Food fraud can undermine consumers' trust in this dairy product and harm the livelihood of local producers. The current methods for detecting adulteration in MdBC cheese due to the use of buffalo material from foreign countries could exhibit limitations associated with the required use of expensive equipment, time-consuming procedures, and specialized personnel. To address these limits here, we propose a rapid, reliable, and cost-effective genotyping method that can detect foreign buffalo milk in a counterpart from the PDO area and in MdBC cheese, ensuring the quality and authenticity of the latter dairy product. This method is based on dedicated allele-specific and single-tube heminested polymerase chain reaction procedures. By using allele-specific primers that are designed to detect the nucleotide g.472G>C mutation of the CSN1S1Bbt allele, we distinguished an amplicon of 330 bp in the amplification product of DNA when extracted from milk and cheese, which is specific to the material originating from foreign countries. By spiking foreign milk samples with known amounts of the counterpart from the PDO area, the sensitivity of this assay was determined to be 0.01% v/v foreign to PDO milk. Based on a rough estimate of its simplicity, reliability, and cost, this method could be a valuable tool for identifying adulterated buffalo PDO dairy products.

Where was my cup of honey made? PDO honey and sub-regional ethnocentric consumer segments

PurposeThe goal of this study is to understand if ethnocentrism exists at the sub-regional level among honey consumers living in the same production area as a protected designation of origin (PDO). Moreover, this analysis explores if ethnocentrism is influenced by individual economic conditions, among other socio-demographic characteristics.Design/methodology/approachA sample of 725 consumers was collected through the use of a questionnaire that was circulated in the province of Varese, one of the few honey PDO areas in Italy. The authors performed a principal component analysis and a two-step cluster analysis to identify different PDO honey consumer segments, focusing on their interest for PDO attributes.FindingsThe authors identified four consumer segments, depending on socio-demographic, consumption habits, frequencies, preferred attributes and preferences for the PDO product. One cluster exhibited strong preferences for the PDO honey, in the spirit of ethnocentrism, and was characterised by low-income levels; ethnocentric preferences were also observed in another cluster that had a different socio-economic profile.Research limitations/implicationsHoney is a niche product and not universally diffused among consumers: further analyses should investigate sub-national ethnocentrism for more universal food products. Yet, through the inspection of the different profiles found, it was possible to devise marketing strategies to boost PDO honey purchasing and to bring consumers closer to PDO products.Originality/valueThis analysis considers ethnocentrism as a segmentation criterion for PDO honey consumers that live in the very same PDO honey production area and enriches the existing literature on the relationship between ethnocentrism and individual economic status.

Rethinking vineyard ground management to counter soil tillage erosion

Tillage erosion is a relevant process of soil redistribution in sloping arable land, but little research has analysed the effect of shallow tillage on soil erosion in vineyards. The goal of this study was to quantify the soil tillage effect on soil translocation and erosion at the territorial level in a large vineyard area in Sicily. The soil loss and soil erosion tolerance limits were compared to identify the vineyards with a high risk of soil degradation. An alternative management scenario to traditional tillage was proposed to evaluate the effectiveness of the best management practices on soil conservation. The experimental trials were conducted in 14 vineyards with different slopes and soil characteristics. Soil translocation after shallow tillage was determined using coloured sand as the tracer. Regression results between the slope and mean translocation distance (T) were used to estimate tillage erosion (Qplot) in 2840 vineyard plots within a Protected Designation of Origin (PDO) area, considering the number of inter-rows and the length of each plot. After a single tillage operation in the downslope direction, the mean translocation distance ranged from 0.22 m to 0.45 m in the slope of 4% and 32%, respectively. At the plot level (Mg yr−1 per plot), the size and shape of the field must be considered, and the number of inter-rows was the predominant factor in tillage erosion estimation in vineyards. When comparing tillage erosion of each inter-row and soil erosion tolerance, the findings showed that 94% of the analysed plots had a high tillage erosion risk. Therefore, more attention should be paid to tillage operations in terms of type and intensity. The plots with high erosion risk decreased to 74% when best management practices with a cover crop in the entire PDO area were adopted. This study highlights the contribution of high tillage erosion to overall soil erosion losses, suggesting specific attention should be paid to more sustainable practices in soil management.

Success of organic and biodynamic system experiment to produce high quality wines

Reducing input use and in particular pesticide use is very important in OIV 2018 new resolutions. Also Europe and France promote research and co-design with winegrowers in more sustainable viticulture according to a combination of climate change. It is also very important to take into account soil diversity and Protected Designation Areas constraints to make sure that new wines will ensure the sustainability of the wineries. This study explores design, experiment and assess of new realistic viticulture production systems located in Protected Designation of Origin (PDO) areas and with already organic or biodynamic certification for six consecutive years, in winery production conditions. It also discusses the chosen assessment indicators and the adoption of such new production systems by winegrowers. Eleven cases were studied in a system experiment network in Alsace (France) and in six consecutive production years. Contrasted production years, PDO areas combined to organic or biodynamic certification allow testing the resilience of experimented systems over time. Combination of new practices are tested to drastically reduce input and in particular pesticide. Assessed performances of the systems deal with 14 main indicators: social acceptability (1) and economic viability (1), agronomy (yield (1), harvested berries quality (2), fungi damage (3), soil quality (1), and wine sensorial analysis (1) and environment (Frequency Treatment Index (1), Cupper rate (1), INDIGO® method (2)). Several innovations were selected by co-design with winegrowers: adding essential oil and Propolis to copper spraying; total grass cover of the vine site; new decision rules for rate and time pesticide spraying; decision aid tool; resistant grape varieties planted. The assessment results support that the performance of the 14 indicators is very good for most of the eleven systems during the six assessed years. Combinations of tested innovations are neither dangerous nor difficult to enforce in the field by the vineworkers. Innovations do not increase the cost of grape production. The yield ratios were satisfactory the 6-yeared of observation because calculated yield match with targeted yield. Harvested berries quality support that the 7 vine sites succeed in targeted total acidity and sugar rate according to the different PDO conditions every years. Wines were Alsace or Grand Cru PDO labelled and successful marketed. At least, the Treatment Frequency Index is reduced by an average of 40% for all vine sites and assessed years. TFI can be reduced at a maximum of 89% and TFI median is 38%. Cupper rate can be reduced at a maximum of 97% and Cupper rate median is 54%. I-pest from INDIGO® method indicate a minimal risk taking for groundwater, surface water, air and beneficial organisms contamination. And finally, flora richness indicator is stable or steadily increases during the 6 study years. In this study, we demonstrated that drastically monitoring reduction of pesticide and other inputs is possible. Chosen indicators allow a exhaustive assessment, but could also been aggregated all together to give a synthetic information to winegrowers and make them easier to adopt the innovative systems. Introduction of innovation combinations in highly sustainable organic and biodymanic systems are validated to produce high quality wines. Now, it is possible to serenely promote and disseminate these highly sustainable innovative systems, taking into account of course vineyard diversity.

Variability in chemical and microbiological profiles of long-ripened Caciocavallo cheeses.

Five protected designation of origin (PDO) Caciocavallo Silano and 6 non-PDO Caciocavallo cheeses, ripened for 6mo and collected in the 5 Italian regions of the PDO area (Apulia, Basilicata, Calabria, Campania, and Molise, Italy), were studied to assess their physico-chemical (pH, acidity, moisture, fat, ash, protein, and free amino acid composition) and microbiological profiles. Analyses evidenced a certain fluctuation of previous parameters among samples regardless of the kind of cheese evaluated (PDO and non-PDO). The PCR-denaturing gradient gel electrophoresis analysis performed on the DNA directly extracted from cheeses gave different results, but a low number of bands was always observed. Only one band, corresponding to the species Streptococcus thermophilus, was detectable in 1 PDO and in 2 non-PDO cheese samples, whose free amino acid content was the lowest. Analyses were repeated on experimental Caciocavallo cheeses. Specifically, 2 productions were made, one mimicking the industrial technology (pasteurized milk and selected starter culture) and one the artisanal technology (raw milk and natural whey starter). Results obtained on experimental cheeses at 6mo of ripening showed that industrial samples had lower amounts of total free amino acids then the artisanal ones (1,188.2 vs. 7,523.67mg/100 g of dry matter). Moreover, the PCR-denaturing gradient gel electrophoresis analysis evidenced the sole presence of S. thermophilus in the case of the industrial technology. These data sustain the hypothesis that, out of 11 cheeses analyzed previously, 1 PDO Caciocavallo Silano and 2 non-PDO Caciocavallo cheeses were obtained with the industrial technology. These results could be of help in the discrimination of PDO products, taking into account that the PDO production regulation does not allow the milk pasteurization, nor the use of selected starters.

Phenols, Volatiles and Sensory Properties of Primitivo Wines from the “Gioia Del Colle” PDO Area

The aim of this study was to characterise the phenol, anthocyanin, volatile and sensory profiles of Primitivo wines. The wines were produced in three wineries located in the Gioia del Colle PDO (Protected Designation of Origin) area (Southern Italy – Apulia Region). The grapes came from three vineyards of different ages and were grown according to different training systems. The winemaking techniques applied also differed in some technological variables. The results obtained showed that all wines had a high alcohol content (15 to 16% v/v) and were rich in total phenols and proanthocyanidins. The anthocyanin profile was characterised by the prevalence of non-acylated forms, of which malvidin-3-O-monoglucoside accounted for 62 to 67% of the total anthocyanin, followed by the coumarate, acetate and caffeate forms. The volatile fraction was constituted mainly alcohols and esters, the latter having concentrations above the odour threshold. From a sensory point of view, all wines were judged positively, and presented high olfactory and gustatory persistence. The main odour attributes found were soft fruits, cherry and cloves.

A Multivariate Methodological Approach to Relate Wine to Characteristics of Grape Composition: The Case of Typicality

Typicality is defined as the characteristics of a Protected Designation of Origin (PDO) wine related to a terroir. In the context of a PDO, a typicality assessment can be used to assess the global quality of a PDO wine. This study used a two-phase method to identify the distinctive characteristics of a PDO wine and then apply those findings to a case study of Chianti Montespertoli DOCG wine, where DOCG is the Italian acronym for PDO. A panel of wine professionals evaluated the typicality of a series of selected PDO wine samples, and a partial least squares (PLS) regression model was used to associate wine typicality and chemical composition. The model was used to predict wine typicality from the chemical composition of experimental wines (2009, 2010, and 2011 vintages) produced from grapes growing in the PDO area. Finally, regression coefficients of the PLS model were analyzed to select which grape chemical parameters were significant in describing the typicality of the wine. These highlight the most important factors to manage during winemaking to ensure the typicality of this wine.

Standard output versus standard gross margin, a new paradigm in the EU farm economic typology: what are the implications for wine-grape growers?

A new farm economic typology has recently been introduced in the EU. This study compares the economic performance of wine-grape growers in four important quality wine areas of Sicily taking into account the different EU economic typology standards of standard gross margin (the old method) and standard output (the new method). The objective of this study is to identify the differences between the two methods and to ascertain the potential implications for small wine-grape growers’ profitability and their access to public support. We seek to determine whether restrictions can be identified that limit the potential benefits from rural development policies.For this purpose, we applied the economic criteria of the EU to a representative sample of Sicilian wine-grape growers in order to compare the economic profitability of wine-grape farms in protected designation of origin areas. This comparative approach has allowed us to categorize the economic size of the sample in accordance with recent EU modifications and discuss the possible impacts that the adoption of the new economic criteria would have on access to the Rural Development Programme measures for wine-grape growers.

Use of Production Practices and Sensory Attributes to Characterize Loire Valley Red Wines

Quality wines are typically the product of soil, climate, and wine production practices. Sensory attributes are increasingly used to distinguish among Protected Designation of Origin (PDO) wines. Since 2010, the International Organization of Vine and Wine (OIV) is now taking all such parameters into account in the official ‘terroir’ definition. However, links between production practices and sensory attributes in geographically circumscribed PDO areas have not yet been investigated. This paper assesses production practices and sensory attributes of distinct PDO wines, within the same area. The study also identifies relationships between production practices and sensory attributes of these PDO wines. A survey was conducted to characterize production practices of thirty-three commercially produced PDO wines from the Loire Valley, France. Vineyard training systems, viticulture, harvest timing, winemaking and aging practices were studied. Descriptive sensory analysis was conducted to characterize the significant sensory attributes of these same wines. PDO wines were separated from one another by classical statistical analyses. About 25 percent of the analyzed production practices and less than fifty percent of the analyzed sensory attributes could differentiate PDO wines produced in the same area: respectively, percentage of Cabernet franc, time of leaf removal, harvest date, yield, duration of fermentation, duration of aging and use of oak, color intensity, viscosity, cloudiness, blackcurrant, prune, spicy, moldy, animal, bitterness and astringency. The study also identified links between some production practices and sensory attributes. The results of the study demonstrate that, under similar climatic conditions and soil characteristics, production practices and sensory attributes can distinguish PDO wines in well-defined geographic areas.

Characterisation of PDO olive oil Chianti Classico by non-selective (UV–visible, NIR and MIR spectroscopy) and selective (fatty acid composition) analytical techniques

An authentication study of the Italian PDO (protected designation of origin) extra virgin olive oil Chianti Classico was performed; UV–visible (UV–vis), Near-Infrared (NIR) and Mid-Infrared (MIR) spectroscopies were applied to a set of samples representative of the whole Chianti Classico production area. The non-selective signals (fingerprints) provided by the three spectroscopic techniques were utilised both individually and jointly, after fusion of the respective profile vectors, in order to build a model for the Chianti Classico PDO olive oil. Moreover, these results were compared with those obtained by the gas chromatographic determination of the fatty acids composition. In order to characterise the olive oils produced in the Chianti Classico PDO area, UNEQ (unequal class models) and SIMCA (soft independent modelling of class analogy) were employed both on the MIR, NIR and UV–vis spectra, individually and jointly, and on the fatty acid composition. Finally, PLS (partial least square) regression was applied on the UV–vis, NIR and MIR spectra, in order to predict the content of oleic and linoleic acids in the extra virgin olive oils. UNEQ, SIMCA and PLS were performed after selection of the relevant predictors, in order to increase the efficiency of both classification and regression models. The non-selective information obtained from UV–vis, NIR and MIR spectroscopy allowed to build reliable models for checking the authenticity of the Italian PDO extra virgin olive oil Chianti Classico.

Lipid and fatty acid profiles of Castanea sativa Mill. Chestnuts of 17 native Portuguese cultivars

In recent years, chestnuts have increasingly become important in human nutrition because of their nutrient contents and potential beneficial health effects. In Portugal, Trás-os-Montes e Alto Douro is the most important region for chestnut production, generating 85% of the total national output. In 1994, three chestnut protected designation of origin (PDO) areas were created: Terra Fria, Soutos da Lapa, and Padrela within the jurisdiction area of the Regional Agricultural Services of Trás-os-Montes Region. Seventeen cultivars were selected from these PDO to evaluate crude fat content and fatty acid (FA) composition over a period of 2 years. Total fat extraction was performed according to AOAC methodology, using a Soxhlet apparatus for 16 h. Petroleum ether was used for extraction and FAs were identified and quantified by gas chromatography. The cultivars studied confirm that chestnuts have low crude fat content, low saturated fatty acids (SFA) (17%) and high unsaturated fatty acids (USFA) (83%). The major FAs are linoleic, oleic and palmitic; these accounted for more than 85% of the total FA content. From the results two potentially health-beneficial cultivars were identified: cv. Lada, with high linoleic acid content, and cv. Aveleira, with higher USFA content. With respect to FA profiles of chestnuts from the three PDO regions, Padrela chestnuts consistently contained the highest level of linoleic acid. The variation detected among cultivars and PDO areas may reflect differences in genotype and are influenced by environmental and growing conditions. The Longal chestnuts, the only traditional cv. growing on two different PDO areas, had better nutritional qualities in PDO Terra Fria, which confirm that the ecological conditions where chestnut orchards are established influences nut quality.