ProTaper Universal Rotary Files Research Articles

The Influence of Nickel-Titanium (Ni-Ti) Rotary Instrument Systems on Debris and Smear Layer Formation in Endodontic Procedures: An In Vitro Scanning Electron Microscopy Study.

Background Successful endodontic treatment relies on the effective removal of debris and the prevention of smear layer formation within the root canals. The choice of nickel-titanium (Ni-Ti) rotary instrument systems can significantly impact these outcomes. Aim This study aims to evaluate and compare the debris and smear layer formation in root canals of extracted mandibular second premolar teeth following instrumentation with the ProTaper Universal (Dentsply Sirona,Charlotte, NC) (Group II), Twisted File (Kerr Endodontics, Gilbert, AZ) (Group III), and XP Endo (FKG Dentaire,La Chaux-de-Fonds, Switzerland) (Group IV) Ni-Ti rotary instrument systems. Methods In this in vitro study, 60 extracted mandibular second premolar teeth were randomly divided into four groups, each containing 15 teeth. Group I served as the control with no instrumentation. Groups II, III, and IV were instrumented with the ProTaper Universal rotary file, the Twisted File, and the XP Endo file systems, respectively. Debris and smear layer formation were evaluated through scanning electron microscopy (SEM), and photomicrographs were scored using a standardized index. Results Group II (ProTaper) exhibited the highest mean debris and smear layer scores, with values of 3.50 and 2.70, respectively. Group IV (XP Endo) demonstrated the least debris and smear layer formation, with mean scores of 2.65 and 2.08, respectively. Statistical analysis confirmed significant differences among the groups for both debris and smear layer formation. Conclusion The results highlight the practical importance of selecting appropriate Ni-Ti rotary instrument systems to minimize debris and smear layer formation during endodontic procedures. The XP Endo file system showed promise as a favorable choice in this regard, but further clinical research is needed to validate these findings.

Performance of two different electronic apex locators during the removal of calcium silicate-based sealers

Aim: The aim of this in vitro study is to evaluate the accuracy of Root ZX and Propex Pixi during the retreatment of root canals obturated with different calcium silicate-based root canal sealers. Methodology: Root canals of ninety human premolar teeth were shaped with ProTaper universal rotary files up to size F2 under 5.25% NaOCl irrigation for the present study. Root canal filling was carried out by lateral condensation technique using with gutta-percha and sealers; MTA Fillapex, CeraSeal or AH Plus. After seven days, the obturating materials were removed using D-Race files. While actual working length was recorded by direct observation, electronic working lengths were determined by Propex Pixi or Root ZX for tolerance limits of 0.5 mm and 1.0 mm. Data were analyzed using Shapiro Wilk, Mann-Whitney U, and Kruskal-Wallis tests (p < 0.05). Results: The performance of Propex Pixi and Root ZX was found to be statistically similar and did not differ on the root canals filled with different root canal sealers (p < 0.05). Propex Pixi and Root ZX demonstrated more accurate measurements in the range of ±1 mm compared to ±0.5 mm in all groups, regardless of the type of root canal sealer used when filling the root canals before retreatment (p < 0.05). Conclusion: Root ZX and Propex Pixi can be used confidently in the retreatment of the root canals in which CeraSeal, MTA Fillapex, and AH Plus are used as sealers. How to cite this article: Göçer PA, Durmazpınar PM, Baştürk FB. Performance of two different electronic apex locators during the removal of calcium silicate-based sealers. Int Dent Res 2023;13(S1):20-25. https://doi.org/10.5577/idr.2023.vol13.s1.3 Linguistic Revision: The English in this manuscript has been checked by at least two professional editors, both native speakers of English.

Use of final irrigants MTAD, Salvedora Perscia, Malachite green, and Ti-sapphire laser on push-out bond strength of Zirconia post

AIM: Evaluation of the push-out bond strength (PBS) of zirconia post-bonded to radicular dentin after using different final irrigants (MTAD, Malachite green (MG), Ti-sapphire laser and Salvadora persica (S.persica). MATERIAL AND METHOD: Forty human permanent single-rooted were decoronated above the cement-enamel junction. An experienced endodontist performed all the root canal instrumentation using ProTaper universal rotary files. Canals were irrigated using 5.25% NaOCl solution followed by EDTA as a final sterilant. Obturation with gutta-percha using AH Plus sealer was performed. Post-space preparation was completed using Gates Glidden and specimens were randomly assigned to four groups based on the final disinfectant received (n=10). Group 1: 5.25% NaOCl + MTAD, group 2: 5.25% NaOCl + MG, group 3: 5.25% NaOCl +Ti-sapphire laser, and group 4: 5.25% NaOCl + S. persica. The chemically polymerized resin was used to lute zirconia posts. PBS and failure mode analysis were performed using a universal testing machine and stereomicroscope at 40X magnification. Data were compared between the two groups with 95% CI using one-way analysis of variance (ANOVA) with the Tukey post hoc test. p=0.05. RESULTS: Group4(5.25% NaOCl+ S.persica) specimens demonstrated the maximum (8.94±0.14 MPa) bond strength. Conversely, the apical third of Group 2 (5.25% NaOCl+ MG) (2.87±0.15 MPa) samples revealed the minimum bond strength scores. Intergroup comparison exposed that Group 1 (1.3% NaOCl+ MTAD), Group 3 (5.25% NaOCl+Ti-sapphire laser), and Group 4 (5.25% NaOCl+ S. persica) at all three-thirds unveiled no significant difference in PBS (p<0.05). CONCLUSION: Ti-sapphire laser and Salvedora Persica possess the potential to be used as a final root canal irrigant to improve the push-out bond strength of zirconia post-to-root dentin.

A comparative evaluation of dentinal defects after root canal preparation with different rotary and reciprocal systems

Aim: This study aimed to compare dentinal defects after root canal preparation with various reciprocating and continuous rotary files.Methods: 90 extracted human mandibular incisor teeth were used. 15 teeth left unprepared and the remaning teeth were randomly divided into 5 experimental groups (n=15). Root canals were prepared with WaveOne (Dentsply Maillefer, Ballaigues, Switzerland), WaveOne Gold (Dentsply Maillefer, Ballaigues, Switzerland), Hyflex EDM OneFile (Coltene/Whaledent, Altstätten, Switzerland), ProTaper Next (Dentsply Maillefer, Ballaigues, Switzerland), and ProTaper Universal (Dentsply Maillefer, Ballaigues, Switzerland) rotary files. Then roots were sectioned at 3, 6 and 9 mm from the apex and evaluated with a stereomicroscope. Statistical analysis was performed with chi-square and Fischer’s Exact test. The significance level was set at 5%.Results: No defects were observed in the unprepared control group. Dentin defect were observed in all the experimental groups, especially in the apical region (3 mm). WaveOne and Hyflex EDM showed more dentinal defects than the control group (p0.05). WaveOne caused significantly more dentinal defects than the ProTaper Universal, ProTaper Next, and WaveOne Gold groups (p

Evaluation of pH and Calcium Ion Diffusion from Intracanal MTA and Bioaggregate to Simulated External Resorption Cavities Through Dentinal Tubules

Purpose: This in vitro study compared the effects of mineral trioxide aggregate (MTA), BioAggregate and calcium hydroxide on calcium and hydroxyl ion diffusion through dentinal tubules to the root surfaces without cementum in various root levels. Materials &amp;amp; Methods: The study consisted of 120 mature single-rooted mandibular premolar teeth. The teeth were decoronated and instrumented using Protaper Universal rotary files. To simulate external root resorption, artificial defects were created in cervical-middle-apical thirds of root surfaces. The teeth were divided into four main groups: Control (1), calcium hydroxide (2), MTA (3) and BioAggregate (4). In control group, root canals of specimens left empty. Other root canals of specimens filled with calcium hydroxide, MTA or BioAggregate. Each specimen was immersed in a vial containing 10 mL distilled water. Calcium concentrations and pH of the immersion media was measured at 1, 3, 7, 14, 21 and 28 days. Statistical analysis was accomplished by Kruskal–Wallis H and Mann–Whitney-U tests. Results: This study indicated that intracanal placement of both calcium hydroxide, MTA and BioAggregate resulted in the diffusion of calcium and hydroxyl ions across dentine. The results showed greater calcium and hydroxyl ions released by calcium hydroxide groups than MTA and BioAggregate groups (p&amp;lt;0.05). MTA and BioAggregate groups showed similar properties and there were no differences between these groups in whole study period (p&amp;gt;0.05). Conclusion: MTA and BioAggregate may be preferable for external root resorption cases because of their stimulation of hard tissue formation and ion releasing ability.

APICAL SEALING ABILITY OF GUTTA PERCHA AND C-POINT WITH DIFFERENT SEALERS- AN EX-VIVO

BACKGROUND- An apical seal is one which forms a barrier between the root canal and periapex. A three-dimesional seal is created by use of Gutta Percha and an endodontic sealer, which will fill the discrepancies between the core material and dentinal walls. Since years Gutta Percha has played a versatile role in root canal obturation; but newer materials have been introduced to overcome the hydrophobic nature of it. One such material is C-Point, a premade obturation point, which absorbs residual moisture (hydrophilic nature) from the canals and expands laterally to conform to canal complexities.MATERIALS AND METHOD- Fifteen freshly extracted mandibular incisors were prepared with Protaper Universal rotary files, and obturated by dividing into three groups of 5 teeth each. Group I- Gutta-percha &amp; AH-Plus, Group II, Gutta-percha &amp; Nanoseal S, and Group III C-point &amp; BioRoot RCS. After coating the teeth with nail polish except the apical 2 mm, they were suspended in methylene blue dye for 12 hour. They were then sectioned longitudinally and observed under stereomicroscope for the linear dye penetration. All the groups were compared by using ONE WAY ANOVA.RESULTS- One way ANOVA showed Group I with minimum microleakage (Mean ± SD) of 3.29±1.32 μm, followed by Group II 4.25±1.83 μm and Group III with maximum micro-leakage 5.53±1.72 μm.CONCLUSION- Gutta percha when used with AH-Plus showed better sealing ability as compared to Gutta Percha and Nanoseal S. The newer material, C-Point with BioRoot RCS showed the least sealing ability.

Apical Sealing Ability of Experimental Apatite Calcium Phosphate based Coated Gutta-percha Points

Aims: This study aimed to assess the apical sealing ability of experimental apatite-coated gutta-percha points compared to various coated gutta-percha root filling materials.&#x0D; Study Design: Experimental in-vitro study.&#x0D; Place and Duration of Study: Department of Restorative Dentistry, University of Malaya, June 2014 and November 2014.&#x0D; Methodology: Ninety-six extracted permanent maxillary central incisors were standardized and prepared by ProTaper universal rotary files. The specimens were allocated into six groups (n=15) and filled using matching single cone technique as follows; EndoREZ points with EndoREZ sealer, Bioceramic coated points (BCGP), Active GP (ActiV GP), Conventional gutta-percha (GP), apatite coated GP (HAGP) all in combination with Endosequence BC sealer. The sixth group was filled using cold lateral compaction of Conventional GP and Endosequence BC sealer. The specimens were subjected to a dye leakage test. The remaining six teeth were divided equally to be used as a negative and positive control. One-way analysis of variance (ANOVA) followed by the post hoc Dunnett T3 test was performed for data analysis, and the significance value was set at P =.05.&#x0D; Results: ActiV GP had the least leakage followed by BCGP, HAGP, cold lateral compaction, conventional GP, and EndoRez.&#x0D; Conclusion: HAGP as root canal filling showed favourable sealing ability comparable to ActiV GP, BCGP, and conventional GP and it was superior to EndoREZ.

A comparative evaluation of retrievability of three different obturating systems using protaper universal rotary retreatment files: An in vitro cone-beam computed tomography analysis

Aim: The aim of this in-vitro study was to comparatively evaluate the ease of retreatment in canals, obturated through GuttaFlow 2, GuttaCore, and conventional Lateral compaction technique using ProTaper Universal Retreatment (PTUR) Files.Materials and Methods: Sixty single-rooted mandibular premolars were selected and the canals were instrumented with ProTaper Universal rotary files up to size #F3. Samples were randomly divided into three experimental groups of 20 teeth each and obturated using three different obturating systems (GROUP I-Lateral compaction technique, GROUP II-GuttaFlow 2, GROUP III-GuttaCore). All the groups underwent cone-beam computed tomography (CBCT) analysis following which retrieval of the previous root canal filling was done using the PTUR files. Post retreatment CBCT images were used to assess the amount of remaining obturating material at varying depths (3 mm, 6 mm, and 12 mm) for all three groups. Statistical analysis was performed using repeated measures analysis of variance and ANOVA.Results: Irrespective of the obturating system used, filling material could not be removed completely from the root canal walls. Significantly more amount of obturating material was observed in the apical third than the middle third and coronal third of the root canal space (P < 0.05). When comparing the groups, the maximum percentage of remaining obturating material was seen in Group III (GuttaCore) followed by Group I (lateral compaction), with the least being observed in Group II (GuttaFlow2).Conclusion: The GuttaCore technique utilizing carrier-based gutta-percha had the maximum amount of remaining obturating material after retreatment when compared to the GuttaFlow2 and Lateral Compaction techniques.

Efficacy of Rotary Retreatment Techniques Assisted with Passive Ultrasonic Activation of Resin Solvent in Removal of Gutta-percha with Epoxy Resin and MTA Based Root Canal Sealers: An In-vitro Study

Introduction: Endodontic retreatment is indicated in cases of failed primary endodontic treatment. The main goal of retreatment is to establish conditions that will enable the healing of the periapical tissue. Aim: To evaluate the efficacy of rotary retreatment with the ProTaper Universal Retreatment system supplemented with passive ultrasonic activation of solvent in retreatment of singlerooted mandibular premolar teeth obturated with gutta-percha and two different root canal sealers (AH Plus and MTA Fillapex). Materials and Methods: This in-vitro study was conducted in the Department of Conservative Dentistry and Endodontics at Mahatma Gandhi Mission’s Dental College and Hospital, Navi Mumbai, Maharashtra, India, from January 2019 to April 2019. Total 66 freshly extracted human single rooted mandibular premolars were used in this study. Root canal preparation was done using ProTaper Next rotary files. Teeth were decoronated to get a uniform length of 18 mm and were divided into three retreatment groups based on the technique used for retreatment. Group I included rotary retreatment, Group II involved rotary retreatment with solvent and paper point wicking, and Group III involved rotary retreatment with passive ultrasonic activation of solvent and paper point wicking. Based on the sealer used for obturation, teeth in each group were further divided into two subgroups, subgroup A with AH Plus sealer and subgroup B with MTA Fillapex sealer. Teeth were stored in an incubator for 30 days to allow sealer to set after which sectioning of the specimens was done buccolingually for examination under the stereomicroscope and photographed. Statistical analysis was done by using parametric Analysis of Variance (ANOVA) followed by Post-hoc tests and Unpaired t-test. Results: No statistically significant difference in percentage area of residual root canal filling material noted between the group I and II and when obturated with gutta-percha and AH Plus sealer (subgroup A) as p-value=0.215. Whereas, it revealed a statistically significant difference between the group I, II, and III when obturated with gutta-percha and MTA Fillapex sealer (subgroup B) as p-value&lt;0.001. Conclusion: Passive ultrasonic activation of solvent after guttapercha removal using ProTaper Universal rotary files could improve the quality of endodontic retreatment.

Effect of Different Instrumentation and Irrigation Methods on Apical Microbial Extrusion: An Ex Vivo Study

Background: Periapical extrusion of debris, irrigating solution and microorganism are the major contributing factors for flare-ups during root canal therapy. The aim of this ex vivo study was to evaluate the effect of different types of instrumentation in combination with different irrigation methods on apical bacterial expulsion. Material and Methods: Three hundred and ten extracted human permanent teeth were infected with Enterococcus faecalis. After incubation at 37°C for 24 h, three hundred teeth were instrumented with three different instrumentations using two irrigation methods. The remaining ten teeth were used as negative and positive control groups, in which no inoculation was done and no instrumentation was carried out respectively. Three hundred teeth were equally divided in three groups (n = 100), in which instrumentation was performed using a protaper universal rotary file (group 1), WaveOne reciprocating file (group 2) and a flexiCON rotary file (group 3). In each group, 50 samples were irrigated with conventional needle irrigation, and 50 samples were irrigated with the endoVac irrigation method. During instrumentation, apically extruded bacteria were collected in an Eppendorf tube. Microbiological samples were taken from the Eppendorf tube and incubated for 24h, and colony-forming units were counted. The data collected were statistically analysed. Results: The group 2 showed highest bacterial extrusion using conventional irrigation while group 3 showed lowest using endovac irrigation system. Conclusion: FlexiCON rotary instrumentation with the endoVac irrigation system produced significantly less bacterial extrusion than the other techniques.

Effectiveness of different rotary file systems in removing the root canal filling material: A micro-computed tomography study.

Background. This study aimed to examine the retreatment efficiency of four NiTi rotary file systems with micro-computed tomography (micro-CT). Methods. Forty premolar teeth were shaped up to F2 using the ProTaper Universal rotary file system and obturated with MTA Fillapex. The root canals were scanned with micro-CT to evaluate the volume of root canal filling before retreatment. The samples were randomly divided into four groups according to the file system used to remove root canal filling material (n=10): ProTaper Universal Retreatment, ProTaper NEXT, EdgeFile XR, and EdgeFile® X3 NiTi system. All the samples were scanned with a micro-CT device for the second time to evaluate the amount of residual filling material in the root canals. The percentages of filling material removed from root canals were calculated. Additionally, the time spent on the removal of the root canal filling material was recorded. The data were analyzed using the Shapiro-Wilk and Kruskal-Wallis tests. Results. There were no significant differences between the groups in the percentage of root canal filling material removal. However, a statistically significant difference was found between the groups in the time required to reach the apex and remove the entire filling material. The time required to remove the root canal filling material was higher in the EdgeFile® X3 group. Conclusion. NiTi files manufactured for root canal preparation can be used effectively and safely to remove root canal filling materials. EdgeFile XR produced for retreatment can be used as an alternative to ProTaper Universal Retreatment files.

INCIDENCE OF APICAL ROOT CRACK FORMATION AFTER ROTARY ROOT CANAL INSTRUMENTATION AT DIFFERENT INSTRUMENTATION LENGTHS USING PROTAPER UNIVERSAL, PROTAPER NEXT AND PROTAPER GOLD FILES - AN IN-VITRO STUDY

Aim: To evaluate the incidence of apical root crack formation after root canal preparation using ProTaper Universal (PTU), ProTaper Next (PTN) and ProTaper Gold (PTG) rotary file systems and compare the crack formation at various instrumentation lengths. Subjects and Methods: One hundred single rooted extracted mandibular premolar teeth were mounted in acrylic resin blocks after simulating periodontal ligament. The teeth were divided into four groups. Group I was instrumented using PTU, group II using PTN and group III using PTG rotary files, while group IV was left untreated, serving as a negative control group. Each group was subdivided into three subgroups: A, B and C instrumented till root canal length (RCL), RCL-1 and RCL+1. Root apex was sectioned horizontally 1-2mm from apical foramen and was stained with 1% methylene blue dye followed by stereomicroscopic evaluation to determine apical root cracks. The data was analyzed using chi-square, Shapiro Wilkinson and Cramers phi test. The significance level was set at P&lt;0.05. Result: Significant difference was seen in percentage of cracks after instrumentation with PTU and PTG, while no significant difference with PTU and PTN. Specimen instrumented upto RCL-1 showed less cracks as compared to those instrumented upto RCL and RCL+1. Conclusion: PTG produced least number of cracks followed by PTN and PTU. Moreover, instrumentation at RCL-1 reduced the crack formation.

Apical Leakage Evaluation of Two Different Coated Carrier Systems for Root Canal Obturation Using a Dye Penetration Evaluation Method

The carrier-based obturation is a reliable technique for sealing the endodontic space. The GuttaCore with a pink internal core of cross-linked gutta-percha, named GuttaCore Pink, has been recently introduced into the market. The aim of this in vitro study was to compare the quality of the apical seal of two-carrier-based system, Soft Core and the GuttaCore Pink, through the measurement of apical dye leakage. Eighty-six extracted human teeth with single canals were used. Samples were shaped using ProTaper Universal rotary files up to a #30 apical size and randomly divided into four groups. Group SC (n = 40) was obturated with #30 Soft Core Obturators; Group GCP (n = 40) was obturated with #30 GuttaCore Pink obturators; Group CT+ (n = 3) and CT- (n = 3) left nonobturated used as positive and negative controls, respectively. The samples underwent a process of passive/active dye penetration and after a clearing procedure. The extent of the dye was measured under stereomicroscope. The Mann-Whitney U test showed a statistically significant difference (p <0.05) between Soft Core and GuttaCore Pink in terms of apical dye leakage, both considering the mean and the maximum infiltration value with a greater infiltration rate for Soft Core. In vitro GuttaCore Pink showed less apical dye leakage than Soft Core. The apical leakage of carrier-based obturation materials, observed in both GuttaCore Pink and Soft Core, may be considered material-dependent.

Efficiency of Supplementary Contemporary Single-file Systems in Removing Filling Remnants from Oval-shaped Canals: An In Vitro Study

This study investigated the performance of supplementary three single-file systems for eliminating root filling residue from oval-shaped canals utilizing cone-beam computed tomography (CBCT). Thirty single-rooted human mandibular premolars with oval-shaped canals were chosen. After decoronation and working length determination, ProTaper Universal (PTUR) rotary files were used to prepare canals up to F3. Irrigation was fulfilled by 2.5% sodium hypochlorite (NaOCl), 17% ethylenediaminetetraacetic acid (EDTA), and lastly distilled water. By utilizing the lateral compaction technique, canals were filled by gutta-percha and Endosequence BC Sealer. The PTUR retreatment system (D1, D2, and D3) eliminated the primary filling material sequentially. Subsequently, specimens were categorized into three groups at random: group I: WaveOne Gold (WOG) primary file, group II: TruNatomy (TRN) medium file, and group III: XP-endo Finisher R (XPF-R). The volume of residual left from filling material was quantified utilizing CBCT imaging before and after the supplementary retreatment. Statistical analyses were done by one-way analysis of variance (ANOVA) and Tukey's test at a 5% significance level. The volume of removed root filling after using XPF-R was significantly higher than using WOG in all sections and TRN in the coronal third, while nonsignificantly higher than using TRN in the apical and middle. XPF-R as a supplementary technique revealed the highest percentage reduction of root filling material (73.13%) in comparison with the other systems (37.39% WOG and 44.16% TRN) in oval-shaped canals. Neither one of the methods and tools used to remove filling material from oval-shaped root canals is perfectly efficient. The performance of supplementary techniques using contemporary single-file systems improves the cleaning and reshaping of root canal space that permits a proper disinfection to optimize the outcome of retreatment option.

Evaluation of debris and smear layer formation using three different NI-TI rotary instrument systems: An in vitro scanning electron microscope study.

Aim: The aim of the study is to evaluate of debris and smear layer formation after using rotary ProTaper Universal, Twisted File, and XP Endo file systems under scanning electron microscope.Materials and Methods: Forty freshly extracted mandibular second premolar teeth were taken to decoronate at the cementoenamel junction to make the remaining root length 15 mm. Specimens were divided into four groups of 10 teeth each, Group I (control) – no instrumentation. Group II – ProTaper Universal rotary file (F2), Group III – twisted file (ISO size 0.25 and 6% taper), Group IV – XP Endo file (ISO size 0.25). During instrumentation, 5 ml normal saline was used as irrigating agent. Grooves parallel to the longitudinal axis of the root were made on the mesial and distal surface of each specimen to split it into two halves and examined under scanning electron microscope at ×1500 and ×5000 magnification. Photomicrographs were taken to evaluate debris and smear layer. Evaluation of photomicrographs was done using a score index.Results: One-way analysis of variance (ANOVA) was used to compare more than one means at a time. Tukey's critical difference followed by ANOVA was used to compare the mean values pair wise. P <0.05 was considered to be statistically significant. Among all the file systems, Group II showed maximum amount of debris (3.50 ± 1.109) followed by Group III (2.83 ± 1.238) and least amount was showed by Group IV (2.65 ± 1.122) at all levels (cervical, middle, and apical third). Among all the experimental groups, Group II showed maximum amount of smear layer (2.75 ± 1.149) followed by Group III (2.40 ± 0.982) and least amount of smear layer shown by Group IV (2.10 ± 0.841) at all levels (cervical, middle and apical third), the result was statistically significant (P < 0.05).Conclusions:At all the levels (cervical third, middle third, and apical third), among all the experimental groups, highest amount of debris and smear layer was formed by ProTaper Universal rotary file followed by Twisted file and least amount showed by XP Endo file system. In all the levels, control group showed highest amount of debris but least amount of smear layer.

Sealer penetration in the dentinal tubules: A confocal laser scanning microscopy study

Context: The penetration of sealer into dentinal tubules is considered to be a desirable feature as sealers prevent the colonization of residual bacteria into the dentinal tubules because of their antibacterial properties.Aims: This study aims to compare the depth of penetration of ADSEAL, Sealapex, and BioRoot RCS into the dentinal tubules in the apical area using confocal laser scanning microscope (CLSM).Settings and Design: Quality improvement projects.Materials and Method: After decoronating 120 extracted single rooted teeth, preparation was done using ProTaper universal rotary files until F3. Then, the samples were divided into four groups (n = 40) and obturated by the following endodontic sealers: Group A-ADSEAL, Group B-Sealapex and Group C-BioRoot RCS along with F3 gutta-percha cone. Before root canal filling, the sealers were mixed with 0.1% Rhodamine B dye. All the samples were sectioned at 3 mm and 5 mm from apex and visualized under CLSM.Statistical Analysis Used: Data were analyzed using two-way ANOVA and Tukey post hoc tests.Results: BioRoot RCS has shown significantly higher depth of penetration than resin based sealer and calcium hydroxide based sealer (α < 0.05).Conclusion: At all root regions, BioRoot RCS exhibited more tubular penetration whereas ADSEAL exhibited less penetration.

Evaluation of the Effect of Enlargement of the Apical One Third by Protaper Universal Rotary Files System on Elimination of Enterococcus faecalis and Surface Irregularities in Single Canaled Premolars (A ComparativeIn-Vitro Study)

Evaluation of the Effect of Enlargement of the Apical One Third by Protaper Universal Rotary Files System on Elimination of Enterococcus faecalis and Surface Irregularities in Single Canaled Premolars (A ComparativeIn-Vitro Study)

In vitro assessment of natural extract as irrigant on root dentin microhardness

To determine the effect of a natural extract of of 2.5% concentration on the of root canal when used as an irrigating solution. This was an in comparative study where and twenty intact single-rooted teeth were selected and with a diamond bur to get an apical-coronal length of 10 mm and were randomly separated into three groups as per the used; Group 1 (control): Irrigation with Normal saline, Group 2: Irrigation with Smear Clear solution, Group 3: Irrigation with 2.5% extract solution. were prepared using Pro Taper Universal Rotary Files with intermittent irrigation with the respective irrigating solution. The teeth were then embedded in acrylic resin and subjected to Vicker's Hardness test, and the data obtained were analyzed using one way ANOVA. When Group 2(51.80±9.80 VHN and 52.66±5.28 VHN) was compared with Group 3(44.98±4.34 VHN and VHN) the values showed a statistically significant difference (p&lt; 0.05).When Group 3 (44.98±4.34 VHN and VHN) was compared with Group 1(65.37±8.80 VHN and 67.74±9.56 VHN), the values did show a statistically significant difference (p&gt; 0.05). Within the limitations of this study, teeth irrigated with normal saline showed the least reduction in followed by irrigation with leaf extract solution and Smear Clear.

Comparison of dentin penetration ability of different root canal sealers used with different obturation methods.

This in vitro study aimed to investigate the tubular penetration depth of four different sealers used with two different obturation methods in the apical, middle, and coronal root canal dentin. Eighty-four single-rooted teeth were instrumented until the F4 ProTaper universal rotary file and filled with AH26, AH Plus (Dentsply, DeTrey, Konstanz, Germany), RealSeal (SybronEndo, Orange, CA), and MTA Fillapex (Angelus, Londrina, Brazil) using cold lateral condensation (CLC) and single-cone (SC) obturation techniques. Thereafter, the roots were sectioned longitudinal and prepared for scanning electron microscope evaluation. The maximum and minimum sealers penetration depth into dentinal tubules at the apical, middle, and coronal regions and the average depth of sealer penetration were calculated. The data was statistically analyzed two-way ANOVA and post hoc Tukey and Dunnett tests and independent samples t test using maximum mean values. There was no statistically difference between the obturation methods (p > .05). When the sealers are evaluated within themselves AH26 was affected by obturation methods significantly (p < .05). Also the results revealed a significant difference between root regions (p < .05). AH26 and RealSeal root canal sealers had the highest penetration values with CLC and SC obturation techniques, respectively. The obturation method did not affect the penetration amount of root canal sealers. RealSeal root canal sealer performed better penetration ability than the other sealers. Apical third of the root in all groups showed lowest penetration depth.

Laboratory Rational of Changes in the Crystal Lattice of Nickel-Titanium Endodontic Rotary Files in Autoclaving

Objectives The aim of this study was to investigate the changes of the crystal lattice of NiTi instruments after repeated autoclave sterilization cycles based on the results to conclude about the influence of multiple sterilization on the characteristics of ProTaper clinical use. Methods 21 samples of ProTaper Universal rotary files were divided into 3 groups of 7 samples. After 1, 4, and 7 cycles of sterilization, the samples were observed using еnergy-dispersive X-ray spectroscopy (EDX), X-ray powder diffraction (XRD), and scanning electron microscopy (SEM). Results EDS analysis confirmed that all samples were composed mainly of nickel and titanium, also Fe (presumably steel), Cr, Co, and Zn were found. The percentage of nickel and titanium was affected by repeated sterilization cycles. The mass fraction of Fe, Co, Zn, and Cr decreased after 1, 4, and 7 sterilization cycles. According to the results of the second study, it was found that, with increasing autoclaving cycles, the percentage of Fe decreased. There were changes in the three obtained diffractograms, which indicated an increase in the number of the martensitic phase and a decrease in austenitic. The cutting efficiency of the ProTaper is also reduced during repeated sterilization cycles, which can be caused by the austenitic and martensitic phase displacement. Conclusion Manufacturing features and repeated sterilization cycles increase the internal deformation in the structure of NiTi alloy, which increases the risk of fracture.