Proportion Of White Clover Research Articles

УСТОЙЧИВОСТЬ КЛЕВЕРА ПОЛЗУЧЕГО (TRIFOLIUM REPENS L.) В СЕЯНЫХ ТРАВОСТОЯХ В ЗАВИСИМОСТИ ОТ ИХ СОСТАВА, КРАТНОСТИ СКАШИВАНИЯ И АЗОТНЫХ УДОБРЕНИЙ

The authors carried out studies at the experimental field station of Russian State Agrarian University – Moscow Timiryazev Agricultural Academy in 2008–2020. The study aimed to determine the resistance of white clover in mixtures with various types of grasses with two- and three-cut clipping treatments. In the experiment with four to five-component mixtures, the proportion of white clover reached 36–53% in the botanical composition of swards with the content of perennial ryegrass in the fourth year of life. It did not exceed 22–23% in the mixtures with cock’s foot. With the application of nitrogen fertilizers at a dose of N180, the clover content in the second year of swards was only 1–27%. With the aging of the swards, the proportion of clover in the harvest decreased. In the ninth year of life, its amount in swards with the content of perennial ryegrass without nitrogen application decreased to 13–24%, and in agrophytocenoses with cock’s foot and with nitrogen application to 0–14%. The cock’s foot dominated in the four-component grass mixtures, but during the overwintering of 2015–2016, cock’s foot and perennial ryegrass almost entirely dropped out of agrophytocenoses. Tall fescue was a more resistant species. The yield of grass mixtures composed of grass varieties of Russian selection amounted to 4.16–5.52 t/ha of dry weight on average for eight years, and from varieties of Dutch selection, the yield equaled 4.07–5.24 t/ha. There were no significant differences in yield between grass-legume mixtures based on perennial ryegrass and cock’s foot. With the introduction of nitrogen in a dose of N180 on grass/legume herbage, the yield increased by only 31%, and the return on fertilizers by yield increases was low, making up 4.6–8.9 kg of dry matter per 1 kg of nitrogen. The aftereffect of nitrogen fertilizers on the yield of grass-legume mixtures without the additional introduction of phosphorus and potassium was insufficiently effective. In long-term three-component swards, used from the 14th to the 24th years of life, white clover was more stable in the composition of agrophytocenoses after three-fold mowing than with two-mowing. In years with favorable atmospheric moisture conditions, the proportion of white clover in two and three mows reached 20.1–34.7 and 2.2–24.3%, respectively, and with a moisture deficit, it decreased to 0–9%. For 26 years of growing grasses, there was a significant increase in soil acidity – pHKCl decreased from 6.3 to 4.91–5.36, which negatively affected the resistance of clover as the herbage was aging.

Implications of grass–clover interactions in dairy pastures for forage indexing systems. 3. Manawatu

ABSTRACTThe implementation and monitoring of the treatments in the core experiment (eight perennial ryegrass cultivars grown under four combinations of plus/minus clover and high/low nitrogen (N)) at a dryland Manawatu site are described. Herbage accumulation (HA) was significantly greater for high N than for low N in 14 out of 21 annual and seasonal totals. There were no significant main effects of clover and there were no interactions between clover and N level. With the exception of autumn 2016, there were significant differences among ryegrass cultivars in all seasons, but no clover × cultivar or N × cultivar interactions for HA or nutritive value (NV). With the caveat that successive droughts reduced the proportion of white clover to low levels, the hypothesis that relative HA or NV rankings of ryegrass cultivars do not differ when ryegrass is grown in monoculture or in mixtures with white clover is supported.

Associative effects between fresh perennial ryegrass and white clover on dynamics of intake and digestion in sheep

AbstractThe aim of this study was to determine the associative effects between fresh perennial ryegrass and fresh white clover on intake and digestion in sheep. Five proportions of ryegrass and white clover, ranging from 0 to 1 in 0·25 increments (DM basis), were allocated to two groups of five sheep according to a replicated 5 × 5 Latin square design, and intake behaviour and digestive parameters including methane (CH4) emissions were measured. Feeding behaviour was affected through increased forage intake when the proportion of white clover was increased, and there were positive associative effects on intake, eating time and eating rate during the main meals consumed immediately after feed was given. These effects seemed to be related to a greater motivation to eat, rather to more efficient digestion, as no difference on DM digestibility (average 766 g kg−1) was detected. There were no differences in CH4 yield (average 18·1 g kg−1 DM intake) among the treatments. The optimal proportion of white clover fed with ryegrass to optimize intake and digestion was between 0·25 and 0·50. These results indicate that mixing perennial ryegrass and white clover positively affects forage intake in sheep and their motivation to eat.

Changes of soil agrichemical characteristics in pastures influenced by mineral fertilizing

Grasslands fertilizing is used for increasing of forage yields and quality and simultaneously for soil fertility maintenance. The aim of this study was to evaluate the effect of mineral fertilizing on basic agrichemical soil characteristics (pH, available nutrients) and soil organic matter at grazing exploitation of grasslands. 60 kg N.ha−1 was applied in the year of experiment establishment and 90 kg N, 30 kg P a 90 kg K ha−1 in each of following four years. Significantly higher pH (+0.17), available P (+48.5%), K (+15.4%) and organic C (+8.8%) content was found at fertilized treatment. pH value varied significantly during experiment period (range 0.6) and correlated inversely with proportion of white clover in first cut (r = −0.499). Although available K in soil was only on satisfactory level (less than good), K concentration in the forage was even from unfertilized plots higher than physiological requirement of plants. Available nutrient content constitutes only small proportion of total nutrient content: 5.1% at P, 2.1% at K, 12.0% at Mg and 39.0% at Ca.

N 2-fixation and residual N effect of four legume species and four companion grass species

Inclusion of forage legumes in low-input forage mixtures improves herbage production and soil fertility through addition of nitrogen (N) from N 2-fixation. The impact of different grass–legume mixtures on the N contribution of the forage mixture has rarely been investigated under comparable soil and climatic conditions. We conducted a field experiment on a sandy soil at two nitrogen levels with seven two-species forage mixtures: alfalfa, bird's-foot trefoil, red clover, or white clover in mixture with perennial ryegrass, and white clover in mixture with meadow fescue, timothy, or hybrid ryegrass. We found high N 2-fixation of more than 300 kg N ha −1 from both red clover and alfalfa even when the two mixtures received 300 kg total-N ha −1 in cattle slurry. The addition of cattle slurry N fertilizer lowered N 2-fixation for white clover and red clover as expected, but for bird's-foot trefoil and alfalfa no changes in the proportion of N derived from N 2-fixation was observed. We conclude that the competition for available soil N from perennial ryegrass in mixture was an important factor for the proportion of N in alfalfa, white clover, and bird's-foot trefoil obtained from N 2-fixation. White clover had a high proportion of N derived from atmosphere for all companion grasses despite significant differences in white clover proportion. Although the perennial ryegrass–alfalfa mixture in the grass phase yielded more than twice the N from N 2-fixation compared to white clover in the perennial ryegrass mixture, this did not in the following year lead to higher residual N effects of alfalfa. Both in terms of N yield in the grass phase and N yield in the subsequent spring barley red clover contributed most to the improvement of soil N fertility.

Improving the effectiveness of wood‐ash fertilization in mixed mountain pastures

AbstractMountain pastures can be improved by the application of wood ash, but its effectiveness as a source of N, P and other elements requires improved management practices. Two application rates of wood ash (6 and 12 t ha−1) were tested in a 4‐year trial on a low‐input fertilized pasture. Ash was complemented with mineral P, and N fixation was encouraged by sowing white clover. Wood‐ash application led to increased pasture yield (by 100%) and to increased proportions of white clover and ryegrass (60%), thus improving pasture feed value. N fixation by white clover, and possibly increased mineralization because of higher pH, may have compensated for lack of N in the wood ash. Addition of mineral fertilizer improved the supply of P, although incorporation of the wood ash into the soil may also have enhanced release of P from non‐extractable components. High amounts of compounds containing Ca and Mg contributed to the long‐term maintenance of soil reserves of these elements. Despite high inputs of K (175–350 mg kg−1) from wood ash, annual additions of K are required to counteract depletion via plant uptake and leaching. Increased Mn availability after the treatment (up to 400 mg kg−1) may limit repeated applications of wood ash, at least on poorly aerated soils.

Comparison of meat and carcass quality in organically reared and conventionally reared pasture-fed lambs

The ‘Organic’ product label guarantees a production process that avoids the use of synthetic fertilisers, pesticides and hormones and minimises recourse to pharmaceuticals or veterinary drugs; however, the product's quality remains an issue that needs to be addressed in response to consumer demand. Consequently, this study was conducted to compare the sensory and nutritional qualities of meat and carcasses from pasture-fed lambs reared organically (O) or conventionally (C). Mean lamb growth profile was kept similar between the two treatments to avoid confounding effects with lamb age or weight at slaughter. The experiment was conducted over 3 years (2005 to 2007) with 12 O and 12 C lambs each year. The O and C treatments differed in the level of on-pasture mineral N fertilisation inducing a higher proportion of white clover in the organic pasture than the conventional pasture. Lambs were slaughtered when they attained a fat class of 2 to 3, and carcass and meat quality were evaluated. Lambs were slaughtered at an average weight and age of 35.3 kg and 156 days in the O treatment, respectively, and 35.2 kg and 155 days in the C treatment, respectively. Sensory evaluation indicated that loin chops from the O treatment had a higher level of abnormal fat odour compared with the C treatment. Carcasses from the O treatment had a softer subcutaneous fat one among 3 years (2007) compared to the C treatment. These results are probably due to a higher proportion of white clover in the diet. Organically reared lambs did offer the slight advantage of muscle fatty acid containing a higher level of stearic acid, which may have positive effects in the prevention of cardiovascular disease in humans. This may be the result of a higher rumen bio-hydrogenation of C18:3n-3 due to differences in the botanical composition between the O and the C pasture. Production system had no effect on the colour characteristics of the meat and subcutaneous fat, except lightness of subcutaneous dorsal fat, which was slightly higher in the O lambs. There were no differences between O and C lambs in terms of colour stability and lipid oxidation of the meat during the 6-day refrigerated storage under gas-permeable film.

Effect of grazing fresh legumes or feeding silage on fatty acids and enzymes involved in the synthesis of milk fat in dairy cows

The impact of fresh legume types or silage on the composition of milk fatty acids and transcription of enzymes involved in the synthesis of milk fat in cows was studied. Three groups of cows grazed high proportions of white clover, red clover and lucerne, respectively. A fourth group of cows was fed maize/grass silage. The cows grazing high proportions of legumes produced significantly more 18:1 trans-11, 18:2 cis9-trans11, 18:2 trans10-cis12 and 18:3 fatty acids than cows fed silage. White clover and lucerne grazing resulted in significantly lower output of 18:1 trans9 in milk than red clover grazing and maize/grass silages. Transcription of stearoyl-CoA desaturase (SCD) in mammary tissue was significantly increased by grazing high proportions of legume whereas fatty acid synthase and acetyl-CoA carboxylase were not affected by type of feeding. Furthermore, average milk fat globule diameter was correlated to daily milk fat yield but was not affected by feeding. Although the fresh forage affected the transcription of SCD in mammary tissue, the largest effects were on the trans11-based fatty acids. It is concluded that type of forage, i.e. fresh or silage, had a greater impact on rumen fermentation pattern than on transcription of enzymes involved in the synthesis of milk fat.

Interactions of slope and canopy of herbage of three herbage species on transport of faecal indicator bacteria by rain splash

AbstractThe movement of faecal pathogens from land to surface and ground‐water are of great interest because of the public and livestock health implications. Knowledge of canopy structure and how it might be managed to help mitigate nutrient and pathogen movement in pasture is needed to create management practices that balance livestock production with environmental benefits. An experiment was conducted using a rainfall‐simulating device to test whether canopy structure of species common to pastures in Appalachia, USA could be managed to influence dispersion of faecal pathogens. Seven pots (30‐cm diameter) of white clover, orchardgrass and perennial ryegrass were lined up on horizontal and sloping surfaces under a rainfall simulator. The centre pot was inoculated at the soil surface with 4 × 1010 faecal coliform bacteria (FC) just before rainfall simulation started. The species were maintained under short, moderate and tall canopy management treatments. White clover exhibited the greatest rates of lateral and vertical dispersion of FC into the canopy, especially in the short canopy management treatment following 30 min of rainfall (about 40 mm). Low concentrations of faecal coliform bacteria also dispersed into the canopies of the grass species but the differences in concentration of FC between the grass species were not different. When the proportion of white clover in a pasture is high, the canopy should be relatively taller to reduce the likelihood of infection associated with faecal coliform‐contaminated herbage.

Effect of surface applied glycine betaine on herbage production and quality of perennial ryegrass - white clover pastures

Osmoprotectants have been reported to reduce the detrimental effects of various environmental stresses in many different plant species. However, there is little research available concerning pasture species. Two experiments were undertaken with the aim of quantifying the effect of surface applications of exogenous glycine betaine (GB) on herbage production and quality of perennial ryegrass (Lolium perenne L.)–white clover (Trifolium repens L.) pastures during periods of moisture stress and cold temperatures over 2 years. Pastures fertilised with GB were compared with unfertilised pastures and pastures fertilised with nitrogen (N). Rates of 0.5, 1.0 and 1.5 kg GB/ha.defoliation were applied in experiment 1 and 5 kg GB/ha.defoliation was applied in experiment 2. Surface applications of GB did not significantly affect herbage production relative to unfertilised pastures; herbage yields averaged 12 248 and 12 693 kg DM/ha over 11 months in experiment 1, and 7253 and 7177 kg DM/ha over 6 months during summer and autumn in experiment 2, for the unfertilised control and GB, respectively. During both experiments, herbage quality parameters were not affected by GB application, although the proportion of white clover in the sward between summer and winter during experiment 1 was greater (P < 0.01) in plots treated with GB than in untreated plots. Application of N fertiliser increased (P < 0.001) herbage production, but did not consistently affect herbage quality. The failure of surface applications of exogenous GB to improve the herbage production or quality of perennial ryegrass–white clover pastures suggests that it is not an appropriate method to enhance plant tolerance to environmental stress at the concentrations applied in these studies.

Herbage intake, milk production and sward utilization of dairy cows grazing grass/white clover swards at low, medium and high allowances

Herbage intake (HI), milk production and sward utilization of Norwegian Red Cattle dairy cows in mid-lactation, rotationally grazing grass/white clover swards at low, medium and high allowances (HA), were examined in three experiments (I, II and III). The daily target allowances per cow were 18 and 36 kg DM in Experiment I and 12, 18, and 24 kg DM in Experiments II and III. The cows were grazing in groups of seven (Experiments II and III) to ten (I) individuals. The proportions of white clover in the swards averaged 12%, 40% and 46% in Experiments I, II and III, respectively. In addition to pasture, the cows were fed concentrate mixtures in varying amounts (0–6.8 kg) with the highest amounts offered to the highest yielding cows. HI was estimated using the n-alkane technique. No effect of HA on HI was obtained in Experiment I. In Experiment II, and for II and III combined, there was a significant positive effect of HA on HI. The average increase in HI was 0.24 kg DM per extra kg DM on offer. The utilization of the sward (measured at 3 cm above ground level) decreased from 72% at the lowest HA to 51% at the highest HA in Experiment II and III. HA did not affect milk yield or milk composition significantly (P>0.05).

Effects of condensed tannins in white clover flowers on their digestion in vitro

Protein in white clover ( Trifolium repens L.) is poorly utilised by ruminants because of its extensive degradation to ammonia in the rumen. However, white clover produces condensed tannins (CT) in its flowers, which can reduce rumen proteolysis. Effects of increasing proportions of clover dry matter (DM) as flowers (and therefore floral CT) on soluble protein, ammonia and volatile fatty acid (VFA) concentrations were determined with in vitro incubations. Minced mixtures of 0, 250, 500, 750 and 1000 g/kg of DM as white clover flower (F) with the remainder as white clover leaf, were incubated in vitro and sampled after 0, 2, 4, 8, 12 and 24 h. Treatments contained 0, 13, 26, 39 and 52 g CT/kg DM, respectively. A further treatment with 500 g/kg DM as flower and 500 g/kg DM as leaf had polyethylene glycol added to remove effects of CT. Increasing the proportion of white clover as flowers from 0 to 1000 g/kg DM reduced net conversion of plant N to ammonia N from 290 to 120 mM/M at least partly due to reduced solubility of the protein. Treatments with 750 g/kg DM or more as clover flowers reduced ammonia concentrations to levels likely to limit microbial growth. Total VFA production was not affected by flower content, although the proportion of acetate to propionate increased. The contribution of CT to treatment effects was small compared to effects attributed to difference in chemical composition between flowers and leaves.

Using n-alkanes to estimate diet composition of herbivores: a novel mathematical approach

N-alkanes are long-chain saturated hydrocarbons occurring in plant cuticles that can be used as chemical markers for estimating the diet composition of herbivores. An important constraint of using n-alkanes to estimate diet composition with currently employed mathematical procedures is that the number of markers must be equal or larger than the number of diet components. This is a considerable limitation when dealing with free-ranging herbivores feeding on complex plant communities. We present a novel approach for the estimation of diet composition using n-alkanes which applies equally to cases where the number of markers is lower, equal or greater than the number of plant species in the diet. The model uses linear programming to estimate the minimum and maximum proportions of each plant in the diet, and avoids the need for grouping species in order to reduce the number of estimated dietary components. We illustrate the model with two data sets of n-alkane content of plants and faeces obtained from a sheep grazing experiment conducted in Australia and a red deer study in Portugal. Our results are consistent with previous studies on those data sets and provide additional information on the proportions of individual species in the diet. Results show that sheep included in the diet high proportions of white clover (from 0.25 to 0.37), and relatively high proportions of grasses (e.g. brome from 0.14 to 0.26) but tended to avoid Lotus spp. (always less than 0.04 of the diet). For red deer we found high proportions of legumes (e.g. Trifolium angustifolium and Vicia sativa reaching maximum proportions of 0.42 and 0.30 of the diet, respectively) with grasses being less important and Cistus ladanifer, a browse, also having relevance (from 0.21 to 0.42 of the diet).

The effects of treading by dairy cows during wet soil conditions on white clover productivity, growth and morphology in a white clover–perennial ryegrass pasture

AbstractSingle pugging events, which involve remoulding of the soil around the hooves of livestock during treading, of moderate or severe pugging intensity were imposed in plots in a long‐term white clover‐ryegrass pasture during spring, by using dairy cows at varying stocking rates (4·5 cows 100 m−2 for 1·5 or 2·5 h respectively). Changes in the growth and morphology of white clover were investigated over the following 12 months. Defoliation at approximately 3‐week intervals was carried out by mowing. Annual herbage production was reduced following moderate and severe pugging proportionately by 0·16 and 0·34 compared with the non‐pugged control treatment. The corresponding decreases in white clover production were 0·09 and 0·52 respectively. Annual perennial ryegrass production was reduced by 0·37 under severe pugging. Pugging had an immediate adverse effect on growth of white clover which persisted for up to 156 d, and coincided with a large decrease in the proportion of white clover in herbage over the same period (e.g. 0·40 vs. 0·12, in control and severely pugged treatments, respectively, on day 112). In comparison, recovery in ryegrass growth was apparent after 50 d in severely pugged treatments, indicating that white clover is more vulnerable to severe pugging than perennial ryegrass. Analysis of individual white clover plants extracted from turves (300 mm × 300 mm) showed that direct hoof damage, fragmentation and burial of stolons were the major factors which reduced white clover production, rather than the changes in soil physical properties measured. Morphological characteristics associated with plant size (e.g. stolon length, growing points, and leaf numbers) all decreased under pugging. The situation had reversed by late summer, with larger plants dominating pugged plots, and coincided with the recovery of the proportion of white clover in herbage. Strategic pasture management practices, such as restricted grazing and the use of stand‐off pads when soils are overly wet, are suggested as means of minimizing treading damage to pasture and reducing negative impacts on the growth and productivity of white clover.

Diagnosing phosphorus status of natural grassland in the presence of white clover

The phosphorus nutrition index (PNI) method proved to be suitable for diagnosing P nutrition level over a wide range of grass swards. It is based on relative nutrient concentrations and relies on a critical curve for optimum P concentration which is a function of sward N concentration. The objectives of this paper were to verify to what extent this method was valid for P nutrition diagnosis when white clover was present in the sward in view of the fact that white clover N concentration is frequently reported to be much higher than that of grass and to remain constant whatever the amount of biomass produced. For this purpose we analysed the effect of N addition on N and P concentrations in white clover and rye grass grown in pure stands with unlimited P supply in a garden collection and compared it with clover behaviour in a field experiment on a natural grassland with a similar P status. Clover displayed a different affinity for P according to whether it grew in a pure or mixed sward. In a pure stand, we observed a very significant effect of N on N and P concentrations measured in individual organs and whole plants; the highest N concentrations were measured in clover. This species behaved differently from ryegrass: for a given level of N supply, plant N concentration decreased when petioles were added to the laminae samples, while P concentrations remained constant; for rye grass both N and P concentrations decreased when sheaths were added to laminae. Clover in mixed swards had a lower P nutritional status than when grown in a pure stand, due to its lower capacity to compete with grasses for light capture and P acquisition. Analysis of data from the literature demonstrated that differences in response between clover and grass were more pronounced under limited P supply, when clover displayed a lower ability than grasses to adjust P concentration to that of N due to its lower capacity to adapt to low P availability. As a consequence it became even less competitive. We concluded that there are limitations to the use of nutrient diagnosis systems based on relative concentrations when the herbage contains a large proportion of white clover and proposed that only the non-clover fraction should be used for diagnosis to avoid excessive P application when PNIs are used for fertiliser recommendations.

Methane emissions by dairy cows fed increasing proportions of white clover (Trifolium repens) in pasture

Methane (CH4) production from ruminant digestion has a significant impact on the New Zealand greenhouse gas (GHG) inventory and represents a loss of about 10% of metabolisable energy (ME) intake. Previous trials with sheep and cattle have demonstrated significantly lower methane losses per unit feed intake from legumes compared to grass dominant pasture. Most trials have compared forages fed as sole diets but white clover (Trifolium repens) is usually fed with perennial ryegrass (Lolium perenne) as a mixed pasture, as it complements ryegrass for animal production. An indoor feeding trial was conducted in December 2003 with thirty-two Holstein-Friesian dairy cows in mid-lactation to determine effects of increasing proportions of white clover on methane emissions and cow performance. Cows were housed indoors and fed perennial ryegrass with 0, 15, 30 or 60% white clover ad libitum on a dry matter (DM) basis. Increasing proportions of white clover resulted in linear increases in dry matter intakes (DMI) and reductions in methane per kg DM eaten, although the extent to which CH4 production/kg DMI was lowered was less than anticipated from previous measurements from sheep fed white clover as a sole diet. DMI of cows fed 60% white clover was 20.5 vs. 15.6 kg DM/cow/day for 100% perennial ryegrass (P

The effect of pasture species on parasitism and performance of lambs grazing one of three grass—white clover pasture swards

Lamb parasitism and performance were compared on three pasture treatments: browntop/white clover, Yorkshire fog/white clover, and ryegrass/white clover swards maintained at a sward height of 5 cm in each of two years by using continuous variable stocking management. In year 1, lamb performance (both suppressively drenched (SD) and trigger drenched (TD)) was similar on ryegrass and browntop swards but was poorer ( P<0.05) on Yorkshire fog swards. In year 2, lamb performance (both SD and TD) was markedly better on ryegrass than on Yorkshire fog or browntop swards. This coincided with an increase in white clover content in the ryegrass/white clover swards and a decrease in white clover content in the browntop/white clover swards. Lamb performance was related to the white clover content of the swards ( R 2=85.4, P<0.001 and R 2=77.5, P<0.001 for SD lambs and TD lambs, respectively). Differences among pasture treatments in faecal egg count (FEC) of TD lambs were greater in year 2 than in year 1 when no significant pasture effects were observed. In year 2, FEC was lowest in lambs which grazed ryegrass/white clover, intermediate in those which grazed Yorkshire fog/white clover and highest in lambs which grazed browntop/white clover, despite herbage allowance (kg herbage mass/kg lamb mass) being greater on the browntop/white clover and Yorkshire fog/white clover swards than the ryegrass/white clover swards. Circulating antibodies to gastrointestinal parasites were measured at the end of year 2. Antibody titres were highest ( P<0.05) in the plasma of lambs which grazed ryegrass/white clover swards, and lowest in lambs which grazed browntop/white clover swards due to differences in levels of antibody to ( P<0.05) Haemonchus contortus, Ostertagia circumcincta larvae and adults, but not Trichostrongylus colubriformis larvae or adults. These findings indicate that pasture composition, especially, the proportion of white clover, can help alleviate the production losses due to gastrointestinal parasitism. This information can provide a quick and readily adoptable practice for farmers who aim to reduce drench reliance and could become a key component of any integrated parasite control programme.

The implications of controlling grazed sward height for the operation and productivity of upland sheep systems in the UK. 5. The effect of stocking rate and reduced levels of nitrogen fertilizer

The implications for UK upland sheep systems of reducing nitrogen fertilizer application to perennial ryegrass/white clover swards were studied over 3 years. Sward height (3·5–5·5 cm) was controlled for ewes with lambs until weaning using surplus pasture areas for silage; thereafter, ewes and weaned lambs were grazed on separate areas, and sward height was controlled by adjusting the size of the areas grazed and using surplus pasture areas for silage if necessary. Combinations from three stocking rates [10, 6 and 4 ewes ha−1 on the total area (grazed and ensiled)] and four nitrogen fertilizer levels (150, 100, 50 and 0 kg ha−1) provided six treatments that were replicated three times. Average white clover content was negatively correlated with level of nitrogen fertilizer. The proportion of white clover in the swards increased over the duration of the experiment. Control of sward height and the contribution from white clover resulted in similar levels of lamb liveweight gain on all treatments. All treatments provided adequate winter fodder as silage. It is concluded that the application of nitrogen fertilizer can be reduced or removed from upland sheep pastures without compromising individual animal performance provided that white clover content and sward height are maintained. Resting pastures from grazing by changing ensiled and grazed areas from year to year sustained white clover content over a 3‐year period.

Soil N contributes to the oscillations of the white clover content in mixed swards of perennial ryegrass under conditions that simulate grazing over five years

The effect of the initial N‐supplying capacity of soils (SoilN, 90–230 kg N ha–1 year–1) was tested on the dry‐matter and N yields of pure or mixed white clover and perennial ryegrass swards, managed under simulated grazing over a 5‐year period. The cumulated N harvested in the mixed swards was similar, both for white clover and perennial ryegrass, but the proportion of white clover showed oscillations over a 2‐year period. In the first year, the SoilN effect was similar to that of fertilizer N. During the course of the experiment, the effect was always positive on the pure perennial ryegrass sward, alternately negative and nil for the white clover in the mixed sward and alternately positive and nil for the perennial ryegrass in the mixed sward; the period of these oscillations was 2 years. From the third regrowth period after sowing, the ratio between the actual N concentration and the concentration non‐limiting to growth for the perennial ryegrass in the mixed sward, increased above that of the pure perennial ryegrass sward. It was in turn greater in the soils that were initially poor and then greater in those that were initially rich in soil N. The periodic oscillation of the initial SoilN effects implies that the initial SoilN gradient was alternately compensated and restored. It was concluded that N fluxes are partly responsible for the temporal oscillations in the proportion of white clover in mixed swards.

How does the vertical and horizontal structure of a perennial ryegrass and white clover sward influence grazing?

Mixtures of perennial ryegrass (Lolium perenne L.) and white clover (Trifolium repens L.) sown in alternate rows or in a thoroughly mixed matrix were grazed by sheep, either continuously or during short grazing tests, and were used to investigate the influence of the vertical and horizontal components of the sward structure on defoliation by sheep.In an experiment under continuous grazing, the defoliation intensity was greater for white clover compared with perennial ryegrass leaves (0·80 and 0·58 respectively). In spring, perennial ryegrass leaves were more defoliated than white clover leaves, whereas the reverse was observed in summer. The ratio of the proportion of white clover to perennial ryegrass leaves grazed was negatively correlated with the difference between the surface height of the perennial ryegrass and white clover rows in spring. In both spring and summer, white clover leaves of the same extended leaf length had a higher proportion of them grazed than perennial ryegrass leaves.In another experiment, during short grazing tests with perennial ryegrass–white clover swards that were grazed at the same sward surface height and at the same white clover content as in the previous experiment, there were no significant differences in the proportion of white clover and perennial ryegrass leaves grazed between strips of the two species and thoroughly mixed structures. The proportion of white clover leaves grazed was higher than that of perennial ryegrass leaves.These results show that the differential defoliation by sheep of perennial ryegrass and white clover leaves varies according to their vertical distribution in the mixed canopy, but is little affected by their horizontal distribution. Even small differences in sward surface height between mixed perennial ryegrass and white clover can affect diet selection by sheep to a rather large extent.