Properties Of Ethyl Acetate Extracts Research Articles

Bioprospecting Antibacterial Properties of Fungi Isolated from Kakinada Sea Coast and Eucalyptus Foliage

Background: Fungi produce a wide range of secondary metabolites with bactericidal or bacteriostatic properties. In search of novel antibacterial compounds recently, many fungi of marine and plant origin have been studied for their antimicrobial properties. Aims: This work aimed to study bio-prospect marine and endophytic fungi for their antibacterial properties. objective: To determine the antibacterial properties of ethyl acetate extracts of fungal isolates. Methods: Intensive microbiological methods were followed for isolation, differential growth, and qualitative screening of enzyme production. The isolates were characterised and identified based on morpho-taxonomy, 18S rRNA gene sequencing, and phylogenetic analysis of the tar-get genes. The antimicrobial activity of fungal ethyl acetate extracts against S. aureus, B. sub-tilis, and E. coli was evaluated using a well-diffusion method, and MIC was determined by the microdilution method. Cell lysis was observed through Transmission Electron Microscopy. Results: A BLAST search of 18S rRNA gene sequences of the marine isolates GSBT S13 and GSBT S14 showed 99.3 % sequence similarity with A. glaucus for both isolates and that of endophyte GSBT E3 showed 99.7 % sequence similarity with B. pinkertoniae. Cellulase pro-duction was comparatively higher in GSBT E3 and lipase from GSBT S13 and GSBT S14. Ethyl acetate extracts of GSBT S14 and GSBT E3 showed a clear zone of inhibition by the well-diffusion method, further confirmed by electron microscopy. HR-TEM showed that the ethyl acetate extracts of the isolates appeared to damage the cell membrane, leading to cell shrinkage and death in E. coli and S. aureus. Conclusion: GSBT S13 and GSBT S14 exhibited extracellular amylase, cellulase, and lipase activities. Ethyl acetate extracts of both GSBT S14 and B. pinkertoniae GSBT E3 showed better antibacterial properties against S. aureus.

The Properties of Ethyl Acetate Extract of Frankincense (Styrax benzoin) Using the Maceration Method

This study aims to determine the standardization of ethyl acetate extract of frankincense gum using the maceration method. The method used in this study was to standardize the ethyl acetate extract of frankincense using specific and non-specific parameters. The results of standardization with specific parameters showed that the ethyl acetate extract of frankincense latex was blackish brown in color and had a distinctive odor of frankincense. It contained 86.5% soluble compound in ethanol. The results of standardization with non-specific parameters showed a specific gravity of 0.8213 g/ml and an average moisture content of 1.71%. The chemical components contained are Benzoic acid; Vanillin; transCinnamic acid; Phenol, 2-methoxy-4-propyl-; 2-Propanone, 1-(4) -hydroxy-3-methoxyphenyl)-; Benzoic acid, phenylmethyl ester; (Z)-Cinnamyl benzoate; 2-Propenoic acid, 3-phenyl-,phenylmethyl ester, (E) -; and Cinnamyl cinnamate.

In Vitro Antioxidant and Antimicrobial Potency of Mimosa pudica of Nepalese Terai Region: Insight into L-Mimosine as an Antibacterial Agent.

Aim The study aimed to evaluate the in vitro antioxidant and antimicrobial potency of Mimosa pudica found wildly in the Terai region of Nepal and assess its physicochemical properties, such as total phenolic content (TPC) and total flavonoid content (TFC). Materials and Methods The physicochemical properties of ethyl acetate extract of Mimosa pudica (EAMP), such as extractive value, total ash content, loss on drying, and phytochemical screening, were calculated using standard protocols. The TPC was determined by using the Folin–Ciocalteu method taking gallic acid as standard, and TFC was conducted by using the AlCl3 colorimetric method, using a 96-well plate reader. The in vitro antibacterial activity of different concentrations of the extract against four bacterial ATCC strains was determined by the agar well diffusion method in the Mueller Hinton agar (MHA) medium. The in silico molecular docking model was used to ascertain the antibacterial potency of L-mimosine against the selected strains of bacteria used for the in vitro study by calculating the binding affinity towards the protein of bacteria. Results The preliminary screening of the extract showed the presence of several phytochemicals. The total ash content (7.67%), loss on drying (2.30%), and extractive value (8.966%) were determined by analyzing the crude sample. The total phenolic and flavonoid contents were 418.640 ± 0.018 mg GAE/g (dried extract) and 14.126 ± 0.021 mg QE/g (dried extract), respectively. The extract showed a potent free radical scavenging activity with an IC50 value of 158.95 ± 1.12 µg/mL. The plant extract also demonstrated the antibacterial activity against both Gram-positive bacteria Staphylococcus aureus (15 mm) and Bacillus cereus (22 mm) and Gram-negative bacteria Escherichia coli (17 mm) and Klebsiella pneumoniae (16 mm) at 200 mg/mL concentration of extract. There was a noteworthy binding affinity of antibiotics with almost all selected bacterial proteins with binding energy against Escherichia coli DNA gyrase subunit B (−5.7 kcal/mol), Staphylococcus aureus DNA gyrase subunit B (−6.1 kcal/mol), Bacillus cereus metallothiol transferase (−5.2 kcal/mol), and Klebsiella pneumoniaebeta-lactamase (−6.1 kcal/mole), respectively, with the L-mimosine. Conclusion The findings of the current study suggest that Mimosa pudica from the Terai region of Nepal is rich in phenolic and flavonoid compounds, has a significant impact on bacterial growth inhibition, and has a notable potential to scavenge free radicals (DPPH). According to the in silico analysis, L-mimosine is a potent antibacterial compound that might be utilised to discover novel antibacterial drugs to combat antibiotic resistance.

Антиоксидантные свойства этилацетатного экстракта маклюры оранжевой (Maclura pomifera)

The antioxidant properties of ethyl acetate extracts of fruits, leaves and bark of the Osage orange (Maclura pomifera) tree were studied by a kinetic method using the example of a model reaction of cymene oxidation. The extracts exhibit antioxidant properties. Findings of the research show that fresh orange maclura fruits contain the greatest amount of antioxidants. At a temperature of 348 K, the effective content of antioxidant substances is 1.05 · 10--3 mol/(l · mg). The analysis of the effective content of antioxidants in each extract revealed that it decreases when the extract is stored at room (289 K) temperature. Antioxidant activities of antioxidants were determined --- rate constants of InH + RO•2→ ROOH + In•. According to the content of antioxidants, the ethyl acetate extracts are distributed in the following order: fruits > bark > leaves, and according to antioxidant activity --- bark > fruits > leaves. The dependence of the effective content of antioxidants on the measurement temperature was examines. The study shows that the effective content of antioxidants increases, especially in the extracts of dried fruits, leaves and bark of Osage orange, with decreasing temperature. This is due to a decrease in the additional initiation of primary radicals as a result of the autoxidation of easily oxidizing substances contained in the extracts

Ethyl acetate extract of Terminalia arjuna bark shows antioxidant activity and protects against oxidative stress in rat liver mitochondria

The antioxidant properties of Terminalia arjuna (TA) bark have been identified in this present study. The antioxidant properties of ethyl acetate extract of TA bark were established by DPPH radical scavenging activity, reducing power, H2O2 scavenging activity, scavenging activity of hydroxyl radical, superoxide radical, and nitric oxide radical. This present study also showed the protective effects of ethyl acetate extract against copper-ascorbate-induced oxidative stress in rat liver mitochondria in vitro. The ethyl acetate extract of TA barkaltered the content of reduced glutathione (GSH), levels of lipid-peroxidation (LPO), and protein carbonylation (PCO) (P<0.001) against copper-ascorbate treated rat liver mitochondria, in vitro. Thus, the results indicate that the ethyl acetate extract of TA bark can be considered as a therapeutic agent in the future.

Antiproliferative, Apoptotic, and Antimigration Property of Ethyl Acetate Extract of Calocybe indica against HeLa and CaSki Cell Lines of Cervical Cancer, and its Antioxidant and Mycochemistry Analysis

Background: The incidence rate of cervical cancer is increasing and its existing drugs are becoming more and more resistant. Therefore, we extracted the fruiting body of Calocybe indica edible mushroom in 90% ethyl acetate extract (EAE) and evaluated it as an anticancer property against HeLa and CaSki. Method: We performed cytotoxicity assay by MTT, cell morphological study by phase contrast microscope, and apoptosis study by nuclear morphology via DAPI staining under inverted microscopy; the expressions of proapoptotic and antiapoptotic genes and p53 were examined by Western blotting, cell cycle analysis, and cologenic and cell migration assay. Antioxidant content and activity assays were performed and for mycochemistry analysis of EAE, thin layer chromatography (TLC) was done. Results: EAE-treated HeLa and CaSki cells became round and showed condensed and fragmented nuclei. They inhibited the cell proliferation of both cancer cell lines in a dose-dependent manner. At maximum dose (1250 μg/mL) after 24 h, the cell inhibition percentages of HeLa and CaSki cells were 97.12±10.01 and 98.52±10.08 (p <0.05), respectively. They upregulated the expression of p53, caspase 3, and caspase 9 while down-regulating BcL2 gene. Cell cycle became arrested at G2/M checkpoint of both cancer cell lines by EAE. EAE inhibited colony formation and cell migration. The antioxidant assay showed that EAE contained good amounts of phenolic compounds, flavonoids, and ascorbic acids and had good antioxidant activity. TLC supported the presence of bioactive components. Conclusion: The EAE of C. indica exerts very potent anticervical cancer effects. It is urgent that future studies analyze its bioactive compounds in detail and examine them in animal models.

Phytochemical profile and biological activity of a therapeutic orchid from Anatolia: Dactylorhiza romana subsp. georgica

In Anatolia, the tubers of Dactylorhiza romana subsp. georgica orchid are consumed as food and frequently used in folk medicine to alleviate the symptoms of some disorders. However, these properties have not been scientifically studied until the present day. In this research, we aimed to identify phenolic profile of the orchid tubers and determine their several features and functions. Among these, there are the antioxidant and antimicrobial effects and the inhibitory effect against diabetes related enzymes (α-amylase and α-glucosidase). In this sense, total phenolic content (Folin–Cicalteu assay), total antioxidant capacity (Phosphomolybdate method), antioxidant (ABTS and DPPH assays) and enzyme inhibition effects of tuber extracts obtained with different solvents (chloroform, ethyl acetate, methanol, ethanol, or hexane) were determined by using spectrophotometric methods, and the phenolic profile was also analysed by HPLC. In addition, antimicrobial activity of the extracts against 3 g (+) and 3 g (−) bacteria and 2 forms of yeasts was determined by disc diffusion and micro dilution methods. Based on our findings, the total phenolic content and antioxidant properties of ethyl acetate extract were considerably higher than the others. The most abundant among the screened phenolic standards was found to be benzoic acid. The highest α-amylase and α-glucosidase enzyme inhibition was measured in methanol and chloroform extracts, respectively. The highest antibacterial (B. cereus) and anticandidal (C. tropicalis and C. albicans) activities were detected in the chloroform and ethyl acetate extracts, respectively. In short, it was clearly seen that the D. romana subsp. georgica tuber had a great potential for being used in food and drug industries as a source of bioactive compounds.

Antibacterial, anti-inflammatory and peroxidase-mediated cyclooxygenase-1 inhibitory properties of Fusarium solani extract

Context: Nigerian soil fungi population is unexplored. It is hypothesized that they harbour new bioactive chemicals. This hypothesis is based on the large percentage of currently approved medicines that originated from soil-inhabiting micro-organismsObjectives: To investigate the antimicrobial and anti-inflammatory properties of Fusarium solani ethyl acetate (EtOAc) extract selected based on its broad spectrum of antimicrobial potential in an overlay experiment with seven other soil fungi strains.Materials and methods: Fungus number 6 (F6), identified by molecular characterization as Fusarium solani (Mart.) Sacc (Nectriaceae) was selected for studies from eight purified soil fungi due to its superior broad-spectrum antibiotics producing potential following agar overlay experiment. F6 was fermented for 21 d and the minimum inhibitory concentration (MIC) of its EtOAc fermentation extract (dose range: 12.5–100 µg/mL) was determined using agar dilution method for Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Escherichia coli, Salmonella typhi and anti-inflammatory properties determined using rat-paw (250–500 mg/kg) and xylene induced oedema (250–500 µg/kg) (in Swiss albino rats and mice) models, respectively. The ability of the extract to inhibit cyclooxygenase (COX) enzyme was also determined in vitro using Cayman test kit-760111.Result: The MIC of the EtOAc extract was <12.5 µg/mL for S. aureus, P. aeruginosa and Escherichia coli. It inhibited xylene induced oedema by 65% compared with 61% observed for diclofenac and was significantly (p < 0.05) better than diclofenac in rat-paw-oedema model within the first phase of inflammation. The extract inhibited COX-1 peroxidase-mediated activities with an IC50 below 5 µg/mL.Conclusions: The extract exhibited strong antibacterial and anti-inflammatory properties, warranting further investigations into therapeutic potential of this fungus. This study design can be adapted in soil fungi metabolomic investigations. We report for the first time the potent anti-inflammatory property of the ethyl acetate extract of soil strain of F. solani with a possible mechanism of action that involves the inhibition of COX enzyme.

Chemical constituents, in vitro antioxidant and antimicrobial properties of ethyl acetate extract obtained from Cytisus triflorus l’Her

The present study investigates the phenolic composition, antioxidant and antimicrobial activities of an ethyl acetate extract from C. triflorus L’Her. The phytochemical study on the aerial parts of C. triflorus belonging to the Fabaceae family led to the isolation and structural elucidation of 5-Hydroxy-7-O-glucosylflavone (P1), 5-Hydroxy-7-O-galactosylflavone (P2), 5,7-Dihydroxy-flavone (P3), 5,7,3’-Trihydroxy,4’-methoxy-flavone (P4). These compounds were identified by 1D and 2D NMR combined analysis as well as by UV.Ethyl acetate extract of C. triflorus showed a significant and dose-dependent antioxidant activity in vitro, related to the presence of phenolics (180.33 ± 12.22 µg GAE/mg) and flavonoids (16.78 ± 1.54 µg QE/mg). The antimicrobial activity was evaluated in vitro against Staphylococcus aureus CECT 240 and Escherichia coli CECT 4099, by agar-diffusion method. The most active antibacterial activity was expressed by ethyl acetate extract of C. triflorus against Gram-positive bacteria S. aureus. The gathered results suggest that C. triflorus polyphenols and flavonoids are closely associated to its antioxidant and antimicrobial properties.

EVALUATION OF ANTIOXIDANT, ANTIBACTERIAL, AND GAS CHROMATOGRAPHY-MASS SPECTROMETRY ANALYSIS OF ETHYL ACETATE EXTRACT OF STREPTOMYCES OMIYAENSIS SCH2

Objective: The aim of this study was to evaluate the antibacterial and antioxidant properties of ethyl acetate extract of Streptomyces omiyaensis SCH2. The chemical constituents of the extract were investigated using gas chromatography-mass spectrometry (GC-MS).Methods: Secondary metabolites from S. omiyaensis were produced by submerge fermentation using ISP2 medium with 3% NaCl (w/v) for 21 days. The culture filtrate was extracted with ethyl acetate. The chemical constituents were detected in the GC-MS analysis. Antibacterial activity was performed using disc diffusion and broth microdilution methods. Antioxidant activity was evaluated by determining the reducing power capacity and free radical scavenging assays.Results: The GC-MS analysis of the SCH2 extract revealed the presence of four compounds. The main constituents were 2-phenylacetamide (79%). The extract exhibited the highest zone of inhibition against some pathogenic bacteria such as Enterobacter cloacae, Klebsiella pneumoniae, and Bacillus subtilis. In addition, the lowest minimum inhibitory concentration (IC) and minimum bactericidal concentration values of extract were obtained for E. cloacae (0.125 and 4 mg/ml). The extract showed antioxidant potential with IC50 values of 2.078.13±24.58 μg/ml and 475.74±4.56 μg/ml for 2,2-diphenyl-1-picryl-hydrazyl and 2,2′-azino-bis[3-ethylbenzothiazoline-6-sulphonic acid] assays, respectively. The ferric reducing antioxidant power value of extract was 110.36±1.75 mmol FeSO4.7H2O/g extract.Conclusion: This study indicated that S. omiyaensis extract possesses antibacterial and antioxidant activities. GC-MS analysis revealed the presence of major chemical constituents, acetamide, and pyrrolopyrazine which could be responsible for the biological activities. S. omiyaensis extract could be used as a potential of natural antibacterial and antioxidant agents for pharmaceutical and medical applications.

Antibacterial Activity of Ethyl Acetate Extracts from Algerian Cupressus sempervirens var Against Some Human Pathogens Bacteria

Cupressus sempervirens var. is a plant rich in bioactive compounds and it has been a typical used in folk medicine for thousands of years. In Algeria, Cupressus sempervirens is widely used as traditional remedies for treatment of different complicate infectious diseases this study was conducted to evaluate antibacterial properties of ethyl acetate extracts of Cupressus sempervirens against gram negative and gram positive. The plant was collected from Chlef, in Tenes is a region of Algeria Mediterranean, then, the plant extract was prepared by maceration .The antibacterial activity of the extracts was investigated against 6 clinical and reference strains based on two assays: the well diffusion method and the agar dilution method. Cuperssus sempervirence acted as the agent antibacterial by having the highest (MIC/MBC 0.25v/v) against B.subtilis ATCC 6133, E.coli followed by Coagulase-Negative Staphylococci, Microccocus lutea (MIC/MBC 0.5v/v). Results of the phytochemical studies of ethyl acetate extract revealed the presence low amount of total phenolic according to the spectrophotometric method. Cupressus sempervirens has broad spectrum antibacterial activity and can be consider as a potential source of new classes of antibiotics that could be useful for infectious disease chemotherapy and control.

A Comparative Study of the Phytochemical, Antioxidant and Anti-Inflammatory Properties of Ethyl Acetate Extract of Asphodelus Tenuifolius Cav Recovered by Different Extraction Techniques

A Comparative Study of the Phytochemical, Antioxidant and Anti-Inflammatory Properties of Ethyl Acetate Extract of Asphodelus Tenuifolius Cav Recovered by Different Extraction Techniques

In vitro α-glucosidase inhibition, antioxidant, anticancer, and antimycobacterial properties of ethyl acetate extract of Aegle tamilnadensis Abdul Kader (Rutaceae) leaf.

The present study was aimed to investigate in vitro α-glucosidase inhibition, antioxidant, anticancer, and antimycobacterial activities of the ethyl acetate extract of A. tamilnadensis leaves. The extract recorded strong α-glucosidase inhibition with an IC50 value of 100 μg/ml. The antioxidant potential of the extract was evaluated by nitric oxide radical inhibition, lipid peroxidation inhibition, ferric thiocyanate, and ABTS radical scavenging assay, and the extract recorded significant antioxidant activity. The ferric thiocyanate activity of extract was superior to butylated hydroxyl anisol (BHA), the standard antioxidant agent. The anticancer activity of the extract was evaluated against (1) breast cancer cell lines (MDAM B-231), (2) cervical cancer cell lines (HeLa), and (3) lung cancer cell line (A 549) using MTT assay, and significant activity was recorded against A 549 with an IC50 value of 64 μg/ml. Further studies on the morphology, acridine orange/ethidium bromide staining, and cell cycle analysis by flow cytometry confirm the extract-induced apoptosis in A 549. This extract also recorded significant anti-tuberculosis activity against Mycobacterium smegmatis. The current study suggests that the ethyl acetate extract of A. tamilnadensis is a potential source of natural α-glucosidase inhibitor and antioxidant for protection as well as prevention of life-threatening diseases like cancer.

A flavonoid isolated from Streptomyces sp. (ERINLG-4) induces apoptosis in human lung cancer A549 cells through p53 and cytochrome c release caspase dependant pathway

The aim of this study was to investigate the anticancer activity of a flavonoid type of compound isolated from soil derived filamentous bacterium Streptomyces sp. (ERINLG-4) and to explore the molecular mechanisms of action. Cytotoxic properties of ethyl acetate extract was carried out against A549 lung cancer cell line using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Cytotoxic properties of isolated compound were investigated in A549 lung cancer cell line, COLO320DM cancer cell line and Vero cells. The compound showed potent cytotoxic properties against A549 lung cancer cell line and moderate cytotoxic properties against COLO320DM cancer cell line. Isolated compound showed no toxicity up to 2000μg/mL in Vero cells. So we have chosen the A549 lung cancer cell line for further anticancer studies. Intracellular visualization was done by using a laser scanning confocal microscope. Apoptosis was measured using DNA fragmentation technique. Treatment of the A549 cancer cells with isolated compound significantly reduced cell proliferation, increased formation of fragmented DNA and apoptotic body. Activation of caspase-9 and caspase-3 indicated that compound may be inducing intrinsic and extrinsic apoptosis pathways. Bcl-2, p53, pro-caspases, caspase-3, caspase-9 and cytochrome c release were detected by western blotting analysis after compound treatment (123 and 164μM). The activities of pro-caspases-3, caspase-9 cleaved to caspase-3 and caspase-9 gradually increased after the addition of isolated compound. But Bcl-2 protein was down regulated after treatment with isolated compound. Molecular docking studies showed that the compound bound stably to the active sites of caspase-3 and caspase-9. These results strongly suggest that the isolated compound induces apoptosis in A549 cancer cells via caspase activation through cytochrome c release from mitochondria. The present results might provide helpful suggestions for the design of antitumor drugs toward lung cancer treatment.

Annona muricata leaves induce G1 cell cycle arrest and apoptosis through mitochondria-mediated pathway in human HCT-116 and HT-29 colon cancer cells

Ethnopharmacological relevanceAnnona muricata known as “the cancer killer” has been widely used in the traditional medicine for the treatment of cancer and tumors. The purpose of this study is to investigate the anticancer properties of ethyl acetate extract of Annona muricata leaves (EEAM) on HT-29 and HCT-116 colon cancer cells and the underlying mechanisms. Materials and methodsThe effect of EEAM on the cell proliferation of HT-29 and HCT-116 cells was analyzed by the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium) assay. High content screening system (HCS) was applied to investigate the cell membrane permeability, mitochondrial membrane potential (MMP), nuclear condensation and cytochrome c translocation from mitochondria to cytosol. Reactive oxygen species (ROS) formation, lactate dehydrogenase (LDH) release and activation of caspase-3/7, -8 and -9 were measured while treatment. Flow cytometric analysis was used to determine the cell cycle distribution and phosphatidylserine externalization. The protein expression of Bax and Bcl-2 was determined using immunofluorescence analysis. In addition, the potential of EEAM to suppress the migration and invasion of colon cancer cells was also examined. ResultsEEAM exerted significant cytotoxic effects on HCT-116 and HT-29 cells as determined by MTT and LDH assays. After 24h treatment, EEAM exhibited the IC50 value of 11.43±1.87µg/ml and 8.98±1.24µg/ml against HT-29 and HCT-116 cells, respectively. Flow cytometric analysis demonstrated the cell cycle arrest at G1 phase and phosphatidylserine externalization confirming the induction of apoptosis. EEAM treatment caused excessive accumulation of ROS followed by disruption of MMP, cytochrome c leakage and activation of the initiator and executioner caspases in both colon cancer cells. Immunofluorescence analysis depicted the up-regulation of Bax and down-regulation of Bcl-2 proteins while treated with EEAM. Furthermore, EEAM conspicuously blocked the migration and invasion of HT-29 and HCT-116 cells. ConclusionsThese findings provide a scientific basis for the use of A. muricata leaves in the treatment of cancer, although further in vivo studies are still required.

Evaluation of Phenolic Content and Antioxidant Property of &amp;lt;i&amp;gt;Crossandra infundibuliformis&amp;lt;/i&amp;gt; Leaves Extracts

The present study was aimed to investigate the phenolic content and antioxidant property of Crossandra infundibuliformis leaves. Shade dried leaves were successively extracted and determined phenolic and flavonol content of the extracts. Six different in vitro antioxidant assays such as 1, 1’-diphenyl-2-picrylhydrazyl (DPPH), lipid peroxidation (LPO), nitric oxide, hydrogen peroxide radical scavenging, reducing power of the extracts and total antioxidant capacity (values were expressed as mg ascorbic acid (vitamin C) equivalent antioxidant capacity per gram of extracts) assays were followed to determine the antioxidant capacity of the extracts. The results of this study indicated that methanol extract possess higher level of phenolic (98.52 mg of gallic acid/g of extract) and flavonol (84.59 mg of rutin/g of extract) constituents. The antioxidant property of ethyl acetate extract was significantly higher than other extracts in DPPH method. Moreover, methanol extract has significantly higher antioxidant activity in LPO inhibition (IC50 value of 70.66 μg/ml), hydrogen peroxide (IC50 value 130.33 μg/ml) and reducing capacity of the extract (1.19) than other chloroform and ethyl acetate extracts. The strong correlation was observed for phenolic content and antioxidant activities of the extracts ensuring the involvement of phenols in antioxidant activity. However, results suggests that Crossandra infundibuliformis leaves possess antioxidant activity.

Eco-friendly approach using marine actinobacteria and its compounds to control ticks and mosquitoes

Ticks and mosquitoes are ectoparasitic arthropods that can transmit a variety of diseases to humans and animals during blood feeding and causing serious infectious disorders. The purpose of the present study was to assess the acaricidal and insecticidal property of ethyl acetate extract and its compounds isolated from marine actinobacteria, Streptomyces VITSTK7 sp. against the larvae of cattle ticks, Haemaphysalis bispinosa and Rhipicephalus (Boophilus) microplus (Acari: Ixodidae); fourth-instar larvae of malaria vector, Anopheles subpictus; and filarial vector, Culex quinquefasciatus (Diptera: Culicidae). The ethyl acetate extract was loaded on silica gel column and separated with chloroform, methanol, and acetone as the solvents system. The separation of fractions was visualized by the thin layer chromatography (TLC) plate, further confirmed by high-performance liquid chromatography, and followed by gas liquid chromatography. Three major fractions were analyzed in mass spectroscopy (MS) and matched with existing compounds in the data base. Based on the fragment pattern, it led to the major compounds which were predicted as cyclopentanepropanoic acid, 3,5-bis(acetyloxy)-2-[3-(methoxyimino)octyl], methyl ester (13.3 %) 1; 5-azidomethyl-3-(2-ethoxy carbonyl-ethyl)-4-ethoxycarbonylmethyl-1H-pyrrole-2-carboxylic acid, ethyl ester (18.2 %) 2; and akuammilan-16-carboxylic acid, 17-(acetyloxy)-10-methoxy, methyl ester (16R) (53.3 %) 3. The maximum efficacy was observed in compounds 1, 2, and 3, and the ethyl acetate extract of Streptomyces VITSTK7 sp. against the larvae of H. bispinosa (LC(50) = 1,573.36, 1,333.09, 1,073.29, and 409.71 ppm; r(2) = 0.0.990, 0.934, 0.935, and 0.908), R. microplus (LC(50) = 1,877.86, 815.83, 1,631.14, and 441.54 ppm; r(2) = 0.981, 0.926, 0.0970, and 0.915), A. subpictus (LC(50) = 273.89, 687.69, 464.75, and 223.83 ppm; r(2) = 0.758, 0.924, 0.841, and 0.902), and C. quinquefasciatus (LC(50) = 430.06, 881.59, 777.0, and 195.70 ppm; r(2) = 0.839, 0.859, 0.870, and 0.882), respectively. Results of the present study provide evidence that the maximum parasitic activity of ethyl acetate extract and a synergistic effect of combinations of different compounds have been suggested. The control (distilled water) showed nil mortality in the concurrent assay. In the present study, a novel, targeted, simple, and eco-friendly approach has been suggested to control blood-feeding parasites.

Bioactive anthraquinones from endophytic fungus Aspergillus versicolor isolated from red sea algae

The marine fungus Aspergillus versicolor was isolated from the inner tissue of the Red Sea green alga Halimeda opuntia. The fungus was identified by its morphology and 18s rDNA. Cultivation of this fungal strain led to a new metabolite named isorhodoptilometrin-1-methyl ether (1) along with the known compounds emodin (2), 1-methyl emodin (3), evariquinone (4), 7-hydroxyemodin 6,8-methyl ether (5), siderin (6), arugosin C (7), and variculanol (8). The structures were elucidated on the basis of NMR spectroscopic analysis and mass spectrometry. The biological properties of ethyl acetate extract and compounds 1-3 and 6-8 were explored for antimicrobial activity, anti-cancer activity and inhibition of Hepatitis C virus (HCV) protease.

Anti-proliferative properties of ethyl acetate extract of Phellinus linteus grown on ginseng (Panax ginseng) on HT-29 human colon carcinoma cells through inducing apoptosis

Phellinus linteus (PL) and ginseng (Panax ginseng, PG) have been widely used as traditional herbal medicines in East Asia. In this study, we evaluated whether the extract of P. linteus grown on ginseng (PGP) exhibited an more enhanced anti-proliferative activity on HT-29 human colon carcinoma cells than PL or PG extract. PGP EtOAc extract inhibited HT-29 cancer cell viability stronger than PG or PL EtOAc extract. FACS analysis revealed that PGP EtOAc extract increased sub-G1 (apoptotic) HT-29 cell population. The nuclear structures of HT-29 cells were disrupted, resulting in apoptotic fragments (45 kDa) of lamin B after treating PGP EtOAc extract. PGP EtOAc extract induced activation of caspase-8 and -9 in HT-29 cells. These results suggest that the PGP EtOAc extract has a direct anti-cancer effect through apoptosis and cell cycle blockade in HT-29 human colon carcinoma cells.

Antihyperglycaemic Properties of the Ethyl acetate Extract of Dennettia tripetala in Diabetic Rats

Antihyperglycemic properties of chloroform (CDT), ethyl acetate (EDT) and methanolic (MDT) extracts of D. tripetala were investigated using alloxan- and streptozotocin-induced hyperglycemic rats. An oral glucose tolerance test (OGTT) was performed in EDT-pretreated in both normal and alloxan-induced hyperglycemic rats. Tolbutamide (TLB) was used as the reference drug. TLB (100 mg/g), EDT (100 mg/kg), MDT (100 mg/kg) significantly (p&lt;0.05) decreased the plasma glucose level at different time intervals after the administration of the extracts/drug. The antihyperglycemic activity of TLB and EDT started at 2 and 3h, respectively, and lasted till 10h. EDT caused the highest reduction (46%) in plasma glucose level in 10h after treatment. In OGTT, EDT (50 mg/kg) significantly (p&lt;0.05) increased glucose tolerance in both normal and hyperglycemic rats. This activity was more prominent in the alloxan-induced hyperglycemic rats. TLB (100 mg/kg) initially caused a slight reduction in plasma glucose level followed by steady level throughout the period of the study. Furthermore, EDT (30 mg/kg) significantly (P&lt;0.05) decreased the plasma glucose level at 6, 8 and 10 h post treatment, when compared with TLB (100 mg/kg). In conclusion, EDT showed promising antihyperglycemic activity, which justified the use of the plant extract by the natives in managing diabetes mellitus.