Properties In Traditional Medicine Research Articles

Protective Effect of Deer Heart Peptide on Cardiac Injury in Mice

Protective Effect of Deer Heart Peptide on Cardiac Injury in Mice

Silver nanoparticles green-formulated by Scrophulariastriata: A novel chemotherapeutic drug for the treatment of nerve cancer

The plants use because of their compatibility with the abundance and environment are usually prioritized in synthesizing the silver nanoparticles. In addition, because of their abundance and need lack for special conditions and growth nutrients, plants are suitable options for the production of silver nanoparticles using biological methods. One of these plants is Scrophularia striata. It belongs to the Scrophulariacae family family and has many medicinal properties in traditional medicine. It has been traditionally consumed by local people recently for helping to heal wounds and disinfecting them, however, there have not been many studies on it. We herein demonstrate a novel Ag NPs green mediated by S. striata aqueous extract for the nerve cancer treatment under in vitro conditions. Physicochemical and structural properties of the nanoparticles biomaterial were determined by UV-Vis spectrum, FT-IR, and FE-SEM. The IC50 of Ag NPs against DPPH was 114 μg/mL. In the oncological part of this research, the statue of normal and human peripheral nerve sheath tumor (BL1391 and S462) cells was determined. The IC50 of Ag NPs was 191 and 172 μg/mL against S462 and BL1391. It seems that the prepared nanoparticles have stopped the nerve cancer cells growth and the recent cancer cells have removed with high concentration of nanoparticles. According to the results of this research, it is suggested that the synthesized Ag NPs may be used as a suitable therapeutic agent against several nervous system tumors.

Sour Jujube (Ziziphus jujuba var. spinosa): A Bibliometric Review of Its Bioactive Profile, Health Benefits and Trends in Food and Medicine Applications.

Research on the comprehensive utilization of sour jujube and its beneficial properties to human health has attracted extensive attention. This study aims to conduct a bibliometric analysis of the bioactive profile of sour jujube and future trends in applications. The research advancements within this field from 2000 to 2023 were addressed using the Web of Science database and VOSviewer. Among the 322 results, the most frequent keywords of bioactivity are flavonoids, antioxidants, saponins, insomnia, polyphenols, terpenoids and anti-inflammatory; the most studied parts of sour jujube are seeds, fruits and leaves; the published articles with high citations mainly focus on identification, biological effects and different parts distribution of bioactive compounds. The bioactivity of various parts of sour jujube was reviewed considering their application potential. The seeds, rich in flavonoids, saponins and alkaloids, exhibit strong effects on central nervous system diseases and have been well-developed in pharmacology, healthcare products and functional foods. The pulp has antioxidant properties and is used to develop added-value foods (e.g., juice, vinegar, wine). The leaves can be used to make tea and flowers are good sources of honey; their extracts are rich sources of flavonoids and saponins, which show promising medicinal effects. The branches, roots and bark have healing properties in traditional folk medicine. Overall, this study provides a reference for future applications of sour jujube in food and medicine fields.

Spinacia Oleracea: Exploring the Therapeutic Potential in Persian Medicine and Modern Pharmacology.

Spinach is a widely cultivated dark leafy vegetable highly regarded for its medicinal properties in traditional Persian medicine. It is rich in vitamins, minerals, flavonoids, carotenoids, and other bioactive compounds, and this review aims to explore the historical applications of spinach in Persian medicine and juxtapose them with current scientific evidence. Despite its historical significance, there remains a need to comprehensively evaluate and integrate traditional knowledge with modern research on the therapeutic benefits of spinach. To achieve this, a comprehensive search was conducted in Persian medicine references and scientific databases to gather information on the traditional uses, chemical composition, and pharmacological effects of spinach. Studies that met the inclusion criteria were meticulously categorized, and relevant data were analyzed to draw insightful comparisons. Persian medicine describes spinach as a nutrient-rich, laxative, and fast-digesting agent with therapeutic effects on inflammation, lung diseases, back pain, sore throats, jaundice, urinary disorders, joint pain, eye inflammation, insomnia, dementia, and more. Modern studies have substantially corroborated these traditional uses, revealing that spinach possesses antioxidant, anti-inflammatory, anti-cancer, blood sugar-lowering, lipid-lowering, anti-obesity, neurological, ocular, and musculoskeletal effects. Spinach exhibits a wide range of beneficial effects on various health conditions. Its widespread availability, low cost, and exceptional nutritional richness position it as a promising candidate for further investigation. Future studies should explore the clinical effectiveness of spinach in various diseases, while taking into consideration the principles emphasized in Persian medicine to guide research and inform therapeutic strategies.

An Insight into the Traditional Uses, Phytochemicals, and Pharmacological Properties of Polyalthia bullata King

Abstract: Polyalthia bullata King or Tongkat Ali Hitam, a term in the Malay language, encompasses a diverse group of plants belonging to different genera of the Annonaceae family. The native range of this species is Myanmar to Peninsula Malaysia, Borneo. It is a shrub or tree and grows primarily in the wet tropical biome. These plants have been utilized in Malaysia and Southeast Asia as an herbal aphrodisiac. The extracts of this plant have been reported to exert pharmacological activities, such as antioxidant, cytotoxicity, and aphrodisiac properties. The therapeutic properties of P. bullata are attributed to the presence of alkaloids and flavonoids. This study thoroughly analyzes the phytochemical and pharmacological research on P. bullata and its traditional medicinal properties. The review aims to provide a critical update on the scientific knowledge of P. bullata and to identify novel possibilities for investigating its therapeutic potential. There is limited scientific research on the phytochemical components and pharmaceutical application of P. bullata. To date, only a few compounds have been identified and isolated. There is a need for future studies to focus on the extraction technology, phytochemical profiling, and isolation of bioactive compounds from P. bullata to fill the research gap as well as to provide an important theoretical basis and valuable references for researchers and pave the way for the discovery and development of new and existing drugs.

Potent antibacterial activity of Terminalia chebula-based herbal soap formulation against propionibacterium acnes and its cytotoxic evaluation on human skin fibroblast cells

Background: Recently, many approaches have been conducted in natural skin healthcare, with herbal remedies gaining prominence due to their perceived efficacy and fewer side effects. Herbal soaps, in particular, are noted for their antimicrobial, anti-inflammatory, and antioxidant properties, making them appealing for acne-causing bacteria such as Propionibacterium acnes, Terminalia chebula, a medicinal plant with a rich history of traditional medicinal properties, including antibacterial effects. However, limited information exists on its activity against acne-causing bacteria and its cytotoxicity on human skin cells. Methods: Our study demonstrated the anti-propionibacterium activity using a novel Terminalia chebula herbal soap formulation (TC-HSF), and to determine its cytotoxicity on Human skin fibroblast cells, Terminalia chebula aqueous extract was used to make herbal soap, the antibacterial activity was performed against wild isolated propionibacterium acne strain using well-diffusion method, MIC and time-kill Kinetics, several concentrations were evaluated for cytotoxicity on Human skin fibroblast cell lines. Results Our findings indicate a potent activity of TC-HSF against P.acne at 0.18mg\ml with lower CFU\ml in tested intervals and high cell viability 98%, IC50 Value was 50mg\ml. Conclusion: Our study highlights the promising antibacterial efficacy of TC-HSF against Propionibacterium acnes and its cytotoxic effects on human skin fibroblast cells. The research adds valuable insights into the potential of TC-HSF for acne management and underscores the importance of dosage considerations in the formulation of herbal soap products.

Enzyme-treated red rice (Oryza sativa L.) bran extracts mitigate inflammatory markers in RAW 264.7 macrophage cells and exhibit anti-inflammatory efficacy greater/comparable to ferulic acid, catechin, γ-tocopherol, and γ-oryzanol

Ethnopharmacological relevanceRice (Oryza sativa L.), a staple food for a significant portion of the global population, has been recognized for its traditional medicinal properties for centuries. Rice bran, a by-product of rice milling, contains many bioactive compounds with potential pharmaceutical and therapeutic benefits. In recent years, research has highlighted the anti-inflammatory potential of rice bran, contributed by the bioactive components concentrated in their bran but, unfortunately, entrapped in the bran matrix, with limited bioavailability. Previous studies have reported that the enzymatic treatment of rice bran improves the bran's bioactive compound profile but did not investigate its impact on chronic conditions such as inflammation. Aim of the studyThis study investigates the anti-inflammatory effects of endo-1,4-β-xylanase (ERB) and Viscozyme (VRB) treated red rice bran extracts against lipopolysaccharide-induced inflammation in RAW264.7 macrophages in comparison with non-enzyme-treated bran (CRB). Further established their efficacy with known anti-inflammatory compounds-ferulic acid (FA), catechin (CAT), γ-tocopherol (GTP), and γ-oryzanol (ORZ). Materials and methodsThe RAW 264.7 macrophage cells were pre-treated with non-toxic concentrations (10–200 μg/mL) of FA, CAT, GTP, ORZ, CRB, ERB, and VRB, followed by inflammatory stimulation with LPS for 24 h. Further, the cell supernatant and pellets were harvested to study the anti-inflammatory effects by evaluating and measuring their efficacy in inhibiting pro-inflammatory cytokines (TNF-α, IL-6, IL-10, IL-1β) and mediators (ROS, NO, PGE2, COX2, iNOS) through biochemical, ELISA, and mRNA expression studies. ResultsThe findings showed that both ERB and VRB effectively inhibited the production of pro-inflammatory markers (TNF-α, IL-6) and mediators (ROS, NO, PGE2) by downregulating mRNA expressions of inflammatory genes (TNF-α, IL-1β, IL-6, IL-10, COX2, iNOS) and demonstrated anti-inflammatory efficacy higher than CRB. On comparison, ERB demonstrated exceptional efficacy by causing a reduction of 48% in ROS, 20% in TNF-α, and 23% in PGE2 at 10 μg/mL, surpassing the anti-inflammatory capabilities of all the bioactive compounds, FA and ORZ, respectively. At the same time, VRB exhibited remarkable efficacy by reducing NO production by 52% at 200 μg/mL and IL-6 by 66% at 10 μg/mL, surpassing FA, CAT, ORZ, and GTP. Further, ERB downregulated the mRNA expression of IL-10 and iNOS, while VRB downregulated TNF-α, IL-1β, and COX2 expression. Both extracts equally downregulated IL-6 expression at 10 μg/mL, demonstrating the efficacy more remarkable/on par with established anti-inflammatory compounds. ConclusionsOverall, enzyme-treated rice bran/extract, particularly ERB, possesses excellent anti-inflammatory properties, making them promising agents for alternatives to contemporary nutraceuticals/functional food against inflammatory diseases.

Treatment of denture stomatitis using iron nanoparticles green-synthesized by Silybum marianum extract

Abstract Stomatitis or generalized inflammation of the mouth includes inflammation or pain in the mouth. Natural compounds are one of the best options for stomatitis treatment. Silybum marianum has many medicinal properties in traditional medicine. In recent research, iron nanoparticles were formulated by S. marianum. The research aim was to determine the Fe nanoparticles’ (FeNPs) efficacy in denture stomatitis treatment. FeNPs were characterized by TEM, FE-SEM, and UV-Visible. This was a clinical trial study with 60 patients who received FeNPs. The patients in 14 days were suggested to use mouthwash 1 time per 6 h each time 15–20 drops for 120–180 s and after that they should avoid drinking and eating for 0.5 h; patients in the two groups were offered to apply the drug. At each visit, mycological samples were taken for culture from the palatal mucosa. Inflammation amount and erythema were determined in each session and determined with a graded blade and recorded. The erythema surface of the palatal was significantly decreased in the group at follow-up visits compared to the pretreatment condition.

Compilation Of Ethnomedicinal Knowledge Of Acanthaceae Species By Ethnic Group In Sabah And Sarawak, Malaysia

With over 4 000 species worldwide, the Acanthaceae family is a large and diverse group of plants. Within the regions of Sabah and Sarawak, over a hundred species of Acanthaceae have been identified, with many of them being employed for their traditional medicinal properties. The ethnomedicinal knowledge of Acanthaceae by ethnic in Sabah and Sarawak is a complex and fascinating topic. Different ethnic groups in the region have different uses for this plant and their knowledge is often passed down through generations. Hence, the aim for this paper is to compile and comprehend all published information on the usage of Acanthaceae species by the ethnic group in Sabah and Sarawak for traditional medicinal purposes. From our data collections, seven species of Acanthaceae is known being used traditionally to treat ailments such as stomach aches, skin problems or headaches either by boil the leaves and drink as a tea or applied as a paste. For example, the Murut people of Sabah use the leaves of Graptophyllum pictum (L.) Griff. to treat headache while the Dayak people of Sarawak also use the leaves of G. pictum for bathing to treat jaundice for baby. This knowledge is an invaluable asset that merits preservation and can facilitate the development of novel disease treatments. Data from this paper also important for the conservation of Acanthaceae plants as well as for the development of new medicinal and food products. To date, the knowledge of these plants is still being actively researched and documented.

Survey on Medicinal Plants and Herbs in Traditional Iranian Medicine with Anti-oxidant, Anti-viral, Anti-microbial, and Anti-inflammation Properties

Abstract: The review aims to summarize the major and dominant natural antioxidants and their resources from medicinal and herbal plants with antiviral, anti-inflammation and antimicrobial activities. For this review manuscript, online databases, including Web of Science, Scopus, PubMed, and Science Direct, were searched for papers published from 1960 to November, 2021. Search terms consisted of “medicinal plants”, “traditional Iranian medicine”, “traditional Persian medicine”, “pharmaceutical properties”, “antioxidant activity”, “antiviral activity”, “anti-inflammation”, “antimicrobial” and “antibacterial activities”. (-)-Epicatechin, Caffeic acid, Gallic acid, Hydroxytyrosol, Kaempferol, and Resveratrol are some of the most important chemical compounds with antioxidant properties. Rosmarinic acid, Caffeic acid, Carnosol, P-Coumaric acid, Carnosic acid, Luteolin, Apigenin, and Kaempferol are the major chemical compounds with antiviral properties. Curcumin, Colchicine, Resveratrol, Capsaicin, (-)-Epigallocatechin, Quercetin, Myristicin, and Elmicin are the principal chemical compounds with anti-inflammatory properties. Isoeugenol, Coumarin, Piperonal, Scoparone, Spathulenol, D-Limonene, and Myrcene are the principal chemical compounds with antibacterial properties in traditional Iranian medicine. Persian traditional medicine, or Iranian traditional medicine, is one of the main ancient forms of traditional medicine, which has influenced knowledge regarding other medicinal plants in various countries. It has also been considered one of the most well-known traditional and holistic systems of medicine.

Gastroprotective potential of red onion (Allium cepa L.) peel in ethanol-induced gastric injury in rats: Involvement of Nrf2/HO-1 and HMGB-1/NF-κB trajectories

Ethnopharmacological relevanceThe utilization of plants with therapeutic properties in traditional medicine has a longstanding practice. Among them, the well-known Allium cepa L. commonly known as onion has been valued for its anti-inflammatory and antioxidant potential in the treatment of various ailments, including gastric ulcers. Aim of the studyThis study investigated the gastroprotective potential of red onion peel extract and its fractions in a rat model of ethanol-induced gastric ulcer. Moreover, their phytochemical profiles were compared to identify the active metabolites. Materials and methodsMass spectrometry-based metabolomics and chemometrics were performed for phytochemical analysis. Ethanol-induced gastric ulcer model was used to assess the gastroprotective activity. Nine groups of rats were allocated as follows: Group 1 was the normal control; Group 2 rats were used as a positive control/model and received 1 mL of absolute ethanol; and Group 3 rats were treated with famotidine at a dose of 20 mg/kg orally. Group 4 and 5 rats were treated with total acidified ethanolic extract (T1, T2). Group 6 and 7 rats were treated with anthocyanins-rich fractions (P1, P2). Groups 8 and 9 were the flavonoids-rich fraction (S1, S2) treatment. Prior to scarification, the ulcer index in mm was obtained from gastric tissues photographed beside a ruler with further analysis using ImageJ software. ResultsSeventy key major and discriminatory metabolites were identified including flavonoids, anthocyanins, phenolic acids, and miscellaneous compounds. The examined extract and its fractions significantly reduced the ulcer index and inflammatory cytokines via downregulating HMGB-1/NF-κB. Also, they augmented the expression of Nrf2/HO-1 and reduced NOX1/4 mRNA expression. Moreover, there was a significant reduction in the oxidative stress and apoptotic biomarkers as well as a noticeable enhancement in histopathological changes of the stomach tissues. ConclusionRed onion peels have a promising dose dependent gastroprotective potential in alcohol-induced ulcers via modulating Nrf2/HO-1 and HMGB-1/NF-κB trajectories. This highlights the potential of red onion peels in treating gastric ulcers.

Ipomoea pes-tigridis L. extract accelerates wound healing in Wistar albino rats in excision and incision models

Ethnopharmacological relevanceAn annual herb, Ipomoea pes-tigridis L. (Convolvulaceae) is widely used for its anti-inflammatory and anti-spasmodic properties in traditional medicine. As well as treating wounds, fever, skin disorders, and other ailments, it is also used for other purposes. Aim of the studyThis study investigated polyphenolic content, antioxidant activity, RP-HPLC, wound healing, and antioxidant enzyme activity. In terms of I. pes-tigridis potential for healing wounds, there is no scientific data available. Hence this study is designed to use animal models to investigate the ethnopharmacological report. Materials methodsThe crude extracts of stem and leaf were subjected to phytochemicals, TPC, TTC, TFC, and free radical scavenging assays (DPPH, ABTS, etc). Excision and incision models were used to assess wound healing using the screened extracts (IPLEA, IPLM, IPSEA, and IPSM). Various tissue parameters (hydroxyproline, hexosamine, hexuronic acid content), as well as antioxidant enzyme activity (SOD, Catalase, GPX, LPO), were also examined. ResultsThe maximum amount of polyphenolic content was found in IPLM (TPC- 118.86 ± 5.94 mg GAE/g, TTC - 75.25 ± 2.64 mg TAE/g, and TFC-25.73 ± 0.99 mg GAE/g) with significant IC50 value of 1.65 ± 0.87 μg/mL among all the extracts. Coumaric acid was reported high (92.86 mg/g) in RP-HPLC analysis of crude extract in IPLEA. The in vivo excision wound healing model revealed that 1% IPLM had better healing property with the maximum wound healing area (0.098 ± 0.03 cm) and wound concentration (95.56 ± 1.95%) was reported with the significance level of ***P < 0.001, **P < 0.01, *P < 0.05. In the incision model, IPLM represented maximum tensile strength (27500 gf). A significant functional effect of the granulation tissue parameters and enzyme antioxidants on the wound-healed area of dry tissue was also observed. Finally, the histopathological analysis showed enhanced re-epithelialization, fibroblast proliferation, and collagen synthesis in wound-treated animal tissue in both models. ConclusionAccording to the present study, antioxidant-rich I. pes-tigridis promotes healthy cell regeneration while reducing inflammation and oxidative stress for wound healing. Additionally, it also enhances circulation and promotes healing.

Molecular phylogeny and morphological studies reveal a 30‐year‐old rain tree (Samanea saman) maintains populations of Meloidogyne enterolobii, a new host plant in Thailand

AbstractSamanea saman (Jacq.) Merr., commonly known as rain tree, is a crucial shade tree with traditional medicinal properties. In our study, 50 soil samples were collected around the rhizosphere of rain tree in Bangkok, Pathum Thani, Uttaradit, Khon Kaen and Ubon Ratchathani Provinces in Thailand. Meloidogyne populations were only found in soil samples from Mueang district, Khon Kaen Province and a 30‐year‐old rain tree exhibited root gall symptoms caused by root‐knot nematodes (Meloidogyne) on its adventitious roots. The species of the nematodes were identified based on its morphological and molecular characteristics. The results demonstrated that the perineal patterns and morphometrics of the nematodes were highly similar to the original description of M. enterolobii, except for a squarish dorsal arch and two faint lateral lines. During the molecular analysis, nematode DNA was extracted and amplified using three primer sets, C2F3/1108, D2A/D3B and MK7F/MK7R SCAR primer, to generate polymerase chain reaction (PCR) products of 700, 750 and 520 bp, respectively. The polygenetic tree of the two markers displayed similarity among the COII and the 16S rRNA gene in mitochondria and the D2/D3 region in 28S rRNA. Therefore, the nematodes were identified as M. enterolobii Yang and Eisenback, 1983. This study reports a new host record of M. enterolobii on rain tree.

Assessment of antimicrobial and anthelmintic activity of silver nanoparticles bio-synthesized from Viscum orientale leaf extract

BackgroundViscum orientale is a largely used parasitic plant with traditional medicinal properties. They are considered to possess the medicinal properties of host tree which they grow on. It’s a least explored plant with ethanopharmacological importance. As a result, the current work aimed to investigate the biological effects of Viscum orientale extract and silver nanoparticles (AgNPs) generated from it.MethodsAgNPs synthesized using Viscum orientale plant extract and analysed on time dependent series and was characterized using Ultra Violet UV–visible spectra, Fourier Transform Infrared Spectroscopy FTIR, X-ray diffraction (XRD), Energy Dispersive X-ray Spectroscopy (EDX), Scanning Electron Microscopy (SEM). Further using disc method anti-microbial assay was performed following antioxidation screening using 1,1-diphenyl-2-picryl-hydrazyl (DPPH), reducing power and nitric oxide content and heamgglutination with human blood.ResultsOn green synthesis using silver, the phyto contituents of plant Viscum orientale effectively reduced silver ions at 3-4 h of continuous stirring to form AgNPs. UV–vis spectra showed a typical peak of AgNPs at 480 nm. The FTIR analysis confirmed the covering of silver layers to bio-compounds of the extract. SEM analysis represented AgNPs as spherical morphologies ranging from 119–222 nm. AgNPs exhibited impressive zone of inhibition against Escherichia coli (8.1 ± 0.3 mm), Staphylococcus aureus (10.3 ± 0.3 mm), Bacillus subtilis (7.3 ± 0.3 mm), Bacillus cereus (8.2 ± 0.3 mm), Salmonella typhi (7.1 ± 0.2 mm). AgNps exhibited efficiency against DPPH at EC50 value of 57.60 µg/ml. and reducing power at EC50 of 53.42 µg/ml and nitric oxide scavenging of EC50 of 56.01 µg/ml concentration. Further, anthelmintic activity results showed synthesized nanoparticles significant reduction in the paralysis time to 5.4 ± 0.3 min and death time to 6.5 ± 0.6 min in contrast to the individual factors. On hemagglutination using AgNPs, above 80 µg/ml of concentration showed very significant effect on comparison with water extract.ConclusionSynthesized AgNPs using Viscum orientale water extract displayed versatile biological activity than individual extract. This study has forecasted a new path to explore more on this AgNPs for further research.

Comparative Study of&amp;lt;i&amp;gt; Mbuja &amp;lt;/i&amp;gt;and&amp;lt;i&amp;gt; daddawa &amp;lt;/i&amp;gt;Oils in View of Their Use in the Treatment of Some Chronic Diseases

<i>Mbuja</i> and <i>daddawa</i> are food condiments obtained by traditional uncontrolled fermentation of <i>Hibiscus sabdariffa </i>and<i> Parkia biglobosa </i>seeds respectively in African countries (Burkina Faso, Mali, Niger, Nigeria, Cameroon and Sudan etc...). These condiments are known for their nutritive values and for their health properties in traditional medicine to cure some chronic diseases. In spite of their nutritional and healthy properties the consumption of <i>Mbuja </i>and<i> daddawa </i>are less appreciated in urban areas. This is due to their strong smell, to their bad condition of manufacturing practices which leads to the rapid alteration of nutraceutic values. The main problem now is how to lead people to consume these condiments which nevertheless contains bioactive molecules, and can help in the treatment or in the prevention of some cardiovascular diseases. Among those bioactive molecules, the lipidic is one of the main element which has an impact on some chronic diseases. The fact that <i>daddawa</i> and <i>mbuja</i> are used for the same treatment, means that oils of both condiments could have same contents. For this reason the goal of this study target the comparative analyses of bioactive compounds of <i>daddawa</i> and <i>mbuja</i> oils that can justify the used of both condiments in the treatment of chronic diseases. To overcome this, the physicochemical composition of different oils were assessed by using classical methods. The results obtained show similitude values in colour, acid index, iodine value, saponification value, crude phenolic compounds, tannin as well as SFA, MUFA, PUFA. Based on these results, the physicochemical composition of <i>daddawa</i> and <i>mbuja</i> seem to be identic and can be applied for the same treatment.

Education on Giving Leaflets and The Potential of Karamunting Leaves (Rhodomyrtus Tomentosa) Herbal Efficacious in Society

Coronavirus disease (COVID-19) is spreading rapidly throughout the world including Indonesia. The use of herbal medicinal plants has increased since the Corona Virus Disease (COVID-19) pandemic, one of which is karamunting leaves. The methods used include: Field observations, we recorded the number of residents, their work and the plants that grow in the yard so that they can provide education to the community, especially in the North Landasan Ulin Village, Lianganggang District, Banjarbaru City. The results of the initial observation activities were carried out to look for problems in the community, the problems encountered by the community service team were the lack of public knowledge about plants that have the potential for traditional medicinal properties. Planting in the yard of the house has a characteristic as an herbal medicine. The conclusion from this activity was that education and giving leaflets for karamunting plants received a positive response from the community, after being given materials and leaflets as well as interactive discussions, the community realized the importance of using herbal medicines as an alternative treatment and increasing body immunity.

Bamboo leaf: A review of traditional medicinal property, phytochemistry, pharmacology, and purification technology.

Bamboos are perennial evergreen plants that belong to the subfamily Bambusoideae of the true grass family Poaceae, with more than thousands of species distributed around the world. They are used as a traditional medicine with demonstrated effects of anti-oxidation, free radical scavenging, anti-inflammatory, liver protection and ameliorating cognitive deficits. Bamboo leaf is mainly used for the treatment of atherosclerotic, diabetic and nervous system diseases. This review aims to provide up-to-date information on the traditional medicinal properties, phytochemistry, pharmacology, and purification technologies of bamboo leaf. Relevant information on bamboo leaf was obtained by an online search of worldwide accepted scientific databases (Web of Science, ScienceDirect, Elsevier, SpringerLink, ACS Publications, Wiley Online Library and CNKI). More than 100 chemical compounds, including flavonoids and flavonoid glycosides, volatile components, phenolic acids, polysaccharide, coenzyme Q10, phenylpropanoid and amino acids have been reported to be present. These compounds were usually extracted by column chromatography and membrane separation technologies. Preparative high performance liquid chromatography (PHPLC), high-speed counter-current chromatography (HSCCC), simulated moving bed chromatography (SMB) and dynamic axial compression chromatography (DAC) were the advanced separation technologies have been used to isolate C-glycosides from bamboo leaf flavonoid, the main bioactive ingredient of bamboo leaf. Currently, bamboo leaf is mainly used for the treatment of atherosclerotic, diabetic, hepatic diseases and nervous system related symptoms, which are attributed to the presence of bioactive components of bamboo leaf. Phytochemical and pharmacological analyses of bamboo leaf have been revealed in recent studies. However, most of the pharmacological studies on bamboo leaf have focused on bamboo leaf flavonoids. Further studies need to pay more attention to other phytochemical components of bamboo leaf. In addition, there is lack of sufficient clinical data and toxicity studies on bamboo leaf. Therefore, more clinical and toxicity researches on this plant and constituents are recommended.

Hippophae rhamnoides L.: A Comprehensive Review on the Botany, Traditional Uses, Phytonutrients, Health Benefits, Quality Markers, and Applications

Hippophae rhamnoides L. (sea buckthorn), consumed as a food and health supplement worldwide, has rich nutritional and medicinal properties. Different parts of H. rhamnoides L. were used in traditional Chinese medicines for relieving cough, aiding digestion, invigorating blood circulation, and alleviating pain since ancient times. Phytochemical studies revealed a wide variety of phytonutrients, including nutritional components (proteins, minerals, vitamins, etc.) and functional components like flavonoids (1-99), lignans (100-143), volatile oils (144-207), tannins (208-230), terpenoids (231-260), steroids (261-270), organic acids (271-297), and alkaloids (298-305). The pharmacological studies revealed that some crude extracts or compounds of H. rhamnoides L. demonstrated various health benefits, such as anti-inflammatory, antioxidant, hepatoprotective, anticardiovascular disease, anticancer, hypoglycemic, hypolipidemic, neuroprotective, antibacterial activities, and their effective doses and experimental models were summarized and analyzed in this paper. The quality markers (Q-markers) of H. rhamnoides L. were predicted and analyzed based on protobotanical phylogeny, traditional medicinal properties, expanded efficacy, pharmacokinetics and metabolism, and component testability. The applications of H. rhamnoides L. in juice, wine, oil, ferment, and yogurt were also summarized and future prospects were examined in this review. However, the mechanism and structure-activity relationship of some active compounds are not clear, and quality control and potential toxicity are worth further study in the future.

Methanolic fraction of Cassia fistula L. bark exhibits potential to combat oxidative stress and possess antiproliferative activity

ABSTRACT Cassia fistula L. is well known for its traditional medicinal properties as an anti-inflammatory, hepatoprotective, antifungal, antibacterial, antimutagenic, and wound healing agent. The aim of the present study was to determine antioxidant, genoprotective, and cytotoxic potential of different fractions of C. fistula bark including hexane (CaMH), chloroform (CaMC), ethyl acetate (CaME), and methanol (CaMM). Among all the fractions studied, CaMM exhibited maximal radical scavenging activity in antioxidant DPPH assay, Superoxide anion radical scavenging assay and nitric oxide radical scavenging assay displayed an IC50 value of 18.95, 29.41, and 13.38 µg/ml, respectively. CaMM fraction possessed the highest phenolic (130.37 mg gallic acid equivalent/g dry weight of extract) and flavonoid (36.96 mg rutin equivalent/g dry weight of fraction) content. Data demonstrated significant positive correlation between polyphenol levels and radical scavenging activity. Single cell gel electrophoresis (Comet assay) exhibited genoprotective potential of C. fistula bark fractions against DNA damage induced by hydrogen peroxide (H2O2) in human lymphocytes. CaMM fraction displayed highest protective ability against H2O2 induced-toxicity as evidenced by significant decrease in % tail DNA content from 30 to 7% at highest concentration (200 µg/ml). CaMM was found to be rich in catechin, gallic acid, chlorogenic acid, and kaempferol. The phenolic content and antioxidant ability of the fractions was markedly negatively correlated with H2O2- induced DNA damage in human lymphocytes. Cytotoxic potential was evaluated against dermal epidermoid carcinoma (A431), pancreatic (MIA PaCa-2) and brain glioblastoma (LN-18) cancer cell lines using MTT assay. Results showed that C. fistula bark fractions possessed highest toxicity against the skin carcinoma cells. CaMM fraction reduced over 50% cell growth at the concentration of 76.72 µg/ml in A431 cells. These findings suggest that fractions of C. fistula bark exhibit potential to be considered as therapeutic agents in various carcinomas.

Phytochemical Characterization and Antioxidant Potential of Latex Extracts of Cardiotoxic Plants - &lt;I&gt;Cascabela thevetia&lt;/I&gt; (L.) Lippold and &lt;I&gt;Plumeria alba&lt;/I&gt; L.

The Apocynaceae family has been noted for their traditional and conventional medicinal properties for ages. The characteristic feature of this family is the milky sap or latex that is present in almost all the species. Many of the plants belonging to this family are a rich source of important drugs and are known for their pharmacological properties. Among the pharmacological properties, the antioxidant property is one that is known to protect against many chronic diseases including heart diseases and cancer. Two plants of the family i.e., Cascabela thevetia (L.) Lippold and Plumeria alba L. have been considered in the present experiment. Globally much research work has been conducted based on the different parts of these plants but, very little data is available on the latex portion. Hence in the present study, an attempt has been made to evaluate the phytochemical constituents present in the plants. Further, the total phenolic and flavonoid content and their correlation with the antioxidant potential have been evaluated.