Use Of Promoters Research Articles

Review of floor impact sound insulation performance measurement evaluation methods and timber floor specifications in Japan

Since the 1970s, heavy-weight floor impact noise has been a concern in Japan, prompting the development of unique measurement and evaluation methods standardized by JIS (Japanese Industrial Standards). JIS A 1418-1 specifies the measurement method for light-weight floor impact sound insulation performance, while JIS A 1418-2 defines the measurement method for heavy-weight floor impact sound insulation performance. Two types of standard impact sources, namely car-tire impact source and rubber ball impact source, are specified for measuring the heavy-weight floor impact sound insulation performance. JIS A 1419-2 defines the evaluation method for floor impact sound insulation performance. These methods differ from the international ISO standards, making performance comparison challenging. This paper provides an overview of these Japanese methods. Furthermore, since the enforcement of "the Act on Promotion of Use of Wood in Buildings to Contribute to the Realization of a Decarbonized Society," construction of timber buildings has been promoted in Japan with the aim of encouraging wood usage and contributing to the SDGs (Sustainable Development Goals). However, the floor impact sound insulation performance of timber floors tends to be low, with various specifications for timber floor cross-sections differing among private companies. This paper introduces these specifications and also compares driving-point impedances.

Engineering a carbon source-responsive promoter for improved biosynthesis in the non-conventional yeast Kluyveromyces marxianus

Many desired biobased chemicals exhibit a range of toxicity to microbial cell factories, making industry-level biomanufacturing more challenging. Separating microbial growth and production phases is known to be beneficial for improving production of toxic products. Here, we developed a novel synthetic carbon-responsive promoter for use in the rapidly growing, stress-tolerant yeast Kluyveromyces marxianus, by fusing carbon-source responsive elements of the native ICL1 promoter to the strong S. cerevisiae TDH3 or native NC1 promoter cores. Two hybrids, PIT350 and PIN450, were validated via EGFP fluorescence and demonstrated exceptional strength, partial repression during growth, and late phase activation in glucose- and lactose-based medium, respectively. Expressing the Gerbera hybrida 2-pyrone synthase (2-PS) for synthesis of the polyketide triacetic acid lactone (TAL) under the control of PIN450 increased TAL more than 50% relative to the native NC1 promoter, and additional promoter engineering further increased TAL titer to 1.39 g/L in tube culture. Expression of the Penicillium griseofulvum 6-methylsalicylic acid synthase (6-MSAS) under the control of PIN450 resulted in a 6.6-fold increase in 6-MSA titer to 1.09 g/L and a simultaneous 1.5-fold increase in cell growth. Finally, we used PIN450 to express the Pseudomonas savastanoi IaaM and IaaH proteins and the Salvia pomifera sabinene synthase protein to improve production of the auxin hormone indole-3-acetic acid and the monoterpene sabinene, respectively, both extremely toxic to yeast. The development of carbon-responsive promoters adds to the synthetic biology toolbox and available metabolic engineering strategies for K. marxianus, allowing greater control over heterologous protein expression and improved production of toxic metabolites.

Case study of sound insulation performance of a first pure timber 3-story elementary school in Japan

In Japan, "the Act on Promotion of Use of Wood in Buildings to Contribute to the Realization of a Decarbonized Society" promotes the use of wood in buildings in general. The school buildings as public buildings are being promoted to be constructed with timber construction since timber school buildings effectively improve the educational environment. This paper presents the measurement results of the sound insulation performance in "the Uozu City Municipal Hoshinomori Elementary School", which is a first all-timber construction 3-story elementary school in Japan. By adopting a dry-type double floor structure for the floor finish structure and an independent ceiling for the ceiling structure, the floor impact sound insulation performances of the classrooms were Lr-60 for both heavy-weight floor impact sound and light-weight floor impact sound when evaluated using JIS A 1419-2. Moreover, the recommended reverberation time values of the Architectural Institute of Japan Environmental Standards were generally satisfied by installing dressed rockwool sound absorbing board on the ceiling.

Molecularly targetable cell types in mouse visual cortex have distinguishable prediction error responses

Predictive processing postulates the existence of prediction error neurons in cortex. Neurons with both negative and positive prediction error response properties have been identified in layer 2/3 of visual cortex, but whether they correspond to transcriptionally defined subpopulations is unclear. Here we used the activity-dependent, photoconvertible marker CaMPARI2 to tag neurons in layer 2/3 of mouse visual cortex during stimuli and behaviors designed to evoke prediction errors. We performed single-cell RNA-sequencing on these populations and found that previously annotated Adamts2 and Rrad layer 2/3 transcriptional cell types were enriched when photolabeling during stimuli that drive negative or positive prediction error responses, respectively. Finally, we validated these results functionally by designing artificial promoters for use in AAV vectors to express genetically encoded calcium indicators. Thus, transcriptionally distinct cell types in layer 2/3 that can be targeted using AAV vectors exhibit distinguishable negative and positive prediction error responses.

Older Patient Receptivity to the Integration of Patient Portals and Telehealth in Urogynecology: Promoters and Deterrents.

Limited studies focus on the integration of online portals, including telehealth services, in urogynecology while identifying promoters and deterrents of utilization, especially for the older population. This study aimed to identify facilitators, concerns, technical or personal issues encountered, and the desired features of the online patient portals among older urogynecology patients. This is a secondary analysis of a cross-sectional study of older patients (≥65 years). The survey was devised using 2 focus groups with questions addressing older patient practicality and comfort with virtual visits for menopause and urogynecology-specific conditions. A total of 205 patients completed the study. Promoters of use included health care professional encouragement, enrollment on site with concurrent education, and clarification of relevance of the virtual care to one's care. Patients who were uncomfortable with portal use reported anxiety and technical issues as deterrents for using such technology. More than half of the patients were comfortable having online visits for preoperative (51.7%), postoperative (66.3%), and medical management (73.7%). Up to 60.5% of the patients believed that virtual visits were equally stressful as in-person visits, whereas 24.4% believed that the logistics of in-person visits were the cause of stress. To improve access to care, augment the utilization of online patient portals, and combat ageism, enhancing the older urogynecologic patient's portal experience is vital. Investment in this population's needs includes education of patients, active enrollment, and engagement by health care systems, and addressing technical concerns.

Ethics of information and the metaverse

Recent promotion of use of the metaverse has raised concerns about the risks faced by users. These include fraud, identity theft, loss of privacy and online abuse. By considering the ethical issues we can begin to consider suitable avenues for regulation.

Characterization and engineering of the xylose-inducible xylP promoter for use in mold fungal species

Conditional promoters allowing both induction and silencing of gene expression are indispensable for basic and applied research. The xylP promoter (pxylP) from Penicillium chrysogenum was demonstrated to function in various mold species including Aspergillus fumigatus. pxylP allows high induction by xylan or its degradation product xylose with low basal activity in the absence of an inducer. Here we structurally characterized and engineered pxylP in A. fumigatus to optimize its application. Mutational analysis demonstrated the importance of the putative TATA-box and a pyrimidine-rich region in the core promoter, both copies of a largely duplicated 91-bp sequence (91bpDS), as well as putative binding sites for the transcription factor XlnR and a GATA motif within the 91bpDS. In agreement, pxylP activity was found to depend on XlnR, while glucose repression appeared to be indirect. Truncation of the originally used 1643-bp promoter fragment to 725 bp largely preserved the promoter activity and the regulatory pattern. Integration of a third 91bpDS significantly increased promoter activity particularly under low inducer concentrations. Truncation of pxylP to 199 bp demonstrated that the upstream region including the 91bpDSs mediates not only inducer-dependent activation but also repression in the absence of inducer. Remarkably, the 1579-bp pxylP was found to act bi-bidirectionally with a similar regulatory pattern by driving expression of the upstream-located arabinofuranosidase gene. The latter opens the possibility of dual bidirectional use of pxylP. Comparison with a doxycycline-inducible TetOn system revealed a significantly higher dynamic range of pxylP. Taken together, this study identified functional elements of pxylP and opened new methodological opportunities for its application.

Consistent Condom Use and Associated Factors among sexually active Military Personnel in Eastern Ethiopia: Cross-Sectional Study Design.

A condom is a latex-based device used to prevent pregnancy and sexually transmitted infections (STIs). Despite condom availability and promotion for use in STI prevention, consistent condom use remains too low in Ethiopia. A significant proportion of Ethiopian military personnel had multiple sexual partners, with lower rates of condom use with non-regular sexual partners. As a result, determining the pattern of condom use and the factors associated with it among military personnel is critical. The purpose of this study was to evaluate consistent condom use and associated factors among sexually active military personnel in Eastern Ethiopia from February 1 to 28, 2020. A cross-sectional study was conducted among 327 sexually active military personnel. To select the study participants, a systematic sampling technique was used. The data were entered into EpiData and exported to the Statistical Package for Social Sciences version 20 for analysis. To identify factors associated with condom use, bivariate and multivariable logistic regression analysis were used, and the strength of the association was measured using odds ratio and 95% confidence interval (CI), with P-values less than 0.05 considered statistically significant. The overall prevalence of consistent condom uses over one year by the respondents was 59.9% (95% CI: 54, 65). Study participants with an age of 30-35 years old (AOR = 3.12; 95% CI: 1.43, 11.38), above 35 years old (AOR = 2.42; 95% CI: 1.83, 9.30), college and above in educational status (AOR = 1.52; 95% CI: 1.20, 5.43), Officer in military rank (AOR = 3.12; 95% CI: 1.14, 10.15) and other military ranks (AOR = 3.08; 2.33, 8.52) were factors facilitate the use of condom consistently. In this study, two in every five military personnel use condoms inconsistently. Designing appropriate intervention and behavioral change strategies, as well as increasing the accessibility and availability of condoms around military camps, will undoubtedly have a significant impact on consistent condom utilization.

Riboflavin synthesis from gaseous nitrogen and carbon dioxide by a hybrid inorganic-biological system

Microbes can provide a more sustainable and energy-efficient method of food and nutrient production compared to plant and animal sources, but energy-intensive carbon (e.g., sugars) and nitrogen (e.g., ammonia) inputs are required. Gas-fixing microorganisms that can grow on H2 from renewable water splitting and gaseous CO2 and N2 offer a renewable path to overcoming these limitations but confront challenges owing to the scarcity of genetic engineering in such organisms. Here, we demonstrate that the hydrogen-oxidizing carbon- and nitrogen-fixing microorganism Xanthobacter autotrophicus grown on a CO2/N2/H2 gas mixture can overproduce the vitamin riboflavin (vitamin B2). We identify plasmids and promoters for use in this bacterium and employ a constitutive promoter to overexpress riboflavin pathway enzymes. Riboflavin production is quantified at 15 times that of the wild-type organism. We demonstrate that riboflavin overproduction is maintained when the bacterium is grown under hybrid inorganic-biological conditions, in which H2 from water splitting, along with CO2 and N2, is fed to the bacterium, establishing the viability of the approach to sustainably produce food and nutrients.

Evaluation of cytocompatibility and osteoconductivity of Zr-14Nb-5Ta-1Mo alloy with MC3T3-E1 cells.

The cytocompatibility and osteoconductivity of the Zr-14Nb-5Ta-1Mo alloy were investigated using a mouse osteoblastic cell line (MC3T3-E1) to promote the application of this newly developed alloy in dental/medical treatment. The initial cell-attached morphology was visualized by fluorescent staining, and cells cultured on the Zr alloy showed similar cell adhesion behavior to cells cultured on titanium (Ti). In our 5-day proliferation investigation, similar cell numbers were obtained with both Zr alloy and Ti. These results indicate that the cytocompatibility of Zr alloy is similar to that of Ti. In addition, the similar results in the evaluation of alkaline phosphatase (ALP) activity and staining of deposited calcium using alizarin red S with both Zr alloy and Ti indicated that the osteoconductivity of the Zr alloy is similar to that of Ti. Our results prove the good cytocompatibility and osteoconductivity of the Zr-14Nb-5Ta-1Mo alloy, enabling its promotion for use in dental/medical applications.

A Numerical Dynamic Analysis of a Multi-Body Bus

The promotion of use of enhanced the performant passenger transportation vehicles is a goal of governmental entities, as a way for a better environmental and sustainable life. Current trends of vehicle design and construction imply high safety requirements, as well as reduction of the environmental impact through lower energy consumption. The purpose of this work is to investigate the dynamic behavior, whether in-service or out-of-service, of a multi-body passenger bus articulated by a damping joint. The dynamic behavior of the chassis upon loading will be evaluated, and potential damage to the bus structure and the passenger safety will be evaluated. The numerical study is conducted to simulate a single-joint articulated urban bus model using Finite Element Method (FEM). Therefore, several computational analyses are performed in order to assess the influence of external loads on the natural frequencies of the structure.

FEATURES OF USING SMM FOR EFFECTIVE MANAGEMENT OF RESTAURANT BUSINESS ENTERPRISES

The article highlights the problem of using social networks, which is due to the active development of marketing, both worldwide and in Ukraine. Competition in the global market is intensifying, and therefore companies need to spend more money to promote their product so that it is not lost among many others. At the same time, the pandemic caused by Covid-19 has reduced marketing budgets. However, there is a growing need to find cost-effective and efficient ways to communicate with consumers. Businesses are encouraged to pay special attention to Internet marketing tools that promote the restaurant product, the restaurant business itself, and its brand through using social media, the content of which is created or updated through the efforts of owners and visitors, namely social media marketing. Competition is also intensifying, so only those companies that know and understand their consumers and competitors well, pursue effective communication policies, will succeed in the market. The shift of interests from mass to targeted marketing, as well as the dynamic development of communication channels and promotion tools have set new challenges for marketers. The article examines the existing methods of SMM promotion for use in the restaurant business, among which were targeted advertising, SEO promotion, interaction with users, competitions, advertising in communities. The main tasks of SMM, its advantages/disadvantages were highlighted. The main attention was paid to the method of evaluating the effectiveness of promotion and communication effectiveness of SMM for restaurant businesses. Several measures for the effective implementation of SMM in restaurants are proposed.

Energetic complexes as promoters for the green hypergolic bipropellant of EIL-H2O2 combinations

In this work, six energetic complexes were prepared and used as promoters for the hypergolic reaction of 1-ethyl-3-methylimidazolium cyanoborohydride [EMIM][BH3CN] and 90% H2O2. The structures of these compounds were studied by FT-IR spectroscopy, powder XRD, and single crystal X-ray diffraction. The results revealed that all the compounds were isostructural and each metal center was coordinated with four organic ligands and two counter ions. The decomposition temperatures of these compounds ranged from 169.6–255.9 °C. After adding 10 wt.% of prepared complexes to an ionic liquid, [EMIM][BH3CN], the shortest ignition delay time achieved was 94 ms. Moreover, the formed homogenous catalytic fuels had similar densities, thermal stabilities, as well as specific impulses similar to that of pure [EMIM][BH3CN]. The simple preparation method, excellent yield, as well as high performance of these compounds make them promising promoters for use in green hypergolic bipropellants.

A Rapid Pipeline for Pollen- and Anther-Specific Gene Discovery Based on Transcriptome Profiling Analysis of Maize Tissues.

Recently, crop breeders have widely adopted a new biotechnology-based process, termed Seed Production Technology (SPT), to produce hybrid varieties. The SPT does not produce nuclear male-sterile lines, and instead utilizes transgenic SPT maintainer lines to pollinate male-sterile plants for propagation of nuclear-recessive male-sterile lines. A late-stage pollen-specific promoter is an essential component of the pollen-inactivating cassette used by the SPT maintainers. While a number of plant pollen-specific promoters have been reported so far, their usefulness in SPT has remained limited. To increase the repertoire of pollen-specific promoters for the maize community, we conducted a comprehensive comparative analysis of transcriptome profiles of mature pollen and mature anthers against other tissue types. We found that maize pollen has much less expressed genes (>1 FPKM) than other tissue types, but the pollen grain has a large set of distinct genes, called pollen-specific genes, which are exclusively or much higher (100 folds) expressed in pollen than other tissue types. Utilizing transcript abundance and correlation coefficient analysis, 1215 mature pollen-specific (MPS) genes and 1009 mature anther-specific (MAS) genes were identified in B73 transcriptome. These two gene sets had similar GO term and KEGG pathway enrichment patterns, indicating that their members share similar functions in the maize reproductive process. Of the genes, 623 were shared between the two sets, called mature anther- and pollen-specific (MAPS) genes, which represent the late-stage pollen-specific genes of the maize genome. Functional annotation analysis of MAPS showed that 447 MAPS genes (71.7% of MAPS) belonged to genes encoding pollen allergen protein. Their 2-kb promoters were analyzed for cis-element enrichment and six well-known pollen-specific cis-elements (AGAAA, TCCACCA, TGTGGTT, [TA]AAAG, AAATGA, and TTTCT) were found highly enriched in the promoters of MAPS. Interestingly, JA-responsive cis-element GCC box (GCCGCC) and ABA-responsive cis-element-coupling element1 (ABRE-CE1, CCACC) were also found enriched in the MAPS promoters, indicating that JA and ABA signaling likely regulate pollen-specific MAPS expression. This study describes a robust and straightforward pipeline to discover pollen-specific promotes from publicly available data while providing maize breeders and the maize industry a number of late-stage (mature) pollen-specific promoters for use in SPT for hybrid breeding and seed production.

Alcohol use and associated factors among adolescent boys and young men in Kampala, Uganda

BackgroundAlcohol use leads to about 3 million deaths globally. The alcohol industry employs marketing strategies to establish their brands in the lives of young people at a time when addictive behaviors are initiated and reinforced. We conducted a survey among adolescent boys and young men (ABYM) to estimate the prevalence of alcohol use and associated factors using the Health Belief Model as the guiding framework.MethodsThe study was conducted among ABYM in- or out-of-school aged 10–24 years in Kampala, Uganda. We used questions adopted from the Global School-based Student Health Survey and the WHO STEPwise approach to Surveillance questionnaire to collect data. The outcome of interest was alcohol use within 30 days before the interview. We also asked about characteristics such as alcohol use by siblings, parents/ guardians, school status among others. We used odds ratios obtained via a logistic regression model as the measure of association.Results A total of 2500 ABYM participated, of which 262 (10.5 %, 95 %CI 9.3–11.7) had consumed alcohol within 30 days before the interview. Out-of-school ABYM had higher odds of consuming alcohol compared with their in-school counterparts AOR 1.55 (95 %CI 1.09–2.20). Compared with ABYM whose parents/ guardians did not drink alcohol, ABYM whose both parents consumed alcohol had higher odds of consuming alcohol AOR 2.24 (95 %CI 1.38–3.64) as were those with only a mother or female guardian who consumed alcohol AOR 1.95 (95 %CI 1.11–3.41). ABYM with siblings that drink alcohol had higher odds of consuming alcohol AOR 2.25 (95 %CI 1.80–3.52). ABYM who possessed items with an alcohol brand logo had higher odds of consuming alcohol AOR 2.00 (95 %CI 1.33–3.01).ConclusionsThere are significant levels of alcohol consumption among ABYM which calls for evidence-based measures targeting this age group to reduce consumption and recognizing the role of the family, school and community in prevention and promotion of use. There is need to regulate alcohol marketing and ensuring availability of alcohol dependence treatment services that build confidence among youth.

The first member of Exserohilum rostratum beneficial for promoting growth and yield of sunchoke (Helianthus tuberosus L.)

Helianthus tuberosus L. (sunchoke or Jerusalem artichoke) has been widely applied in food and industrial products. Due to its economical benefits, sunchoke has been of great interest in many areas. Therefore, the aims of this study were to isolate fungal endophytes from sunchoke and screen for their ability to produce IAA, GA3 and other plant growth-promoting (PGP) agents. Among a total of 126 isolates, 4 most effective PGP strains were identified as C. gloeosporioides CPS3/5, G. cingulate NMS1.4, E. rostratum NMS1.5 and Xylariaceae sp. KKL3. In the pot experiments, several plant growth parameters such as plant height, diameter of stem, SPAD chlorophyll content, plant biomass, plant root growth, the number of tubers and tuber inulin content in sunchoke inoculated with these fungal endophytes were significantly higher than those of the uninoculated controls. Outstandingly, E. rostratum NMS1.5 exhibited the best performance on promoting growth and yield of sunchoke. Interestingly, strain NMS1.5 is the first member of E. rostratum that is beneficial to plant growth without causing any negative effects. This study suggested that our fungal endophytes can be effective plant growth promoters for use as biofertilizers for sunchoke production.

SOIL ORGANIC MATTER (SOM): STATUS, TARGET AND CHALLENGES IN NEPAL

Changes in soil organic matter (SOM) are slow and difficult to monitor, usually apparent after few decades. Recent changes in the agriculture had its influence on soil, including the soil organic matter content. About 60% of soil in Nepal now have low organic matter content. Agriculture Perspective Plan (APP) was focused more on the concept of green revolution to increase the chemical fertilizer inputs, however, the scenario is changing. Use of organic fertilizers is promoted extensively by government and different organizations with a target of increasing SOM content from 1.92% in 2015 to 4% by 2035. This paper aims at analysing the current status, targeted goal and the challenges faced in the augmentation of the soil organic matter using data available. Achieving this target requires an addition of extra 2.244 ton/ha of organic matter on a yearly basis for 20 years. The average amount of organic matter (2.5-3 ton/ha) applied is lesser than a single season grain harvest. 4.69% of sites had high soil organic matter in fiscal year 075/76 which slipped to 2.64% in 2076/77. The sites with low soil organic matter increased from 12.73% to 15.31%. The causes behind the SOM decline varies according to different agro-ecological zones like soil erosion, residue burning, imbalanced fertilizer use, defective FYM production etc. Findings suggest precise technologies required to be adopted to tackle with the different niche specific causes of soil fertility decline. Despite the complete nutrient content, bulky nature of organic fertilizers sets a major drawback regarding their transportation, distribution and commercialization. Government of Nepal is promoting organic fertilizer use by subsidizing their production cost by 50%. Following integrated nutrient management (INM) techniques, sustainable soil management practices (SSMP) and promotion of use of locally available resources can play a huge role in making the technology sustainable to the farmers.

Rational design and testing of abiotic stress-inducible synthetic promoters from poplar cis-regulatory elements.

SummaryAbiotic stress resistance traits may be especially crucial for sustainable production of bioenergy tree crops. Here, we show the performance of a set of rationally designed osmotic‐related and salt stress‐inducible synthetic promoters for use in hybrid poplar. De novo motif‐detecting algorithms yielded 30 water‐deficit (SD) and 34 salt stress (SS) candidate DNA motifs from relevant poplar transcriptomes. We selected three conserved water‐deficit stress motifs (SD18, SD13 and SD9) found in 16 co‐expressed gene promoters, and we discovered a well‐conserved motif for salt response (SS16). We characterized several native poplar stress‐inducible promoters to enable comparisons with our synthetic promoters. Fifteen synthetic promoters were designed using various SD and SS subdomains, in which heptameric repeats of five‐to‐eight subdomain bases were fused to a common core promoter downstream, which, in turn, drove a green fluorescent protein (GFP) gene for reporter assays. These 15 synthetic promoters were screened by transient expression assays in poplar leaf mesophyll protoplasts and agroinfiltrated Nicotiana benthamiana leaves under osmotic stress conditions. Twelve synthetic promoters were induced in transient expression assays with a GFP readout. Of these, five promoters (SD18‐1, SD9‐2, SS16‐1, SS16‐2 and SS16‐3) endowed higher inducibility under osmotic stress conditions than native promoters. These five synthetic promoters were stably transformed into Arabidopsis thaliana to study inducibility in whole plants. Herein, SD18‐1 and SD9‐2 were induced by water‐deficit stress, whereas SS16‐1, SS16‐2 and SS16‐3 were induced by salt stress. The synthetic biology design pipeline resulted in five synthetic promoters that outperformed endogenous promoters in transgenic plants.

Efforts for promotion of Use of Radioisotopes (RI) -RI Experimental Protocols for Beginners-

Efforts for promotion of Use of Radioisotopes (RI) -RI Experimental Protocols for Beginners-

Tubaramure, a Food-Assisted Integrated Health and Nutrition Program, Reduces Child Wasting in Burundi: A Cluster-Randomized Controlled Intervention Trial

BackgroundLittle is known about the impact of food-assisted maternal and child health programs (FA-MCHN) on child wasting. ObjectivesWe assessed the impact of Tubaramure, a FA-MCHN program in Burundi, on child (0 to 24 months) wasting and the differential impacts by socio-economic characteristics and age. The program targeted women and their children during the first 1000 days and included 1) food rations, 2) strengthening and promotion of use of health services, and 3) behavior change communication (BCC). MethodsWe conducted a 4-arm, cluster-randomized, controlled trial (2010–2012). Clusters were defined as “collines” (communities). Impact was estimated using repeated cross-sectional data (n = ∼2620 children in each round). Treatment arms received household and individual (mother or child in the first 1000 days) food rations (corn-soy blend and micronutrient-fortified vegetable oil) from pregnancy to 24 months (T24 arm), from pregnancy to 18 months (T18), or from birth to 24 months (TNFP). All beneficiaries received the same BCC for the first 1000 days. The control arm received no rations or BCC. ResultsWasting (weight-for-length Z-score <2 SD) increased from baseline to follow-up in the control group (from 6.5% to 8%), but Tubaramure had a significant (P < 0.05) protective effect on wasting [treatment arms combined, −3.3 percentage points (pp); T18, −4.5 pp] and on the weight-for-length z-score (treatment arms combined, +0.15; T24, +0.20; T18, +0.17). The effects were limited to children whose mother and household head had no education, and who lived in the poorest households. The largest effect was found in children 6 to 12 months of age: the group with the highest wasting prevalence. ConclusionsFA-MCHN programs in highly food-insecure regions can protect the most disadvantaged children from wasting. These findings are particularly relevant in the context of the economic crisis due to the coronavirus disease 2019 pandemic, which is expected to dramatically increase child wasting.