Promising Donor Research Articles

Poly(2,5-bis(2-octyldodecyl)-3,6-di(furan-2-yl)-2,5-dihydro-pyrrolo[3,4-c]pyrrole-1,4-dione-co-thieno[3,2-b]thiophene): a high performance polymer semiconductor for both organic thin film transistors and organic photovoltaics

A new diketopyrrolopyrrole (DPP)-containing donor-acceptor polymer, poly(2,5-bis(2-octyldodecyl)-3,6-di(furan-2-yl)-2,5-dihydro-pyrrolo[3,4-c]pyrrole-1,4-dione-co-thieno[3,2-b]thiophene) (PDBF-co-TT), is synthesized and studied as a semiconductor in organic thin film transistors (OTFTs) and organic photovoltaics (OPVs). High hole mobility of up to 0.53 cm(2) V(-1) s(-1) in bottom-gate, top-contact OTFT devices is achieved owing to the ordered polymer chain packing and favoured chain orientation, strong intermolecular interactions, as well as uniform film morphology of PDBF-co-TT. The optimum band gap of 1.39 eV and high hole mobility make this polymer a promising donor semiconductor for the solar cell application. When paired with a fullerene acceptor, PC71BM, the resulting OPV devices show a high power conversion efficiency of up to 4.38% under simulated standard AM1.5 solar illumination.

Exacerbation of Oxidative Stress-Induced Cell Death and Differentiation in Induced Pluripotent Stem Cells Lacking Heme Oxygenase-1

Embryonic stem cells (ESCs) are promising donor sources in cell therapies for various diseases. Although low levels of reactive oxygen species (ROS) are necessary for the maintenance of stem cells, increased ROS levels initiate differentiation and cell damage. We and others have previously demonstrated that heme oxygenase (HO)-1, a stress response protein with antioxidative and anti-inflammatory properties, plays critical protective functions in cardiovascular and other diseases. However, the functions of HO-1 in ESCs remain to be elucidated. Our goal was to investigate the roles of HO-1 in ESC survival and differentiation. Due to the lack of HO-1-deficient ESCs, we used Oct3/4, Sox2, c-Myc, and Klf4 retroviruses to reprogram mouse embryonic fibroblasts into induced pluripotent stem (iPS) cells of different HO-1 genotypes. These iPS-HO-1 cells exhibited characteristics of mouse ESCs (mESCs) and formed teratomas that were composed of cell types of all 3 germ layers after injected into severe combined immunodeficiency mice. In response to oxidant stress, iPS-HO-1(-/-) cells accumulated higher levels of intracellular ROS compared with D3 mESCs or iPS-HO-1(+/+) cells and were more prone to oxidant-induced cell death. Spontaneous differentiation experiments revealed that Oct4 levels were significantly lower in iPS-HO-1(-/-) cells after leukemia inhibitory factor withdrawal and removal of feeders. Further, during the course of spontaneous differentiation, iPS-HO-1(-/-) cells had enhanced Erk1/2 phosphorylation, which has been linked to ESC differentiation. By the loss-of-function approach using iPS-HO-1(-/-) cells, our results demonstrate that a lack of HO-1 renders iPS cells more prone to oxidative stress-induced cell death and differentiation.

Optimization of liposomal lipid composition for a new, reactive sulfur donor, and in vivo efficacy studies on mice to antagonize cyanide intoxication.

Present studies have focused on a novel cyanide antidotal system, on the coencapsulation of a new sulfur donor DTO with rhodanese within sterically stabilized liposomes. The optimal lipid composition for coencapsulation of DTO with rhodanese is the combination of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine, cholesterol, cationic lipid (DOTAP), and 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] ammonium salt (with molar ratios of 82.7 : 9.2 : 3.0 : 5.1). With the optimized compositions, prophylactic and therapeutic in vivo efficacy studies were carried out in a mice model. When DTO was coencapsulated with rhodanese and thiosulfate the prophylactic antidotal protection was 4.9 × LD50. Maximum antidotal protection against cyanide intoxication (15 × LD50) was achieved with coencapsulated rhodanese and DTO/thiosulfate in combination with sodium nitrite. When applied therapeutically, 100% survival rate (6/6) was achieved at 20 mg/kg cyanide doses with the encapsulated DTO-rhodanese-thiosulfate antidotal systems with and without sodium nitrite. These data are indicating that the appropriately formulated DTO is a promising sulfur donor for cyanide antagonism.

Synthesis and Photovoltaic Performance of Two Triarylamine Organic Dyes Based on Truxene

The synthesis,characterization,and photovoltaic performance of two new organic dyes,hexapropyltruxeneamino-rhodanine-3-acetic acid(MXD8) and hexapropyltruxeneamino-3,4-ethylenedioxythiophene-rhodanine-3-acetic acid(MXD9),were reported.The two dyes contain truxene-based triarylamine as electron donor and a rhodanine-3-acetic acid as electron acceptor.It is found that the two dyes exhibit broad absorption and high molar absorption coefficients.The ground and the excited state levels of the two dyes were determined by using the cyclic voltammetry and the optical absorption spectroscopy.The results show the HOMO and LUMO levels of two dyes are suitable for dye-sensitized solar cells.The dyes were applied into nanocrystalline TiO2 dye-sensitized solar cells through standard operations.Under simulated AM 1.5 irradiation(100×10-3 W/cm2),MXD8 sensitized cell afforded a short-circuit photocurrent density(JSC) of 5.76×10-3 A/cm2,an open-circuit voltage(VOC) of 614 mV,and a fill factor(FF) of 0.66,corresponding to an overall conversion efficiency(η) of 2.33%.In contrast,though 3,4-ethylenedioxythiophene(EDOT) unit was introduced as the π-bridge,MXD9 sensitized cell gave a lower efficiency of 1.72%.A significantly larger extent of charge recombination between the injection electron and I2 and/or I-3 acceptor species was found for MXD9 dyes than MXD8 dyes based on the electrochemical impedance spectroscopy measurements,consequently,leading to a lower efficiency.These results suggest that the charge recombination may be correlated to the increased polarizability of the larger truxene-based triarylamine dyes.From our findings,we propose that the functionalized-truxene is a promising donor unit for the future development of organic sensitizers.

Phenotypic characteristics of hybrid cells generated by transferring neuronal nuclei into bone marrow stromal cell cytoplasts

Bone marrow stromal cells (BMSCs) are promising donor cells for transplantation therapies for a variety of diseases. However, there still lack efficient ways to induce directional differentiation of BMSCs to promote their practical use in transplantation therapy. In this study, we constructed hybrid cells by transferring neuronal nuclei into BMSC cytoplasts and investigated the proliferative capacity and phenotypic characteristics of the hybrid cells. The neuronal nuclei were labeled with Hoechst 33342 before the transfer process, and the cell membrane antigen CD71 was used as a marker of BMSC cytoplasts. The BMSC cytoplasts and neuronal karyoplasts were separated by Ficoll density gradient ultracentrifugation. The hybrid cells were generated by the polyethylene glycol-mediated fusion of BMSC cytoplasts with neuronal karyoplasts. The hybrid cells exhibited Hoechst 33342 staining in their nuclei and CD71 staining on their cytomembranes, which confirmed the success of cell fusion. The hybrid cells were positive for BrdU immunostaining. Viability analysis of the cultured hybrid cells by the MTT assay demonstrated their proliferative ability. Immunocytochemical staining revealed the expression of the neuron-specific markers NeuN and MAP2 in the third passage hybrid cells, which indicated their neuronal phenotypic characteristics. The results demonstrated that the hybrid cells produced by fusing neuronal karyoplasts with BMSC cytoplasts had proliferative capability and expressed the neuron-specific markers. Further study is required to investigate the phenotype of the hybrid cells both structurally and functionally.

Poly(3-hexylselenophene) solar cells: Correlating the optoelectronic device performance and nanomorphology imaged by low-energy scanning Transmission electron microscopy

The conjugated polymer poly(3-hexylselenophene-2,5-diyl) (P3HS) is a promising donor material for organic solar cells as its absorption covers a large part of the visible spectrum up to 730 nm. We investigate bulk heterojunctions of P3HS and the fullerene derivative [6,6]-phenyl C61-butyric acid methyl ester as the active layer in organic photovoltaic devices. Annealing of the respective samples leads to the formation of needle-like structures that we identify as P3HS whiskers by means of low-energy scanning transmission electron microscopy using a high-angle annular dark-field detector. A quantitative description of the size of the nanostructures was elaborated by means of spatial Fourier analyses. The results of electron microscopic studies are correlated with the macroscopic optoelectronic device properties, that is, solar cell performance parameters, with changes in the absorption spectrum and with the impact on the time-dependent device photoresponse. © 2011 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys, 2012

Photovoltaic Performance Improvement of D–A Copolymers Containing Bithiazole Acceptor Unit by Using Bithiophene Bridges

Two bithiophene-bridged D–A copolymers, PDTSBTBTz and PBDTBTBTz, based on bithiazole acceptor unit and dithienosiole (DTS) or benzodithiophene (BDT) donor unit, were synthesized by the Pd-catalyzed Stille-coupling reaction. The two copolymers exhibit good thermal stability, strong absorption in the visible region, and relatively lower HOMO energy level at ca. −5.10 eV. The hole mobilities of PDTSBTBTz and PBDTBTBTz measured by SCLC method are 1.85 × 10–3 and 1.77 × 10–3 cm2/(V s), respectively. Power conversion efficiency (PCE) of the polymer solar cell (PSC) based on PDTSBTBTz: PC70BM (1:1, w/w) was 3.82% with Jsc = 8.68 mA/cm2, Voc = 0.72 V, and FF = 0.611, under the illumination of AM1.5, 100 mW/cm2. In contrast, the PCE of the PSC based on PBDTBTBTz:PC70BM (1:1, w/w) reached 4.46% with Jsc = 9.01 mA/cm2, Voc = 0.82 V, and FF = 0.603. These results indicate that bithiophene-bridged D–A copolymers are promising photovoltaic donor materials for the application in PSCs.

Synthesis and properties of a novel squaraine dye modified by ferrocene

Abstract A novel squaraine dye (SQ) modified by ferrocene has been synthesized through (E)-1-dodecyl-2-ferrocenyl vinyl-1H-pyrrole and squaric acid. The molecular structure of SQ was characterized by 1H NMR, 13C NMR, MS and elemental analysis. SQ is high soluble in common solvents. The maximum absorptions of SQ in different solvents are in the range of 708−734 nm, exhibiting positive solvatochromism with increasing solvent polarity. The optical and electrical properties of SQ indicate that it is a promising electron donor material for bulk-heterojunction organic solar cell.

Lanthanide Complexes in FRET Applications

In this article, the main principles of Forster resonance energy transfer (FRET) are described with special emphasis on luminescent lanthanide complexes (LLCs) in modern fluorescence spectroscopy and microscopy and their use in biomolecular analysis. The theoretical fundamentals of FRET are explained and the unique photophysical properties of LLCs are presented. Their narrow emission bands, long excited-state lifetimes and large “Stokes shifts” make LLCs very promising energy donors for various FRET systems. Numerous recent literature examples as well as commercial applications, demonstrating the particular advantages of LLCs in FRET spectroscopy and microscopy, are reported. Afterwards, the benefits of multi-parametric FRET measurements using LLC donors are emphasized. The multiplexed approach allows the simultaneous detection of several analytes or binding events, which makes it especially important for modern high-throughput-screening as well as low-cost lab-on-a-chip devices. Finally, semiconductor quantum dots (QDs) and the advantages of their use as novel FRET acceptors are presented. A combination of LLCs and QDs in FRET provides extraordinary donor-acceptor pairs with outstanding properties, and long biomolecular distances of up to 20 nm become accessible. Extremely high sensitivity combined with multiplexed detection make the LLC-QD FRET pairs substantial for application in modern time-resolved fluoroimmunoassays (TR-FIA). Luminescent lanthanide complexes have been very efficient tools in various FRET applications and regarding the ever increasing amount of newly arising biomolecular questions and the development of novel fluorescent materials the future of LLC-based FRET is still wide open and highly promising.

Rational Design of Organic Asymmetric Donors D1–A–D2 Possessing Broad Absorption Regions and Suitable Frontier Molecular Orbitals to Match Typical Acceptors toward Solar Cells

A series of donors have been designed based on three building blocks, i.e., triphenylamine (TPA), 4,7-di(thiophen-2-yl)benzo[c][1,2,5]thiadiazole (DBT), and oligothiophenes (5Th), to investigate suitable molecules toward solar cell materials. The donors are built by two donor fragments (TPA as D1 and 5Th as D2) and one acceptor fragment (DBT as A) in three topologies with or without linkages and electron-withdrawing groups. Their electronic and optical properties have been characterized by the PBE0/6-31G(d,p) and the TD-PBE0/6-31+G(d,p) approaches. The calculated results reveal that the asymmetric donor in the topology of D1-A-D2 with vinyl linkage and -NO(2) groups is a rational strategy to design novel material possessing a small HOMO-LUMO gap, broad and intense absorption spectra, and higher charge transfer ability. The promising donors for [6,6]-phenyl-C(61)-butyric acid methyl ester (PCBM), bisPCBM, and PC70BM as acceptors are recommended theoretically for the first time for solar cells based on the proper match for frontier molecular orbitals between donors and acceptors.

Downwards tuning the HOMO level of polythiophene by carboxylate substitution for high open-circuit-voltage polymer solar cells

Three polythiophene derivatives with carboxylate substituents, PT–C1, PT–C2 and PT–C3 were synthesized by the Pd-catalyzed Stille-coupling method, for downwards tuning of the highest occupied molecular orbital (HOMO) of polythiophene. The polymers show similar visible absorption spectra to that of regioregular poly(3-hexylthiophene) (P3HT). By attaching the carboxylate substituent, the HOMO energy level downwards shifted to −5.15, −5.11 and −5.10 eV for PT–C1, PT–C2 and PT–C3, respectively, in comparison with that (ca. −4.8 eV) for P3HT. The polymer solar cells (PSCs) based on the three polymers as donor and PC70BM as acceptor all exhibit relatively high open circuit voltage (Voc) of ca. 0.8 V. The power conversion efficiency (PCE) of the PSCs based on PT–C3: PC70BM = 1 : 1.5 (w/w) reached 3.87% with Voc = 0.78 V, short circuit current (Jsc) of 9.68 mA cm−2 and fill factor (FF) of 51.2% under the illumination of AM1.5, 100 mW cm−2. These results indicate that attaching the carboxylate substituent is a simple and effective way to modulate energy levels of polythiophene derivatives and PT–C3 is a promising donor material for PSCs.

Hippocampal Stem Cell Grafting-Mediated Recovery of Injured Hippocampus in the Rat Model of Temporal Lobe Epilepsy

ABSTRACTHippocampal stem cells (HSCs) are considered promising donor cells to promote reorganization of degenerated regions of the injured hippocampus in the epileptic brain. However, the efficacy of HSC grafting for repairing injured hippocampus remains unclear. To address this issue, we transplanted neonatal rat HSCs into the right hippocampus in rats with kainite acid (KA)-induced epilepsy. The activity of the hippocampus and amygdala nucleus was monitored with electroencephalogram (EEG) throughout 24 weeks posttransplantation. Rats with grafted HSCs exhibited reduced frequency of epileptic wave discharge and a 50% decrease in the amplitude of discharge. At 1, 4, 8, and 24 weeks posttransplantation, the aberrant mossy fiber sprouting (MFS) was evaluated with Timm's stain and the number of CA3 pyramidal neurons was analyzed with Nissl staining. Aberrant MFS induced by KA-lesion was notably suppressed by HSC grafts beginning 4 weeks posttransplantation, and was most effective by 8 weeks. In addition, the loss of CA3 pyramidal neurons was partially restored and reached the most recovery at 8 weeks. Taken together, these results suggest that HSCs derived from the postnatal hippocampus offer a promising reparative effect on KA-induced epileptic brain.

Solution-Processable Low-Molecular Weight Extended Arylacetylenes: Versatile p-Type Semiconductors for Field-Effect Transistors and Bulk Heterojunction Solar Cells

We report the synthesis and characterization of a series of five extended arylacetylenes, 9,10-bis-{[m,p-bis(hexyloxy)phenyl]ethynyl}-anthracene (A-P6t, 1), 9,10-bis-[(p-{[m,p-bis(hexyloxy) phenyl]ethynyl}phenyl)ethynyl]-anthracene (PA-P6t, 2), 4,7-bis-{[m,p-bis(hexyloxy)phenyl]ethynyl}-2,1,3-benzothiadiazole (BTZ-P6t, 5), 4,7-bis(5-{[m,p-bis(hexyloxy)phenyl]ethynyl}thien-2-yl)-2,1,3-benzothiadiazole (TBTZ-P6t, 6), and 7,7'-({[m,p-bis(hexyloxy)phenyl]ethynyl}-2,1,3-benzothiadiazol-4,4'-ethynyl)-2,5-thiophene (BTZT-P6t, 7), and two arylvinylenes, 9,10-bis-{(E)-[m,p-bis(hexyloxy)phenyl]vinyl}-anthracene (A-P6d, 3), 9,10-bis-[(E)-(p-{(E)-[m,p-bis(hexyloxy)phenyl]vinyl}phenyl)vinyl]-anthracene (PA-P6d, 4). Trends in optical absorption spectra and electrochemical redox processes are first described. Next, the thin-film microstructures and morphologies of films deposited from solution under various conditions are investigated, and organic field-effect transistors (OFETs) and bulk heterojunction photovoltaic (OPV) cells fabricated. We find that substituting acetylenic for olefinic linkers on the molecular cores significantly enhances device performance. OFET measurements reveal that all seven of the semiconductors are FET-active and, depending on the backbone architecture, the arylacetylenes exhibit good p-type mobilities (mu up to approximately 0.1 cm(2) V(-1) s(-1)) when optimum film microstructural order is achieved. OPV cells using [6,6]-phenyl C(61)-butyric acid methyl ester (PCBM) as the electron acceptor exhibit power conversion efficiencies (PCEs) up to 1.3% under a simulated AM 1.5 solar irradiation of 100 mW/cm(2). These results demonstrate that arylacetylenes are promising hole-transport materials for p-channel OFETs and promising donors for organic solar cells applications. A direct correlation between OFET arylacetylene hole mobility and OPV performance is identified and analyzed.

Axon growth-promoting properties of human bone marrow mesenchymal stromal cells

Mesenchymal stromal cells are promising candidate donor cells for promoting functional tissue repair following traumatic spinal cord injury (SCI), however, the mechanism(s) of action remain poorly defined. Here, we describe an in vitro study of the axon growth-promoting properties of highly enriched populations of adult human mesenchymal stromal cells (hMSC). A random, non-oriented pattern of neuritic outgrowth was observed from dissociated adult rat DRG neurons seeded onto confluent A431 cells and PLL/laminin positive control substrata. Confluent hMSC formed arrays of similarly orientated cell bodies and processes which supported the regeneration of significantly more primary neurites but a slightly lower overall neuritic length than was observed over the PLL/laminin control substrate. The hMSC exerted a strong influence on the direction of neuritic outgrowth, with many regenerating processes following the orientation of underlying hMSC. The production of extracellular matrix appeared to be responsible for neuritic directionality, but the release of growth factors was a significant promoter for DRG neuritic outgrowth. This suggests that further investigations into the properties of hMSC may be of particular interest in the development of transplant-mediated strategies intending to promote functional axonal regeneration after SCI.

A Polybenzo[1,2‐b:4,5‐b′]dithiophene Derivative with Deep HOMO Level and Its Application in High‐Performance Polymer Solar Cells

Sun catcher: The new benzo[1,2-b:4,5-b′]dithiophene-containing polymer PBDTTBT exhibits a power conversion efficiency of up to 5.66 %. The high open-circuit voltage (0.92 V) originates from the low HOMO level of the polymer and the high internal and external quantum efficiencies over a wide spectral range. PBDTTBT is a promising donor material for application in polymer solar cells.

Genetic modification of pigs as organ donors for xenotransplantation

Transgenic pigs are promising donor organisms for xenotransplantation as they share many anatomical and physiological characteristics with humans. The most profound barrier to pig-to-primate xenotransplantation is the rejection of the grafted organ by a cascade of immune mechanisms commonly referred to as hyperacute rejection (HAR), acute humoral xenograft rejection (AHXR), immune cell-mediated rejection, and chronic rejection. Various strategies for the genetic modification of pigs facilitate tailoring them to be donors for organ transplantation. Genetically modified pigs lacking alpha-1,3-Gal epitopes, the major xenoantigens triggering HAR of pig-to-primate xenografts, are considered to be the basis for further genetic modifications that can address other rejection mechanisms and incompatibilities between the porcine and primate blood coagulation systems. These modifications include expression of human complement regulatory proteins, CD39, endothelial protein C receptor, heme oxygenase 1, thrombomodulin, tissue factor pathway inhibitor as well as modulators of the cellular immune system such as human TNF alpha-related apoptosis inducing ligand, HLA-E/beta-2-microglobulin, and CTLA-4Ig. In addition, transgenic strategies have been developed to reduce the potential risk of infections by endogenous porcine retroviruses. The protective efficacy of all these strategies is strictly dependent on a sufficiently high expression level of the respective factors with the required spatial distribution. This review provides an overview of the transgenic approaches that have been used to generate donor pigs for xenotransplantation, as well as their biological effects in in vitro tests and in preclinical transplantation studies. A future challenge will be to combine the most important and efficient genetic modifications in multi-transgenic pigs for clinical xenotransplantation.

The effect of donor age on progression of spermatogenesis in canine testicular tissue after xenografting into immunodeficient mice

The objective of this study was to examine the effect of donor age on progression of spermatogenesis in dog (Canis lupus familiaris) testis tissue after xenografting. In Experiment 1, canine testes were obtained by surgical castration. Based on developmental pattern of spermatogenesis at the time of grafting, donors were categorized as immature, young, and adult (<4, 4 to 6, and >6 mo old, respectively). Fragments of testis tissue were implanted subcutaneously on the back of immunodeficient mice; xenografts were retrieved and analyzed 4, 6, or 8 mo later. At 4 mo postgrafting, immature and young groups had higher graft recovery rates, graft weights, vesicular gland indices, seminiferous tubule numbers, and larger seminiferous tubular diameters compared with those of adult donor xenografts. At 8 mo postgrafting, immature donor xenografts had maintained growth and development as exhibited by greater graft weights, vesicular gland indices, seminiferous tubule numbers, and tubular diameters compared with those of adult donor xenografts. At this time point, growth and development of xenografts did not differ between immature and young donors, whereas those from young donors had greater seminiferous tubule numbers and diameters compared with those of adult donor xenografts. Elongated spermatids were the most advanced germ cell type present at 4 and 8 mo postgrafting in xenografts of immature age groups. In Experiment 2, the longer-term efficiency of spermatogenesis and the potential sperm production in xenografts from immature donor dogs were determined. Testis tissue from 2-mo-old donor dogs were grafted into recipient mice, and xenografts were retrieved after 13 mo. Complete spermatogenesis was present in 5 of 29 recovered xenografts, with isolation of fully formed sperm (up to 36.3 × 10 6 per gram tissue). In conclusion, immature and young donors (<6 mo of age) were the most promising donors for dog testis tissue xenografting. This strategy may offer an alternative for male germ-line preservation for canids that die prematurely or must be castrated before maturation.

Reduced Relapse and Similar Progression-Free Survival After Double Umbilical Cord Blood Transplantation (DUCBT): Comparison of Outcomes Between Sibling, Unrelated Adult and Unrelated DUCB Hematopoietic Stem Cell (HSC) Donors.

Abstract 662For patients with hematologic malignancies who lack a matched or mismatched unrelated donor, UCBT is an alternative. However, only a minority of larger adolescents and adults are able to find a single UCB with an adequate cell dose. Double UCB unit grafts (DUCB) have previously been shown to extend this HSC option. In order to understand the relative merits of each HSC source, we combined the datasets at the University of Minnesota and Fred Hutchinson Cancer Research Center. Between 2001- and 2008, 536 patients with leukemia (AML, n=211; ALL, n=236; CML, n=70 and MDS, n=19) were transplanted with HSC from a matched sibling (SIB, n=204), 8/8- (MUD, n=152) or 7/8-HLA (MMUD, n=52) matched unrelated donor or DUCB (n=128). All patients received myeloablative conditioning with cyclophosphamide 120 mg/kg and total body irradiation 1200-1320cGy with fludarabine 75mg/m2administered prior to DUCB. All patients received GVHD immunosuppression with a calcineurin inhibitor and either methotrexate (SIB, MUD and MMUD) or mycophenolate mofetil (DUCB). While patient weight and sex distribution and proportion with standard risk disease (STD, AML and ALL in CR1-2, and CML in CP1) were similar, DUCB pts were younger (median age 25 yrs, SIB 40 yrs, MUD 31 yrs, MMUD 31 yrs; p<.01). The proportion of AML and ALL was similar among groups, although more CML patients received a MUD or MMUD. Recipient CMV seropositivity was lower among MUD (44%; SIB 58%; MMUD 60; DUCB 62%, p<.01). The median follow-up of survivors was 3.1 yrs (0.3-8.1). The univariate point estimates at the stated timepoints and multivariate outcomes are summarized in the Table.Outcomes after SIB vs. MUD vs. MMUD vs. DUCBSIB* Ref.MUD* RR (95%CI)MVA p-value†MMUD* RR (95%CI)MVA p-value†DUCB* RR (95%CI)MVA p-value†Neutrophil Recovery at day 4598% 1.097% 0.62 (0.50-0.78). . <0.0198% 0.63 (0.46-0.86). . <0.0187%; 0.29 (0.23-0.37). . <0.01Platelet Recovery at day 10086% 1.081% 0.83 (0.64-1.09). . 0.1875% 0.71 (0.48-1.04). . 0.0845% 0.22 (0.17-0.29). . <0.01Grade II-IV aGVHD at day 10065% 1.080% 1.68 (0.32-2.14). . <0.0185% 2.17 (1.46-3.23). . <0.0160% 1.01 (0.76-1.35). . 0.94cGVHD at 2 year47% 1.043% 1.04 (0.75-1.46). . 0.8048% 1.24 (0.77-2.0). . 0.3726% 0.73 (0.47-1.13). . 0.16TRM at 3 years24% 1.014% 0.92 (0.53-1.60). . 0.7627% 1.83 (0.97-3.42). .0.0634% 2.86 (1.81-4.51). . <0.01Relapse at 5 years43% 1.037% 0.83 (0.58-1.19). . 0.3235% 0.68 (0.39-1.20). . 0.1915% 0.27 (0.16-0.47). . <0.01PFS at 5 years33% 1.048% 0.83 (0.62-1.11). . 0.2038% 1.04 (0.70-1.53). . 0.8551% 1.00 (0.73-1.37). . 0.99*Results are shown as pointwise estimates, relative risk (RR) and the 95% confidence interval. SIB group was the reference (Ref) group for the multivariate analysis (MVA).†P-value shown relates to multivariate analysis.In summary, after a Cy/TBI based myeloablation, DUCB is associated with slower hematopoietic recovery compared to other HSC sources. While incidence of acute GVHD was similar to that observed after SIB, DUCB was associated with less acute and trend toward less chronic GVHD relative to unrelated donors. Despite reduced risk of GVHD after DUCBT, risk of relapse was remarkably low while TRM was elevated, resulting in similar PFS. In the absence of a SIB donor, using either MUD or DUCB yield encouraging PFS and are promising donor options. These results support a) the front line use of HLA 0-2 antigen mismatched DUCB in patients with hematological malignancy, b) development of new strategies to enhance hematopoietic recovery after DUCBT, potentially reducing the risk of TRM, c) studies to understand why relapse rates are reduced with DUCBT and d) quality of life assessments long term between HSC groups, as there appears to be less risk of acute and chronic GVHD after DUCBT. Disclosures:No relevant conflicts of interest to declare.

GENERAL COMBINING ABILITY OF TEN STRAWBERRY CULTIVARS FOR RIPENING TIME, FRUIT QUALITY AND RESISTANCE TO MAIN LEAF DISEASES UNDER POLISH CONDITIONS

The aim of the study was to estimate the combining ability of 10 strawberry cultivars ('Camarosa', 'Darselect', 'Elsanta', 'Gariguette', 'Granda', 'Honeoye', 'Kent', 'Kimberly', 'Rosie' and 'Vikat') for 6 traits in order to evaluate their usefulness in the strawberry breeding program. The progenies obtained from 45 crosses (diallel cross design, Griffing's 4 method) were evaluated in the field experiment (randomized complete block design in 4 replicates with 15 plants per plot) located in the Pomological Orchard, Skierniewice, Central Poland. Observations across two seasons (2005 and 2006) of six traits: time of fruit ripening, marketable yield, fruit weight, fruit infection by grey mould and plant susceptibility to leaf diseases (leaf scorch caused by Diplocarpon earliana and powdery mildew caused by Sphaerotheca macularis) were recorded. Statistical analysis of data was carried out according to the fixed Griffing's model separately for each season. In both seasons GCA and SCA effects were significantly differentiated for almost all evaluated traits (excluding fruit susceptibility to gray mould in 2006). Variation of GCA effects was predominantly larger than variation of SCA effects for all traits over both years. Late ripening cultivar 'Vikat' showed the highest significantly positive GCA effect for ripening time (consistent across the both seasons). Most of the other cultivars exhibited moderately negative GCA effects for this trait. 'Granda' and 'Darselect' could be promising donors for fruit size - their GCA effects for this trait were significantly positive in both seasons. 'Kimberly' can transmit high degree of resistance to both pathogens D. earliana and S. macularis, 'Gariguette' - to D. earliana and 'Camarosa' and 'Kent' - to S. macularis.

Variation in salt tolerance within a Georgian wheat germplasm collection

Bread wheat Triticum aestivum L. possesses a genetic variation for the ability to survive and reproduce under salt stress conditions. Durum wheat (T. durum Desf.) is in general more sensitive in comparison to bread wheat, however, exceptions can be found showing the same extent of salt tolerance. Endemic wheats in general are characterised by a high adaptability to their environment. The level and variability of salt tolerance were assessed in a germplasm collection of 144 winter and spring wheat accessions from Georgia comprising Triticum aestivum L., T. durum Desf., T. dicoccon Schrank, T. polonicum L. and Georgian endemics: T. carthlicum Nevski, T. karamyschevii Nevski, T. macha Dekapr. et Menabde, T. timopheevii (Zhuk.) Zhuk. and T. zhukovskyi Menabde et Ericzjan. The accessions were tested for salt tolerance at the germination stage. Large variability in salt tolerance within the Georgian germplasm was found among the different wheat species. The endemic hexaploid winter wheat T. macha and the endemic tetraploid wheat T. timopheevii were among the most tolerant materials, thus presenting promising donors for salt tolerant traits in future breeding efforts for salinity tolerance in wheat.