Mold Proliferation Research Articles

Assessment of Fungal and Contamination of Ochratoxin A and Patulin in Foods Susceptible to Contamination in the Yangzhou Market, China

The conducive conditions of warm and humid climates can facilitate mold proliferation and subsequent mycotoxin production during food processing and distribution, thereby posing a potential risk to consumer health. However, there exists a significant lack of research regarding the diversity of molds and the presence of ochratoxin A (OTA) and patulin (PAT) in food products available in the Yangzhou market. This study was conducted to assess OTA contamination levels and fungal presence in 57 cereal-based food samples, as well as PAT contamination levels and fungal presence in 50 types of foods, including apples, hawthorn berries, pears, and their derivatives. Ochratoxin A (OTA) was detected in 17 out of 57 cereal-based food samples, with concentrations ranging from 0.93 to 32.69 μg/kg. The contamination rate was determined to be 31.48%, and no samples exceeded the established regulatory limits. Furthermore, seven apple products were identified as contaminated with patulin (PAT), exhibiting concentrations between 26.85 and 192.78 μg/kg. Additionally, three food samples derived from hawthorn showed PAT contamination levels ranging from 29.83 to 88.56 μg/kg. Through purification on potato dextrose agar (PDA) medium, observation of colony morphology, and analysis of internal transcribed spacer (ITS) sequences, a total of 35 fungal strains belonging to 13 genera were identified in cereal-based foods. The predominant genera in cereals included Talaromyces, Fusarium, Aspergillus, and Penicillium. Additionally, twelve fungal strains from five genera (Penicillium, Cladosporium, Aureobasidium, Curvularia, and Alternaria) were isolated and identified in fruits and their derivatives. The findings indicate that OTA and PAT toxins are one of the important risk factors that threaten consumer health. Furthermore, the contamination of some other toxigenic strains is also a matter of substantial concern, with potential implications for consumer health.

New Refined Experimental Analysis of Fungal Growth in Degraded Bio-Based Materials

When exposed to different building environmental conditions, bio-composite materials, such as hemp mortars, represent a risk of mold proliferation. This later plays a critical role in the biodeterioration of the materials when their physical properties are locally modified by the natural aging process. The primary objectives of the present work are first to assess the evolution of the surface of contaminated mortar; second, to investigate an accurate DNA extraction method that could be used for both bio-composite mortars and their fiber sources collected in situ; then, to understand the process of the proliferation of mold strains on both hemp shives and hemp mortar; and finally, to compare mold strains present in these phases to show their relationship to mold contamination and their impact on human health. In situ hemp mortar contamination behavior was investigated in the region of Pau (France) two months after hemp mortar application in extreme conditions (high humidity, low temperature, no aeration), which did not match the standard conditions under which hemp mortar must be used. The SEM observations and FTIR and pH analyses highlighted the decrease in pH level and the presence of organic matter on the mortar surface. DNA sequencing results showed that hemp shives were the main source of fungal contamination of hemp mortar. A mold population analysis showed that the most dominant phylum was Ophistokonta, which represented 83.6% in hemp shives and 99.97% in hemp mortar. The Acrostalagmus genus representatives were the most abundant, with 42% in hemp shives and 96% in hemp mortar. The interconnection between the mold strain characteristics (particularly the ability to grow in extreme environments) and the presence of hemp mortar was emphasized.

Effect of the shifting from multi-layer systems towards recyclable mono-material packaging solutions on the shelf-life of portioned semi-hard cheese

Packaging with recyclable plastic films could be an interesting alternative solution to extend shelf-life of portioned cheese. Two multi-layer materials of different types of one polymer (MonoMAP1 and MonoMAP2) with different barrier properties (O2: 13 and 20 cm3(STP)/(m2 × 24 h), 0 % RH; CO2: 65 and 92 cm3(STP)/(m2 × 24 h), 0 % RH) and coupled with modified atmosphere (MAP) were considered. Their performances in affecting cheese stability were compared to those of conventional multi-layer solutions under-vacuum (MultiVAC) and MAP (MultiMAP). Microbiological and physicochemical quality indicators were considered for a shelf-life study. In MonoMAP2, the initial loss of carbon dioxide and concomitant permeation of oxygen into the pack caused an earlier proliferation of molds with values of 4.7 log cfu g−1, leading to its inapplicability. In the other packaged samples, the development of rancid volatile compounds resulted the more sensitive quality indicator to be used for shelf-life assessment, with a critical threshold perceived by panelists around 520 mg/kg. Cheese packaged in MonoMAP1 showed a shelf-life associated to rancidity development of about one third lower than that of MultiVAC and MultiMAP ones.

New Acoustic Design for the Piscina Mirabilis Located nearby the Port of Misenum

Many heritage buildings from ancient Rome are being refurbished based on their original plan’s structure. One of them is the piscina mirabilis located nearby in Naples, which was a cistern used by the Romans to collect drinkable water for the navy waiting in the port of Misenum. The piscina mirabilis has similar architectural characteristics to a “cathedral”; however, its current precarious architectural state is the result of high levels of humidity that have caused the proliferation of mold on its vertical and horizontal surfaces over the centuries. Acoustic measurements were conducted inside the piscina mirabilis, highlighting an existing condition of the room being very reverberant, not suitable for occasional speech and conversations. The design proposed by the authors involves some mitigation solutions for the acoustics, mainly focused on controlling the low–medium frequencies and the realization of a restoration project consisting of a raised timber-floored walkway that runs along the perimeter walls, with the addition of water covering the existing floor as a natural element dominating the room volume, which represents the primary function of the building in antiquity. A waterfall was designed to be on the northern side wall. Acoustic studies were an important part of the refurbishment strategy, and a mitigation solution was devised to control medium–low frequencies by using inflated balloons of different sizes that were suspended from the ceiling vaults instead of widely used acoustic panels. The proposed strategy lowered the reverberation time by 3–4 s to accommodate a minimal level of conversational understanding. Such a solution is appropriate for this heritage building as well as other future conservation projects.

Boosting mold aerosol reduction and dehumidification performance in liquid desiccant systems using airborne ultrasound: An experimental study

Indoor mold exposure in hot-humid regions poses considerable risks to public health and food security. Ensuring safe, durable, and energy-efficient air disinfection is crucial for the air-conditioning field. While liquid desiccant air-conditioning (LDAC) systems eliminate condensation-related mold proliferation, their capability to combat airborne mold spores is limited due to the spores' robust resistance. This paper explores a novel airborne ultrasound-assisted liquid desiccant air-conditioning (US-LDAC) system. Experiments were conducted using culture-based methods to compare the system's disinfection capacities against Aspergillus niger spores, a desiccant-resistant species, under ventilation, liquid desiccation dehumidification, and airborne ultrasound-assisted liquid desiccant dehumidification modes. Moreover, the viability and morphological changes of mold spores exposed to airborne ultrasound, with and without liquid desiccants, were analyzed to verify the disinfection effectiveness and extrapolate the improving mechanisms. The results show that irradiating low-intensity airborne ultrasound (∼127 W, harmless mechanical waves) into the dehumidifier significantly increased the disinfection efficiency by 50 % and the dehumidification rate by over 90 % when airflow passed through the dehumidifier (∼0.4 s). The enhanced disinfection is attributed to ultrasonic cavitation, which facilitates the rupture of the desiccant-captured spores through microstreaming-induced shear forces. Notably, the improvements were more pronounced in humid air and with dilute desiccant solutions, suggesting that efficiency can be maintained with lowered desiccant concentrations. The US-LDAC system was particularly adept at removing mold aerosols (2.1–3.3 μm) which are easily inhaled. These findings propose a promising avenue for retrofitting existing LDAC systems to effectively reduce airborne molds and enhance dehumidification, thereby protecting indoor air quality.

Reusable Smart Lids for Improving Food Safety at Household Level with Programmable UV-C Technology

The worldwide food industry faces the multiple challenges of providing food security while also reducing environmental and health consequences. This requires transitioning to chemical-free techniques of preserving food with a long shelf life that emphasize human health. Even though millions of people are experiencing hunger, the substantial amount of food that is being wasted is impeding the advancement towards UN Sustainable Development Goal 12, which aims to reduce food waste by 50% by the year 2030. On the other hand, conventional food preservation techniques still frequently depend on chemical additives, which might give rise to persistent health issues and potentially undermine nutritional quality. This emphasizes the necessity for inventive, non-chemical remedies that prioritize both prolonged storage duration and the safety of food. Consumer storage conditions, which are the ultimate phase of the food chain, still generate substantial waste because of the proliferation of mold and bacteria on fruits and vegetables, which presents health hazards. Enhancing storage conditions and extending shelf life is important. Low-frequency ultraviolet (UV-C) light technology provides a non-thermal and highly efficient method for fighting foodborne microorganisms such as mold. This method renders pathogens inactive while maintaining product quality, providing a cost-efficient and easily available alternative. This study proposes the development of a programmable “Smart-Lid” SLID storage system that utilizes upcycled home base glass jars with UV-C light-emitting lids to prevent mold growth on various open food items, including milk- and sugar-based food, sauces, and possibly dry meals. The research seeks to assess the efficacy and potential influence of the SLID solution with UV-C light’s potential with programmable applications in this preserving environment at the home level.

Survival models to estimate time to visible mold growth on new paper-based food-contact materials under varying environmental conditions

Effective packaging solutions are pivotal in addressing sustainability challenges associated with food consumption. Sustainable packaging must not only reduce ecological impact but also ensure food quality preservation, minimize the risk of foodborne diseases, and contribute to mitigating the accumulation of plastic waste. While paper-based food packaging is environmentally friendly, it is susceptible to mold growth when exposed to specific humidity and temperatures, potentially leading to spoilage and safety concerns. This study introduces an innovative modeling approach employing survival analysis to estimate time to visible mold growth on paper-based materials used in food-contact packaging under varying environmental conditions. The objective is to establish an alternative statistical framework for assessing the impact of relative humidity and temperature on mold proliferation. This approach is particularly relevant to novel sustainable packaging materials in the food industry, especially when dealing with censored data. Survival heatmaps are developed to simplify the visualization of spoilage likelihood, measured by time to visible growth at a given confidence level. Although the survival model is proposed for paper-based materials, there are no theoretical limitations to its extension for use with other materials within and beyond the food industry.

Estimating Time of Mold Growth in an Indoor Environment

Mold growth (not spores) on surfaces indicates the presence of moisture in an indoor environment at some point in time. Mold proliferation is merely the direct consequence of moisture intrusion. Direct microscopical examination is the primary method to determine if mold or other fungi have grown on a particular surface. This analysis, while generally straightforward, provides the important answer to the question of whether mold growth is present. The growth can be described as the presence of vegetative structures (hyphae or mycelium) with or without associated spores or sporulating structures.

Investigation on the Mineral Configurations, Antioxidant Power, and Durability in Processed Tamarind (Tamarindus indica L.) Seed Powder

ABSTRACT: The pulp from the tamarind (Tamarindus indica L.) manufacturing sector disposes of the tamarind seed as waste materials. In the present investigation, processed Tamarindus indica L. seed powders' mineral compositions, antioxidant activities, and duration of shelf life have been investigated. Standard techniques were used to analyze the mineral compositions, antioxidant activity (Total Phenolic, Flavonoid content and 2,2-diphenyl-1-picrylhydrazyl) and shelf life (Total plate count (TPC), fungi, and mold) in processed tamarind powder at periodic intervals of 0, 2, 4, 6, and 8 months. The subsequent minerals were substantially more abundant in the roasted T.indica seed flour. The roasted T. India seed powder had high antioxidant powers of 19.83mg of TFC, 49.5mg of TPC, and 31 g/ml of 2,2-diphenyl-1-picrylhydrazyl (DPPH). The roasting method increases the best antioxidant potency and low glycemic index and glycemic load. The total plate counts of the processed T. India seed powder were within the legally permitted ranges of 107 cfu/g, and fungi and mold proliferation were at extremely low levels, so the powder could be used to make additional nutritional products. Roasted seed powder can be employed as a therapeutic food and as a food supplement because of its vital mineral elements and strong antioxidant potential.

Risk of Fungal Growth in Nearly Zero-Energy Buildings (nZEB)

Research on nearly zero-energy buildings has addressed mainly the aspects of energy saving or technical and economic optimization, while some studies have been conducted on comfort and indoor air quality. However, the potential problems that may arise in low-energy buildings during the operational phase, and especially the risk of fungal growth, which can deteriorate the indoor environment and pose a health risk to the occupants, are yet to be extensively investigated. The present work intends to analyze previous research on microbial contamination in zero-energy buildings in order to identify the possible risks that may lead to fungal formation and the possible strategies to prevent the proliferation of molds. The methodology is based on a systematic literature review and subsequent critical analysis to outline perspectives on this topic. The main results indicate that high envelope insulation and inadequate ventilation are the leading causes of fungal growth in energy-efficient buildings. The need for more detailed regulation in this area is also highlighted. The study’s outcomes underline the need for more attention to be paid to the design and management of zero-energy buildings, aiming to achieve the reduction in energy demands while ensuring the occupants’ well-being.

Metschnikowia pulcherrima as an efficient biocontrol agent of Botrytis cinerea infection in apples: Unraveling protection mechanisms through yeast proteomics

The results obtained in this study show that the Mp-30 strain of Metschnikowia pulcherrima is able to completely prevent Botrytis cinerea infection in apples, which is a major postharvest disease of fruits throughout the world. We have observed that although Mp-30 is able to rapidly colonize wounds, sequestrate iron and secrete antifungal compounds, other unknown mechanisms that occur in the early phase of the yeast-fungal interaction must be implicated in the biocontrol response. The main objective of this study was to identify the pathways involved in the mechanism of action of Mp-30 against B. cinerea in apples. Therefore, differentially accumulated yeast proteins in the presence/absence of B. cinerea on wounded apples were studied to elucidate Mp-30 biocontrol mechanisms and regulation at the protein level. A comparative proteomic analysis showed that 114 yeast proteins were increased and 61 were decreased. The Mp-30 antagonistic response mainly showed the increase of (1) gene expression and protein translation related proteins, (2) trafficking and vesicle-mediated transport related proteins, (3) pyruvate metabolism and mitochondrial proteins related to energy and amino acid production, (4) fatty acid synthesis, and (5) cell envelope related proteins. On the other hand, redox homeostasis, and amino acid and carbon metabolism were downregulated. Since there is no yeast growth enhancement associated with the presence of B. cinerea, such regulation mechanisms may be related to the reprogramming of metabolism, synthesis of new compounds and reorganization of yeast cell structure. Indeed, the results show that several pathways cooperate in restructuring the plasma membrane and cell wall composition, highlighting their major role in the antagonistic interactions for apple protection against gray mold proliferation. These results are of great interest since they provide a clear insight into the yeast mechanisms involved in B. cinerea inactivation during the first hours of contact in the wounded fruit. They shed light on the unknown yeast molecular biocontrol mechanisms.

Hygrothermal properties of insulation materials from rice straw and natural binders for buildings

Conversion agricultural straws to building insulation materials has attracted great interest due to their good hygrothermal properties and low carbon footprint. In this study, fully bio-sourced composite materials were designed based on rice straw and natural binders. Sodium alginate and chitosan were used as the binders. A crosslinking method was proposed to prevent the water-soluble issue of the composites using sodium alginate as the binder. The influence of fiber size, binder type and binder ratio on the hygrothermal properties of the composite materials made of rice straw were investigated. It was found that all the materials are insulating with their thermal conductivity values between 0.038 and 0.047 W/(m⋅K). The alginate-based composites had the higher water vapor permeability and moisture sorption capacity than the chitosan-based composites. Larger fiber size and lower binder ratio resulted in lower thermal conductivity and higher water vapor permeability. However, fiber size and binder ratio had a negligible effect on moisture sorption capacity of the composite materials. Additionally, the mold growth was investigated by exposing the composite materials to severe conditions, i.e., 93% RH and 97% RH at 20 °C. Mold proliferation was found to be easier and quicker in the surface of alginate-based composites compared to the chitosan-based composites.

Structural Home Defects Are the Leading Cause of Mold in Buildings: The Housing and Health Service Experience.

The study aimed to evaluate the contribution of building problems to mold proliferation in dwellings. We investigated 503 dwellings of patients suffering from respiratory diseases, whose attending physicians had requested a home inspection if the area of mold was equal to at least one square meter. After careful visual evaluation and basic environmental measurements performed by a trained technician, environmental issues were classified into building defects, accidental water damage, and condensation. Data analysis demonstrated that building defects were the pre-eminent cause of mold proliferation. Among the building defects, water infiltration through leaks in roofs or walls was the leading cause. These results highlight the need for health professionals managing patients with respiratory diseases to be able to request a home inspection, and for city health authorities to commission professionals who can focus on building problems and find ways to address them.

LAWS DEALING WITH FOOD SAFETY LAWS IN PAKISTAN

Biological, Chemical and Physical contamination of foods is a terrible danger to emerging civilizations' health and economic progress. The abundant literature on0foodborne infections, particularly diarrhea in0children, exclusively illustrates the increased disease0burden associated with foodborne0illness in developing nations. The presence of several0pathogens in a variety of foods is prevalent in Pakistan. Precise0numbers for foodborne0infections in Pakistan0are difficult to come by due to a lack of monitoring, surveillance, and infection control. Aflatoxin contamination and mold proliferation are mostly caused by poor0processing and storage0of milk, cereal0grains, and nuts. Numerous studies show that a wide range of foods is contaminated with heavy0metals. The increasing population restricts the economic potential of individuals and states by encouraging traders and manufacturers to purposely degrade food0commodities provided for sale in order to maximize profit at the expense of quality0and safety. As a result, a recent trend0of food adulteration, notably milk adulteration, creates an urgent concern for the government. This analysis is a concentrated effort to shed light on the current food0safety situation in0Pakistan. Data from0local and relevant international research will be provided to paint a clear picture of food safety0in Pakistan. It is advocated that a comprehensive food0safety infrastructure be created, constructed, and implemented in order to provide a safer food supply.

Postbiotics as Dynamic Biological Molecules for Antimicrobial Activity: A Mini-Review

Postbiotics, products, or metabolites secreted by living probiotic bacteria like teichoic acids, peptides, enzymes, peptidoglycans, polysaccharides, organic acids, and external cell proteins are said to be produced during the bacterial fermentation process. However, postbiotics may provide immunization, antioxidant, prevents inflammation, low cholesterolemic, antimicrobial, antagonistic obesity, contrast hypertensive, and diabetic retinopathy impacts. In the current review, we attempt to display the antimicrobial performance of postbiotics. In this regard, we considered microbial strains used as postbiotic sources and postbiotics as antimicrobial agents in food products. All databases such as Science Direct, Scopus, Pub Med, and Google Scholar were examined using the following keywords: “postbiotics”, “Antimicrobial activity”, “Anti-inflammatory”, and “Low cholesterolemic”. Further studies demonstrated that probiotics are fed special forms of fiber (prebiotic) molecules, indicating substances known as postbiotics. Furthermore, short-chain fatty acids (SCFAs) like acetate, propionate, and butyrate are among the comprehensively investigated postbiotics. The extraction and purification of these compounds are carried out by means of dialysis, centrifugation, and freeze-drying techniques. According to the gained results, postbiotics assist in improving host health by increasing certain physiological functions. Furthermore, postbiotics can be used to increment the useful lifetime of different foods, like dairy products. It has also been shown that manually adding postbiotics to such products prevents the growth and proliferation of molds and thus the spoilage caused by them. This inhibitory effect indicates the antimicrobial properties of these compounds. Finally, we will see significant advances in the biological preservation of products, especially in the food industry.

Effect of gamma radiation coupled to refrigeration on antioxidant capacity, sensory properties and shelf life of strawberries

The aim of this work was to perform a comprehensive study on the effect of gamma radiation on strawberries by evaluating its effect on physicochemical, microbiological, sensory acceptance, phenolic and flavonoid contents, and antioxidant activity over a storage period of 14 days at 4 °C. The results showed that gamma irradiation significantly decreased the proliferation of molds and yeasts at 1, 2 and 3 kGy up to seven days of storage. However, a significant decrease of firmness, redness and chroma was noticed with gamma radiation doses. Moreover, sensory scores revealed that gamma radiation had no significant effect on strawberries compared to the non-treated, the dose of 2 kGy seemed to be the best dose to maintain the acceptance sensory quality of strawberries for the panel. A positive relation between different physicochemical parameters and sensory descriptors was illustrated with Principal Compounds Analysis. The gamma radiation treatment and refrigerated storage indicated to have an increasing trend on the bioactive content of strawberries. Based on the obtained results, the dose of 2 kGy point out to be the optimal treatment to maintain the safety and global quality of strawberries with extended shelf-life.

Infrared Irradiation Drying Impact on Bee Pollen: Case Study on the Phenolic Composition of Eucalyptus globulus Labill and Salix atrocinerea Brot. Pollens

Bee pollen is commonly reputed as a rich source of nutrients, both for bees and humans. Its composition is well balanced and can be taken as a stand-alone food or as supplement, including for the elderly owing its low caloric value. However, storage conditions frequently lead to product degradation, namely due to the high moisture content that enable the proliferation of molds and bacteria. Herein, an infrared (IR)-based technology is proposed as a mean to determine moisture content, setting also a new scalable approach for the development of a drying technology to be used for bee pollen processing, which can be carried out in a short time, without impacting the phenolic and flavonoid content and associated bioactive effects. Proof-of-concept was attained with an IR moisture analyzer, bee pollen samples from Eucalyptus globulus Labill and Salix atrocinerea Brot. being selected as models. Impact of the IR radiation towards the phenolic and flavonoid profiles was screened by HPLC/DAD profiling and radical scavenging ability by the DPPH assay. The IR-based approach shows good reproducibility while simultaneously reducing drying time and energy consumption, thus implying a low environmental impact and being suitable for industrial scale-up once no degradation has been found to occur during the radiation process.

Current postharvest practices and aflatoxin contamination awareness amongst maize producers in Jimma Zone, Southwest of Ethiopia

Grain contamination by mycotoxins can cause significantly negative health and economic impact in areas where poor agricultural practices and food insecurity is prevalent. This study was conducted to investigate the current postharvest practices and aflatoxin contamination awareness level amongst maize producers in Jimma Zone, Ethiopia. Semi-structured questionnaires were used for quantitative and qualitative data collection from 90 randomly selected maize producers in two districts through a cross-sectional study design. The study revealed poor postharvest practices due to lack of proper infrastructure. Maize is harvested after maturation with the use of traditional sun drying. Plastic sheets are commonly used to protect harvested maize from rain during field drying. The majority of the respondents reported the use of traditional storage structures (81.1%) with great potential for possible mould proliferation and aflatoxin production. Maize producers of up to 62.2% were ignorant about aflatoxins and up to 26.7% reported the possible usage of aflatoxin contaminated maize in human food preparation and animal feed production. Up to 53.3% of the respondents were ignorant of aflatoxin risks in human health and stability during food processing hence the usage of mouldy maize fortella brewing. Further investigations on aflatoxin levels in maize-based food and beverages, and human exposure studies are needed. Moreover, there is a need to enhance the maize producers’ knowledge on good agricultural practices and mycotoxicosis through awareness programmes.

Hygro-thermal performance of an opaque ventilated façade with recycled materials during wintertime

This study investigates the thermo-hygrometric behaviour in wintertime of an open-joint ventilated façade, designed to be environmentally sustainable. It is the result of the Horizon Project “RE4 – REuse and REcycling of CDW materials and structures in energy efficient pREfabricated elements for building REfurbishment and construction” and it is composed by prefabricated elements made by recycled materials from construction and demolition waste. The designed façade has been installed in a full scale test-room located in Benevento (south Italy), with typical Mediterranean climate.The proposed analysis suggests that for the behaviour of the designed façade, the main influential factors are the solar radiation and the outdoor air temperature meanwhile the wind effect is negligible. Some mathematical correlations have been found and an empirical model that describes the air temperature in the cavity as a function of the external climatic parameters is developed. These findings could be useful for the assessment of performance of similar facades in the design phase but also for comparing the behaviour in different climates.All tests demonstrate that in winter time, in a humid and rainy climate, the prevalent effect is the insulation and the ventilation does not increase the heat losses. The designed system can effectively control the moisture content in the building envelope materials, ensuring dry surfaces and at the same time avoid the alteration of the thermo-hygrometric characteristics for the bacteria and mould proliferation, ensuring healthy environment and avoiding structural damage.

The effects of climate change on respiratory allergy and asthma induced by pollen and mold allergens.

The impact of climate change on the environment, biosphere, and biodiversity has become more evident in the recent years. Human activities have increased atmospheric concentrations of carbon dioxide (CO2 ) and other greenhouse gases. Change in climate and the correlated global warming affects the quantity, intensity, and frequency of precipitation type as well as the frequency of extreme events such as heat waves, droughts, thunderstorms, floods, and hurricanes. Respiratory health can be particularly affected by climate change, which contributes to the development of allergic respiratory diseases and asthma. Pollen and mold allergens are able to trigger the release of pro-inflammatory and immunomodulatory mediators that accelerate the onset the IgE-mediated sensitization and of allergy. Allergy to pollen and pollen season at its beginning, in duration and intensity are altered by climate change. Studies showed that plants exhibit enhanced photosynthesis and reproductive effects and produce more pollen as a response to high atmospheric levels of carbon dioxide (CO2 ). Mold proliferation is increased by floods and rainy storms are responsible for severe asthma. Pollen and mold allergy is generally used to evaluate the interrelation between air pollution and allergic respiratory diseases, such as rhinitis and asthma. Thunderstorms during pollen seasons can cause exacerbation of respiratory allergy and asthma in patients with hay fever. A similar phenomenon is observed for molds. Measures to reduce greenhouse gas emissions can have positive health benefits.