Proliferation Of Human Foreskin Fibroblasts Research Articles

Vapor-phase glutaraldehyde crosslinked waste protein-based nanofiber nonwovens as an environmentally friendly wound dressing

Vapor-phase glutaraldehyde crosslinking was used to improve the performance of feather keratin/gelatin (FK/Ge) nanofiber nonwovens for potential use as wound dressings. Crosslinking was performed by placing the electrospun nonwovens in a glutaraldehyde vapor for different times (1.5, 3, 4.5, 6, and 9 h). Fourier-transform infrared spectroscopy and X-ray diffraction analysis revealed strong interactions between FK and Ge, and verified the crosslinking of FK/Ge molecular chains by glutaraldehyde. Fibers in the crosslinked nonwovens formed a compact and porous structure that promotes cell proliferation and growth. Further, a proper extent of crosslinking reaction can effectively improve the mechanical strength and water resistance of FK/Ge nanofiber nonwovens, while maintaining their water uptake and thermal degradation properties within an acceptable range. These improvements are attributed to the flexible control over the degree of vapor-phase crosslinking. In vitro tests demonstrated that the crosslinked samples not only lack cytotoxicity but also promote the rapid proliferation of human foreskin fibroblasts. These new crosslinked nanofiber nonwovens derived from waste materials are promising for wound repair and care.

Effects of some cosmetic dyes and pigments on the proliferation of human foreskin fibroblasts and cellular oxidative stress; potential cytotoxicity of chlorophyllin and indigo carmine on fibroblasts.

Fibroblasts and fibroblast-like cells (FLCs) are highly distributed in the dermis layer of the skin and gastrointestinal tract. A few studies have investigated the effects of color additives of cosmetic products on human fibroblasts. Therefore, this in vitro study was conducted to investigate the effects of 9synthetic and natural dyes (indigo carmine, carmine, sunset yellow, tropaeolin, acid green 25, chlorophyllin, tartrazine, lissamine, and amaranth) on human foreskin fibroblast cells. MTT assay was applied to investigate the effects of dyes on human normal fibroblast cells. For investigating the possible mechanism of cytotoxicity, the effect of dyes was evaluated on parameters of cellular oxidative stress including lipid peroxidation and reactive oxygen species (ROS) as well as lactate dehydrogenase. In the MTT assay, a significant reduction (p≤0.05) was observed in the viability of fibroblast cells by chlorophyllin and indigo carmine at concentrations higher than 10 and 100 (μg/ml), respectively. Acid green 25 caused a significant reduction at very high concentrations (1000 and 2000μg/ml), which was not toxic. The effect of other investigated dyes was not significant on the fibroblasts. A trend in increased cell proliferation by amaranth and carmine was observed, but this enhancement was not statistically significant. No significant changes were observed in lipid peroxidation or lactate dehydrogenase. Chlorophyllin caused a significant increase in the production of cellular ROS. Indigo carmine and chlorophyllin had cytotoxicity on human fibroblasts, and thus, further studies are required on their safety of use in cosmetics and pharmaceuticals.

A new cytotoxic sesquiterpene lactone from Euphorbia microsphaera Boiss against human breast cancer (MCF-7) and human fibrosarcoma (HT1080) cells

A new guaianolide sesquiterpene lactone with cytotoxic properties was isolated from Euphorbia microsphaera Boiss. To determine the highest active fraction and isolate bioactive compounds, a bioassay guided fractionation approach was used. The general toxicity properties of the plant's extracts and fractions (fr1-10) were assessed against Artemia salina, Oryzeaphilus mercator, and Tribolium castaneum. Cytotoxic activities were investigated against normal human foreskin fibroblasts and two malignant cell lines, including human breast cancer (MCF-7) and human fibrosarcoma cells (HT1080) using the MTT assay at different time points of 24, 48, and 72 h. Single crystal X-ray diffraction (SC-XRD) and mass spectrometry data were used to determine the structure of the active guaianolide sesquiterpene lactone (3aR,4S,4aS,5R,7aS,9aS)-5-hydroxy-5,8-dimethyl-3-methylene-2-oxo-2,3,3a,4,4a,5,6,7,7a, 9a decahydroazuleno [6,5-b] furan-4-yl acetate (named aryanin). Chloroformic fraction 7 (fr7, LC50 = 93.50 μg/mL for general toxicity) had the highest toxicity result, with a mortality rate of more than 50% for both insect species after 12 h at 15 mg/mL. The highest cytotoxicity of aryanin was observed on 24 h treated MCF-7 with an IC50 of 13.81 μg/mL. After 24 h, the inhibition of MCF-7 cell proliferation was 92%–94% at concentrations of 25–50 μg/mL, respectively. On MCF-7, the IC50 was found to be 49.35 μg/mL after 72 h. This compound had a considerable cytotoxicity (IC50 ≤ 12.5 μg/mL, 24 h) on human foreskin fibroblasts. In contrast to the MCF-7 cell line, the proliferation of human foreskin fibroblasts was increased after 72 h.

A competitive nature-derived multilayered scaffold based on chitosan and alginate, for full-thickness wound healing

The aim of this study was to evaluate a bioactive multilayer wound dressing, based on chitosan and alginate. To enhance healing potential, Dracaena Cinnabari and Aloe Vera were loaded as separate layers into the scaffold. The bare and bioactive multilayered scaffolds were fabricated by an iterative layering freeze-drying technique. Following of topographical, chemical, and physical assessment, the performance of the scaffolds was evaluated in vitro and in vivo. The results revealed adequate attachment, and proliferation of human foreskin fibroblasts, indicating excellent biocompatibility of the bioactive scaffold. In vivo, the performance of the multi-layered scaffold loaded with the bioactive materials was comparable with Comfeel plus®. The wounds treated with the bioactive scaffold exhibited superior hypergranulation, fibroblast maturation, epithelization, and collagen deposition, with minimal inflammation, and crust formation. It is concluded that the synergism of extracellular matrix-mimicking multi-layered scaffolding with Aloe Vera and Dracaena Cinnabari could be considered as a supportive wound dressing.

Human Foreskin Fibroblast Behavior in the Presence of 3D Collagen, Fibrin, and Calcium Phosphate

3D modeling in cell‐based assays, provides an environment that mimics in vivo conditions. This allows for the examination of innate cellular behavior and aids in the development of next‐generation modeling and therapies. Cellular environments can be modeled using various constituents to manipulate the rigidity, porosity, and size of various 3D environments to observe cell characteristics in an environment similar to that from which they originated. The proliferation of Human Foreskin Fibroblasts (HFF‐1) were examined in a 3D collagen matrix with varying cell densities. Higher cell concentration within the 3D collagen matrix showed lower HFF‐1 cell proliferation. Furthermore, fibrin clots were used to mimic 3D microenvironments because of its biocompatibility and controllable biodegradability. Changing fibrinogen and thrombin concentrations provides a varying effect on the rigidity and porosity of a 3D environment and therefore affects the overall behavior of HFF‐1. There was an increase in fibrin bead size in the presence of cells with higher concentrations of fibrinogen and thrombin. To mimic a bone microenvironment, calcium phosphate (CaP) was introduced in increasing concentrations to support a rigid and structured environment. Increasing concentrations of CaP, resulted in an increase in the initial adhesion and a decrease in HFF‐1 proliferation over a 7‐day incubation period. Overall, cells in a 2D environment behave different than those in a 3D environment with varying components to imitate their in vivo conditions.Support or Funding InformationN/AThis abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.

Plectranthus tenuiflorus (Shara) Promotes Wound Healing: In vitro and in vivo Studies

The present study proved that bothPlectranthus tenuiflorus juice and essential oil exerted a healing promoting effect in rat wound model. The effect was shown to be mainly via their ability to stimulate fibroblasts proliferation in addition to an anti-bacterial effect of its thymol content. Leaves of the plant were collected from different regions. The whole leave juice or essential oil were extracted by chemical steam distillation method. Different concentrations were tested for their effects on the proliferation of human foreskin fibroblasts in tissue culture. Its efficiency in enhancing wound healing processes using excision wound model in rat was also designed. The results revealed complete wound healing (100% contraction) at day 14(10% juice), day 17 (80% juice) and day 18(10% essential oil) compared to 22 days. Histological studies showed that at day 14 complete epithelization, well formed small sized scar tissue and reappearance of cutaneous appendages were evident in wounds painted with 10% essential oil, followed by 80% juice. In vitro study proved a stimulatory effect of plant extracts on human fibroblasts which may explain the speeding of healing process. The healing promoting effect or P. tenuiflorus may be attributed to the high content of calcium (903.16333±0.21); zinc (0.37933±0.05). Essential amino acids (Ala, Leu, Glu, Asp, Asn, Phe and His) seemed also to have a role. On the other hand, thymol was known to have an anti-bacterial effect. Thymol found in this study to be the main component (82.16%) of P. tenuiflorus extracts. © 2010 Asian Network for Scientific Information.

A cytoskeleton-associated protein, TMAP/CKAP2, is involved in the proliferation of human foreskin fibroblasts

Previously, we reported the cloning of a cytoskeleton-associated protein, TMAP/CKAP2, which was up-regulated in primary human gastric cancers. Although TMAP/CKAP2 has been found to be expressed in most cancer cell lines examined, the function of CKAP2 is not known. In this study, we found that TMAP/CKAP2 was not expressed in G0/G1 arrested HFFs, but that it was expressed in actively dividing cells. After initiating the cell cycle, TMAP/CKAP2 levels remained low throughout most of the G1 phase, but gradually increased between late G1 and G2/M. Knockdown of TMAP/CKAP2 reduced pRB phosphorylation and increased p27 expression, and consequently reduced HFF proliferation, whereas constitutive TMAP/CKAP2 expression increased pRB phosphorylation and enhanced proliferation. Our results show that this novel cytoskeleton-associated protein is expressed cell cycle dependently and that it is involved in cell proliferation.

TR1, a new member of the tumor necrosis factor receptor superfamily, induces fibroblast proliferation and inhibits osteoclastogenesis and bone resorption.

A newly identified member of the tumor necrosis factor receptor (TNFR) superfamily shows activities associated with osteoclastogenesis inhibition and fibroblast proliferation. This new member, called TR1, was identified from a search of an expressed sequence tag database, and encodes 401 amino acids with a 21-residue signal sequence. Unlike other members of TNFR, TR1 does not contain a transmembrane domain and is secreted as a 62 kDa glycoprotein. TR1 gene maps to chromosome 8q23-24.1 and its mRNA is abundantly expressed on primary osteoblasts, osteogenic sarcoma cell lines, and primary fibroblasts. The receptors for TR1 were detected on a monocytic cell line (THP-1) and in human fibroblasts. Scatchard analyses indicated two classes of high and medium-high affinity receptors with a kD of approximately 45 and 320 pM, respectively. Recombinant TR1 induced proliferation of human foreskin fibroblasts and potentiated TNF-induced proliferation in these cells. In a coculture system of osteoblasts and bone marrow cells, recombinant TR1 completely inhibited the differentiation of osteoclast-like multinucleated cell formation in the presence of several bone-resorbing factors. TR1 also strongly inhibited bone-resorbing function on dentine slices by mature osteoclasts and decreased 45Ca release in fetal long-bone organ cultures. Anti-TR1 monoclonal antibody promoted the formation of osteoclasts in mouse marrow culture assays. These results indicate that TR1 has broad biological activities in fibroblast growth and in osteoclast differentiation and its functions.

Endothelial cell mitogenesis induced by LPA: Inhibition by thrombospondin-1 and thrombospondin-2

We examined the effects of thrombospondin-1 (TSP1) and thrombospondin-2 (TSP2) on the uptake of tritiated thymidine by bovine aortic endothelial (BAE) cells in response to two growth factors, basic fibroblast growth factor (bFGF) and lysophosphatidic acid (LPA). bFGF and LPA stimulate cell proliferation through distinct receptors that have convergent signaling pathways. The doses of LPA that trigger proliferation of BAE cells, which have not been reported previously, were 1 to 30 μmol/L, as opposed to the 5 to 100 μmol/L concentrations required to stimulate proliferation of human foreskin fibroblasts. Baseline mitogenic activity and activity stimulated by either bFGF or LPA on BAE cells was inhibited by human TSP1 purified from platelets or a recombinant source with a similar dose response. These results demonstrate that the anti-proliferative effect of platelet TSP1 is not caused by contaminants from the stimulated platelet. Recombinant mouse TSP2 inhibited BAE cell proliferation in response to LPA in a dose range similar to that of TSP1. Inasmuch as TSP2 does not activate latent TGFβ1 (Schultz-Cherry et al., J Biol Chem 1995; 270: 7304), these results show that inhibition of angiogenesis by TSPs is not related to control of activation of TGFβ. Together, these studies suggest that structural motifs common to TSP1 and TSP2 inhibit endothelial cell proliferation. Furthermore, TSPs inhibit cell proliferation stimulated by two growth factor receptors that act through distinct signaling pathways.