Progression Of Healing Process Research Articles

Dual antituberculosis drugs-loaded gelatin hydrogel bioimplant for treating spinal tuberculosis

Spinal tuberculosis (TB) represents around 1% of the recorded TB with a high mortality rate due to neurological complications and kyphosis. The current work aimed to develop a bioimplant scaffold to treat spinal TB disease. The scaffold is composed of a biocompatible semi-interpenetrating (semi-IPN) gelatin-based hydrogel incorporating mesoporous silica nanoparticles (MPS-NPs) loaded with rifampicin (RIF) and levofloxacin (LEV) to treat TB. The elastic modulus of the hydrogel was 7.18 ± 0.78 MPa. Minimum inhibitory concentrations (MIC) value against Mycobacterium bovis for LEV-loaded and RIF-loaded MPS-NPs were 6.50 and 1.33 µm/ml, respectively.Sequential release of drugs was observed after 15 days. Loading of the MPS-NPs in the hydrogel matrix governed the amount of released drugs by prolonging the period of release up to 60 days. WST-1 test confirmed the biocompatibility and safety of the developed vertebral hydrogel bioimplant. Histological and immunohistochemistry micrographs showed the progress in healing process with the bioimplant. Besides, loading of LEV and RIF in the implants declined the presence of the giant macrophages clusters as compared to control groups. All the obtained results support the potential use of the developed vertebral hydrogel bioimplant as a scaffold with good mechanical and biocompatible properties along with a good ability to eradicate the TB pathogen.

RADIOGRAPH OF THE USE OF PIG’S BONE AS GRAFT MATERIAL TO FEMUR FRACTURE TREATMENT IN DOGS

Fracture is one of the cases that may occur in pets, especially dogs and cats. The principle to treatment cases of fracture is repositioned and immobilized in the area of ??the fracture. Large bone damage due to trauma can inhibit healing process and cause bone defects, so that graft material is needed to stimulate the healing process and to fill in the missing bone. This research was aimed to study radiographic imaging in the use of pig’s bone as graft material for the treatment of fractures in dogs. Eight male dogs aged 3-4 months were used in this study and were divided into 2 groups randomly. Group I (control) was 2 dogs who their bone diaphysis femur was drilled with a diameter of 1 cm without giving graft material. Group II was 6 dogs who were drilled as Group I and were given a graft material. Monitoring the progress of healing process by rontgent, was conducted at 24 hours, 2nd week, 4th week and 8th week post-surgery. Radiographic analysis showed that there has been a unification and mineralization of bone fragments in the 8th week post-surgery in the group II with bone density already seemed normal.