Productivity Of Cultures Research Articles

The collaborative agglomeration of industries and the realization of the digital economy on the green high-quality development of cities

PurposeThis study explores the role of industrial co-agglomeration and digital economy in influencing the green high-quality development of the Yellow River National Cultural Park to provide countermeasures and suggestions for promoting the whole-area high-quality development.Design/methodology/approachThis study is based on panel data from 56 cities from 2010 to 2022. First, a Super-SBM model is built to evaluate green high-quality development. Secondly, location entropy is used to measure industrial co-agglomeration and the entropy weight method is used to measure the digital economy. Finally, the panel Tobit model is used to analyze the impact of industrial co-agglomeration and digital economy on the green high-quality development of Yellow River National Cultural Park.FindingsThis study found that (1) industrial co-agglomeration has a negative implication in green high-quality development, while the digital economy boosts green high-quality development; (2) industrial co-agglomeration is a less critical dependency on the level of development of the digital economy in influencing green high-quality development, while the facilitating effect of the digital economy is more dependent on industrial co-agglomeration and (3) the trend of slow growth in industrial co-agglomeration and digital economy development, with significant regional differences in green high-quality development.Research limitations/implicationsUndeniably, our study has several limitations. Firstly, as the study area only includes some cities in individual provinces, such as Qinghai, this paper only analyzes at the city level, which does not better reflect the differences between provinces; secondly, this study only adopts one method to determine the digital economy. In the future, other methods can be explored to measure digital economy; finally, in addition to the main role of digital economy and industrial co-agglomeration, other factors may also affect the green high-quality development of YRNCP. Future research should introduce other variables to improve the theoretical framework.Practical implicationsFirst, it provides countermeasures and suggestions for promoting the green high-quality development of YRNCP. Second, it helps to implement the new development concept, cultivate the new quality productivity of culture and the tourism industry and promote the green high-quality development of YRNCP. Third, it provides references to improve the management measures and related policies of the YRNCP more accurately and efficiently. Fourth, it helps to build a new development pattern and has important practical significance in promoting the high-quality development of the whole basin, protecting and inheriting the Yellow River Culture and helping the Chinese-style modernization and development, which are of great practical significance.Social implicationsThe research is carried out from the new perspective of industrial co-agglomeration and digital economy, which provides the theoretical basis and reference for solving the problem of green high-quality development of YRNCP. Second, it broadens the research idea of green high-quality development. Third, it quantitatively analyzes the impact of industrial co-agglomeration and digital economy on the high-quality development of YRNCP, deepening the research on the green high-quality development of YRNCP. Fourth, it helps to enrich and improve the theoretical research related to the national cultural park development and has positive significance in promoting the management and innovation of the cultural industry and the construction of related disciplines.Originality/valueThe paper’s findings illustrate the functional relationship of the digital economy and industrial co-agglomeration with green high-quality development and propose countermeasures to facilitate the high-quality development of the Yellow River National Cultural Park.

Hybrid modeling for in silico optimization of a dynamic perfusion cell culture process.

The bio-pharmaceutical industry heavily relies on mammalian cells for the production of bio-therapeutic proteins. The complexity of implementing and high cost-of-goods of these processes are currently limiting more widespread patient access. This is driving efforts to enhance cell culture productivity and cost reduction. Upstream process intensification (PI), using perfusion approaches in the seed train and/or the main bioreactor, has shown substantial promise to enhance productivity. However, developing optimal process conditions for perfusion-based processes remain challenging due to resource and time constraints. Model-based optimization offers a solution by systematically screening process parameters like temperature, pH, and culture media to find the optimum conditions in silico. To our knowledge, this is the first experimentally validated model to explain the perfusion dynamics under different operating conditions and scales for process optimization. The hybrid model accurately describes Chinese hamster ovary (CHO) cell culture growth dynamics and a neural network model explains the production of mAb, allowing for optimization of media exchange rates. Results from six perfusion runs in Ambr® 250 demonstrated high accuracy, confirming the model's utility. Further, the implementation of dynamic media exchange rate schedule determined through model-based optimization resulted in 50% increase in volumetric productivity. Additionally, two 5 L-scale experiments validated the model's reliable extrapolation capabilities to large bioreactors. This approach could reduce the number of wet lab experiments needed for culture process optimization, offering a promising avenue for improving productivity, cost-of-goods in bio-pharmaceutical manufacturing, in turn improving patient access to pivotal medicine.

Lacticaseibacillus paracsei HY7207 Alleviates Hepatic Steatosis, Inflammation, and Liver Fibrosis in Mice with Non-Alcoholic Fatty Liver Disease.

Non-alcoholic fatty acid disease (NAFLD) is caused by a build-up of fat in the liver, inducing local inflammation and fibrosis. We evaluated the effects of probiotic lactic acid-generating bacteria (LAB) derived from a traditional fermented beverage in a mouse model of NAFLD. The LAB isolated from this traditional Korean beverage were screened using the human hepatic cell line HepG2, and Lactocaseibacillus paracasei HY7207 (HY7207), which was the most effective inhibitor of fat accumulation, was selected for further study. HY7207 showed stable productivity in industrial-scale culture. Whole-genome sequencing of HY7207 revealed that the genome was 2.88 Mbp long, with 46.43% GC contents and 2778 predicted protein-coding DNA sequences (CDSs). HY7207 reduced the expression of lipogenesis and hepatic apoptosis-related genes in HepG2 cells treated with palmitic acid. Furthermore, the administration of 109 CFU/kg/day of HY7207 for 8 weeks to mice fed an NAFLD-inducing diet improved their physiologic and serum biochemical parameters and ameliorated their hepatic steatosis. In addition, HY7207 reduced the hepatic expression of genes important for lipogenesis (Srebp1c, Fasn, C/ebpa, Pparg, and Acaca), inflammation (Tnf, Il1b, and Ccl2), and fibrosis (Col1a1, Tgfb1, and Timp1). Finally, HY7207 affected the expression of the apoptosis-related genes Bax (encoding Bcl2 associated X, an apoptosis regulator) and Bcl2 (encoding B-cell lymphoma protein 2) in the liver. These data suggest that HY7207 consumption ameliorates NAFLD in mice through effects on liver steatosis, inflammation, fibrosis, and hepatic apoptosis. Thus, L. paracasei HY7207 may be suitable for use as a functional food supplement for patients with NAFLD.

ANALISIS BUDAYA DAN PRODUKTIVITAS KERJA PETANI PLASMA PADA PERKEBUNAN KELAPA SAWIT (STUDI KASUS PADA PT. SULTRA PRIMA LESTARI)

This study aims to determine the work culture and work productivity of plasma farmers working at PT Sultra Prima Lestari and the relationship between the two. The population of this study is all plasma farmers who work at PT Sultra Prima Lestari in Andowia District, North Konawe Regency, totaling 120 people divided into 10 farmer groups. Determination of the number of research samples using the Slovin formula with an error rate of 25%, so that the research sample amounted to 54 respondents. This research uses a quantitative approach. The sampling technique used a proportional random sampling technique. Data collection in the field using survey methods, interviews, and documentation using instruments in the form of questionnaires or questionnaires. Variables in this study, namely the work culture of farmers (variable X) and work productivity of farmers (variable Y). The data were analyzed in a quantitative descriptive way using the class interval formula and the Spearman rank correlation formula with the help of SPSS 26 software. The results showed that the work culture of plasma farmers included togetherness, and work intensity in the production of oil palm farming at PT Sultra Perima Lestari was in the high category. The work productivity of plasma farmers includes quality of work, quantity of work, and timeliness of work in the production of oil palm farming at PT Sultra Perima Lestari is in the high category. Work culture with plasma farmers' work productivity has a significant relationship with a value of 0.491 ≥ r table 0.26, meaning it is quite close and the sig value of 0.00 is smaller than the significance level at the α level of 0.05.

The mediating role of workplace incivility on the relationship between organizational culture and employee productivity: a systematic review

The mediating role of workplace incivility on the relationship between organizational culture and employee productivity: a systematic review

Behavior of ponkan mandarin tree canopy grafted onto rangpur lime and flying dragon rootstocks

The Rangpur lime rootstock was the basis of Brazilian citrus growing for many years. However, this rootstock has been gradually replaced due to the occurrence of Citrus Sudden Death. The Flying Dragon rootstock has stood out as one of the options for replacement as it has different characteristics, such as reducing the size of the plant. There is no information in the literature about the behavior of Flying Dragon as a rootstock for Ponkan mandarin. Therefore, the objective of this study was to evaluate the behavior of Ponkan mandarin tree canopy grafted onto Rangpur lime and Flying Dragon rootstocks concerning physiological, agronomic, and fruit quality parameters. In the experiment, productivity, soil moisture, water potential, plant height, fruit diameter, fruit length, fruit mass, fruit pH, soluble solids content, titratable acidity, and ratio were evaluated. The Flying Dragon rootstock was responsible for the decrease in average productivity in the Ponkan mandarin culture. Greater plant heights were obtained when canopies were grafted on Rangpur lime. The soluble solids content of fruits from scions grafted on Flying Dragon was higher, but the degree of uniformity of the soluble solids values obtained from the Rangpur lime tree was higher.

Effects of chronic oral exposure to insecticide teflubenzuron on the midgut of the honey bee Apis mellifera workers: histopathological insights into pesticide toxicity.

The honey bee Apis mellifera plays a significant role as a pollinator of native and cultivated plants, by increasing the productivity of several cultures, preserving the flora, and producing forest seeds. However, bee populations are declining worldwide, including A. mellifera, due to Colony Collapse Disorder, mainly resulting from the constant use of pesticides in the crops. Teflubenzuron is a physiological insecticide that belongs to the benzoylurea group, which inhibits chitin synthesis, the main component of the insect integument classified as safe for non-target insects, including bees. However, its effect on non-target organs of insects remains unknown. The midgut is the main organ of the digestive tract, which works in digestion and absorption and may be exposed to pesticides that contaminate food resources. The present work aimed to verify if the insecticide teflubenzuron is toxic and has histopathological effects on the midgut of A. mellifera adult workers. Workers exposed orally and chronically to the field-realistic concentration of teflubenzuron present 81.54% mortality. The epithelium of the midgut of these bees presents high vacuolization, spherocrystals, cell fragments released to the organ lumen, apocrine secretion, nuclear pyknosis, loss of cell-cell contact, and damage to regenerative cell nests and to the peritrophic matrix. These results indicate that the chitin synthesis-inhibiting insecticide teflubenzuron is toxic to A. mellifera after chronic oral exposure, at realistic field concentration, although it is classified as non-toxic to adult and non-target insects.

Biomass specific perfusion rate as a control lever for the continuous manufacturing of biosimilar monoclonal antibodies from CHO cell cultures.

Continuous manufacturing enables high volumetric productivities of biologics such as monoclonal antibodies. However, it is challenging to maintain both high viable cell densities and productivities at the same time for long culture durations. One of the key controls in a perfusion process is the perfusion rate which determines the nutrient availability and potentially controls the cell metabolism. Cell Specific Perfusion Rate (CSPR) is a feed rate proportional to the viable cell density while Biomass Specific Perfusion Rate (BSPR) is a feed rate proportional to the biomass (cell volume multiply bycell density). In this study, perfusion cultures were run at three BSPRs in the production phase. Low BSPR favored a growth arresting state that led to gradual increase in cell volume, which in turn led to an increase in net perfusion rate proportional to the increase in cell volume. Consequently, at low BSPR, while the cell viability and cell density decreased, high specific productivity of 55 pg per cell per day was achieved. In contrast, the specific productivity was lower in bioreactors operating at a high BSPR. The ability to modulate the cell metabolism by using BSPR was confirmed when the specific productivity increased after lowering the BSPR in one of the bioreactors that was initially operating at a high BSPR. This study demonstrated that BSPR significantly influenced cell growth, metabolism, and productivity in cultures with variable cell volumes.

THE ROLE OF THE LEADER IN THE FORMATION OF CORPORATE CULTURE OF THE ENTERPRISE

Relevance: the role of leadership in shaping the corporate culture of an enterprise is crucial for the success and sustainable development of the organization. Purpose: the article aims to determine the importance of leadership qualities in the process of formation and development of corporate culture of enterprises. Research methods: to achieve this goal, the article uses a methodology that includes analytical review, categorization and integration of data from various sources to better understand the relationship between leadership and corporate culture, which allowed to identify effective approaches to cultivating corporate values. The study found that leaders play a key role in shaping corporate culture through their personal and professional qualities, ability to motivate and unite the team, and ability to implement corporate values in the company's daily operations. Various approaches to management, from strict control to open participation, have a significant impact on the formation of corporate culture, which requires leaders to be flexible in choosing their leadership style based on the needs of the business and its strategic vision. The effectiveness and productivity of corporate culture depend on the ability of leaders to demonstrate diverse qualities that include not only professionalism and personal characteristics, but also social responsibility, value orientations and strategic marketing thinking. Such qualities contribute not only to effective management and team spirit, but also to creating an atmosphere conducive to innovation and longterm development. Corporate culture is a key factor that affects all areas of an organization's life, with people at the center of this process with their relationships, respect and personal growth, which is the basis for creating an effective work environment. Practical significance: the results of the study can be used by managers to formulate an effective strategy for the development of corporate culture.

Metabolic engineering of CHO cells towards cysteine prototrophy and systems analysis of the ensuing phenotype

Chinese hamster ovary (CHO) cells require cysteine for growth and productivity in fed-batch cultures. In intensified processes, supplementation of cysteine at high concentrations is a challenge due to its limited solubility and instability in solution. Methionine can be converted to cysteine (CYS) but key enzymes, cystathionine beta-synthase (Cbs) and cystathionine gamma-lyase (Cth), are not active in CHO cells resulting in accumulation of an intermediate, homocysteine (HCY), in cell culture milieu. In this study, Cbs and Cth were overexpressed in CHO cells to confer cysteine prototrophy, i.e., the ability to grow in a cysteine free environment. These pools (CbCt) needed homocysteine and beta-mercaptoethanol (βME) to grow in CYS-free medium. To increase intracellular homocysteine levels, Gnmt was overexpressed in CbCt pools. The resultant cell pools (GnCbCt), post adaptation in CYS-free medium with decreasing residual HCY and βME levels, were able to proliferate in the HCY-free, βME-free and CYS-free environment. Interestingly, CbCt pools were also able to be adapted to grow in HCY-free and CYS-free conditions, albeit at significantly higher doubling times than GnCbCt cells, but couldn't completely adapt to βME-free conditions. Further, single cell clones derived from the GnCbCt cell pool had a wide range in expression levels of Cbs, Cth and Gnmt and, when cultivated in CYS-free fed-batch conditions, performed similarly to the wild type (WT) cell line cultivated in CYS supplemented fed-batch culture. Intracellular metabolomic analysis showed that HCY and glutathione (GSH) levels were lower in the CbCt pool in CYS-free conditions but were restored closer to WT levels in the GnCbCt cells cultivated in CYS-free conditions. Transcriptomic analysis showed that GnCbCt cells upregulated several genes encoding transporters as well as methionine catabolism and transsulfuration pathway enzymes that support these cells to biosynthesize cysteine effectively. Further, ‘omics analysis suggested CbCt pool was under ferroptotic stress in CYS-free conditions, which, when inhibited, enhanced the growth and viability of these cells in CYS-free conditions.

Culture and Behavior Boost Productivity While Loyalty Lowers It in Indonesia

This study explores the relationships between organizational culture, job loyalty, work behavior, and employee productivity at the SAMSAT Sidoarjo Office, with job satisfaction as a mediating factor. Using a quantitative approach and total sampling, data were collected from 130 employees via questionnaires and analyzed using Smart Partial Least Square (SmartPLS). The results reveal that organizational culture and work behavior positively impact productivity, while job loyalty has a negative effect. All three variables positively influence job satisfaction, which in turn enhances productivity. The findings underscore the importance of fostering a supportive organizational culture and positive work behaviors to boost employee satisfaction and productivity. Highlights: 1. Positive Influence: Culture and behavior boost productivity and satisfaction. 2. Negative Impact: Job loyalty decreases productivity. 3. Mediating Role: Job satisfaction mediates culture, loyalty, behavior impacts on productivity. Keywords: Organizational culture, job loyalty, work behavior, employee productivity, job satisfaction

Use of spectroscopic process analytical technology for rapid quality evaluation during preparation of CHO cell culture media.

Media preparation parameters contribute significantly to media quality, cell culture performance, productivity, and product quality. Establishing proper media preparation procedures is critical for ensuring a robust CHO cell culture process. Process analytical technology (PAT) enables unique ways to quantify assessments and improve media quality. Here, cell culture media were prepared under a wide range of temperatures (40-80°C) and pH (7.6-10.0). Media quality profiles were compared using three real-time PATs: Fourier-transform infrared (FTIR) spectroscopy, Raman spectroscopy, and excitation-emission matrix (EEM) spectroscopy. FTIR and Raman spectroscopies identified shifts in media quality under high preparation temperature (80°C) and at differing preparation pH which negatively impacted monoclonal antibody (mAb) production. In fed-batch processes for production of three different mAbs, viable cell density (VCD) and cell viability were mostly unaffected under all media preparation temperatures, while titer and cell specific productivity of mAb decreased when cultured in basal and feed media prepared at 80°C. High feed preparation pH alone was tolerated but cell growth and productivity profiles deviated from the control condition. Further, charge variants (main, acidic, basic species) and glycosylation (G0F, afucosylation, and high mannose) were examined. Statistically significant differences were observed for one or more of these quality attributes with any shifts in media preparation. In this study, we demonstrated strong associations between media preparation conditions and cell growth, productivity, and product quality. The rapid evaluation of media by PAT implementation enabled more comprehensive understanding of different parameters on media quality and consequential effects on CHO cell culture.

Effect of microalgae (Tetraselmis chui Butcher 1959) on growth and survival of nile tilapia (Oreochromis niloticus Linnaeus 1758) reared in low salinity biofloc system

Addition of microalgae in the biofloc system may improve growth and survival of Nile tilapia. Also, culturing Nile tilapia in saline water biofloc system may be an adaptation for freshwater shortage. In this study, the growth and survival of Nile tilapia cultured in a low salinity biofloc system with added microalgae was investigated. Increasing salinity in the culture system was done by gradually adding sea water into acclimatization container (618 L, v) on weekly basis for 4 weeks until 9 psu salinity was reached. Tilapia were allowed to adapt to salinity change for one week and fed with commercial feed twice a day until satiation. The biofloc experiment was conducted with 3 microalgal density treatments (5, 15 and 25 × 104 cells. mL-1) in triplicates. Average initial weight of experimental fish was 4.82 ± 0.76 g. Tilapia were reared for 40 days in aquaria (35 × 35 × 40 cm, l × w × h, 15 L, v) containing 8 individuals fish in each aquarium. The commercial pellets were delivered twice per day at 5% of tilapia biomass until satiation. T. chui was added to the tilapia culture every week. This study showed that the addition of microalgae did not affect the fish weight gain, specific growth rate and survival (p0.05). However, feed conversion ratio and culture productivity were better in tilapia supplied with weekly addition of T. chui at 15 × 104 cells mL-1 compared to other densities tested.Keywords:SalineBioflocMicroalgae

Medico-biological aspects of physical training of students of higher education institutions

The article analyzes the medical and biological aspects of the physical training of students of higher education institutions. The organizational and methodical principles on which the innovative educational technology of implementing the health-forming orientation of modernized physical education is based are highlighted.
 It was established that the level of physical culture of young people is determined, first of all, by special education in this field, the perceived need to use physical exercises and other types of motor activity for the purpose of strengthening health and comprehensive self-improvement. On the basis of the analysis of a complex of literary sources, the goal of physical education is summarized, which consists in improving the health, education and education of student youth.
 The use of medical and biological aspects for the study of physical culture and health measures, where physical training is considered from the standpoint of the formation of skills of motor activity of the individual and the biological properties of the human body, is substantiated.
 The typological characteristics of the modern system of physical education in higher education institutions of Ukraine are determined; the interaction of medical, biological and cultural aspects of physical culture, which contribute to increasing the productivity of educational and physical culture and health activities, is scientifically substantiated.
 Medical-biological aspects include the study of health problems and the physical condition of people, their use for the study of physical culture and health measures is determined by the fact that physical training is considered from the point of view of the formation of motor activity and biological properties of the body.
 The specificity of the motivational and value component of the medical and biological training of future specialists was investigated on the basis of a questionnaire of higher education applicants.
 It has been proven that more attention should be paid to the promotion of sports and physical culture in the institution of higher education, to fully provide classrooms for classes with modern sports equipment and inventory, to actively include student youth in the process of physical education and mass sports events.

Immunological lectins in shrimp Penaeus vannamei challenged with infectious myonecrosis virus (IMNV) under low-salinity conditions

Lectins are proteins capable of recognizing and binding to glycan in a specific way. In invertebrates, lectins are a crucial group of Pattern Recognition Proteins (PRRs), activating cellular and humoral responses in the innate immune system. The shrimp Penaeus vannamei is the main crustacean cultivated worldwide, however, the productivity of cultures is strongly affected by diseases, mainly viral ones, such as Infectious Myonecrosis (IMN). Thus, we investigated the participation of five lectins (LvAV, LvCTL4, LvCTL5, LvCTLU, and LvLdlrCTL) in IMNV-challenged shrimp. We verified upregulation gene profiles of lectins after IMNV-challenge, especially in hepatopancreas and gills, in addition to an increase in total hemocytes count (THC) after to 12 h post-infection (hpi). The bioinformatics characterization also revealed several sites of post-translational modification (PTM), such as phosphorylation and glycosylation, which possibly influence the action and stabilization of these lectins. We conclude that LvLdlrCTL and LvCTL5 are the lectins with greater participation in the activation of the immune system against IMNV, showing the greatest potential for PTM, higher upregulation levels, and overlapping with the THC and IMNV viral load.

The Role of Work Attitude in Mediating the Influence of Corporate Culture and Management Support on the Work Productivity of PLN Employees

This research investigates the role of work attitude as a mediator in the relationship between corporate culture, management support, and employee work productivity. Corporate culture and management support are key factors influencing employee productivity. However, there has been no research that thoroughly explores how work attitude mediates the influence of both on work productivity. The population that researchers use is employees of PT PLN (Persero) East Bali Customer Service Implementation Unit (UP3). With as many as 119 employees, the sample was determined with the census method; therefore, the whole population made the sample study. Next, data was collected through distributing questionnaires via Google Forms. The existing data collected was furthermore analyzed with SEM-PLS. The study results find That corporate culture positively affects employee work productivity. Management support has a positive role in the work productivity of PT PLN East Bali employees. Work attitude has a positive role in the work productivity of PT PLN East Bali employees. Corporate culture has a positive effect on the work attitude of PT PLN East Bali employees. Management supports a positive role in the work attitude of PT PLN East Bali employees. Work attitude can mediate the influence of corporate culture on the work productivity of PT PLN East Bali employees. Work attitude can mediate the influence of management support on the work productivity of PT PLN East Bali employees. Expected study: This Can be theoretical and practical in developing human resource management literature and guiding practitioners to increase the effectiveness of management strategies in a competitive work environment.

Administering AEC Professionals’ Project Delivery Cross-Culture Productivity: Malaysia vs United Kingdom

Borderless world is transforming construction professionals on how to communicate and work across the globe. Many missed communication and different work culture could result in many variation orders and time wastage in construction. This paper looks to discuss the mixed research methodology results on how to administer architect, engineer and contractor (AEC) professionals’ cross-work culture productivity during building deployment. 34 participants were selected for semi-structured interviews and participatory observations were conducted in two multidisciplinary teams of architect, engineer, and contractor from the United Kingdom (UK) and the Malaysia firms for six months. Then, SimVision® simulations were use as the Cognitive Organizational Theory (COT) protocols based on structural system and project dexterity parameters. Statistics corroborations were then conducted to obtain generalization and reliability. The case study results showed that the UK and the Malaysian team have similarities in operating nature of building deployment and differences in practice and value preferences when delivering a project, whilst model MMsRwB is the ideal for replicating UK’s project productivity efficiency. The findings could guide international construction professionals or teams from developed and developing countries to joint venture in their country successfully especially for Malaysian context. The paper would firstly discuss the literature, secondly describe the mixed method methodology and then present the expected result. The paper recommends future research on formalizing communication system for AEC professionals through integrating Malaysian AEC transdisciplinary global practice in a design studio education program.

Employee Competence, Corporate Culture, and Employee Productivity: An Empirical Review

Employee Competence, Corporate Culture, and Employee Productivity: An Empirical Review

Influence of Effective Irrigation Water Usage on Carrot Root Productivity and Quality Properties in Soilless Culture

Influence of Effective Irrigation Water Usage on Carrot Root Productivity and Quality Properties in Soilless Culture

The Impact Of Principle's Transformative Leadership On Organizational Culture, Commitment, Behavior And Performance Productivity

This study aims to determine the transformation of the principal's leadership style at MTs Palangkaraya City, Central Kalimantan in building organizational culture, performance commitment, performance behavior and employee performance productivity. This study used a quantitative approach with data collection techniques by distributing questionnaires, with a sample of 166 school principals from a total of 210 schools, thus to determine the characteristics of each respondent and variables, this study used descriptive data analysis. Meanwhile, to test the relationship between variables, inferential analysis can be used using the Structural Equation Modeling (SEM) method and the AMOS version 7 approach. The results of this study explain that leadership style transformation is seen as quite effective in dealing with various changes and is believed to be the strongest leadership model (strong leadership), because it can build a healthy organizational culture, performance commitment and performance behavior, so as to increase the productivity of individual performance to be more effective.