Food Products Research Articles

The Allergen Profile of Two Edible Insect Species-Acheta domesticus and Hermetia illucens.

Edible insect proteins are increasingly introduced as an alternative sustainable food source to address the world's need to feed the growing population. Tropomyosin is the main insect allergen; however, additional potential allergens are not well characterized and the impact of extraction procedures on immunological reactivity is unknown. Proteins from different commercial food products derived from cricket (Acheta domesticus) and black soldier fly (BSF) (Hermetia illucens) are extracted using five different extraction buffers. The proteins are analyzed by SDS-PAGE and immunoblotting using allergen-specific antibodies and crustacean allergic patient sera. IgE binding bands are analyzed by mass spectrometry as well as the complete allergen profile of all 30 extracts. Urea-based buffers are most efficient in extracting insect allergens. Shrimp-specific antibody cross-reactivity to tropomyosin from cricket and BSF indicates high sequence and structural similarity between shrimp and insects. Additional unique allergens are identified in both species, including hemocyanin, vitellogenin, HSP20, apolipophorin-III, and chitin-binding protein. Identifying potential allergenic proteins and their isoforms in cricket and BSF requires specific extraction approaches using urea-based methods. While tropomyosin is the most abundant and immunoreactive allergen, seven unique allergens are identified, highlighting the need for insect species-specific allergen detection in food products.

Biocontrol Mechanisms of Three Plant Essential Oils Against Phytophthora infestans Causing Potato Late Blight.

Late blight, caused by the notorious pathogen Phytophthora infestans, poses a significant threat to potato (Solanum tuberosum) crops worldwide, impacting their quality as well as yield. Here, we aimed to investigate the potential use of cinnamaldehyde, carvacrol, and eugenol as control agents against P. infestans and to elucidate their underlying mechanisms of action. To determine the pathogen-inhibiting concentrations of these three plant essential oils (PEOs), a comprehensive evaluation of their effects using gradient dilution, mycelial growth rate, and spore germination methods was carried out. Cinnamaldehyde, carvacrol, and eugenol were capable of significantly inhibiting P. infestans by hindering its mycelial radial growth, zoospore release, and sporangium germination; the median effective inhibitory concentration of the three PEOs was 23.87, 8.66, and 89.65 μl/liter, respectively. Scanning electron microscopy revealed that PEOs caused the irreversible deformation of P. infestans, resulting in hyphal shrinkage, distortion, and breakage. Moreover, propidium iodide staining and extracellular conductivity measurements demonstrated that all three PEOs significantly impaired the integrity and permeability of the pathogen's cell membrane in a time- and dose-dependent manner. In vivo experiments confirmed the dose-dependent efficacy of PEOs in reducing the lesion diameter of potato late blight. Altogether, these findings provide valuable insight into the antifungal mechanisms of PEOs vis-à-vis late blight-causing P. infestans. By utilizing the inherent capabilities of these natural compounds, we could effectively limit the harmful impacts of late blight on potato crops, thereby enhancing agricultural practices and ensuring the resilience of global potato food production.

A Study on Genetically Engineered Foods: Need, Benefits, Risk, and Current Knowledge.

Food requirements have always been a top priority, and with the exponential growth of the human population, there is an increasing need for large quantities of food. Traditional cultivation methods are not able to meet the current demand for food products. One significant challenge is the shortened shelf-life of naturally occurring food items, which directly contributes to food scarcity. Contaminating substances such as weeds and pests play a crucial role in this issue. In response, researchers have introduced genetically engineered (GE) food as a potential solution. These food products are typically created by adding or replacing genes in the DNA of naturally occurring foods. GE foods offer various advantages, including increased quality and quantity of food production, adaptability to various climatic conditions, modification of vitamin and mineral levels, and prolonged shelf life. They address the major concerns of global food scarcity and food security. However, the techniques used in the production of GE foods may not be universally acceptable due to the genetic alteration of animal genes into plants or vice versa. Additionally, their unique nature necessitates further long-term studies. This study delves into the procedures and growth stages of DNA sequencing, covering the benefits, risks, industrial relevance, current knowledge, and future challenges of GE foods. GE foods have the potential to extend the shelf life of food items, alleviate food shortages, and fulfill the current nutritional food demand.

GlobalGAP compliance costs in Ghana’s small-scale pineapple farming sector

ABSTRACT When farmers implement GlobalGAP they incur specific input costs that arise from quality requirements of the technology. However, due to the difficultly in observing and measuring food quality, previous empirical studies seldom analysed the relationship between quality improvements in food production and total costs of production. They assumed that product quality itself was exogenous and hence had no effect on productive efficiency or cost of production. This study estimates the impact of GlobalGAP on costs of production while accounting for fixed cost improvements and quality endogeneity. Data were obtained from GlobalGAP-certified small-scale pineapple farmers in Ghana. The hypothesis that product quality was exogenous was tested and rejected. Consequently, a quality-adjusted translog cost function was used to identify the main contributors to cost increases on small-scale GlobalGAP-certified pineapple farms. The estimated function exhibited economies of size, implying that most small-scale adopters are unable to increase output and benefit from lower average costs. Production costs arising from improvements in quality imposed by GlobalGAP are most sensitive to changes in plantlet price, followed by wages, agrochemical price and expenditure on capital items. Smaller small-scale farmers are much more sensitive to increases in capital expenditure than are larger small-scale farmers. Key policy recommendations include joint ventures to increase nursery capacity and competition in the market for plantlets, scrutiny of mandatory fees impacting the cost of imported labour-saving inputs, facilitating sharing arrangements between smallholders to lower the cost of on-farm infrastructure, and research to identify constraints preventing certified farmers from exploiting size economies.

Risk assessment in production of functional food products by means of RCA method

Risk assessment in production of functional food products by means of RCA method

Non-market food production can contribute to diverse dimensions of food security according to key informants in northern New England

ABSTRACT Recent studies have found that growing or wild-harvesting some of one’s own food is associated with food security in high-income societies. Yet this research has not established causal relationships, and it measures household food security using indicators that assess only access to market food. To disentangle how non-market food production interacts with food security, we interviewed 26 key informants who play central roles in communities of gardeners, hunters, fishers, foragers, and homesteaders in northern New England, U.S.A. These informants indicated that non-market food production relates ambiguously to short-term food access in high-income societies where market food is cheap relative to wages. But non-market production can enhance all other recognized dimensions of food security: availability, adequacy, acceptability, agency, utilization, stability, and sustainability. Causation can run the other way, too: food insecurity was said to increase the likelihood and intensity of engagement in non-market food production. Yet poverty can deprive food-insecure households of the equipment, money, skills, and land access needed for successfully producing their own food. Overall, our informants portrayed non-market food production as a skills-based safety net for reliably feeding oneself from the landscape through personal and societal crises, from the distant past to the climate-change future.

Forecast of sales of selected food products in retail using Fourier series analysis and non-linear regression

Purpose The purpose of this paper is to apply and verify Fourier series analysis in combination with non-linear regression as a tool of forecasting and planning of inputs in the logistics process of a retail chain store. Design/methodology/approach For many popular products, a significant effect of seasonality of sales is expected; therefore, the method of Fourier series was chosen as one of the main forecast calculation techniques. However, the use of this method directly for forecasting sales has a limitation in the form of a complete reconstruction of the shape of the curve from of the given monitored time. Thus, the forecast is based only on the significant harmonic components from the Fourier series analysis that will participate in forecast forming. In addition, to respect the trend of series, the results of Fourier series analysis are combined with the non-linear regression. Findings The results showed that the number of significant harmonic components from the Fourier series analysis is suitable to reflect the future behaviour of the sale in standard market conditions. Forecasting of the sale and accurate purchase planning of goods has a positive effect on reducing the waste of unsold products after their shelf and on increasing of a customer satisfaction. Research limitations/implications This study has an application in a certain period of time (relatively calm behaviour of the food market) and only for a certain region. Therefore, it is not possible to generalize these results as the behaviour of consumers, e.g. within the state. It will also be interesting to monitor and forecast sales of other food items. Practical implications This provides a practical and relatively simple tool for implementing or improving the process of forecasting seasonally dependent products in the food industry. Originality/value This study shows the possibility of forecast that is based on adding the significant harmonic components from the Fourier series analysis to form forecast with the non-linear regression.

Effect of soil additives on biogeochemistry of ultramafic soils—an experimental approach with Brassica napus L

Ultramafic soils are characterized by low productivity due to the deficiency of macroelements and high content of Ni, Cr, and Co. Incorporation of ultramafic soils for agricultural and food production involves the use of fertilizers. Therefore, this study aims to find the soil additive that decreases the metallic elements uptake by plant using Brassica napus as an example. In this study, we evaluate the effect of manure (0.5 g N/kg of soil), humic acids (1 g of Rosahumus/1 dm3 H2O; 44% C), KNO3 (0.13 g K/kg of soil), lime (12.5 g/kg of soil), (NH4)2SO4 (0.15 g N/kg of soil), and Ca(H2PO4)2) (0.07 g P/kg of soil) on the phytoavailability of metallic elements. The effect of soil additives on metallic elements uptake by Brassica napus was studied in a pot experiment executed in triplicates. Statistical analysis was applied to compare the effects of additives in ultramafic soil on plant chemical composition relative to control unfertilized ultramafic soil (one-way ANOVA and Kruskal–Wallis test). The study shows that in almost all treatments, metallic elements content (Ni, Cr, Co, Al, Fe, Mn) is higher in roots compared to the aboveground parts of Brassica napus except for (NH4)2SO4, in which the mechanism of Mn accumulation is opposite. The main differences between the treatments are observed for the buffer properties of soil and the accumulation of specific metals by studied plants. The soils with the addition of lime and manure have the highest buffer properties in acidic conditions (4.9-fold and 2.1-fold increase relative to control soil, respectively), whereas the soil with (NH4)2SO4 has the lowest effect (0.8-fold decrease relative to control soil). Also, the addition of manure increases the biomass of aboveground parts of B. napus (3.4-fold increase) and decreases the accumulation of Ni (0.6-fold decrease) compared to plants cultivated in the control soil. On the contrary, the addition of (NH4)2SO4 noticeably increases the accumulation of Ni, Co, Mn, and Al in aboveground parts of B. napus (3.2-fold, 18.2-fold, 11.2-fold, and 1.6-fold, respectively) compared to plant grown in control soil, whereas the humic acids increase the accumulation of Cr in roots (1.6-fold increase). Therefore, this study shows that manure is a promising fertilizer in agricultural practices in ultramafic soil, whereas (NH4)2SO4 and humic acids must not be used in ultramafic areas.

Impacts of sand and dust storms on food production

Abstract Sand and dust storms (SDS) are common in the world’s drylands, regions that are also critically important for global food production. Agriculture is the most prevalent land use resulting in anthropogenic SDS sources, resulting in impacts on cropland and rangeland, but food production is also affected by impacts from natural SDS sources. This review assesses our knowledge of SDS impacts on all the major types of food production in terrestrial and oceanic environments, impacts that occur in all three phases of the wind erosion system: during particle entrainment, during transport, and on deposition. These effects are short term and long term, direct and indirect. Wind erosion is a major cause of land degradation and there is good evidence to indicate that the deleterious effects of SDS can reduce food production via substantially diminished yields of crops, pastures and livestock. However, it is also clear that soil dust plays an important role in major biogeochemical cycles – especially phosphorus, nitrogen and iron – with implications for the valuable environmental services provided by numerous ecosystems, both terrestrial and marine. Ultimately, these nutrients have particular significance for soil formation, ecosystem productivity and food webs on land and at sea, and hence the provision of food for human societies. Efforts to mitigate the negative impacts of SDS on the sustainability of agriculture should be balanced with an appreciation of the significance of soil dust to the Earth system.

Probabilistic Modelling of the Food Matrix Effects on Curcuminoid’s In Vitro Oral Bioaccessibility

The bioaccessibility of bioactive compounds plays a major role in the nutritional value of foods, but there is a lack of systematic studies assessing the effect of the food matrix on bioaccessibility. Curcuminoids are phytochemicals extracted from Curcuma longa that have captured public attention due to claimed health benefits. The aim of this study is to develop a mathematical model to predict curcuminoid’s bioaccessibility in biscuits and custard based on different fibre type formulations. Bioaccessibilities for curcumin-enriched custards and biscuits were obtained through in vitro digestion, and physicochemical food properties were characterised. A strong correlation between macronutrient concentration and bioaccessibility was observed (p = 0.89) and chosen as a main explanatory variable in a Bayesian hierarchical linear regression model. Additionally, the patterns of food matrix effects on bioaccessibility were not the same in custards as in biscuits; for example, the hemicellulose content had a moderately strong positive correlation to bioaccessibility in biscuits (p = 0.66) which was non-significant in custards (p = 0.12). Using a Bayesian hierarchical approach to model these interactions resulted in an optimisation performance of r2 = 0.97 and a leave-one-out cross-validation score (LOOCV) of r2 = 0.93. This decision-support system could assist the food industry in optimising the formulation of novel food products and enable consumers to make more informed choices.

Social Inclusion and Pest Management: a Route for Improved Food Production and well-being in the Global South.

World trade has facilitated the spread of non-native pest species, presenting new challenges for food production. In the Global South, linking worker social protection programs with invasive pest management can, at the same time, contribute to food security and empower workers. The spotted wing Drosophila (Drosophila suzukii) (SWD) recent invasion in the Global South case study illustrates how science-based policies integrated with agricultural worker-based social organizations can contribute toward economic and environmental sustainability.

Development and Validation of a High-Performance Liquid Chromatography Diode Array Detector Method to Measure Seven Neonicotinoids in Wheat

Neonicotinoids (NEOs), used as insecticides against aphids, whiteflies, lepidopterans, and beetles, have numerous detrimental impacts on human health, including chronic illnesses, cancer, infertility, and birth anomalies. Monitoring the residues in food products is necessary to guarantee public health and ecological balance. The present work validated a new method to measure seven neonicotinoid insecticides (acetamiprid ACT, clothianidin CLT, dinotefuran DNT, imidacloprid IMD, nitenpyram NTP, thiacloprid TCP, and thiamethoxan THT) in wheat. The analytical procedure was based on simple and fast wheat sample cleanup using solid-phase extraction (SPE) to remove interferents and enrich the NEOs, alongside the NEOs’ separation and quantification by reverse-phase chromatography coupled with a diode array detector (DAD). The validation process was validated using the accuracy profile strategy, a straightforward decision tool based on the measure of the total error (bias plus standard deviation) of the method. Our results proved that, in the future, at least 95% of the results obtained with the proposed method would fall within the ±15% acceptance limits. The test’s cost-effectiveness, rapidity, and simplicity suggest its use for determining the levels of acetamiprid, clothianidin, dinotefuran, imidacloprid, nitenpyram, thiacloprid, and thiamethoxam in routine analyses of wheat.

РАЗМЕЩЕНИЕ ПРОИЗВОДСТВА В ИСПРАВИТЕЛЬНЫХ КОЛОНИЯХ РЕГИОНА: ПРИНЦИПЫ И ПРИОРИТЕТЫ

The article considers the placement of industrial and economic activities in institutions of the penal system. As an example, the sectoral structure and territorial location of the production of goods and services of correctional colonies of the GUFSIN of Russia in the Rostov region are given. The main research method is the construction of cartograms (geographical maps with the application of economic and statistical information). Cartograms for the region with the display of penal colonies have been built by industries (sewing, woodworking, metalworking, food production). The analysis using cartograms showed the spatial distribution of industries and its concentration on the territory of the region. The article touches upon the problem of integrating the production and economic activities of institutions of the penal system into the economy of the region. We are talking not only about the economy of the Rostov region, but also about a new integrated structure – the Donbass commonwealth. The authors come to the conclusion that it is necessary to optimize the spatial placement of production facilities of the penal correction system, taking into account the ongoing integration processes in the region.

Heat Stability Assessment of Milk: A Review of Traditional and Innovative Methods

It is important to differentiate milk with different thermostabilities for diverse applications in food products and for the appropriate selection of processing and maintenance of manufacturing facilities. In this review, an overview of the chemical changes in milk subjected to high-temperature heating is given. An emphasis is given to the studies of traditional and state-of-the-art strategies for assessing the milk thermostability, as well as their influencing factors. Traditional subjective and objective techniques have been used extensively in many studies for evaluating thermostability, whereas recent research has been focused on novel approaches with greater objectivity and accuracy, including innovative physical, spectroscopic, and predictive tools.

Climate vulnerability of agroecological and conventional smallholders in Mvomero district, Tanzania: using mixed-methods to uncover local experiences and motivations of farming for the future

Changing rainfall patterns make farmers increasingly vulnerable to crop failure, income loss and food insecurity. Agroecology is proposed to reduce climate vulnerabilities of farmers, as such practices and social movement aim to create more resilient farm and food systems. It is however fairly unknown if, and to what extent, agroecological farmers are better able to cope with climate induced exposures as compared to conventional smallholders. We conducted 194 surveys with agroecological and conventional smallholders to explore the three components of climate vulnerability: exposure, sensitivity and adaptive capacity. We combined this with field observations and interviews, and an analysis of long-term rainfall data. We also followed up the initial survey analysis with additional focus group discussions. Just as climate change occurs incrementally over time, we highlight modest, yet important differences between conventional and agroecological farmers. We find that agroecological farmers are less vulnerable to short-term dry spells, due to a combination of farming practices that improve soil water retention, like mulching and the use of cover crops. However, the use of botanicals might induce new vulnerabilities, as their processing requires additional labor, and sometimes expenditures, and may not protect the crops from pests and diseases. We also find limitations to agroecology in terms of scale, as most farmers are unable to use botanicals on all their farmland. Yet, agroecological farmers process and apply botanicals for health benefits both in production and consumption of foods, and they can occasionally sell their organic farm products for a higher price than conventional famers. With this study, we emphasize that farmers’ reasons to practice agroecology is not just to boost productivity and become more climate resilient, but rather for improving the long-term health of producers, consumers, soils and the environment.

Propagation of Freezing in Supercooled Water-In-Antifreeze-Oil Emulsions.

Water-in-oil (W/O) emulsions, in which water droplets are separated by a continuous oil phase, are frequently used in food and cosmetic products. However, the freezing kinetics of W/O emulsions are not yet well understood. In this study, we find that freezing propagates to individual water droplets that are in direct contact with other frozen droplets. When droplets are not in contact, freezing does not propagate even when the emulsions are cooled to -18 °C. Two measures of the perimeter and the area of the frozen droplets in emulsions are defined to evaluate the propagation velocity of freezing using a simple mathematical model. The velocity is highest (4 × 102 μm s-1) at -18 °C, which is lower than the freezing velocity of individual droplets at the same temperature (1.2 × 103 μm s-1). Water-oil interfaces, or a thin layer of oil between droplets, act as a barrier to propagation of freezing. The dependence of the freezing velocity on the degree of supercooling is consistent with results from a previous study; however, the absolute value of the freezing velocity is smaller by a factor of 102. The propagation velocity also depends on the degree of supercooling but its dependence is different from that of the freezing velocity. Features of freezing of water droplets immersed in antifreeze oil are discussed.

Integrated Population Exposure-Response of Dolutegravir in HIV-1 Supports Bridging of Clinical Response Influenced by Relevant Intrinsic and Extrinsic Patient Characteristics.

Dolutegravir (DTG) is a human immunodeficiency virus type 1 (HIV-1) integrase strand transfer inhibitor indicated in combination with other antiretroviral agents for the treatment of HIV-1 infection in adults and pediatric subjects aged at least 4 weeks. The present work aimed to characterize the viral response based on a pooled analysis of exposure-response (E-R) from five studies in treatment-experienced and integrase-resistant (INI-r) patients infected with HIV-1. Importantly, model-based simulations of the E-R relationships with DTG provided insight into the clinical relevance of known intrinsic (e.g., sub-population with Q148-driven integrase mutation) and extrinsic (food, enzyme inducers, and metal cation-containing products) factors expected to influence the DTG E-R relationship. Model-based post hoc exposure metrics (Cmin and Cavg) were incorporated into a mechanistic population viral dynamic model describing the short-term effect of DTG on log10 HIV-1 RNA viral load over 8 or 10 days. In addition, the impact of DTG in combination with background ARTs on the 24-week HIV RNA response was also assessed using logistic regression. There was good concordance between model-based predictions and observed virologic response on day 10 and week 24. The E-R model-based simulations exploring the potential impact of a higher dose (100 mg b.i.d.) of DTG in subpopulations experiencing exposure changes due to covariates did not show clinically relevant changes in virological response compared with the approved 50 mg b.i.d. clinical dose. Overall, our study confirmed the current recommendation of dolutegravir 50 mg b.i.d. in the integrase inhibitor-resistant (INI-r) population.

Biofortification, metabolomic profiling and quantitative analysis of vitamin B12 enrichment in guava juice via lactic acid fermentation using Levilactobacillus brevis strain KU15152.

Chemical fortification and dose supplementation of vitamin B12 are widely implemented to combat deficiency symptoms. However, in situ, fortification of vitamin B12 in food matrixes can be a promising alternative to chemical fortification. The present study aimed to produce vitamin B12-rich, probiotic guava juice fermented with Levilactobacillus brevis strain KU15152. Pasteurized fresh guava juice was inoculated with 7.2 log CFU mL-1 L. brevis strain KU15152 and incubated for 72 h at 37 °C anaerobically. The antioxidants, total phenolic compounds, vitamin B12 production, sugars, organic acids, pH and viable count were analyzed at 24, 48 and 72 h of incubation. The fermented juice was stored at 4 °C, and the changes in its functional properties were analyzed at 7-day intervals up to 28 days of storage. During fermentation, the bacteria cell count was increased from 7.01 ± 0.06 to 9.76 ± 0.42 log CFU mL-1 after 72 h of fermentation and was decreased to 6.94 ± 0.34 CFU mL-1 during storage at 4 °C after 28 days. The pH, total soluble solids, crude fiber, citric acid and total sugars decreased, while titratable acidity, total protein, antioxidants, phenolic compounds and lactic acid contents increased during fermentation. The fermented guava juice exhibited higher 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS)) radical scavenging activities (85.97% and 75.97%, respectively) at 48 h of fermentation. The concentration of active vitamin B12 in the sample reached 109.5 μg L-1 at 72 h of fermentation. However, this concentration gradually decreased to 70.2 μg L-1 during the storage period. During storage for 28 days at 4 °C, both the fermented and control guava juices exhibited a decline in antioxidant and phenolic compound concentrations. Furthermore, the addition of 20% honey and guava flavor enhanced the organoleptic properties and acceptability of fermented guava juice. The value-added fermented guava juice could be a novel functional food product to combat vitamin B12 deficiency. © 2024 The Author(s). Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Site Specific Nutrient Management (SSNM): Principles, Key Features and its Potential Role in Soil, Crop Ecosystem and Climate Resilience Farming

As the world population expands, farming practices have intensified to meet increased food demand. Modern crop varieties now require higher levels of fertilization compared to traditional organic methods, which are insufficient to meet these new demands. However, inefficient nutrient management and imbalances in field nutrient levels have led to various crop issues. To achieve higher agricultural output and productivity, there has been a notable increase in the application of chemical fertilizers, which unfortunately contributes to soil and water pollution, degradation of soil fertility, and nutrient imbalances. In response, modern technologies are being leveraged to promote sustainable food production and foster balanced agricultural development. Site specific nutrient management (SSNM) aims to empower farmers to adjust fertilizer application dynamically to meet the nutrient requirements of high-yielding crops, bridging the gap between natural nutrient sources like soil, crop residues, manure, and irrigation water. It is based on the principles of 4Rs: right product, right dose, right time and right place. Modern technologies like Optical sensors, Crop Manager, Remote Sensing, Nutrient Expert®, fertility mapping, Active Canopy Sensors, Variable rate technologies etc. are used to enhance the potential of SSNM to higher level. The focus is on optimizing nutrient application timing and rates to maximize crop yield and nutrient efficiency, thereby enhancing the economic value of harvest per unit of fertilizer used, rather than simply increasing or decreasing fertilizer use.

A comprehensive Review of Food Dehydration and Its Application in Aquatic Food Products

Dehydrated foods are the most stable food in the food preservation realm. Food dehydration is preferred as the most effective and intricate process within the realm of food processing. Employing methods such as hot air/heated surfaces, this method reduces the water content from various food items, consequently reducing their water activity and make the food item more stable. The primary objective is to hinder microbial growth and enzyme activity, thereby extending the shelf-life. Nevertheless, it is crucial to acknowledge that the dehydration process can potentially compromise both the quality and nutrition. This necessitates a thoughtful approach in the design and operation of dehydration equipment. Various dehydration methods have been developed based on the nature of food and to attain the desired physico-chemical, nutritional, and sensory alterations.