Sausage Processing Research Articles

Transfer Teknologi Pengolahan Sosis Ikan Thok Bagi Kelompok Masyarakat Gampong Neuheun, Kecamatan Mesjid Raya, Kabupaten Aceh Besar

The technology transfer program for processing Thok fish sausages has been implemented for the fisherwomen in Gampong Neuheun. Based on interviews, the main issues faced are the lack of skills in using materials, tools, and technology for processing Thok fish products. Additionally, the absence of a coordinating business entity for fish processing reduces community motivation. The aim of this activity is to provide Thok fish sausage processing technology to the fisherwomen in Gampong Neuheun. The implementation methods include surveys, socialization, and training, with evaluations through pre-tests and post-tests. Results show a 90% increase in skills and knowledge, as well as an 85% increase in motivation. This training facilitates the management of fish catches during the harvest season.

Prevalence and Characterization of Salmonella during Pork Sausage Manufacturing.

Pork carcasses and meat may harbor Salmonella and may contaminate other products during harvest and fabrication. Sources of contamination include manure on hides, environmental contamination, ingredients from external sources, and lymph nodes. Swine lymph nodes are often incorporated into ground meat, as their anatomical location makes removal labor prohibitive. A sausage processing plant in the midwestern United States was sampled monthly (except for December) from May 2021 to April 2022 to enumerate Salmonella and Enterobacteriaceae (EB) throughout the sausage manufacturing process to determine high-risk stages and efficiency of existing in-plant interventions. Salmonella serotypes and antimicrobial susceptibility were evaluated on a subset of isolates recovered at the end phases of sausage production. In each collection, samples were taken from the carcasses of eight sows through 11 stages of sausage manufacturing. A total of 830 samples were cultured. Thirty-four Salmonella were isolated from the final three production stages; of these, there were eleven serotypes. Three isolates displayed resistance to ampicillin, whereas the remainder of the isolates were pan-susceptible to the antimicrobials tested. Salmonella and EB were significantly reduced (p < 0.001) by acid washes at different stages of production, and the results point to the beneficial effects of interventions to lessen Salmonella concentrations in retail products.

Catfish (Pangasius sp.) Sausage Processing on Physical-Chemical and Microstructure Characters

Catfish (Pangasius sp.) Sausage Processing on Physical-Chemical and Microstructure Characters

Impact of Micronized Salt in Reducing the Sodium Content in Fresh Sausages

This study aimed to investigate the incorporation of micronized salt (MS) to reduce sodium content in fresh sausages while preserving technological, chemical, textural, and sensory characteristics. Four treatments were prepared: control (C) with 2.0% regular salt; M2.0% with 2.0% micronized salt; M1.5% with 1.5% micronized salt; and M1.0% with 1.0% micronized salt, containing 1004, 1133, 860, and 525 mg of sodium/100 g of product, respectively. To characterize the samples, analyses of sodium content, cooking loss, relative myoglobin content, and instrumental color were carried out. The sensory analysis was performed using the Temporal-Check-All-That-Apply (TCATA) method. Half of the micronized salt treatment was mixed with the fat during the processing of the fresh sausages. It was possible to achieve a 50% reduction in sodium (M1.0%) in the fresh sausages without negative effects on most technological, chemical, and textural parameters, which did not differ from the control treatment (C). Conversely, “chewiness” decreased in M2.0% compared to the control (C) due to mixing micronized salt with the fat. The sodium reduction did not impact the temporal sensory profile and overall liking. Therefore, using micronized salt in fresh sausages reduces sodium content without affecting sensory traits and product stability.

The effect of addition of biostarter “bekasam” on the quality and characteristics of fermented catfish (Pangasius sp.) sausage

Fermented fish sausage is one of the processed fish meat products that utilizes the role of Lactic Acid Bacterias (LABs). To accelerate the fermentation process of sausages, it is necessary to add a starter culture in the form of a fermented fish substrate (bekasam) which contains a lot of lactic acid bacterias. This research was conducted using an experimental method and arranged using a Randomized Block Design (RBD). The treatments applied were differences in the concentration of bekasam added, as much as 0%, 2%, 4%, and 6% of the weight of the fish meat, then fermented at room temperature for 6 days. The results showed that the treatment of adding bekasam substrate with a concentration of 4-6% produced the best fermented catfish sausage, which was mostly preferred by panelists. The best fermented sausages produced was characterized with attractive appearance, uniform, consistent, and bright, smell fermented product aroma, a spicy but no fishy aroma, a delicious taste, quite sour as fermented tasted, with quite soft and compact texture. The fermented sausages have pH value 5.00, Aw value 0.77, total acid content 0.66%, TVB-N value 12.31 mgN/100 gs and total LABs obtained 6.9 x 106 cfu/g.

Pengaruh Subtitusi Tepung Jagung Dalam Pembuatan Sosis Ikan Bandeng Dengan Penambahan Bubuk Daun Jeruk Purut

Sausages made from fish are a processed seafood product created from fish paste (surimi), mixed with various seasonings, and then packaged using goat intestines or a similar material known as casing. Essentially, any type of fish, whether from freshwater or seawater, can be processed into sausage form. However, typically, the fish used as raw material for sausage processing consists of non-economic bycatch varieties. This research is designed using a Completely Randomized Design with one factor, which is the concentration of Kaffir lime leaf powder. The study includes one control and three treatments, with parameters such as Total Plate Count and Antioxidant testing. Based on the results, the Total Plate Count for the 9% concentration is 1700 x 102 CFU/g, and the highest antioxidant content in milkfish sausages with the addition of Kaffir lime leaf powder at 9% is 44.91 ppm

Chemical and Organoleptic Quality of Beef Sausage by Substitution of Tapioca with Kepok Banana (Musa paradisiaca formatypica) Flour

One of the beef processing is sausage, which is well known in the community. At this time, the need for fast food (ready-to-cook) is increasing. The main ingredient used to produce sausages for stuffing is tapioca flour. The increasing price of tapioca flour is due to the high demand for its use in processed foods, so it is necessary to find alternative fillers that are more affordable. Kepok banana flour (Musa paradisiaca formatypica) has a starch content that is relatively similar to tapioca, so it can be used as an alternative raw material. This study aimed to determine the chemical and organoleptic quality of beef sausage made with Kepok banana flour. This study used a completely randomized design (CRD) consisting of 5 treatments, namely X0 = (control) 0% Kepok banana flour + 20% tapioca; X1 = 5% Kepok banana flour + 15% tapioca; X2 = 10% Kepok banana flour + 10% tapioca; X3 = 15% Kepok banana flour + 5% tapioca; X4 = 20% Kepok banana flour + 0% tapioca, with each treatment consisting of 4 replicates. The variables observed were protein content, moisture content, crude fibre, fat content, ash content and organoleptic of beef sausage. The results showed that the addition of Kepok banana flour in beef sausage processing had a significant effect (P&lt;0.05) on protein content, fat content, moisture content, crude fibre, color, flavour, and aroma, but no significant effect (P&gt;0.05) on ash content and texture of beef sausage. It is concluded that beef sausage made with kepok banana flour as a substitute for tapioca flour produces sausage with better chemical and organoleptic properties than beef sausage made with tapioca flour only. Kepok banana flour can be used as a filler in the production of beef sausage.

Influence of propionic acid microorganisms on the quality indicators of the produced sausage

The article considered the effect of probiotic bacteria on the quality of meat for semi-smoked sausages. The biochemical activity of propionic acid bacteria was studied at the stage of the production process of semi-smoked sausages after the treatment of raw materials with starter microorganisms. We also studied the effect of salt, and sodium nitrite on the development of propionic acid bacteria in raw beef. The results of the technology of production of semi-smoked sausages treated with propionic acid bacteria in the amount of 0.1%, and the quality characteristics of the finished sausage products are presented. On the basis of experimental studies optimal technological parameters of salting and precipitation in the production of fermented semi-smoked sausages were selected. The use of liquid propionic acid bacteria (2 strains of propionic acid bacteria Propionibacterium shermani) in the amount of 0.1% will give a decrease in the production time of half-smoked sausages by two times. As a result of the research it was proved that the introduction of 2 different strains of propionic acid bacteria concentrate into raw meat accelerates biochemical changes in the process of salting and provides functional and technological properties. Also, the results of the study showed that propionic acid bacteria used in the technology of semi-smoked sausages are resistant to the amount of food salts and sodium nitrite used. As a result of research, a better way of using liquid propionate microorganism in the production of semi-smoked fermented sausages was adopted.

Effect of selected agents for ochratoxin A biocontrol on the colour, texture and volatile profile of dry-cured fermented sausages.

Traditional dry-cured fermented sausages favour the growth of an autochthonous microbial population, which plays an important role in their sensory aspects. However, some moulds can produce mycotoxins such as ochratoxin A (OTA). The biocontrol agents (BCAs) Debaryomyces hansenii FHSCC 253H and Staphylococcus xylosus FHSCC Sx8 have been demonstrated to reduce OTA production in dry-cured meat products, but their influence in the sensory characteristics of sausages has to be tested. The aim of this study was to evaluate the effect of these BCAs on the colour, texture and volatile profile of dry-cured fermented sausages. D. hansenii caused few differences in the tested parameters with respect to the control batch. S. xylosus modified the texture and colour, although the values found were within the range expected for dry-cured fermented sausages 'salchichón'. Additionally, the volatile profile revealed the potential antioxidant effect of both BCAs and their ability to produce compounds associated with the ripened aroma that could increase product acceptability. The results indicate that there were no inconveniences in implementing both BCAs during the processing of dry-cured fermented sausages 'salchichón'. Moreover, D. hansenii FHSCC 253H could improve the volatile profile of this product. © 2023 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Sustainable phase change materials (PCMs): waste fat from cooking pork meat confined in polypropylene fibrous mat from waste surgical mask and porous bio-silica

In this article, waste fat deriving from the cooking process of pork sausages has been studied as a sustainable phase change material (PCM). The waste fat is composed of saturated and unsaturated fatty acids and presents melting point and melting enthalpy values of 32 °C and 20 J/g respectively. The BioPCM was confined in two different hosts, namely (i) biosilica (diatomite) and (ii) polypropylene non-woven mat coming from surgical mask filter waste. Both hosts are effective in entrapping high quantity of BioPCM (i.e. 65-75%) maintaining its physical and thermal properties. A simplified analytical model has been developed to analyze the use of the BioPCM in building passive cooling application simulating the thermal behavior of a building wall containing the BioPCM confined in the hosts. A transmitted thermal power per unit area of 10.2 W/m2 was obtained. This value is 37% less of transmitted thermal power calculated for the wall without the BioPCM (16.2 W/m2), confirming that waste cooking fat can be an effective eco-sustainable solution in a good practice of circular economy.

Inactivation of Toxoplasma gondii in dry sausage and processed pork, and quantification of the pathogen in pig tissues prior to production

Toxoplasma gondii is an important zoonotic foodborne parasite. Meat of infected animals appears to be a major source of infection in Europe. Pork is the most consumed meat in France, with dry sausages well represented. The risk of transmission via consumption of processed pork products is largely unknown, mainly since processing will affect viability but may not entirely inactivate all T. gondii parasites.We investigated the presence and concentration of T. gondii DNA in the shoulder, breast, ham, and heart of pigs orally inoculated with 1000 oocysts (n = 3) or tissue cysts (n = 3) and naturally infected pigs (n = 2), by means of magnetic capture qPCR (MC-qPCR). Muscle tissues of experimentally infected pigs were further used to evaluate the impact of manufacturing processes of dry sausages, including different concentrations of nitrates (0, 60, 120, 200 ppm), nitrites (0, 60, 120 ppm), and NaCl (0, 20, 26 g/kg), ripening (2 days at 16–24 °C) and drying (up to 30 days at 13 °C), by a combination of mouse bioassay, qPCR and MC-qPCR.DNA of T. gondii was detected in all eight pigs, including in 41.7% (10/24) of muscle samples (shoulder, breast and ham) and 87.5% (7/8) of hearts by MC-qPCR. The number of parasites per gram of tissue was estimated to be the lowest in the hams (arithmetic mean (M) = 1, standard deviation (SD) = 2) and the highest in the hearts (M = 147, SD = 233). However, the T. gondii burden estimates varied on the individual animal level, the tissue tested and the parasitic stage used for the experimental infection (oocysts or tissue cysts). Of dry sausages and processed pork, 94.4% (51/54) were positive for T. gondii by MC-qPCR or qPCR, with the mean T. gondii burden estimate equivalent to 31 parasites per gram (SD = 93). Only the untreated processed pork sample collected on the day of production was positive by mouse bioassay.The results suggest an uneven distribution of T. gondii in the tissues examined, and possibly an absence or a concentration below the detection limit in some of them. Moreover, the processing of dry sausages and processed pork with NaCl, nitrates, and nitrites has an impact on the viability of T. gondii from the first day of production. Results are valuable input for future risk assessments aiming to estimate the relative contribution of different sources of T. gondii human infections.

Investigation on the Contents of Nε-carboxymethyllysine, Nε-carboxyethyllysine, and N-nitrosamines in Commercial Sausages on the Chinese Market

Sausages are among the most popular meat products worldwide. However, some harmful products, such as advanced glycation end-products (AGEs) and N-nitrosamines (NAs), can be formed simultaneously during sausage processing. In this study, the contents of AGEs, NAs, α-dicarbonyls and the proximate composition were investigated in two kinds of commercial sausages (fermented sausages and cooked sausages) in the Chinese market. The correlations among them were further analyzed. The results showed that the fermented and cooked sausages had different in protein/fat contents and pH/thiobarbituric acid reactive substance values due to their different processing technologies and added ingredients. The Nε-carboxymethyllysine (CML) and Nε-carboxyethyllysine (CEL) concentrations varied from 3.67 to 46.11 mg/kg and from 5.89 to 52.32 mg/kg, respectively, and the NAs concentrations ranged from 1.35 to 15.88 µg/kg. The contents of some hazardous compounds, such as CML, N-nitrosodimethylamine, and N-nitrosopiperidine, were observed to be higher in the fermented sausages than in the cooked sausages. Moreover, levels of NAs in some sausage samples exceeded the limit of 10 µg/kg issued by the United States Department of Agriculture, suggesting that particular attention should be paid to mitigating NAs, especially in fermented sausages. The correlation analysis suggested that the levels of AGEs and NAs were not significantly correlated in both kinds of sausages.

Application of Meat Post-Harvest Technology By The Housewife Group In Kendari City

Community service aims to provide a comprehensive understanding for the housewife group in Gunungjati Sub-District around Kendari City Market regarding the management of healthy food and livestock products. This activity is expected to provide knowledge and skills related to fresh meat and its processing to obtain food diversification, increase nutritional value, longer shelf life, and added economic value. The method used was training and direct demonstration related to fresh, preserved, and rotten meat as well as the processing of nuggets and sausage. Various meats can be used to make nuggets and sausages such as chicken, beef and buffalo meat. Training materials were related to the importance of consuming fresh meat, the importance of choosing uncontaminated meat with preservatives, and processed livestock products in the form of nuggets and sausages with benefits for human health and can be a source of family income. This activity has a positive impact to make people creative with meat-based ingredients and motivates them to create a business field. The types of meat that have been used for the development of sausage and nugget products are beef, rejected chicken meat and broiler chicken meat

Antibacterial Activity of Selected Essential Oils against Foodborne Pathogens and Their Application in Fresh Turkey Sausages.

Essential oils (EOs) isolated from different plant materials, namely Origanum majorana L., Satureja hortensis L., and Satureja montana L. (OMEO, SHEO, and SMEO, respectively), were used in fresh turkey sausage processing. The chemical composition and in vitro antimicrobial potential of selected EOs and their mixture were determined. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against foodborne pathogens (Escherichia coli, Salmonella Enteritidis, and Listeria monocytogenes) ranged in the interval of 0.44-7.1 µL/mL. Fresh turkey sausages were produced with EOs addition and marked as follows: TOMEO-0.150 µL/g OMEO; TSHEO-0.150 µL/g SHEO; TSMEO-0.150 µL/g SMEO; TEOM-0.050 µL/g OMEO, 0.050 µL/g SHEO and 0.050 µL/g SMEO, and control (C) (without EOs). Microbiological profile and biogenic amines content in fresh turkey sausages were recorded during storage. The selected EOs and their mixture efficiently reduced bacterial growth and biogenic amines formation and accumulation. The lowest Enterobacteriaceae count and total biogenic amine (BA) concentration were determined through treatment TSHEO. The results of this study show that selected EOs could be useful in fresh turkey sausage processing in order to improve safety and shelf-life.

Impact of Nitrite Supplementation on Bioactive Peptides during Sausage Processing.

The goal of this investigation was to examine the impact of nitrite supplementation on the concentration, antioxidant properties, and species of antioxidant peptides in fermented sausages. The polypeptide concentration in nitrite-supplemented sausages was markedly elevated during sausage processing compared to the blank control (p &lt; 0.05). Moreover, nitrite supplementation in fermented sausages markedly enhanced the DPPH, as well as the ABTS, hydroxyl radical, and superoxide anion free radical scavenging abilities (FRSA) of polypeptides (p &lt; 0.05). The ferrous ion chelating ability was also significantly enhanced (p &lt; 0.05). Based on the liquid chromatograph-mass spectrometer (LC-MS) analysis of the sausage, LPGGGHGDL, TKYRVP, FLKMN, SAGNPN, GLAGA, LPGGGT, DLEE, GKFNV, GLAGA, AEEEYPDL, HCNKKYRSEM, TSNRYHSYPWG, and other polypeptides exhibited antioxidant properties. Moreover, the number of species of antioxidant polypeptides in the nitrite-supplemented sausage was greater in comparison to the controls. Based on this evidence, it may be concluded that nitrite supplementation positively modulated antioxidant polypeptide formation in fermented sausages, thereby providing strong evidence that nitrite supplementation significantly enhances sausage quality.

Advanced Glycation End Products and Nitrosamines in Sausages Influenced by Processing Parameters, Food Additives and Fat during Thermal Processing.

Advanced glycation end products (AGEs) and nitrosamines (NAs) in sausage are associated with pathogenic and carcinogenic risks. However, the multiple reaction parameters affecting the production of AGEs and NAs during sausage processing remain unclear. This experiment evaluated the effects of processing parameters, food additives and fat ratios on the formation of AGEs and NAs in sausages. The results showed a 2-3-fold increase in Nε-(carboxymethyl)lysine (CML) and Nε-(carboxyethyl)lysine (CEL) when the sausage processing temperature was increased from 90 °C to 130 °C, and N-nitrosodimethylamine (NDEA) increased from 3.68 ng/g to 6.41 ng/g. The addition of salt inhibited the formation of AGEs and NAs, and the inhibitory ability of 2 g/100 g of salt was 63.6% for CML and 36.5% for N-nitrosodimethylamine (NDMA). The addition of 10 mg/kg nitrite to sausages reduced CML formation by 43.9%, however, nitrite had a significant contribution to the formation of NAs. The addition of fat only slightly contributed to the production of CML. In addition, the relationship between α-dicarbonyl compounds and the formation of AGEs was investigated by measuring the changes in α-dicarbonyl compounds in sausages. The results showed two trends of AGEs and α-dicarbonyl compounds: AGEs increased with the increase in α-dicarbonyl compounds and AGE level increased but α-dicarbonyl compound level decreased.

Influence of surface properties on the adhesion of bacteria onto different casings

Bacteria adhere to the surfaces of sausage casing and form biofilms, which causes food spoilage and quality deterioration. However, bacterial adhesion to the casing surfaces has not received enough attention and has not been extensively studied. In this study, the effect of the physicochemical properties of casing surfaces on bacterial initial adhesion were investigated with Leuconostoc mesenteroides as model bacteria. The adhesion of Leuconostoc mesenteroides onto 5 types of casings were systematically investigated, including animal casings, collagen casings, cellulose casings, fiber casings, and nylon casings, which are the most frequently encountered casings in sausage processing. It was found that the number of viable cells on the casings following the trend as: animal casings > collagen casings > fiber casings > cellulose casings > nylon casings after 4 h of incubation time. This phenomenon might be due to the different physicochemical properties of the different casings. Therefore, physicochemical factors, including zeta potential, hydrophobicity and roughness of casings, zeta potential and hydrophobicity of Leuconostoc mesenteroides, were further characterized. In terms of hydrophobic interactions, the results showed that the number of bacteria attached to the casings did not conform to the trend of hydrophobic interaction. In terms of electrostatic interactions, the results showed that the number of bacteria attached to the casings did not conform to the trend of hydrophobic interaction. The casings with different surface roughnesses in a range of 1.67–20.83 μm, the variation of bacterial adhesion quantity was in good agreement with the variation trend of casing roughness, the result showed that the surface roughness was the key factor dominating the bacterial adhesion rate compared with the surface hydrophobicity and zeta potential. The results give new insights to explore the mechanism of bacterial adhesion on casings and prevent sausage spoilage.

The Effect of Using Secang (Caesalpinia secang Linn) Infusion on the Organoleptic Quality of Chicken Sausage

This study aims to determine the effect of using Secang infusion (Caesalpinia secang Linn) on the organoleptic quality of chicken sausages and determine the optimal level of adding Secang infusion on the organoleptic quality of chicken sausages. This research was carried out in 30 days at the Laboratory of the Faculty of Animal Husbandry, University of Jambi. This study used a randomized block design (RBD) which consisted of 4 treatments and 30 replications (panelists). The concentration of Secang infusion used was 0%, 5%, 10%, and 15%. The variables observed in this study were the value of organoleptic quality, which included color, aroma, taste, texture, and elasticity. The data obtained were analyzed by analysis of variance (ANOVA). If it had a significant effect, it continued with Duncan's test. The results showed that the use of Secang infusion on chicken sausage had a very significant effect (P&lt;0.01) on color but had no effect (P&gt;0.05) on aroma, taste, texture, and elasticity. The higher the concentration of Secang infusion, the more preferred the sausage color will be. It can be concluded that the processing of chicken sausage with the addition of Secang infusion at 10% and 15% level produced the preferred color of the panelist's preference level compared to the 0% and 5% levels. Still, the addition of Secang in the processing of chicken sausage did not make a difference to the level of preference for aroma, taste, texture, and suppleness.

Effects of sodium chloride (NaCl) partial substitution by potassium chloride (KCl) in combination with high pressure on sensory and chemical properties of beef sausage during cold storage at 4°C

ABSTRACT The objectives of this study were to evaluate the effects of partial substitution of 25% and 50% NaCl with KCl coupled with high pressure processing (100 MPa for 5 min at 25°C), on sensory and chemical qualities of beef sausage for 28 days’ storage at 4°C. Three sausage formulations were produced with different concentrations of NaCl (100%, 75% and 50%) and KCl (0%, 25% and 50% respectively). High pressure application reduced the total NaCl content and improved the sensory acceptance for taste and flavor compared to control beef sausages. The addition of KCl showed the lowest values for Na+ content; the highest scores for all sensory parameters, improved hardness and chewiness, with results not statistically different from the results obtained by combining HPP and KCl. Therefore, the use of HPP in combination with KCl was considered a technological and sensorial viable alternative to produce low sodium beef sausages.

Antioxidative effect of dry distilled rose petals extract in traditional Bulgarian dry fermented sausages with reduced nitrate content

The aim of study was to explore the dry distilled rose petals extract (DDRPE) addition to Bulgarian lukanka type dry fermented sausages with half-reduced nitrate content. There was studied the antioxidative effect of DDRPE in lukanka filling mass on color characteristics, pH, acid value (AV), peroxide value (POV), TBARS and sensory characteristics. Four samples: C100 - 0.500 g/kg nitrate without DDRPE, C50 - 0.250 g/kg nitrate without DDRPE, R1 - 0.250 g/kg nitrate and 1.140 g/kg DDRPE and R2 - 0.250 g/kg nitrate and 2.280 g/kg DDRPE were examined dynamically on the 1-st, the 10-th and 18-th d during the sausage processing. The color brightness (L*) at the end of the experiment was the highest in C100 and R1 while the redness (a*) and yellowness (b*) values were higher in R1 samples only. The pH was the lowest in R2 and R1. An increase in AV during the studied period was evidence for an outgoing lipolysis in all samples. In comparison to using 2.280 g/kg DDRPE (R2), the addition of 1.140 g/kg DDRPE (R1) demonstrates a better inhibitory effect on lipid peroxidation (POV, TBARS) of traditional Bulgarian dry fermented sausages. C100 had the highest sensory scores followed by R2.