Potential toxicity of treated effluents of selected natural rubber processing industries was evaluated by integrating physicochemical analysis with Daphnia magna and Poecilia reticulata bioassays as ecotoxicity tools. Further, the efficacy of the constructed wetland treatments practiced by the industries for reducing the ecotoxicity of the final effluents reaching the receiving water course was assessed. Even after passing through the constructed wetlands, some of the measured physicochemical parameters of the final effluents did not comply with the stipulated rubber processing effluent regulatory limits. Acute toxicity data of treated effluents demonstrated greater susceptibility of D. magna compared to P. reticulata. Erythrocytic abnormality tests with P. reticulata revealed that rubber industry effluents contained cytogenotoxic contaminations which had not been completely eliminated by the treatment processes. Wetland treatment technique was not effective in reducing the cytogenotoxic effects of final effluents reaching the receiving water course. The use of ecotoxicity tools for optimization of rubber industry effluent treatment processes would help to reduce potential toxic/cytogenotoxic effects of effluent receiving waterbodies considering sustainable development goals focusing on ecosystem safety.