Aging Process Research Articles

Identification of the degree of aging and adsorption behaviors of the naturally aged microplastics

Microplastics (MPs) inevitably experienced various aging processes in nature may exhibit varied and complex interfacial interactions with adjacent species. Therefore, clarifying the possible interfacial interactions between naturally aged MPs and organic pollutants is of great significance to assess the actual behaviors of MPs in the environment. Here several plastic packaging materials after use were employed as the raw materials and representatives of naturally aged MPs, the alteration of surface characteristics, especially the degree of aging and the adsorption properties of MPs for anionic and cationic dyes were investigated. The types and the degree of aging of MPs were identified, and the variation of oxygen-containing functional groups (carbonyl, hydroxyl, and ester groups), the hydrophilicity and surface charge character were characterized. The fitting results of kinetics and isotherm models indicated that the adsorption was mainly multi-layer on heterogeneous surfaces, with hydrogen bonding, electrostatic attraction, polar interaction, and hydrophobic partitioning possibly involving. The hydrogen bond interaction was further confirmed by FTIR spectra. The increased temperature promoted the adsorption of cationic dyes on MPs, and the increased salinity of the solution enhanced the uptake of most of the tested dyes by MPs. This research deepened the understanding on the aging degree of MPs and their interfacial interactions with hydrophilic pollutants, and provided vital information for MPs as pollutant carriers.

Senolytics Enhance the Longevity of Caenorhabditis elegans by Altering Betaine Metabolism.

Aging triggers physiological changes in organisms that are tightly linked to metabolic changes. Senolytics targeting many fundamental aging processes are currently being developed. However, the host metabolic response to natural senescence and the molecular mechanism underlying the antiaging benefits of senolytics remain poorly understood. In this study, we investigated metabolic changes during natural senescence based on the Caenorhabditis elegans model and pinpointed potential biomarkers linked to the benefits of senolytics. These results suggest that age-dependent metabolic changes during natural aging occur in C elegans. Betaine was identified as a crucial metabolite in the natural aging process. We explored the metabolic effects of aging interventions by administering 3 antiaging drugs-metformin, quercetin, and minocycline-to nematodes. Notably, betaine expression significantly increased under the 3 antiaging drug treatments. Our findings demonstrated that betaine supplementation extends lifespan, primarily through pathways associated with the forkhead box transcription factor (FoxO) signaling pathway, the p38-mitogen-activated protein kinase (MAPK) signaling pathway, autophagy, the longevity regulating pathway, and the target of rapamycin (mTOR) signaling pathway. In addition, autophagy and free radicals are altered in betaine-treated nematodes. Overall, we found that betaine is a critical metabolite during natural aging and that senolytics extend the lifespan of nematodes by increasing betaine levels and promoting autophagy and antioxidant activity. This finding suggests that betaine could be a novel therapeutic target for promoting longevity.

Anecdotal Evidence Elucidates the Aging Process.

Our group at Yuvan Research has conducted several experiments in recent years that have demonstrated the reversibility of aging through the use of a young plasma fraction, following a historical line of research that began with heterochronic parabiosis. However, a one-of-a-kind discovery, in the form of anecdotal evidence, has recently clarified many doubts about the nature of aging and rejuvenation, and the conclusions that can be drawn from this discovery allow us to form a relatively clear picture of the mechanics of the aging and rejuvenation processes.

The Effect of Circulating Exosomes Obtained from Young and Old Individuals on the Aging related hTERT and P16 Expression in Hematopoietic Stem Cells

Introduction: Functional reduction of telomeres can induce DNA damage response through cell cycle checkpoints and contribute to the senescence of stem cells. The effect of exosomes on the aging and rejuvenation of hematopoietic stem cells (HSCs) is not well known. Therefore, the present study is designed to examine the impact of plasma exosomes derived from young and old individuals on hTERT and P16 expression involved in the cellular aging process. Methods: Exosomes isolated from four young (Y-Exo) and four old (O-Exo) men were evaluated for CD63 protein expression, morphology, size and zeta potential. HSCs were treated with exosomes, and then, the cell viability and the mRNA expression (hTERT and P16) were evaluated using MTT and qRT-PCR methods, respectively. To measure the hTERT protein level, a western blot technique was performed. Results: The gene expression of hTERT was significantly decreased in HSCs treated with 5 μg/ml (O5-Exo) and 10 μg/ml (O10-Exo) doses of exosomes obtained from elderly individuals compared to the cells treated with young exosomes and the untreated HSCs (p < 0.05). In addition, there was a profound elevation of hTERT protein in the HSCs treated with both doses of young exosomes in comparison with the cells treated with both doses of old exosomes (p < 0.05). Moreover, P16 expression was markedly upregulated in the O5-Exo and O10-Exo groups compared to the untreated group (p < 0.05). Conclusion: Our findings reinforce the concept that depending on the age of individuals, circulating exosomes may acquire properties that affect the pathways involved in the aging process in HSCs.

Influence of different rolling passes and deformation amounts on the microstructure evolution and strengthening mechanism of Cu-Ni-Si alloy

Under the condition of controlling the total rolling deformation to 80.0%, the effects of multi-pass rolling, the ratio of deformation between the first and second stages in two-stage rolling, and aging parameters on the microstructural evolution and property enhancement of Cu-Ni-Si alloy were investigated. The results showed that the second-stage rolling promotes the formation of high-density dislocations, significantly enhancing dislocation strengthening. Simultaneously, the high-density dislocations facilitate the precipitation of the second phase during aging, and concurrently improving the strength and electrical conductivity of the alloy. In the two-stage rolling and aging process, a ratio of first-stage to second-stage rolling deformation less than 1 yields superior comprehensive properties. Specifically, when the first-stage rolling deformation is 35.0% and the second-stage deformation is 68.8%, the alloy achieves an optimal electrical conductivity of 49.53% IACS, hardness of 272.9 HV0.1, tensile strength of 773.0 MPa, and yield strength of 723.3 MPa. This is because a rolling ratio less than 1 results in higher dislocation density; the δ-Ni2Si precipitates have a coherent relationship with the Cu matrix, the interparticle spacing of precipitates is smaller. The average grain size is refined from 1.29 μm to 1.08 μm, significantly enhancing grain boundary strengthening. Dislocation strengthening and second-phase strengthening are the primary strengthening mechanisms of the alloy, and the calculated alloy strength agrees well with the experimental values, with errors less than 3.4%. Additionally, the Avrami equation in phase transformation kinetics was utilized to study the variation law of the volume fraction of the δ-Ni2Si phase with aging time.

Molecular Mechanisms of Polyphenols in Management of Skin Aging.

The natural process of skin aging is influenced by a variety of factors, including oxidative stress, inflammation, collagen degradation, and UV radiation exposure. The potential of polyphenols in controlling skin aging has been the subject of much investigation throughout the years. Due to their complex molecular pathways, polyphenols, a broad class of bioactive substances present in large quantities in plants, have emerged as attractive candidates for skin anti-aging therapies. This review aims to provide a comprehensive overview of the molecular mechanisms through which polyphenols exert their anti-aging effects on the skin. Various chemical mechanisms contribute to reducing skin aging signs and maintaining a vibrant appearance. These mechanisms include UV protection, moisturization, hydration, stimulation of collagen synthesis, antioxidant activity, and anti-inflammatory actions. These mechanisms work together to reduce signs of aging and keep the skin looking youthful. Polyphenols, with their antioxidant properties, are particularly noteworthy. They can neutralize free radicals, lessening oxidative stress that might otherwise cause collagen breakdown and DNA damage. The anti-inflammatory effects of polyphenols are explored, focusing on their ability to suppress pro-inflammatory cytokines and enzymes, thereby alleviating inflammation and its detrimental effects on the skin. Understanding these mechanisms can guide future research and development, leading to the development of innovative polyphenol-based strategies for maintaining healthy skin.

Shape modelling reveals age-related knee bony shape changes in asymptomatic knees.

Osteoarthritis (OA) causes bony shape changes within the knee. Furthermore, the risk of developing OA increases with age. However, age alone does not cause OA. It is therefore important to understand the healthy age-related trajectories of knee shape before attributing these changes to OA. The aim of this study was to determine the association between bony knee shape and age using statistical-shape modelling (SSM). 96 participants received a CT scan of their knee. Three-dimensional models were created using manual segmentation. Separate SSM's for the distal femur and proximal tibia were created. Linear regression models were used to assess the association between age and femoral and tibial shape. Fourteen modes of the femoral and tibial SSM's captured 68% and 73% shape variation, respectively. Only femoral mode 3 and tibial mode 7 were associated with age. Increasing age was related to larger femoral bone volume and deepening of the femoral trochlear groove. Furthermore, increased age was associated with medial tibial plateau expansion. Aspects of bony femoral and tibial shape were significantly associated with aging, including femoral and tibial bone size, femoral trochlear groove, and medial tibial plateau area. Changes in knee morphology occur as a normal process of aging without osteoarthritis development. This may be a response to mechanical loading over time. Further research investigating the effect of these changes on loading in the knee may provide valuable information for knee health in older age.

Mutant Huntingtin Drives Development of an Advantageous Brain Early in Life: Evidence in Support of Antagonistic Pleiotropy.

Huntington's disease (HD) is a neurodegenerative disease caused by a triplet repeat expansion within the gene huntingtin (HTT). Antagonistic pleiotropy is a theory of aging that posits that some genes, facilitating individual fitness early in life through adaptive evolutionary changes, also augment detrimental aging-related processes. Antagonistic pleiotropy theory may explain a positive evolutionary pressure toward functionally advantageous brain development that is vulnerable to rapid degeneration. The current study investigated antagonistic pleiotropy in HD using a years-to-onset paradigm in a unique sample of children and young adults at risk for HD. Cognitive, behavioral, motor, and brain structural measures from premanifest gene-expanded (n = 79) and gene nonexpanded (n = 112) participants (6-21 years) in the Kids-HD study were examined. All measures in the gene-expanded group were modeled using a mixed-effects regression approach to assess years-to-onset-based changes while controlling for normal growth. Simultaneously, structure-function associations were also examined. Decades from motor onset, gene-expanded participants showed significantly better cognitive, behavioral, and motor scores versus gene nonexpanded controls, along with larger cerebral volumes and cortical features. After this initial peak, a prolonged deterioration was observed in both functional and structural measures. Far from onset, brain measures were positively correlated with functional measures, supporting the view that functional advantages were mediated by structural differences. Mutant HTT may drive the development of a larger than normal brain that subserves superior early-life function. These findings support the antagonistic pleiotropy theory of HTT in HD, where this gene drives early advantage followed by accelerated aging processes. ANN NEUROL 2024;96:1006-1019.

Time Since Injury Is Key to Modeling Trends in Aging and Overall Functioning of Persons With Spinal Cord Injury: A Longitudinal Analysis of a Swiss Community Survey.

The aim of the study is to model the health indicator of functioning for persons with spinal cord injury or disorder living in Switzerland in the long-term context, considering chronological age, time since injury, and age at injury. This is a longitudinal analysis of three waves of a community survey. An interval-scaled functioning metric was constructed using modern test theory. Random effects within-between mixed models were used to investigate trends in functioning over time. Persons with complete injuries have lower levels of functioning than persons with incomplete injuries, and persons with complete tetraplegia have the lowest levels of functioning. The person's age when injured, the level and completeness of the injury, the presence of secondary health conditions, and various combinations of these factors significantly influenced how functioning changed over time. Our study shows that chronological age is not necessarily the best time variable for understanding the aging process of people with spinal cord injury in terms of changes in functioning over time and that modeling functioning over time since injury provides important insights into how functioning of people with complete or incomplete injuries declines with increasing time living with the injury.

An Updated Review on the Role of Phytoconstituents in Modulating Signalling Pathways to Combat Skin Ageing: Nature’s Own Weapons and Approaches

Abstract: Various geographical areas exhibit varying degrees of prevalence and severity of dermatological issues. The most commonly observed skin issues among adolescents during their growth period on a global scale encompass dry skin, dyspigmentation, wrinkles, fungal infections, as well as benign and malignant tumors. These conditions arise as a consequence of diminished functional capacity and heightened skin susceptibility. The primary manifestation of the whole process of skin ageing is its visual presentation, which encompasses changes in both the structure and function of the skin. The look and function of human skin exhibit particular variations as individuals age, representing a time-dependent phenomenon. This review article primarily examines the discussion surrounding the diverse phytoconstituents and their impact on signalling pathways in cellular metabolism, as well as their interaction with environmental factors and xenobiotic agents that contribute to skin aging. Ultraviolet (UV) light induces the rapid formation and subsequent accumulation of reactive oxygen species (ROS) within skin cells, hence accelerating oxidative stress and the ageing process of the skin. One effective approach to addressing age-related skin disorders entails the utilization of exogenous supplementation through the consumption of dietary antioxidants, as well as the application of antioxidant-based lotions to the skin prior to sun exposure. Several plant species include phenolic components, including ascorbic acid, ellagitannins, and carotenoids, which have the ability to protect the skin from harmful UV radiation, reduce inflammation and oxidative stress, and influence several survival signalling pathways. This comprehensive study elucidated multiple processes by which phytoconstituents exert their effects for intervention purposes. Additionally, it highlighted the ability of these phytoconstituents to modulate the NF-κB signalling pathway, MAPK signalling, Nrf2 signalling, and other pathways, hence demonstrating their potential anti-aging properties.

ВОЗМОЖНОСТИ ПРИМЕНЕНИЯ НЕИНВАЗИВНЫХ ОФТАЛЬМОЛОГИЧЕСКИХ МЕТОДОВ ДИАГНОСТИКИ ПАТОЛОГИЧЕСКИХ СОСТОЯНИЙ ПРОЦЕССА РАННЕГО СТАРЕНИЯ НА МОДЕЛИ ПРИМАТОВ

Currently, there is an urgent need for early diagnostics, searching for means of predicting the development of cognitive deficit, and assessing the dynamics of changes during treatment. Given the common origin of the brain and eye, which undergo the same onto- and phylogenetic development, some researchers have suggested that the eye can act as a “window” for easily accessible and non-invasive methods for studying brain pathology [3]. The popularity of fundamental research is growing, and interest in creating disease models in primates is becoming more relevant. The creation of such models will significantly expand the possibilities of obtaining the most reliable information, especially in the field of gerontology and brain pathology (cataract, senile ring, age-related changes in the fundus and other signs of early aging). In this part of the work, approaches to studying the fundus, physiological parameters of the structures and functions of the visual organ in rhesus macaques and hamadryas baboons of different ages are developed; potential ophthalmological biomarkers for diagnosing early aging processes are considered. A combined approach using ophthalmological markers, including non-invasive visualization of the retina and detection of pathological proteins in the intraocular fluid, lens or retina, in combination with biomarkers of oxidative stress (malondialdehyde, lipofuscin, protein carbonyls, 8-OH-deoxyguanosine) will allow us to develop a methodology for diagnosing the development and correction of early aging in humans on a primate model.

Depictions of Aging in a Self-Portrait by David Bailly (1584-1657).

I present a case involving a portrait of David Bailly, containing 2 images portraying changes over a period of ∼40 years, and I compare the facial features that illustrate the aging process. He was 67 years old when he painted "Self-Portraits With Vanitas Symbols" (1651). By juxtaposing past representations with present reality, the painting suggests that the young artist is contemplating his future aging. The young man, appearing so vividly within the painting's immediate reality, actually symbolizes a bygone era Compared with the young portrait, the older portrait exhibits a slightly drooped eyebrow tail, indicative of brow ptosis. In the young portrait, the lid margin is aligned with the upper border of the iris, whereas in the older portrait, it is aligned with the upper border of the pupil. The margin reflex distance 1 decreases with age. In addition, the older portrait shows baggy lower eyelids. The nasojugal groove is more pronounced, and the nasolabial fold is deeper. The beard and mustache are more extensive, and silver strands are interspersed among the black hairs. Presenting 2 images from a 40-year interval, the older portrait exhibits more pronounced signs of facial aging than the younger portrait. Portraits from that era, whether painted by western or eastern artists, demonstrate that the painters had an understanding of facial skin lines and the aging process. The process of creating portraits provides insights into historical contexts and remains significant in contemporary settings, particularly concerning perceptions and identity.

Factors affecting ageism and depression levels in individuals aged 65 and over living in society

Abstract Background The aging process is perceived negatively by some people. This perception prevents/reduces the happiness that can be gained from the aging process. As a result, depressive symptoms may occur. Therefore, this study aimed to examine the factors affecting ageism and depression levels in individuals aged 65 and over living in society. Methods The sample of the research, which was conducted with a cross-sectional, descriptive design, consisted of 287 individuals aged 65 and over living in a neighborhood on the Anatolian side of Istanbul. Data were collected with socio-demographic form, Ageism Questionnaire and Yesavage Geriatric Depression Scale-15. T test, variance analysis and chi-square test were used to analyze the data. Ethical permissions were obtained. Results Moderate depression was found in 27.5% of individuals with a mean age of 70.80±5.42. Individuals scored below the average (18.48±8.14) on the ageism survey. Ageism scores were higher for men, those aged 85 and over, those with poor economic conditions, those with chronic diseases, those hospitalized in the last year, those with severe and moderate depression, and those who needed help with daily tasks (p < 0.05). A statistically significant difference was found between age groups, chronic disease, continuous medication use, hospitalization in the last year, working status, need for help with daily tasks, economic status and depression levels (p < 0.05). Ageism scores of those with severe and moderate depression were found to be high (F = 3.43;p=0.017). Conclusions Variables that are effective in ageism and depression are being aged 85 and over, having a poor economic situation, having a chronic disease, needing help with daily tasks, and receiving inpatient treatment in the last year. The results of this study show that more efforts and resources are needed within the scope of political and social services to reduce ageism in society and the level of depression in older individuals. Key messages • As the level of depression in elderly individuals increases, ageism increases. • Advanced age, economic status, chronic disease, hospitalization and dependence on daily tasks affect depression and ageism.

Intersecting Pathways: Understanding Dementia, Hearing Loss, and Health Disparities

Abstract Background Dementia is a major public health concern, affecting care dependency and disability in older ages. In Europe, around 14.1 million people live with dementia, a number that is expected to increase to 15.9 million by 2040. Hearing loss (HL), followed by education, has been proposed as the most important modifiable risk factor for dementia. This study aims to investigate how sex, educational attainment and HL intersect to influence dementia-free life expectancy (DemFLE) in older adults. Methods Using population register data covering all Finnish residents aged 50 years or older from 2010 to 2019, we apply discrete-time multistate Markov models. Transition probabilities between states, such as HL and dementia, are estimated with multinomial logit models stratified by sex and education. Multistate life tables are used to compute sex- and education-specific DemFLE. Results We identified 316,361 individuals with a diagnosis of dementia and 317,115 individuals with a diagnosis of HL, representing 1,449,873 and 2,738,970 person-years respectively. 14% of individuals with HL were eventually diagnosed with dementia, compared to only 8% of individuals without HL. We hypothesise that DemFLE will vary significantly by sex and education, with additional modulation by the presence of HL. Specifically, we expect that the presence of HL will further reduce DemFLE, and that the magnitude of this reduction will be relatively greater among those with lower educational attainment. Conclusions This study contributes to the emerging literature on the impact of hearing loss on dementia. While both dementia and hearing loss are irreversible health conditions, hearing loss can be managed by hearing aids. If we find evidence that hearing loss is associated with different levels of risk of dementia by educational attainment, it would suggest avenues for effective interventions and public health policies to ensure more equal ageing processes in the population. Key messages • Hearing loss may intersect with education and sex in the risk of dementia, providing evidence for the importance of addressing hearing health inequalities in dementia prevention efforts. • Understanding the interactions between hearing loss, education and sex can inform policies to promote healthy ageing and reduce the burden of dementia on individuals and health systems.

10.I. Scientific session: A National E-Infrastructure for Aging Research to Improve Public Health

Abstract Global life expectancy gains over the last half-century are remarkable. Yet the resulting demographic shift towards an older population poses substantial public health challenges, particularly in healthcare. To address these challenges, a profound and scientifically-based understanding of older adults’ health and care requirements is needed. Recognizing the need for a better understanding of the aging process and public health challenges, the National E-infrastructure for Aging Research (NEAR) was founded in 2018. NEAR seeks to promote and support aging research by facilitating longitudinal population-based data usage from 15 well-known Swedish cohort studies on aging and health. It is based on a collaboration between eight Swedish universities and includes 15 databases with individual assessments. NEAR comprises a broad range of biomedical, clinical, social, and psychological health data from more than 90,000 adults who are 50+ years old. These individuals have been followed between 12 and 52 years. The objective of this workshop is to give an overview of the NEAR infrastructure, present results from three ongoing NEAR projects, and their implications for public health: 1) Temporal trends in dementia incidence in Sweden; 2) Personality and biomarkers of neurodegeneration and Alzheimer’s disease in Swedish older adults, and 3) Characterizing risk profiles and transitions across living and care settings in Sweden. Infrastructures such as NEAR offer high-quality epidemiological data for achieving broad, interdisciplinary research goals that single databases often cannot provide. Moreover, the value added to these kinds of data includes enhanced sample sizes, variations, representativeness, and generalizability. In turn, this can address an aging society’s public and clinical health demands. Eventually, this could lead to sustainable intervention strategies for improving older persons’ health in the coming decades. Key messages • Discover new avenues in public health aging research: Gain insights into cutting-edge aging research opportunities in Sweden, exploring key NEAR projects. • Recognize the value of national infrastructures using high-quality epidemiological data in fostering collaborative and nationally representative public health research.

Clinical prediction model of main adverse cardiovascular and cerebrovascular events for elderly patients with acute coronary syndrome

Abstract Background Age is an independent factor for adverse outcomes in acute coronary syndrome (ACS). With the accelerated aging process, there is an increasing incidence of ACS in the elderly; however, research on this population is underrepresented. Due to the unique presence of multiple diseases and organ changes in the elderly, specialized risk assessment tools are required. Purpose To explore the risk factors for poor prognosis of elderly patients with ACS and establishing a risk stratification tool. Methods The derivation cohort consisted of 1674 elderly ACS patients consecutively enrolled in a single center from January 2013 to December 2013. The validation cohort included 2333 elderly ACS patients consecutively enrolled in nine medical centers from January 2015 to May 2019. All patients were followed up for 2 years, and the study endpoint was defined as major adverse cardiovascular and cerebrovascular events (MACCE). Model selection was performed using Cox regression and the Akaike Information Criterion (AIC) to construct the final risk assessment model. The performance of the predictive model was evaluated in the derivation and validation cohorts using the c-index, receiver operating characteristic (ROC) curves, and calibration curves. Results A total of 1,674 elderly ACS patients (70.97±4.68 years, 23.24% female) were included in the derivation cohort, and 210 cases (12.54%) of MACCE were recorded during the 2-year follow-up period. In the validation cohort, there were 2,333 elderly ACS patients (72.13±5.82 years, 35.83% female), with 364 cases (15.60%) of MACCE documented during the 2-year follow-up. Initially, 25 variables were preliminarily selected based on the results of univariate Cox regression and the clinical importance of these variables. Subsequently, these variables were included in a multivariable Cox model, and model selection was performed based on the AIC, resulting in the final selection of 10 variables (including age, cardiac insufficiency, current smoker, DAPT use, history of PCI or CABG, IABP use, number of lesions, residual SYNTAX score, preoperative HGB, and preoperative PLT). Then we developed and validated a nomogram to predict the 2-year MACCE in elderly ACS patients based on the assessment of 10 clinically easily obtainable variables during hospitalization. In both the derivation and validation cohorts, the nomogram demonstrated good discriminative ability (AUC of 0.723 and 0.699, c-index of 0.727 and 0.661, respectively) and calibration (calibration curves closely approximating the 45-degree diagonal line). The Kaplan-Meier analysis reveals significant differences in MACCE incidence rates between groups stratified by the median predicted probability (p<0.001). Conclusion Our study developed and validated a practical nomogram for predicting the 2-year risk of MACCE in elderly patients with ACS.

Otolith assessment in elderly using combined vestibular-evoked myogenic potentials and subjective visual vertical test

BackgroundDizziness in elderly patients is classified as a multifactorial geriatric syndrome leading to pathological conditions or changes endemic to the aging process, it is characterized by increased susceptibility to accidents and instability. This is considered a major public health problem. In our study, we assessed age related changes in vestibular function via measurement of combined Vestibular-Evoked Myogenic potentials (VEMPs) as well as Static Subjective Visual Vertical Test (SVVT) for otolith function.MethodsSeventy-five subjects (150 ears) were enrolled in this research. They were categorized into a control group composed of twenty-five adults from 18–40 years and a study group which divided into symptomatic and asymptomatic groups, each group consisted of twenty-five subjects, fifteen subjects aged 55- < 65 years & ten subjects aged 65 to 85 years. All subjects were submitted to combined VEMPs and static SVVT.ResultsDetectability of combined VEMPs decreased with age with significant difference among the older age group (> 65- < 85 years) and the subjects aged (55- < 65 years). Percentage of abnormal cVEMPs & oVEMPs latencies and amplitudes was more detected in the older age group than subjects aged (55- < 65 years). The symptomatic group aged > 65 years showed higher percentage of affection of both cVEMPs & oVEMPs, while the younger group aged 55- < 65 showed higher absence of bilateral oVEMPs. There were no statistically significant differences in the asymmetrical ratio of cVEMPs & oVEMPs between elderly and adults. Static SVV perception does not change with age.ConclusionsThe elderly showed variable degrees of otolith dysfunction with a high percentage of affection in cVEMPs and oVEMPs. Also, they showed symptoms of imbalance and light-headedness. Static SVVT perception doesn’t change with age.

ND15, subunit of the electron transport chain protein-complex I, age-dependently worsens cardiac performance in Drosophila melanogaster

Abstract Introduction In the natural aging process of the heart, aside others, mitochondrial dysfunction is an essential driver for the development of reduced cardiac performance. Previous analysis of protein expression levels in cardiomyocytes of aged mice and have found aberrancies in subunits of the mitochondrial Complexes I – IV of the electron transport chain. Especially, the complex I subunit ND15 was highly downregulated. To elucidate the functional effects of this gene on the cardiac performance, we generated cardiomyocyte specific ND15 knock-downs in Drosophila melanogaster. Methods We generated Drosophila with heart-specific (+/+; Hand4.2-GAL4/UAS-ND15; tdtK/+) (ND15-KD)knockdown of ND15 using the UAS/GAL4 system. The flies' cardiomyocytes endogenously express tdTomato, facilitating fluorescence-based live imaging of the heart by high-resolution video fluorescence microscopy. The recorded data was processed using a R-Script, resulting in Results We compared wild type (WT) flies to ND15-KD flies at age 3 weeks(young) and 7 weeks(old). Knockdown of ND15 resulted in impaired cardiac function. Most parameters deteriorated in aged flies in comparison to young flies. The heart frequency was significantly reduced (WT:6 beats/s – KD:4 beats/s – p&amp;lt;0.0001) in the 7 weeks old group, end-diastolic diameter of the heart tube increased significantly in 3 weeks old ND15KD flies compared to controls(WT:65µm – KD:74µm p&amp;lt;0,0001), resulting in a dilated heart tube Interestingly enough, this dilation in ND15-KD disappeared in 7 weeks old flies. The fractional shortening was significantly decreased in young(WT:33,5% - KD:30% p&amp;lt;0,01) and deteriorated in old flies (WT:35% - KD:22% p&amp;lt;0,0001), indicating a reduced contraction capability. For both 3 weeks and 7 weeks old groups, the heart-rate adjusted interbeat interval variance (WT:22% -KD: 40% p&amp;lt;0,0001) as well as the time of no cardiac action (WT: 22% - KD: 35% p&amp;lt;0,0001) increased and showed significant irregularities. The duration of systole and diastole combined also exhibited arrhythmic patterns (WT:15% - KD:20.5% p&amp;lt;0,0001). A general arrhythmia index (AI) increased significantly (WT: 0.25 – KD:0.5 p&amp;lt;0,0001) These arrhythmia parameters emphasize the arrhythmogenic potential of the ND15-knockdown. The relative relaxation capabilities drastically worsened with age, displaying a diastolic dysfunction in the 7 weeks old group. At half-time relaxation, the WT was at 60% relaxation, ND15KD at 46%). Conclusion This study demonstrates that heart-specific deletion of ND15 results in a marked impairment of systolic and diastolic cardiac function in Drosophila accompanied by pro-arrhythmogenic actions. We could identify ND15 as a new mitochondrial molecular target for the maintenance of regular cardiac function and rhythmicity, especially with increasing age. Further mechanistic studies and translation to mammalian organisms are ongoing.

1.L. Scientific session: Public Health data in support of healthy ageing policies

Abstract As Europe grapples with an ageing population, the imperative of health policies that promote healthy ageing is increasingly critical. This workshop aims to explore the significant role of public health data in shaping and supporting such policies. By integrating insights from administrative records to sophisticated research infrastructures, we will highlight the multifaceted uses of data in public health, particularly in the realms of non-communicable diseases and health-related behaviours in older populations. The session will open by examining how administrative records have been utilised within Lombardy’s regional health system to understand the epidemiology and access to care for people living with dementia. The discussion will then cover the advantages and challenges associated with integrating health data from diverse sources, emphasising the importance of quality, privacy, and the potential for real-time surveillance and response systems in healthcare. Following this, the focus will shift to the determinants of good health among older adults, presenting findings from a comprehensive cross-sectional study that identifies key factors contributing to what can be termed as “superhero” health status: seniors who maintain excellent physical and mental health. This analysis will delve into socioeconomic, behavioural, and environmental factors that influence health outcomes in the elderly, providing evidence-based recommendations for public health interventions and highlighting the potentialities of ad hoc surveys in examining health outcomes during emergencies. Thirdly, the workshop will also address the impact of significant life transitions on health behaviours, specifically the transition to retirement and its effects on alcohol consumption. Leveraging longitudinal data from a pan-European multidisciplinary research infrastructure, it will be demonstrated how retirement affects daily habits and lifestyles, including changes in alcohol consumption patterns. This segment will explore the implications of these findings for public health planning and policy formulation. In conclusion, there will be a discussion about healthcare system policies that support healthy ageing and the global impact of ageing on economics and health economics. These discussions will draw from the presented data sources and studies to suggest ways in which public health policies can be designed to reduce disparities and improve overall health outcomes among the elderly. This workshop is designed to provide a comprehensive overview of how data-driven insights can inform effective public health strategies and interventions to support an ageing society. By exploring various aspects of health data application, from individual behaviour to systemic policy adjustments, the session aims to highlight the critical role of data in promoting sustainable health and well-being in older adults, especially in the context of increasing life expectancy and prolonged retirement periods. Key messages • Harnessing data from diverse sources is crucial for understanding and enhancing healthy ageing processes. • Utilising longitudinal studies and robust data to design informed public health policies is essential for effective ageing interventions.

Assessing the utility of computer vision for age determination of Gulf Menhaden

AbstractObjectiveIn this work, we assess the potential of computer vision techniques for age estimation of Gulf Menhaden Brevoortia patronus scales. Scales are the primary structure used for the age determination of Gulf Menhaden, and the ageing process can be labor intensive. Gulf Menhaden is the second‐largest fishery by weight in the United States, with average annual landings from 2018 to 2022 of 449,540 metric tons, and is assessed with age‐structured models that require information about the age structure of the catch and the stock.MethodsWe used convolutional neural networks and deep neural networks to classify the age from images of Gulf Menhaden scales from three different sets of images of scales. The first set of data consists of images of scales from fish at ages 0 and 1 year. The second set of data consists of images of scales from fish at ages 0–4 years. The last set of data consists of images of scales from fish of ages 0, 1, and 2 years and includes only images of scales for which there is agreement by readers of age estimates derived from analyzing sagittal otoliths and scales from the same individual.ResultThe classification of ages was best when using a convolutional neural network model on the first data set. The poorest classification was for the model using deep neural networks with the second data set.ConclusionAlthough we show that computer vision has promise for age determination from fish scale samples, our results indicate that considerable work must be done for wide adoption of the approach. With the continuous enhancements of computer vision models, improvements in the quality of scale images, and the accumulation of larger sets of scale images that can be used to train machine learning models, we believe that using computer vision can serve to reduce processing time and increase the accuracy of age estimates.