Pollination Process Research Articles

Ecological Role of Spiders in Terrestrial Ecosystem and Their Conservation Priorities

Spiders are members of the phylum Arthropoda, the largest phylum of animal groups in the world under the class Arachnida. Spiders are ubiquitous in distribution, invade almost every type of habitat, and are the most abundant invertebrate predators in terrestrial ecosystems. Nearly 47,617species of spiders under 4,092 genera in 114 families were recorded in the world. Spiders are a key component of terrestrial ecosystems, occupying a unique position in food webs. As predators, they are important in the regulation of invertebrate populations, which include pest species, and as prey, they provide food for other invertebrates and birds. They are important components of the food chain. This is not withstanding the contribution they make to ecosystems in their own right.Spider silk has unique properties that have potential applications in medicine and technology. Many species of spiders have been used as medicines. As bee populations plummet, spiders might be the next solution to the pollination process. Spiders are among the most fascinating creatures on earth, and their importance in scientific research cannot be overstated. Spiders play a critical role in maintaining ecological balance, and studying their behavior and biology can greatly advance our understanding of the natural world. The witnessing role of spiders in terrestrial ecosystems has made them ecologically important. In the past few years, spiders have faced several threats that have affected their populations and diversity. Prior conservation programs are required to protect this species from being more vulnerable and endangered.

Robust pollination for tomato farming using deep learning and visual servoing

Abstract In this work, we propose a novel approach for tomato pollination that utilizes visual servo control. The objective is to meet the growing demand for automated robotic pollinators to overcome the decline in bee populations. Our approach focuses on addressing this challenge by leveraging visual servo control to guide the pollination process. The proposed method leverages deep learning to estimate the orientations and depth of detected flower, incorporating CAD-based synthetic images to ensure dataset diversity. By utilizing a 3D camera, the system accurately estimates flower depth information for visual servoing. The robustness of the approach is validated through experiments conducted in a laboratory environment with a 3D printed tomato flower plant. The results demonstrate a high detection rate, with a mean average precision of 91.2 %. Furthermore, the average depth error for accurately localizing the pollination target is impressively minimal, measuring only 1.1 cm. This research presents a promising solution for tomato pollination, showcasing the effectiveness of visual-guided servo control and its potential to address the challenges posed by diminishing bee populations in greenhouses.

Into the Buzzing World: Integrating Artwork with Fieldwork to Learn About Bees

This article describes a pedagogical approach that integrated artistic expression with experiential fieldwork to enhance pupils’ learning of bees. Initially, pupils were tasked with creating an artwork of a bee or bees based on their preconceived notions, allowing teachers to identify their prior ideas of bees. Afterward, an immersive field activity was conducted to provide pupils with an opportunity to close-up observe live honey and native bees in canola fields and their pollination processes. Through teacher-guided explorations, pupils systematically inspected the morphology of various bee species, relating their external structures to associated functions. The fieldwork allowed for detailed observations of bees’ features, such as the proboscis, antennae, and legs, and their roles in nectar collection and pollen distribution. In addition, exposure to honey and native bees informed pupils of the differences in their stinging, nesting and sociality. Pupils’ summative reflections post-fieldwork suggest that the immersive fieldwork enabled multi-sensory modes of learning barely available in a traditional classroom setting. By juxtaposing the new insights from field observations with the prior ideas of bees embodied in their initial artworks, pupils achieved gains in both their conceptual understandings and perceptual knowledge about bees. Pupils acknowledged deeper understandings of bee anatomy, pollination processes and the diversity within bee species. This approach to learning about bees epitomizes the value of empirical investigation for advancing pupils’ scientific practices and understanding life science core ideas of how animals' external structures fulfill various functions and of how plants and pollinators are interdependent, as highlighted in <i>the Next Generation Science Standards</i>. The integration of artwork creation and experiential fieldwork exemplifies an effective pedagogical approach for enhancing pupils' engagement and learning of biological ideas.

Glyphosate contact alters the expression of genes in the head of africanized Apis mellifera bees

The increase in the world population results in a greater demand for food, intensifying the dependence on pesticides use. Glyphosate, a pesticide widely used in crops, can contaminate resources collected by bees. In this context, this study aimed to analyze the transcriptome of the head of Africanized Apis mellifera bees during the foraging phase to determine whether contact with the herbicide glyphosate, in lethal and sublethal doses, causes changes in the gene expression profile. For this, 180 marked bees were collected from their hives, aged 21 days, and distributed into three treatment groups: 1) bees in contact with the lethal dose of glyphosate (255.73 µg/bee); 2) bees in contact with the sublethal dose of glyphosate (2.5573 µg/bee); 3) control group with distilled water. Transcriptome analysis was performed on 12 bees from each treatment after one and four hours of exposure. The results show that one hour of contact with glyphosate resulted in 57 genes presenting differential expression in relation to the control (11 lethal and 53 sublethal), interfering in the signaling, communication and metabolism processes, while four hours of exposure, 38 genes (32 lethal and 11 sublethal) related, mainly, to the stimulation of the immune response. In summary, glyphosate affects the gene expression of honey bees during the foraging phase, at both doses, resulting in negative effects on behavior, metabolism and immune system, which compromises the health, activity and development of the colonies, also compromising the process of pollination.

Effect of gradual pollen presentation on pollination efficiency and reproduction of Vaccinium corymbosum Berkeley in two habitats

IntroductionGradual pollen presentation is a reproductive strategy for plants to improve the efficiency and accuracy of pollen export and acceptance as well as to promote outcrossing. Vaccinium corymbosum Berkeley has a typical mechanism of gradual pollen presentation.MethodsIn this study, we used indoor and outdoor observations to investigate the floral characteristics, pollination characteristics and breeding system of this species in open ground and greenhouse.ResultsThe results showed that the single white, inverted bell-shaped flower of V. corymbosum was able to effectively avoid the negative effects of rainfall, nectar dilution and pollen removal by wind, and improved pollen viability and the duration of stigma receptivity. The setting rate of ‘Northland’ is higher than open ground by artificial pollination in greenhouse, therefore, greenhouses are more suitable for ‘Northland’ reproduction. The gradual pollen presentation prolongs dispersal time, promotes pollinator participation in the pollination process, increases staminate fitness, and provides for nectar feeding by pollinators as well as pollen export. Bombus spp. and A. m. ligustica were effective pollinators in the open ground and greenhouse, and the pollination efficiency of Bombus spp. was higher than that of A. m. ligustica, and greenhouse pollination is more efficient than open ground. The coincidence of the peak period of pollinator visits with the period of highest pollen viability and optimal pollination of stigmas, as well as the high nectar production, could maximize the frequency of pollinator visits and increase the chances of successful pollination as well as the pollination efficiency.DiscussionTherefore, it is important to study the floral characteristics of V. corymbosum Berkeley and the mechanism of gradual pollen presentation in order to improve the pollination efficiency and promote the success of outcrossing, which can provide a theoretical basis for the breeding of V. corymbosum.

Optimization of the method of Agrobacterium-mediated in planta transformation of winter wheat genotypes

Aim. Optimization of the Agrobacterium-mediated in planta transformation of winter wheat genotypes. Methods. Genetic transformation, molecular genetic analysis; of mathematical statistics. Results. The influence of the composition of the inoculation medium and the method of application of the agrobacterium cell suspension on the frequency of seed setting and the formation of transgenic plants of new promising genotypes of winter wheat was studied. The dependence of obtaining the amount of grain and transformants on the genotypic characteristics of plants when using different inoculation conditions and transformation procedures was established. The higher efficiency of the MC-32 inoculation medium, which additionally contained MES, magnesium sulfate, and ammonium chloride, was shown compared to the MC-31 medium supplemented with sodium thiosulfate. A significant difference in the rate of seed setting was revealed when using different methods of applying A. tumefacins cell suspension. Method I proved to be the most effective, when a bacterial culture was applied to the pistil receptacle, and after the liquid in which the agrobacteria were resuspended completely dried, pollination was carried out with pollen obtained from an intact ear of the same plant. Conclusions. Selected inoculation conditions for efficient gene transfer in the pollination process for Agrobacterium-mediated in planta transformation of new winter wheat genotypes.

Особенности развития генеративных структур некоторых цветковых растений

The paper presents the results of a comparative study of the reproductive biology of a number of Crimean flora species and valuable plants introduced to the south of Russia belonging to various families (Magnoliaceae, Annonaceae, Ericaceae, Campanulaceae, Anacardiaceae, Lamiaceae, Ranunculaceae, Davidiaceae, Nyssaceae, Onagraceae, Papaveraceae), which made it possible to establish some features and regularities in the formation of the generative structures of the reproductive system, consisting in the conjugation of the development of male and female elements, the maturation of sexual gametes and the process of pollination in entomophilous plants. A conclusion was drawn concerning the high reproductive potential of the studied species, which is expressed in the formation of a sufficient number of full-fledged seeds to reproduce. The possibilities of using the knowledge of the reproductive processes of flowering plants in solving certain issues of taxonomy, determining the strategy of reproduction and conservation of phytodiversity, and showing the importance of antecological aspects in plant reproduction, breeding and nature protection were demonstrated. The material is of scientific interest to botanists, ecologists, breeders and other specialists.

Deep learning approach for detecting tomato flowers and buds in greenhouses on 3P2R gantry robot

In recent years, significant advancements have been made in the field of smart greenhouses, particularly in the application of computer vision and robotics for pollinating flowers. Robotic pollination offers several benefits, including reduced labor requirements and preservation of costly pollen through artificial tomato pollination. However, previous studies have primarily focused on the labeling and detection of tomato flowers alone. Therefore, the objective of this study was to develop a comprehensive methodology for simultaneously labeling, training, and detecting tomato flowers specifically tailored for robotic pollination. To achieve this, transfer learning techniques were employed using well-known models, namely YOLOv5 and the recently introduced YOLOv8, for tomato flower detection. The performance of both models was evaluated using the same image dataset, and a comparison was made based on their Average Precision (AP) scores to determine the superior model. The results indicated that YOLOv8 achieved a higher mean AP (mAP) of 92.6% in tomato flower and bud detection, outperforming YOLOv5 with 91.2%. Notably, YOLOv8 also demonstrated an inference speed of 0.7 ms when considering an image size of 1920×1080\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$1920 \ imes 1080$$\\end{document} pixels resized to 640×640\\documentclass[12pt]{minimal} \\usepackage{amsmath} \\usepackage{wasysym} \\usepackage{amsfonts} \\usepackage{amssymb} \\usepackage{amsbsy} \\usepackage{mathrsfs} \\usepackage{upgreek} \\setlength{\\oddsidemargin}{-69pt} \\begin{document}$$640 \ imes 640$$\\end{document} pixels during detection. The image dataset was acquired during both morning and evening periods to minimize the impact of lighting conditions on the detection model. These findings highlight the potential of YOLOv8 for real-time detection of tomato flowers and buds, enabling further estimation of flower blooming peaks and facilitating robotic pollination. In the context of robotic pollination, the study also focuses on the deployment of the proposed detection model on the 3P2R gantry robot. The study introduces a kinematic model and a modified circuit for the gantry robot. The position-based visual servoing method is employed to approach the detected flower during the pollination process. The effectiveness of the proposed visual servoing approach is validated in both un-clustered and clustered plant environments in the laboratory setting. Additionally, this study provides valuable theoretical and practical insights for specialists in the field of greenhouse systems, particularly in the design of flower detection algorithms using computer vision and its deployment in robotic systems used in greenhouses.

Семенная продуктивность и качество семян Pinus sylvestris L. в лесных культурах на крымских яйлах

The seed production and quality of Pinus sylvestris seeds in forest plantations on the Crimean yaylas has been studied. The dynamics of biometric indicators of cones and seeds by year and in connection with the peculiarities of growing conditions has been characterized. Significant differences in the quality of seeds of P. sylvestris trees have been revealed in terms of the level of germination energy. It has been shown that, along with the temperature regime, a significant impact on the effectiveness of P. sylvestris reproduction processes is exerted by air humidity and the nature of precipitation, as well as the dynamics of air mass movement, since the success of pollination is determined by the quantity and quality of pollen that has reached the female reproductive structures. Based on the analysis of weather phenomena and the dynamics of seed quality, it has been found that the level of seed germination is best reflected by an integrated index that takes into account the combined effect of several factors – the dryness index. In addition, the results of the analysis allowed us to conclude that the efficiency of the pollination process of female cones of P. sylvestris under yayla conditions is most likely influenced by the number of hours of sunshine. The germination energy and seed germination differ not only within the studied plantations, but also depending on the year of study. During the observation period, the forest plantations of the Ai-Petrinskaya Yayla have produced the highest quality seeds. In 2014, in the plantations with a density of 0.5, the seed germination rate was 74.5±2.2 %. The lowest level of germination energy and seed germination has been noted in artificial plantations of P. sylvestris on the Tirke Yayla; in 2012, these indicators here amounted to 15.2±0.7 and 46.2±2.1 %, respectively. In general, the development of the generative sphere of P. sylvestris in artificial plantations on the Crimean yaylas is quite effective. Based on this, a conclusion has been made that the growing conditions on the yaylas are favourable for the implementation of the initial stages of the ontogenesis of the species.

Disruption of pollination by herbivores is rescued by nectar yeasts

Abstract Attracting pollinators to achieve successful reproduction is a key challenge for wild plants that may be disturbed by complex multispecies interactions in nature. Pairwise plant–pollinator interactions have traditionally been studied for decades, while ignoring other ecological players may obscure a comprehensive understanding on how plants recruit partners or combat enemies in the pollination process. Hence, integrated studies considering the inherent complexity of ecological interactions are needed, which may open up new perspectives for deciphering intricate systems and predicting ecological consequences. We examined the presence of nectar yeasts using a combination of high‐throughput sequencing, cultivation and microscopy and quantified floral herbivory by evaluating the incidence of flowers with visible holes in 13 natural populations of Iris bulleyana in the Hengduan Mountains of southwest China during 2017–2022. We combined yeast inoculation and herbivore manipulation treatments to illustrate the isolated and combined impacts of two contrasting nectarivorous organisms, the ascomycetous yeast Metschnikowia reukaufii and adult sawflies, on pollinator visitation and plant reproductive success in two populations. In the lab, we first employed gas chromatography–mass spectrometry to profile the volatile metabolites of yeast‐inoculated nectar relative to control, followed by a behavioural bioassay to test the preference of honeybees for these microbial volatiles. Yeasts commonly inhabited floral nectar and insect herbivores frequently bit holes in the perianth tube to consume nectar and nectaries. Nectar yeasts indirectly facilitated plant reproduction through increased pollinator visits, probably because of microbial metabolism as honeybees preferred nectar volatiles produced by yeasts in behavioural bioassays. Insect herbivores increased total floral visits but reduced legitimate visits by inducing legitimate‐to‐robbing behavioural changes of honeybees, thus leading to lower seed production. The detrimental effect of herbivory was mitigated by the presence of yeasts, which diminished the relative proportion of robbing visits and thereby ‘rescued’ flowers from reproductive failure. Synthesis: Overall, we found contrasting effects of non‐pollinator species, including both micro‐ and macro‐organisms, on plant–pollinator interactions in a biodiversity hotspot, where pollination deficit may be a ubiquitous phenomenon. Our findings suggest that both microbial and herbivory effects are likely to be important in explaining the exact causes of pollen limitation in species‐rich areas, highlighting the biological context dependence of species interactions in natural ecosystems.

Keanekaragaman dan Status Konservasi Burung di Kawasan Gili Meno, Lombok Utara

The presence of birds in Gili Meno has a positive impact on environmental aspects as birds serve as bioindicators of environmental quality, pest controllers, and aid in pollination processes. Based on research conducted in 2010, 2011, 2012, and 2015, there has been an increase in bird populations in the Gili Meno area, particularly birds from the Ardeidae family. With rapid development currently underway, it is suspected to affect the presence of birds in Gili Meno, necessitating monitoring of bird diversity and conservation status in the area. This study aims to assess the diversity and conservation status of birds in the Gili Meno area. The research was conducted on Gili Meno from May to June 2022. Data collection utilized snowball sampling for interviews and transect methods combined with point counts. Observational data were analyzed using the Shannon-Wiener diversity index. The results indicate a total of 29 bird species found in the Gili Meno area, belonging to 22 families, with a diversity index of 2.8. The conservation status of most birds (97%) in the Gili Meno area is categorized as "least concern" according to IUCN criteria and classified as "unprotected" species under P.106/MENLHK/Setjen/Kum.1/12/2018. Acridotheres javanicus is listed as vulnerable by IUCN, while Thallaseus bergii is protected under P.106/MENLHK/Setjen/Kum.1/12/2018.

Pollination Ecology, Breeding System, and Conservation of Butia lallemantii Deble & Marchiori (Arecaceae): A Useful Dwarf Palm Tree from the Pampa.

The Dwarf Palm, Butia lallemantii Deble & Marchiori, is an endangered species endemic to the Pampa biome and typically grows in sandy and rocky soils. Given its economic, ecological, and cultural relevance, it is crucial to understand the ecology and biology of this species to encourage its preservation and highlight its significance for the Pampa. This study aims to investigate whether this palm relies on animal vectors for pollination, analyze its breeding system, and propose strategies for its conservation and sustainable use. We conducted field observations on pollination ecology, identified floral visitors, and designed six breeding system experiments to test cross-compatibility, self-compatibility, and apomixis. Additionally, we conducted a literature review to propose conservation strategies. Butia lallemantii is pollinator-dependent and self-compatible. The flowers are mostly melittophilous and offer pollen and nectar for floral visitors. The main pollinators are native Meliponinae and Halictinae bees and the introduced Apis mellifera. This study represents the first comprehensive and complete examination of the breeding system and pollination process on Butia palms. This palm can provide materials for industries, but urgent actions are needed to preserve the remaining populations through effective policies and strategies. Furthermore, this palm should be integrated into diversified agroecosystems to evaluate its adaptability to cultivation.

Differential impacts of land‐use change on multiple components of common milkweed (Asclepias syriaca) pollination success

AbstractLand‐use change is one the greatest threats to biodiversity and is projected to increase in magnitude in the coming years, stressing the importance of better understanding how land‐use change may affect vital ecosystem services, such as pollination. Past studies on the impact of land‐use change have largely focused on only one aspect of the pollination process (e.g., pollinator composition, pollinator visitation, and pollen transfer), potentially misrepresenting the full complexity of land‐use effects on pollination services. Evaluating the impacts across multiple components of the pollination process can also help pinpoint the underlying mechanisms driving land‐use change effects. This study evaluates how land‐use change affects multiple aspects of the pollination process in common milkweed populations, including pollinator community composition, pollinator visitation rate, pollen removal, and pollen deposition. Overall, land‐use change altered floral visitor composition, with small bees having a larger presence in developed areas. Insect visitation rate and pollen removal were also higher in more developed areas, perhaps suggesting a positive impact of land‐use change. However, pollen deposition did not differ between developed and undeveloped sites. Our findings highlight the complexity evaluating land‐use change effects on pollination, as these likely depend on the specific aspect of pollination evaluated and on the of the intensity of disturbance. Our study stresses the importance of evaluating multiple components of the pollination process in order to fully understand overall effects and mechanisms underlying land‐use change effects on this vital ecosystem service.

MORN motif-containing protein OsMORN1 and OsMORN2 are crucial for rice pollen viability and cold tolerance.

The pollen viability directly affects the pollination process and the ultimate grain yield of rice. Here, we identified that the MORN motif-containing proteins, OsMORN1 and OsMORN2, had a crucial role in maintaining pollen fertility. Compared with the wild type (WT), the pollen viability of the osmorn1 and osmorn2 mutants was reduced, and pollen germination was abnormal, resulting in significantly lower spikelet fertility, seed-setting rate, and grain yield per plant. Further investigation revealed that OsMORN1 was localized to the Golgi apparatus and lipid droplets. Lipids associated with pollen viability underwent alterations in osmorn mutants, such as the diacylglyceride (18:3_18:3) was 5.1-fold higher and digalactosyldiacylglycerol (18:2_18:2) was 5.2-fold lower in osmorn1, while the triacylglycerol (TG) (16:0_18:2_18:3) was 8.3-fold higher and TG (16:0_18:1_18:3) was 8.5-fold lower in osmorn2 than those in WT. Furthermore, the OsMORN1/2 was found to be associated with rice cold tolerance, as osmorn1 and osmorn2 mutants were more sensitive to chilling stress than WT. The mutants displayed increased hydrogen peroxide accumulation, reduced antioxidant enzyme activities, elevated malondialdehyde content, and a significantly decreased seedling survival rate. Lipidomics analysis revealed distinct alterations in lipids under low temperature, highlighting significant changes in TG (18:2_18:3_18:3) and TG (18:4_18:2_18:2) in osmorn1, TG (16:0_18:2_18:2) and PI (17:2_18:3) in osmorn2 compared to the WT. Therefore, it suggested that OsMORN1 and OsMORN2 regulate both pollen viability and cold tolerance through maintaining lipid homeostasis.

Characterization of Optical, Thermal, and Viscoelastic Properties of Pollenkitt in Angiosperm Pollen Using In-Line Digital Holographic Microscopy.

Pollen grains are remarkable material composites, with various organelles in their fragile interior protected by a strong shell made of sporopollenin. The outermost layer of angiosperm pollen grains contains a lipid-rich substance called pollenkitt, which is a natural bioadhesive that helps preserve structural integrity when the pollen grain is exposed to external environmental stresses. In addition, its viscous nature enables it to adhere to various floral and insect surfaces, facilitating the pollination process. To examine the physicochemical properties of aqueous pollenkitt droplets, we used in-line digital holographic microscopy to capture light scattering from individual pollenkitt particles. Comparison of pollenkitt holograms to those modeled using the Lorenz-Mie theory enables investigations into the minute variations in the refractive index and size resulting from changes in local temperature and pollen aging.

KEANEKARAGAMAN JENIS KUPU-KUPU DI KAWASAN AIR TERJUN TIGA BIDADARI KECAMATAN WASILE TIMUR KABUPATEN HALMAHERA TIMUR

Butterflies in nature provide benefits to the process of pollination and flower fertilization. Ecologically, it contributes to maintaining ecosystem balance and enriching biodiversity. The aim of this research is to identify the diversity of butterfly species in the Tiga Bidadari waterfall area and identify their food plants. The data collection method uses the Time Search method, namely an inventory method with observation plots not limited by a certain distance or area, but rather time (minutes). The data obtained will be analyzed using the Shannon-Wiener Diversity Index, relative abundance and dominance index. The research results showed that there were 15 species of butterflies ( 5 families ) namely, the Nymphalidae, Lycaenidae, Pieridae, Hesperiidae, and Papilionidae. The number of individuals is 90 individuals with a diversity index value of H' ꞊ 2.46 (medium diversity category). Based on the relative abundance of the species, Danaus genutia is the most common type of butterfly found in the Tiga Bidadari Waterfall area. A butterfly dominance index of 0.10 indicates that the butterfly species is not dominant. The food plants found consist of 12 types. The presence of butterflies in the Tiga Bidadari Waterfall area is influenced by food plants.

Modeling Positions and Orientations of Cantaloupe Flowers for Automatic Pollination

An automatic system for cantaloupe flower pollination in greenhouses is proposed to meet the requirements of automatic pollination. The system consists of a mobile platform, robotic manipulator, and camera that reaches the flowers to detect and recognise their external features. The main task of the vision system is to detect the position and orientation of the flower in Cartesian coordinates, allowing the manipulator to reach the pose and perform pollination. A comprehensive method to ensure the accuracy of the pollination process is proposed that accurately determines the position and orientation of cantaloupe flowers in real environments. The vision system is used to capture images, detect the flower, and recognise its state according to its external features, such as size, colour, and shape, thereby providing appropriate nozzle access during pollination. The proposed approach begins with a segmentation method designed to precisely locate and segment the target cantaloupe flowers. Subsequently, a mathematical model is used to determine the key points that are important for establishing the growth orientation of each flower. Finally, an inverse-projection method is employed to convert the position of the flower from a two-dimensional (2D) image into a three-dimensional (3D) space, providing the necessary position for the pollination robot. The experimental process is conducted in a laboratory and proves the efficacy of the cantaloupe flower segmentation method, yielding precision, recall, and F1 scores of 87.91%, 90.76%, and 89.31%, respectively. Furthermore, the accuracy of the growth-orientation prediction method reaches approximately 86.7%. Notably, positional errors in 3D space predominantly fall within the allowable range, resulting in a successful pollination rate of up to 83.1%.

Fixed non-random orientation to the Sun (conversotropism) in two window-flowered greenhood orchids, Diplodium spp. (Orchidaceae: Pterostylidinae); implications for other window flowers and pollinator behaviour

Abstract Many plant species orient their flowers to the Sun. Such orientations may be dynamic (heliotropism or solar tracking) or static, adopting fixed non-random azimuths to points on the daily arc of the Sun (conversotropism as defined herein). Two cauline greenhood orchid species, Diplodium reflexum and D. ampliatum (Pterostylidinae), are shown to be negatively conversotropic and non-heliotropic. Cauline greenhoods are trap and window flowers having large translucent light panels that glow when backlit flowers are viewed from the front. Conversotropism in greenhoods is thought to maximize light transmission through the window panels to manipulate the behaviour of fungus gnat pollinators. Conversotropism is so far documented in only seven species in five plant families, but is likely to be more widespread. The general characteristics of conversotropism are summarized and a new two stage model of window function in greenhoods is proposed. Window flowers in the Lady Slipper orchids (Cypripedium) and in Arums of the genus Arisaema are also predicted to be conversotropic. The evolution of conversotropism in cauline greenhoods highlights the importance of floral windows in these orchids. The highly unusual trap flowers may use light both as a pollinator attractant and repellent at different stages of the pollination process. Greenhoods appear to exploit the instinct of flies to escape confinement and the behaviours enabling it. This probably also applies to other trap flowers and adds insect escape behaviours to the many other drives, instincts, and behaviours exploited by plants for pollination.

Analysis of Pollination Process between Flowers and Honeybees to Derive Insights for the Design of Microrobots.

Pollination is a crucial ecological process with far-reaching impacts on natural and agricultural systems. Approximately 85% of flowering plants depend on animal pollinators for successful reproduction. Over 75% of global food crops rely on pollinators, making them indispensable for sustaining human populations. Wind, water, insects, birds, bats, mammals, amphibians, and mollusks accomplish the pollination process. The design features of flowers and pollinators in angiosperms make the pollination process functionally effective and efficient. In this paper, we analyze the design aspects of the honeybee-enabled flower pollination process using the axiomatic design methodology. We tabulate functional requirements (FRs) of flower and honeybee components and map them onto nature-chosen design parameters (DPs). We apply the "independence axiom" of the axiomatic design methodology to identify couplings and to evaluate if the features of a flower and a honeybee form a good design (i.e., uncoupled design) or an underperforming design (i.e., coupled design). We also apply the axiomatic design methodology's "information axiom" to assess the pollination process's robustness and reliability. Through this exploration, we observed that the pollination process is not only a good design but also a robust design. This approach to assessing whether nature's processes are good or bad designs can be valuable for biomimicry studies. This approach can also inform design considerations for bio-inspired innovations such as microrobots.

Integrative Resource Management in Multi Cloud Computing: A DRL Based Approach for multi-objective Optimization

INTRODUCTION: The multi-data canter architecture is being investigated as a significant development in meeting the increasing demands of modern applications and services. The study provides a toolset for creating and managing virtual machines (VMs) and physical hosts (PMs) in a virtualized cloud environment, as well as for simulating various scenarios based on real-world cloud usage trends.
 OBJECTIVES: To propose an optimized resource management model using the Enhanced Flower Pollination algorithm in a heterogeneous environment.
 METHODS: The combination of Q-learning with flower pollination raises the bar in resource allocation and job scheduling. The combination of these advanced methodologies enables our solution to handle complicated and dynamic scheduling settings quickly, making it suited for a wide range of practical applications. The algorithm finds the most promising option by using Q-values to drive the pollination process, enhancing efficiency and efficacy in discovering optimal solutions. An extensive testing using simulation on various datasets simulating real-world scenarios consistently demonstrates the suggested method's higher performance.
 RESULTS: In the end, the implementation is done on AWS clouds; the proposed methodology shows the excellent performance by improving energy efficiency, Co2 Reduction and cost having multi-cloud environment 
 CONCLUSION: The comprehensive results and evaluations of the proposed work demonstrate its effectiveness in achieving the desired goals. Through extensive experimentation on diverse datasets representing various real-world scenarios, the proposed work consistently outperforms existing state-of-the-art algorithms.