Objective: This study aims to investigate the impact of collective bargaining on the welfare of academic staff in federal universities located in South West Nigeria, focusing on six selected institutions. Theoretical Framework: The research is framed within the context of labour relations theory, exploring how collective bargaining as a mechanism influences various welfare dimensions of academic staff. Method: A quantitative research approach was adopted, utilizing a descriptive research design. The study's population comprised academic staff from the six universities, with a sample size of 377 participants determined through the Taro Yamane formula. Data were collected using structured questionnaires and analysed using statistical techniques to assess the relationship between collective bargaining and staff welfare. Results and Discussion: The results indicate a significant negative impact of collective bargaining on academic staff welfare (β=-0.494, p<0.05). Contrary to its purpose, collective bargaining has not adequately addressed welfare issues, potentially worsening existing challenges faced by academic staff in the studied universities. The discussion emphasizes the need for improved bargaining strategies to promote staff well-being. Research Implications: The study's findings highlight the importance of developing more effective and collaborative collective bargaining processes. These are critical for enhancing welfare outcomes and addressing systemic challenges within federal universities. Originality/Value: This research contributes to the literature on collective bargaining by providing empirical evidence from the Nigerian higher education sector, revealing its counterproductive effects on academic staff welfare. It offers practical insights for policymakers and unions in designing more strategic negotiation frameworks.