Problem In Postmenopausal Women Research Articles

The incidence of urogynaecological problems in postmenopausal women and its correlation with serum estradiol levels in a tertiary care hospital

The incidence of urogynaecological problems in postmenopausal women and its correlation with serum estradiol levels in a tertiary care hospital

Postmenopausal Vaginal Bleeding Due to Vaginal Leech Infestation: A Case Report

Introduction: Vaginal bleeding due to leech bites is a highly unusual and serious problem in postmenopausal women, potentially leading to severe complications and even death. Case Presentation: A 68-year-old rural woman, 13 years post-menopause, was admitted to a hospital in eastern Iran with a complaint of continuous vaginal bleeding with clot discharge for 12 days. During the medical examination, a dark worm moving in waves was observed in the labia minora of the vulva. The area was washed with 0.9% normal saline solution, and the leech was removed from the inner surface of the labia minora using forceps and pincers. The patient was monitored as an outpatient for 4 hours to control the bleeding and was subsequently discharged in good condition. She was advised to take iron tablets and folic acid for one month. Her test results were as follows: hemoglobin = 10 mg/dL, hematocrit = 32%, platelet count = 145,000, prothrombin time = 13 seconds, partial thromboplastin time = 35 seconds, and INR = 1.1. Her vital signs at admission were: temperature = 37.5°C, pulse rate = 100/min, blood pressure = 120/60 mmHg, and respiratory rate = 30 breaths per minute. Conclusions: Vulvar and vaginal leech bites can cause severe vaginal bleeding and anemia in postmenopausal women. To reduce complications caused by delays in diagnosis or treatment, healthcare workers should consider the possibility of vulvar and vaginal leech bites in postmenopausal rural women and individuals who use river water for drinking, bathing, or showering. Taking a detailed history and performing a thorough physical examination before any diagnostic procedure can help quickly and accurately diagnose the cause of bleeding in these patients.

Sleep, Mood, and Nutrition Patterns of Postmenopausal Women Diagnosed with Major Depressive Disorder by Menopause Periods.

Menopausal women experience changes in ovarian activity and fluctuating hormone levels. The aim of this study was to detect ongoing sleep and nutritional problems in postmenopausal women. This study was conducted with 62 postmenopausal women who had been diagnosed with major depressive disorder, were aged 42-64, were not dieting for at least 1 month, and had been using antidepressants for at least 6 months. The Pittsburgh Sleep Quality Index and Depression Anxiety Stress Scale-42 were used. Anthropometric measurements were taken and body composition analysis was performed. The prevalence of obesity and overweight were 12.9% and 71%, respectively. Hot flashes, night sweats, and mood swing were more common in those who had been through menopause for <5 years. Also, the PSQI, Depression, and Stress scores of those women were higher. Women who had been menopausal for ≥5 years had a higher BMI, waist/hip ratio, and fat mass and better sleep quality and lower daytime dysfunction according to the PSQI. Energy and fat intake were higher, while protein, vitamin C, and calcium intakes were lower in women who had been menopausal for ≥5 years. It is crucial for healthcare professionals to review approaches for early and late menopausal periods and to individualize treatment options, especially in patients whose symptoms persist.

Prevalence and associated factors of depression in postmenopausal women: a systematic review and meta-analysis

BackgroundDepression is a prevalent mental health problem in postmenopausal women. Given its significant impact on the quality of life and overall well-being of postmenopausal women, there is need for a comprehensive review and meta-analysis of the existing research globally. This systematic review and meta-analysis evaluated the global prevalence of depression and potential associated factors in postmenopausal women.MethodsThe Cochrane Library, PubMed, EMBASE, Web of Science, MEDLINE, and PsycINFO databases were systematically searched from inception to March 22, 2023. The meta-analysis used the random-effects model to calculate the prevalence of depression rates and associated factors. In addition, subgroup analysis and sensitivity analysis were performed. Publication bias was assessed using funnel plots, Egger’s test, and nonparametric trim-and-fill tests.ResultsThe meta-analysis included 50 studies that involved 385,092 postmenopausal women. The prevalence of depression in postmenopausal women was 28.00% (95% CI, 25.80–30.10). Among the factors relevant to depression among postmenopausal women, marital status (OR: 2.03, 95%CI: 1.33–3.11), history of mental illness (OR: 2.31, 95%CI: 1.50–3.57), chronic disease (OR: 3.13, 95%CI: 2.20–4.44), menstrual cycle (OR: 1.42, 95%CI: 1.17–1.72), abortion numbers (OR: 1.59, 95%CI: 1.40–1.80), menopausal symptoms (OR: 2.10, 95%CI: 1.52–2.90), and hormone replacement therapy (OR: 1.76, 95%CI: 1.31–2.35) were risk factors, while physical activity (OR: 0.56, 95%CI: 0.53–0.59), number of breastfed infants (OR: 0.43, 95%CI: 0.19–0.97), menopause age (OR: 0.44, 95%CI: 0.37–0.51) were preventive factors.ConclusionsThis study demonstrated that the prevalence of postmenopausal depression is high, and some risk factors and protective factors associated with it have been identified. It is necessary to improve screening and management and optimize prevention and intervention strategies to reduce the harmful effects of postmenopausal depression.

URINARY TRACT INFECTION (UTI) IN POSTMENOPAUSAL WOMEN WITH TYPE 2 DIABETES MELLITUS USING SODIUM GLUCOSE CO TRANSPORTER 2 INHIBITORS (SGLT-2 I)

OBJECTIVE. To determine the frequency of urinary tract infection in postmenopausal women with type 2 diabetes mellitus using sodium-glucose co-transporter 2 inhibitors. MATERIAL AND METHODS. This descriptive cross-sectional study was performed on 101 postmenopausal patients with type 2 diabetes mellitus using sodium-glucose co-transporter 2 inhibitors. Samples of blood and urine were sent to the hospital laboratory for analysis. Data was analyzed using SPSS version 20. The Chi-square test was performed after stratification. RESULTS. The mean and standard deviation for age was 60.9±4.4. 24.8% of post-menopausal women with type 2 diabetes mellitus using sodium-glucose co-transporter 2 inhibitors were having urinary tract infections. The majority of these patients were illiterate or had primary education and the duration of diabetes was more than 10 years. CONCLUSION. Urinary tract infection is a common problem in postmenopausal women with type 2 diabetes mellitus using sodium-glucose co-transporter 2 inhibitors. Patients with poorly controlled diabetes and increased duration of menopause have an increased risk of urinary tract infection. KEYWORDS. Urinary tract infection (UTI), Post-menopausal, type 2 diabetes mellitus (DM), sodium-glucose co-transporter 2 inhibitors (SGLT2Is).

Type 2 Diabetes Mellitus Prevalence and Associated Risk Factors in Postmenopausal Women.

Introduction Type 2 diabetes mellitus (T2DM) is the most common problem in postmenopausal women. This study aimed to find out the prevalence of T2DM and its risk factors in postmenopausal women. Methods The study is a population-based cross-sectional study. Anthropometric measurements, blood pressure, and biochemical measurements of 2295 postmenopausal women up to the age of 55 years were taken following face-to-face interviews. Odds ratio was used to find out the role of risk factors associated with T2DM. Results Prevalence of T2DM was reported to be 15.51%. Significant contribution of waist circumference (WC) followed by body mass index (BMI), total cholesterol (TC), and triglycerides (p<0.05) was noted in increasing the risk of T2DM. No association was found between T2DM and hypertension (p>0.05). Conclusion A high prevalence of T2DM was reported in postmenopausal women. Higher levels of BMI, WC, TC, and triglycerides were found to be the major risk factors for T2DM.

Postmenopausal women's usage of complementary and alternative medicine and its relationship to sexual function: A cross-sectional study in southeastern Iran.

Sexual dysfunction is one of the most common problems in postmenopausal women that affect their lives. Due to the widespread disadvantages and age limit of drug and hormone therapy, the demand for complementary and alternative medicine (CAM) methods has increased. This was a descriptive-analytical study. A total of 297 postmenopausal women who were referred to health centers in Kerman, southeastern Iran, were selected during the years 2020-2021. The use of CAM and sexual function were examined by Complementary and Alternative Medicine Questionnaire and the Female Sexual Function Index, respectively. Data were collected through a combination of face-to-face and online forms. More than half of the participants used at least one type of CAM in the preceding year for any general reason. The mean satisfaction score for CAM use was 21.7 ± 5.84; Most reasons of use were for improving quality of life, improving physical function, reducing sleep disorders, improving mood status, reducing vasomotor symptom, and improving sexual function, respectively. Most reasons of use were related to sexual dysfunction. Specifically, the mean score for sexual function was 17.96 ± 7.50 which was lower than the scale's midpoint of 28. According to different subscales of sexual function, 52.9% of participants had good desire, 49.5% good arousal, 34.7% good lubrication, 56.9% good orgasm, and 2% had no pain, and 52.5% were satisfied with their sexual function. There were no significant differences between CAM users and nonusers about sexual functions and all its subscales. There were no significant differences between CAM users and nonusers about sexual functions and all its domains. Further research in different communities with different health systems is recommended to investigate the relationship between CAM and sexual function among postmenopausal women.

11β-HYDROXYSTEROID DEHYDROGENASE TYPE 1 KNOCK-OFF PROTECTS AGAINST OVARIECTOMY-INDUCED OSTEOPOROSIS BY INHIBITING OSTEOCLAST ACTIVATION

Osteoporosis is a common problem in postmenopausal women and the elderly. 11β-Hydroxysteroid dehydrogenase type 1 (11β-HSD1) is a bi-directional enzyme that primarily activates glucocorticoids (GCs) in vivo, which is a considerable potential target as treatment for osteoporosis. Previous studies have demonstrated its effect on osteogenesis, and our study aimed to demonstrate its effect on osteoclast activation.In vivo, we used 11β-HSD1 knock-off (KO) and C57BL6/J mice to undergo the ovariectomy-induced osteoporosis (OVX). In vitro, In vivo, We used 11β-HSD1 knockoff (KO) and C57BL6/J mice to undergo the ovariectomy-induced osteoporosis (OVX). In vitro, bone marrow-derived macrophages (BMM) and bone marrow mesenchymal stem cell (BMSC) of KO and C57BL6/J mice were extracted to test their osteogenic and osteoclastic abilities. We then created osteoclastic 11β-HSD1 elimination mice (Ctsk::11β-HSD1fl/fl) and treated them with OVX. Micro-CT analysis, H&amp;E, immunofluorescence staining, and qPCR were performed. Finally, we conducted the high-throughput sequencing to find out 11β-HSD1 and osteoclast activation related genes.We collected 6w samples after modeling. We found that KO mice were resistant to loss of bone trabeculae. The same effect was observed in osteoclastic 11β-HSD1 elimination mice. Meanwhile, BVT-2733, a classic inhibitor of 11β-HSD1, inhibited the osteoclast effect of cells without affecting osteogenic effect in vitro. High-throughput sequencing suggested that glucocorticoid receptor (GR) may play a key role in the activation of osteoclasts, which was verified by immunofluorescence staining and WB in vivo and in vitro.In the process of osteoporosis, 11β-HSD1 expression of osteoclasts is abnormally increased, which may be a new target for inhibiting osteoclast activation and treating osteoporosis.

Effect of Vitamin E Supplementation on Chronic Insomnia Disorder in Postmenopausal Women: A Prospective, Double-Blinded Randomized Controlled Trial.

Chronic insomnia disorder is one of the most common problems in postmenopausal women, exacerbated by underdiagnosis and improper treatment. This double-blinded, randomized, placebo-controlled trial was conducted to evaluate the potential of vitamin E to treat chronic insomnia as an alternative to sedative drugs and hormonal therapy. The study enrolled 160 postmenopausal women with chronic insomnia disorder, divided randomly into two groups. The vitamin E group received 400 units of mixed tocopherol daily, while the placebo group received an identical oral capsule. The primary outcome of this study was sleep quality assessed by the Pittsburgh Sleep Quality Index (PSQI), a self-evaluated and standardized questionnaire. The secondary outcome was the percentage of participants using sedative drugs. There were no significant differences in baseline characteristics between the study groups. However, the median PSQI score at baseline was slightly higher in the vitamin E group compared with the placebo (13 (6, 20) vs. 11 (6, 20); p-value 0.019). After one month of intervention, the PSQI score was significantly lower (indicating better sleep quality) in the vitamin E group compared with the placebo (6 (1, 18) vs. 9 (1, 19); p-value 0.012). Moreover, the improvement score was significantly higher in the vitamin E group compared with the placebo (5 (-6, 14) vs. 1 (-5,13); p-value < 0.001). In addition, there was a significant reduction in the percentage of patients using sedative drugs in the vitamin E group (15%; p-value 0.009), while this reduction was not statistically significant in the placebo group (7.5%; p-value 0.077). This study demonstrates vitamin E's potential as an excellent alternative treatment for chronic insomnia disorder that improves sleep quality and reduces sedative drug use.

Effects of a calcium/vitamin D/Zinc combination on anti-osteoporosis in ovariectomized rats

BackgroundOsteoporosis is a major health problem in postmenopausal women, and characterized by deteriorated bone mass and micro-architecture. There have been some clinical trials demonstrating the beneficial effects of vitamin-D and some trace elements on calcium absorption and attenuation of osteoporosis development. However, effects of the combination of vitamin-D and zinc on calcium absorption and osteoporosis have not been adequately investigated. MethodsNetwork pharmacology was first performed to explore possible correlations between calcium/vitamin D/zinc and osteoporosis. Forty-nine female Sprague-Dawley rats (6 months old, 250 ± 20 g) were randomized into 7 experimental groups with 7 animals per group for the in vivo study, including one sham surgery control group, one ovariectomizing (OVX) group, and 5 OVX plus treatment groups. At the end of animal experiment, animal tibia and femur leg bones and blood were collected for H&E staining, bone microstructure analysis by a micro-CT, measurement of bone and serum Ca, P and Zn concentrations, and immunohistochemical detection of macrophage-colony stimulating factor receptor (M-CSFR) and receptor activator of nuclear factor-kappa B ligand (RANKL). ResultsThe network pharmacology analysis identified 57 candidate targets that were related to the osteoporosis-Ca/VitD/Zn interconnections. Further pathway analysis suggested that the combined treatment of Ca, VitD and Zn attenuated osteoporosis via modulation of metabolic pathways. We found that a therapy with Ca/VitD-M/Zn-M (73 mg/kg/day Ca, 0.6 g/kg/day VitD3 and 0.6 mg/kg/day zinc citrate) could significantly suppress the progression of osteoporosis in rats. After the Ca/VitD-M/Zn-M treatment, the ratio of bone volume/tissue volume, trabecular number and the trabecular thickness were all significantly elevated while the extent of trabecular separation was significantly reduced. Additionally, both serum calcium and bone calcium levels were significantly upregulated by the Ca/VitD/Zn treatment in a dose-dependent manner. The combination of Ca/VitD-M/Zn-M was superiou to either Ca/VitD-L/Zn-L or Ca/VitD-H/Zn-H treatment for such an effect. Moreover, the osteoporosis-associated M-CSFR and RANKL factors were both significantly downregulated by the Ca/VitD-M/Zn-M treatment in bone tissues of OVX rats. ConclusionsThe combined supplement of VitD and Zn facilitates the Ca(2 +) absorption and attenuates the development of osteoporosis via down-regulation of osteoporosis-associated factors M-CSFR and RANKL, thus potentially constitutes an alternative therapy for the postmenopausal osteoporosis.

Epithelial thinning in vaginal atrophy related to lowering of calcitonin gene-related protein, vascular endothelial growth factor, and nerve growth factor expressions in a menopausal rat model

Context: Vaginal atrophy has been observed as a common sexual problem in post-menopausal women. The targeted protein to counteract menopause problems related to vaginal epithelial thinning is currently a research problem that has not been fully investigated. Aims: To explore the possible mechanism underlying vaginal atrophy in rat models. Methods: Following three-week ovariectomy (OVX), Sprague-Dawley female rats were randomly divided into two groups and orally administered estradiol for two weeks in the treated group. In parallel with this, six rats with sham surgery were used as control. Marker-related vaginal atrophy, including calcitonin gene-related protein (CGRP), vascular endothelial growth factor (VEGF), and nerve growth factor (NGF) in the vaginal wall, were compared using immunohistochemistry. Results: OVX as a menopausal model significantly induced vaginal epithelial cell thinning and decreased the expression of CGRP, VEGF, and NGF compared with sham surgery animals (p&lt;0.05). Estrogen replacement in OVX rats reversed the vaginal atrophic by recovering the protein expression CGRP, VEGF, and NGF (p&lt;0.05). Conclusions: Thus, it may be concluded that a possible mechanism underlying the OVX-induced vaginal atrophy may be related to the downregulation expression of CGRP, VEGF, and NGF in vaginal tissue.

Ekstrak Etanol Ubi Jalar Ungu untuk Mencegah Peningkatan pH Vagina setelah Ovariektomi Bilateral pada Tikus Wistar

AVaginal atrophy is one of the most common health problems in postmenopausal women. The disease is characterized by decreasing layers and maturation index of the vaginal epithelium and an increase in vaginal pH. A decrease in vaginal acidity will increase the susceptibility to infections since acidic pH protects the vagina from pathogens infection. The previous study has proven that phytoestrogens were effective to improve the microstructures of the vaginal, reduce urogenital complaints, and improve vaginal pH. The purple sweet potato (Ipomea batatas L.) which is cultivated in Bali was recognized for its high anthocyanin content and was reported to have phytoestrogen activity. The aim of the present study is to evaluate the effect of purple sweet potato on vaginal pH in an animal model of menopause. The randomized pre-test post-test control group design study was conducted using 36 bilateral ovariectomized female Wistar rats as an animal model of menopause. The animals were divided randomly into a control group (T0) which was given saline and a treatment group (T1) given an ethanolic extract of purple sweet potato 400 mg/day for 30 days. Vaginal pH was measured before treatment, every week during treatment, and after treatment. The result of the study showed that there was an increase in vaginal pH of the T0 on the 2nd and 3rd week, and after treatment (P

Correlation between Vitamin D and Uric Acid in Menopausal Women

Background : Vitamin D deficiency and hyperuricemia are global health problems in post-menopausal women in which parathyroid hormone increases hyperuricemia incidence.&#x0D; Aim : This research purpose to determine correlation between vitamin D and uric acid levels in postmenopausal women&#x0D; Methods: This research is an observational analytic study with a cross-sectional study design conducted at University of North Sumatra Hospital and Network Hospital in Medan for 12 months on 40 postmenopausal women who met inclusion and exclusion criteria. The data will be analyzed descriptively to determine variables frequency distribution. Previously, normality test of numerical data is carried out. If the data is normally distributed, Pearson correlation test will be carried out, and if the data is not normally distributed, then Spearman correlation test will be used.&#x0D; Results : Mean vitamin D of the sample was 23.11 ng/mL with a standard deviation 4.43 and median value 23.5 ng/mL. while the mean uric acid was 7.52 mg/dL with a standard deviation 0.92 and median value 7.45 mg/dL. There is an inverse relationship between uric acid levels and Vitamin D, as evidenced by Pearson test correlation coefficient of -0.541 with p value = 0.001.&#x0D; Conclusion : There is an inverse correlation between Vitamin D and uric acid levels.

The volume of brisk walking is the key determinant of BMD improvement in premenopausal women.

SummaryOsteoporosis is an increasing health problem in postmenopausal women. Our findings indicated that long-term brisk walking with a volume greater than 16 per week is effective for improving BMD in premenopausal women.PurposeTo examine the effects of brisk walking on bone mineral density (BMD) in premenopausal women, and further determine the effective frequency, intensity, time and volume (frequency x duration) of brisk walking for training strategy prescription.Methods222 healthy premenopausal women were recruited for BMD measurement. According to the survey of their physical activity level, 84 subjects (age: 46±1.8) whose physical activity index ≥40 were categorized into the brisk walking group, and 138 subjects (age: 47±2.2) whose physical activity index <40 were assigned to the sedentary group. The BMD of these two groups were statistically compared with an independent t test. Next, 35 subjects from the original sedentary group were recruited for BMD measurement after 2-year moderate brisk walking. According to the volume of physical activity per week, they were divided into the control group (n = 10, aged 49±0.9), volume 8 group (n = 4, aged 48±1.2), volume 12 group (n = 7, aged 49±1.4), volume 16 group (n = 8, aged 49±1.3), and volume 20 group (n = 6, aged 49±1.5). ANOVA was used to analyze BMD before and after brisk walking among the five groups.ResultsThe BMD in the brisk walking group (1.00±0.008 g/cm2) was significantly higher than that in the sedentary group (0.89±0.008 g/cm2) (P<0.001). Stepwise regression analysis revealed that the volume of brisk walking was significantly correlated with BMD (P<0.001). In particular, brisk walking with a volume greater than 16 (a score of duration up to 4 and a score of frequency up to 4 or 5) per week is effective for improving BMD in premenopausal women (P = 0.03, P = 0.002, respectively).ConclusionsLong-term brisk walking is an efficient way to improve BMD. Taking brisk walks for 30 minutes per day 3 or more times per week (volume>16) is recommended to prevent bone loss in premenopausal women.

Impact of Physical Activity on Physical and Mental Health of Postmenopausal Women: A Systematic Review

Introduction: Menopause is associated with a variety of physical and mental issues. It’s critical to pay attention to postmenopausal women’s health issues and develop prevention strategies. Aim: To evaluate association between physical activity and physical and mental health problems in postmenopausal women. Materials and Methods: In the present systematic review, relevant studies were searched in international electronic databases such as, Cochrane CENTRAL, PubMed and Google Scholar from 2004 to 2021. Terms like physical activity, physical health, mental health, bone mineral density, exercise training, and postmenopausal women were searched. All papers were screened for eligibility. Inclusion criteria of the study was, studies with atleast one exercise group versus one control group, should include women who were postmenopausal at the time of the study, studies that examined effect of physical activity on physical and mental health of postmenopausal women, full text articles written in English language. Results: Total 27 studies were included in this review. The results revealed that resistance, aerobics, walking, pilates and aquatic exercises have shown benefits in improving physical and mental health parameters of postmenopausal women. Conclusion: Physical activity plays a great role in maintaining overall health and quality of life of women. To avoid fractures, these women should engage in workouts with prudence. Physical and mental fitness can be achieved by incorporating exercise into daily routine of postmenopausal women.

THE MOST COMMON UROLOGICAL CONDITIONS IN POSTMENOPAUSAL WOMEN.

The aim: To analyze the available literature on the most common daily urological problems in menopausal women and to evaluate the use of hormone replacement therapy for troublesome urological symptoms. Materials and methods: Analysis of publications from PubMed databases on the most common disorders during menopause was performed and the most common urog¬ynaecological problems in postmenopausal women were selected according to literature data. Different available methods of treatment of these disorders were compared. Conclusions: During menopause, women struggle with many unpleasant symptoms from the genitourinary system. For most women, this is a very embarrassing topic and, although bothersome, underestimated. The urinary tract infections, urinary incontinence or kidney stones can lead to serious complications, if left untreated. We should strive to make women more aware of possible methods of prevention and treatment of the menopausal symptoms in the context of urological disorders.

Tocotrienol Supplementation Led to Higher Serum Levels of Lysophospholipids but Lower Acylcarnitines in Postmenopausal Women: A Randomized Double-Blinded Placebo-Controlled Clinical Trial.

Osteoporosis is a major health problem in postmenopausal women. Herein we evaluated the effects of 12-week tocotrienols (TT) supplementation on serum metabolites in postmenopausal, osteopenic women. Eighty-nine participants (59.7 ± 6.8 yr, BMI 28.7 ± 5.7 kg/m2) were assigned to 3 treatments: placebo (860 mg olive oil/day), 300mg TT (300 mg TT/day), and 600mg TT (600 mg TT/day) for 12 weeks. TT consisted of 90% δ-TT and 10% γ-TT. In this metabolomic study, we evaluated the placebo and 600mgTT at baseline and 12 weeks. As expected, TT and its metabolite levels were higher in the supplemented group after 12 weeks. At baseline, there were no differences in demographic parameters or comprehensive metabolic panels (CMP). Metabolomics analysis of serum samples revealed that 48 biochemicals were higher and 65 were lower in the 600mg TT group at 12 weeks, compared to baseline. The results confirmed higher serum levels of tocotrienols and lysophospholipids, but lower acylcarnitines and catabolites of tryptophan and steroids in subjects given 600mg TT. In summary, 12-week TT supplementation altered many serum metabolite levels in postmenopausal women. The present study supports our previous findings that TT supplementation helps reduce bone loss in postmenopausal osteopenic women by suppressing inflammation and oxidative stress. Furthermore, the body incorporates TT which restructures biomembranes and modifies phospholipid metabolism, a response potentially linked to reduced inflammation and oxidative stress.

In Silico Antiosteoporosis Activity of 96% Ethanol Extract of Chrysophyllum cainito L. Leaves

Estrogen deficiency causes various health problems in postmenopausal women, including osteoporosis. Phytoestrogens are emerging as potential estrogen alternatives with minimal side effects. This study aimed to predict the antiosteoporosis activity of the compounds from 96% ethanol extract of Chyrsophyllum cainito L. leaves through in silico study on 3OLS protein, an X-ray protein of ERβ. In silico analysis was carried out on the compounds from metabolite profiling results of 96% ethanol extract of C. cainito leaves from previous studies. The structure of compounds resulting from metabolite profiling of 96% ethanol extract of C. cainito leaves was made using Avogadro 1.0.1 software, geometry optimization with Chemdraw molecular docking was carried out using PyRx 0.8 software, and Biovia Discovery Studio Visualizer 2021 software was used to visualize the structure of compounds against 3OLS proteins. The physicochemical characteristics of the compounds were analyzed using the SwissADME webtool. From the result, it was known that there were seven compounds in the leaves of C. cainito, which were suspected to be phytoestrogens that had ERβ agonist properties against 3OLS protein. It was an ERβ agonist because the compound had similar parameters to 17β-estradiol in its interaction with the 3OLS protein, which had a pharmacophore distance of about 10,862, and bound to the amino acids His 475 and Glu 305 or Arg 346. The 96% ethanol extract of C. cainito leaves contained seven compounds thought to be phytoestrogen with an ERβ agonist that handled its antiosteoporosis activity.

Effects of lifestyle on sexual function among postmenopausal women

A healthy lifestyle has a key role in reducing health problems. Since one of the most common problems in Postmenopausal women has been sexual dysfunction (SD). The specific purpose of the present study was to identify the effects of health-promoting lifestyle (HPL) on sexual function among postmenopausal women. The present cross-sectional, descriptive, and analytical study was conducted on 405 Postmenopausal women aged 45-60 years, using the convenience sampling method.Data collection was done using three questionnaires of demographic, health-promoting lifestyle profile-II (HPLP-II) and female sexual function index (FSFI). Data were analyzed in the SPSS-16 using Pearson's correlation coefficient. The statistical significance level was regarded as less than 0.05. In general, the result of this study identified a 68% prevalence of SD among participants. The mean score obtained from the HPLP II was 2.27 (SD = 0.42), the highest score of its sub-scales was spiritual growth and the lowest score was physical activity.The mean score of FSFI among the studied women was 23.16 (SD = 0.29), the highest score of six sub-scales was satisfaction and the lowest score was lubrication among participants. A strong correlation was found between the total FSFI scores, and spiritual growth (r=0.048), interpersonal relations (r=0.02), stress management (r=0.000), (p<0.0001). The results of the study revealed that a healthy lifestyle affects sexual function. Given that a healthy lifestyle is one of the most important ways to help women overcome SD, a healthy lifestyle promoting interventions necessary for Postmenopausal women.

Prediction of compounds with antiosteoporosis activity in Chrysophyllum cainito L. leaves through in silico approach.

Estrogen deficiency causes various health problems in postmenopausal women, including osteoporosis. Phytoestrogen emerged as a potential alternative of estrogen with minimum side effects. The aims of this study were to analyze the metabolite profiling results of various extract of Chyrsophyllum cainito L. leaves, which contain phytoestrogen, through in silico study against 3OLS protein, an X-ray protein of ERβ, so it can predict the types of the phytoestrogen contents which have antiosteoporosis property. In silico analysis was carried out for the compounds from the metabolite profiling data of C.cainito leaves from our previous study. The structure compounds from metabolite profiling results of various extract of C.cainito leaves were prepared with Avogadro 1.0.1 software, molecular docking was done using PyRx 0.8 software, and Biovia Discovery Studio Visualizer 2016 software was used to visualize the structure of compounds against 3OLS protein. The physicochemical characteristics of the compounds were analyzed using the SwissADME web tool. From in silico studies, it was known that there were total 11 compounds in C.cainito leaves that predicted as phytoestrogens which have ERβ agonist properties against 3OLS protein. The ERβ agonist was a compound that has parameters similar to 17β-estradiol in its interaction with 3OLS protein, which has a pharmacophore distance of 10.862Å, and binding to amino acids His 475 and Glu 305 or Arg 346at receptor-ligand docking simulation. C.cainito leaves contain 11 compounds that are predicted to be phytoestrogens with ERβ agonist properties, which is responsible for antiosteoporosis activity.