Using software in mathematics learning can improve students' soft and hard mathematics skills at the high school and college levels. Therefore, using software in the learning process is important, including in learning Differential Equations. This research examines the use of the Python programming language with Jupyter Lab software and the SymPy library in solving ordinary differential equation problems symbolically in the Differential Equations course. The use of the Python programming language in Differential Equations learning includes solving linear ordinary differential equations of first-order, second-order, higher-order, and the Laplace transform. This research also examines the effect of using Python on the learning outcomes of differential equations of Mathematics Education Study Program students, Study Program Outside the Main Campus, Pattimura University. The population in this quantitative research is all students who programmed differential equations courses in the even semester of the 2023-2024 academic year as many as 19 students. The Python programming language can be used to solve differential equation problems symbolically easily, quickly, and accurately. In addition, using Jupyter Lab makes the process of solving differential equation problems easier and more interactive. Furthermore, t-test results show that the use of Python in learning differential equations can improve students' learning activities and learning outcomes. Using the Python programming language with Jupyter Lab software and the SymPy library can be developed to create teaching materials, textbooks, and reference books for Differential Equations courses.