Allergic asthma is a chronic inflammatory disorder characterized by immune system changes. Allergen inhalation, which causes hyperactivity, eosinophil recruitment, mast cells, and lymphocytes in the upper and lower respiratory tracts, triggers the inflammation cascade and results in local and systemic inflammation responses. However, the mechanism of probiotics in allergic diseases, both in vivo and in vitro, is not fully understood. This research was conducted to observe the effect of single or combined probiotics on the immune system of Sprague Dawley rats. This research used a pre-post-test only control group design. The research was conducted on 30 Sprague Dawley rat models with chronic asthma induced with ovalbumin, divided into 5 groups (NC: negative control; PC: positive control; Lm: Leuconostoc mesenteriodes; Lb: Lactobacillus brevis; Lm+Lb: a combination of Leuconostoc mesenteriodes and Lactobacillus brevis). The examinations on eosinophil and neutrophil and IL 17, IL 10, and IL 4 were conducted using the ELISA method. The data were statistically tested using IBM SPSS 25. The administration of single or combined probiotics increased IL-10 (p < 0.001) yet decreased IL-17 and IL-4 (p < 0.001) in all treatment groups. The results show that using single probiotics, Leuconostoc mesenterium best increased IL-10 and decreased IL-17 and IL-4. The dosage administration of single or combined probiotics had a systemic effect on overcoming allergic asthma. In this research, the administration of single probiotics had a better effect when compared with that of combined probiotics.