Probe For Detection Of H2S Research Articles

Construction of a highly specific fluorescence “turn-on” probe for H2S detection and imaging in drug-induced live cells, zebrafish and mice arthritis models

Quantitatively and selectively detecting the biomarker of hydrogen sulfide (H2S) in arthritis diseases is of great significance for the early diagnosis and treatment of arthritis. Modern medical studies show that H2S as a biomarker is involved in the development of inflammation. In this work, a new highly specific fluorescence “turn-on” probe JMD-H2S was tailored for H2S detection and imaging in drug-induced live cells, zebrafish and mice arthritis models, which utilized pyrazoline molecule as the fluorescence signal reporter group and 2,4-dinitrophenyl ether group (DNB) with strong intramolecular charge transfer (ICT) effect as the H2S recognition moiety and fluorescence quenching group. JMD-H2S showed a fast response time (<60 s), a large fluorescence response ratio (enhanced ∼20 folds) at I453/I0, excellent sensitivity toward H2S over other analytes, and an outstanding limit of detection (LOD) as low as 25.3 nM. In addition, JMD-H2S has been successfully applied for detecting and imaging H2S in drug-induced live cells, zebrafish, and mice arthritis models with satisfactory results, suggesting it can be used as a robust molecular tool for investigating the occurrence and development of H2S and arthritis.

A hemicyanidin-based NIR fluorescent probe for detection of H2S and imaging study in cells and mice.

The selective detection of hydrogen sulfide in physiological and pathological processes has gained substantial attention in recent years. However, the real-time detection of hydrogen sulfide remains an elusive goal. In this work, a new type of hemicyanidin-based fluorescent "turn-on" probe NTR-HS (Ex = 680nm, Em = 760nm) was developed to detected H2S in a very short time (3min).Thefluorescence quantum yield is 0.15 and accompanied with a noticeable color change from violet to bluethatcan be used to detect H2S in the range 1.04 × 10-7-4 × 10-5Mwith a limit of detection of 1.04 x 10-7 M. TheNTR-HS probewas also used for imaging of endogenous hydrogen sulfide and mitochondrial localization in HCT116 and HeLa cells. The detection mechanism was studied through fluorescence, UV-Vis, NMR, and mass analysis. Notably, the probe was successfully used to imaging H2S in mice and locating hydrogen sulfide in the large intestine of mice.

A dual-responsive fluorescent probe for detection of H2S and Cu2+ based on rhodamine-naphthalimide and cell imaging

A highly selective and sensitive fluorescence probe (DTR-1) for simultaneously detecting H2S and Cu2+ with different fluorescence signals was synthesized. DTR-1 displayed remarkable fluorescence enhancement (560 nm, 34-fold) when H2S was added under excitation of 410 nm with the solution’s color change from dark to green. The limit of detection (LOD) for H2S was calculated to be 0.035 µM. By contrast, the addition of Cu2+ enhanced obviously the emission intensity of DTR-1 with the maximal peak at 630 nm and color change from colorless to orange. LOD for Cu2+ was determined to be 0.16 µM. The sensing mechanism for H2S and Cu2+ in DMF/PBS (9/1, v/v, pH = 7.4) was studied by 1H NMR and ESI-MS. Furthermore, the potential application of DTR-1 in biological systems had been demonstrated through imaging of living cells.

A Quinolinium-Phenol Vinylic Conjugated Fluorescent Probe for H2s Detection Based on H2s-Triggered Release of Protected Group

A Quinolinium-Phenol Vinylic Conjugated Fluorescent Probe for H2s Detection Based on H2s-Triggered Release of Protected Group

Perylene diimide dye threaded with dual-react able sites for detection of H2S and Hg2+: Diagnostic kit and cell imaging

Designing of chemodosimetric probe for detection of H2S and Hg2+ at nanomolar level is highly important and challenging because of their toxicity to living organisms and the environment. Herein, we report unique chemodosimeteric design approach, where perylene diimide core is threaded by dual-react able sites for selective detection of H2S and Hg2+ in water. PDI 3 retained its chromophoric properties in solution, solid state and could be used to develop solution/solid state-based diagnostic kits for on-site detection of certain concentrations of H2S (0.034–0.5 ppm). PDI 3 showed ultra-sensitivity for H2S (UV–vis = 4.6 nM and Fluorescence = 19.5 nM) and Hg2+ (UV–vis = 60 nM and Fluorescence = 33 nM) in solution and 140 pg/cm2 and 19.9 pg/cm2 respectively using TLC strip (solid state). Kinetic profiles of PDI 3 showed the completion of the reaction within 14 min with pseudo-first order rate constant (k = 5.13 × 10−1/min). PDI 3 has good permeability, low cytotoxicity and was successfully applied for H2S and Hg2+ bio-imaging in MG-63 cells and for determination of H2S in human serum and urine samples.

A Fast-Response Red Shifted Fluorescent Probe for Detection of H2S in Living Cells.

Near-infrared (NIR) fluorescent probes are attractive tools for bioimaging applications because of their low auto-fluorescence interference, minimal damage to living samples, and deep tissue penetration. H2S is a gaseous signaling molecule that is involved in redox homeostasis and numerous biological processes in vivo. To this end, we have developed a new red shifted fluorescent probe 1 to detect physiological H2S in live cells. The probe 1 is based on a rhodamine derivative as the red shifted fluorophore and the thiolysis of 7-nitro 1,2,3-benzoxadiazole (NBD) amine as the H2S receptor. The probe 1 displays fast fluorescent enhancement at 660 nm (about 10-fold turn-ons, k2 = 29.8 M−1s−1) after reacting with H2S in buffer (pH 7.4), and the fluorescence quantum yield of the activated red shifted product can reach 0.29. The probe 1 also exhibits high selectivity and sensitivity towards H2S. Moreover, 1 is cell-membrane-permeable and mitochondria-targeting, and can be used for imaging of endogenous H2S in living cells. We believe that this red shifted fluorescent probe can be a useful tool for studies of H2S biology.

A Seminaphthorhodafluor-Based Fluorescent Probe for H2S Detection and Its Application

A Seminaphthorhodafluor-Based Fluorescent Probe for H₂S Detection and Its Application

Instantaneous fluorescent probe for the specific detection of H2S

Novel cyanine-based fluorescent probes for the detection of H2S were developed. The probes developed are stable under physiological conditions. The water soluble fluorescent probe 2 displayed ultrafast and specific response to H2S displaying NIR fluorescence of 115-fold turn-on with the detection limit of 11 nM without assistance of organic solvent or surfactant. Cell imaging experiments indicated that probe 2 was cell-permeable and was able to detect H2S sensitively in lysosomes. Moreover, our probe was able to detect H2S intrinsically produced H2S through enzymatic/non-enzymatic biosynthetic pathway from Cys/GSH. Moreover, we applied probe 2 to detect H2S in living mice and demonstrated the fast metabolism of H2S. Thus, probe 2 shows great promise as a reporter for H2S.

Metal-organic framework nanosheets with flower-like structure as probes for H2S detection and in situ singlet-oxygen production.

Herein, a novel bifunctional flower-like metal-organic framework (MOF) was designed as a probe, which could be activated by H2S, induce itself disassembly and then release linker, Zn porphyrin. Therefore, the fluorescence of the system is enhanced and 1O2 could be produced in situ, opening up a new avenue for selective H2S detection under physiological pH and controllable 1O2 release.

Iminocoumarin-based red to near-infrared fluorescent turn-on probe with a large Stokes shift for imaging H2S in living cells and animals

The development of organic dye-based fluorescent probes for detection of H2S in living systems has attracted considerable attention in recent years. In this work, a novel tetrahydroquinoxaline iminocoumarin-based red to near-infrared (red-to-NIR) fluorescent probe was developed for detection of H2S. This probe shows a rapid and distinct red-to-NIR fluorescent turn-on detection process for H2S with high selectivity and sensitivity (the detection limit was determined to be as low as 10 nM at physiological pH). In addition, this probe exhibits a remarkable large Stokes shift (128 nm) and low cytotoxicity, and can be applied for imaging H2S in living cells and animals. All these results demonstrated that this new probe is promising for detection of H2S both in vitro and in vivo.

A novel xanthene-based colorimetric and fluorescence probe for detection of H2S in living cells

Hydrogen sulfide (H2S) is a signaling gasotransmitter that plays an important role in modulating the functions of different systems. We have reported a xanthene-based colorimetric and fluorescence Probe A for the selective detection of H2S over other sulfide-containing analytes. The emission intensity increases linearly with NaSH concentrations in the low concentration range suggested that it shows high sensitivity toward H2S with a detection limit as low as 7 nM. This probe exhibits an enhanced up to 250-fold fluorescence enhancement at λem 510 nm in the presence of H2S (50 μΜ). In addition, Probe A can be applied for fluorescent imaging of H2S in living cells, providing a potentially powerful molecular imaging tool for imaging H2S.

“Turn-on” fluorescent probe for detection of H2S and its applications in bioimaging

A novel fluorescent probe (named YQ-1) containing disulfide-bond coumarin derivative was developed for H2S. In response to H2S, YQ-1 showed remarkable fluorescent emission enhancement at 462nm. Besides, YQ-1 exhibited higher selectivity, faster response rate, low cytotoxicity and low detection limit (0.052μM). Further, YQ-1 was used to detect the presence of H2S level in living A549 cells, indicating YQ-1 has good membrane permeability and fluorescence properties.

Cascade reaction-based fluorescent probe for detection of H2S with the assistance of CTAB micelles

We report a turn-on fluorescent probe for H2S through a cascade reaction using a new trap group 4-(bromomethyl)benzoate, based on excited-state intramolecular proton transfer (ESIPT) sensing mechanism. The probe showed good selectivity and high sensitivity towards H2S and it was capable of detecting and imaging H2S in living HeLa cells, indicating its potential biological applications.

A coumarin-based colorimetric fluorescent probe for hydrogen sulfide

A coumarin-based fluorescent probe for selective detection of hydrogen sulfide (H2S) is presented. This ‘off–on’ probe exhibited high selectivity towards H2S in aqueous solution with a detection limit of 30 nM. Notably, because of its dual nucleophilicity, the probe could avoid the interference of thiols and other sulfur containing compounds. A novel coumarin-based, reaction-type fluorescent probe for detection of H2S in aqueous solution was synthesized through a two-step nucleophilic reaction.

OP01 Visible and chemiluminescent methods for H2S detection and quantification

Hydrogen sulfide is an important biological signalling molecule and an important target for environmental detection. A major challenge in developing small-molecule, reaction-based probes for H2S detection and quantification is overcoming the much higher concentration of biological nucleophiles, such as reduced glutathione (GSH). In this presentation, we present two new H2S detection and quantification methods, both of which can be used to measure H2S analytically using common laboratory equipment or, alternatively, can be used for qualitative naked-eye H2S detection. Colorimetric H2S detection and quantification: The developed method utilizes a new strategy for reaction-based H2S detection facilitated by nucleophilic aromatic substitution of H2S onto an electrophilic nitrobenzofurazan (NBD) chromophore. The resultant platform reacts quickly with H2S (